IFPAC 2008 1

C2V-200: a new Nessi compatible fast Micro GC

IFPAC, Baltimore 2008C2V BV, Enschede, The Netherlands

IFPAC 2008 2

Job Elders, PhD

CEO of C2V Ex GM for Alcatel Optronics NL with fiduciary responsibility Founder of TMP (Total Micro Products) Member of NanoNed Industrial Advisory Board & Invited referee of

Dutch Nanotechnology programme (€ 245M) Invited member of advisory board of Point One (€ 500M Philips,

ASML, Economic Affairs program) Member of program committee IOP precision technology Advisor of Technology Accelerator of University of Twente Co-chair of the international MANCEF MEMS Roadmap

(http://www.mancef.org) Board member of the international magazine “Micro Nano”, Executive board member and founder of MANCEF Member of the Management of Technology Advisory Board of the

Anderson school of Management, University of New Mexico Author of about 75 patents, technical and business papers PhD in Physical Chemistry, University of Amsterdam

Board member of Dosimetrix LLC Board member of foundation “Kokopelli”

IFPAC 2008 3

Key historical milestones

1991BBV founded

1995TMP foundedBBV Software launched

2000BBV and TMP acquired by Kymata

2001Kymata acquired by Alcatel. BBV and TMP integrated to form Alcatel Optronics NL

2002Management buy-out of AONL to form C2V

2003C2V re-aligned to market demand

2005C2V successfully pre-launches its Micro Gas Chromatograph

2006 Product introductionBeta site testingOEM product evaluation

2007Customer Shipments(Eng. Prototypes)

IFPAC 2008 4

C2V’s fast micro GC: enables Nessi compatibility

• 2000-2002: NeSSI Generation I– mechanical, passive components are on a SP76 substrate

• 2002-2004: NeSSI Generation II– “electrified” passive & active components are on a SP76

substrate– integral heating and temperature control of the substrate– all electrical components are integrated with SAM– sensors and actuators communicate via sensor bus– smart (self-diagnostic) sample systems

• 2005+: NeSSI Generation III– C2V-200: first high quality SP76 compatible microGC– C2V-200: micro analyzer adds value to NESSI platform

• 2007: Tipping point - First large scale commercial application:– Exxon together with Siemens implements Nessi compatible

sample conditioning for SPT (Singapore Parallel Train – chemical plant in Singapore)

IFPAC 2008 5

Fast micro GC vs. conventional GCTechnical Features / Benefits

Conventional GC(Macro drawbacks)

• Life-time• Reliability• Reproducibility• Calibration (s)• Quantitation (ppm)• Consumables

Fast micro GC Cartridge(Micro advantages)

• System loading x10<• IC derived manufacturing• IC-based tolerances, QA/QC• Highly controlled operating

parameters (temperature, flow, etc.)• Highly stable system performance• Longer life-time, lower flow rates,

Limited life-time

(6 months)

Extended life-time

(2-5 years)

smaller • faster • better • for less costs

IFPAC 2008 6

Conventional GC(Macro drawbacks)

• Requires skilled engineers• Requires trained service-men• Requires significant CapEx

investment

Fast micro GC Cartridge(Micro advantages)

• System pre-programmed• Exchange of cartridge by

laymen• Does not require significant

CapEx• Enables innovative revenue

model, from CapEx to OpEx budgets

Requires expert know howComplicates sales process

Simplifies usageSimplifies sales process

Fast micro GC versus conventional GCTechnical Features / Benefits

smaller • faster • better • for less costs

IFPAC 2008 7

C2V-200 micro GC

→ Fast analysis: 10-30 seconds→ Handheld sized→ Easy exchangeable GC channel cartridges → Low power system (< 15 W typical)→ Low costs (decrease per increasing volume)

→ Excellent separation: N=10’000-25’000→ Operating up to 180 oC → Fast T ramping: 100 oC / minute→ ppm sensitivity with TCD→ Less carrier/reference gas (operational 1 ml/min.)→ Diagnostics on chip: flow/pressure

