international multidisciplinary research conference on

International Multidisciplinary Research Conference on

Biodiversity, Climate Change, Environment and Life Sciences

Venue: S.B.E.S. College of Science, Aurangabad, Maharashtra

29-30 July 2019

ISBN: 978-93-5382-353-5

Organised and Hosted by:

S.B.E.S. College of Science, Aurangabad, Maharashtra, India

In Association with:

International Academy of Science and Research, KolkataWest Bengal, India

In Collaboration with:

Confederation of Indian Universities (CIU), New Delhi

BOOK OF ABSTRACTS

First Edition: July 2019

Copyright: International Academy of Science and Research, Kolkata

ISBN: 978-93-5382-353-5

Price: INR 100

DISCLAIMERThe authors are solely responsible for the contents of the abstracts and papers compiled in this book. The publisher or editors do not take any responsibility for the same in any manner. Errors, if any, are purely unintentional and readers are request-ed to communicate such errors to the editors or publisher to avoid discrepancies in future.

Published By: International Academy of Science and Research42, Station Road, Kolkata, West Bengal

Composed and setting by: Binary Banyan Uttarayan, Sodepur, Kolkata- 700110

Organising CommitteePATRONS:Chancellor (Dr.) P R Trivedi, President, Confederation of Indian Universities (CIU), New DelhiShri Ram Bhogle, President, S.B.Education Society, AurangabadDr. Amit Krishna De, Executive Secretary, Indian Science Congress Association, Govt. of IndiaDr. Nandkumar Ukadgaonkar, General Secretary, S.B.Education Society, AurangabadDr. Tridib Bandopadhyay, Chairman, International Benevolent Research Foundation, KolkataDr. Ulhas Shiurkar, Joint Secretary & Chairman of CDC, S.B.E.S.College of Science, Aurangabad

PRESIDENT:Dr. Sudip Barat, President, International Academy of Science and Research (IASR), KolkataJOINT CONVENORS:Dr. P. V. Jabde, Principal, S.B.E.S. College of Science, AurangabadDr. Tanmoy Rudra, Secretary, International Academy of Science and Research (IASR), KolkataORGANIZING SECRETARY:Dr. Pramod Davne, Head, Dept.of Zoology, S.B.E.S.College of Science, Aurangabad

NATIONAL ADVISORY COMMITTEE:Dr. S. S. PatilDr. Suresh GaikwadDr. Durgapada KarmakarDr. Arun VedpathakDr. Arvind DhabeDr. Pravin WakteProf. G.K.KulkarniProf. Y. K. KhillareDr. Anil Pardeshi

Dr. D. L. SonawaneDr. A K VermaDr. Ravi ReddyDr. J. V. JagatkarDr. Ashok MoteDr. T. J. JadhavDr. Pradeep Braamhapurikar,Dr. Mohammad IlyasDr. Aniruddha Ray

ORGANIZING COMMITTEE:Dr. K. S. KhobragadeDr. D. D. KayandeDr. D. T. PatilDr. A. G.ShankarwarDr. S. V. VaidyaDr. A. S. NaikDr. P. M. DhakneProf. Dhanraj KambleDr. V. V. Deshpande

Dr. A. B. DasputeDr. R. D. MadhekarDr. P. R. PardeshiDr. C. V. Sirsath, VaijapurDr. Vidya PradhanDr. S. S. ShindeDr. D. D. Khamkar, BadnapurDr. Aniruddha Budrukkar, GeoraiDr. J. D. Shaikh

Dr. V. R. MoreDr. D. M. PathanDr. Ravi Pradhan, SeluDr.Laxmikant Shinde, Jalna.Dr. V. B. Sakhare, AmbajogaiDr. S. V. Saraf, PaithanDr. S. B. Ubarhande, PathriDr. S. T. Naphade, SillodDr. R. K. Barote, Soygaon

International Academy of Science and Research (IASR)48, Station Road, Rahara, Kolkata- 700118, West Bengal

Mobile/ WhatsApp: 9831446832 Email: [email protected]

Website: www.iasr.ind.inIntroduction: International Academy of Science and Research (IASR) has been established on the auspicious occasion of the National Science Day on 28th February 2015 based on its incorporation as a Charity under the Central Act II of 1882, Government of India in Kolkata with the main objective of locating avenues for alternative employment creation besides designing a neological as well as neocratic approach to research and entrepreneurship in the field of Science and research among the younger generation all over the world in general and India in particular with a view to bringing peace on earth in the third millennium by having country wise appropriate solutions for the burning problems like peacelessness, poverty, greed, unemployment, faulty educational, training and research methodologies, religious intolerance, etc., The International Academy of Science and Research (IASR) has decided to design a master plan paradigm (2016-2025) for a new world order. The activities of IASR will include the strategies for creating more researchers besides transfer of appropriate technologies among the globe for ensuring a balanced and a sustainable growth in all countries of the world by using clean as well as cleaning-up technologies through new and emerging techniques for climate change management, environmental and disaster education, geriatric care, waste management, green business and technologies besides strengthening of diplomatic relations among nations for protecting our Mother Earth.The idea is also to promote entrepreneurial educational leadership among the school and the college going boys and girls by “Catching Them Young” and for designing appropriate messages for the educators to see that they produce a greater number of job givers rather than job seekers. This will be possible as IASR has the qualified inventory of experts for establishing universities, colleges, institutions, schools and other training enterprises in different countries with the latest equipment and infrastructure for conducting formal, informal, non-formal, open, distance, online, internet and webbased employment-centric programmes in all countries of the world. IASR encourages inclusive social development through the spread of universal quality education and supports the involvement of communities and citizens in the endeavour.

Areas of Activities: • Scientific, Social & Industrial Research • Agriculture, Horticulture and Forestry Sciences • Animal, Veterinary and Fishery Sciences • Anthropological and Behavioural Sciences (including Archaeology, Psychology, Education,

Counselling, Psychotherapy etc.) • Biological Sciences (including Botany, Zoology, Biotechnology, Biophysics, Genetics,

Molecular Biology, Bioinformatics, Applied Biology etc.) • Chemical Sciences (including Biochemistry, Nano Science etc.) • Computer and Information Technology • Earth and Geological Sciences (including GIS and Remote Sensing, Sustainable Development,

Population and Habitat Studies etc.) • Engineering Sciences • Environmental Sciences • Judicial Sciences (including Law, Criminology. Forensic science etc.) • Library and Information Science • Material Science • Medical and Paramedical Sciences (including Optometry, Medical Laboratory Technology etc.) • Management Science • Mathematical and Statistical Sciences • Marine Sciences • Pharmaceutical Sciences • Physical Sciences (including Atmospheric Sciences etc.) • Social Sciences (including Geography, Economics, Political Sciences, Human Rights,

Journalism and Mass Communication, Rural Development. Sociology etc.) • Sports Sciences

Awards of IASR: IASR recognizes the contribution of scientists, academicians, researchers in the different field of science research and education. The Institute has instituted several awards to motivate and further the spirit of the talented ones in the field of science research and education. The selection of the awards is considered mainly on the basis of the merit.

