gowanus canal & pathomap - metasubmetasub.org/wp-content/uploads/2016/05/session1_jorgensen.pdf ·...
TRANSCRIPT
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• Methods – DNA Extraction using MoBio Powersoil Kit – Illumina Prep – HiSeq Sequencing – MetaPhlAn for preliminary analysis
• 124 taxa, 33 species found – 18 new species not seen on the subway
• Additional collections in winter 2014 and spring 2015
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Taxa Breakdown
Archaea, 2%
Bacteria, 91%
Unclassified, 7%
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Potential Pathogens
Pseudomonas putidas
Escherichia coli
Enterococcus faecalis
Propionibacterium acnes
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DNA Yield and Species Diversity
0
10
20
30
40
50
60
70
80
90
100
GC01 GC02 GC03 GC04 GC05GC06
GC07GC08
GC09GC10
GC11GC12
GC13GC14
Pe
rce
nta
ge (
%)
DNA Yield
Number of Species
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Eubacterium_halliiMethanocorpusculum_labreanumFaecalibacterium_unclassifiedMethanosarcina_acetivoransNakamurella_multipartitaEscherichia_unclassifiedBifidobacterium_adolescentisEnterococcus_unclassifiedPropionibacterium_unclassifiedMethanosarcina_unclassifiedPseudoalteromonas_unclassifiedEnterobacter_unclassifiedEnterococcus_faecalisMycobacterium_gilvumCellulophaga_unclassifiedEubacterium_rectaleRuminococcus_bromiiCitrobacter_unclassifiedPropionibacterium_acnesPantoea_vagansMethanobrevibacter_unclassifiedPseudomonas_putidaEscherichia_coliErwinia_billingiaeBifidobacterium_unclassifiedMethanobrevibacter_smithiiSulfurimonas_unclassifiedCandidatus_Pelagibacter_ubiqueDesulfococcus_oleovoransPseudomonas_unclassifiedBeggiatoa_unclassifiedMycobacterium_unclassifiedDesulfobacterium_autotrophicum
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Cornell Team Sequencing Chris Mason Data Analysis & Visualization Elizabeth Henaff Ebrahim Afshinnekoo
Genspace Team Sample Collection & Processing Ellen Jorgensen Julie Wolf Eric Fernandez Ashley Quinn Heather Dewey-Hagborg and a host of others… Data Analysis
Robert Young
NBW Team Sample Collection & Data Visualization Ian Quate Tom Woltz Matthew Siebert