how do i perform a lumbar puncture

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CLINICIAN’S CORNER THE RATIONAL CLINICAL EXAMINATION How Do I Perform a Lumbar Puncture and Analyze the Results to Diagnose Bacterial Meningitis? Sharon E. Straus, MD, MSc, FRCPC Kevin E. Thorpe, MMath Jayna Holroyd-Leduc, MD, FRCPC PATIENT SCENARIO A previously healthy 70-year-old woman presents to the emergency de- partment with a 3-day history of fe- ve r, co nfu sion, and le tha rg y. She is un- able to cooperate with a full physical examination, but she has neck stiff- ness upon neck flexion. Her score on the Glasgow Coma Scale is 13 (eye, 4; ver bal, 4; motor , 5) . The fin dingsfro m a chest radiograph and urinalysis are normal. 1 You seek consent from her husband to perform a lumbar punc- ture (LP). Why Is This Diagnostic Procedure Important? In a pre vio us Ration al Clinic al Exa mi - natio n artic le, Attia and coll eague s 1 dis- cussed the above scenario and recom- men dedproceeding to LP for def ini tiv e testing of the cereb rosp inal fluid (CSF). Cerebrospinal fluid is a clear, color- less fluid that fills the ventricles and subarachnoid space surrounding the brain and spinal cord. 2 Lumbar punc- ture allows this fluid to be sampled, facilitating the diagnosis of various conditions. Since it was first described by Quincke 3 in 1891, the LP has become See also Patient Page. CME available online at www.jama.com Autho r Affiliati ons: Divi sionof Gen eralIntern al Med i- cine,Universi tyof Calgary , Albert a (DrStraus);Knowledge Transla tion Program, University of Toront o/St Mi- chael’sHospital,Toron to,Ontario(DrsStrausandHolroyd- Leduc and Mr Thorpe); Department of Public Health Sciences, University of Toronto, Toronto, Ontario (Mr Thorp e); Divisionof General Interna l Medicin e, Univer- sity HealthNetwork, Toronto,Ontario (Dr Holroyd-Leduc) . Corresponding Author: Sharon E. Straus, MD, MSc, FRCPC,Foothill s Medic al Centre140329th St NW,Cal- gary , Cana da AB T2N2T9 (sha ron.straus @utor onto .ca). The Rational Clinical Examination Section Editors: David L. Simel, MD, MHS, Durham Veterans Affairs Medical Centerand Duke Unive rsity Medical Center , Durham, NC; Drummond Rennie, MD, Deputy Edi- tor, JAMA. Context Diagnostic lumbar punctures (LPs), commonly used to rule out meningitis, are associated with adverse events. Objective To syst emati call y review the evid ence abou t diagnostic LP tech niqu es that may decrease the risk of adverse events and the evidence about test acc ur acy of cere- brospinal fluid (CSF) analysis in adult patients with suspected bacterial meningitis. DataSources We searched theCochr ane Lib rar y, MEDLINE (usingOvid andPubMed) from 1966 to January 2006 and EMBASE from 1980 to January 2006 without lan- guage restrictions to identify relevant studies and identified others from the bibliog- raphies of retrieved articles. Study Selection We included randomized trials of patients aged 18 years or older undergoing interventions to facilitate a successful diagnostic LP or to potentially re- duc e adv ers e events. Stu die s assess ing the accu racy of bio che mic al ana lys is of the CSF for possible bacterial meningitis were also identified. Data Extraction Two investigators independently appraised study quality and ex- tracted relevant data. For studies of the LP technique, data on the intervention and the outcome were extracted. For studies of the laboratory diagnosis of bacterial men- ingitis, data on the reference standard and test accuracy were extracted. Data Synthesis We found 15 randomized trials. A random-effects model was used for qua nti tat ive syn the sis. Fiv e stu die s of 587patients compar ed atra uma tic nee dle s wit h standard needles and found a nonsignificant decrease in the odds of headache with an atraumatic needle (absolute risk reduction [ARR], 12.3%; 95% confidence interval [CI], 1.72% to 26. 2%) . Reinsertion of the sty let bef ore nee dle remova l dec rea sed the ris k of headache (ARR, 11.3%; 95% CI, 6.50%-16.2%). The combined results from 4 studies of 717 pat ients sho wed a non sig nif ica nt decr eas e in hea dac he in pat ien ts who wer e mo- bilized after LP (ARR, 2.9%; 95% CI, 3.4 to 9.3%). Four studies on the accuracy of bio che mic al ana lys is of CSF in pat ien ts wit h sus pec ted meni ngi tis met inc lus ioncriteria. A CSF–blood glucose ratio of 0.4 or less (likelihood ratio [LR], 18; 95% CI, 12-27]), CSF white blood cell count of 500/µL or higher (LR, 15; 95% CI, 10-22), and CSF lactate level of 31.53 mg/dL or more (3.5 mmol/L; LR, 21; 95% CI, 14-32) accurately diag- nosed bacterial meningitis. Conclusions These data suggest that small-gauge, atraumatic needles may de- crease the risk of headache after diagnostic LP. Reinsertion of the stylet before needle removal shoul d o ccur and patien ts do not require bed rest after the procedure. Future research should focus on evaluating interventions to optimize the success of a diag- nostic LP and to enhance training in procedural skills.  JAMA. 2006;296:2012-2022 www.jama.com 2012 JAMA, October 25, 2006—Vol 296, No. 16 (Reprinted) ©2006 American Medical Association. All rights reserved.  at TEXAS TECH HLTH SCI CTR, on December 15, 2006 www.jama.com Downloaded from 

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Page 1: How Do I Perform a Lumbar Puncture

8/9/2019 How Do I Perform a Lumbar Puncture

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CLINICIAN’S CORNERTHE RATIONAL

CLINICAL EXAMINATION

How Do I Perform a Lumbar Punctureand Analyze the Results to DiagnoseBacterial Meningitis?Sharon E. Straus, MD, MSc, FRCPC

Kevin E. Thorpe, MMath

Jayna Holroyd-Leduc, MD, FRCPC

PATIENT SCENARIO

A previously healthy 70-year-oldwoman presents to the emergency de-partment with a 3-day history of fe-ver, confusion, and lethargy. She is un-able to cooperate with a full physicalexamination, but she has neck stiff-ness upon neck flexion. Her score onthe Glasgow Coma Scale is 13 (eye, 4;verbal, 4; motor, 5). The findings froma chest radiograph and urinalysis arenormal.1 You seek consent from herhusband to perform a lumbar punc-ture (LP).

Why Is This Diagnostic Procedure

Important?

