Fiber

Rumen digestibilityBasics

§ Depending on diet formulation, production level and typ 50-80% of nutrients are digested in the rumen.

§ Optimizing rumen nutrition is key in feeding ruminants.§ Digestibility of feeding stuffs impacts highly feed intake and

available energy, thus milk production or growth.§ Fiber content and structure is responsible for ruminal

and total tract digestibility of feeding stuffs.

§ Depending on feeding stuff rate and extent of ruminal fermentation varies.

Rumen digestibilityFermentation rate and extent

0%

5%

10%

15%

20%

25% wheat

grass silage

corn / corn silage / soybean meal

fast fermenting slow fermenting

Rumen digestibilitycarbohydrates

Carbohydrates

Soluble carbohydrates

Sugar Starch

Cell wall carbohydrates (fiber)

Pectin

NDF

Hemi-cellulose

ADF

Cellulose Lignin

indigestible0-20%

medium to good digestible20-90%

very good digestible60-100%

FIBRAMAX

Rumen Fermentation enhancer & Fiber Digestion aid

5

FIBRAMAXDirect Fed Microbials (DFM)

§ Is the fruit of unique combined microbials fermentation to maximize its metabolites content (A. Orizae, K. Marxianus, L. , S. Cerevisiae).

§ The micro-organism culture is blocked when the max thefermentation activity is running. After the inactivation of the cells growth the culture is dried via fluidized bed in order to keep intact the cell structure and the enzymes activity.

§ That way preserves all the biological ingredients of the culture so metabolites, fermentation products as well as enzymes are available and can enhance the GIT micro-flora activity and digestive process. 6

Fermentation technology

7

FIBRAMAXDirect Fed Microbials (DFM)

§ DFM Aspergillus oryzae and Aspergillus niger wichbelong to the families of Trichocomaceae, explicate theirmechanism of action through a complete pool of amylolyticand fibrolitic enzymes, reducing the hydrolysis rates of thestarchy and fibrous components of the ration andincreasing the production of ruminal microbial biomass andvolatile fatty acids.

§ Kluyveromyces and Saccharomyces provide thepeptides and the basic nutrients for the formation of rumencellulolitic bacterial flora.

8

Detachment offorage lignin from

cellulose

Increase activityof key-fiber

digesting micro-organisms

FIBRAMAXThe Goal

Maximizing energy from forage & diet with more milk & more fat

FibramaxDairy cows Experiments

1. In vitro : NDF degradability2. Five field trials combined (Italy) : 2009-103. in vitro: NDF digestibility (B): 2013-034. Field trial (Czech Republic): Sep 20135. Field trial (Germany): Aug /Dec 20136. Field trial (The Netherlands): Mrz 20147. Field trial (USA): Apr 2014

8. University trial (I): 20149. in vitro: Ruminal fermentation (USA): 2016

10

In vitro NDF testing

20,9

30,235,6

58,0

13,9

27,1 30,2

43,1

0,0

10,0

20,0

30,0

40,0

50,0

60,0

70,0

Straw Alfalfa Hay Corn Silage Soybean hulls

FIBRAMAX Control

NDF Degradation % (in rumen fluids – 24 h)

11

In vitro NDF testingBenefits

§ Enhanced NDF digestibility: +10-50%§ Effect on digestible feeding stuffs as well as on low

digestible ones.

12

Combined Field Trials ResultsItaly – 2009/2010

§ Average of 5 trial (772 dairy cows in total)§ Dosage of Fibramax: 20 g/cow/day

§ Trial duration: first 112 days in milk

13

field trial Czech Republic – 2013-09trial design

§ Fibramax groups:§ Dosage of Fibramax: 15 g/cow/day§ 3 weeks feeding§ approx. 150 dairy cows§ 3 lots F11, F12, and F14

§ Control groups:§ approx. 140 dairy cows§ 3 lots: C3, C4 and C5§ Dry matter intake: 24 k/cow/day

14

Field trial Czech Republic – 2013-09diet characteristics

drymatterintake,

kg/cow/dayCornsilage 3,68Grasssilage 0,70Grasshay 1,35Luzernehay 2,70Straw 0,36Spentbrewersgrain 2,40compoundfeed 6,75Proteinconcentrate 1,80Rapeseedmeal 1,26Cottonseed 1,62DDGS–fromcorngrain 0,09Protectedfat 0,19Molassses 1,20

