International Conference on

Biodiversity

Abs Soc Indon Biodiv vol. 3 | no. 7 |pp. 281‐312 | October 2016 

ISSN: 2407‐8069  

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SECRETARIAT ADDRESS1. Sekretariat Masyarakat Biodiversitas Indonesia, Kantor Jurnal Biodiversitas, Jurusan Biologi Gd. A, Lt. 1, FMIPA UNS,

Jl. Ir. Sutami 36A Surakarta 57126, Jawa Tengah, Indonesia. Tel. +62-897-6655-281. Email: biodiversitas@gmail.com.Website: biodiversitas.mipa.uns.ac.id/snmbi.html

2. International Conference on Biodiversity (ICB) 2016 Pontianak, International Affair Office of Universitas Tanjungpura.Rectorat Building 2nd Floor. Jl. Ahmad Yani, Pontianak 78124, West Kalimantan, Indonesia. Tel.: +62-561-739630, Fax.+62-561-739637

Organized by Selected manuscriptswill be available at

THIS PAGE INTENTIONALLY LEFT BLANK

TIME SCHEDULEInternational Conference on Biodiversity

Society for Indonesian Biodiversity (SIB)Pontianak, Indonesia, 8-9 October 2016

TIME ACTIVITIES PERSON IN CHARGE SITE

Oct 8, 2016

08.00-08.30 Registration Committee R108.30-08.40 Speech of the Committee Chairman of the committee R108.40-08.50 Opening speech Rector of Untan Pontianak R108.50-09.20 Performing Arts (Choir and Dance) Committee R109.20-09.30 Photo session and coffee break Committee R1, Lobby

09.30-11.00 Panel 1 Moderator R1Prof. Dr. Thamrin UsmanProf. Dr. Tsuyoshi Yoshimura

11.00-12.00 Parallel presentation IGroup 1: AO-01 to BO-01 Moderator R1Group 2: BO-02 to BO-09 Moderator R2Group 3: BO-10 to BO-17 Moderator R3Group 4: BO-18 to BO-25 Moderator R4Group 5: BO-26 to CO-06 Moderator R5

12.00-13.30 Rest, prayer, lunch & Poster session Committee Lobby

13.30-14.30 Panel 2 Moderator R1Prof. Dr. Susan E. Page

14.30-15.30 Parallel presentation IGroup 6: CO-07 to CO-13 Moderator R1Group 7: CO-14 to EO-01 Moderator R2Group 8: EO-02 to EO-08 Moderator R3Group 9: EO-09 to EO-15 Moderator R4Group 10: EO-16 to EO-23 Moderator R5

15.30-15.45 Coffee break, prayer Committee Lobby

15.45-16.00 Closing speech and other explanations Chairman of the committee R1

iv

TIME ACTIVITIES PERSON IN CHARGE SITE

Oct 9, 2016

07.00-07.30 Registration Committee Lobby07.30-08.00 Trip to “West Kalimantan Ecotourism” Committee -08.00-09.30 “West Kalimantan Ecotourism” Committee -09.30-10.00 Trip to airport Committee -10.00 At the airport Committee -

Upcoming events:1. Seminar Nasional Biodiversitas UNS, Solo, Indonesia, 4 November 2016

http://www.acb.uns.ac.id

2. SIB Congres & International Conference on Biodiversity, Solo, Indonesia, 4-6 November 2016http://biodiversitas.mipa.uns.ac.id/S/2016/solo/home.html

3. Seminar Nasional Masyarakat Biodiversitas Indonesia, Samarinda, Indonesia, 26 November 2016http://biodiversitas.mipa.uns.ac.id/S/2016/samarindakota/home.html

TABLE OF CONTENTSInternational Conference on Biodiversity

Society for Indonesian Biodiversity (SIB)Pontianak, Indonesia, 8-9 October 2016

CODE TITLE AUTHOR(S) PAGES

Genetic diversityAO-01 Morphological diversity and phylogenetic of kemenyan

(Styrax sp.) from North SumatraArida Susilowati, Cut RizlaniCholibrina, Muhtar A. Munthe,Henti Hendalastuti Rahmad

281

AO-02 Application of genetic markers mtDNA D-loop in theBorneo Orangutan (Pongo spp.) from West Kalimantan

Hari Prayogo, Achmad M.Thohari, Dedy D. Sholihin, LilikB. Prasetyo, Sugardjito

281

AO-03 Genetic diversity of Pandanus conoideus (Pandanaceae)based on AFLP

Lisye Iriana Zebua, TatikChikmawati

282

AO-04 The mitochondrial control region of catfish, Ompokhypophthalmus and Ompok eugeneiatus of RiauProvince, Indonesia

Roza Elvyra, Dewi IndriyaniRoslim, Della Rinarta, Tasiah

282

AO-05 Gaga chicken biodiversity of dangdut type and slow typefrom Sidrap (Sidenreng-Rappang), South Sulawesibased on bioacoustic, body weight, and collar bonelength

Abinawanto, Pipih SuningsihEffendi

282

AO-06 Identification of Growth Hormone (GH) Gene in SumbaOngole Cattle using Direct Sequencing

Paskah Partogi Agung, WidyaPintaka Bayu Putra, Saiful Anwar,Ari Sulistyo Wulandari, M.S.A.Zein, Syahruddin Said

282

AO-07 Superior character evaluation of East and NorthKalimantan local upland rice cultivars

Nurhasanah, Sadaruddin, WidiSunaryo

283

AP-01 Yield and Zn content of 22 IRRI and Indonesiaoriginated rice genotypes under Indonesian agroecosystem condition

Untung Susanto, Umi Barokah,Mallikarjuna Swamy

283

AP-02 Characterization of soybean genotype resistant to podshattering and performance of agronomic characters

M. Muchlish Adie, AydaKrisnawati

284

AP-03 Morphometric of gabus sentani fish, Oxyeleotrisheterodon (Weber 1907), from three villages at SentaniLake, Papua, Indonesia

Abinawanto, E. D. Sriyani, A.Laksono

284

vi

Diversity of speciesBO-01 Analysis the stomata number and chlorophyll level of

leaf plants in Arboretum Sylva Indonesia PC UntanPontianak, West Kalimantan

Desy Ayu Wulandari, EntinDaningsih, Reni Marlina

284

BO-02 Isolation of resistant mercure bacteria in illegal goldmining area of Kulor, East Singkawang, West Klimantan

Yudi Kurniawan, Entin Daningsih,Laili Fitri Yeni

285

BO-03 Sumatran camphor (Dryobalanops aromatic): Whereand How many remain?

Cut Rizlani Kholibrina, Aswandi,Arida Susilowati

285

BO-04 Evaluation of water quality using macroinvertebratebioindicator in Lahumoko River, North Buton District,Southeast Sulawesi

Kahirun, La Ode M. HarjoniKilowasid, Laode Sabaruddin,Mukhtar

285

BO-05 Utilization of some of rattan jernang species on the WestCoast of Aceh, Indonesia

Aswandi, Cut Rizlani Kholibrina,Arida Susilowati, Asep Sukmana

286

BO-06 Comparative morphology of pasak bumi (Eurycomalongifolia) in Gunung Ambawang and Bukit BenuahWest Kalimantan Indonesia

Siti Masitoh Kartikawati,Wahdina, Muflihati

286

BO-07 Diversity of Angiosperm as medicinal plant in Garuvillage, Landak District, West Kalimantan

Eka Ariyati, Syarifah Marlina,Ruqiah Panjaitan

286

BO-08 Regeneration and distribution patterns of daun sang(Johannesteijsmannia altifrons) at Sei Betung, GunungLeuser National Park, Sumatra

Kansih Sri Hartini, Hadi SAlikodra, Herman Mawengkang,Retno Widhiastuti

287

BO-09 Biodiversity of trees in the secondary old forest atSadaniang Sub District, Mempawah District, WestKalimantan

Ratna Herawatiningsih 287

BO-10 Plant diversity of understory species in the LoksadoProtected Forest, South Kalimantan

Sasi Gendro Sari 288

BO-11 Habitat of Northwest Bornean Orangutan (Pongopygmaeus pygmaeus) in Danau Sentarum National Park,Kapuas Hulu District, West Kalimantan

Albertus Tjiu, Dwi Astiani,Burhanuddin, S. Suci UtamiAtmoko

288

BO-12 Linnaeus on swamp forest of Kapuas Hulu District, Yuliati Indrayani, Dwi Astiani,Wiwik Ekyastuti

288

BO-13 Effect of rice seeds coated with biological agents toquality of seeds, and survival of Xoo and the biologicalagents in the seeds

Tantri Palupi, Satriyas Ilyas,Muhammad Machmud, EnyWidajati

288

BO-14 Preliminary evaluation of antitermitic activities of woodvinegar from oil palm trunk

H.A. Oramahi, TsuyoshiYoshimura, Farah Diba, Nurhaida

289

BO-15 Species diversity of macrofungi around Riam Engkuli inPancur Aji tourism area, West Kalimantan

Hanna Artuti Ekamawanti, RatnaHerawatiningsih, Nila Widyasari

289

BO-16 Diversity of medicinal plant in Mandor, Pontianak, WestKalimantan for intructional media in the shools

Reni Marlina, Ernita, Syamswisna 290

BO-17 Sweet corn yield and nutritive value of stover in threegrowing season with manure inoculated bybiodecomposer

Dwi Retno Lukiwati, Endang DwiPurbajanti, Retno IswarinPujaningsih

290

BO-18 Impacts of timber harvest on Hymenoptera biodiversityin a natural production forest in East Kalimantan,Indonesia

Ahmad Budiaman, Noor FarikhahHaneda, Ima Duwi Robaikah

290

BO-19 New record of wild banana (Musa balbisiana) fromWest Kalimantan

Ari Sunandar 290

vii

BO-20 Quick assessment method for calculation softshell turtle(Amyda cartilaginea) and reticulated python (Pythonreticulatus Schneider, 1801)

Mariana Takandjandji, HendraGunawan, Vivin S. Sihombing

291

BO-21 The spatial change of the tropical lowland forest and itspossible effect on biodiversity changes in SouthSumatra, Indonesia

Zulfikhar, Zulkifli H., Kadir S.,Iskandar I.

291

BO-22 Mononchid and dorylaimid nematodes from BanuaBotanic Garden, South Kalimantan

Auliya Hanifa Permata, AbdulGafur

291

BO-23 First records of species of the genera CobbonchusAndrassy, 1958 and Mylonchulus Cobb, 1916(Nematoda: Mononchida) from South Kalimantan

Abdul Gafur 292

BO-24 Length–length and length–weight relationships of Indianmackerel Rastrelliger kanagurta (Cuvier, 1817) from theMayalibit Bay, Raja Ampat, West Papua, Indonesia

Dian Oktaviani, Setiya Triharyuni,Duto Nugroho

292

BO-25 The prospects of utilization for bungur wood bychemical component analysis

Hikma Yanti, Evy Wardenaar 292

BO-26 Habitat characteristics and distribution pattern ofunderstory with anticancer potential in Cibodas ResortArea, Mount Gede Pangrango National Park, Indonesia

Yanieta Arbiastutie,, Ris HadiPurwanto, Mae Sri Hartati, JokoMarsono

292

BO-27 Etlingera elatior (Jack) R. M. Smith: Uses,phytochemicals, and biological activities

Marina Silalahi 293

BP-01 Size distribution and biology aspect of the scallopedhammerhead shark (Shyrna lewini) Iin Tanjung Luar,East Lombok, Indonesia

Ria Faizah 293

BP-03 Inventory fish catch fishermen in the Village of PadangTikar, West Kalimantan

Eko Sri Wahyuni, Linda Riana 294

BP-04 Population study of freshwater crayfish, Cherax spp.,from Indonesian Papua based on morphometrictcharacters

Abinawanto, H. Hamidah, A.Laksono, R. Eprilurahman

294

BP-05 Morphometric characterization of mantis shrimps,Harpiosquilla harpax and Oratosquilla oratoriacollected from Pelabuhan Ratu waters, Indonesia

Abinawanto, D. Iftitah, W.Wardhana

294

Diversity of ecosystemCO-01 Forest type diversity on carbon stocks: Cases of recent

land cover conditions of tropical lowland, swamp, andpeatland forests in West Kalimantan

Dwi Astiani, Mujiman, Rafiastanto 294

CO-02 Sustainable utilization of forest resources in the WestKalimantan and Sarawak border region; Strategi foroptimal local workforce absorption

Nurul Bariyah 295

CO-03 Role of soil microbes in increasing plant growth ofJatropha curcas in soil contamibated mercury

Wiwik Ekyastuti, Hanna ArtutiEkamawanti

295

CO-04 Carbon sequestration of bakau (Rhizophora apiculata)tree planted in Kubu Raya District, West Kalimantan

Rinto Wiarta, Dwi Astiani, YuliatiIndrayani, Fairus Mulia

295

CO-05 Differentiation of soil organisms at different types ofpeatland in West Kalimantan

Rossie Wiedya Nusantara, AsripinAspan

296

CO-06 Ecological study of Nepenthes pitopangii; A rare andendemic flowering plant of Central Sulawesi, Indonesia

Ramadhanil Pitopang, IrmasariThaha

296

viii

CO-07 Adoption of agroforestry patterns on community forestin Nusapati Village, West Kalimantan, Indonesia

Emi Roslinda, Wiwik Ekyastuti,Siti Masitoh Kartikawati, Syarifah

297

CO-08 Sago palm as a potential crop for peat restoration inWest Kalimantan

Evi Gusmayanti, Gusti Z. Anshari,M. Pramulya, Sholahuddin, NoviraKusrini

297

CO-09 Effect of space planting and organic fertilizer on planttalas beneng (Xanthasoma undipes)

Kartina AM, Apriany Fatmawaty,Nuniek Hermita, Eltis PancaNingsih

297

CO-10 A study on the potential for ecosystems and resources ofmangrove forests in Batu Ampar, Kubu Raya District,West Kalimantan

Adi Pasah Kahar 298

CO-11 Growth quality of sandalwood (Santalum album) inforest and plantation of West Timor Island, East NusaTenggara

Yoseph Nahak Seran,, Sudarto,Luchman Hakim, EndangArisoesilaningsih

298

CO-12 Stand development in 14-year-old restoration forest on acoal mined site in Central Bengkulu, Indonesia

Hery Suhartoyo, Yanchi Filyadi,Wiryono, Ali Munawar

298

CO-13 Indirect value of mangrove ecosystems in CoastalDabong, Kubu Raya District, West Kalimantan

Endang Karlina, Vivin S.Sihombing

299

CO-14 The estimates of spawning potential ratio of threedemersal dominant species landed in Tegal, Central Java

Duto Nugroho,, Mufti P. Patria,Jatna Supriatna, Luky Adrianto

299

CO-15 Interferometry SAR (INSAR) data analysis for peatlandand land use monitoring; Case study in Kubu Raya,West Kalimantan

Yessy Arvelyna, Kazuyo Hirose,Gusti Anshari, Tomita Kunihiro,Hosomi Koichi, Romi Yanto

299

CO-16 The correlation between environmental factors withmangrove clam abundance in different seasons at SegaraAnakan Mangrove Forest, Central Java, Indonesia

M. Sofwan Anwari 300

EthnobiologyDO-01 Ethnobotany in Tasik Malaya Village, Padang Tikar

Island, Kubu Raya District, West Kalimantan, IndonesiaSiti Nur Anisah, Entin Daningsih,R. Marlina

300

DO-02 Study of ethnobotanical potency in Kalaena NatureReserve, South Sulawesi

Hadijah Azis Karim, Proyanto 300

DO-03 Mangrove conservation ethnobiological to overcomeabrasion in Mempawah District of West Kalimantan,Indonesia

Laili Fitria, Jumiati 301

DP-01 Ethnobotanical study of herbal medicine in SebuduhVillage, Kembayan District, West Kalimantan,Indonesia

Syamswisna, Yokhebed, HadyNugroho

301

DP-02 Isolation and identification of antiradical andantiphotooxidant compound of Aloe chinensis

Yohana S. Kusuma Dewi,Tranggono, Sri Rahajo, PujiHastuti

301

BioscienceEO-01 Hepatoprotective effect of sambiloto leaves

(Andrographis paniculata) extract againtsMalondialdehyde levels in liver tissues of paracetamol-induced wistar rat (Rattus novergicus)

Andriani, Guntur Suseno, Effiana 302

ix

EO-02 Comparison of apple’s nutrient content (Malussylvestris) from various sale place in Pontianak, WestKalimantan

Rita Aprillia, Entin Daningsih,Titin

302

EO-03 The analysis of nutrient and fiber content of banana(Musa paradisiaca) which were sold in Pontianak City,West Kalimantan

Rizka Hasanah, Entin Daningsih,Titin

302

EO-04 Proximate analyzes of Tebas Orange (Citrus nobilis var.microcarpa) and Sunkist Orange (C. sinensis) varietiesnavel and valencia in Pontianak, West Kalimantan

Sandra Welya, Entin Daningsih,Titin

303

EO-05 Nutrient content of melon fruit (Cucumis melo) whichwere sold in Pontianak City, West Kalimantan

Tina Septiyani, Entin Daningsih,Titin

303

EO-06 Field activities and active flying hours of the biologypollinator of Apis mellifera honeybees in coffeeplantation

Budiaman 303

EO-07 Physical properties of wood as material of handicraft inWest Kalimantan with fumigation method

Lolyta Sisillia, Farah Diba 304

EO-08 Characteristic of composite boards made from sagotrunk with treatment different time of dipping sago trunkand percentage of compatibilizer

Dina Setyawati, Farah Diba,Nurhaida

304

EO-09 The impact of wood fumigation on rubber wood quality Lolyta Sisillia, Dina Setiawati 305

EO-10 Water quality of the Pitap river, South Kalimantan Sasi Gendro Sari 305

EO-11 Regulatory effects of five medicinal plants used byDayak Uud Danum in West Kalimantan, Indonesia onthe delayed-type hypersensitivity and the inflammationof human colon epithelial cells

Yeni Mariani,, Fathul Yusro,,Yuko Konishi, Takahiro Taguchi,Akira Tominaga

305

EO-12 Effects of medicinal plants in West KalimantanIndonesia to prevent the damage of Human ColonEpithelial FPCK-1-1 Cells and regulate the levels ofblood glucose and triacylglycerol of db/db mice

Fathul Yusro,, Yeni Mariani,,Yuko Konishi, Takahiro Taguchi,Mari Tominaga, Satoshi Kubota,Akira Tominaga

306

EO-13 Carbon balance in oil palm plantation on peatland: Asimulation of system dynamic model for reducinggreenhouse gas emission

Evi Gusmayanti, Gusti Z. Anshari,M. Pramulya

306

EO-14 Dual interaction between Trichoderma (Local isolates)with Glomus sp. in Acacia mangium seedling

Rosa Suryantini, Reine SuciWulandari, Slamet Rifanjani

306

EO-15 Innovation mechanical and digital biomass carbon meterfor monitoring, reporting and verification (MRV) forestcarbon in West Kalimantan

Gusti Hardiansyah, Yudha Arman,Faurizal, Sigit Normagiat

307

EO-16 The study of system and harvesting pattern of privateforest in Jeneberang Watershed, South Sulawesi,Indonesia

Iswara Gautama, DaudMalamassam, Budiaman

307

EO-17 Initial moisture content of corncobs plays an importantrole in maintaining its quality during storage

Lince Mukkun, Herianus J. D.Lalel, Yuliana Tandirubak

307

EO-18 Effectiveness of Trichoderma for control of FusariumWilt of Tomato

Agnes V. Simamora, Mayavira V.Hahuly

308

EO-19 Study of additional cassava maltodekstrin on Aloe verapowder making

Maherawati, Lucky Hartanti 308

EO-20 Lactic acid bacteria Sri Murwani, Indah Amalia Amri 308

EO-21 Land reclamation on coal mine by using FMA withintercropping systems in West Sumatra, Indonesia

Eti Farda Husin, Zelfi Zakir,Eddiwal

309

x

EO-22 Isolation of F protein New Castle Disease Virus of EastJava wild isolate

Sri Murwani, Dahliatul Qosimah,Indah Amalia Amri, Edy Sudjarwo

309

EO-23 Growth evaluation of two different types of grafting inthree superior varieties of Lai-Durian (Durio zibethinusx kutejensis) from East Kalimantan using differentrootstocks

Widi Sunaryo, Rahman, HadiPranoto

309

EP-01 Earthworm and ant for multiplication of micorrhizalspore

Laode Muhammad HarjoniKilowasid, La Aris, HasbullahSyaf, La Ode Santiaji Bande,Rachmawati Hasid, Bahrun,Halim, Syamsu Alam

310

EP-02 Acute toxicity study of the ethanolic extract ofEleutherine americana leaves in Wistar Rats UsingOECD Guideline 425

Robiyanto, Sri Wahdaningsih, EkaKartika Untari

310

EP-03 Formulation and methods of diagnostic kit storage instabilizer

Sayu Putu Yuni Paryati, EkaNoneng Nawangsih, Iis InayatiRakhmat

310

EP-04 Isolation and characterization of the antibody anti-idiotype N-terminal HPV 16 L2 from egg yolk

Eka Noneng Nawangsih, Sayu P.Yuni Paryati, Jusuf S. Effendy,Sunarjati Sudigdoadi, EdhyanaSahiratmadja

311

EP-05 Antifertility effect of Lerak () fruits ethanolic extract onfemale Sprague Dawley rats

Inarah Fajriaty, Hariyanto I.H.,Youngky Haryanto

311

EP-06 Identification antixenosis and antibiosis resistance ofsoybean genotypes to armyworm (Spodoptera litura)

Ayda Krisnawati, M. MuchlishAdie

311

EP-07 Effect of enzyme combination (polygalacturonase,cellulase and amylase) in keprok garut (Citrus nobilisvar. chrysocarpa) orange juice clarification

Esti Widowati, MAM. Riani,Cahyo Bagus Sulistyo

312

Note: A. Genetic Diversity, B. Diversity of Species, C. Diversity of Ecosystem, D. Ethnobiology, E. Bioscience (LifeScience and Technology); O. Oral, P. Poster

ABS SOC INDON BIODIVVol. 3, No. 7, October 2016 ISSN: 2407-8069Pages: 281-312 DOI: 10.13057/asnmbi/m030701

ABSTRACTInternational Conference on Biodiversity

Society for Indonesian Biodiversity (SIB)Pontianak, Indonesia, 8-9 October 2016

Genetic diversity

AO-01Morphological diversity and phylogenetic ofkemenyan (Styrax sp.) from North SumatraArida Susilowati1,♥, Cut Rizlani Cholibrina2, Muhtar A.Munthe1, Henti Hendalastuti Rahmad3

1Department of Forestry, Faculty of Agriculture, Universitas SumateraUtara. Jl. Tridharma Ujung No. 1, Kampus USU Medan. Tel./Fax.: +62-061-8220506. ♥email: arida_iswanto@yahoo.co.id2Research and Development of Environment and Forestry Agency of AekNauli. Jl Raya Parapat Km 10.5 Sibaganding Parapat, Simalungun 21174,North Sumatra, Indonesia.3Forest Research and Development Center, Ministry of Environmental andForestry. Jl. Gunung Batu No. 5. PO Box 165, Bogor 16001, West Java,Indonesia.

