gem presentation
DESCRIPTION
GEM Presentation. G ermplasm E nhancement of M aize. GEM Team USDA-ARS NCRPIS GEM - December 8, 2004 -. Quality Trait Research. Susan Duvick Corn Quality Traits Lab Manager. Wet Chemistry Laboratory. Personnel. Susan Duvick, Laboratory Manager (NCRPIS) - PowerPoint PPT PresentationTRANSCRIPT
GEM Presentation
GEM TeamUSDA-ARS NCRPIS GEM
- December 8, 2004 -
Ger
mp
lasm
En
han
cem
ent
of M
aize
Quality Trait Quality Trait ResearchResearchSusan DuvickSusan Duvick
Corn Quality Traits Lab Corn Quality Traits Lab ManagerManager
Wet Chemistry Laboratory
PersonnelPersonnel
Susan Duvick, Laboratory Manager Susan Duvick, Laboratory Manager (NCRPIS)(NCRPIS)
Kaily Chang, Research Associate (CICGRU)Kaily Chang, Research Associate (CICGRU) Oswaldo Taboada-Gaytan, Graduate Student Oswaldo Taboada-Gaytan, Graduate Student
(CICGRU)(CICGRU) Joe Van Gorp, Undergraduate Intern Joe Van Gorp, Undergraduate Intern
(CICGRU)(CICGRU) Carli Petersen, Undergraduate Intern Carli Petersen, Undergraduate Intern
(NCRPIS)(NCRPIS) Cooperating scientists and project membersCooperating scientists and project members
Corn Quality Traits LaboratoryCorn Quality Traits Laboratory
Primary FunctionsPrimary Functions Germplasm evaluation for quality traitsGermplasm evaluation for quality traits Method development for new quality traitsMethod development for new quality traits Research leading to high quality corn germplasmResearch leading to high quality corn germplasm
CRIS ProjectsCRIS Projects Germplasm enhancement of maize project (GEM) Germplasm enhancement of maize project (GEM)
(Mike Blanco) (Mike Blanco) Enhancing corn germplasm for quality traits Enhancing corn germplasm for quality traits
(Linda Pollak)(Linda Pollak) Breeding high-quality corn for sustainable, low-Breeding high-quality corn for sustainable, low-
input farming systems (Linda Pollak)input farming systems (Linda Pollak) Quantitative genetics and grain quality of corn Quantitative genetics and grain quality of corn
(Paul Scott and Jode Edwards)(Paul Scott and Jode Edwards)
Current Analytical MethodsCurrent Analytical Methods Whole grain composition (POS) by NIR using sample Whole grain composition (POS) by NIR using sample
sizes from 1 kernel to 1 poundsizes from 1 kernel to 1 pound Specific density by NIR using sample sizes from 1 ear Specific density by NIR using sample sizes from 1 ear
to 1 poundto 1 pound Extractable starch by NIR using sample sizes from 1 Extractable starch by NIR using sample sizes from 1
ear to 1 poundear to 1 pound Starch thermal properties by DSC using sample sizes Starch thermal properties by DSC using sample sizes
from 1 kernel to bulk starch samplesfrom 1 kernel to bulk starch samples Starch viscosity properties by RVA using sample sizes Starch viscosity properties by RVA using sample sizes
of starch from at least 10 grams of cornof starch from at least 10 grams of corn Fatty acids by GC from single kernelsFatty acids by GC from single kernels Wet milling by both bench top single kernels and Wet milling by both bench top single kernels and
100g using a starch table100g using a starch table
NIR Whole Grain Composition
NIR Sample SizesNIR Sample Sizes
GEM Results-CompositionGEM Results-Composition
Protein > 15% Protein > 15% Oil > 4%Oil > 4% Starch > 71%Starch > 71% B73 values: 14% protein, 3.5% oil, B73 values: 14% protein, 3.5% oil,
70% starch 70% starch
Starch ExtractionStarch Extraction
Bench Top Wet MillingBench Top Wet Milling
100g Wet Milling
Starch Thermal Analysis
DSC Curve DSC Curve CH05015:N1204-57-1-B-B
GEM Results-Starch QualityGEM Results-Starch Quality
ToG < 62ToG < 62ooCC TrG < 5TrG < 5ooCC %R > 60%%R > 60% Commercial corn starch: ToG, TrG, Commercial corn starch: ToG, TrG,
%R%R
Field Day 2004Field Day 2004
Recommended GEM Lines for 2004Recommended GEM Lines for 2004
Pedigree Origin (race) SET Comment
BARBGP:N08a18-332-1-B
Barbados (Tuson) 2004 S4elevated starch and
oil
CH05015:N1502-86-1-B
Chile (Camelia) 2004 S4 elevated protein
CHIS775:S1911b-120-1-B-B
Mexico (Tuxpeno) Set Relevated oil and
protein, high TpG, flat-top peak
DK212T:N11a12-191-1-B
Thailand (comm. DK) 2004 S4 high starch
DKB844:S1601-73-1-B-B
Mexico (comm. DK) Set Elow gel onset temp; low PHI, wide RnG
DREP150:N2011d-624-1-B
Domin. Rep. (Mixed) 2004 S4 elevated protein
UR11003:S0302-1011-1-B
Uruguay (Dente Branco)
Set Hnarrow range of
gelatinization
DKB844:S1601-3-2 Mexico (comm. DK) Set EElevated protein, low gel temp onset, low RnG, high % retro
CH05015:N1204-57-1 Chile (Camelia) Set HElevated protein and oil, very high TpG,
large complex peak
New MethodologyNew Methodology
ETOH by bench top laboratory procedureETOH by bench top laboratory procedure Food applications of oils with altered fatty Food applications of oils with altered fatty
acidsacids Calculating breeding parameters for Calculating breeding parameters for
quality traitsquality traits Modeling from our existing data to predict Modeling from our existing data to predict
resistant starchresistant starch Thermoelectric conductivity and Thermoelectric conductivity and
electroactive transportelectroactive transport Plasticizing starches Plasticizing starches
GEM Presentation
The End
Ger
mp
lasm
En
han
cem
ent
of M
aize