MJ/kgorMJ/kg

NEL,MJ/kg 6,90Crudeprotein,g/kg 206RumenUdegradableProtein,g/kg 65Crudefiber,g/kg 165calculated

Field trial Czech Republic – 2013-09Results

16

37,037,538,038,539,039,540,040,541,041,542,0

7/Sep/13 14/Sep/13 21/Sep/13 28/Sep/13 5/Okt/13 12/Okt/13

Avg.DailyMilkControl

Avg.DailyMilkFibramaxwithoutlotnum.13

Field trial Czech Republic – 2013-09Results – at similiar day in milk

39

40

41

42

43

44

45

46

7/ Sep/ 13 14/ Sep/ 13 21/ Sep/ 13 28/ Sep/ 13 5/ Okt/ 13 12/ Okt/ 13

controlFibramax

Field trial Czech Republic – 2013-09Results – effect due to day in milk

0,0

0,5

1,0

1,5

2,0

2,5

0 30 60 90 120 150

milk, kg/c/d

day in milk

Fibramax

Fibramax

Field trial Czech Republic – 2013-09Benefits

§ After about 3 week more milk in all lots fed with Fibramax§ improved milk production: +1,4 kg/cow/day

§ Difference in milk at end of trial to the beginning, corrected by results of control group

§ Main benefits at early production: +2 kg/cow/day§ Less variation in milk production

19

Field trial Germany, 2013-08/12Results 2

20

Milkkg/cow/day

ECMkg/cow/day

Fat%

Protein%

Fatkg/cow/day

Proteinkg/cow/day

Jun 13 22,4 22,2 4,12% 3,04% 0,92 0,68Jul 13 23,4 23,3 4,06% 3,18% 0,95 0,74Aug 13Sep 13 21,2 22,3 4,46% 3,34% 0,95 0,71Okt 13 21,6 22,7 4,40% 3,48% 0,95 0,75Nov 13 23,1 24,2 4,34% 3,48% 1,00 0,80Dez 13 23,5 24,7 4,38% 3,47% 1,03 0,82Jan 14 23,6 24,2 4,22% 3,39% 1,00 0,80Feb 14 23,7 23,7 4,01% 3,35% 0,95 0,79

Field trial Germany, 2013-08/12Benefits

§ Improved milk volume even at low producing herd§ Biggest benefit at summer time at heat stress

§ After about 3 week more milk in all lots fed with Fibramax§ improved milk production: summer: +1,6 kg/cow/day

winter: +0,2 kg/cow/day§ Main effect in summer time at heat stress = balanced heat stress +

improved milk production§ Impact on milk protein and fat in summer and winter feeding.

21

Field trial The Netherlands, 2014-03Design

§ Dairy farm in The Netherlands§ Mainly grassland§ Trial purpose

§ Test fiber digestion by manure sieving§ Trial Dosage:

§ 15 g/cow/day§ Off – on design

Field trial The Netherlands, 2014-01Results

23300

310

320

330

340

350

360

370

380

390

400

1/ Feb/ 14 8/ Feb/ 14 15/ Feb/ 14 22/ Feb/ 14 1/ Mär/ 14 8/ Mär/ 14

effect of Fibramax on faezes fiber0 g/c/d

15 g/c/d

-7%

Field trial The Netherlands, 2014-03Results

§ Less fiber in the faeces§ Better feed digestibility

§ More net energy out of the feeding stuffs§ Improved feed intake and milk production can be expected

24

Feeding Instructions

§ Designed for feeding 14 days prior to calving continuingat least for 120 days. To be incorporated to compoundfeed, premixture or TMR

§ Dairy cows : 10-30 g/h/d

§ Beef Cattle: 4-10 g/h/d§ Small ruminant (sheep & goat) : 2-5 g/h/d§ We suggest to administer at least 20 g/head/day, but better

results can be obtained using 30 g/head/dayDepending on the feeding plan and condition of the cow the dosages should be adapted

25