In a previous Rational Clinical Exami-nation article, Attia and colleagues1 dis-cussed the above scenario and recom-mended proceeding to LP for definitivetesting of the cerebrospinal fluid(CSF).Cerebrospinal fluid is a clear, color-less fluid that fills the ventricles andsubarachnoid space surrounding thebrain and spinal cord.2 Lumbar punc-ture allows this fluid to be sampled,facilitating the diagnosis of various

conditions.Since it was first described by

Quincke3 in 1891, the LP has become

See also Patient Page.

CME available online atwww.jama.com

Author Affiliations: Divisionof GeneralInternal Medi-cine,Universityof Calgary, Alberta (DrStraus);KnowledgeTranslation Program, University of Toronto/St Mi-chael’sHospital,Toronto,Ontario(DrsStrausandHolroyd-Leduc and Mr Thorpe); Department of Public HealthSciences, University of Toronto, Toronto, Ontario (Mr Thorpe); Divisionof General Internal Medicine, Univer-sityHealthNetwork, Toronto,Ontario (DrHolroyd-Leduc).

Corresponding Author: Sharon E. Straus, MD, MSc,FRCPC,Foothills Medical Centre140329th St NW,Cal-gary, Canada AB T2N2T9 ([email protected]).The Rational Clinical Examination Section Editors:David L. Simel, MD, MHS, Durham Veterans AffairsMedical Centerand Duke University Medical Center,Durham, NC; Drummond Rennie, MD, Deputy Edi-tor, JAMA.

Context Diagnostic lumbar punctures (LPs), commonly used to rule out meningitis,are associated with adverse events.

Objective To systematically review the evidence about diagnostic LP techniques thatmay decrease the risk of adverse events and the evidence about test accuracy of cere-brospinal fluid (CSF) analysis in adult patients with suspected bacterial meningitis.

Data Sources We searched theCochrane Library, MEDLINE (usingOvid and PubMed)from 1966 to January 2006 and EMBASE from 1980 to January 2006 without lan-guage restrictions to identify relevant studies and identified others from the bibliog-raphies of retrieved articles.

Study Selection We included randomized trials of patients aged 18 years or older undergoing interventions to facilitate a successful diagnostic LP or to potentially re-duce adverse events. Studies assessing the accuracy of biochemical analysis of the CSFfor possible bacterial meningitis were also identified.

Data Extraction Two investigators independently appraised study quality and ex-tracted relevant data. For studies of the LP technique, data on the intervention andthe outcome were extracted. For studies of the laboratory diagnosis of bacterial men-ingitis, data on the reference standard and test accuracy were extracted.

Data Synthesis We found 15 randomized trials. A random-effects model was usedfor quantitative synthesis. Five studies of 587 patients compared atraumatic needles with

standard needles and found a nonsignificant decrease in the odds of headache with anatraumatic needle (absolute risk reduction [ARR], 12.3%; 95% confidence interval [CI],−1.72% to 26.2%). Reinsertion of the stylet before needle removal decreased the risk ofheadache (ARR, 11.3%; 95% CI, 6.50%-16.2%). The combined results from 4 studiesof 717 patients showed a nonsignificant decrease in headache in patients who were mo-bilized after LP (ARR, 2.9%; 95% CI, −3.4 to 9.3%). Four studies on the accuracy ofbiochemical analysis of CSF in patients with suspected meningitis met inclusion criteria. ACSF–blood glucose ratio of 0.4 or less (likelihood ratio [LR], 18; 95% CI, 12-27]), CSFwhite blood cell count of 500/µL or higher (LR, 15; 95% CI, 10-22), and CSF lactatelevel of 31.53 mg/dL or more (3.5 mmol/L; LR, 21; 95% CI, 14-32) accurately diag-nosed bacterial meningitis.

Conclusions These data suggest that small-gauge, atraumatic needles may de-crease the risk of headache after diagnostic LP. Reinsertion of the stylet before needleremoval should occur and patients do not require bed rest after the procedure. Future

research should focus on evaluating interventions to optimize the success of a diag-nostic LP and to enhance training in procedural skills.

 JAMA. 2006;296:2012-2022 www.jama.com

2012 JAMA, October 25, 2006—Vol 296, No. 16 (Reprinted) ©2006 American Medical Association. All rights reserved.

 at TEXAS TECH HLTH SCI CTR, on December 15, 2006www.jama.comDownloaded from 

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an important diagnostic tool, particu-larly when considering the diagnosis of meningitis. Evaluation of CSF can helpestablish a diagnosis and guide antimi-crobial therapy. Less commonly, LP isused as part of the diagnostic workup

of patients with suspected subarach-noid hemorrhage, demyelinating dis-ease, and leptomeningeal metastasis.4,5

What Adverse Events Can Result

From an LP?

Bier was the first to report the tech-nique of spinal anesthesia and also pro-vided (through personal experience!)the first description of post-LP head-ache.6 Headache and backache are themost frequently reportedadverseeventsassociated with LP. Headache can oc-cur in up to 60% of patients who un-

dergo the procedure, although esti-mates vary due to differences ininclusion criteria and definitions of headache.7-9 Headache canbe severeanddebilitating10 and is believed to occurbecause of CSF leakage through the du-ral puncture site.11 Backache is less com-mon but canoccur in upto 40%of pa-tients followingLP.7 Rareadverseeventsinclude cerebral herniation, intracra-nial subdural hemorrhage, spinal epi-dural hemorrhage, and infection.11

What Are the Contraindicationsto Performing an LP?

Clinicians often worry that an undetec-ted mass lesion or ventricular obstruc-tion causing raised intracranial pres-sure pose risks for cerebral herniationfollowing an LP.4 However, no conclu-sive evidence supports that the risk canbe reduced with universal neuroimag-ing prior to LP. Insteadof universal neu-roimaging, clinicians can use the clini-cal examination to guide thedecisiontoobtain neuroimaging. In a prospective

study, 113 patients were examined byinternal medicine residents (overseenbyemergency physicians) prior to under-going computedtomography (CT)of thebrain and subsequent LP.12 The me-dian age of patients was 42 years, 36%were immunocompromised,and46%of patients had altered mentation. Alteredmentation (likelihood ratio [LR], 2.2;

95% confidence interval [CI], 1.5-3.2),focal neurologicalfinding (LR,4.3;95%CI, 1.9-10), and papilledema (LR, 11;95% CI, 1.1-115) increased the likeli-hood of an intracranial lesion.12 Over-allclinicalimpression (not defined in the

study) was able to identify patients witha CT-defined contraindicationto LP (LR,19; 95% CI, 4.8-43). In a second pro-spective study of 301 patients with sus-pected meningitis, 235 underwent a CTscan prior to LP.13 The mean age of pa-tients was 40 years (16% were 60years), 25% were immunocompro-mised, and 27% of patients had a Charl-son comorbidity score of more than 1.Patients were assessed clinically by anemergency physician or general inter-nist. The absence of a number of clini-cal features at baseline wasable to iden-

tify those who were unlikely to have anabnormal CT result (LR, 0.10; 95% CI,0.03-0.31).The absence of all of the fol-lowing baseline characteristics was as-sociated with this low LR: age 60 yearsor older, immunocompromised state,history of central nervous system dis-ease, and seizure within 1 week of pre-sentation. In addition, there could benone of the following physical exami-nation findings: abnormal level of con-sciousness, inability to answer 2 ques-tions correctly, inability to follow 2

consecutive commands correctly, gazepalsy, abnormalvisual fields, facial palsy,arm drift, leg drift, and abnormal lan-guage. Using the pretest probability of an abnormal CT finding from this study(23.8%), the absence of all of these fea-tures would reducethe probability of anabnormal finding to 3.0%. The find-ings from these 2 studies have not beenvalidated prospectively in other inde-pendent populations.