Kemenyan (Styrax spp.) is the most famous local treespecies from North Sumatera. Kemenyan is known as rosinproducer that is very valuable for pharmacheutical,cosmetic, food preservatives and vernis. Based on itshistory, there were only two species of kemenyan, namelykemenyan durame and toba, but in the process of naturaldistribution, we also found other type that are alleged asdifferent species. The objectives of this research were: (1).To get information about morphological diversity ofkemenyan in North Sumatera and (2). To get informationabout phylogeny clustering based on morphologicalcharacters. Data was collected from direct observation andmorphological characterization, based on purposivesampling technique at Phak phak Barat, North Sumatera. Ineach of the sub-districts, 10 samples were collected.Morphological characters were examined using descriptiveanalysis, phenotypic variability uses standard deviation,and cluster analysis uses NTSYS Spc 2.0.2 forexamination. Result showed that there were differencebetween 4 species of kemenyen (batak, minyak, durameand toba) based on the obsevation of 61 charactersincluding flower, fruits, leaf, stem, bark, crown type, woodand the resin. From NTSYS Spc 2.0.2 analysis and bothquantitative and qualitative characters examination of

kemenyan, they are clustered into two groups.Whereas,kemenyan minyak is separated from other cluster.

Diversity, kemenyan, morphology, phylogeny

AO-02Application of genetic markers mtDNA D-loop inthe Borneo Orangutan (Pongo spp.) from WestKalimantanHari Prayogo1, ♥, Achmad M. Thohari2, Dedy D.Sholihin3, Lilik B. Prasetyo2, Sugardjito4

1Faculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email: hpyogo@yahoo.com2 Faculty of Forestry, Institut Pertanian Bogor. Jl. Lingkar Akademik,Kampus IPB Darmaga, Bogor 16680, West Java, Indonesia 3 Departmenof Biology, Faculty of Mathematics and Natural Sciences, InstitutPertanian Bogor. Jl. Lingkar Akademik, Kampus IPB Darmaga, Bogor16680, West Java, Indonesia.4 Departmen of Biology, Universitas Nasional, Jl. Sawo Manila,RT.14/RW.3, Pasar Minggu, Jakarta Selatan 12520, Jakarta

West Kalimantan has two orangutan sub-species, namelyPongo pygmaeus pygmaeus and P. p . wurmbii which areseparated by a natural boundary, the Kapuas River. Landuse changes and hunting activities caused the Borneoorangutan population to decline significantly. Orangutanswere herded together to the area of oilpalm plantations orforest fragments. Many are captured, traded and kept bylocal communities. Captive orangutans which have beenseized by the authorities and placed in a rehabilitationcenter are to be reintroduced back into their natural habitat.Often the origin of confiscated orangutans is not known.The technology of molecular biology through a geneticapproach can help to determine the origin of theseconfiscated orangutans. The objective of this study was todetermine the genetic diversity of orangutan (Pongopygmaeus) kept in rehabilitation center in Sintang andKetapang in West Kalimantan using genetic markers D-loop. Sampling of blood and hair of orangutans wereinvestigated to find out their total DNA and continued withDNA amplification and sequencing processes. Two clusters

ABS SOC INDON BIODIV 3 (7): 281-312, Pontianak, 8-9 October 2016282

were discernible applied, each of which represented a sub-species of orangutans in West Kalimantan .

D-loop, genetic, orangutan, sub-species

AO-03Genetic diversity of Pandanus conoideus(Pandanaceae) based on AFLPLisye Iriana Zebua1,♥, Tatik Chikmawati2

1Department of Biology, Faculty of Mathematics and Natural Sciences,Universitas Cenderawasih. Jl. Kamp Wolker, Waena, Jayapura 99351,Papua, Indonesia. Tel./Fax. +62-967-572115, ♥email:lis_pandanus@yahoo.com2 Department of Biology, Faculty of Mathematic and Natural Sciences,Institut Pertanian Bogor. Jl. Raya Darmaga, Kampus IPB Darmaga, Bogor16680, West Java, Indonesia

Pandanus conoideus Lam. is a medical plant. It spreadsfrom Mollucas to Papua. Based on its fruit morphologicalcharacters, it is known about 36 variations in Papuan. Thisstudy was conducted in order to know the genetic diversityof pandanus conoideus originated from Papua using AFLPmarker. DNA application utilized primer combination ofPstI-11 with MseI-48, MseI-49, MseI-50. Leaf sample of15 cultivars were collected from 4 Papua region i.eManokwari, Serui, Jayapura, and Jayawijaya. Furthermore,genetic diversity relationship among the cultivars wereanalyzed by cluster and principal component analyses.Based on 46 polymorphic DNA band with the size of 50-350 bp, estimated value of the genetic diversity of the P.conoideus Lam. were diveded into four group consisting ofKenen, while the second group are Pery, Maler, Hibakaya,Arari, Maler-Kel, Watansine, Bargum, Yanggeru, Bargum-Kel, Pa, Sungkuar, and Hiwa. The third group is Wu andthe fourth group is Hibmena. Similarity coefficient rangedfrom 0.47 to 0.93. The lowest similarity coefficient (0.47)were found at Hibmena and Yanggeru cultivar, whereas thehighest coefficient were found on Yanggeru and Bargumcultivars. The results of polymorphic DNA band reductionshowed that 15 cultivars of the P.conoideus Lam. werespreaded in 5 groups through component one to componentfour by genetic diversity value that reaches 83, 12%.

AFLP, diversity, genetic, Pandanus conoideus

AO-04The mitochondrial control region of catfish,Ompok hypophthalmus and Ompok eugeneiatus ofRiau Province, IndonesiaRoza Elvyra♥, Dewi Indriyani Roslim, Della Rinarta,TasiahDepartment of Biology, Faculty of Mathematics and Natural Sciences,Universitas, Universitas Riau. Kampus Bina Widya Jl. H.R. SubrantasKM 12,5, Pekanbaru 28293, Riau. Tel. +62-761- 65593. email:roza.elvyra@lecturer.unri.ac.id

The objective of the research was to study themitochondrial control region of cafish, Ompokhypophthalmus and Ompok euegeniatus from RiauProvince. The mitochondrial control region universal isused in Polymerase Chain Reactions technique. The resultsof Polymerase Chain Reactions is partial control region 248bp. The sequence of Polymerase Chain Reactions, productof partial control region, was done by multiple alignmentwith other catfish from Genbank and then is analyzed byusing software of MEGA program version 6.0. The geneticdistance and the phylogenetic tree using Neighbor Joiningmethod based on sequence control region can be used todifferentiate Ompok hyophthalmus and Ompokeugeneiatus.

Control region, Ompok hypophthalmus, Ompok eugeneiatus, Riau

AO-05Gaga chicken biodiversity of dangdut type andslow type from Sidrap (Sidenreng-Rappang),South Sulawesi based on bioacoustic, body weight,and collar bone lengthAbinawanto1, ♥, Pipih Suningsih Effendi2

1Department of Biology, Faculty of Mathematics and Natural Sciences,Universitas Indonesia. Kampus UI Gedung E Lt. 2, Jl. Lingkar KampusRaya, Depok 16424, West Java, Indonesia. Tel.: +62-21-7270163 Fax. :+62-21-78849010. ♥email: abinawanto.ms@sci.ui.ac.id2 Faculty of Animal Husbandry, Institut Pertanian Bogor. Jl. Agatis,Kampus IPB Dramaga Bogor 16680, West Java, Indonesia

Gaga chicken which also called “ayam ketawa” is one ofthe local ornamental chickens originating from Sidrap(Sidenreng -Rappang), South Sulawesi. Gaga chickens hasunique crowing song, like human laughing. Gaga chickenwhich has long and fast crowing song is called dangduttype, while the short and slow crowing song chicken isnamed slow type. The objective of present study is toinvestigate the biodiversity of Gaga’s chicken based onbioacustic body weight, and collar bone length. Seventeensamples of slow type and five samples of dangdut typewere collected, respectively. The crowing sound(bioacustic data) was recorded and was analyzed by CoolEdit Pro Portable 2 software. The correlation betweenbioacustic data either with the collar bone length or withthe body weight were performed using SPSS (version 19).Long crowing of dangdut type was 3.43 sec. longer thanslow type (3.10 sec.). According to Pearson correlation test,there were positive correlation between bioacoustics eitherwith body weight or the collar bone length (r = 0.20).

Ayam ketawa, Gaga chicken, South Sulawesi

AO-06Identification of Growth Hormone (GH) Gene inSumba Ongole Cattle using Direct Sequencing

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Paskah Partogi Agung♥, Widya Pintaka Bayu Putra,Saiful Anwar, Ari Sulistyo Wulandari, M.S.A. Zein,Syahruddin SaidResearch Centre for Biotechnology, Indonesian Institute of Sciences.Cibinong Science Center, Jl. Raya Bogor Km 46 Cibinong-Bogor 16911,West Java, Indonesia. Tel. +62-21-8754587, Fax. +62-21-8754588,♥email: paskah_partogi@yahoo.com

The Sumba Ongole (SO) cattle is one of Indonesian localbreeds cattle. The existence of the SO cattle in Indonesiabegan since the Ongole cattle was imported from India in1914 and placed in Sumba island (East Nusa TenggaraProvince). These Ongole cattle well adapted in Indonesianclimate and became known as Sumba Ongole (SO) cattle.The SO cattle has excellent potential as beef cattleaccording to the ability to gain higher carcass percentagecompared to other local breeds cattle in Indonesia. Thegrowth hormone (GH) has a major impact on growth,lactation and mammary gland development in cattle. TheGH gene was a potential target for molecular variationstudy due to the importance of the GH gene function. Thisstudy was conducted to determine the sequence profile ofthe GH gene in the SO cattle using direct sequencingmethod. The sequence of the full length of GH gene wasconducted from 31 samples of the SO cattle. There are 23points mutation in the GH gene based on the sequenceanalysis. There are 16 points mutation that occurs in theintron region, 4 points mutation in the exon region, and 3points mutation in the 3’ flanking regions. The nucleotidesubstitutions are divided into transition (13 pointsmutation) and transversion (9 points mutation). There arethree synonymous nucleotide substitution occured in theGH gene i.e. (T>C) and (C>T) in the exon 2 and (C>A) inthe exon 5. Otherwise, the nucleotide substitution (C>G) inthe exon 5 caused amino acid change (LeuàVal). It can beconcluded that the GH gene in the SO cattle waspolymorphic and was different from the Bos indicus GHgene in the GenBank database.

Growth hormone, sequencing, Sumba Ongole

AO-07Superior character evaluation of East and NorthKalimantan local upland rice cultivarsNurhasanah♥, Sadaruddin, Widi SunaryoDepartment of Agroecotechnology, Faculty of Agriculture, UniversitasMulawarman. Jl. Pasir Balengkong No.1 Kampus Gunung Kelua,Samarinda, East Kalimantan-Indonesia 75119, Tel./Fax. +62-541-749159/738341, ♥email: nurhasanah_2710@yahoo.com

East and North Kalimantan have high rice geneticdiversities. More than three hundred local rice cultivarsreportedly exist in East and North Kalimantan, and they aredominated by upland rice cultivars. Unfortunately, most ofthe local rice cultivars were no well characterized andoptimally used in rice breeding programs. In this study, 146upland rice cultivars originated from East and NorthKalimantan were grown on the field for traitscharacterization. Several agronomical and morphological

traits were characterized in the population. The resultsshowed a large variation in agronomical characters such asplant height, culm number, culm diameter, leaf length, leafwidth, leaf angle, ligule length, panicle length, seeds widthand length, and weight of seeds. On the other hands, arelatively small divergence of morphological characterswas observed in the population. Several cultivars carriedpositive yield component characters such as tiller number,productive tiller number, and panicle length. Otherinteresting traits included into desired characters indeveloping new superior rice variety were also noticed.Based on that results, several suspected superior cultivarswere selected for further tested to fulfil the requirements ofvariety registration, and furthermore, could be proposed fornational/commercial superior upland rice varieties.

East and North Kalimantan, local rice, superior traits, traitscharacterization

AP-01Yield and Zn content of 22 IRRI and Indonesiaoriginated rice genotypes under Indonesian agroecosystem conditionUntung Susanto1,♥, Umi Barokah1, MallikarjunaSwamy2

1Indonesian Center for Rice Research. Jl. Raya 9 Sukamandi, Rancajaya,Subang 41256, West Java, Indonesia. Tel.: +62-260-520157, Fax.: +62-260-520158, email: untungsus2011@gmail.com2Plant Breeding Division, International Rice Research Institute, LosBanos, Laguna, The Philippines

One third of the world population is suffering Zndefficiency and sosio-economic losses of this problem istremendous. One strategy to overcome the problem is bydeveloping rice lines having high Zn content (Zn Rice).This research was aimed to test the yield and Zn content of22 rice genotypes originated from IRRI and Indonesia. Theexperiment was conducted in Cirebon district during WestSeason of 2013 under irrigated condition followed byrandomized complete block design of two replications.Twenty one days old seedlings were transplanted at 20 cmx 20 cm planting space of 2 m x 3 m plot size. Yield andZn content (XRF machine) were measured. The resultsshowed that BR28 (originated from Bangladesh; 8.86 t/ha)had higher yield compared to Ciherang (popularvariety;6.74 t/ha), while 10 lines had comparable yield withCiherang. Seven lines, i.e. IR69428-6-1-1-3-3 (35.68 ppm),PSBRc82 (34.48 ppm), IR91152AC-819 (34.22 ppm),IR83286-22-1-2-1-1 (33.08 ppm), IR64 (31.73 ppm),POKKALI (29.98 ppm), and IR83663-20-3-2-2 (29.90ppm) had higher Zn content compared to Ciherang (23.27ppm). IR91152AC-819 (5.08 t/ha), IR83286-22-1-2-1-1(5.75 t/ha), and IR83663-20-3-2-2 (5.69 t/ha) had higherZn content with comparable yield to Ciherang. These linesare prospective to be further tested and utilized.

Genotype, Indonesia, rice, yield, Zn content

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AP-02Characterization of soybean genotype resistant topod shattering and performance of agronomiccharactersM. Muchlish Adie♥, Ayda KrisnawatiIndonesia Legumes and Tuber Crop Research Institute (ILETRI). Jl. RayaKendalpayak Km 8, PO Box 66 Malang 65101, East Java, Indonesia. Tel.:+62-341-801468, 801075, Fax.: +62-341-801496, ♥email:mm_adie@yahoo.com

Pod shattering is one of the major obstacles on soybeancultivation in Indonesia’s tropical climate. A total of 30soybean genotypes, including variety of Anjasmoro (checkvariety for pod-shatter resistant) and variety of Grobogan(susceptible to pod shattering), were characterized for itsagronomic characters during the field research from June toSeptember 2015. The characterization for pod shatteringresistance was conducted in Laboratory of Breeding, Iletri,Malang (September – October 2015). The evaluation forpod shattering resistance using oven method, and thescoring rate follows IITA method (1986) withmodification. The characterization of 30 soybeangenotypes for its pod shattering resistance resulted 14resistant genotype, 10 moderate, 1 susceptible, and 5 verysusceptible to pod shattering. The determinant of resistantto pod shattering was pod wall thickness (r = -0.442*), thethicker of the pod wall thickness the more resistant to podshattering it will be. The longer of the soybean pod lengthcaused the pod wall gets thinner and susceptible to podshattering (r = 0.410*). Among 14 resistant genotypes, twoof them (G511H/Argom//Argom-2-1 and G511H/Anjasmoro-1-7) with shattering percentage of 2.50% and4.16%, respectively, were more resistant than Anjasmoro(5.0%). Genotype of G511H/Anjasmoro-1-7 has agronomiccharacters such as days to maturity is 79 days, large seedsize (14.93 g), and seed yield reaches 2.97 t/ha, andtherefore it is more suitable to be developed in the soybeanproduction center in Indonesia.

Agronomic characters, pod shattering, soybean, resistantdeterminant

AP-03Morphometric of gabus sentani fish, Oxyeleotrisheterodon (Weber 1907), from three villages atSentani Lake, Papua, IndonesiaAbinawanto♥, E. D. Sriyani, A. LaksonoDepartment of Biology, Faculty of Mathematics and Natural Sciences,Universitas Indonesia. Kampus UI Gedung E Lt. 2, Jl. Lingkar KampusRaya, Depok 16424, West Java, Indonesia. Tel.: +62-21-7270163 Fax. :+62-21-78849010. ♥email:abinawanto.ms@sci.ui.ac.id

Research on morphometric of gabus Sentani fish,Oxyeleotris heterodon (Weber 1907) has been performed.The aim of this study was to compare the morphometriccharacters of gabus fish that has been found in the Sentani

lake, based on 22 characters. The total of 56 samples werecollected, which consisted of 14 samples from the Ifarvillage, 17 samples from the Sosiri village and 25 samplesfrom Putali village, respectively. The results of this studyindicated that there were differences in morphometriccharacters of gabus Sentani at Lake Sentani. Therelationship of morphometric characters among the threevillages were ranging from very weak to very strong basedon the linear regression analysis. Growth status werepositive allometric, isometric and negative allometric,respectively.

Gabus Sentani fish, morphometric, Oxyeleotris heterodon

Diversity of species

BO-01Analysis the stomata number and chlorophyll levelof leaf plants in Arboretum Sylva Indonesia PCUntan Pontianak, West KalimantanDesy Ayu Wulandari♥, Entin Daningsih, Reni MarlinaProgram of Biology Education, Faculty of Teacher Training andEducation, Universitas Tanjungpura. Jl. Prof. Dr. H. Hadari Nawawi,Pontianak 78124, West Kalimantan, Indonesia. Tel./Fax.: +62-561-740144, ♥email: desy_ayu_wulandari@yahoo.com

This research aimed to determine the stomata number andchlorophyll level of leaf plants in Arboretum SylvaIndonesia PC Untan Pontianak, West Kalimantan. Theresearch was a descriptive study. The stomata number wasexamined through stomata impression method, whilstchlorophyll level was measured using chlorophyll meterSPAD. There are 34 species of plants as research samples.Results showed that the highest number of stomata wasPendara Putih (Knema sp.) from Myristicaceae family with2532 and the lowest was Damar (Agathis borneensisWarb.) from Araucariaceae family with 212. The highestlevel of chlorophyll (SPAD) was Bongang(Tabernaemontana macrocarpa Jack.) from Apocynaceaefamily with as much as 83, 10 and the lowest was Ekaliptus(Eucalyptus pellita F. Muell.) from Myrtaceae family withas much as 29, 77. Plants having potential to reduce carbondioxide were Pendara Putih (Knema sp.), Keput Bajuku(Stemonurus scorpiodes Becc.), Mahoni (Swieteniamahagoni L.), Putat (Baringtonia reticulate (Blume.) Miq.)and Tengkawang Hutan Padang (Shorea pachyphylla Ridl.ex Symington.). They could reduce carbon dioxide becausethey had high stomata number and chlorophyll level.

Chlorophyll level, Pontianak, stomata number

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BO-02Isolation of mercure resistant bacteria in illegalgold mining area of Kulor, East Singkawang, WestKlimantanYudi Kurniawan♥, Entin Daningsih, Laili Fitri YeniProgram of Biology Education, Faculty of Teacher Training andEducation, Universitas Tanjungpura. Jl. Prof. Dr. H. Hadari Nawawi,Pontianak 78124, West Kalimantan, Indonesia. Tel./Fax.: +62-561-740144, ♥email: yudikurniawan691@gmail.com

Illegal gold mining in Kulor, Panjitan village, EastSingkawang caused damage to most of the vegetation andthe environment. They became wasted and polluted bymercury. One of the efforts to restore the environmentdamage was by using the help of mercury resistant bacteriaas agents of bioremediator. This study aimed to explore thetypes of mercury resistant bacteria. Bacteria were isolatedfrom sediment in illegal gold mining Kulor, EastSingkawang. The characters of bacteria includemorphology (colony and cell), physiological (motility,oxidase and catalase tests), and biochemical (carbohydratefermentation, indole, decarboxylation, Simmon citrate, andurease test). The mercury resistance test on Bacteria wasusing paper disc method. Twenty bacteria weresuccessfully isolated, of which 11 were mercury resistantbacteria. It could be seen from a clear zone around thepaper disk. Characterization results showed similarcharacteristics of mercury resistant bacteria that wereisolated with the genus of Alcaligenes, Enterobacter, andBacillus.

Bacteria, mercury resistance, gold mining

BO-03Sumatran camphor (Dryobalanops aromatic):Where and How many remain?Cut Rizlani Kholibrina 1,♥, Aswandi 1, Arida Susilowati2

1Research and Development of Environment and Forestry Agency of AekNauli. Jl Raya Parapat Km 10.5 Sibaganding Parapat, Simalungun 21174,North Sumatra, Indonesia. ♥email: rizlanicut@gmail.com2Department of Forestry, Faculty of Agriculture, Universitas SumateraUtara, Jl. Tridarma Ujung No. 1, Kampus USU, Medan 20155, NorthSumatra, Indonesia

Unsustainable forest management, encroachment and forestconversion drastically reduced the Sumatran champor treespopulation (Dryobalanops aromatica) on their habitat. Thisstudy aims to present the latest information aboutdistribution of Sumatran champor trees on western coast ofSumatra. The exploration was conducted on their naturalpopulation in Sirandorung Barus, Central Tapanuli District,Province of North Sumatra and Kadabuhan and SultanDaulat in Subulussalam and Singkohor in Aceh SingkilDistrict, Province of Aceh in May 2015 until July 2016.The results showed that the trees are only found in small-fragments of remaining forests on the ridges and valleys

with limited accessibility, from coastal region to 700meters above sea level. The largest average of treedimensions and the highest stand density found inKadabuhan. The trees diameter range 85-180 cm withnumber of population reaches 100-200 trees/ha. In threeother locations, trees with smaller dimension grow aroundoil palm plantations, fragmented and mixed with othertrees. Champor harvesting by splitting the timber hasimpacted on the population. Another means are bycollecting crystals out from trunk pores or distilling theleaves and branches. From one fallen tree, it was obtained1.5-2.5 kg of champor crystals and 5-10 kg of oil (ombil).However, at this time the potential NTFP commodity isdifficult to obtain and the trees were included in list ofcritically endangered species.