(i) Scientist of the Year Award (Above 45 Years of age) (ii) Young Scientist of the Year award (32-45 years of age) (iii) Junior Scientist of the Year Award (Below 32 years of age) (iv) World Award for Research and Development (v) Science Excellence Award (vi) Social Science Excellence Award (vii) Lifetime Achievement Award for Research/ Teaching (above 60 years of age)

For further details mail to: [email protected] or Call/ WhatsApp on 9831446832

Membership of IASR: IASR provides a platform where top Academicians, Researchers, Industrialists, Functional Heads, Managers, Bureaucrats and others come together to share knowledge and experience. All members of IASR, will have access to exclusive member benefits, some with free or discount rates. The value of IASR membership is far more than the cost of membership. We request you to strengthen the Education Fraternity by enrolling as a valued member of IASR and leverage from IASR activities, contributing a bit of yours that can make a big difference to the Education Community at large.Graduation is essential for membership.Student Membership Fee: Rs. 1000 only (Up to Master Degree Level) Life Time Membership Fee: Rs. 3000 only Fellowship Fee: Rs. 7000 only* (Presently close)

*Life membership with Ph.D is essential to become the fellow member. Fellow members are entitled to suffix FIASR after their name.

For membership mail to: [email protected]

Communication Address: Dr. Tanmoy Rudra, General Secretary International Academy of Science and Research 48, Station Road, Rahara, Kolkata- 700118, West Bengal, INDIA Mobile / WhatsApp: 9831446832

Email: [email protected]

S.B.E.S. College of Science, Aurangabad, MaharashtraS.B.E.S.College of Science, Aurangabad, venue of this International Conference is run by Shri Saraswati Bhuvan Education Society, Aurangabad. The Society was established in the year 1915 and recently celebrated its 100 years. The institute is committed to social and educational reforms. It has provided educational facilities in Marathi to the students of the region, especially girl students, during Pre-Independence days and continues the mission till today. College was established in 1963 and from the beginning the institution has blossomed into one of the leading science college in this region. Our college celebrated 2013-14 as Golden Jublee Year. NAAC awarded “Four” star status to the college in the first accreditation in November 2001 and in 2014, college was reaccredited with ‘A’ grade. The college is having Undergraduate (B.Sc) as well as Postgraduate (M.Sc.) courses in Environmental Science, Computer Science, Information Technology, Organic Chemistry, Analytical Chemistry and Electronics, B.Voc in four subjects with 12 add on courses. A special facility of Central Research Laboratory is available for researchers and recognized teachers for doctoral work.

Confederation of Indian Universities (CIU), New DelhiAs we approach the Twenty first Century, a number of major challenges face women and men around the world as they interact with one another as individuals, groups, and with nature. Globalisation of trade, of production, and of communications has created a highly interconnected world. Yet the tremendous gaps between the rich and the poor continue to widen both within, and between nations. Sustainable development remains an elusive long-term goal, too often sacrificed for short-term gains. It is imperative that higher education offer solutions to existing problems and innovate to avoid problems in the future. Whether in the economic, political, or social realms, higher education is expected to contribute to raising the overall quality of life, worldwide. To fulfil its role effectively and maintain excellence, higher education must become far more internationalized; it must integrate an international and intercultural dimension into its teaching, research, and service functions. The most significant feature of education for mother earth protection in the 21st century is not so much what the French call li explosion scolarie, but the knowledge explosion, which has expanded the catchment areas of learning so fast that it takes only a few years now for the state-of-the-art in any field to become obsolete. Different modes and types of communicating for advancement of knowledge are fast changing and becoming more than sophisticated. In this technological era knowledge can easily be dispensed technologically and electronically. Teachers and formal school structures are becoming less important, and the conventional age limits on the learning process are becoming blurred. Viewing the urgent need for mutual and technical cooperation among the Universities in India, exchange of information, export and import of educational know-how and consultancy, control on duplication of efforts and wastage in higher education, vocationalisation of existing careers besides strengthening the financial health of the existing Universities for implementing educational programmes having social, cultural, technical, economic and positive contents for the optimum development of our country, the “Confederation of Indian Universities (CIU)” has been established with the co-sponsorship of selected university level institutions in India.

ISBN: 978-93-5382-353-5

International Multidisciplinary Research Conference on Biodiversity, Climate Change, Environment and Life Sciences

Page| 9

GROUP- ABiodiversity and Climate Change

ISBN: 978-93-5382-353-5

S. B. E. S. College of Science, Aurangabad, Maharashtra, India ▪ July 29-30, 2019

Page| 10

ISBN: 978-93-5382-353-5


Page| 11

Environment, Climate Change & Challenges for FisheriesAcharya K.V.1, Shembekar V. 2, Korde S.3, Pethkar M. 4, Jadhav S. 5

1Swami Ramanand Teerth Marathwada University, Nanded Maharashtra2Dept. of Zoology and Fishery science, R.S.M. Latur

3Azad College, Ausa Dist: Latur Maharashtra4Dayanand Science College, Latur.Maharashtra

5Baburao Patil College of Arts & Science, Angar, Solapur

Abstract: India’s fisheries resources are rich and diverse as they are plentiful, comprising ocean, rivers, floodplains, estuaries, mangroves, estuarine impoundments, lagoons, upland lakes, reser-voirs and ponds.India has blessed with Rivers 290000 km, Reservoirs 3.15 million ha, Tanks & ponds 2.35 million ha. Estuaries 0.29million ha. Mangroves 0.7 million ha. Backwater & lagoon 0.9 million ha. Floodplain wet lands 0.2 million ha. Upland lakes 0.72 million ha. Major rivers 14 & Minor rivers are 44. (Handbook of fisheries & aquaculture, 2011). With its 8118 km long coast-line, 2.02 million sq. km (EEZ) Exclusive EconomicZone (EEZ act 1976).In 2013-14 India is the second largest producer of fish in the world contributing to 5.68% of global fish production as per FAO statistics. India is a major producer of fish through aquaculture and ranks second in the world after China.Climate change is predicted to have a range of direct and indirect impacts on marine and freshwater capture fisheries, with implications for fisheries-dependent economies, coastal com-munities and fisher folk.Fisheries and fisher folk may be impacted in a wide range of ways due to climate change. These include biophysical impacts on the distribution or productivity of marine and freshwater fish stocks through processes such as ocean acidification, habitat damage, changes in oceanography, disruption to precipitation and freshwater availability. Fisheries will also be exposed to a diverse range of direct and indirect climate impacts, including displacement and migration of human populations, impacts on coastal communities and infrastructure due to sea level rise and changes in the frequency, distribution or intensityof tropical storms.In marine regions model pro-jections suggest decreases in maximum catch potential in the world’s exclusive economic zones of between 2.8 percent and 5.3 percent by 2050 according to greenhouse gas emission scenario RCP (Representative Concentration Pathway) 2.6, and between 7.0 percent and 12.1 percent according to greenhouse gas emission scenario RCP 8.5, also by 2050(IPCC fifth Assessment Report (AR5) in 2014). In view of the above, the present paper would definitely throw some light on these pre-dicted impacts, and introduces and applies the concepts of vulnerability, adaptation and adaptive capacity.Paper highlights the multifaceted and interconnected complexity of fisheries and aquacul-ture, through which direct and indirect impacts of climate change will materialize. Efforts to adapt to and mitigate climate change should be planned and implemented with full consideration of this complexity.Paper is acts as a reminder of the critical importance of fisheries and aquaculture for millions of people struggling to maintain reasonable livelihoods through this sector.Keywords: Fisheries resources, Environment, Climate Change, Climate change impacts fisheries and aquaculture