Local infection at the puncture siteis also a contraindication to complet-

ing an LP but this occurs infre-quently.4 More frequently,cliniciansareconcerned about coagulation defectsand use of anticoagulants, which mayincrease the risk of epidural hemor-rhage. In 1 study of post-LP complica-tions, outcomes were compared in 166patients receiving anticoagulation with171 of those who were not receiving

therapy. There was a trend toward in-creased risk of paraparesis in the anti-coagulated patients (relative risk, 11.0;95% CI, 0.60-199) with 5 patients inthe anticoagulation group experienc-ing an adverse event compared with

none in the control group. In all pa-tients who experienced paraparesis, an-ticoagulation had been started withinan hour of the procedure.14 A survey of 246 pediatric and adult neurology de-partment chairpersons and residencyprogram directors foundthat 45% of re-spondents ordered platelet and antico-agulation studies prior to LP.15 We wereunable to find any data evaluating thesafety of LP in patients with low plate-let counts. In a case series of 66 pa-tients with acute leukemia, patientswith lower platelet counts (50103 / 

µL) had higher risk of a traumatic pro-cedure as defined by the presence in theCSF of more than 500 red blood cellsper high-powered field.16 However, LPswere not performed in patients withplatelet counts lower than 20103 /µLin this study.

 We conducted a systematic review toidentify studies of interventions that en-hance the success ofan LPand thatmini-mize adverse events. Based on reviewof theevidence andits integration with ex-pert opinion, we provide a best-

practice approach for LP in adults. Be-cause a clinician’s interpretation of theLP results is tightly coupled to the clini-cal examination findings, we also re-viewed the literature that addresses theaccuracy of common CSF tests for bac-terial meningitis. Testsfor diagnosing vi-ral meningitis were not included in thisreview. Although there are other indi-cations for LP and CSF analysis, this ar-ticle focuses on CSF analysis for sus-pected bacterial meningitis because itrequires immediate action and is one of 

the most common diagnoses that gen-eralist physicians consider when per-forming this procedure.

METHODS

Searches of the Cochrane Library,MEDLINE (using Ovid and PubMed)from 1966 to January 2006, andEMBASE from 1980 to January 2006

DIAGNOSING BACTERIAL MENINGITIS BY LUMBAR PUNCTURE

©2006 American Medical Association. All rights reserved. (Reprinted) JAMA, October 25, 2006—Vol 296, No. 16 2013

 at TEXAS TECH HLTH SCI CTR, on December 15, 2006www.jama.comDownloaded from 

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were completed to identify relevantstudies. The search strategy used theterms lumbar puncture, spinal punc-ture, dural puncture, headache, head-ach*, spinal needle*, cerebrospinal fluid,spinal fluid, and meningitis. Interven-

tion studies were limited to random-ized controlled trials using the termsrandomized controlled trial, controlledclinical trial, clinical trial, random allo-cation, and random*. No language re-strictions were used. Additional ar-ticles wereidentified fromsearching thebibliographies of retrieved articles. De-tails on the search strategies are avail-able on request.

Randomized trials of patients (18years) undergoing interventions to po-tentially reduce headache and back-ache at the time of diagnostic LP were

included. However, if no randomizedstudies of a particular interventionwereidentified, studies of lower quality—including cohort, case-control, and caseseries—were retrieved. Studies assess-ing patients undergoing LP during spi-nal anesthesia or myelography were ex-cluded because these procedures areclinically different froma diagnostic LP.Smaller amounts of fluid are removedduring spinal anesthesia and myelogra-phy than with diagnostic LP and fluidsare inserted during these other proce-

dures. Moreover, the risk of headacheis greater with diagnostic LP than withspinal anesthesia.7 Interventions of in-terest included those that could be usedat thetimeof LP, such as immediate mo-bilization, atraumatic needles, and re-insertion of the stylet. We also at-tempted to identifystudiesthat assessedthe impact of positioning of the patientandexperienceof the operator. The out-come of interest included headache oc-curring up to 7 days after LP.

To examine the accuracy of CSF

analysis in patients with suspected acutebacterial meningitis, we included stud-ies of predominantly adult popula-tions and those that described use of anappropriate reference standard(eg,CSFculture or bacterial antigen) in all pa-tients. In addition, primary data or ap-propriate summary statistics had to beavailable in the studies.

Two reviewers (S.E.S. and J.M.H-L.) independently reviewed and se-lected relevant publications thatmet theinclusion criteria from the search re-sults. Disagreements were resolved byconsensus. In cases of doubt, full-text

articles were retrieved for review anddiscussion. Full-text articles of all ab-stracts that met the inclusion criteriawere retrieved.

The 2 reviewers independently readall full-text articles to confirm that in-clusion criteria were met. The investi-gators also assessed study quality. Forintervention studies, a specially de-signed data collection form wasused toextract data on study quality includ-ing the method of randomization, thepresence of blinding, and the methodused for outcomeassessment. Datawere

also extracted on the intervention andthe dichotomous outcome variable of post-LP headache. The minimum in-clusion criteria for randomized stud-ies of interventions to prevent adverseevents were the description of random-ization and the ability to extract rel-evant patient data. For studies of testaccuracy, datawere extracted on the ref-erence standard, the presence of blind-ing, the index test, and the populationcharacteristics. Minimum inclusion cri-teria for studies of test accuracy in pa-

tients with suspected meningitis werethe completion of an appropriate ref-erence standard in all patients and theability to extract relevant data. Differ-ences in assessment by the reviewerswere resolved through discussion, anda third investigator (K.E.T.) was avail-able if necessary.

For the intervention studies, statis-tical heterogeneity was assessed usingthe method described by Woolf.17 Arandom-effects model (DerSimonianand Laird) was used for quantitative

data. For the studies of test accuracy,LRs were calculated using the random-effects model. Statistical analyses wereconducted using R: A Language and En-vironment for Statistical Computing andthe rmeta contributed package. R is anopen-source dialect of the S language(S was developed by AT&T) that ismaintained by a core team (http://www

.r-project.org). A 2-tailed P value of .05 was considered statistically sig-nificant.