Critically endangered species, distribution, Dryobalanopsaromatica, natural population, Sumatran champor

BO-04Evaluation of water quality usingmacroinvertebrate bioindicator in LahumokoRiver, North Buton District, Southeast SulawesiKahirun1,♥, La Ode M. Harjoni Kilowasid2, LaodeSabaruddin3, Mukhtar4

1Department of Enviromental Science, Faculty of Forestry andEnviromental Science, Universitas Halu Oleo. Jl. HEA Mokodompit,Anduonohu, Kendari, Southeast Sulawesi, Indonesia2Biodiversity Laboratory, Department of Agrotechnology, Faculty ofAgriculture, Universitas Halu Oleo. Jl. HEA Mokodompit, Anduonohu,Kendari, Southeast Sulawesi, Indonesia. Tel.: +62-401-3191692, Fax:+62-401-3191692, ♥email: lohardjoni2@yahoo.co.id3Department of Agrotechnology, Faculty of Agricultural, Universitas HaluOleo. Jl. HEA Mokodompit, Anduonohu, Kendari, Southeast Sulawesi,Indonesia4Department of Agribusines, Faculty of Agricultural, Universitas HaluOleo. Jl. HEA Mokodompit, Anduonohu, Kendari, Southeast Sulawesi,Indonesia

The purpose of this study is to assess the water quality ofthe Lahumoko river by using macroinvertebrate as abioindikator and do an assessment of river habitat todetermine the level of water quality and the health of theriver. Observations in biology using the analysis of theFamily Biotic Index (FBI) for the existence ofmacroinvertebrate and river habitat assessment todetermine the health of the habitat, using a scoring systemwith the set index to determine the health of the riverecosystem of Lahumoko. Biota sampling was done at thefive sampling stations that were considered purposive andevaluated land use in the border river which represents theupper, middle and lower reaches of the LahumokoWatersheed. Three of five stations i.e. station LM 1, LM2and LM 3 were in the upper watersheed and the use of itsland was for forestry. Another station was in the centralpart of watersheed i.e. LM 4 station with land use isdominated by mixed orchards and one other station was inthe lower reaches of the watersheed i.e. LM 5 station withits land use is residential and mixed orchards. Biotasampling was done by handnet on the right, left and middle

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of the river. Examples of biota were identified to familylevel. The results of this research show that the total of theindividual that was found in the river Lahumoko is 356consisting of 13 individuals in the family. The distributionof family and individuals of macroinvertebrate tends todecline at LM 4 with farm garden mix land use and at LM5 station with residential land use. The results ofBiomonitoring of macroivertebrate and the assessment ofriver habitat concluded that the water quality of the riverLahumoko is fairly well in the degree of pollution with abit much, and healthy habitat conditions, providing habitatfor a varied enough and stable enough to support biota life(on station LM1, LM2, and LM 3) but on station LM4 andLM 5, the water quality is rather poor in the level ofpollution, and so it is an unhealthy habitat conditions, thehabitats are not varied and unstable to support biodiversityand life. So it can be concluded that the condition ofLahumoko Watersheed in general can be said alreadydegraded for its natural resources and environment is a bitheavy and it has pressure from the community in utilizingnatural resources which are not environmentally friendlyand pay no attention to the conservation norms.

Bioindicators, Family Biotic Index, macroinvertebrates, waterquality, watersheed

BO-05Utilization of some of rattan jernang species on theWest Coast of Aceh, IndonesiaAswandi1, ♥, Cut Rizlani Kholibrina 1, Arida Susilowati2, Asep Sukmana 1

1Research and Development of Environment and Forestry Agency of AekNauli. Jl Raya Parapat Km 10.5 Sibaganding Parapat, Simalungun 21174,North Sumatra, Indonesia. ♥email: andiasw@mail.ugm.ac.id2Department of Forestry, Faculty of Agriculture, Universitas SumateraUtara, Jl. Tridarma Ujung No. 1, Kampus USU, Medan 20155, NorthSumatra

One of non-timber forest products management with a longhistory in west coast of Aceh is a collection of dragon'sblood resin from some rattan jernang species. The resincontains important phytochemical compounds aspharmaceutical raw materials, cosmetics, preservatives andorganic dyes. The objective of study is to describe theutilization of some rattan jernang species in west coast ofAceh. The exploration was carried out in 2015 on WestAceh, Nagan Raya and South Aceh district, Province ofAceh. There are three rattan jernang species that harvestedby local community i.e. Daemonorops draco, D. crinitus,and D. angustifolia. The rattans grow on 150-2000 metersabove sea level in the Leuser Ecosystem. Among these,resin membranes of D. angustifolia fruits are thicker thusthey produce higher resin rendement (4-5%) for superquality. It begins to produce fruits at the age of 3 andcontinues until 20 years old. The harvesting is carried outwhen the fruits are still young and many reddish fine grainsresin bounded to fruit’s skin. Every two weeks, the mainharvest was done on August and January. The weight offresh young fruit reaches 300-500 g each bunch and an

individual mature rattan produce fresh fruit of 10-15kg/tree/year. Most of these are processed to obtain theresins which are marketed to Medan, North Sumatra andsubsequently forwarded to Jakarta, Jambi, Surabaya, orexported to Singapore, Malaysia and China. However, atthis time the resin productivity is decreasing due to habitatdestruction. Low of natural regeneration ability causes thepopulation decline. Also, the ripe fruit is difficult to obtainas a consequence of young fruit harvesting, so that plantseed preparation is limited. The harvesting practice bycutting the trunk is also one of the causes of their naturalpopulation reduction.

Community forestry, Daemonorops, dragon’s blood resin, NTFPs,west coast Aceh

BO-06Comparative morphology of pasak bumi(Eurycoma longifolia) in Gunung Ambawang andBukit Benuah West Kalimantan IndonesiaSiti Masitoh Kartikawati♥, Wahdina, MuflihatiFaculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email: kartikawt@gmail.com

Pasak bumi (Eurycoma longifolia Jack) is a medicinalplants found in Malay and western Indonesia, includingKalimantan island. People in West Kalimantan know onlyone kind of pasak bumi, named Eurycoma longifolia Jack.But based on previous researches, pasak bumi in WestKalimantan morphologically shows different leafcharacters in different habitat. This research aims to findout morphology variation on pasak bumi in Hutan LindungGunung Ambawang-Pemancingan compared to the onefound in Bukit Benuah, Kabupaten Kubu Raya WestKalimantan, Indonesia, which location is known as a goodhabitat for pasak bumi in West Kalimantan. Five plantsamples from both location was dated and measured formorphological characters, and the plant speciments wassend to LIPI Herbarium Bogoriense for furtheridentification. Comparative morphological study showsthat there were significant differences in leaf shape andsize, leaf number, and a few other morphologicalcharacters, but the plant speciment from both location wasidentified as the same species, i.e. Eurycoma longifoliaJack.

Eurycoma longifolia, habitat, morphology, pasak bumi, WestKalimantan

BO-07Diversity of Angiosperm as medicinal plant inGaru village, Landak District, West KalimantanEka Ariyati♥, Syarifah Marlina, Ruqiah Panjaitan

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Program of Biology Education, Faculty of Teacher Training andEducation, Universitas Tanjungpura. Jl. Prof. Dr. H. Hadari Nawawi,Pontianak 78124, West Kalimantan, Indonesia. Tel./Fax.: +62-561-740144, ♥email: eka.ariyati@fkip.untan.ac.id

Tendency of public awareness to go back to nature byusing traditional medicine derived from surrounding plantsresulted in medicinal plants being sought by the people thatmay lead to exploitation. This research aims to find out thediversity of Angiospermae used as a medicinal plants inGaru Village, Landak Regency. The method used in thisresearch was survey, observation, and identification.Identification is done through the book from Steenis,Wijayakusuma, and Heyne. Data presented in tables andanalyzed descriptively. Observation and indentificationfrom four locations i.e. yards, fields, forests, and roadsidesfound 40 plant species of Angiospermae that havemedicinal properties comprising families of Acanthaceae,Annonaceae, Asteraceae, Caesalpiniaceae, Compositae,Convolvulaceae, Dilleniaceae, Euphorbiceae, Fabaceae,Goodeniaceae, Gramineae, Hypoxidaceae, Labiatae,Lamiaceae, Malvaceae, Menispermaeceae, Moraceae,Myrtaceae, Phylanthaceae, Piperaceae, Orchidaceae,Rubiaceae, Rutaceae, Solanaceae, Thymelaceae,Umbelliferae, Vitaceae, Xanthorrhoeaceae, andZingiberaceae. It can be concluded that the species ofmedicinal plants found in Garu Village are 40 species andare dominated by the families of Zingiberaceae.

Angiospermae, diversity, medicinal plant

BO-08Regeneration and distribution patterns of daunsang (Johannesteijsmannia altifrons) at Sei Betung,Gunung Leuser National Park, SumatraKansih Sri Hartini1,♥, Hadi S Alikodra2, HermanMawengkang3, Retno Widhiastuti3

1Department of Forestry, Faculty of Agriculture, Universitas SumateraUtara. Jl. Tridarma Ujung No. 1, Kampus USU, Medan 20155, NorthSumatra, Indonesia. ♥email: ksudjarwo@gmail.com2 Faculty of Forestry, Institut Pertanian Bogor. Jl. Agatis, Kampus IPBDramaga Bogor 16680, West Java, Indonesia3 Faculty of Mathematics and Sciences, Universitas Sumatera Utara. Jl.Tridarma Ujung No. 1, Kampus USU, Medan 20155, North Sumatra,Indonesia

Conservation status of Daun Sang (Johannesteijsmanniaaltifrons) in Indonesia is in vulnerable category (VUD2).One indication of this category is the species will be at highrisk of extinction in the near future, in the criteria of asmall population and limited. The current existing status ofDaun Sang needs to be found out. This research aims toanalyze the level of regeneration and how the distributionpatterns of each level of regeneration. The level ofregeneration is an indication of sustainability, while thedistribution pattern indicates the division of space andcompetition between regeneration level. The methode usedwas purpossive sampling with single plot. There was 30plots, each size is 20 x 20 m. Observation was done onDaun Sang in each plot. It was to record: the number of

Daun Sang, crown diameter, height, number of leaves,number of live leaves, length and widht of leaves (length ofmidribs, length of leaves, width of the base and the middleof the leaves). Data was classified using clasify-K-Meansof SPSS software. Multi linear regression analysis wasdone to view the relationship between factors used todivide the regeneration level of Daun Sang. Distributionpatterns of Daun Sang was analyzed with Morisita Index(MI). The results showed that the level of regenerationdivided into seedling, juvenile and adult, and thepopulation of Daun Sang tends to decrease, while thedistribution patterns for various levels of regeneration israndom. Under these conditions, conservation effort ofDaun Sang needs to be done immediately in order tomaintain its existence

Conservation, Daun Sang, distribution pattern, regeneration

BO-09Biodiversity of trees in the secondary old forest atSadaniang Sub District, Mempawah District, WestKalimantanRatna Herawatiningsih♥

1Faculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email:rherawatiningsih@yahoo.co.id

No longer primary forest logging has been done at aroundthe year 1980-1990 at Mempawah District, WestKalimantan. Primary forest has already grown again andhas become secondary forest. In 2016, the forest has growninto old secondary forest that must always be preserved. Inthe sub district Sadaniang, Mempawah District, there canstill be found an old secondary forests represent one type oflowland forest. The purpose of this study is to determinethe biodiversity of tree species in the old secondary forestarea at the Sadaniang Sub District, Mempawah Sistrict. Themethod of this study is a survey method by cruisinginventory to track and plot with 900 meters length and 20meters width from the left lane to the right lane. From thetotal area of approximately 427.41 hectares, several kindsof area can be found such as oil palm plantations, rubberplantations, land burnt, old secondary forest land. In the oldsecondary forest, it can be found about 80 species with17.446 trees and its volume is approximately 2.3817.05m3. From the biodiversity of tree species, it can be foundMeranti tree species such as Meranti group of white, RedM., M. Stone, M. Eagles, M. Flowers, Tengkawang,Keruing, Resak, Balau, Bengkayat, Tekam ; and it is alsodiscovered a class of tree jungle mix as Bintangor,Keladan, Kempas, Nyatoh, Laban, Ubah, Keminting,Kubita, Keranji, Ketiau, Tamau, Pulai, Mentibu, etc. Later,it has discovered a class of timber trees as beautiful asAgathis, Rengas, Ramin, Wood night, Empening,Barangan, and Ribu-Ribu. For the potential of various treespecies, it is found about 4.364 Meranti tree trunk groupswith wood volume of 5335.98 m3; 10 976 of mix trees

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forest with 15.289 m3 of timber volume; and 2, 106 oftrees with beautiful wood with volume wood of 3129.69m3.

Biodiversity, old secondary forest, tree

BO-10Plant diversity of understory species in theLoksado Protected Forest, South KalimantanSasi Gendro Sari♥

Department of Biology, Faculty of Mathematics and Natural Sciences,Universitas Lambung Mangkurat. Jl. A. Yani Km. 36, Banjarbaru 70714,Kalimantan Selatan, email: sgsari@unlam.ac.id

Studying plant diversity of understory species in theLoksado Protected Forest is important to identify and tocharacterize all understory species. The aim of this researchwas to measure plant diversity of understory species insidethe Loksado Protected Forest. Vegetation analysis wasmade using a transect method taken from the edge up intoinside the forest at a range of ± 1 km. A continuous plotfrom 0 m, 250 m, 500 m, 750 m and 1, 000 m was usedwith a size of 10 m x 10 m and the identification ofunderstory species was done by LIPI Bogor. The resultshows that it can identify 46 species with diversity indexfrom 2.12 to 2.4 categorized as mediate. Diversity of thesespecies increased as it went deeper into the forest.Pronephrium nitidum Holttum or the local name“Singgagai” had the highest percentage of Important ValueIndex in five continuous plots and presented thecharacterization of understory species in the LoksadoProtected Forest.

Diversity, Pronephrium nitidum, understory species

BO-11Habitat of Northwest Bornean Orangutan (Pongopygmaeus pygmaeus) in Danau Sentarum NationalPark, Kapuas Hulu District, West KalimantanAlbertus Tjiu♥, Dwi Astiani, Burhanuddin, S. SuciUtami Atmoko♥♥

Faculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email: albertus@wwf.id

The Bornean Orangutan sub-species Pongo pygmaeuspygmaeus has the most minimal information compared totwo others sub-species in Kalimantan (Pongo pygmaeuswurmbii and Pongo pygmaeus morio). There is also lack ofdata for most behavior and ecology of this subspeciesbecause no long-term field research has been conducted.Research was done in Danau Sentarum National Park(BKNP) in Kapuas Hulu District. The purpose of this studywas to investigate the Orangutan habitat quality(P.p.pygmaeus) through assessing the forest composition

and diversity of vascular plant in swamp forest and theircorrelation with food trees and Orangutan nest. Researchwas conducted using line transect method, with variousrange of transect's length depending on the field conditions.Within each transect a 20m x 20m measurement plot,sample was established on each 100 m distance, called assub-plot. Within each of these sub-plots, the assessment oftrees with diameter 10 cm up were executed. Speciesdiversity index (Shannon-Winner), Evenness, Importantvalue index, Stands diameter classes were demonstrated,while analysis for Orangutan habitat was based on foodtrees and nest trees data and Orangutan density in swampforest.

Food trees, habitat quality, orangutan, tree species diversity

BO-12Linnaeus on swamp forest of Kapuas HuluDistrict,Yuliati Indrayani♥, Dwi Astiani, Wiwik EkyastutiFaculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email:mandaupermai@yahoo.com

Oil palm plantations in West Kalimantan grow in twodifferent habitats, laterite soil and peat land. On peat land,one of the pest insect have been attacking oil palmplantation is termite. This study aims to determine thediversity of termite species in oil palm plantations on westKalimantan peat land. Termite survey was conducted with100m × 2m transects according to Jones & Eggleton (2000)in each different water level (20, 30, 40, 50 and 60 cm).Termite attack levels were varying among five waterlevels. The higher water level showing the lower termiteattack level as shown in value 6%. While, the rest termiteattacks level were 18%, 14%, 14%, and 28% for waterlevel of 50 cm, 40 cm, 30 cm, and 20 cm respectively.Species richness of termite found in oil palm plantation onpeat land in West Kalimantan were Schedorhinotermesmedioobscurus, Schedorhinotermes sarawakensis,Havilanditermes Atripenis, and Coptotermes curvignathus,which belong to 3 genera (Schedorhinotermes,Schedorhinotermes and Coptotermes).

Oil palm plantation, peat land, species diversity, termites

BO-13Effect of rice seeds coated with biological agents toquality of seeds, and survival of Xoo and thebiological agents in the seedsTantri Palupi1,♥, Satriyas Ilyas2, MuhammadMachmud3, Eny Widajati2

¹Faculty of Agriculture, Universitas Tanjungpura, Jl. Achmad Yani,Pontianak 78124, Kalimantan, Indonesia. Telf/fax.: +62-561-740191,♥email: tantripalupi@yahoo.com.

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² Department of Agronomy and Horticultural, Faculty of Agriculture,Institut Pertanian Bogor. Jl. Meranti, Kampus IPB Darmaga, Bogor16680, Indonesia³Indonesian Research and Development Center for AgriculturalBiotechnology and Genetic Resources. Jl. Tentara Pelajar No. 3A, Bogor16111, Bogor, West Java, Indonesia

The research that consisted of two activities was performedat the Laboratory. The aim of experiment 1 is to obtainbiocontrol agents that have high resistence potential againstXanthomonas oryzae pv. oryzae (Xoo) and two biocontrolagents that are compatible to one another. While the aim ofexperiments 2 is to determine the effect of rice seeds coatedwith biocontrol agents to improve seed quality and reducelevels of Xoo infection. In the first experiment, fourisolates of bacterial deterrent, i.e: isolate Pseudomonasdiminuta A6; isolate P. aeruginosa A54; isolate Bacillussubtilis 11/C; and isolate B. subtilis 5/B, were tested fortheir resistence activities against Xoo on PSA plates usingthe method of growth inhibition zone with filter paper.Furthermore, the four deterrents were tested for theircompatibility with each other on PSA plates. In the secondexperiment, it used a completely randomized design with asingle factor (seed coating) consisting of seven levels,namely: negative control, healthy seed; positive control, theseeds contaminated with Xoo; P. diminuta A6 and B.subtilis 5/B; alginate 3% + 1% peat + P. diminuta A6 andB. subtilis % 5/B; arabic gum 3% + 1% gypsum + P.diminuta A6 and B. subtilis 5/B; CMC 1.5% + 1% talc + P.diminuta A6 and B. subtilis 5/B, and bactericidestreptomycin sulfat 20%. Results of Experiment 1 showedthat isolates P. diminuta A6 has the highest resistantpotential against Xoo based on diameter of inhibition zoneon Xoo, followed by isolate B. subtilis 5/B. Isolate P.diminuta A6 and B. subtilis 5/B had a good growthcompatibility with no resistence. Therefore, isolates P.diminuta A6 and B. subtilis 5/B were selected and used asbiocontrol agents in further studies. Results of Experiment2 showed that treatment with P. diminuta A6 and B. subtilis5/B gave the best result in increasing vigor of seeds, anddecreasing number of Xoo colony in the seeds.

Antagonistic, Bacillus subtilis 5/B, growth compatibility,inhibition zone, Pseudomonas diminuta A6, rice seeds coated

BO-14Preliminary evaluation of antitermitic activities ofwood vinegar from oil palm trunkH.A. Oramahi1, ♥, Tsuyoshi Yoshimura2, Farah Diba1,Nurhaida1

1Faculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email:oramahi_stp@yahoo.com

2 Research Institute for Sustainable Humanosphere (RISH), KyotoUniversity, Uji, 611-0011, Kyoto, Japan

Oil palm is one of the important agricultural wastes. Oilpalm plantations are spreading rapidly throughoutIndonesia and has been increasing the result in substantial

residue within oil palm industry. During replantation arefase with a big problem of disposal of fallen oil palm trunk.In vitro, antitermite activity of wood vinegar producedfrom oil palm trunk were evaluated. In this study,termiticidal properties of wood vinegar from oil palm trunkwas evaluated in laboratory for termite resistance tests.Three kinds of wood vinegar are produced at three differentpyrolysis temperature, i.e., at 350 (W1), 400 (W2) and450°C (W3). Five untreated and five treated wood blockswere exposed to subterranean termites (Coptotermesformosanus Shiraki) according to Japan Wood PreservingAssociation (JWPA) standard. All wood vinegar exhibitedantitermite activities to C. formosanus workers in the no-choice experiment. It needed relatively high concentrationto obtain the high mortality. For instance, the wood vinegarof 10 % concentration at temperature of 350 °C was neededto achieve 40, 20 % mortality against C. formosanus. Therewas significant difference between control and treatedwood vinegar of sugi wood (Cryptomeria japonica).

Antiterminte, oil palm trunk, resistance test, wood vinegar

BO-15Species diversity of macrofungi around RiamEngkuli in Pancur Aji tourism area, WestKalimantanHanna Artuti Ekamawanti♥, Ratna Herawatiningsih,Nila WidyasariFaculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email: hanna.artuti@gmail.com

Surveying species diversity of macrofungi was conductedaround Riam Engkuli in Pancur Aji tourism area, WestKalimantan. Twenty nine macrofungal species belonging to18 families and 23 genera were identified from 1.2 ha ofwhole sampling plots. Classifying fungal species byecological function showed 55% of collected species weresaprophytic, 28% ectomycorrhiza, 10% facultativesaprophytic and 7% parasitic. Based on their utilization,there were 13 edible species and 5 medicinal species. Atotal of 4, 397 macrofungal individu of basidiomycota andascomycota were recorded. Microporus xanthopusbelonging to family Polyporaceae was the most abundancespecies and used as ornamentation. Species diversity ofmacrofungi found in the observed area was low (diversityindex <1.5) with regularly distribution pattern (Moristaindex <1) and uneven abundance (evenness index <1). Thisstudy highlighted that macrofungal diversity is threatenedaround Riam Engkuli and needs for conservation measuresespecially for edible and medicinal species.