C2V’s unique MEMS micro fluidics integration platform enables a world-class handheld chip-based GC instrument.

smaller • faster • better • for less costs

IFPAC 2008 8

C2V’s micro GC technology platform

Chip technology• Using micro technology to create low cost GC

components (valves, injector, detector, column, flow system, heating, flow diagnostics) with low internal volume and low thermal mass

Integration of chips• Using a fluidic multi chip module for

integration of these chip components in a fluidic system with low dead volume connections

Cartridge• The fluidic system module is assembled on an

exchangeable cartridge that contains the core GC functionality

smaller • faster • better • for less costs

IFPAC 2008 9

Micro Injector: Channel array chip Membrane valves Pneumatic actuation

GC Components: chips technology

Micro TCD Detector: ppm sensitivity ms response

Silicon columns: Fast heating Integration on-chip

Capillary columns: Commercial available Opt. geometry

smaller • faster • better • for less costs

IFPAC 2008 10

Fast GC Cartridge

C2V fast GC easy exchangeable cartridge (2” x 3.5” x 0.6”) contains:

C2V-200 micro GC module (3” x 4.5” x 1.2”) contains:

Injector and sample loop TCD detector Column Heating (2 zones)

Easy exchangeable cartridge Gas tight cartridge package Read-out & control electronics Pneumatics hardware Connectors (gas & data & power)

smaller • faster • better • for less costs

IFPAC 2008 11

Fast GC Cartridges

smaller • faster • better • for less costs

Cartridge Column Type ID

[mm]

Df

[µm]

Phase

Ratio

100% dimethylpolysiloxane 0.18 1 45.0

GCC1010 PLOT-U 0.25 8 7.81

GCC1020 WAS (PEG) 0.15 0.25 150

GCC1030 Molesieve 0.32 30 2.67

GCC1040 CarbonPLOT 0.32 3 26.7

Typical rentention time 15 seconds

Typical efficiency 10’000-15’000 plates

Typical flow rate 1-10 ml/minute

IFPAC 2008 12

Fast GC Cartridges

smaller • faster • better • for less costs

GCC1000 separation is almost entirely based on boiling points making this cartridge suitable for a wide range of applications. Typical components: alcohols, esters, flavors and aromas, free fatty acids, glycols, hydrocarbons, ketones, organic acids, oxygenates, PAHs, pesticides, polymers, steroids, solvents, sulfur compounds.

GCC1010 elutes polar compounds as perfectly symmetrical peaks, allowing them to be analyzed together with light hydrocarbons or permanent gases. Typical components: halogenated compounds, hydrocarbons C1-C6, H2S, ketones, oxygenated hydrocarbons, permanent gases/solvents.

GCC1020 provides optimal selectivity for many aqueous soluble compounds. Typical components: BTEX, methanol, alcohols, aromatic hydrocarbons, esters, FAME, flavors and aromas, free fatty acids, glycols, halogenated compounds, ketones, organic acids and solvents.

GCC1030 (molecular-sieve coated capillary column) is especially valuable when separating permanent gases. Typical gases separated with the GCC1030 include: He, H2, O2, CO, Ne, HD, N2, NO, Ar, D2, CH4, KrHT, X3, DT, CD4, Rn, T2.

GCC1040 separates N2, O2, CO, CO2 and C1-C2 hydrocarbons. Typical gases separated by the GCC1040 include: He, Xe, CO, Ne,CH4, CO2, O2/Ar, C2H6, N2, C2H4, Kr and C2H2

IFPAC 2008 13

C2V’s fast micro GC: data management

• C2V platform accommodates:– Fieldbus– Profibus– Modbus– Wireless

• And interfaces with:– RS 232– USB

smaller • faster • better • for less costs

IFPAC 2008 14

Natural gas analysis (up to C5)

VB1 column:→ 12 seconds @ 50 C → High separation quality→ C2-C5, neo-pentane visible

0

0.5

1

1.5

2

2.5

0 1 2 3 4 5 6 7 8 9 10 11 12 13

Time (Sec)