RESULTS

 We found 537 citations of potential in-

terventions to optimize LP technique.Review of these led to retrieval of 22full-text articles for assessment, 15 of which were subsequently identified forinclusion. Reasons for excluding trialswere lack of randomization (5 stud-ies18-22), repeat publication (1 study23),and inability to obtain outcomes data(1 study24). Studies were categorized byintervention including needle type,needle size, reinsertion of stylet, mo-bilization after LP, and use of supple-mental fluids. No studies of other in-terventions—such as positioning of the

patient during LP, direction of bevel,volume of CSF removed, or prophy-lactic use of an epidural blood patch—met the inclusion criteria.

Description of Studies

Fifteen randomized trials were identi-fied with sample sizes ranging from 44to 600people. Eight studies hadsamplesizes of 100 patients or fewer.

Performing the Procedure

Experience of Operator. We were un-

able to identify any randomized stud-ies that evaluated the impact of the ex-perienceof the clinician performing LPson clinical outcomes. Some studies weidentified included experienced neu-rologists,23 whereas others involvedstu-dents under the supervision of physi-cians.25 In a case series of LPsperformedat an urban university-affiliated hospi-tal, the incidence of traumatic LP was15% using a definition of morethan 400red blood cells per high-powered fieldand10% using a definition of more than

1000 red blood cells.

26

However, thelevel of training andspecialty of all phy-sicians were not recorded. One retro-spective study compared the inci-dence of traumatic LP at the end of theresident academic year when house-staff are more experienced with that atthe start of the next year when newhousestaff begin training. Using a cut-

DIAGNOSING BACTERIAL MENINGITIS BY LUMBAR PUNCTURE

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off of 1000 redblood cells/µL, there wasno difference in risk of traumatic LP be-tween experienced housestaff (14%)and inexperienced housestaff (12%).27

In a prospective cohort of 501 patientswho underwent LP either by a nurse,

physician, resident, or medical stu-dent, there was no significant differ-ence in the risk of post-LP headacheamong the3 groups.28 We foundno dataon the number of LPs required to dem-onstrate or maintain proficiency.

Positioning of Patient. We were un-able to identify any studies that evalu-ated the success of LP with different pa-tient positions or the impact of patientpositioning on the risk of adverseevents. One study assessed the inter-spinous distance to determine the im-pactof positioning.Measurementof the

interspinous distance was conductedin16 patients who were placed in 3 po-sitions (lateral recumbent with kneesto chest; sitting and bent forward overan adjustable bedside stand; and sit-ting with feet supported and chest rest-ing on the knees).29 The interspinousdistance was greatest when the patientwas placed in the sitting position withfeet supported.

Needle Choice and Number of Attempts. Five studies with data from587 patients compared atraumatic

Sprotte or Pajunk needles with stan-dard Quincke needles (FIGURE 1) dur-ing diagnostic LP.30-34 One of these stud-ies32 described the randomizationmethod and 4 studies30-33 described theuse of blinded outcomes assessment.Three studies provided data for inten-tion-to-treat analysis.30-32

There was a nonsignificant decreasein the risk of headache among patientswho underwent diagnostic LP with anatraumatic needle (absolute risk reduc-tion [ARR], 12.3%; 95% CI, −1.72% to

26.2%]). There was statistically signifi-cant heterogeneity among these trials(2

4=13.3, P.01). The heterogeneityap-peared to be due primarily to the smallstudy (n=61) by Lenaerts and col-leagues (FIGURE 2) with only 9 out-come events.30 One study32 includeddata on severe headache and foundthisrisk significantly decreased with atrau-

matic needles (ARR, 23%; 95% CI, 6%-40%). There are no data available onhow often an introducer was used withatraumatic needles or its impact.

Three of these studies, which in-volved 296 patients, included data on

the number of attempts required tocomplete the LP when using an atrau-matic needle.31-33 There was no signifi-cant heterogeneity among these stud-ies (2

2 =0.46, P =.80). There was anonsignificant increase in the risk of re-quiring 2 or more attempts when an

atraumatic needlewas used (ARI, 4.9%;95% CI, −13% to 3.4%).31-33 One studyfound an increased risk of requiring 4attempts with an atraumatic needlecompared with standard needle (ARI,14%; 95% CI, 3.1%-25%).32 This study

also included data on backache andfound no increased risk with the atrau-maticneedle(ARR,7.4%; 95% CI, −12%to 27%) despite requiring more at-tempts with an atraumatic needle.These data on backache and number of attempts required with an atraumatic

Figure 1. Types of Lumbar Puncture Needles

StyletNeedle

Standard Needle

 Atraumatic Needle

Two types of lumbar puncture needles are available—the atraumatic (Sprotte or Pajunk) needle and the stan-dard (Quincke) needle. Either the 22-gauge or 20-gauge atraumatic needle, with or without an introducer,can be used for diagnostic lumbar puncture. Use of an atraumatic needle compared with a standard needleand use of a 26-gauge standard needle compared with a 22-gauge standard needle have been shown to beassociated with reduced risk of headache after lumbar puncture.37,38

Figure 2. Atraumatic vs Standard Needles and Occurrence of Any Headache

No. of Patients

With Headache/ 

Total No. of Patients

 Atraumatic

Needle

Better

Standard

Needle

Better

10.001.000.10

Odds Ratio

 Atraumatic

Needle

Standard

NeedleSourceOdds Ratio

(95% Confidence Interval)

3/49 16/50Kleyweg et al,33 1998 0.14 (0.04-0.51)

7/26 2/35Lenaerts et al,30 1993 6.08 (1.14-32.28)

5/50 15/50Muller et al,

31

1994 0.26 (0.09-0.78)14/115 28/115Strupp et al,34 2001 0.43 (0.21-0.87)

21/49 31/48 Thomas et al,32 2000 0.41 (0.18-0.93)

50/289 92/298Overall 0.46 (0.19-1.07)

The size of the data markers reflects the size of the study.

DIAGNOSING BACTERIAL MENINGITIS BY LUMBAR PUNCTURE

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needlewere secondary outcomesof thisstudy and should be further evaluatedin larger trials.

Recently published guidelines from

the American Academy of Neurologysupport the use of atraumatic needleswhen completing diagnostic LPs to re-duce the risk of post-LP headache.35 Inan earlier version of this guideline, theyreported that LP trays containing theSprotte needle are the same price asthose containing the Quincke.36

One study of 100 patients com-pared useof a 26-gaugeQuincke needlevs a 22-gauge Quinckeneedle.37 Blindedoutcomes observers were used and datawere provided for intention-to-treat

analysis. The risk of headache was sig-nificantly reducedwitha smaller needle(ARR, 26%; 95% CI, 11%-40%).