Ecological function, ectomycorrhiza, edible, medicinalmacrofungi, Microporus xanthopus

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BO-16Diversity of medicinal plant in Mandor,Pontianak, West Kalimantan for intructionalmedia in the shoolsReni Marlina♥, Ernita, SyamswisnaProgram of Biology Education, Faculty of Teacher Training andEducation, Universitas Tanjungpura. Jl. Prof. Dr. H. Hadari Nawawi,Pontianak 78124, West Kalimantan, Indonesia. Tel./Fax.: +62-561-740144, ♥email: reni.marlina@fkip.untan.ac.id

This research aimed to identify the diversity of medicineplants used by community in Mandor, Pontianak, WestKalimantan and to measure the validity of instructionalmedia of biodiversity concept in class X SMA. Thisresearch was a qualitative descriptive method. Theinstrument used in collecting data was the questionnaire,observation, and documentation. The sample in thisresearch was informants from three villages in the Mandordistrict. The sample consisted of 12 informants fromMandor village, 12 informants from Semenok village, and12 informants from Sekilap village. The ethnic sampleconsisted of 29 people Dayak, 4 people Chinese-Dayak,and Dayak-Malay, Malay and Java ethnic one personrespectively. The results showed that total plant speciesused as medicine by community in the Mandor, Pontianak,West Kalimantan was 83 species consisting of 48 families.Based on the validity criteria, the average total scores ofthe validation provided by the media experts were 3.6 andbelongs to valid category. Thus, it can be concluded thatthis media can be used as teaching materials in theclassroom.

Diversity, medicine plant, instructional media

BO-17Sweet corn yield and nutritive value of stover inthree growing season with manure inoculated bybiodecomposerDwi Retno Lukiwati♥, Endang Dwi Purbajanti, RetnoIswarin PujaningsihFaculty of Animal and Agricultural Sciences, Universitas Diponegoro.Kompl. drh. R. Soejono Koesoemowardojo, Tembalang Semarang 50275,Central Java, Indonesia. Tel./Fax.: +62-24-7474750, ♥email:drlukiwati_07@yahoo.com

Zea mays var. Saccharata (sweet corn) is an importantcrops grown for food and feed in the tropics. Manureenriched by rock phosphate (RP) and inoculated withbiodecomposer is very important to improve the soilfertility. The objective of the research was to investigatethe effects of some kind of manure on sweet corn yield andnutritive value of stover in three growing season. Acompletely randomized design with seven treatments andfour replicates was used in this experiment. The treatmentswere T1 (manure), T2 (manure+EM4), T3(manure+starTmic), T4 (manure+stardec), T5 (manuregranular+EM4), T6 (manure granular+starTmic), T7

(manure ranular+stardec). The manure was enriched withRP 66 kg P/ha and inoculated with the biodecomposeraccording to the assigned treatment. The manure wasapplied in rate of 30 t/ha. All plot received 125 kg K/ha and200 kg N/ha as basal fertilization. Result show that effectof some kind of manure was not significantly different tosweet corn yield. Manure inoculated with biodecomposerresults in significantly higher of nutritive value of stoverthan that without biodecomposer.

Biodecomposer, manure, nutritive value, stover, sweet corn

BO-18Impacts of timber harvest on Hymenopterabiodiversity in a natural production forest in EastKalimantan, IndonesiaAhmad Budiaman1,♥, Noor Farikhah Haneda2, ImaDuwi Robaikah1

1Departement of Forest Management, Faculty of Forestry, InstitutPertanian Bogor. Kampus IPB Darmaga, Bogor 16680, West Java,Indonesia. Tel./Fax.: +62-251-8626806/+62-251-8626886. ♥email:2Department of Silviculture, Faculty of Forestry, Institut Pertanian Bogor.Kampus IPB Darmaga, Bogor 16680, West Java, Indonesia

Hymenoptera is one of the major components of insectbiodiversity. Timber harvest, as part of forest management,leads to change the Hymenoptera biodiversity. This studyanalyzes the impacts of timber harvest on the Hymenopteradiversity in a production forest in East Kalimantan ,Indonesia . In this study, the diversity of Hymenoptera wascompared before and after felling in three different fellingintensity (low, medium and high) . The results showed thatthe timber harvest tend to decrease the abundance ofHymenoptera. Diversity and species richness ofHymenoptera will decline in moderate and high fellingintensity, whereas evenness index of Hymenopteraunchanged at various felling intensity. The composition ofmorphospecies of Hymenoptera was changed after fellingwith high intensity

Biodiversity, Hymenoptera, natural production forest, timberharvest

BO-19New record of wild banana (Musa balbisiana) fromWest KalimantanAri Sunandar♥

Program of Biology Education, Faculty of Teacher Training andEducation, Universitas Tanjungpura. Jl. Prof. Dr. H. Hadari Nawawi,Pontianak 78124, West Kalimantan, Indonesia. Tel./Fax.: +62-561-740144, ♥email: arisunandar031@gmail.com

Kalimantan Island is a center of origin and diversity of wildbananas in Indo-Malesia region. West Kalimantan is one ofprovince in Kalimantan Island. However, only a littleinformation is available about diversity of wild bananas in

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West Kalimantan. The aim of this study was to describe thediversity of wild bananas species in Nipah Panjang andTeluk Nibong villages, Kubu Raya district. By examiningthe morphological character of digital specimen, onespecies of Musa was identified. Musa balbisiana was a newrecord of wild banana species from west Kalimantan.Previously, Musa balbisiana is widely distributed in Javaand Sulawesi only. Musa balbisiana were found inroadside or backyard of resident in Teluk Nibong village.

Musa balbisiana, West Kalimantan, wild bananas

BO-20Quick assessment method for calculating softshellturtle (Amyda cartilaginea) and reticulated python(Python reticulatus Schneider, 1801)Mariana Takandjandji♥, Hendra Gunawan, Vivin S.SihombingForest Research and Development Center, Ministry of Environmental andForestry. Jl. Gunung Batu No. 5. PO Box 165, Bogor 16001, West Java,Indonesia. Tel. +62-251-8633234; 7520067. Fax. +62-251 8638111.♥email: rambu_merry@yahoo.co.id

Softshell turtle (Amyda cartilaginea Boddaert, 1770) andreticulated python (Python reticulatus Schneider, 1801) arethreatened in wildlife because they are traded nationallyand internationally. The government has set a quota for theharvesting and trading of these reptiles but these has notbeen accompanied by a preservation which can lead topopulation declines and extinctions, while the number ofpopulation in the wild is not known with certainty. Thepurpose of this research is to determine the method of rapidassessment for the basis to set a quota utilization ofreptiles, especially for softshell turtle and reticulatedpython. The study was conducted in East Kalimantanthrough deep interview survey with 50 respondents(exporters, fishers, traders, collectors) and 15 keyrespondent (government). The results showed that they hadharvested from the wild because there has been nosuccessful breeding. The harvest was dominated by femaleadult softshell turtle with a number of harvesting in KotaBangun area reaching 1, 080/year (1/3 of an export quotafor East Kalimantan). Softshell turtle from Kota Bangunhas a bigger posture than the turtle from West Java andJakarta and reticulated python from Kota Bangun waslonger than the reptiles from West Java and Jakarta.

Softshell turtle, reticulated python, quotas, quick assessment

BO-21The spatial change of the tropical lowland forestand its possible effect on biodiversity changes inSouth Sumatra, IndonesiaZulfikhar1, ♥, Zulkifli H.2, Kadir S.3, Iskandar I.4

1Graduate School of Environmental Sciences, Universitas Sriwijaya. Jl.Palembang Prabumulih Km 32, Indralaya Ogan Ilir, South Sumatra,Indonesia. ♥email: zulfikhar.zl@gmail.com2Department of Biology, Faculty of Mathematics and Natural Sciences,Universitas Sriwijaya. Jl. Palembang Prabumulih Km 32, Indralaya OganIlir, South Sumatra, Indonesia ywindusari@yahoo.com3Department of Soil Science, Faculty of Agriculture, Jl. PalembangPrabumulih Km 32, Indralaya Ogan Ilir, South Sumatra, Indonesia4 Department of Physics, Faculty of Mathematics and Natural Sciences,Universitas Sriwijaya. Jl. Palembang Prabumulih Km. 32, Indralaya OganIlir, South Sumatra, Indonesia

The forest fragmentation is a process of spatial changesresulting in reduction of the number and size of forestpatches, and subdivision and breaking up of patches intosmaller parcels. This process leads to edge effect, forestisolation, reduction of the species population, andbiodiversity loss. The objective of this study is to examinethe possible effect of changes in spatial pattern of habitatcontinuum on the changes of the tree species diversityindice. We applied the spatial change analysis of forestfragmentation, that determine the principle component ofthe factors of the forest landscape metrics indicatorchanges. We take an advantage of multitemporal satteliteimagery from 1989 to 2013, it has been clip as a unit areaof analysis into 32 unit, circular shape with a radius of 2,500 m, and observations from 32 sample plots, size 20 x 50m, at the midpoint of the clip. The results show that thereare seven main indicators of forest fragmentation observedin the study area, having major role on the change indiversity indice of tree species, those are largest patchindex, effective mesh size, total edge, clumpiness index,patch richness, patch contiguity index and patch cohesionindex of the landscape structure, that have a major roles tochange in the forest trees species richness, evenness anddiversity indices. Multiple regression equations with thedependent variable of Shannon's and Wienner Index and asthe independent variable of main indicators of landscapemetrics indicators, shows the R2 = 0.988 and significantlevel F change less than 0.05. We may expect the result ofthe research findings, to determine how to manipulate thenatural forest landscapes to improve the potential for rangeexpansion on the forest management plan, controlling ofdeforestation, and in the forest restoration activities.

Fragmentation, landscape metrics, diversity indices

BO-22Mononchid and dorylaimid nematodes fromBanua Botanic Garden, South KalimantanAuliya Hanifa Permata♥, Abdul GafurDepartment of Biology, Faculty of Mathematics and Natural Sciences,Universitas Lambung Mangkurat. Jl. A. Yani Km. 36, Banjarbaru 70714,South Kalimantan, Indonesia. email: permatapertama674@yahoo.com

In view of the limited number of studies on soil nematodesfrom South Kalimantan, the present study was aimed todescribe nematodes from Banua Botanic Garden in SouthKalimantan. Soil samples were taken at a depth of up to 20cm, and nematodes were extracted using Whitehead and

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Hemming filtration method. Permanent slides of nematodeswere prepared in applying Seinhorst’s quick method. Atotal of three species were found belonging to the generaAporcelaimellus, Xiphinema (Dorylaimida) and Iotonchus(Mononchida). The presence of dorylaimid genera arerecorded for the first time from South Kalimantan.

Aporcelaimellus, Banua Botanic Garden, Dorylaimida,Monochida, Xiphinema, Identification, Iotonchus

BO-23First records of species of the genera CobbonchusAndrassy, 1958 and Mylonchulus Cobb, 1916(Nematoda: Mononchida) from South KalimantanAbdul Gafur♥

Department of Biology, Faculty of Mathematics and Natural Sciences,Universitas Lambung Mangkurat. Jl. A. Yani Km. 36, Banjarbaru 70714,South Kalimantan, Indonesia. email: agafur@unlam.ac.id

Previous survey of nematodes in Kabupaten Banjar SouthKalimantan left a number of specimens unidentified. In thepresent study, two species of mononchids belonging to thegenera Cobbonchus Andrassy, 1958 and MylonchulusCobb, 1916 are described, viz. Mylonchulus brachyuris(Bütschli, 1873) Cobb, 1917 and Cobbonchus collarisAndrassy, 1985. These are first records of occurence of thetwo species in South Kalimantan. Morphological charactersof the two species generally fit the original and furtherdescriptions, with some deviations which are hereconsidered intraspecific variations, leading toredescriptions of the corresponding species.

Description, identification, Mononchida, predatory nematodes

BO-24Length–length and length–weight relationships ofIndian mackerel Rastrelliger kanagurta (Cuvier,1817) from the Mayalibit Bay, Raja Ampat, WestPapua, IndonesiaDian Oktaviani♥, Setiya Triharyuni, Duto NugrohoCenter for Fisheries Research and Development, Agency for Marine andFisheries Research and Development, Ministry of Marine Affairs andFisheries, Gedung Balitbang KP II. Jl. Pasir Putih 2 Ancol Timur, JakartaUtara 14430, Jakarta Indonesia. Tel.: +62-21-64700928 Fax.: +62-21-64700929, email: dianoktavianni@gmail.com

We present the relationships between total (TL), fork (FL),standard (SL) length and the relationships between TL, FL,SL and wet weight for Rastrelliger kanagurta from theMayalibit Bay (Raja Ampat; Indonesia). The relationshipsbetween TL, FL and SL were all linear (for all cases: r2>0.994). The values of the exponent b of the three lengths–weight relationships range from 3.421 to 3.494, 2.744 to3.008 and 3.502 to 3.633 for all size, mature size andimmature size, respectively. The equation of mature sizedata (F=2.900; P=0.092; r= 0.998; n=39) is the most

representative than equation of all size data(F=1.269;P=0.263; r= 0.995; n=39) and equation of immature sizedata (F=0.782; P=0.379; r= 0.993; n=39) to describe onlength weight relationship of size (from juvenile to maturesize) of R. kanagurta.

Length, Rastrelliger kanagurta, weight, West Papua

BO-25The prospects of utilization for bungur wood bychemical component analysisHikma Yanti♥, Evy Wardenaar1Faculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email:yanti_hikma@yahoo.com

Bungur (Lagerstroemia speciosa) is a kind of shrubs whichcan be found in various tropical areas and can growanywhere, even on arid land. Typically, bungur is plantedas an ornamental or shading trees along the highway. Thisstudy aims to determine the prospects of utilization ofbungur wood by chemical components in various positions,height and depth rod including extractive of soluble alcoholbenzene, holocellulose, alpha cellulose and lignin. Basedon the location of the height rod, the results of the analysisobtained a mean value of extractive substances solublealcohol benzene ranged from 2.4526 to 4.6609%;holocelullose ranged from 55.2049 to 72.5229%; alphacellulose ranged from 41.4495 to 61.4212% and lignincontent ranged from 36.7999 to 37.8595%. While based onthe location of the depth of the bar, it showed the value ofextractives of soluble alcohol benzene ranged from 2.5127to 4.6757%; holocelullose ranged from 57.9500 to65.3283%; alpha cellulose ranged from 46.9021 to52.9249% and lignin content ranged from 36.4205 to38.0241%. And based on the analysis of chemicalcomponents, holocelullose and alpha cellulose content washigh, so it makes bungur wood excellent to be used as rawmaterial for pulp and paper.

Alpha cellulose, bungur, extractive, holocelullose, lignin

BO-26Habitat characteristics and distribution pattern ofunderstory with anticancer potential in CibodasResort Area, Mount Gede Pangrango NationalPark, IndonesiaYanieta Arbiastutie1,2, ♥, Ris Hadi Purwanto3, Mae SriHartati2, Joko Marsono2

1Doctoral Program of Forestry Science, Faculty of Forestry, UniversitasGadjah Mada. Jl. Agro No. 1 Bulaksumur, Sleman 55281, Yogyakarta,Indonesia. ♥email: yanie_ta@yahoo.co.id2Faculty of Forestry, Universitas Gadjah Mada. Jl. Agro No. 1Bulaksumur, Sleman 55281, Yogyakarta, Indonesia

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3Faculty of Medical, Universitas Gadjah Mada. Jl. Farmako Sekip Utara,Sleman 55281, Yogyakarta, Indonesia

Understory development as the anticancer agent is an effortto optimize the potential of forest resource that can befound in National Park area. Besides the information aboutits metabolite content, this effort has to be supported by theinformation about its habitat characteristics and distributionpattern. It is important to realize the conservationmanagement of biological resource. This study aims toidentify the habitat characteristics and distribution patternof understory with anticancer potential in Mount GedePangrango National Park. The location of study is inCibodas Resort area which is one of the utilization zones.Identification of the understory with anticancer potential isconducted by exploring for taking the sample of understoryleaves. Then, they would be tested in the laboratory toidentify the understory species that has real anticancerpotential based on the result of cytotoxic test andphytochemical test. The result of laboratory test would bethe basic information to conduct the field observation instudying the habitat charactersitics and distribution patternof the understory with real anticancer potential by usingstratified sampling. The result shows that there are 5understory species with real anticancer potential form 97species collected. They are Physalis peruviana L., Tithoniadiversifolia (Hemsl.) A. Gray, Lantana camara L.,Clidemia hirta (L). D.Don., and Solanum torvum Sw.According to the result of field observation, these spesiescould be found in sub montana ecosystem with the range ofelevation between 1,300 m asl to 1,500 m asl. Theoptimum temperature supporting these species reaches24.20C to 25.36 0C with the air humidity between 72.94 %to 74.66 %. These species grew well in andosol soil withpH level reaching 5.74 to 6.42. The result of study alsopresented there are the differences in ditribution pattern ofthese species. Tithonia diversifolia (Hemsl.) A. Gray,Lantana camara L., and Solanum torvum Swhad convergendistributions that are stated by Morisita Index higher than1, but Physalis peruviana L. and Clidemia hirta (L).D.Don.have regular distribution that are valued by Morisita Indexlower than 1

Anticancer, distribution pattern, habitat, national park, understory

BO-27Etlingera elatior (Jack) R. M. Smith: Uses,phytochemicals, and biological activitiesMarina Silalahi♥

Department of Biology Education, Faculty of Education and TeacherTraining, Universitas Kristen Indonesia. Jl. Mayjen Sutoyo, RT.9/RW.6,Cawang, Kramatjati, Jakarta Timur 13630, Jakarta, Indonesia. Tel.: +62-21-1800919, ♥email: marina_biouki@yahoo.com

Etlingera elatior (Jack) R. M. Smith is a plant in Indonesiawhich is used by Indonesian ethnic as medicine, spices,vegetable, and ornamental. Utilization of E. elatior as amedicine and a spice is more prominent than other use.This article aims to explain the uses, phytochemicals, and

biological activity of E. elatior. This paper is based onoffline literature and online media. Offline literatures basedon handbooks, dissertations and theses. Web, Scopus,Pubmed, Journal, and other online media is as supplementin this article. Etlingera elatior is also used by localcommunities in Indonesia as vegetables, or condiments, orumbut mixture, or seasoning arsik. The food which isadded by E. elatior has a distinctive aroma and is moredurable. As medicine, E. Elatior is used to cure fever,cough, ear infections, wound, antioxidant, antitumor,cytotoksin, anti-hypertension, diabetes mellitus, anti-aging,anticancer, and traditional sauna materials. Utilization of E.elatior as a medicine and spices is related to secondarymetabolites content. Essential oils found in E. elatior areesspecially mono and sesquiterpenoids, which haveproperties to produce volatile odours

Biological activities, Etlingera elatior, phytochemicals

BP-01Size distribution and biology aspect of thescalloped hammerhead shark (Shyrna lewini) IinTanjung Luar, East Lombok, IndonesiaRia Faizah♥

Center for Fisheries Research and Development, Agency for Marine andFisheries Research and Development, Ministry of Marine Affairs andFisheries, Gedung Balitbang KP II. Jl. Pasir Putih 2 Ancol Timur, JakartaUtara 14430, Jakarta Indonesia. Tel.: +62-21-64700928 Fax.: +62-21-64700929, email: faizah.ria@gmail.com

The scalloped hammerhead shark (Shyrna lewini) is one ofmember of Shyrnidae family that is highly mobile,susceptible to overharvesting and have important economicvalue. This research aims to obtain data and informationabout the size distribution and biological aspects of thescalloped hammerhead sharks as a first step to determinemanagement measures. This research was conducted inMarch, August, December 2010, January and October 2011in the PPI Tanjung Luar, Lombok Timur. Data werecollected from the surveyed areas including the frequencyand the sex composition. The results showed that thefemale of the scalloped hammerhead sharks were caughtmore frequently than males during the observation periodwith the sex ratio of 2.41: 1. The size distribution of thescalloped hammerhead sharks females and males werebetween 51-310 cmTL with average length of 208.68cmTL. The highest number of hammerhead sharks wasfound in December 2010 and January 2011 which wasbetween 30 to 40% of total catches during of study. Thereis a positive relationship between the total length and theclasper length.

Clasper length, size distribution, sex ratio, scalloped-hammerheadshark

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BP-03Inventory fish catch fishermen in the Village ofPadang Tikar, West KalimantanEko Sri Wahyuni♥, Linda RianaFaculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email: ekosri_w83@yahoo.co.id

This study aims to determine the types of fish caught byfishermen in the villages of Padang Tikar West KalimantanProvince. Forms of this study is qualitative and the methodused is descriptive with purposive sampling technique. Thetechnique of collecting data is interviews, observation, anddocumentation. Based on the results, 32 species of fishbelonging to the 24 families are found.

Inventory, fish, Padang tikar, West Kalimantan

BP-04Population study of freshwater crayfish, Cheraxspp., from Indonesian Papua based onmorphometrict charactersAbinawanto1,♥, H. Hamidah1, A. Laksono1, R.Eprilurahman2,♥♥

1Department of Biology, Faculty of Mathematics and Natural Sciences,Universitas Indonesia. Kampus UI Gedung E Lt. 2, Jl. Lingkar KampusRaya, Depok 16424, West Java, Indonesia. Tel.: +62-21-7270163 Fax. :+62-21-78849010. ♥email:abinawanto.ms@sci.ui.ac.id2Faculty of Biology, Gadjah Mada University. Jl. Teknika Selatan, SekipUtara, Sleman 55281, Yogyakarta, Indonesia. ♥email:rurybiougm@ugm.ac.id

The aim of the study was to determine morphometriccharacters of fresh water crayfish, Cherax spp. Sampleswere collected from Uter lake (Atinjo district), Seremukriver (Haha village), Baliem river, (Hubukiak district,Jayawijaya), and Baliem river (Wesaput village, Wesaputdistrict), respectively. The morphometric measurementconsisted of two parameters, truss cell and truss length,respectively. Truss cell were divided into four parts, A, B,C, and D. Each part consisted of six lanes, which wascalled truss length, and was numbered from one to six.Length measurement was carried out using digital caliper.Either univariate or multivariate data were analyzed byprincipal component analyses (PCA) and canonicalanalyses. The result showed that the collected samplesoriginated from two population groups, Papua and WestPapua, respectively.

Fresh water crayfish, morphometric characters, population study

BP-05Morphometric characterization of mantis shrimps,Harpiosquilla harpax and Oratosquilla oratoriacollected from Pelabuhan Ratu waters, IndonesiaAbinawanto♥, D. Iftitah, W. Wardhana

Department of Biology, Faculty of Mathematics and Natural Sciences,Universitas Indonesia. Kampus UI Gedung E Lt. 2, Jl. Lingkar KampusRaya, Depok 16424, West Java, Indonesia. Tel.: +62-21-7270163 Fax. :+62-21-78849010. ♥email:abinawanto.ms@sci.ui.ac.id

The aim of the study was to determine two species,Harpiosquilla harpax and Oratosquilla oratoria whichwere collected from Pelabuhan Ratu waters. Based on 11morphometric characters, the total length (TL) of 14 malesof H. harpax was 141.59 mm, whereas TL of 20 femaleswas 171.07 mm, respectively. While the TL of 4 females ofOratosquilla oratoria was 98.78 mm. The other parametersmeasured were carapace length, total weight, abdominallength, telson length and claw length. Data were analyzedby multivariate analyses using SPSS version 17. Thefemale size was bigger than males, it had correlation to theenergy requirement for female maturation.