ISBN: 978-93-5382-353-5


Page| 12

Critical Impacts of Global Warming on Planet EarthDeepak Devilal Pardhe

Department of Botany, Sant Dnyaneshwar Mahavidyalaya, Aurangabad

Abstract: Natural resources offer the foundation for life on earth. Natural resources are referred to the naturally occurring substances which are considered precious in their relatively unmodified condition. One of the biggest problems the world facing today is global warming. Many experts be-lieve that our production of carbon dioxide and other greenhouse gases are heating the atmosphere and this could be very dangerous for living being on planet earth. Globally increasing temperature is likely to have impacts on terrestrial, aquatic and marine ecosystems that are difficult to manage. Changes in temperature change the great patterns of wind that bring the monsoons in Asia, rain and snow around the world, making drought and unpredictable weather more common. Global warm-ing is projected to have a number of effects on the oceans which include rising sea levels due to thermal expansion and melting of glaciers and ice sheets and warming of the ocean surface, leading to increased temperature stratification. In the geologic past, natural climate changes due to glob-al warming have caused large-scale geographical shifts in species ranges, changes in the species composition of biological communities and species extinctions. Changes in species composition will have large effects on local and regional economies and biological diversity. Climate change has brought about possibly permanent alterations to Earth’s geological, biological and ecological systems. These changes have led to the emergence of large-scale environmental hazards to human health such as extreme weather, ozone depletion, increased danger of wild land fires, loss of biodi-versity, stresses to food producing systems and the global spread of infectious diseases.Major reason behind global warming is emission of greenhouse gases like Carbon Dioxide, Meth-ane, Nitrogen Oxides and Fluorinated gases. As an individual it is our responsibility to control global warming. We must plant maximum number of trees as they releases oxygen and absorbs carbon dioxide present in atmosphere. In this way a tree balance the temperature of air and reduces the amount of carbon dioxide present in air. The vehicles release many harmful gases in the air. Hence, try to drive those cars which run on gas or electricity. If possible minimize the use of per-sonal vehicle and travel by public transport. This way we can also control the problem of pollution and ultimately global warming.Keywords: Global warming, Greenhouse gases, Earth, Responsibilities

ISBN: 978-93-5382-353-5


Page| 13

Study of Diversity of Snakes Found in and Around Dhanlaxmi Nagar, Parbhani, Maharashtra

Hema D. MakneDeaprtment of Zoology, B. Raghunath A.C.S. College, Parbhani

Abstract: Snakes are abundant all over the world. Snakes are intrinsically fascinatory and form an important component of the food chain They play key ecological roles in controlling rodent pests, they maintain the nature and serve a lot to mankind. Deflection of these animals throughout to globe and their extinction causing a continuous and diligent task to the people of all spheres of the soci-ety to conserve them. A checklist of snakes is prepared from Dhanlakshmi nagar, Parbhani on the basis of the information collected from the survey during August 2017 to Feb 2018.The captured snakes represent 12 types of species under 5 families. In these families 5 were venomous snakes, 6 nonvenomous snakes. The present study is an attempt to evaluate the information, occurrence, abundance &species richness and further assist in the knowledge, awareness and conservation of snake fauna in this region. So this study may play helpful role in conversation of the biodiversity.Keywords: Snake, biodiversity, Venomous, Parbhani

Biodiversity and Seasonal Variation of Ciliates (Polymenophorea) from Perennial Water Dody in Waluj near Aurangabad, Maharashtra

Rafat NahriSir Sayyed College, Aurangabad

Abstract: Studies on freshwater free living protozoa has been proved to be important in their applications as bioindicator species and defining the climatic conditions. Biodiversity of Ciliates (Polymenophorea), free living freshwater protozoa has been studied from perennial water body near Aurangabad.It is an attempt to co-relate the seasonal fluctuations in population density of ciliates (Polymeno-phorea) with some selected physico chemical parameters, seasonwise and yearwise. The parame-ters studied were atmospheric temperature , water temperature, humidity, rainfall, pH, D.O, acidity, total alkalinity, hardness, total nitrogen, chloride, phosphate and total solids . The different species recorded from this major group of ciliates, polymenophorea were Halteria grandinella, Euplotes affinis and Euplotes patella. Keywords: Perennial waterbody, ciliates, seasonal variation

ISBN: 978-93-5382-353-5


Page| 14

Qualitative Phytochemical Screening of Some Selected Medicinal Plants of Saptashrungi Hill of Nashik District, Maharashtra

M. R. Bachhav1 and V. K. Wahule2

1Dept. of Chemistry and 2Dept. of ZoologyKarmveer Ramraoji Aher Art’s, Science and Comm. College, Deola, Nashik

Abstract: The use of herbal medicines is on trial and effect basis by the tribal’s. This knowledge is transmitted orally and not recorded by the medicine men. It is need to convert into documentation form for future generations. Herbal treatment is a cheapest and easiest method of treatment to cure various diseases. Medicinal plants are easily available, cheaper in cost, no any side effect and per-manent effect, hence now a day people turns to use of herbal treatment. World have rich heritage of herbal medicines since several centuries ago. At present 7500 species of plants are used for medic-inal purpose in India (Natesh and Mohan Ram, 1999).In the present study survey of medicinal plants, their distribution, taxonomy and preliminary chem-ical analysis of selected economical medicinal plant have been carried out during 2017 to 2019 at Saptashrungi hill of Taluka Kalwan District Nashik. During this period regular visits were arranged to study area. In this area we have observed many medicinal plants. We have interaction with the medicine men or Vaidus and collected information on ethno medicinal plants. Out of these plants we have selected only four uncommon medicinal plants from study region for further detail study. The selected medicinal plants were identified with the help of Floras (Naik, 1998, T. Cook, 1958) and experts. Selected medicinal plants occurrence, distribution, morphological study and uses have been carried out (Chopra R.N. et. al 1956). Keywords: Saptashrungi hill, phytochemical screening, Herbal treatment, Medicinal plants

Global Warming: Increasingly Noticeable ImpactsJyoti Basavant Hattarage and Rathod G. T.

Jawahar Arts, Science and Commerce College, Andur, Osmanabad

Abstract: Global warming is a long-term trend, but that does not mean that every year will be warmer than the previous one. Day to day and year to year changes in weather patterns will contin-ue to produce some unusually cold days and nights, and winters and summers, even as the climate warms. Climate change means not only changes in globally averaged surface temperature, but also changes in atmospheric circulation, in the size and patterns of natural climate variations, and in lo-cal weather. Stronger winds from Polar Regions can contribute to an occasional colder winter. In a similar way, the persistence of one phase of an atmospheric circulation pattern known as the North Atlantic Oscillation has contributed to several recent cold winters in Europe, eastern North Ameri-ca, and northern Asia. Atmospheric and ocean circulation patterns will evolve as Earth warms and will influence storm tracks and many other aspects of the weather. Global warming tilts the odds in favor of more warm days and seasons and fewer cold days and seasons.

ISBN: 978-93-5382-353-5


Page| 15

The Study of Biodiversity in Achaler Water TankJyoti Basavant Hattarage and Rathod G. T.