Reinsertion of Stylet

Strupp and colleagues studied 600 pa-tients and compared the effects of rein-sertion of the styletbeforeremoving theatraumatic needle with no reinser-

tion.38 No details were provided on themethod of randomization or the use of blindedoutcomesassessment. Fewer pa-

tients who underwent LP with reinser-tion of the stylet experienced headache(ARR, 11%; 95% CI, 6.5%-16%). It ispostulated that a strand of arachnoidcouldenter theneedlealongwith theoutflowing CSF and if the stylet is not re-placed, thestrand may be threaded backthrough the dura during removal of theneedle, producingprolonged leakage of the CSF. By replacing the stylet beforeremoving the needle, the strand wouldbepushed out and cut, reducing theriskof continued leakage and the resulting

headache.38

Bed Rest After the Procedure

Four studies with data from 717 pa-tients compared immediate mobiliza-tion with bed rest lasting 4 hours forreducing post-LP headache.25,39-41 Onestudy provided some details on themethod of randomization.25 This studyalso described use of blinded out-comes assessors. Two of the 3 studiesprovided data for an intention-to-treat analysis.25,40

There was no significant heterogene-ity among these studies (23 =1.04,

P=.79). There was a nonsignificant de-crease in the risk of headache in pa-tients whoweremobilizedafter LP (ARR,2.9%; 95% CI, ARI 3.4%-ARR 9.3%];FIGURE 3) Three studies looked at headpositioning during bed rest, but giventhat there wasno significant effect from

bed rest, the results of these studieswere excluded fromfurther analysis.42-45

Supplementary Fluids

In a study of 100 patients undergoingdiagnostic LP, Dieterich assessedthe ef-fects of drinking 1.5 L vs 3 L of fluidsper day on risk of post-LP headache.45

No details were providedon themethodof randomization or the use of blindedoutcomes assessors. Thenumber of out-comes events was too small to detect adifference between these groups (riskdifference, 0.0; 95% CI, ARI 18.8%-ARR 18.8%). Sudlow and Warlow46

conducted a systematic review of mo-bilization and fluids for preventingpost-LP headache and found no effecton post-LP headache.

Interpreting the ResultsManeuvers During the Procedure. Nor-mal resting CSF pressure is assumed tobe 60 to 180 mm of H2O or 6 to 14mm Hg.47,48 In the single identifiedstudy, CSFpressure changed little(1.1mmof water) with flexion of the lowerextremities.49 Various maneuvers, suchas compressing the abdomen or the  jugular vein (Queckenstedt’s maneu-ver50), can increase CSF pressure.51 Anobstruction to CSF flow prevents thenormal rise and fall in pressure (posi-

tive Queckenstedt), but we were un-able to find any studies describing theaccuracy of this maneuver for detec-tion of CSF outflow obstruction.

Laboratory Tests

Although CSF samples can be sub- jected to various analyses, we focusedon testresults immediately relevantanduseful to generalist physicians whenevaluating a patient suspected of hav-ing bacterial meningitis. Normal CSFvalues are listed in TABLE 1, but these

values may vary across different labo-ratories.52 We found 460 diagnostic ar-ticles in our literature search, and 6 metinclusion criteria (TABLE 2). One ex-pert suggests that the white blood cellcount be corrected for the presence of red blood cells by subtracting 1 whiteblood cell from the total white bloodcell count in the CSF for every 700 red

Figure 3. Mobilization vs Bed Rest and Occurrence of Any Headache

No. of Patients

With Headache/ 

Total No. of Patients

Mobilization

Better

Bed Rest

BetterMobilization Bed RestSourceOdds Ratio

(95% Confidence Interval)

34/82 34/78Dieterich and Brandt,40 1985 0.92 (0.49-1.72)

4/26 2/23Johansson et al,41 1992 1.91 (0.32-11.54)70/150 79/150 Vilming et al,25 1998 0.79 (0.5-1.24)

15/104 19/104 Vimala et al,39 1998 0.75 (0.36-1.58)

123/362 134/355Overall 0.84 (0.6-1.16)

10.001.000.10

Odds Ratio

The size of the data markers reflects sample size of the study.

Table 1. Reference Values for AdultCerebrospinal Fluid52

Cerebrospinal

Fluid Variable Value

Pressure 60 to 180 mm H2O;6 to 14 mm Hg

Glucose 45-80 mg/dL (2.5-4.4mmol/L)

Protein 15-45 mg/dLBlood-glucose ratio 0.6White blood cell count 5/µLGram stain No organismsCytology No atypical cells

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blood cells.2 He also states that a singlepolymorphonuclear cell in theCSFwitha white blood cell count of less than 5µL is considerednormal. Steeleandcol-leagues suggest rapid analysis of CSFand noted that neutrophil counts can

decrease by 50% within 2 hours of collection.59

 We found 3 studies that met the in-clusion criteria and that described theaccuracy of CSF Gram stain for diag-nosing bacterial meningitis.53-55 Noneof these studies appeared to be pro-spective. All studies reported sensitiv-ity of Gram stain and 1 study reportedspecificityof thistest53 (TABLE 3). Ifbac-teria are seen on Gram stain, it helpsdiagnose bacterial meningitis but if thistestis negative, bacterial meningitis can-not be ruled out.

 We identified 4 studies that met theinclusion criteria and that reported onthe accuracy of biochemical analysis of CSF in patients with suspected centralnervous system infection55-58(Table 2and TABLE 4). Only 1 study of CSFwhite blood cell counts met our strictinclusion criteria. A CSF white bloodcell count of 500/µL or higher in-creasesthe likelihood of meningitis(LR,15; 95% CI, 10-22), whereas a countless than 500/µL lowers the likelihood(LR, 0.3; 95% CI, 0.2-0.4).56

A CSF–blood glucose ratio of 0.4 orless was accurate for diagnosing bac-terial meningitis (LR, 18; 95% CI, 12-27), whereas a normal CSF–blood glu-coseratio made thisdiagnosis lesslikely(LR, 0.31; 95% CI, 0.21-0.45).56,57 ACSF lactate level of 31.53 mg/dL ormore (3.5 mmol/L) was accurate fordiagnosing bacterial meningitis (LR,21;95% CI, 14-3255-57; Table 4), whereasa CSF lactate level of less than 31.53mg/dL (3.5 mmol/L) makes the di-agnosis of bacterial meningitis less likely

(LR, 0.12; 95% CI, 0.07-0.23).Prediction Models

Spanos and colleagues60 developed aprediction rule for diagnosing bacte-rial meningitis.This rule (TABLE 5) wasderived from a retrospective chart re-view of patients with a final diagnosisof acute meningitis. The sample wasdi-

vided into a derivation and validationset, but data from a large number of charts (94/214) in the derivation set hadmissing data and were excluded fromthe analysis. And, the technique usedfor CSF cell count changed during the

study period, which could influence theresults of this study. The accuracy asmeasured by the area under the re-ceiver operating curve (AUC) was 0.97for the validation set.

