Ibrahim Shamia Pathological Specimenes

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OOPERATING PERATING RROOM OOM TTECHNIQUEECHNIQUE

:::PREPARED BY:::

Ibrahim H. Shamia ,RN, MSN, PhD Fellow

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Surgical SpecimensSurgical Specimens

All tissue removed during the surgical procedures is All tissue removed during the surgical procedures is sent to the pathology laboratory for verification of sent to the pathology laboratory for verification of the diagnosis.the diagnosis.

Tissue specimens may be stored in a refrigerator in Tissue specimens may be stored in a refrigerator in the laboratory or in some other location within the the laboratory or in some other location within the OR suite until they are taken to the pathology OR suite until they are taken to the pathology department at the end of each day’s schedule of department at the end of each day’s schedule of surgical procedures or intervals during the day.surgical procedures or intervals during the day.

Correct solutions for storage and correct labeling of Correct solutions for storage and correct labeling of specimens are critical for accurate diagnosis. specimens are critical for accurate diagnosis.

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Care of SpecimenCare of Specimen

- Types of Surgical Specimen:Types of Surgical Specimen:1. Pathological Specimen (Tissue).

2. Cultures.3. Smears & Fluids.

4. Stones.5. Foreign Body.

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Care of SpecimenCare of Specimen

1. Pathological Specimen

• This is a living tissue taken from the diseased part or a part from an organ.

The specimen should:1. Not be crushed.2. Not be lift dry.3. Put in a proper container to be

fully immersed in preservative solution. Usually formalin on a plastic container is used.

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Care of SpecimenCare of Specimen

Note:Note: • If frozen section biopsy is taken we

should not put it in solution but take it immediately to laboratory.

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Care of SpecimenCare of Specimen

2. Cultures:2. Cultures: Are obtained on sterile condition. Are obtained on sterile condition. Tips of swap must not be contaminated by any other Tips of swap must not be contaminated by any other

sources. sources. Should be refrigerated or sent to laboratory Should be refrigerated or sent to laboratory

immediately.immediately.

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Care of SpecimenCare of Specimen

3. Smear and Fluids:3. Smear and Fluids:Should be taken to laboratory immediately. These may Should be taken to laboratory immediately. These may be placed on glass slides or drawn in to evacuation tube.be placed on glass slides or drawn in to evacuation tube.Example: pus for gram stain or lymph node smears for Example: pus for gram stain or lymph node smears for electron microscope exam.electron microscope exam.

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Care of specimenCare of specimen

4. Stones:4. Stones:•Are placed in dry container Are placed in dry container so that they will not be so that they will not be dissolved.dissolved.

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Care of SpecimenCare of Specimen

Amputated Parts: Are sent to mortuary. It is warped in cover with identification. They are disposed as relatives wishes or

religions beliefs. A note containing description is sent to

pathology lab.

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Care of SpecimenCare of Specimen

--Specimen Container:Specimen Container:1- Plastic bags.

2- Waxed cardboard cartons.3- Glass jar with preservatives.

- - Each container is labeled Each container is labeled with:with:1.Name of patient.2. Hospital number or medical record.3. Room number.

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Care of SpecimenCare of Specimen

Laboratory Request Includes:1. Date of specimen taken.

2. Name of surgeon.

3. Pre- & post operative diagnosis.

4. Operative procedure.

5. Tissue to be examined.

6. Laboratory test the surgeon desires.