Metode Supercritical Fluid Extraction Menurut Jurnal Internasional

Metode Thin-Layer Chromatography yang digunakan di Jurnal InternasionalFarahia Khairina Widyaningrum 1306480080{Lipophilicity study of fifteen fluoroquinolones by reversed-phase thin-layer chromatography

Urszula Hubicka,Barbara Zuromska-Witek, ukasz Komstab and Jan Krzeka Judul Jurnal

Identitas yang penting dalam sebuah senyawa yang memiliki aktivitas biologis

Passive transport of drug through biological membranesBiasanya digambarkan dengan Koefisien Partisi LipofilisitasTidak memakan waktu yang banyak dibandingkan metode yang lain.Eluen yang bisa digunakan sangat bervariasiPemisahan antara fase gerak dan fase diam di TLC mirip dengan pemisahan yang ada di dalam tubuhMengapa TLC ?Membandingkan lipofilitas dari lima belas fluoroquinolones dengan Reversed-phase TLC dan dihitung menggunakan program TujuanAcetoneAcetonitrileMethanolChloroform2-propanol of analytical grade (purchased from Chempur)TetrahydrofuranHPLC grade water, filtered through a 0,2 m filter before useReagenBalofloxacin (BAL)Ciprofloxacin (CIP)Danofloxacin (DAN)Difloxacin (DIF)Enrofloxacin (ENR)Flumequine(FLU)Lomafloxacine (LOM)Marbofloxacin (MAR)Standard SubstanceMoxifloxacin (MOX)Norfloxacin (NOR)OfloxacinOrbifloxacin (OFL)Pefloxacin (PEF)Prulifloxacin (PRU)Sparfloxacin (SPA)ENR, FLU methanol contaioning 0,40 mL chloroform 1.00 mg mL-1PRU methanol containing 3,5 ml of acetonitrile 1.00 mg mL-1MAR methanol : water 1:1 , 1.00 mg mL-1Standard SolutionsBAL, CIP, DIF, LOM, MOX, NOR dan PEF Methanol 1.00 mg mL-1DAN, ORB, SPA methanol : chloroform 1 :1 v/v 1.00 mg mL-1OFL water : acetonitrile 1:6 v/v 1.00 mg mL-1Solutions of BAL, CIP, DIF, LOM, MOX, NOR and PEF in methanol were prepared at a concentration of 1.00 mg mL1. Solutions of DAN, ORB and SPA in the mixture of methanol : chloroform 1 : 1 v/v were prepared at a concentration of 1.00 mg mL1. Solution of OFL in the mixture of water : acetonitrile 1 : 6 v/v was prepared at a concentration of 1.00 mg mL1. Solu- tions of ENR and FLU in methanol containing 0.40 mL of chlo- roform were prepared at a concentration of 1.00 mg mL1. Solution of PRU in methanol containing 3.5 mL of acetonitrile was prepared at a concentration of 1.00 mg mL1. Solution of MAR in the mixture of methanol : water 1 : 1 v/v was prepared at a concentration of 1.00 mg mL1. 9Precoated TLC sheets of RP-18 silica gel 60 with fluorescent indicator on aluminium 10 x 10 cm cut from 20 x 20 cm)Four microliters of test solutions were applied using a Linomat V (Camag)5mm bands with a distance of 10.00 mm from pate bottom10mm from the edge of the plate5 mm between the edges of the bandsChamber (20x10x18 cm) 20 Celcius degree

TLC Conditions

Mobile PhaseThe investigation was performed using five various aqueous mobile phases each containing different proportions of organic modifiers. The RM values, which are necessary for further calculations, were obtained on the basis of formula It was found that the RM values decreased linearly with increasing concentration of the organic modifier in mobile phase, and could be described by formula

Result