Sig

na

l

n-Hexanen-Pentane

iso-Pentane

neo-Pentane

n-Butane

iso-Butane

Propane

EthaneMethaneNitrogenCO2

• Injection time 10 msec• Column 50oC, chip 100oC• Pactuation = 3 bar • PHE=PNG = 1.5 bar• column: VB1 • column: 5m/0.18mm/1m

Poraplot column:→ 18 sec @ 80 C→ High separation quality→ N2, CH4, CO2, C2H6, C3H8

-0,5

0

0,5

1

1,5

2

2,5

3

3,5

0 5 10 15 20

Time (Sec)

Sig

na

l

Nitrogen

Methane

Carbondioxide

Ethane

Propane

Column: Poraplot U 5m × 0.25mm Carrier: Helium, 2 barColumn T: 80 degCInjection time: 10 ms

smaller • faster • better • for less costs

IFPAC 2008 15

Natural gas analysis: C3-C6

smaller • faster • better • for less costs

-0.3

0

0.3

0.6

0.9

1.2

1.5

1.8

2.1

2.4

2.7

3

3.3

3.6

3.9

4.2

4.5

4.8

0 5 10 15 20 25Time (sec)

Ou

tpu

t si

gn

al

Natural gas measurement

Column: VB-1, 5m/0.18mm/1μmSample loop: 5μLCarrier gas: Helium, 1 bar

Oven temperature: 550C

NitrogenCO2Methane Propane

n-Butane

neo-Pentane (500ppm)

iso-Butane

iso-Pentane (500ppm)

n-Pentane (500ppm)n-Hexane (500ppm)

Ethane

IFPAC 2008 16

Natural gas: Temperature Program Run

smaller • faster • better • for less costs

Signal TCD1

0

0,1

0,2

0,3

0,4

0,5

0,6

0,7

0,8

0,9

1

0 5 10 15 20 25 30 35 40time (sec)

ou

tpu

t (V

)

Signal TCD1

peak# 3Ethane

peak# 2Methane

peak# 4Propane

peak# 5iso-Butane

peak# 6n-Butane

peak# 7neo-Pentane

peak# 9n-Pentane

peak# 8iso-Pentane

peak# 10n-Hexane

peak# 11n-Heptane

peak# 12n-Octane

peak# 13n-Nonane

peak# 14Decane (11ppm)

Measurement date 1/4/2008Measurement time 11:43 AMInjector+detector T-setpoint (C) 120Column T-setpoint (C) 40-180Column ramp time (s) 40Injection time (ms) 100Measurement time (s) 45

IFPAC 2008 17

BTEX headspace measurement

0

0,2

0,4

0,6

0,8

1

2 4 6 8 10 12 14 16 18 20 22 24

Time (Sec)

Sig

na

l

BTEX headspace measurement

Column CP-Sil-8, 5m/0.2mm/0.33mHelium carrier, pressure 2bar

Oven temperature 80oCInjection time 200msInjected volume 5.5 l

air

benzene

toluene

m-xylenep-xylene

ethylbenzene

o-xylene

→ Very fast separation < 20 seconds (60oC)→ Excellent separation quality→ Zero peak tailing→ Headspace injection

5m CP-Sil-8, 60oC, 2 bar, 200 ms injection time

smaller • faster • better • for less costs

IFPAC 2008 18

Reproducibility

Reproducibility test: > 100,000 runs to date

Showing results of 5,000 consecutive runs

Demonstrates: stability and reliability: – Flow control– Temperature control– Injection repeatability

smaller • faster • better • for less costs

IFPAC 2008 19

Reproducibility

Retention time results: 5,000 consecutive runs

smaller • faster • better • for less costs

IFPAC 2008 20

Reproducibility

Peak height results: 5,000 consecutive runs

smaller • faster • better • for less costs

IFPAC 2008 21

Concept to Volume B.V.C2V

For further informationor to test a fast, miniature GC for evaluation

please contact us at miniGC@c2v.nl

Dr. J. Elders, CEO phone: +31 534 889 889

www.c2v.nl

Colosseum 20; 7521 PT EnschedeThe Netherlands

C2V-200: fast Micro GC