Hoen and colleagues61 attempted tovalidate the above rule in a retrospec-tive review and used the same data togenerate their own decision rule(Table 5) that included 4 different clini-cal variables. In their validation of theworkby Spanos andcolleagues, theAUCwas 0.98 while that for their derivedequation was0.99. In anotherretrospec-

tive review of patients with meningitis,Leblebicioglu and colleagues62 assessedthe rules by Hoen and Spanos and theAUC was 0.99 and 0.95, respectively.

McKinney and colleagues63 obtainedsimilar results in their retrospectivereview.

In the only prospective validationthat we were able to identify, Baty andcolleagues64 assessed Hoen’s rule in a

sample of 109 patients aged 1 to 85years with acute community-acquiredmeningitis. Data are only available inpatients with bacterial and viral men-ingitis, and thus the specificity of themodel cannot be calculated. The sen-sitivity of their computed model was80% for the diagnosis of bacterial men-ingitis. For this decision rule to be rec-ommended in clinical practice, it needsto be validated prospectively in largerpopulations with broader diseasespectrum.65

Brivet and colleagues66 completed a

retrospective study and found that thepresence of at least 1 sign of severity of disease at the time referral and a CSFneutrophil count of more than 1000/µL

Table 2. Studies Assessing Cerebrospinal Fluid Analysis in Patients With Suspected CentralNervous System Infection

Source Study Design Sample Size Age Reference Standard

Dunbar et al,53

1998Retrospective 2635 CSF

samples  Adults Positive CSF culture

Wasilauskas andHampton,54

1982

Retrospective 80 CSFsamples

  Adults Positive CSF cultureor bacterial antigen

Lannigan et al,55

1980

Cohort

(not clear if prospective)

434 16-86 y Positive CSF culture

Lindquist et al,56

1988Prospective

cohort710 2 mo but

majority adultsPositive CSF culture

or bacterial antigen

Briem,57 1983 Cohort(not clear if prospective)

266 90% 15 y or older Positive CSF cultureor bacterial antigen

Komorowskiet al,58 1986

Retrospective 562 Adults Positive CSF culture

 Abbreviation: CSF, cerebrospinal fluid.

Table 3. Accuracy of Cerebrospinal Gram Stain in Patients With Suspected BacterialMeningitis*

Study Sensitivity, %(95% CI) Specificity, %(95% CI)

LikelihoodRatio for

Positive Test(95% CI)

LikelihoodRatio for

Negative Test(95% CI)

Dunbar et al,53 1998 86 (74-92.6) 100 ( lower 95confidencelimit 99.7)

737 (230-2295) 0.14 (0.08-0.27)

Wasilauskas andHampton,54 1982

60 (47-71) . . . . . . . . .

Lannigan et al,55 1980 56 (34-75) . . . . . . . . .

 Abbreviation: CI, confidence interval.*Ellipses indicate that data are not available.

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were predictors of bacterial meningi-

tis. Severity was defined by the pres-ence of at least 1 of the following: al-tered consciousness, seizure, focalneurological findings, and shock. Be-cause this study was retrospective, rel-evant laboratory data was not avail-able for allpatients. And, this modelhasnot been prospectively validated in anindependent population.

How Should the Procedure

Be Performed?

Ideally, a successful LP shouldmeet the

following criteria: (1) obtain suffi-cient CSF on the first attempt, (2) oc-curs without trauma (ie, CSF contain-ing 1000 red blood cells per highpowered field), (3) occurs with mini-mal discomfort to the patient duringand after the procedure, and, (4) oc-curs without serious adverse eventssuch as cerebral herniation. The fol-lowing description of the method toperform an LP considers the best avail-able evidence and expert opinion to fa-cilitate successful LP completion.

The procedure and its risks shouldbe explained to the patient and in-formed consent obtained if relevant inthe practice setting. The descriptionshould include howthe procedure willbe performed, why it is being per-formed, what complications may oc-cur, and how these can be treated. Forexample, patients can be told that on

average, 6 out of 10 people may de-

velop a transient headache after LP andthat up to 4 out of 10 people can ex-perience temporary backache. Pa-tients should be asked if they are aller-gic to any medications including localanesthetic. For the anxious patient,some experts suggest that a small doseof anxiolytic (eg, lorazepam) may begiven prior to the procedure if the pa-tient wishes.

In the absence of any focal neuro-logical findings, altered mentation orpapilledema, the LP can be performed

without first completing a CT scan.12,13

If a CT scan is requested before the LPwhen bacterial meningitis is sus-pected, antibiotic therapy should bestarted immediately and should notawaitcompletion of the CT scan. If pos-sible, blood cultures should be takenprior to starting antibiotics.

The LP is usually completed with thepatient in the lateral recumbent posi-tion with his/her back at theedge of thebed to minimize curving of the spine(FIGURE 4). Both legs should be flexed

toward the chest and the neck shouldalso be slightly flexed. The patient’sshoulders and pelvis should be verti-calto thebed. In this position, an imagi-nary line connecting the patient’s pos-terior superior iliac crests would crossthe L4-L5 interspace (Figure 4). Lum-bar puncture can occur in the L3-L4,L4-L5, or L5-S1 interspace.67 It should

not be attempted at higher levels in or-der to avoid the conus medullaris. Thereis no evidence to guide the clinicianabout whether the L3-L4 or L4-L5 in-terspace is the optimal site for the ini-tial attempt, which is a topic high-

lighted for future research. Theperformance of LP may occur at L5-S1because there are fewer nerve roots anda relatively larger interspace. Thespinous process superior to the cho-sen interspace should be palpated. Theneedle should be inserted about 1 cminferior to the tip of this process.68

 Wearing sterile gloves and a mask,the clinician should cleanse the punc-ture site with an antiseptic solution byapplying it in a circular motion thatstarts at the center of where the punc-ture will occur. Sterile drapes can be

applied, leaving the puncture siteex po sed. Pal pate the i denti f i edspinous process again and with 2 to 3mL of local anesthetic (eg, lidocaine),infiltrate the patient’s skin and deepertissues allowing 1 to 2 minutes for thisto take effect. An atraumatic needledoes not have the same cutting edge asa standard needle so it may be prefer-able to use an introducer to puncturethe skin prior to insertion of theneedle if this needle type is used.Introduce the spinal needle (using the

same track that was used for the anes-thetic) and advance it horizontallywhile aiming toward the umbilicus toa depth of about 2 cm. If bone isencountered, withdraw the needle tothe subcutaneous position and reinsertat a slightly different angle. Continueto advance the needle until a pop isfelt, indicating penetration of the liga-mentum flavum. The needle shouldnow be in the subarachnoid space. When the stylet is withdrawn, clearfluid should drip. If no fluid emerges,

rotate the needle to ensure that no flapof dura is blocking flow of CSF. If there is still no fluid, reinsert the styletand advance the needle slightly, with-drawing the stylet after each move-ment. Pain radiating down either legindicates that the needle is too lateraland has touched nerve roots. If thisoccurs, immediately withdraw the