Jawahar Arts, Science and Commerce College, Andur, Osmanabad

Abstract: The present study deals with the biodiversical analysis of water tank located in a place called Achaler. The species of fresh water algae have been recorded from Achaler tank. The spe-cies was belonged to Cyanophyceae, Chlorophyceae, Bacillariophyceae, and Euglenophyceae. As per heamocytometer cell count study, the maximum number was recorded in Cyanophyceae i.e. Microcystis aeruginosa 3,11,700 /ml. Minimum cell number was recorded in Chlorella sp. and Pleurosigma angulatum 450/ml. Since the BOD and the essential nutrients are in appreciable amounts in the pond, it is very clear that this temple tank is undergoing eutrophication. As per the results of Nygaard’s index indicating the trophic level of the water bodies the temple tank shows eutrophic state. This is because of the presence of desmids such as Closterium sp, Cosmarium sp and Staurastrum sp. in the tank. The abundance and biodiversity of the algal forms indicate the eutrophic nature of the water body.Keywords: Achelar, algae, BOD, Biodiversity, Tank water

Impacts of Biodiversity Loss on HumanityKhole A. M.1 and Gaikwad J. M.2

1B. Raghunath ACS College, Parbhani 2Shri Shivaji College, Parbhani

Abstract: Globalization population, industrialization, urbanization, geopolitical problems, accel-erate global change around the world and are in turn leading to a significant erosion of in turn leading to a significant degradation of biodiversity, significant degradation of ecosystems and large number of migratory movements, both by human and animal populations on large scale. Around the world due to an increased rate of globalization and various global changes, human beings are now facing the problems of spread of infectious agent, rising risks of pandemics and many chronic non-infectious diseases. These changes highlight the globalization of health risks, as well as the im-portance of the human-animal-ecosystem. Humans have relied historically on nature for obtaining a wide variety of nature for obtaining a wide variety of natural resources that have been an integral part of our economic systems. Biological diversity is a key component of ecosystems which is re-sponsible for the direct and indirect provision of several benefits that humans derive from nature.Keywords: Biodiversity, globalization, diseases, resources

ISBN: 978-93-5382-353-5


Page| 16

Invasive Alien Species: Serious Threat for Sustainable BiodiversityW.A.H.P. Guruge

Department of Zoology, University of Ruhuna, Matara, Sri Lanka

Abstract: Convention on Biological Diversity defines an Invasive Alien Species (IAS) as species native to one area or region, that have been introduced into an area outside their normal distri-bution, either by accident or on purpose, and which have colonized or invaded their new home, threatening biological diversity, ecosystems and habitats, and human well-being. IAS are the major direct driver and second biggest threat to biodiversity after habitat destruction and fragmentation. (IAS) have resulted in major impacts on biodiversity at a global scale, where at least39 per cent of the species extinctions during the past 400 years are due to IAS (www.indiaenvironmentportal.org.in/node/38152). IASoccur in all taxonomic groups, including: animals, plants, fungi, microor-ganisms. The sequential phases in the invasion are Introduction of the species, Establishment and reproduction of the introduced species, and Spread. Globalization has resulted in greater trade, transport, travel and tourism, all of which can facilitate the introduction and spread of IAS. Human activities have dramatically increased opportunities for species to travel around the world and to become invasive.The World Conservation Union, states that the impacts of IAS are immense, insidious, and usually irreversible. They may be as damaging to native species and ecosystems on a global scale as the loss and degradation of habitats. Invasive alien species can also lead to changes in the structure and composition of ecosystems leading to significant detrimental impacts to ecosystem services, affect-ing economies and human wellbeing. For humans, one of the most dangerous effects of IAS is as a carrier of disease. The Asian tiger mosquito has been linked to more than 20 diseases, including yellow fever and chikungunya fever.Considering remedial measures, Prevention is the most cost-efficient and effective method against IAS. Another important measure is developing economic tools and incentives for the prevention of introductions, educate and aware the general public about IAS.To combat IAS introduction, Sri Lanka has initiated the National Early Warning System for In-vasive Alien Species (Pre- entry Risk Assessment). It is a scientifically developed methodology which can be used to assess the invasiveness of non-native species before the introduction of such species or at the early stage of introduction. Through using this tool, Risks associated with IAS in Sri Lanka were assessed under 4 categories i.e. ecological and economic impacts, invasive poten-tial, distribution and management aspects of invasive species.Since IAS are a universal issue, the collaboration among governments, economic sectors and non-governmental as well as international organizations is inevitable.Keywords: Convention, Establishment, Globalization, Remedial measures

ISBN: 978-93-5382-353-5


Page| 17

Impact of Human Interventions on Zooplankton Diversity in Accor-dance with Physico-Chemical Parameters from Ekruk Tank, Solapur

M. R. Guarv1 and K. R. Rao2

1Department of Zoology C.B. Khedgi’s College, Akkalkot 2Department of Zoology Walchand College of Arts & Science, Solapur

Abstract: Ekruk tank (Lat 170 44.40.02’,Long 075054.51.79’E elev 461m) is a freshwater lake near Solapur city (away from 10 km towards Tuljapur road). This lake has the capacity (14.675.46 cu.mi).The main purpose of this lake is for water supply and irrigation. It is a breeding site for ma-jority of migratory avian population. This artificial lake in fact constructed during British Ira and was the main source of drinking water for some older parts of the Solapur city and even for Ekruk village. Zooplanktons are the main source of food for the aquatic inhabitants for fishes and other fauna. Because of various human activities nearby Ekruk tank like Dhobi ghat, vehicle washing, cattle washing, fishing etc.has adverse impact on the diversity of zooplanktons from Ekruk tank. It was also noticed that physic- chemical parameters has greater influence on diversity and production of zooplanktons in an aquatic body. The present study is aimed to understand human interventions and it’s relation on the diversity of zooplanktons with special reference to changes in physico- chemical parameters.Keywords: Ekruk Tank, Zooplanktons and Physico- chemical parameters

Diversity of Freshwater Bryozoa in IndiaSatish Mokashe1, Nirmiti Nimbalkar2 and Harkal Ananta3

1&2Department of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad3Department of Zoology, New Arts, Commerce and Science College, Ahmednagar

Abstract: The article reviews the diversity and distribution records of freshwater Bryozoa in India. The two classes namely Gymnolaemata and Phylactolaemata are represented by 28 species. The Gymnolaemata is represented by four species and phylactolaemata is represented by 24 species. The papers also give you an idea about the distribution records of all 28 species in 18 states and 1 union territory of the India and reveal vacant the areas which are have to be explored for understand the real picture of diversity and distribution of these animals in India. Keywords: Bryozoa, Diversity

ISBN: 978-93-5382-353-5


Page| 18

Some of the Aromatic Pants and Their UsesMadhu Laxmi Sharma

Govt. K.R.G. P.G. Autonomous College, Gwalior

Abstract: Plants have been used for therapeutic, religious, cosmetic, nutritional, and beautification purposes since ancient times and humanity of all civilizations and culture are familiar with their usage. Plants are an important source of medicines and play an important key role in human health. Among a large group of plants, many of them are actively used as a source to treat or to prevent many ailments. Aromatic plants are a particular group of plants that produce fragrance and they are used for their aroma and flavor. Aromatic compounds are present in different parts of plants i.e. in root, wood, bark, foliage, flower, fruit, seed etc. Some of them are used for medicinal purposes. Aromatic plants are a good source to develop a new source in alternative therapy and to treat body and to keep mind fresh. Large number of aromatic plant parts and their extracts are used for me-dicinal purposes, in aroma therapy as well as in various systems of medicines. Humans have used them throughout historical time either to cure or to keep them fresh or to prevent from different ailments. Aromatic substances are also used in making perfumes, in cooking, in food, pharmacy, industries etc. Apiaceae, Lamiaceae, Lauraceae and Myrtaceae are some of the important families with reference to aromatic purpose. The accumulated massive knowledge, information, must be shared in the society and go down to generation to generation.Keywords: Aroma, Medicinal, Prevent, Pharmacy and Industries

Molecular Taxonomy in Diversity of Spider of Beed DistrictNagawanshi M. N.