Harpiosquilla harpax, morphometric characters, Oratosquillaoratoria, Pelabuhan Ratu waters

Diversity of ecosystem

CO-01Forest type diversity on carbon stocks: Cases ofrecent land cover conditions of tropical lowland,swamp, and peatland forests in West KalimantanDwi Astiani1, ♥, Mujiman2, Rafiastanto3

1Faculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email: astiani.dwi@gmail.com2Lembaga Landscape Livelihood Indonesia (LLI), Pontianak, WestKalimantan, Indonesia3Fauna and Flora International-Indonesia Programme, Jakarta, Indonesia

Tropical forests constitute for a large concentrated carbonpools, ultimately in tropical peatland forest since this foresttype sink carbon both in the vegetation and its underlyingpeat. However, these forests recently experienced a lot ofpressures from anthropogenic disturbances. A study wasconducted to estimate carbon stocks of degraded tropicallowland, swamp, and peatland forests in Kayong UtaraWest Kalimantan. The study area was classified based onthe canopy closures and forest/landcover types and groupedinto low and high degraded lowland forest, low and highdegraded peat forest, low and high degraded swamp forest,shrub land, and mixed agricultural land. Aboveground and

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belowground biomass and carbon stocks in each group wasestimated. Results showed that among the groups, thehighest to the lowest order of aboveground carbon sinkwere ranked from 255.9 down to 36.9 ton C/ha, plus otherbiomass sources recruited from necromass, litter, andground storey vegetation. It is demonstrated that forestdegradation and land cover changes had reduced theamount of above ground carbon stocks and thus couldresult large amount of carbon loss from forests. It isestimated that 22, 600 ha area of overall forest types/landcovers sink ~2.5 million of aboveground and ~5, 570 hapeatland area hold ~9.2 million of below ground carbon.This amount of carbon is a potential sink of carbon, but itcould become a huge losses if land cover changescontinued.

Aboveground carbon, belowground carbon stocks, degradedforest, forest types land cover change

CO-02Sustainable utilization of forest resources in theWest Kalimantan and Sarawak border region;Strategy for optimal local workforce absorptionNurul Bariyah♥

Faculty of Economics and Business (FEB),Universitas Tanjungpura. Jl. Ahmad YaniPontianak 78124, West Kalimantan, Indonesia. ♥email:nurulb_hamzar@yahoo.com

This is a descriptive study to elaborate local employmentconditions among border communities in West Kalimantanand prepare labor optimization model based onobservations and interviews with sources and relevantagencies in relation to the scenario of sustainable use ofnatural resources to improve the welfare of bordercommunities by promoting cultivation of gaharu and wildbee honey, especially in conservation area. The borderregion between West Kalimantan and Sarawak has richforest stand that are relatively saved from plunder by steepslope of the Muller-Schwaner mountain range. Howeversince forest stand can be reached easily, they become lessand less available. Forests in remote area and difficult toaccess areas are no longer immune from extraction anddestruction. Forests have been used and abused for itswood for several decades, and forest’s other potentialvalues and usefulness have also gone when the trees are cutdown. The situation leads to worker migration across theborder to Malaysia to find jobs. Respondents reported thatthe condition of local forest are relatively guarded and thelocal population are still using the forest for collectingvarious products such as rattan, barks of Tarap trees,bamboo, and barks of Kapuak tree (spun to maketraditional cloth). The respondents said that gaharu tree isalmost extinct from their forest, however they still keepfresh memory when gaharu expedition used to be a bigevent in their economic lives. Collecting wild bee honeyused to be an important part of local economy, but withdiminishing existence of tree stands, local bees are

migrating and it causes significant decrease in local honeyproduction. People are eager to try some methods practicedin other areas that have been successful in boostingproduction of wild bee honey. The efforts might solveunemployment problem as well as illegal worker migrationto Malaysia.

Border, employment, gaharu, wild bee honey

CO-03Role of soil microbes in increasing plant growth ofJatropha curcas in soil contamibated mercuryWiwik Ekyastuti♥, Hanna Artuti EkamawantiFaculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email:wieky_serdam@yahoo.co.id

Every plant has a different respone to the mercury in theenvironment. From the previous research, we found thatjatropha (Jatropha curcas) is a plant that is tolerant tomercury, so it could potentially be used as aphytoremediator of mercury. On the other hand, some typesof rhizosphere microbes such as arbuscular mycorrhizalfungi (AMF) and mercury reducing bacteria (MRB) alsohave the ability to reduce heavy metals including mercury.Symbiosis between the microbes with jatropha is not yetknown in improving plant growth. The purpose of thisresearch was to determine the role of microbes namelyAMF and BRM in enhancing the growth of jatropha inmercury contaminated soil. The research was conducted intwo places, namely in the greenhouse and in the field (thetailings of ex-illegal gold mining). The research wasconducted using factorial experiment with a completelyrandomized design. In both locations of planting, therewere two types of microbes. They were AMF with 4 levelsof factors and MRB with 3 levels of factors. Eachcombination treatment was repeated four times in thegreenhouse and ten times in the field. Mercuryconcentrations in the media were 10 ppm. The resultsshowed that in general, the interactions between AMF andMRB are simultaneously able to increase the growth ofjatropha not only in the greenhouse but also in the field. Itis also known that both types of AMF Glomus SS18 andMRB Bacillus sp is highly recommended to be inoculatedsimultaneously to tailings (soil) contaminated by mercury.

Illegal gold mining, Jatropa curcas, soil microbes, tailing

CO-04Carbon sequestration of bakau (Rhizophoraapiculata) tree planted in Kubu Raya District,West KalimantanRinto Wiarta♥, Dwi Astiani, Yuliati Indrayani, FairusMulia

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1Faculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email: rinto_wiarta@yahoo.com2 PT Bina Ovivipari Semesta, Kubu Raya District, West Kalimantan,Indonesia

Mangrove forest ecosystem has known for its role inbuffering coastal abration and reservoiring seafoodproduction. However, information on its role insequestering carbon in trees is very little. This study aimsto determine carbon sequestration on one to five years oldRhizophora apiculata Blume tree species planted in PT.Bina Ovivipari Semesta (PT. BiOS). This study wasconducted over 5 months in 2015 in the area ofrehabilitation that purposively selected. This study uses amethod of harvesting and no harvesting with purposivesampling system. The results showed that mangrovespecies has an ability to absorb very high carbon. Theamount of carbon sequestered by these 1 year old plants, 3years old and 5 years old were 0.363 tons/ha, 5.591 tons/haand 7.240 tons/ha, consecutively.

Carbon sequestration, mangrove ecosystem

CO-05Differentiation of soil organisms at different typesof peatland in West KalimantanRossie Wiedya Nusantara♥, Asripin AspanDepartment of Soil Science, Faculty of Agriculture, UniversitasTanjungpura. Jl. Prof. Hadari Nawawi, Pontianak 78121, WestKalimantan, Indonesia. Tel.: +62-561-785342, 583865, Fax.: +62-561-765342, email: rwiedyanusantara@gmail.com

Peat land conversion could threaten the existence of soilorganisms which is influenced by soil physical properties.The objective of this study was to analyze the changes insoil organisms of land-use due to the conversion ofpeatlands in West Kalimantan. The study was conducted insecondary peat forest (SPF), shrub (SB), oil palmplantation (OPP) and corn-field (CF) in Kubu Raya Districtof West Kalimantan. Stages in this study includeobservation of water-table depth, soil temperature andanalysis of physical properties include bulk density,moisture and porosity.The results indicated that a greaterdecline in colonies of bacteria, fungi and earthworms inOPP than in SPF, respectively 50%, 53% and 68.8%. Thedecline in the condition of affected water content is due toland conversion of secondary forests into oil palmplantations. In addition, it was also characterized byconditions on the water-table depth which was deeper andsoil temperature which was higher in OPP than SPF. Thiscondition was the opposite in CF, where both bacteria andfungi is increased 53% and 33.3% respectively whileearthworms are decreased (6.3%). The different conditionis that it was characterized by water-table depth which wasshallow and the water content which was higher eventhough the temperature was almost the same between OPPand CF.

Land-use change, soil temperature, soil organisms, water-tabledepth

CO-06Ecological study of Nepenthes pitopangii; A rareand endemic flowering plant of Central Sulawesi,IndonesiaRamadhanil Pitopang1,♥, Irmasari Thaha2

1Department of Biology, Faculty of Mathemathics and Natural Sciences,Universitas Tadulako. Kampus Bumi Tadulako Tondo, Jl. Sukarno HattaKm 8, Tondo Palu, Indonesia. ♥email : pitopang_64@yahoo.com2 Department of Forest Management, Faculty of Forestry, UniversitasTadulako. Kampus Bumi Tadulako Tondo, Jl. Sukarno Hatta Km 8,Tondo Palu, Indonesia

Central Sulawesi is one of the provinces in Indonesia,located in Sulawesi Island (formerly known as Celebes),the main island in “Wallacea biogeography region, an areathat has the potential of a unique biological resources richin endemic flora and fauna. Most studies on plantbiodiversity in Sulawesi have focused on Taxonomic andEcological aspects (Pitopang et al., 2002, 2004, 2007,2008, 2011, 2012; Ramadhanil et al., 2008 ; Pitopang, 2009; Cannon et al., 2007; Kessler et al., 2012a, 2012b ; Kessleret al., 2005; Gradstein et al., 2005; Mogea, 2004, 2005;Thomas, 2010; Thomas et al., 2011 ; Ciccuza et al., 2010,2011 ; Culmsee and Pitopang 2009; Culmsee et al., 2010,2011; Poulsen, 2014), but the research on the subject ofethnobotany is very few (Purwanto, 2004; Pitopang andSarifuddin, 2012; Paik et al., 2013; Gailea et al., 2016 ;Fathurahman et al., 2016). Nepenthes pitopangii Lee(Nepenthaceae) is one of endemic flowering species fromCentral Sulawesi, just published as a new species forscience that is collected by Ramadhanil Pitopang, theCurator of the Herbarium Celebense (CEB) in the montaneforest of Lore Lindu National Park, Central Sulawesi. Onthe one hand, the population of this plants in their naturalhabitat is very small and also the information on somebotanical aspects like taxonomy, ecology, anatomy andmolecular data of Nepenthes pitopangii are not yetavailable. Based on preliminary observation, it is showedthat there are some species of trees that grow in itssurrounding habitat such as : Helicia celebica (Proteaceae),Neolitsea celebica (Lauraceae), Phylocladus hypophyllus,Adinandra celebica, Drymis piperita, Quintinia apuensis,Castanopsis accuminatisima, Eurya accuminatisima,Acromychia trifoliata (Rutaceae), Gastonia serratifolia(Araliaceae). Pinanga caesea (Arecaceae), Santiria sp,Acmena accuminatisima (Myrtaceae), Listea feruginea(Lauraceae), Pittosporum moluccanum. and some fernsspecies such as ; Gleichenia truncata, Lycopodiella cernua,and Dipteris conyugata. Environmental parameters such asaverage daily temperature, humidity and rainfall whichwere 17, 1o C, 90.2% and 351, 1 mm. The nitrogen andposphor content in soil are very low.

Central Sulawesi, endemic, Nepenthes pitopangii

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CO-07Adoption of agroforestry patterns on communityforest in Nusapati Village, West Kalimantan,IndonesiaEmi Roslinda♥, Wiwik Ekyastuti, Siti MasitohKartikawati, Syarifah1Faculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email: eroslinda71@gmail.com

To maximize the ecological and economic function ofcommunity forest program, the role of communities mustbe involved, especially in managing the forest byoptimizing the community forest and applying agroforestrybased on socio-economic conditions in the community.Based on these condition, it is necessary to hold a researchconcerning to level of community preference to the type ofplants and the level of adoption, as well as the NPVanalysis of the patterns of agroforestry which are suggestedand apllied by community. Research on adoption ofagroforestry patterns on community forest on Nusapativillage was conducted, using survey methods. The sampleconsisted of 11 village officials, 23 farmers, 8 women, 4religious leaders and 14 youth leaders (karang taruna).Result of the study revealed that there were 4 dominantplants types prefered by the community, namely:eaglewood, pineapple, banana, and rubber. Based on theseplants, there were 2 agroforestry patterns practiced by themajority of community in their community forest, theywere: (1) eaglewood-banana-fruit, and (2) rubber-pineapple-fruit. Beside that, at this time, it is advisable toseek the agroforestry system with a mixture of eaglewood,banana and honey kelulut. The highest personal and socialNPVs are needed in applying the agroforestry pattern ofeaglewood-banana-honey kelulut. To ensure thesustainability of the program, the development ofcommunity forest together with a program of communityempowerment are recommended to continue based onecological and economic condition.

Agroforestry, community forest, ecology, economy, honey kelulut

CO-08Sago palm as a potential crop for peat restorationin West KalimantanEvi Gusmayanti1, ♥, Gusti Z. Anshari2, M. Pramulya3,Sholahuddin4, Novira Kusrini5

1Department of Agronomy, Faculty of Agriculture, UniversitasTanjungpura. Jl. Prof. Hadari Nawawi, Pontianak 78121, WestKalimantan, Indonesia. Tel.: +62-561-785342, 583865, Fax.: +62-561-765342, email: vie.untan@gmail.com2Department of Soil Science, Faculty of Agriculture, UniversitasTanjungpura. Jl. Prof. Hadari Nawawi, Pontianak 78121, WestKalimantan, Indonesia3Department of Food Science and Technology, Faculty of Agriculture. Jl.Prof. Hadari Nawawi, Pontianak 78121, West Kalimantan, Indonesia4Department of Agribussines, Faculty of Agriculture, UniversitasTanjungpura. Jl. Prof. Hadari Nawawi, Pontianak 78121, West

Kalimantan, Indonesia5Master of Environmental Studies, Graduate Program, UniversitasTanjungpura. Jl. Prof. Hadari Nawawi, Pontianak 78121, WestKalimantan, Indonesia

The peat restoration is becoming popular after theestablishment of Peat Restoration Agency which is aimedto restore the function of peatland ecosystem. Therestoration is not about re-flooding the drained peatland,but more to manage the peatland so the economic, socialand environmental aspects will work in harmony. One ofapproach may be applied in peat restoration is re-plantingthe open or degraded peatland with adaptable crop such assago palm. According to the literature, Indonesia hasapproximately one million hectare of sago palm areas,which is about a half of total sago areas in the world. Someof benefits of sago palm are high starch productivity(potentially about 30 ton starch/ha/year), huge canopy(indicates huge carbon sequestration potential), lowmaintenance (low fertilizer and low water tablerequirement imply low greenhouse gas emission potential)and old traditional crop (indicate the social acceptance).Despite of these potential advantages, real condition ofsago palm may found different. According to field study atsmall holder of sago palm plantation in District of KubuRaya, West Kalimantan, sago palm still has opportunity toincrease its productivity in term of starch productivity(which around 10 ton starch/ha/year) and carbonsequestration rate which is indicated by low biomassdensity (about 700 ton carbon/ha). Improvement oncultivation practices and management may improve sagopalm productivity performances as required for peatlandrestoration

Peat restoration, sago palm, West Kalimantan

CO-09Effect of space planting and organic fertilizer ontalas beneng plant (Xanthasoma undipes)Kartina AM♥, Apriany Fatmawaty, Nuniek Hermita,Eltis Panca NingsihDepartment of Agroecoteknology, Faculty of Agriculture, UniversitasSultan Ageng Tirtayasa. Jl. Raya Jakarta Km. 4, Serang, Banten,Indonesia. ♥email: kartina_plg@yahoo.com

Talas beneng (Xanthasoma undipes K.Kock) or also knownas Tall elephant ear is a typical local taro from MountKarang, Banten province, which belong to the genusXanthosoma. It has been developed as a local food source.Good quality of taro seedlings were obtained throughintensive maintenance especially row planting andfertilization. Fertilizers are needed for the growth of talasbeneng seedlings. It includes organic fertilizer. Theobjective of this experiment is to determine the effect ofplant spacing and organic fertilizers for talas benengseedling. This experiment was conducted at around MountKarang, Banten province, from April 2016 to Agustus2016. The experimental design used was factorialrandomized block design with three replications. The first

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factor was row planting i.e., 0.5 m x 1 m, 1 m x 1 m and 1,5 m x 1 m. The second factor was the plants which wasgiven by organic fertilizer, i.e., 20 tons/ha of chickenmanure fertilizer, 20 tons/ha of cow manure fertilizer and20 tons/ha of compost. The results showed that rowplanting affected plant height at 8 Week AfterTransplanting (WAT) and steam diameter (6-12 WAT),whereas organic fertilizer affected plant height, leafnumber and steam diameter of talas beneng seedling.compared to other treatments, talas beneng seedlings growbest at a spacing of 1 m x 1 m. And, they grow best atcompost giving of 20 tons/ha.

Fertilizer, organic, seedling, taro

CO-10A study on the potential for ecosystems andresources of mangrove forests in Batu Ampar,Kubu Raya District, West KalimantanAdi Pasah Kahar♥

Universitas Muhammadiyah Pontianak. Jl Ahmad Yani No.111, WestKalimantan, Indonesia. ♥email: adipasahkahar@gmail.com

Mangrove forests in Batu Ampar, Kubu Raya District,West Kalimantan have many benefits due to its diversitiesof mangrove and other potential resources there. Thisbenefit is the main source of threat to the sustainability ofmangrove in Batu Ampar. The framework of thepreservation of mangrove forests in Batu Ampar needactual and factual information about status of theecosystem as well as the potential resources there.Research uses the method of exploration anddocumentation concerning the condition in mangroveforests. The results show the potential for ecosystems infactor of abiotic components, biodiversity, plant diversity,and resource management activities by the community inthe area of mangrove forests in Batu Ampar. This researchis expected to be utilized by all the people to do planning inthe conservation of Batu Ampar mangrove forest area.

Batu Ampar, mangrove, potential resources

CO-11Growth quality of sandalwood (Santalum album)in forest and plantation of West Timor Island,East Nusa TenggaraYoseph Nahak Seran1,2, ♥, Sudarto3, Luchman Hakim2,Endang Arisoesilaningsih2

1Program of Biology Education, Faculty of Education and Teaching,Universitas Timor, Kefamenanu, Timor Tengah Utara, East NusaTenggara, Indonesia. ♥email: joshseran@gmail.com2Department of Biology, Faculty of Mathematics and Natural Sciences,Universitas Brawijaya. Jl. Veteran, Ketawanggede, Lowokwaru, Malang65145, East Java, IndonesiaDepartment of Soil Science, Faculty of Agriculture, Universitas

Brawijaya. Jl. Veteran, Ketawanggede, Lowokwaru, Malang 65145, EastJava, Indonesia

Sandalwood (Santalum album L.) is a typical plant of theLesser Sunda Island (NTT), grows in deciduous forest or inprivate traditional plantation for centuries. It is, locallyknown as a unity symbol of woody fragrance (haumeni),and economic value are depended on their growth. Thestudy aimed to compare growth quality of trees grown inforest and in plantation in the district of South CentralTimor (TTS) and North Central Timor (TTU). Vegetationanalysis was held by purposive sampling in eight sitescomprised 84 plots. Plots size were 20x20 m2 (trees),10x10 m2 (poles), 5x5 m2 (saplings). Variables observedin each plot included density, stem diameter, height, crownquality and vitality. Data were analyzed by qualitativedescriptive and multivariate statistics. Results showed thattrees growth of sandalwood varied spatially in all studysites and were classified into five groups. Two plantationsin TTS district showed better trees growth qualitycompared to those in TTU. Higher saplings and polesdensity were found in two sites of TTU plantation andforest or a TTS forest. However, higher vitality were foundin a forest of TTS or TTU. Inferior growth was observed inNununamat plantation. Farmers in two districtssuccessfully cultivated sandalwood in plantation shown bysimilar growth quality with those of forest trees.

Forest, growth quality, NTT, plantation, sandalwood

CO-12Stand development in 14-year-old restorationforest on a coal mine site in Central Bengkulu,IndonesiaHery Suhartoyo1,♥, Yanchi Filyadi2, Wiryono2, AliMunawar1

1Department of Forestry, Faculty of Agriculture, Universitas Bengkulu. Jl.W.R. Supratman, Kandang Limun, Bengkulu 38371A , Bengkulu,Indonesia. Tel.: +62-736-21170, psw. 219, Fax. +62-736-21290, email:hery_suhartoyo@yahoo.com2Department of Agrotechnology, Faculty of Agriculture, UniversitasBengkulu. Jl. W.R. Supratman, Kandang Limun, Bengkulu 38371A,Bengkulu, Indonesia

Restoring forest ecosystem on mine sites is still achallenging task for mining operator and the government inIndonesia. Information on long term ecosystemdevelopment on mine sites is still a little. This studyexamines a long term development of restoration forestestablished on coal mine site in Central Bengkulu. We areasking about what are the effect of ripping treatment on thelong term stand development? Is the developed forestecosystem similar to the secondary forest nearby?Measurements were taken in our former Sengon andSungkai stands which was established in year 2000 and insurrounding secondary forest in term of tree diameter, treeheight, understory cover, regeneration potential, soilproperties and environment condition. The result indicatesthat both Sengon and Sungkai stands developed well in a

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former coal mine site. The effect of ripping on diameter ofboth stand were not significantly different, however thestand height showed a significant different. In compare tonearby secondary forest, the afforestation site is nearlyapproaching the condition of secondary forest in term ofsoil properties and environment condition. Considering thisrecent result, better land preparation, especially usingripping treatment, is essential to be practiced for enhancingstand development and forest ecosystem in restoration offormer mining area.