Table 4. Accuracy of Cerebrospinal Fluid Biochemical Analysis in Patients With SuspectedBacterial Meningitis

CSF Test

PositiveLikelihood

Ratio (95% CI)

NegativeLikelihood

Ratio (95% CI)

White blood cell count 500/µL56 15 (10-22) 0.30 (0.20-0.40)

Glucose 39.6 mg/dL (2.2 mmol/L)56 23 (13-40) 0.50 (0.40-0.60)

Blood glucose ratio 0.456 18 (12-27) 0.31 (0.21-0.45)

Blood glucose ratio 0.4Briem,57 1983* 145 (20.4- 1029) 0.25 (0.15- 0.40)

Lactate 27 mg/dL (3 mmol/L)Komorowski et al,58 1986 2.9 (2.4-3.5) 0.20 (0.06-0.50)

Lactate 31.5 mg/dL (3.5 mmol/L)Lannigan et al,55 1980 13 (8.6-20) 0.20 (0.06-0.50)

Lindquist et al,56 1988 25 (16-38) 0.12 (0.06-0.20)

Briem,57 1983† 38 (15-94) 0.01 (0.001-0.20)

Summary 21 (14-32) 0.12 (0.07-0.23)

 Abbreviations: CI, confidence interval; CSF, cerebrospinal fluid.*n = 245.†n = 218.

DIAGNOSING BACTERIAL MENINGITIS BY LUMBAR PUNCTURE

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needle almost to the skin, recheck thepatient’s position, and reinsert theneedle in the mid line. If this process

fails, move down 1 interspace and tryagain. If this fails, the procedureshould be attempted by another per-son; alternatively it can be done underfluoroscopic guidance. If the LPattempt is unsuccessful with thepatient in the lateral position, it maybe attempted with the patient sittingupright.50 However, this position doesnot permit accurate measurement of the CSF pressure.50,69

 WhenflowofCSFisseen,pressurecanbe measured by connecting a manom-

eter directly to the needle or via a 2- or3-way stopcock. The 0 mark on themanometer should be held at the levelofthespinalneedleandthetubeheldver-tically. Normally, therewillbe variationin the pressure with respiration.

The manometer contents can be re-leased for collection and analysis. Ad-ditional CSF can be collectedfor the re-quired investigations. Typically, theexaminer prepares 3 to 4 collectiontubes. The initial CSF sample is placedintoa tubelabeledfor biochemistry,and

tube number 2 is for bacterial studies(Gram stain, culture, and sensitivity).Cell counts can be done on tube num-ber 3, and the fourth tube can be usedfor cytology or for other tests that mightbe done when considering other diag-noses. These tests will vary dependingon the differential diagnosis for the in-dividual patient. Once CSF collection

has been completed, the stylet shouldbe reinserted prior to removal of theneedle. A bandage may be appliedto the

puncture site and the patient allowedto ambulate.

How Should This Procedure

Be Taught or Learned?

Recent surveys of clinical practice sug-gest variation in LP technique. A 1996survey of senior registrars in all depart-ments of neurology and neurosurgeryin the United Kingdom foundthat 15%of respondents reported using atrau-matic needles and that 73% recom-mended bed rest for up to 6 hours af-

ter completion of the LP.70

A similarsurvey of 2287 practicing neurolo-gistsin the United States found that 2%used atraumatic needles.70 Neurolo-gistsdidnot useatraumatic needles dueto lack of knowledge of them or be-cause the needles were not available foruse in their institution.71

The American Board of InternalMedicine recommends 3 to 5 LPs as aminimum standard for ensuring com-petence in completing LPs.72 How-ever, trainees report that they needed

to complete 6 to 10 procedures to feelcomfortable with their performance.72

Based on the evidence reviewed inthis article, some targets could be sug-gested to help clinicians assessthe qual-ity of their LP performance. A success-ful LP would be indicated by obtainingsufficient CSF fluid for analysis on thefirst attempt and by achieving risks of 

post-LP headache of less than 60% andof backache of less than 40%. How-ever, these figures represent the aver-

age risk of these events and thus can-not be considered as benchmarks.73

To date, there is little evidence toguide the teaching of this procedure.Simulators have been developed, butthey have not been rigorously evalu-ated.74 In one randomized trial of a Web-based educational tool, 14 nov-ice trainees were randomized to re-ceive Web-based training or no train-ing.75 The Web-based training moduleprovided virtual simulation of an LP.Both groups completed pre- and post-

LPs on a synthetic mannequin. Inves-tigators found an improvement in per-formance of LP with completion of thetraining module. There was no assess-ment of the procedural skills on pa-tients or of the risk of post-LP compli-cations. This evidence highlights theneed to evaluate teaching the perfor-mance of LP to ensure clinical compe-tence is achieved and maintained.

SCENARIO RESOLUTION

Our patient had a fever, confusion, and

neck stiffness. Many clinicians mightproceed immediately to LP to rule outmeningitis. However, we weren’t ableto complete a full neurological exami-nation because our patient was unableto cooperate with the assessment. Asdiscussed in this article, evidence sug-gests that her altered mental state andage and our inability to confirm theab-

Table 5. Studies That Developed and/or Validated Prediction Rules for Diagnosing Bacterial Meningitis*

Study Study DesignNo. of

Patients Age Model Accuracy

Spanos et al,60 1989 Retrospective chartreview

Derivationset, 120

1 mo AUC 0.97

Hoen et al,61 1995 Retrospective chartreview

500 1 mo AUC 0.99

Leblebicioglu et al,62

1996Retrospective chart

review40 NA Assessed model s by Hoen and Spanos AUC 0.95 by Spanos§

 AUC 0.99 by Hoen

McKinney et al,63 1994 Retrospective chartreview

170 17 y Assessed model by Spanos AUC 0.98

Baty et al,64 2000 Prospective 109 1-85 y Assessed model by Hoen Sensitivity 80%

 Abbreviations: AUC, area under the curve; CSF, cerebrospinal fluid; NA, not available; PABM, probability of acute bacterial meningitis; PMN, polymorphonuclear leukocytes.*Reference standard: bacterial meningitis is present when CSF, urine, or blood tests positive or when bacterial antigen is detected in blood, urine, or CSF.†Where L = 0.52 number of months from August 1 −12.76 CSF: blood glucose ratio (if ratio 0.6, use 0.6) 0.341 (PMNs in CSF 106 /1)0.333 2.29 age 2.79

(if age 1 y), −2.71 age 7.79 (if 1 y age 2 y), −0.159 age 2.69 (if 2 y age 22 y), or 0.1 age = 3.01 (if age 22 y).‡Where L = 32.13 10−4 CSF PMN count (106 /L) 2.365 CSF protein (g/L) 0.6143 blood glucose (mmol/L) 0.2086 leukocyte count (109 /L) −11.§Note the units used in the equation: glucose 1 mg/dL. To convert to mmol/L, multiply by 0.0555.Excluded those with encephalitis and in those for whom the diagnosis was obvious (eg, cloudy CSF or positive Gram stain).