P.G. Dept. of Zoology, Deogiri College, Aurangabad

Abstract: Biodiversity of spider fauna of Beed district of Maharashtra was studied using DNA barcoding at molecular. The investigation was carried out for 2 year from June 2013 to May 2015. Sampling was done seasonally. Spiders were collected in from different spots by various methods. Spider population in different seasons exhibited variation in species abundance and composition. Ten spider species belonging to five spider families were recorded.Keywords: Molecular taxonomy, spider, Beed

ISBN: 978-93-5382-353-5


Page| 19

Biodiversity of Zooplanktons from Mahasangavi ReservoirR. M. Dhere1, J. M. Gaikwad2 and R. G. Pradhan3

1Department of Zoology, Swa. Sawarkar Mahavidyalaya, Beed, Maharashtra 2Department of Fisheries,Shri. Shivaji College, Parbhani, Maharashtra

3Department of Zoology, Lalbahadur Shastri College Partur, Jalna, Maharashtra

Abstract: The present investigation was carried out to study the zooplankton at the Mahasanga-viResrvoir, Taluka Patoda District Beed. Variation and the abundance of zooplankton during the study period from January 2014 to December 2014 were described. Higher population density of zooplankton in rainy season is observed and the results are discussed. The members of the zoo-plankton community are important for their major role in the tropics dynamics and in energy trans-fer in the aquatic ecosystem. They provide food for fishes in the freshwater bodies and play a major role in the fish production (Kodarkar M.S 1999) The zooplankton samples were collected from from January 2014 to December 2014 during early morning hours using a plankton net with mesh of 100 m (IAAB).The zooplankton samples were preserved in 5% formalin. The different species of Rotifers, Cladocerans and Copepods were isolated and were studied under compound microscope for their taxonomic identification using standard taxonomic publications Kodarkar (1998), Dhana-pathi (2000) and Sharma and Sharma (2008).

In the present investigation zooplanktons are collected from the Mahasangavi Reservoir. The zoo-planktons are the main feed of larval stages of fishes. The fish seed stocking and production from the water bodies is depend on the availability of the natural food i.e. Zooplanktons it enhances the survival of early stages of fish if it is available in water bodies. Zooplankton samples are collected with the zooplankton net. Taxa are identified and counted under a Microscope Labomed CXL. In marathwada region the fry and fingerling of Indian major carps and exotic carps are stocked during July to September everyyear at the time of stoking proper timing is also an important for optimum growth of the fry. Fryand Fingerling fishes will be consuming zooplankton faster than it can be produced in water. Butno anyone check the availability of the qualitative and quantitative zooplanktons at the time of stocking fry and fingerling, so there is a need of establishment of field laboratory at the reservoir site for the analysis of zooplanktons. It helps in the survival of larval stages of fishes and enhances the fish production because in reservoir waters, the supplementary food is not given to the fishes it will grow on available of natural food i.e. zooplankton in reservoir waters. There is a need of scientifically and technological culture practices in reservoir waters for growing of freshwater fishes and increase in fish production.Keywords: Mahasangavi, Reservoir, Zooplanktons, Biodiversity

ISBN: 978-93-5382-353-5


Page| 20

New Approaches for Conservation of Exotic Plant SpeciesDeepa N. Muske1, Manorama B. Motegaonkar2

1Dr. PDKV, Akola 2Azad College Ausa

Abstract: The contribution of native plants to the ecological balance and to the preservation of the environment has been recognized, development of methods for tissue culture of native plants for propagation and conservation is lagging behind. The major threat to rapid loss and extinction of genetic diversity due to habitat destruction, pollution, climate change, invasion of exotic species, human population pressure, ever increasing agricultural pressure and practices, life style change etc. are well-known. There are different methods for conservation are available among them Tissue culture technology is utilized for the conservation and mass propagation of selected native plants that cannot be propagated on a large-scale by means of seeds and cuttings. The global concern of biodiversity conservation initiated either by in situ or ex situ methods. In situ methods protect both plants and their natural habitat. On the other hand, ex situ methods involves preservation and maintenance of plant species or plant parts (such as seeds, cuttings, rhizomes, tubers etc.) outside their natural habitat for the purpose of developing seed banks or more preciously gene banks fol-lowing classical / advanced methods of plant propagation. Classical methods of plant propagations have certain limitations in terms of rapid production of plants or plant propagules and their long term conservation. So, the biotechnological methods such as plant tissue culture, plant cell culture, anther culture, embryo culture etc. are quite applicable and useful techniques for ex situ conserva-tion. On the other hand, the production of superior quality seeds has enhanced by the application of plant biotechnology. So, plant biotechnology offers new means of improving biodiversity conser-vation rather than threatening biodiversity in various ways. In vitro plant propagation is a helpful technique in the conservation of genetic diversity of all types of plants (including rare, threatened and endangered plants) in a rapid and reliable way by maintaining the same clone or stock of plant material. It is just an efficient alternative method of plant propagation technique. It goes well to the natural process of plant conservation to restore the declined ecosystem with tremendous applica-bility.Keywords: conservation, tissue culture, cryopreservation, plant tissue, exotic

ISBN: 978-93-5382-353-5


Page| 21

Zooplankton Diversity Status from Kurnur Dam in Akkalkot in Solapur District

Anirudhh D. Babare1, M. G. Babare1 and M. B. Tat2

1Dept. of Zoology, A.C.S. College, Naldurg, Osmanabad2Dept. of Zoology, A.C.S. College, Kille-Dharur, Beed

Abstract: The Zooplankton’s are the richest source of the Aquatic animals for fishes. In standing water bodies many Zooplankton’s act as a food for various fishes. This dam located near Kurnur village Tal- Akkalkot, Dist-Solapur. During the present study period the water samples collected from the Kurnur dam with the interval of one month during the period of one year (June 2014 to May 2015) from the selected spots of Kurnur dam. A result shows that the Kurnur Dam contains the Zooplankton’s represented by 16 species. There are 6-species belonging to rotifer,4 species belonging to class cladocera, 4-species belonging to copepod and 2-species belonging to ostracoda.Zooplanktons are the producers in the aquatic environments. The Kurnur Dam is located about 13 Km from Akkalkot city, at latitude 17°37’ 0” N and longitude 76°13’ 2” E. The details diversity of Zooplankton’s of Kurnur Dam discussed in this text.This work was carried out for one year (June2014 to May2015).Keywords: Diversity-zooplanktons- Kurnur Dam, Solapur

A Study on Icthyo-faunal Diversity of Sukhana Dam, Garkhrda, Dist. Aurangbad, MaharastraA. S. Munde1 and P. R. More2