Coal mined, restoration, stand development

CO-13Indirect value of mangrove ecosystems in CoastalDabong, Kubu Raya District, West KalimantanEndang Karlina♥, Vivin S. SihombingForest Research and Development Center, Ministry of Environmental andForestry. Jl. Gunung Batu No. 5. PO Box 165, Bogor 16001, Jawa Barat.Tel. +62-251-8633234; 7520067. Fax. +62-251 8638111, ♥email:endangkarlina88@gmail.com

The mangrove forest ecosystem is a natural resource thathas many functions and benefits in ecology, economy,social aspects and also is an important component ofcoastal ecosystems. The existence of mangrove ecosystemsare not immune to the threat of damage caused byoverharvesting by local communities in the production ofcharcoal, firewood, wood construction. Therefore, theexistence of mangrove ecosystems need to be preserved.The purpose of this study is to : 1) Determine the potentialand diversity of mangrove vegetation; and 2) Carry outeconomic analysis on the indirect benefits and biodiversitybenefits of mangrove ecosystems. In this research, it isused analysis vegetation method and the value of theindirect benefits obtained by the method of economicvaluation. The analysis showed that the discovereddiversity of mangrove flora are Avicennia alba, Bruguieragymnorrhiza, Excoecaria agallocha, Rhizophora apiculata,Sonneratia alba, Xylocarpus granatum and Xylocarpusmoluccensis. The dominant mangrove are Rhizophoraapiculata and Bruguiera gymnorrhiza. The indirectmonetary value of ecosystem in the coastal mangroveDabong as a seawater intrusion barrier are Rp.1.821.600.000, - per year; as the supplier of shrimp feed areRp. 1.272.830.000, - per year and the value of biodiversitybenefits amounts to Rp. 979.002.090, - per year.

Mangrove, indirect value, management

CO-14The estimates of spawning potential ratio of threedemersal dominant species landed in Tegal,Central Java

Duto Nugroho1,2,♥, Mufti P. Patria1, Jatna Supriatna1,Luky Adrianto3

1Postgraduate Program, Department of Biology, Faculty of Mathematicsand Natural Sciences, Universitas Indonesia. Jl. Lingkar Kampus Raya,Kampus UI, Gedung E Lt. 2, Depok 16424, West Java, Indonesia. Tel.:+62-21-7270163 Fax.: +62-21-78849010. ♥email: duto2012@gmail.com2Agency for Marine and Fisheries Research and Development, Ministry ofMarine Affairs and Fisheries, Gedung Balitbang KP II. Jl. Pasir Putih 2Ancol Timur, Jakarta Utara 14430, Jakarta Indonesia3Faculty Fisheries and Marine Science, Institut Pertanian Bogor. Jl. RayaDarmaga, Kampus IPB Darmaga, Bogor 16680, West Java, Indonesia

Java Sea is one of the important fishing grounds in Sundashelf marine ecoregion. It produces approximately 1.5million t/annum of demersal fish species with valued ofUS$2,4 billion dollars. The sustainability of demersalfishery is a crucially concern due to the following reasons:substantial economic contribution, and its significantdependence of small-scale fishers on this fishery for theirlivelihoods. The fishing intensity is considerable andgrowing threats to their habitats. Three dominant species(Priacanthus tayenus, Scolopsis taeniopterus and Upeneussulphureus) were selected in this bio-exploitation study. Toevaluate the vulnerability of these species tooverexploitation, the spawning potential ratio (SPR)approach applied to describe the status of its existingfisheries. The calculated SPR based on the value ofestimated length of first capture (Lc) of each species withreference point of 20%. Across a broad range of species,40% SPR is generally accepted as a proxy for MaximumSustainable Yield (MSY) to sustain their resources. With abaseline of stocks that are generally thought to riskrecruitment declining when SPR < 20%, recent finding onSPR indicated that the existing fishery can be generallydescribed as nearly fully exploited.

Dominant Demersal Species, Java Sea, SPR

CO-15Interferometry SAR (INSAR) data analysis forpeatland and land use monitoring; Case study inKubu Raya, West KalimantanYessy Arvelyna1, ♥, Kazuyo Hirose2, Gusti Anshari3,Tomita Kunihiro1, Hosomi Koichi1, Romi Yanto3

1Civil Engineering & Eco-Technology Consultants. Pontianak, WestKalimantan, Indonesia2 J-Spacesystems, and Center for Wetlands People and Biodiversity.Pontianak, West Kalimantan, Indonesia3Faculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia

We observed peatland areas in Kubu Raya district, WestKalimantan, using a series of ALOS PALSAR image datawith Interferometry analysis of SAR (InSAR). Peatlandtrapped large amounts of carbon and water. Thus thedecreasing of peatlands releases carbon and contributes tothe climate system change. Field survey was carried outduring 2015/8/25-26 which gathered correlation ofinterferometry image and displacement map retrieved fromInSAR processing of each pair of temporal data. Land uses

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of the study area were observed using ASTER opticalimage data and GIS data of peatland and land use map(Ministry of Environment and Forestry Indonesia). Peatsamples were taken and tested at Soil Fertility Laboratory,Universitas Tanjungpura, Pontianak. Organic and ashcontents were analyzed to check decomposition level andestimated using Lost on Ignition (LOI) method at 550oC.We used 16 hours of burning due to large amount oforganic contents. We found that subsided of peatland areaon SAR image has low organic contents, possibly becauseof peat loss and decomposition. Maximum to minimumnegative vertical displacement (subsidence) is observed onpalm plantation, agricultural area and rubber plantation,respectively. The proposed method can be applied toobserve the impacts of agriculture activities and land usechanges on peatlands, and this method is proved toovercome a problem of cloud and haze cover that iscommonly found on Landsat images. Further investigationis needed to determine the mechanism of subsidence andmeasure its impact.

ALOS PALSAR, Indonesia, InSAR, landuse, peatland

CO-16The correlation between environmental factorswith mangrove clam abundance in differentseasons at Segara Anakan Mangrove Forest,Central Java, IndonesiaM. Sofwan Anwari♥

Faculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email: sofwanbio@yahoo.com

Conditions of Segara Anakan (Cilacap Central Java,Indonesia) ecosystems are increasingly damaged. Thiscondition caused decrease the catch of fishermen. For thisreason, they seek alternative fishery resources that can beutilized to meet daily needs. Fisheries resources are widelyused by Segara Anakan people is the mangrove clams. Thisstudy examines the correlation between mangroveenvironmental factors with mangrove clam abundance. Theenvironmental factor include the abiotic (intensity ofsunlight, the light intensity in the shade, air temperature,water temperature, salinity, soil pH, soil organic mattercontent and soil texture) and biotic (vegetation density andchlorophyll content of the water). The research objective isto; 1) assess the abundance of mangrove clam in mangroveforest of Segara Anakan, and 2) analyze the correlationbetween environmental factors and mangrove clamabundance. The survey was conducted in October 2012 andFebruary 2013, the sampling technique on mangrove clamswere done purposively (purposive sampling). Mangrovedamage level is taken into consideration in determining thesampling stations. Analysis of environmental factors thatdetermine the parameters of abundance mangrove clamswith PCA (Principle component analysis). Mangrove clamabundance in the rainy season is higher than the dry season,depend on supply of fresh water that carries nutrients to the

rainy season is greater than the dry season. Based on PCAanalysis, environmental factors that positively havecorrelation on the mangrove clam abundance in the dryseason are water salinity and water content in the soil,while the pH of the soil and the dust content in the soilcorrelates negatively to mangrove clams abundance.Environmental factors that have positive correlation on themangrove clam abundance during the rainy season are soilpH and clay content in the soil, while the sand content inthe soil have negative effect.

Environmental, mangrove clam, seasons

Ethnobiology

DO-01Ethnobotany in Tasik Malaya Village, PadangTikar Island, Kubu Raya District, WestKalimantan, IndonesiaSiti Nur Anisah♥, Entin Daningsih, R. MarlinaProgram of Biology Education, Faculty of Teacher Training andEducation, Universitas Tanjungpura. Jl. Prof. Dr. H. Hadari Nawawi,Pontianak 78124, West Kalimantan, Indonesia. Tel./Fax.: +62-561-740144, ♥email: sitinuranisah25891@gmail.com

This study aimed to invent plant species utilized(ethnobotany) by the Tasik Malaya Village, Padang TikarIsland, Kubu Raya Regency, West Borneo and to know theprocess of its utilization. The research was a qualitativedescriptive method and used triangulation of datacollection techniques such as interviews, observation, anddocumentation. The collection of data sample taken waspurposive sampling. The number of informants was asmany as 43 people representing 10% of the total populationthat was conducted in October-December 2015.The resultsshowed 100 species of plants’ biodiversity were useful for74 species of medicine plants, 8 species for cosmeticsplant, 4 species of dye plants, 9 species of craft plants, and26 species for complement plants in traditional ceremonies.

Biodiversity, ethnobotany, Tasik Malaya Village

DO-02Study of ethnobotanical potency in KalaenaNature Reserve, South SulawesiHadijah Azis Karim♥, ProyantoProgram of Forestry Science, Faculty of Forestry, Universitas AndiDjemma. Jl. Tandi Pau No. 5 Palopo 91914, South Sulawesi, Indonesia.Tel./Fax. +62-471-24506, ♥email hadijahaziskarim@gmail.com,hadijahazis@yahoo.com:

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Kalaena Nature Reserve is a vegetation type of pamahsecondary forest belonging to forest conservation area.However, as existence and interaction between localcommunity and the area are high, it will lead to decrease oreven threatened biodiversity especially plants. In order tosupport the purpose and function of the area, given basicinformation and data for the preparation of the managementplan on Kalaena is needed. This study proposes a researchabout etnobotanical potency through the exploration ofplant species either trees or undergrowth. The research wasconducted in the Kalaena Nature Reserve, MangkutanaDistrict within the Province of South Sulawesi. Data wasobtained through vegetation analysis with purpossivesampling and semi-structured interviews with 30respondents (Snowball sampling). The result of studyshows that there were 65 species having ethnobotanicalpotency to meet daily needs (food, medicines, timber,accessory, customs/religions, decorative plants and other).Betau (Calophyllum euryphyllum), Eboni (Diospyroscelebica) and Kasa (Castanopsis acuminatissima) wereidentified as dominant plants used by local people.Therefore, potencial plants need efforts to cultivate anddevelop in communities-owned land, so that they do not getinto the reserves, and the conservation and preservation ofthe Nature Reserve Kalaena could be maintained.

Ethnobotanical potency, Kalaena Nature Reserve, Pamahsecondary forest, Snowball sampling, Vegetation analysis

DO-03Mangrove conservation ethnobiological toovercome abrasion in Mempawah District of WestKalimantan, IndonesiaLaili Fitria♥, JumiatiFaculty of Engineering, Universitas Tanjungpura. Jl. Jenderal AhmadYani, Bansir Laut, Pontianak Tenggara, Kota Pontianak 78124, WestKalimantan, Indonesia. Tel.: +62-561-36439, email:fitria.laili@gmail.com

Damage to the coastal region, precisely on Sungai Kunyit,due to coastal erosion with an average width rangingbetween 250-1250 m in the next 20 years (Bapelda WestKalimantan, 2003). The impact that is felt by thesurrounding community is the decline in fisheryproduction. Abrasion prevention efforts have been made tocreate a breakwater along the beach. It also conducted amangrove planting, but most have failed. Failure mangroveplanting occurred in several locations in Desa Mendalok -Sungai Duri II. But in Desa Pasir, mangrove planting wassuccessful. Starting from a group of self-awareness and thedangers of erosion, mangrove conservation now in DesaPasir becomes a professionally managed ecotourism. Thepurpose of this study was to analyze the community-basedmangrove conservation in preventing abrasion. The methodused in this study were interviews to village government,founder of mangrove conservation community group, andrural communities. It is known that the necessaryknowledge and observation regarding the planting location,

the supporting substrate, abiotic factors, the suitability ofseeds, and post-planting maintenance. These are things thatare overlooked in the planting activity. Community will notbe procured knowledge of mangrove plants and mangroveecosystems as well as the methods that can be applied inthe planting and post-planting maintenance.

Abrasion, conservation, ethnobiology, mangrove

DP-01Ethnobotanical study of herbal medicine inSebuduh Village, Kembayan District, WestKalimantan, IndonesiaSyamswisna♥, Yokhebed, Hady NugrohoProgram of Biology Education, Faculty of Teacher Training andEducation, Universitas Tanjungpura. Jl. Prof. Dr. H. Hadari Nawawi,Pontianak 78124, West Kalimantan, Indonesia. Tel./Fax.: +62-561-740144, ♥email: syamswisna@gmail.com

West Kalimantan has a wide range of biological diversityof different types of plants that are useful as a medicine.One of the communities which still use plants as medicineis Sebuduh village. The purpose of the study was toinvestigate and collect information from local people on theuse of medicinal plants in Sebuduh Village, KembayanDistrict, West Kalimantan, Indonesia. The field study wasconducted from April-June 2016 through personalinterview, observation, and documentary. The total numberof plant species was 83 species from 48 families used fortraditional medicines among local peoples. The highest useof plants were from 3 families (Zingiberaceae, Asteraceae,and Poaceae). The highest number of plant parts used wereleaves (49 species) and root (17 species) and were followedwith other parts of rhizhome, stem, shoot, fruit, and flower.Herbal medicine had the highest use with boiled, andmashed. Mostly, herbal medicine heal diseases of fever andpostpartum.

Ethnobotany, Kembayan District, medicinal herbs, SebuduhVillage

DP-02Isolation and identification of antiradical andantiphotooxidant compound of Aloe chinensisYohana S. Kusuma Dewi1,♥, Tranggono2, Sri Rahajo2,Puji Hastuti2

1Faculty of Agriculture, Universitas Tanjungpura. Jl. Prof. HadariNawawi, Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-785342, 583865, Fax.: +62-561-765342, email:kusumadewiyohana@gmail.com2Faculty of Agricultural Technology, Universitas Gadjah Mada. Sleman55281, Yogyakarta, Indonesia

A potent antiradical and antiphotooxidant compound fromAloe chinensis was investigated. The results indicated thatthe methanolic extracts produced stronger antiradical and

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antiphotooxidant activity also give higher yields of extractthan other organic solvents. Silica gel column separatedmethanolic extract into five fractions. Of the five fractions,fraction II possessed significant antiradical activity (70.2%inhibition on free radical DPPH) and it showed significantantiphotooxidant activity (60.1% inhibition onhydroperoxide formation). Subsequently, fraction II wasseparated into eight subfractions by silica gel column. Thesub fraction II-b exhibited stronger antiradical andantiphotooxidant activity than others and showed a singlespot in thin layer chromatography Silica gel 60 F254 plates.On the basis of mass spectrophotometry in combinationwith reversed phased high performance chromatography,this compound has been identified as 1, 8 dihydroxy-3-hydroxymetyl anthrone (Aloe emodin anthrone)

Aloe chinensis, antiradical, anti photooxidant

Bioscience

EO-01Hepatoprotective effect of sambiloto leaves(Andrographis paniculata) extract againtsMalondialdehyde levels in liver tissues ofparacetamol-induced wistar rat (Rattusnovergicus)Andriani♥, Guntur Suseno, EffianaDepartment of Microbiology, Faculty of Medicine, UniversitasTanjungpura. Jl. Prof.Hadari Nawawi, Pontianak 78121, WestKalimantan, Indonesia. Tel.: +62-561-765342, 583865, 732500, Fax.:+62-561-765342, ♥email: bundawiam_dr@yahoo.com

N-acetyl-p-benzoquinoneimine (NAPQI) as a product ofmetabolism of paracetamol causes damage to liver functionby causing oxidative stress which is characterized byincreased levels of malondialdehyde (MDA) of liver tissue.Sambiloto (Andrographis paniculata) leaves contain activesubstances, androgapholide and flavonoids that havehepatoprotective effect. The study design was posttest-onlycontrol group. Samples were liver tissues of rats that hadbeen received treatment. A total of 30 rats aged threeweeks were divided into 6 groups, namely the Normalcontrol, Positive Control (curcumin), Negative Control(paracetamol), Dose 1 (500 mg/kg), Dose 2 (1000 mg/kg),Dose 3 (2000 mg/kg) (n = 5). Liver tissue were tested bythe method of Wills. The data was analyzed using One-wayANOVA. MDA levels of liver tissues sequentally; NormalControl (0.338 nmol/mL), Positive Control (0.344nmol/mL), Dose 1 (0.450 nmol/mL), Dose 2 (0.550nmol/mL), Dose 3 (0.634 nmol/mL), Negative Control (1,325 nmol/mL). Post hoc test showed that MDA levels ofliver tissues of Negative Control group was significantlydifferent from other groups. (ANOVA, p≤ 0.05). Therewere no significant differences between MDA levels of

liver tissues of Dose 1 from Normal Control and PositiveControl (ANOVA, p>0.05). it can be concluded that themethanol extract of Andrographis paniculata has ahepatoprotective effect with an effective dose of 500mg/kg.

Androgaphis paniculata, extract of sambiloto, hepatoprotective,MDA

EO-02Comparison of apple’s nutrient content (Malussylvestris) from various sale place in Pontianak,West KalimantanRita Aprillia♥, Entin Daningsih, TitinProgram of Biology Education, Faculty of Teacher Training andEducation, Universitas Tanjungpura. Jl. Prof. Dr. H. Hadari Nawawi,Pontianak 78124, West Kalimantan, Indonesia. Tel./Fax.: +62-561-740144, ♥email: raprillia27@gmail.com

This study aimed to compare the levels of the nutrientcontent of the apple (Malus sylvestris) from various saleplaces in the Pontianak city. The samples were RedDelicious apples, Granny Smith apples and Fuji Sun Moonapples, and taken from three locations, namelysupermarkets, traditional markets, and fruit stores. Nutrientcontent examined were total carbohydrates, glucose,fructose, and sucrose, lipid, protein, water, ash, vitamin Cand crude fiber. Results were analyzed using the SAS 6.12applications with Completely Randomized Design (CRD)Factorial models and significances followed by LSD α =0.05. The results showed that the varieties of applessignificantly affected all of nutrients, sale placesignificantly affected the total carbohydrate, glucose,fructose, sucrose, water, and crude fiber, while thecombination of both significantly affected the totalcarbohydrate, glucose, fructose, sucrose, protein, water,ash, vitamin C and crude fiber. Red Delicious containedmore total carbohydrates, lipid and protein; Granny Smithcontained more vitamin C, crude fiber, water, and ash; andFuji Sun Moon contained more glucose, fructose, sucrose,and water. Apples sold in supermarkets contained morewater, whereas in the fruit store contained morecarbohydrates, glucose, fructose, and sucrose, and apples intraditional market contained more crude fiber.

Malus sylvestris, nutrient content, Pontianak

EO-03The analysis of nutrient and fiber content ofbanana (Musa paradisiaca) which were sold inPontianak City, West KalimantanRizka Hasanah♥, Entin Daningsih, TitinProgram of Biology Education, Faculty of Teacher Training andEducation, Universitas Tanjungpura. Jl. Prof. Dr. H. Hadari Nawawi,Pontianak 78124, West Kalimantan, Indonesia. Tel./Fax.: +62-561-740144, ♥email: sugarrizkah@gmail.com

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This study aimed to find out the effect of varieties ofbanana (Musa paradisiaca) and sale places to nutrients andfiber of bananas which were sold in Pontianak. CompletelyRandomized Design (CRD) Factorial model with mainfactors of varieties of banana (Barangan, Masak Hijau,Singapura), sale places i.e. traditional market, fruit stores,and side road and the combination of varieties and saleplaces of banana. The variable tests were carbohydrate,glucose, fructose, sucrose, protein, lipid, vitamin C, crudefiber, water and ash content test. The result was processedwith SAS application 6.12 version using ANOVA CRDFactorial and significances followed by LSD α=0.05.Result found that varieties of banana affected significantlyon total carbohydrate, glucose, fructose, sucrose, vitaminC, lipid, and water but did not significantly affect on crudefiber, and ash. The sale places did not affected significantlyon total carbohydrate, glucose, fructose, sucrose, protein,vitamin C, crude fiber, water but significantly affected onash content. The combination between varieties and saleplaces affected significantly on specific nutrient content.Barangan was good on total carbohydrate, vitamin C, andash whilst Masak Hijau was highest on glucose, fructose,sucrose, and crude fiber. In addition, Singapura was higheston protein, lipid and water.

Musa paradisiaca, nutrient content, Pontianak

EO-04Proximate analyzes of Tebas Orange (Citrusnobilis var. microcarpa) and Sunkist Orange (C.sinensis) varieties navel and valencia in Pontianak,West KalimantanSandra Welya♥, Entin Daningsih, TitinProgram of Biology Education, Faculty of Teacher Training andEducation, Universitas Tanjungpura. Jl. Prof. Dr. H. Hadari Nawawi,Pontianak 78124, West Kalimantan, Indonesia. Tel./Fax.: +62-561-740144, ♥email: sandrawelya95@gmail.com

This study aimed to measure nutrient content of Tebasorange (Citrus nobilis var. microcarpa) and Sunkist orange(C. sinensis var. navel and var. valencia) sold in Pontianak.The method was Completely Randomized Design (CRD)with Factorial model. The factor consisted of orange withthree varieties i.e. Tebas, Navel, and Valencia orange; thesale places were fruit store and supermarket; andcombination of both. Proximate analyzes were totalcarbohydrate by direct calculate; glucose, fructose, andsucrose content by benedict quantitative; crude fiber bygravimetric analysis; fat content by soxhlet method; proteincontent by spectrophotometry; water content bygravimetric analysis; ash content by direct ashed method;and vitamin C by iodine titration method. Data wereanalyzed by SAS version 6.12 using CDR Factorial modeland significant followed by LSD α=0.05. Results showedvarieties influenced significantly on orange nutrient excepttotal carbohydrate. The sale places of oranges influencedsignificantly on glucose, fructose, sucrose, crude fiber, andvitamin C content, whereas combination of varieties and

sale place influenced significantly on glucose, fructose,sucrose, protein, and vitamin C content with Tebas orangewas the highest on glucose, fructose, and sucrose; Navelorange was the highest on protein and vitamin C based onLSD α=0.05.

Citrus nobilis, Citrus sinensis, nutrient, Pontianak, proximate

EO-05Nutrient content of melon fruit (Cucumis melo)which were sold in Pontianak City, WestKalimantanTina Septiyani♥, Entin Daningsih, TitinProgram of Biology Education, Faculty of Teacher Training andEducation, Universitas Tanjungpura. Jl. Prof. Dr. H. Hadari Nawawi,Pontianak 78124, West Kalimantan, Indonesia. Tel./Fax.: +62-561-740144, ♥email: tinaseptiyani@gmail.com

This study aimed to measure the nutrient content of melon(Cucumis melo) sold in Pontianak city. The study wasconducted with test of total carbohydrate, glucose, fructose,sucrose, crude fiber, protein, fat, vitamin C, water, and ash.Data were analyzed using SAS 6.12 application withCompletely Randomize Design Factorial model andsignificances followed by LSD α = 0.05. Melon varietywere Golden, Orange, and Green melon. Whereas saleplaces were supermarket, fruit and traditional store. Thecombination were melon variety and sale places. Theresults showed that varieties of melon significantly affectedon the total carbohydrate, glucose, fructose, sucrose, crudefiber, protein, vitamin C, and water, but not significantlyaffect on fat and ash. Sale places significantly affected onthe total carbohydrate content, crude fiber, and water, butnot significantly affect on glucose, fructose, sucrose,protein, fat, vitamin C, and ash. The combination betweenfruit varieties and sale place significantly affected on thetotal carbohydrate and water, but not significantly affect onglucose, fructose, sucrose, crude fiber, protein, fat, vitaminC, and ash. Golden melon was good on total carbohydrate,glucose, fructose, sucrose, and protein. Orange melon wasgood on vitamin C and crude fiber. Whilst green melonwas highes in water.