1

(1 + e−L )PABM = †

1

(1 + e−L )PABM = ‡

DIAGNOSING BACTERIAL MENINGITIS BY LUMBAR PUNCTURE

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sence of a focal neurological finding areappropriate indications for a CT scanto rule out an intracranial lesion. Thissituation describes a common clinical

conundrum even though the inci-dence of intracranial lesions with suchpresentations is low. Because our clini-cal findings werestrongly suggestive of 

meningitis, we obtainedblood work in-cluding complete blood count, glu-cose, and cultures and initiated imme-diate antibiotic therapy. We decided to

Figure 4. Anatomical Considerations During Lumbar Puncture

SpinalCord

ConusMedullaris

CaudaEquina

Cauda Equina

CerebrospinalFluid

 Vertebral Body

L4

L3

L2

L1

L5

2        c     m      

Patient Position and Identification of Bony Landmarks A

Hand Position, Needle Direction, and Anatomical RelationshipsB

Line Between Superior Borderof the Posterior Iliac Crests

Intersecting L4 Spinous Process

Dura Mater

 Arachnoid

LigamentumFlavum

EpiduralSpace

Nerve Root

SupraspinousLigament(OverlyingSpinous Process)

InterspinousLigament

Lumbar PunctureNeedle

SpinousProcess

SubarachnoidSpace

T OP V I EW

Possible Needle Insertion Sites

L4L3 L5 S1

L4

1  c m 

 Vertical Alignment ofShoulders and Pelvis

  S  U  P  E  R

  I O  R

 A  N  

T   E  R  I  O R 

Lumbar puncture is usually performed with the patient in the lateral recumbent position. To avoid rotation of the vertebral column, align the patient’s shoulders andpelvis in a plane perpendicular to the bed. A line connecting the superior border of the posterior iliac crests intersects the L4 spinous process or the L4-L5 interspace.Insert the lumbar puncture needle in the midline of the L3-L4, L4-L5 (most commonly), or L5-S1 vertebral interspace. These interspaces are below the end of the spinalcord, which terminates at the level of L1. Angle the needle towards the patient’s umbilicus and advance it slowly. The needle will penetrate the ligamentum flavum,dura, and arachnoid to enter the subarachnoid space, where cerebrospinal fluid is located.

DIAGNOSING BACTERIAL MENINGITIS BY LUMBAR PUNCTURE

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obtaina CTscan (on which nomass le-sion was noted) before performing anLP. Given her confusion and uncer-tain neurological status, we suggestedambulation withassistance. We usedanatraumatic needle and completed the

procedure with the patient in the leftlateral recumbent position.Cerebrospi-nal fluid was sent for cell count, Gramstain, culture, protein, glucose, and lac-tate. Results showed a white blood cellcount of 5000/µL and a CSF–blood-glucose ratio of 0.2, helping us to di-agnose bacterial meningitis.

BOTTOM LINE

Lumbar punctures to assess meningi-tis in adults should be performed un-der sterile conditions with the patientplaced in the lateral recumbent posi-

tion and their knees flexed. Alterna-tively, the patient could sit up and leanforward with his/her feet supported toincrease the interspinous space. Formost patients, a CT scan of the head torule out mass lesion is not required be-fore the LP and the clinical examina-tion can guide the decision about neu-roimaging.

The following procedures may de-crease the risk of post-LP headache:

1. Use of small-gauge atraumaticneedles for diagnostic LPs is preferred

but may require more needle pass at-tempts,

2. Reinsertion of the stylet prior tothe removal of the spinal needle,

3. Mobilizationof patients after com-pleting the LP.

In patients suspected of having bac-terial meningitis, the following labora-tory tests can be considered along withappropriatecultures, Gram stain and se-rologic studies:

1. CSF–blood glucose ratio of 0.4 orless (LR positive, 18; 95% CI, 12-27; LR

negative, −0.31; 95% CI, 0.21-0.45).2. CSF white blood cell count of 500/µL or higher (LR positive, 15; 95%CI, 10-22; LR negative, 0.30; 95% CI,0.20-0.40).

3. CSF lactatelevel of 31.5 mg/dL orless (3.5 mmol/L; LR positive, 21;95% CI, 14-32; LR negative, 0.12; 0.07-0.23).

Author Contributions: Dr Straus had full access to allof the data in the study and takes responsibility for the integrity of the data and the accuracy of the dataanalysis. Study concept and design: Straus.Acquisition of data: Straus, Holroyd-Leduc.Analysis and interpretation of data: Straus, Thorpe,Holroyd-Leduc.Drafting of the manuscript: Straus.

Critical revision of the manuscript for important in-tellectual content: Straus, Thorpe, Holroyd-Leduc. Statistical analysis:Straus, Thorpe.Administrative, technical, or material support:Straus.Financial Disclosures: None reported.Funding/Support: Dr Straus is supported by a Tier 2Canada Research Chair; Mr Thorpe and Dr Holroyd-Leduc are supported by the Knowledge TranslationProgram at the University of Toronto.Role of the Sponsor: There was no external fundingobtained forthe designand conduct of thestudy, thecollection, management, analysis, or for the interpre-tation of the data; or for the preparation, review, or approval of the manuscript.Acknowledgment: We thank Darlyne Rath, LaurePerrier, and Desiree Chanderbhan for their assis-tance in the search and retrieval of relevant articlesandthe preparation of themanuscript as part of their normalduties. Richard Bedlack, MD,PhD, Division ofNeurology,and Sheri Keitz, MD,PhD, Divisionof Gen-eralInternalMedicine, DukeUniversity, Durham, NC,and Kaveh Shojania, MD, Department of Medicine,University of Ottawa, Ottawa, Ontario, who pro-vided useful feedback andsuggestions on earlier draftsofthe manuscript. We also thankJanisMiyasaki, MD,FRCPC, Department of Neurology, for comments onthe figure. They received no compensation for their reviews of the manuscript.

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