1Department of Zooology, Sambhajirao kendre Mahavidyalaya, Jalkot Latur2Department of Zooology, Kai. Rasika Mahavidyalaya, Deoni, Latur

Abstract: The present study deals with fish biodiversity undertaken during period July2011 to June 2012 to survey and commercially important fishes in the Sukhana dam.The Fresh water body of Sukhana dam used for irrigation purposes at Garkheda in Aurangabad district. The present study deals with the variety and abundance of fresh water fishes in Sukhana dam of Garkheda in Aurang-abad district (M.S.) India. The results of present study reveal the occurrence of fish biodiversity belong to 4 orders 7 families and 16 species. The members of Order Cypriniformes were dominated by 9 species followed by Perciformes 4 species, Siluriformes 2 species and Synbranciformes with one species.Keywords: Icthyofaunal diversity, Sukhna dam Garkheda, Economic value

ISBN: 978-93-5382-353-5


Page| 22

Planktonic Biodiversity and Its Important Role in Fish Production on Majalgaon Dam Reservoir, Maharashtra

Sitaram B. Ingole1 and PrashantSanghai2

1Shri Siddheshwar Mahavidyalaya Majalgaon, Beed2ShriShivaji College, Kannad Dist. Aurangabad

Abstract: Majalgaon Dam was constructed on the River Sindphana which is a tributary of River Godavari, in Beed District (Maharashtra, India) in 1987.The River Sindphana has been under con-stant threat of pollution by sewage and industrial wastes, disposal of dead bodies, deforestration, excessive useof fertilizers and pesticides, bathing and water development programmes. The dam has a catchment area is 3840 sq. km. It is of great Importance for the region because its water is used for human and cattle consumption, power generation, fish production and irrigation. A total of 33 species of phytoplanktons, 29 species of zooplanktons and 11 species of fishes were identified.The importance of plankton in fisheries is well established. It has been clearly demonstrated that the zooplankton constitute the only food for the fish fry and the adult fish not only eat them, but also select them as a delectable item.Thus zooplankton have a direct bearing in the fish industry. In India, several studies were conducted in reservoirs elucidating the characteristics of zooplankton.The zooplankton peak was found during summer followed by winter and rainy season.Microfauna (zooplankton) was observed about four groups as Rotifera observed about eleventh species, Co-pepoda observed about nine species, Cladocera observed seven species and Ostracoda observed about two species.The macrofauna or fish fauna were observed at the Majalgaon Dam reservoir. There are cultures of fish with quick growing varieties of fishes including Indian Major Carps, exotic species have been popular in recent time. There is abundance of the species such as Labeorohita, Cirrhinamrigal, Catlacatla, Cyprinuscarpio, Silver carp, Wallagoatta, Mystacenbelusarmatus, Notoptemus chital, Puntuesticto, Channastaitus, Mystusseenghala, Mystuscavaassius, Eutroplussuratensis, Beloncon-cila, Chela, Tilapia Mossambica, Rohteealfrediana, Gobiusgiuris,etc.Fish is economically a very important group of animals beside being used as food. Fish liver is an important source of oil con-taining Vitamins A and D, several minerals and protein.Keyword: Phytoplanktons, zooplankton, pollution, fish production

ISBN: 978-93-5382-353-5


Page| 23

Biodiversity of Zooplanktons of Dhamana Reservoir, Shelud, Tehsil Bhokardan, Dist. Jalna

Pradip J. MisalDept. of Zoology, Siddharth Arts, Commerce and Science College Jafrabad, Jalna

Abstract: The present investigation was carried out to study the zooplankton diversity at Dhama-na Dam, Shelud Tehsil Bhokardan Dist. Jalna. The zooplankton biodiversity of reservoir water is represented by five different groups like Protozoa, rotifera, cladocera, copepoda, ostracoda, with 18 different species. This water body is utilized by local residents for cloth washing and open defecation on the reservoir banks is responsible for fast deterioration of the lake ecosystem due to these anthropogenic activities. Siltation is also playing a crucial role in clogging this beau-tiful ecosystem to a large extent along with death and decay of aquatic macrophytes. If the present trend of enrichment continues for some years this water body will soon be degraded fully and con-vert into a dry land in near future.Keywords: Biodiversity, Zooplankton, Dhamana dam

ISBN: 978-93-5382-353-5


Page| 24

ISBN: 978-93-5382-353-5


Page| 25

GROUP- BEnvironment

ISBN: 978-93-5382-353-5


Page| 26

ISBN: 978-93-5382-353-5


Page| 27

An Analysis of Noise Pollution in Osmanabad City, Maharashtra Shoeb Peerzade1, S. V. Thakur1, R. L. Ware1, Sayed Abed2

1Department of Chemistry, Milliya Arts Science & Management Science College, Beed, Maharashtra

2Department of Chemistry, Government College Of Arts & Science Aurangabad Maharashtra

Abstract: Noise pollution, also known as pollution of sound environmental noise or sound pollution, is the propagation of noise with harmful impact on the activity of human or animal life. Poor urban planning may give rise to noise pollution side by side industrial & residential buildings can result in noise pollution in the residential areas. Some of the main sources of noise in residential area include loud music, transportation noise, and lawn care maintenance nearby construction. Noise Pollution can be defined as unwanted or excessive sound that can have deleterious effect on human health and environmental quality. World Health organization stated that “Noise must be recognized as major threat to human wellbeing” According to the USEPA there are direct links between noise & health. Also noise pollution adversely affects the liver of millions of people. Noise pollution can damage physiological & psychological illness sleep disruption, hearing loss are the problems related to noise pollution.In the present investigation study of traffic noise in Osmanabad city has been done in the busy area of the city which has high traffic flow in peak hours. The result obtained was compared with standard values for noise level under the Air Act 1981 given by the central pollution control Board (CPCB) of India. Result obtained in the study shows that whole city is affected heavily by noise pollution and in almost 80% of the area prevailing noise level is more than the ambient noise level. It has been found that in many areas the noise level prevailing averages around 85 dB at 80% of the busy points of the city. Most of the noise is generated only due to horns of vehicles like rickshaw, buses, & trucks etc.Osmanabad city is being a small & congested creates chronic issues related to noise pollution. The city is developing more & more vehicle are being used regularly which adds thrust on noise level in the city which is in turn will create many health issues. Since traffic noise is increasing more in this city day by day. So this has to be analysed & controlled so that no health hazards pose.Keywords: Noise Pollution, Noise, Monitoring Noise Data, Osmanabad City

ISBN: 978-93-5382-353-5


Page| 28

Effect of Integrated Nutrient Management on Soil Properties of Indian Mustard (Brassica juncea L.) Cv. Sangam

Vinod Kumar Boddepalli and Arun Alfred DavidDepartment of Soil Science and Agricultural Chemistry [Naini Agricultural Institute]

Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj, U. P.