Melon, nutrient content, Pontianak

EO-06Field activities and active flying hours of thebiology pollinator of Apis mellifera honeybees incoffee plantationBudiaman♥

Department of Forestry, Faculty of Forestry, Universitas Hasanuddin. Jl.Perintis Kemerdekaan Km.10, Makassar 90245, South Sulawesi,Indonesia. Tel. +62-411-585917, Fax.: +62-411-585917, ♥email:lintassapi@yahoo.com

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To improve coffee plantation crop production,intensification efforts have been done, the pollinatorsfactors has not received enough attention as a significantpart of intensification. Apis mellifera L honey bees. is abenign and potential as pollinators on coffee plants, butuntil now the field activities and the active flying hourshave not yet known.The aim of this study was to determinethe field activity and the active flying hours of A. Mellifera,the biology pollinator, in coffee plantations. The methodused is survey by placing 20 colonies on the location ofcoffee plantation to observe the behavior of field activitiesand active flight hours, and the data were analyzeddescriptively. The results showed that the field activities ofthe biology pollinator of A. mellifera worker bees was highenough in the dry season (spring coffee), and pollinationactivity tends to increase with increased temperatures,active flying hours of average worker bees ranges from 05:32 until 17: 59 of Indonesia Central Part time with theaverage number of 11.913 active hours per day during therainy season, while during the dry season active flighthours ranges from 05: 20 until 18.50 of Indonesia CentralPart time with the average number of active hours 12.168hours per day.

Active flying hours, Apis mellifera, coffee plantations, fieldactivities

EO-07Physical properties of wood as material ofhandicraft in West Kalimantan with fumigationmethodLolyta Sisillia♥, Farah DibaFaculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email:lolytasisillia@yahoo.co.id

West Kalimantan wood craft was famous and the demandis increasing from foreign country. The craftsman facingthe problem on limited raw material which has highdurability and attractive color. Since Belian (Eusideroxylonzwageri) wood is rare, they used Acacia (Acacia mangiumWilld) and Laban wood (Vitex pubescens Vahl). Efforts toimprove the shelf life and color shades of wood wereconducted with wood fumigation. The study aims todetermine the impact of wood fumigation on raw materialsof wooden craft on physical properties. Timber fumigationtechniques carried out for 12 hour, 24 hour and 36 hour inan chamber with measured 50 cm x 50 cm x 100 cm.Testing standards refers to the method of British Standardincluding wood density, moisture, and thickness swelling.The result of research showed that fumigation improves thedensity of wood, both on Acacia wood and Laban wood.The densities of wood were increase from 0.5237 to 0.6191at 12 hour fumigation; to 0.6601 at 24 hour fumigation andto 0.6417 at 36 hour fumigation respectively. Theproperties of moisture of wood after fumigation processwere better than control wood. The values of wood

moisture of Laban after fumigation were 5.1938% afterfumigation for 12 hour; 7.9352% after fumigation for 24hour and 7.4286% after fumigation for 36 hour. Meanwhilefor Acacia wood, the values of wood moisture were8.1806% after fumigation for 12 hour; 9.1570% afterfumigation for 24 hour and 9.3175% after fumigation for36 hour respectively. The color of fumigated wood wasmore darkening and decorative and easy to craft. Thismethod can be used to increase the properties of wood andperformance of wood.

Acacia mangium, fumigation, Vitex pubescens, West Kalimantan,wood craft

EO-08Characteristic of composite boards made fromsago trunk with treatment different time ofdipping sago trunk and percentage ofcompatibilizerDina Setyawati♥, Farah Diba, NurhaidaFaculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email:dsetyawati007@yahoo.co.id

Composite boards of sago (Metroxylon sago) trunk weremade to overcome the demand problem on wood asfurniture materials. The objective of the research was toevaluate the properties of composite boards from Sagotrunk. The treatment consists of two factors, i.e. dipping thesago trunk in cold water for 24 hour and 48 hour, andadded the compatibilizer Maleic Anhydride withpercentage 2.5% and 5%. Evaluation the composite boardswas according JIS A 5908 2003 standard. The results ofresearch showed that density of composite board rangefrom 0.70 gr/cm3-0.76 gr/cm3.The highest density wasachieved on composite boards from dipping treatment for24 hour without compatibilizer. The average value ofcomposite board moisture content was 2.78% - 4.03%. Thebest moisture content was achieved on composite boardwith dipping treatment for 24 hour and added bycompatibilizer 2.5%. Meanwhile the average value ofthickness swelling was 5.21%.-20.42%. The best result ofthickness swelling was achieved on composite board withdipping treatment for 48 hour and added by compatibilizer5%. The average value of water absorption was 4.36%-24.04% and the best result was achieved from compositeboard with dipping treatment for 24 hours and withoutcompatibilizer. The average value of internal bondingrange between 1.17 kg/cm2 – 5.03 kg/cm2 and the bestresult was achieved from composite board without dippingtreatment and added by compatibilizer 5%. The compositeboards with added compatibilizer MAH 5% have thehighest properties than other composite boards. In term ofefficiency on using compatibilizer, the best result forcomposite boards made from Sago trunk which fulfilled allthe standard of JIS A 5908-2003 was achieved on

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composite board with treatment dipping for 24 hours andadded by compatibilizer MAh 2.5%.

Compatibilizer, composite board, dipping, MAH, sago trunk

EO-09The impact of wood fumigation on rubber woodqualityLolyta Sisillia♥, Dina SetiawatiFaculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email:lolytasisillia@yahoo.co.id

Rubber wood (Hevea brasiliensis) is one of the veryprospective woods for furniture material, handy craft, andother woods product as a substitute for other strong anddurable wood class. For optimum utilities, it is necessary toknow the characteristic of rubber wood, both physics andmechanics. Wood fumigation is an effort to increase woodquality which is used for furniture and craftsmanship. Theobjective of this research is to obtain data physically andmechanically from traditional fumigated rubber wood.Each sampling was took from the base point, middle point,and the tip point from three rubber wood with diameter 30– 35 cm and with free-from-branch length of 2.5 meter.The test of physics characteristic (water content andspecific weight, and dimension alteration) and mechanicscharacteristic (MOE, MOR, and MCS) is based on BritishStandard (BS 373: 1957). The research was usingComplete Random Sampling (RAL). The water content andspecific weight of rubber wood after fumigation treatmentis 2.22% and 0.68. Meanwhile the value of MOE, MOR,and MCS in row after fumigation treatment is: 29670.3kg/cm2, 1484.22 kg/cm2, 154.23 kg/cm2. The traditionalfumigation on rubber wood was able to increase thespecific weight as well as the mechanics characteristic ofrubber wood.

Mechanics characteristic, physics characteristic, rubber wood,wood fumigation

EO-10Water quality of the Pitap river, SouthKalimantanSasi Gendro Sari♥

Department of Biology, Faculty of Mathematics and Natural Sciences,Universitas Lambung Mangkurat. Jl. A. Yani Km. 36, Banjarbaru 70714,Kalimantan Selatan, email: sgsari@unlam.ac.id

The research aimed to determine water quality of Pitapriver in Balangan district. The measurement of waterquality was based on biological-physical-chemicalparameters taken every month with two repetitions onSeptember and October 2014. Five research stationsnamely Ajung, Langkap, Tebing Tinggi, Sungsum and

Juuh were selected using a purposive sampling methodbased on the variety of environment conditions around thePitap river. The results showed that the water quality in theAjung and Langkap villages were classified as not pollutedto little polluted. Other locations in Tebing Tinggi,Sungsum and Juuh villages were determined as polluted.Spearman correlation explained that autotrophic index onSeptember 2014 was closely correlated with the watertemperature and water velocity with it’s value 0.954 and -0.794 at significant value α=0.01. On October 2014 thewater temperature was closely correlated with its value0.681 at significant value α=0.05.

Biological-physical-chemical parameters, Pitap river, waterquality

EO-11Regulatory effects of five medicinal plants used byDayak Uud Danum in West Kalimantan,Indonesia on the delayed-type hypersensitivity andthe inflammation of human colon epithelial cellsYeni Mariani1,2, ♥, Fathul Yusro1,2, Yuko Konishi3,Takahiro Taguchi1, Akira Tominaga1

1Division of Human Health and Medical Science, Graduate School ofKuroshio Science, Kochi University, Okoh-Cho, Kohasu, Nankoku, Kochi783-8505 Japan2Faculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email:yeni.mariani81@gmail.com3 Life and Functional Material Section, Science Research Center, KochiUniversity, Kohasu, Nankoku, Kochi 783-8505 Japan

We evaluated the plants used to treat inflammatory diseasesby Dayak Uud Danum by examining the anti-allergicactivity and the preventive effects on the damage of colonepithelial cells. The plant species examined are Tekeriho(Callicarpa longifolia Lam), Penahan (Myrmeconaucleastrigosa Merr.), Tebelion (Eusideroxylon zwageri Teijsm &Binn.), Kerokak (Scoparia dulcis L.) and Bungur(Lagerstroemia speciosa (L.) Pers.). Anti-inflammatoryactivities of methanol extracts from leaves of these plantswere analyzed in terms of delayed-type hypersensitivityagainst 2, 4, 6-trinitrochlorobenzene using BALB/cAJcmice and in vitro model of intestinal inflammation usingFPCK-1-1 human colon epithelial cells. The yieldpercentage of methanol extract ranges from 4.33 to 8.99%.Extracts from C. longifolia, M. strigosa, E. zwageri, and S.dulcis have suppressed delayed-type hypersensitivity atboth 24 and 48 hours after challenge. These extracts alsosuppressed the migration of eosinophils to the site ofinflammation. L. speciosa extract suppressed the delayed-type hypersensitivity only at 48 hours after challenge. M.strigosa extract showed the preventive effect in the damagemodel of colon epithelial cells by inhibiting the decrease oftransepithelial electrical resistance. Methanol extract of M.strigosa stimulated FPCK1-1 cells to producemucopolysaccharides and that of C. longifolia inducedFPCK1-1 cells to produce IL-22. The production of

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mucopolysaccharides by FPCK-1-1 cells may explain, inpart, the preventive effects of the plant extracts on thedamage of human colon epithelial cells. It is suggested thatleaves from five medicinal plants used by Dayak UudDanum have the potential as anti-inflammatory agents.

Delayed-type hypersensitivity, inflammation, intestinal epithelialcells, medicinal plants

EO-12Effects of medicinal plants in West KalimantanIndonesia to prevent the damage of Human ColonEpithelial FPCK-1-1 Cells and regulate the levelsof blood glucose and triacylglycerol of db/db miceFathul Yusro1,2, ♥, Yeni Mariani1,2, Yuko Konishi3,Takahiro Taguchi4, Mari Tominaga5, Satoshi Kubota4,Akira Tominaga4

1Division of Human Health and Medical Science, Graduate School ofKuroshio Science, Kochi University, Okoh-Cho, Kohasu, Nankoku, Kochi783-8505 Japan2Faculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email: fathulyusro@gmail.com3Life and Functional Material Section, Science Research Center, KochiUniversity, Okoh-cho, Kohasu, Nankoku, Kochi 783-8505, Japan4Division of Human Health and Medical Science, Kochi University,Okoh-cho, Kohasu, Nankoku, Kochi 783-8505, Japan5Department of Medical Technology, Kochi Gakuen College, 292-26Asahitenjin-cho, Kochi, Kochi 780-0955, Japan

The purpose of this study is to analyze the anti-inflammatory and anti-diabetic effects of several plants thatare used by Dayak people to ameliorate diarrhea,stomachache, and diabetes in West Kalimantan, Indonesia.The plants species examined are Durio dulcis, Duriokutejensis, Parkia timoriana, Parkia speciosa,Dracontomelon dao, and Baccaurea costulata. Methanolextracts from wood barks were analyzed in term ofprevention of the damage of FPCK-1-1 human colonepithelial cells and anti-diabetic effects on BKS.Cg-+Leprdb/+Leprdb/Jcl (db/db) mice. Extracts from P.speciosa and D. dao effectively prevented the decrease oftransepithelial electrical resistance of human colonepithelial FPCK-1-1 cells caused by the co-culture withPMA-stimulated THP-1 cells three days after starting theco-culture. Both of these extracts induced FPCK-1-1 cellsto produce mucopolysaccharides. D. dulcis, P. timorianaand P. speciosa effectively decreased the level of bloodglucose of db/db mice in the maltose loading test. Afterfour weeks of oral administration, P. timoriana, P. speciosaand D. dao significantly decreased the level of bloodglucose. Although mice administered with extracts from P.timoriana or P. speciosa consumed less food than thoseadministered with acarbose, there was no significantdifference in body weight among groups four weeks afterstarting administration. D. dulcis and P. speciosasignificantly reduced triacylglycerol. We found thatmethanol extracts from wood barks of D. dulcis, P.timoriana, P. speciosa and D. dao have both activities toprevent the damage of FPCK-1-1 human colon epithelial

cells and down-regulate the level of blood glucose of db/dbmice.

Blood glucose, db/db mice, FPCK-1-1 cells, medicinal plants,triacylglycerol

EO-13Carbon balance in oil palm plantation onpeatland: A simulation of system dynamic modelfor reducing greenhouse gas emissionEvi Gusmayanti1, ♥, Gusti Z. Anshari2, M. Pramulya3

1Department of Agronomy, Faculty of Agriculture, UniversitasTanjungpura. Jl. Prof. Hadari Nawawi, Pontianak 78121, WestKalimantan, Indonesia. Tel.: +62-561-785342, 583865, Fax.: +62-561-765342, email: vie.untan@gmail.com2Department of Soil Science, Faculty of Agriculture, UniversitasTanjungpura. Jl. Prof. Hadari Nawawi, Pontianak 78121, WestKalimantan, Indonesia3Master of Environmental Studies, Graduate Program, UniversitasTanjungpura. Jl. Prof. Hadari Nawawi, Pontianak 78121, WestKalimantan, Indonesia

Rapid expansion of oil palm plantation on peatland hasbeen considered as a major source of greenhouse gasemission. Conversion land into oil palm emits significantamount of carbon released from biomass of previous landuse through either gradually decomposition or burning.Cultivation activities such as lowering or controlling watertable as required for optimum root zone, soil amelioration,fertilizing, or mechanization are factors that also affect CO2emission. In the other hand, these factors determine growthrate and crop productivity. Reducing CO2 emission fromsuch activities would lead to reduction on growth ratewhich imply on decreasing of CO2 uptake forphotosynthesis. The carbon balance between carbonemission and carbon uptake (carbon sequestration) and itsdynamic cycle resulted from alteration a component maybe simulated using system dynamic model. Based on datafrom oil palm plantations in Kubu Raya District, WestKalimantan, we assessed and determined the best scenariofor reducing CO2 emission of oil palm grown on peatland.

Greenhouse gas emission, oil palm, peatland, system dynamicmodel

EO-14Dual interaction between Trichoderma (Localisolates) with Glomus sp. in Acacia mangiumseedlingRosa Suryantini♥, Reine Suci Wulandari, SlametRifanjaniFaculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email: asoerrosa@gmail.com

Trichoderma has known as an fungi deterrent that couldinhibit the growth of pathogen plant and promote plant

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growth. Utilizing of these fungi would be more effective ifthey are isolated from rhizosphere of plants that beinginoculated. Acacia mangium is known to be adaptive butthe growth is highly variated, although symbiosis withmycorrhizae present. Thus Trichoderma innoculation isconsidered necassery to promote the growth of A. mangiumseedlings. The purpose of this research was to obtainTrichoderma (local isolate) which could be synergized withGlomus sp. to promote A. mangium seedlings growth. Toachieve this objectives, the method used were isolation andcharacterization of local isolates of Trichoderma from soilin rhizosphere of A. mangium based on its colonymorphology, and dual inoculation between Trichodermaisolates with Glomus sp. in the seedlings' root of A.mangium. The results showed that there were three isolatesof Trichoderma that have different morphology. The threeisolates (TAK2, TAK3 and TAK5) have morphologicalsimilarity with T. viridae, T. hamantum dan T. virens.Inoculation of Trichoderma isolates showed no significantdifferences on the growth of seedlings. However whenTAK3 was inoculated with Glomus sp., both fungi couldenhance the growth of A. mangium seedlings. This resultindicated that TAK3's isolate also could promote thedevelopment of mycorrhizae in the roots of A. mangiumseedlings.

Acacia mangium, Glomus, seedling, Trichoderma

EO-15Innovation on mechanical and digital biomasscarbon meter for monitoring, reporting andverification (MRV) of forest carbon in WestKalimantanGusti Hardiansyah1,♥, Yudha Arman2, Faurizal2, SigitNormagiat3

1Program of Forestry Science, Faculty of Forestry, UniversitasTanjungpura. Jl. Prof. Hadari Nawawi, Pontianak 78121, WestKalimantan, Indonesia. Tel.: +62-561-765342, 583865, 732500, Fax.:+62-561-765342, ♥email: gusti.hardiansyah@gmail.com2Department of Physics, Faculty of Mathematics and natural Science,Universitas Tanjungpura Pontianak3Department of Agronomy, Faculty of Agriculture, Universitas NahdlatulUlama of West Kalimantan, Indonesia

Universitas Tanjungpura has made the mechanical anddigital device for measuring biomass and tree carbonnamed Untan Biomass and Carbon Meter (UBKM).Mechanical UBKM is a measuring instrument designed tomeasure the concentration of biomass and carbon in a treewithout battery uses, so without charging process, it canimprove the scope and duration of action in the field. Whiledigital UBKM version is created as alternativemeasurement tool that can estimate the content of biomassand carbon stocks in trees in an accurate and efficient way.The method used is an experimental method that includesthe design, manufacture, and testing. The most importantprinciple is the integration of allometric with interpolationdata using the Levenberg-Marquardt method that isattached to a Mechanical UBKM. While the digital UBKM

was based on integration of allometric with a proximitysensor that is processed on the system microcontroller. Thesuccess and calibration will be seen from the test results inthe form diameter, biomass, Carbon and CO2 in accordancewith the table allometric. The second prototype of the toolis expected to be used to determine the content of biomassand carbon stocks to support non-destructive method ofMonitoring, Reporting, Verification (MRV) which is basedon carbon trading REDD + program.

Biomass, carbon, Levenberg-Marquardt, microcontroller,proximity sensor

EO-16The study of system and harvesting pattern ofprivate forest in Jeneberang Watershed, SouthSulawesi, IndonesiaIswara Gautama, Daud Malamassam, Budiaman♥

Department of Forestry, Faculty of Forestry, Universitas Hasanuddin. Jl.Perintis Kemerdekaan Km.10, Makassar 90245, South Sulawesi,Indonesia. Tel. +62-411-585917, Fax.: +62-411-585917, ♥email:lintassapi@yahoo.com

Moreover the specific objective of this research are howmany losses if the opening of the private forest territory(harvesting) of the downstream-upstream area is notcontrolled, how to repair the system and pattern of forestharvesting in the future. This research has been carried outin March to August 2016. The research location is in theGowa, Sinjai and Bantaeng (representing theuppstreamwatershed), whereas Takala, Maros and Makassar(representing the downstream watershed).The results showthat the land area is open due to the harvesting of theprivate forest is around 336.7 ha/year in catchment area ofJeneberang watershed. Harvesting of private forest to theneeds of people still on the verge of safety (JTT still belownormal). Losses (environment valuation) due to theclearing of forest areas (harvesting) to downstreamwatershed Jeneberang (sedimentation) is 109, 027, 040,337 IDR.To achieve the growth rate and teak tree canopythen the coppice system is the best solution in thecontinuity of environmentally-friendly production.

Catchment area, environment valuation, private forests,sedimentation

EO-17Initial moisture content of corncobs plays animportant role in maintaining its quality duringstorageLince Mukkun♥, Herianus J. D. Lalel, YulianaTandirubakFaculty of Agriculture, Universitas Nusa Cendana. Jl. Adi Sucipto Penfui,Kupang 85001, East Nusa Tenggara, Indonesia. Tel.: +62-380-881580.♥email: lincemukkun@yahoo.co.id

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Maize is one of the important staple foods for the people inTimor, East Nusa Tenggara Province. High initial watercontent of the kernel due to improper drying prior storageencountered serious damage and losses during the maizestorage. The purpose of this study was to determine theinitial moisture content that might minimize damage andlosses of maize in the farmer’s storage. The present studyinitiated by drying the harvested corncobs for 0, 2, 4, 6, 8,and 10 days (6 hours a day). This experiment was designedto use Completely Randomized Design with 6 treatmentsand 3 replications. Dried corncobs was stored in thefarmer’s storage for 4 months. The effects of the initialwater content of the maize kernels on the development ofwater content of kernels, the damage percentage of kernels,and the species of pathogen and insects were investigatedduring storage with the 2 weeks interval. The resultsdemonstrated that drying the corncobs prior storage for 10days resulting in 12 percent of water content significantlydecreased the percentage of seed damage to 6, 5%, ascompared to without drying process which resulted in 63%.Aspergillus flavus, Fusarium sp., and Penicillium sp werefound to be the main pathogen during storage. There is noinsect pests was found during the storage.

Insect pest, maize postharvest, moisture content, percentage ofdamage, pathogen

EO-18Effectiveness of Trichoderma for control ofFusarium Wilt of TomatoAgnes V. Simamora♥, Mayavira V. HahulyDepartment of Agrotechnology, Faculty of Agriculture, Universitas NusaCendana. Jl. Adi Sucipto Penfui, Kupang 85001, East Nusa Tenggara,Indonesia. Tel.: +62-380-881580. ♥email: agnes.simamora@yahoo.com

Trichoderma species have long been used as biologicalcontrol agents against plant fungal pathogens includingFusarium oxysporum. The objective of this experiment wasto determine the effectiveness of Trichoderma incontrolling F. oxysporum in vitro and in vivo. Interactionbetween F. oxysporum and Trichoderma in Petri dish assaywas done by placing F. oxysporum and Trichoderma plugs(d= 0.5 cm) on the edge of the medium in a Petri dish. Thedistance of each plug from the edge of Petri dish was 3 cmand the distance between the two plugs was 2 cm. Controlplates contained F. oxysporum grown on media withoutTrichoderma. Cultures were observed daily and percentageinhibition of Trichoderma against F. oxysporum wascalculated. In a greenhouse experiment, F. oxysporuminoculation was done before planting. Trichodermaapplication was done by two ways; i.e soaking the tomatoseeds with Trichoderma suspension or spraying the soilwith Trichoderma suspension at a dose of 100 mL per kgsoil. The percentage of infected seed and disease symptomswere recorded. In both experiments, Trichodermasignificantly inhibited the growth of F. oxysporum.Application of Trichoderma by spraying the soil withspores suspension was significantly more effective in

suppressing F. oxysporum compared to application bysoaking tomato seeds with Trichoderma suspension.

Antagonisms, biocontrol, Fusarium, Trichoderma

EO-19Study of additional cassava Maltodextrin on Aloevera powder makingMaherawati♥, Lucky HartantiFaculty of Agriculture, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-785342,583865, Fax.: +62-561-765342, email:luckyhartantiabubakar@yahoo.co.id

Processing fresh Aloe vera into Aloe vera powder willimproved Aloe vera as functional food and beverage.Maltodextrin made from fresh cassava can be used ascarrier agent on Aloe vera powder to improve efficiencyduring drying processed. The research aims to produceAloe vera powder by the help of maltodextrin from freshcassava as carrier agent. Aloe vera extract and maltodextrinby compatible TSS ratio will be processed on spray dryer(inlet 105°C, and outlet 60°C). Parameter indicators ofAloe vera powder are characteristics of physical anddensity. Aloe vera powder have yield 6.73-7.80%, Aw inthe safe level (0.32-0.38). Browning index 11.43-18.31, itmeans that Maltodextrin added had improved browningindex on Aloe vera powder. Bulk density (0.429-0.463) andTapped density (0.65-0.7 0) showed improving level oncarrier agent added. Carr Index (29.17-38, 98) HausnerRatio (1, 41-1, 64). High of Hausner Ratio mean that Aloevera powder is more cohesive and unfreely flow.

Aloe vera, cassava, maltodextrin, powder

EO-20Lactic acid bacteriaSri Murwani♥, Indah Amalia AmriFaculty of Veterinary Medicine, Universitas Brawijaya. Jl. M.T. HaryonoNo.169, Ketawanggede, Lowokwaru, Kota Malang 65145, East Java,Indonesia. Tel.: +62-341- 2234838, email: dahliatulqosimah@gmail.com

Aflatoxin B1 (AFB1) is a toxic substance produced by thefungus Aspergillus sp which potentially can cause cancer,bring damage to vital organs and even to death. Preventionof aflatoxin poisoning in the host is through a good way offood storage, and certain physical treatments such asheating and radiation but it requires expensive cost, so analternative way to do it is by the use of probiotic bacteria.The study aims to determine the effect of lactic acidbacteria (LAB), namely Lactobacillus bulgaricus againstimmunocompetent cells CD4, CD8 and IL-2 in miceinduced aflatoxin B1. The research method used post testcontrol only design using the 5 treatment groups consistingof: negative control (healthy), positive control (paininduced AFB1), the BAL 7-28 supplementation dose of 1

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mL with a concentration, respectively, (P1) 1x105CFU/mL, (P2) 1x107 CFU/mL and (P3) 1x109 CFU/mL,next all groups of AFB1 is induced by a dose of 0.2 mg/kgorally in 15-28. days. The data collected from cell levelsusing Flow cytometry and is analyzed using One WayANOVA, with α = 0.05 and is continued with Tukey test todetermine differences between treatments. The resultsshowed that the BAL has no effect on the relative levels ofCD4 Th cells but its effect increases in Tc CD8 cells andIL-2 in P2 induced AFB1.

Aflatoxin, immune cell, lactic acid, mice

EO-21Land reclamation on coal mine by using FMAwith intercropping systems in West Sumatra,IndonesiaEti Farda Husin♥, Zelfi Zakir, EddiwalFaculty og Agriculture, Universitas Andalas. Kampus Unand LimauManih, Padang 25163, West Sumatra Indonesia. Tel. +62-751-72773,Fax.: +62-751-72702, email: etifardahusin@yahoo.co.id

As a result of coal mining, environmental damage occurredand yet their efforts to cope with the damage give no goodresult. If the damage is not repaired as soon as possible,there would be disaster for humans. Mining activity beginsby clearing vegetation, excavation subsoil, and coalmaterial and landfill which could result in negativeenvironmental impacts. Attempt to repair the damage to theenvironment needs to be implemented quickly to supportsustainable agricultural development in Indonesia, one ofwhich is to apply natural fertilizers (mycorrhizal fungi) oncrops with intercropping system, such as the aloe plant,corn and cocoa. The study aimed to obtain maximum dosefor each type of plant that produces growth and yield withthe highest production in the coal mining areas in WestSumatra. First year for this research have been donethrough survey method and the application of methods onthe former land of coal mining. Rhizosfir soil samples ofmaize, cocoa and aloes around mined lands are observedunder the microscope on PDA in a petri dish/test tube in alaboratory. From AMF spore identification result, there arenine species spores, namely A. spinosa WV 861A, A.scrobiculata BR984, A. tuberculata VZ 103 E, G.claroideum SC 186, G. etunicatum NE 108 A, G.fistulosum DN 987, SA luteum G. 112, G. versiforme IT104, Glomus sp. From the results of soil analysis, it canalso be seen that the land location of this research containschemical properties and the soil fertility are not good.Mining resulted in tremendous environmental damage.Mining activities are often preceded by land clearing andfelling of vegetation, excavation of soil layers and raw coalmaterials and landfill which could result in negativeimpacts on the environment. it is necessary to apply thestages of reclamation right on land degraded by using pre-reclamation with the implementation of mycorrhizae.

Coal mine, land reclamation, Mycorrhizal Fungi Arbusculla

EO-22Isolation of F protein New Castle Disease Virus ofEast Java wild isolateSri Murwani♥, Dahliatul Qosimah, Indah Amalia Amri,Edy SudjarwoFaculty of Veterinary Medicine, Universitas Brawijaya. Jl. M.T. HaryonoNo.169, Ketawanggede, Lowokwaru, Kota Malang 65145, East Java,Indonesia. Tel.: +62-341- 2234838, email: muratinia@ub.ac.id

Newcastle Disease (ND) nowadays is still a major problemin poultry health, which led to significant economic losses.Vaccination is the only control program, but often there isfailure of vaccination. It is suspected because the antigenicdifferences between vaccine virus with wild isolate. Fromprevious study, it has been understood that wild isolaterepresenting eastern Java velogenic strain ND virus (viruscode: kalidawer), through epidemiological study using Fprotein. Protein F was identified using PCR method withpairs of AA-HL primer (362 bp nucleotides). The F proteinwill be used as material for ND vaccine seed. For thispurpose, it is necessary to understand virus propagation in9-11 day-old embryonated eggs, and to ensure that thevirus is NDV, hemaglutination (HA) and HemaglutinationInhibition (HI) test were done. The identification andisolation of F protein was done by SDS PAGEelectrophoresis. This research obtained ND virus that hasthree major proteins with molecular weights of 80.9 kDa,67 kDa and 41 kDa. The 67 kDa protein was suspected tobe F protein. To ensure that the protein is an F protein,immunoblotting should be performed.

F protein, kalidawer wild isolate, NDV

EO-23Growth evaluation of two different types ofgrafting in three superior varieties of Lai-Durian(Durio zibethinus x kutejensis) from EastKalimantan using different rootstocksWidi Sunaryo1, ♥, Rahman2, Hadi Pranoto1

1Department of Agroecotechnology, Faculty of Agriculture, UniversitasMulawarman. Jl. Pasir Balengkong No.1 Kampus Gunung Kelua,Samarinda, East Kalimantan-Indonesia 75119, Tel./Fax. +62-541-749159/738341, ♥email: widi_sunaryo@yahoo.com2 UPTD Pengawasan dan Sertifikasi Benih Tanaman Pangan danHortikultura, Dinas Pertanian Tanaman Pangan, Propinsi KalimantanTimur, Indonesia. Jl. PM Noor, Samarinda, East Kalimantan.

The most important obstacle of traditional mass-clonalpropagation of superior varieties of Lai-durian (Duriozibethinus x kutejensis) from East Kalimantan is the limitednumber of seeds, since the harvested fruit has only limitednumber of seeds, even seedless. Propagated plant sourcesare by grafting. To overcome this problem, in practices, therootstocks are derived from fruit seeds of other Durio sp.such as Durian (Durio zibethinus) and Lai (Duriokutejensis) that are abundantly available in harvestingseason. This research was performed to evaluate the

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success and growth of two different types of grafting i.e.shoot grafting (Sambung pucuk, in Indonesian) and budgrafting (okulasi, in Indonesian) using three differentrootstock sources (D. zibethinus, D. kutejensis and D.zibethinus x kutejensis). The three national superiorvarieties of Lai-durian from East Kalimantan i.e. LaiKayan, Lai Mandong, and Holai Sentawar were used in thisresearch. The success of grafting is shown throughpercentage and time of successful grafting and the growthof grafted plants measured from the plant height, scionsand rootstock diameter, leaf number, branch numberincrement were evaluated. Surprisingly, the use of D.zibethinus rootstocks resulted in the highest grafted plantgrowth compared to D. kutejensis and D. zibethinus xkutejensis roostcks, although the success of graftingobserved from the percentage and time of successfulgrafting was not significantly different. The otherinteresting result was shown by significant differencesbetween shoot and bud grafting. The success percentage ofshoot grafting (around 94 %) was significantly higher thanbud grafting (around 60 %), but the time required forsuccessful shoot grafting (76 days) was significantly longerthan bud grafting (around 16 days). There were nodifferences of success and growth of grafting among threesuperior varieties of Lai-durian used.

East Kalimantan, grafting, Lai-durian, rootstocks, success andgrowth, superior varieties

EP-01Earthworm and ant for multiplication ofmicorrhizal sporeLaode Muhammad Harjoni Kilowasid1, ♥, La Aris1,Hasbullah Syaf2, La Ode Santiaji Bande3, RachmawatiHasid1, Bahrun1, Halim1, Syamsu Alam1

1Department of Agrotechnology, Faculty of Agriculture, HaluoleoUniversity. Jl. HEA Mokodompit, Anduonohu, Kendari 93231, SoutheastSulawesi, Indonesia. Tel.: +62-401-3191692, Fax: +62-401-3191692,♥email: lohardjoni2@yahoo.co.id2Department of Soil Science, Faculty of Agriculture, Universitas HaluOleo. Jl. HEA Mokodompit, Anduonohu, Kendari 93231, SoutheastSulawesi, Indonesia3Department of Plant Protection, Faculty of Agriculture, Universitas HaluOleo. Jl. HEA Mokodompit, Anduonohu, Kendari 93231, SoutheastSulawesi, Indonesia

Earthworms and ants are categorized into soil ecosystemengineers. The results of activity on soil of both the soilfauna provide suitable microhabitat for mycorrhizal fungi.Objective of this study was to analyze effect of theabundance of earthworms and ants on the mycorrhizalspore population. Experiments carried out in a reactor(FTPE-REACTOR) containing mix cocoa pod huskbiochar and soil for eight weeks. Abundance of earthwormsand ants in each reactor consists of: 0 earthworms and ants,0.5 earthworms and 5 ants, 10 earthworms and 10 ants, and15 earthworms and 15 ants kg soil-1. Biochar levels consistof 0; 50; 100; and 150 g biochar kg soil-1. The number ofspores increased with the abundance of earthworms and

ants, and with time goes by, in all reactors, except reactorscontaining soil and mixture of the biochar and soil whichwithout application of earthworms and ants, number ofspores decreased with increasing biochar level, and theabundance than others was lowest. It was concluded thatthe abundance of earthworms and ants determine thenumber of mycorrhizal spores in the soil. The collaborationof earthworms and ants as ecosystem engineers can asbiotechnology to increase mycorrhizal spores in soil.

Biochar, ecosystem engineers, reactor, soil, spore

EP-02Acute toxicity study of the ethanolic extract ofEleutherine americana leaves in Wistar Rats UsingOECD Guideline 425Robiyanto♥, Sri Wahdaningsih, Eka Kartika UntariDepartment of Pharmacy, Faculty of Medicine, Universitas Tanjungpura.Jl. Prof.Hadari Nawawi, Pontianak 78121, West Kalimantan, Indonesia.Tel.: +62-561-765342, 583865, 732500, Fax.: +62-561-765342, ♥email:robiyant@gmail.com

Introduction. Pre-clinically, the potency of Eleutherineamericana Merr. as antioxidant has been studied, yet thesafety level of this plant is still unknown. Aim. Todetermine the LD50 of the ethanolic extract of E.americana Merr. leaves (EEEaL) in Wistar rats. Method.The acute toxicity test was conducted using OECD 425method (Up and Down Procedure) at two single doses(2000 and 5000 mg/kg BW) of the EEEaL, given orally inWistar rats for two weeks. Results. The phytochemicalscreening test of EEEaL indicated the presence ofalkaloids, flavonoids, triterpenoids, steroids, and saponins.The limit test of dose 2000 and 5000 mg/kg BW of theEEEaL did not show toxic symptoms and did not cause thedeath in rats. The giving of single dose at 5000 mg/kg BWalso did not decrease the body weight until 14 days ofstudy.Conclusion.The LD50 of the EEEaL is more than 5000mg/kg BW. Based on Loomis classification, the category ofthe EEEaL is practically non toxic.

Acute toxicity, Eleutherine americana, leaves, LD50, OECD 425

EP-0Formulation and methods of diagnostic kit storagein stabilizerSayu Putu Yuni Paryati♥, Eka Noneng Nawangsih, IisInayati RakhmatFaculty of Medicine, Universitas Jenderal Achmad Yani. Jl. TerusanJendral Sudirman, Cimahi 40285, West Java, Indonesia. Tel.: +62-22-6652069, ♥email: yunisayu@yahoo.com

This study aims to establish the formulation of anti-idiotype antibody diagnostic kit and to test the stability ofkit in stabilizer and in different pH storage. Diagnostic kit

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is made by utilizing immunoglobulins Y (IgY) and rabbitanti dogs immunoglobulin (IgG). For preparation ofdiagnostics kit, it takes several stages, namely solution A,solution B and prototype of diagnostic kits. Solution A is amixture of S. aureus Cowan I intact in solution Tris HClbuffer with rabbit anti-dog serum with a ratio of 4~6: 2~4(v/v). Solution B is a mixture of anti-rabies idiotype withanti-rabies serum (IgG anti-rabies) which were harvestedfrom a dog with a comparison between the anti-idiotyperabies antigen with anti-rabies IgG is 1~3: 1~3 (v/v).Furthermore, the optimum mix between Solution A and Bwill obtain the prototype of a diagnostic kit that can beused to detect the presence of rabies antibodies in theserum of animals which are suspected of being infectedwith rabies. The optimal conditions of Solution ABdiagnostic kits obtained at a ratio of A: B = 1~3: 1~3 (v/v).Stability kit was tested on several different pH levels,showed that the best activity kit is at pH 7 compared to pH3 and 9. In order to maintain the stability of diagnostic kit,the addition of supplementary materials as a stabilizer withdifferent concentrations were tested, indicating that thehighest activity kit reached by additional of sucrose 5%;followed by glycerin 4% and 1M PBS pH 7.0.

Chicken immunoglobulin, diagnostic kits, formulation, pH,stability

EP-03Isolation and characterization of the antibodyanti-idiotype N-terminal HPV 16 L2 from egg yolkEka Noneng Nawangsih1,♥, Sayu P. Yuni Paryati1, JusufS. Effendy2, Sunarjati Sudigdoadi1, EdhyanaSahiratmadja3

1 Department of Microbiology, Faculty of Medicine, Universitas JenderalAchmad Yani. Jl. Terusan Jendral Sudirman, Cimahi 40285, West Java,Indonesia. Tel.: +62-22-6652069, ♥email: eka.n.nawangsih@gmail.com2 Department of Obstetry and Gynaecology, Hasan Sadikin Hospithal,Bandung, West Java, Indonesia3 Depatment of Biochemistry, Faculty of Medicine, Universitas JenderalAchmad Yani. Jl. Terusan Jendral Sudirman, Cimahi 40285, West Java,Indonesia

One of approaches is the vaccine based on the induction ofantibody responses by anti-idiotypic vaccination. Thisapproach arose from Jerne’s idiotypic network theory,which postulates that B lymphocytes forms a functionalnetwork, with a role in the establishment of the immunerepertoires, in the regulation of natural antibodyproduction. Due to the large potential diversity of theimmunoglobulin variable regions, the idiotypes repertoirecan mimic the universe of self and foreign epitopes. Theaim of this study is isolation and characterization of anti-idiotype N-terminal HPV 16 L2 isolated from egg yolk forseed HPV vaccine. Egg yolk derived from SPF chickenwhich was injected by antibody N-terminal HPV type 16.Egg yolk were purified by solution A and solution B kitand then characterized by SDS PAGE and ELISA. Therewere protein which had molecular weight of 225.000dalton was expected imunoglobulin Y (IgY). Narat (2003)

stated that IgY had molecular weight greater than IgG,which is about 180.000 dalton or greater. Beside that therewere protein which had molecular weight of 95.000 daltonand 49.000 dalton which was expected as Fc dan Fabfragmen from IgY. To find out whether the IgY specific forN-terminal HPV type 16 L2 or no then it is conductedELISA examination. ELISA examination shows thepositive results for specific antibody against anti-idiotypeN-terminal HPV 16 L2. The presence of specific antibodyindicates that the idiotype can mimic the epitop of N-terminal HPV type 16 antigen.

Antibody, anti-idiotype vaccine, characterization, isolation

EP-04Antifertility effect of Lerak (Sapindus rarak) fruitsethanolic extract on female Sprague Dawley ratsInarah Fajriaty♥, Hariyanto I.H., Youngky HaryantoFaculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email:youngkyharyanto109@gmail.com

The objective of this research was to study antifertilityeffect of Sapindus rarak fruits ethanolic extract (SFEE) onFemale Sprague Dawley Rats. Female Rats were dividedinto 4 groups consisting of negative control (KN) groupwhich were given by Carboxymethylcellulose (CMC),positive control (KP) group which were given byEthinylestradiol, dose group 1 which were given with 50mg/kgBW of SFEE, and dose group 2 which were givenwith 100 mg/kg BW of SFEE. All of treatment group weregiven orally on day 0 until 10th day of pregnancy. It gaveresult in a fertility index which was confirmed bylaparotomy on day 20 of pregnancy. Observations onantifertility index include number of corpus luteum,number of implantation, litters born and weight of uterus.The results showed that SFEE dose of 50 mg/kgBB has anantifertility effect on pre-implantation loss at 2.56% andpost-implantation loss at 10.53% and SFEE with a dose of100 mg/kg BB showed the antifertility effects on pre-implantation loss and post-implantation loss respectivelyby 46.15% and 61.54%. This experiment shows that SFEEhas antifertility effect.

Antifertility effect, pre-implantation loss, post-implantation loss,Sapindus rarak fruits ethanolic extract, Sprague Dawley

EP-05Identification antixenosis and antibiosis resistanceof soybean genotypes to armyworm (Spodopteralitura)Ayda Krisnawati♥, M. Muchlish AdieIndonesia Legumes and Tuber Crop Research Institute (ILETRI). Jl. RayaKendalpayak Km 8, PO Box 66 Malang 65101, East Java, Indonesia. Tel.:

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+62-341-801468, 801075, Fax.: +62-341-801496, ♥email:my_ayda@yahoo.com

Armyworm is the main leaf-eating pest in Indonesia. Atotal of 10 soybean genotypes was identified for itsresistance to armyworm through antixenosis and antibiosistests. Genotypes G100H and Ijen were used as resistantcheck due to antixenosis and antibiosis. The experimentwas conducted from April to July 2016 in Laboratory ofBreeding (ILETRI) and Laboratory of Optic (Faculty ofScience and Technology, University of Islam NegeriMaulana Malik Ibrahim Malang). Antibiosis resistance wasmeasured by using preference index, and obtained tworesistant genotype, two moderately resistant, and sixsusceptible genotypes to armyworm. Two resistantgenotypes (G511H/Anjasmoro-1-4 and G100H) tend tohave short and dense trichomme in both of adaxial andabaxial position. Those trichomme orientation becomebarrier for insect pests with chewing and biting type. Basedon armyworm growth, antibiosis resistance was evaluated.Based on antixenosis test, two resistant genotypes showeda lighter larvae and pupae weight, and also have a longerstadia of larvae and pupae than the susceptible genotypes.Furthermore, the pupae weight seem to be more influentialthan the weight of larvae due to the mechanism ofantibiosis. Hence, genotypes G511H/Anjasmoro-1-4 andG100H can be used as source of genes for improvement ofsoybean resistance to armyworm.

Antibiosis, antixenosis, armyworm, soybean

EP-06Effect of enzyme combination (polygalacturonase,cellulase and amylase) on keprok garut (Citrusnobilis var. chrysocarpa) in juice purificationEsti Widowati♥, MAM. Riani, Cahyo Bagus SulistyoDepartment of Food Science and Technology, Faculty of Agriculture,Universitas Sebelas Maret. Jl. Ir. Sutami 36 A Kentingan Jebres Surakarta57126, Central Java, Indonesia. ♥email: esti_widowati@yahoo.com

The purpose of this study was to determine the changes ofviscosity parameter, transmittance, pH, and total solublesolids (TSS) on keprok garut in juice purification withcombination of pectinase, cellulase, and amylase enzyme.Three bacteria isolates producing the enzyme arepectinolytic bacterial isolate AR 9, cellulolitic bacteriaisolate S 6, and amylolytic bacteria isolate K 8 will be usedfor the production of polygalacturonase, cellulase, andamylase enzyme. The experimental design used in thisresearch was Completely Randomized Design with onefactor. The juice sample is analyzed by three replicationsamples and two replication analysis. The data analyzedwith SPSS 16.0 software using analysis of variance(ANOVA), if there is a significant difference, then it willbe continued with Duncan Multiple Range Test (DMRT) atsignificance level of 0.05. The results showed that thecombined enzymes of pectinase, cellulase, and amylase onPacitan orange in juice purification can lower the viscosityvalue of the control from 1,4028 cP into 1,2192 cP, canlower the TSS value of the control from 11,9 be 11,0° Brix,can increase the pH value of the control from 3,23 to 3,26,and can increase the transmittance value of the controlfrom 1,0% to 1,3%

Amylase, cellulase, clarification, enzymes combination, keprokgarut orange, polygalacturonase