Abstract: A field study was conducted at the Soil Science Research Farm, Sam Higginbottom University of Agriculture, Technology and Sciences, Prayagraj during Rabi season 2018. The soil of experimental area falls in order Inceptisol and soil texture was sandy loam. The result showed bulk density, particle density and pore space % decreased with the increased application of fertilizers. The pH and EC of the post-harvest soil decreased with the increased levels of fertilizers where the highest values were obtained in control plots. The organic carbon 0.76 % found to be increased with fertilizer levels [100% NP&K+100% vermicompost & poultry manures] in T8. The N, P & K content in the soil increased with levels of fertilizers in treatment T8. The lowest values related N P & K parameters were obtained in control plot treatment. Benefit cost ratio (B:C) 1:2.5 was highest in T8 was more profitable 55612.00 ha-1 than any other treatments and recommendation.Keywords: Soil properties, nutrients, poultry manures, vermicompost, mustard, cost benefit ratio

A Study of Environmental Impact of Pesticides on Water of Ambadi Dam, Kannad, Aurangabad

Jadhav T. J.Department of Zoology, Shivaji Arts Comm. and Science College, Kannad, Aurangabad

Abstract: Pesticides are the chemical products used for plant protection. They include Insecticides, Fungicides, and Herbicides. Over 98% of sprayed insecticides and 95% of herbicides reach a destination other than their target species, including non-target species, and water. Indiscriminate use of organic pesticides kill the natural enemies of pest.Organic pesticides can contaminate unattended land and water when they are sprayed aerially or allowed to run off fields soils from production site and storage tanks or are inappropriately discarded. The amount of pesticides that migrate from the intended use area is influenced by the particular chemical properties. Soil binding, water vapour pressure and water solubility. Factors in the soil such, as its texture ability to retain water and amount of organic matter contained in it. Keywords: Pesticide, water and fish.

ISBN: 978-93-5382-353-5


Page| 29

Current Status and Future Challenges of Green ChemistryIshwar Baburao Ghorade1 and Krti Sadhurao Niralwad2

1Department of Environmental Science, Kohinoor Arts, Commerce & Science College, Khultabad, Aurangabad

2Department of Chemistry, Nutan Mahavidhyalaya, Selu, Parbhani

Abstract: Green chemistry emerged from a variety of existing ideas and research efforts (such as atom economy and catalysis) in the period leading up to the 1990s, in the context of increasing attention to problems of chemical pollution and resource depletion. The development of green chemistry in Europe and the United States was linked to a shift in environmental problem-solving strategies: a movement from command and control regulation and mandated reduction of industrial emissions at the “end of the pipe,” toward the active prevention of pollution through the innovative design of production technologies themselves. The set of concepts now recognized as green chemistry coalesced in the mid- to late-1990s, along with broader adoption of the term (which prevailed over competing terms such as “clean” and “sustainable” chemistry). Green Chemistry is a relatively new emerging field that strives to work at the molecular level to achieve sustainability. The field has received widespread interest in the past decade due to its ability to harness chemical innovation to meet environmental and economic goals simultaneously. Green Chemistry is ensuring that all of that creative ability that is the long tradition of the field of chemistry is practiced in a way that builds in impact on people and the planet as a design criterion.Keywords: Environment, Green Chemistry, Pollution and Sustainable

Study of Different Water Borne Diseases Found in Different Slum Areas of Nashik District, Maharashtra

Rohan Kumar Yadav, B. S. Yadav, D. B. GoswamiK. J. Somaiya College Kopergaon, Ahmednagar, Maharashtra

Abstract: In the present research,survey of different slum areas has been done to check the status of water borne diseases in slum area of nashikcity.Different types of water samples were collected from different slum areas viz. Malharkhani, Phulenagar, Someshwar, Sadhuvasvani, Gadge Maharaj area, Old civil hospital, Bhadrakali, Mangalwadi.It has been observed that due to poor sanitation facility water borne diseases were occurred more.

ISBN: 978-93-5382-353-5


Page| 30

Microbial Profiling of Street Food (Panipuri) at Different Locations of Parbhani City, India

Sunita R. MukkawarB. Raghunath College, Parbhani, Maharshtra

Abstract: Food poisoning and other food borne diseases occur through poor hygiene practices, especially in areas where food served by vendors. In most cities around the world, selling of whole meals on the streets is an important means of generating income. The emergence of informal food business can cause health problems if the foods are not prepared and handled under hygienic conditions.The present study was focused to investigate the microbial quality of street vended food (panipuri) which is delicacy of most people in Parbhani city. The aim of the study was to judge potability of street food materials (panipuri) consumed by 70% of people in Parbhani city.Total of 27 samples from three popular sites of Parbhani city were collected. The dominant bacterial species recorded were Escherichia coli and Bacillus species followed by Staphylococcus aureusand Salmonellatyphi. The highest bacterial load was observed in samples of site I. i.e. (29×102 c.f.u./ml) followed by site II i.e. 7×103c.f.u./ml in sample.Comparative analysis of Puri, Khattapani and Masala stuff revealed that Masalastuff showed highest bacterial load specifically with respect to E. coli indicating high contamination and high health risk. Keywords: Street Vended Food, Hygine, Health risk, Salmonella typhi, Escherichia coli

Allergenic Fungal Spore Monitoring in Some Intramural Sites of Aurangabad City

S. K. Rajurkar, A. S. Taware and S. N. GaikwadDepartment of Botany, Deogiri College Aurangabad

Abstract: Intramural environment provides congenial atmosphere for allergens as well as microorganisms. Contribution of Allergens and Microorganisms are more in intramural environment than outside due to low temperature and moist humid climate. For this study experiment was carried out from 1st Jan 2015 to 31 Dec 2015 at six places of Aurangabad city. Altogether 26 fungal types were recorded out of which 15 are Allergenic, 08 are deteriorating while remaining are saprophytic fungi. Alternaria alterata, A.tennis, Aspergillus fumigatus, A.flavus, A.niger, Nigrospora sp, Penicillium sps are found to be dominant allergens at all sites. Keywords: Allergens, Aspergillus, Deteriorating fungi

ISBN: 978-93-5382-353-5


Page| 31

Air Quality Assessment of Aurangabad City, MaharashtraTiwari R. B.1, Jabde P.V.1, Motghare V. M. 2, Kollur S. C. 2 and Kadam J. A.2

1S.B.E.S. College of Science, Aurangabad2Maharashtra Pollution Control Board, Mumbai

Abstract: Now a day’s urban areas are not suitable for people in who preferred going on jogging or morning walk. Many researchers are of the opinion that walking in the morning especially in urban areas dominated by industrial activity is not only unsafe, it is harmful. It is harmful especially for the patients of asthma and other bronchial or respiratory disorders. As the night movers towards morning these pollutants tend to settle down and move horizontally with the blowing morning air in which we walk in the morning.This paper assesses the ambient air quality status in Aurangabad city. Aurangabad city is a tourism hub, surrounded with many historical monuments including the Ajanta & Ellora caves recognized as world heritage sites, as well as Bibi-Ka-Maqbara & Panchakki and is the administrative headquarter of the Marathawada region. One of the major sources of urban air quality is the road transport sector. Besides, domestic, commercial and industrial activities also contribute to urban air quality. It is reported that over 70 – 80 % of air pollution in mega cities in developing nations is attributed to vehicular emissions caused by a large number of older vehicles coupled with poor vehicles maintenance, inadequate road infrastructure and low fuel quality. The sampling of the pollutants was done by using Respirable Dust Sampler (RDS). The investigation into seasonal ambient air quality data for Aurangabad has shown that the air quality moderate in winter months like Nov., Dec., and Jan. The Pre-monsoon months resulting in high PM and SPM concentrations but lesser than those in winter. The air quality is better in monsoon and post monsoon.The observation concluded that the concentration of air pollutants, SO2 & NO2 are comparatively with in limit as per CPCB standards. The results revel that PM10 & SPM concentration are excess as per CPCB standards. The high concentration of PM at the residential and commercial area may be attributed due to resuspension of road dust, soil dust, and automobile traffic. High dust levels due to local construction, unpaved roads etc. Overall results indicate that RSPM & SPM pollutants are one of the major causes for deterioration of ambient air quality of the city. Keywords: Aurangabad, Air quality, PM10, RDS

ISBN: 978-93-5382-353-5


Page| 32

To Study Different Waste Water Samples and Recycling-Reuse by Developing Evaporating Cone Arrangement and Physical Filtration

MethodGaurav Ruprao Rahate

Smt. Sindhutai Jadhao Science College Mehkar, Buldana

Abstract: Water crisis is one of the most increasing issues before every developing country. The wrong water management techniques and human habits is the major factor that affects the decrementation of water resource and day by day making the eco-system more and weaker. To overcome this future disaster we need some solution that rebuilds the ecosystem by using various RECYCLING methods. The main focus of this article is to collect the water samples from different places of waste water sources. The samples are studied by using various laboratory parameters such as Physical, Inorganic OR Chemical, Toxic Metals, Organic Nutrient & Demand, Biological elements Bacteriological etc. whatever possible. On the basis of the parameters the various physical filtration techniques are studied and cheaper techniques are describe that are used to make the water useful for either drinking or any other domestic use without harming other resources. Another part of the article is based on the development of the cheap technique of water recycling based on the phenomenon of EVAPORATION and CONDENSATION. The physical state of matter exist the VAPOUR state which can be used to extract the water droplets collected from the EVAPORATING CONE by the use of reversible processes in normal temperature. The detail study of sample, recycling techniques and the Evaporating cone and the result gain is given in the paper and an attempt is made to illustrate some things that are important to overcome the water crisis and conservation of natural resource. Keywords: Evaporation, condensation, cone, recycling, conservation of water

Nemerows Pollution Index and % Na in the Ground and Surface Water of Ranjangaon & Jogeshwari Area

Mohammad Mohsin and Megha RaiDr. Rafiq Zakaria College for Women, Navkhanda, Aurangabad

Abstract: Considering NPI as pollution index station S3 and S5 are found to be pollution free. The most commonly used index for the determination of quality of water is (WQI)2. From which it is clear that the water is unfit for human consumption. We observed that, the water quality from various stations is not used for human consumption by local people.Keywords: Water Quality index, pollution, Industrialization, Fertilizers, domestic waste

ISBN: 978-93-5382-353-5


Page| 33

Study of Fungal Spores in Library Environment and Their Effect on Human Health

S. K. Rajurkar1, M. N. Nagwashi2 and S. N. Salve1 1Department of Botany, 2Department of Zoology, Deogiri College, Aurangabad

Abstract: Present study deals with the release of Allergenic fungal spores in library environment and their effect on human health. The studies were carried out during 1st Jan 2016 to 31st Dec 2016. Fungal spores collected with the help of “Tilak air Sampler” as well as exposing “Potato-dextrose agar” containing Petri plates at fortnightly intervals. DNA Barcoding method is used for identification of taxon up to species level. Aeroallergens contributed (81.84%) to the total airospora. Allergenic fungal spores reported are Chetomium (21.88%), Alternaria (20.71%), Aspergillus (12.50%), Curvularia (9.82%), Fusarium (7.45%) and Cladosporium (2.07%). These fungal spores were correlated with Metrological conditions. The higher incidence of aeroallergens effects on human health. Keywords: Airospora, Aeroallergens, Tilak air Sampler and Metrological conditions

Study of Density and Ultrasonic Velocity of Phenobarbital Sodium at Different Temperatures

S. B. Ingole, C. D. Thakur, A. G. ShankarwarDepartment of Chemistry, S.B.E.S. College of Science, Aurangabad

Abstract: The study of Density and Ultrasonic Velocity of Phenobarbital Sodium in aqueous solution provides information useful to elucidate ion–ion, ion–solvent, and solvent–solvent interactions. Apparent molar volumes (Φv), and Acoustic Impedance in aqueous system have been determined from density (ρ) and ultrasonic velocity measurements at four different temperatures using the ANTON PAAR densitometer. The apparent molar volumes were calculated from the density data. In addition adiabatic compressibility is also determined. The concentration dependence of the apparent and partial molar volumes can be used to study ion–ion interactions, whereas the partial molar volumes at infinite dilution provide information on ion–solvent interactions.

Keywords: Phenobarbital sodium, density adiabatic compressibility, acoustic impedance

ISBN: 978-93-5382-353-5


Page| 34

Analysis of Physico-Chemical Water Quality to Assess Environmental Degradation of Nagzari Dam from Latur District

Kolpuke M. N.1 and Pathan A. V.2

1Department of Zoology, Maharashtra Mahavidyalaya, Nilanga, Maharashtra2Department of Zoology and Fishery science, Azad College, Ausa, Maharashtra

Abstract: The quality of surface water has progressively worse in many countries in the past few decades. As a result of the growing population, urbanization, agriculture, and increasing industrialization, the inland water bodies are confronted with the increasing water demand, as facing with extensive anthropogenic emissions of nutrients and sediments, predominantly the lakes and reservoirs. To resolve this problem, it is necessary to carry out water quality assessment, planning, and management, in which water quality monitoring plays an important role. This study aimed at assessing the water quality of Nagzari Dam from Latur district (MS) India.Nagzari Dam used for irrigation, livestock watering and fish production. This study carries using some selected physico-chemical parameters. The result of water samples shows high pH indicates the basic nature of water samples, sulphate in the dam water was high, the phosphate content of reservoir water were found high which lead to unpleasant taste and odor. The obtained values of each parameter were compared with the standard values set by the World Health Organization (WHO). The values of each parameter were found to be within the beyond safe limits set by the WHO. Overall, the water from all the locations was found to be not safe as drinking water. However, it is also important to investigate other potential water contaminations such as chemicals and microbial and radiological materials for a longer period of time, including human body fluids, in order to assess the overall water quality of Nagzari Dam. Keywords: Water Samples, Environmental Degradation, Nagzari Dam.

Extraction and Separation Studies of Platinum(IV) with DMABIMTT as an Extractant

Uzma Parveen K. ShaikhDr. Rafiq Zakaria College for Woman (Navkhanda), Auranagabad M.S. India

Abstract: An effective method is proposed for the extraction of Platinum (IV) with 4-(4-dimethylaminobenzylideneimino)-5-methyl-4H-1, 2, 4-triazole-3-thiol (DMABIMTT) in chloroform as an extractant from hydrochloric acid media. In this method Platinum is extracted by using solvent extraction technique with DMABIMTT as an extractant which is stripped and determined spectrophotometrically. The effect of diverse ions on the quantitative extraction of platinum has also been studied. The method is applicable for the separation of platinum from binary mixtures, alloys, synthetic mixtures and commercially available samples.Keywords: Solvent extraction, Platinum metal, extractant (DMABIMTT), hydrochloric acid media

ISBN: 978-93-5382-353-5


Page| 35

Study of Apparent Molar Volume and Adiabatic Compressibility of Cetrizine Hydrochloride at Different Temperatures

G. S. Sanap, S. B. Ingole and A. G. ShankarwarDepartment of Chemistry, S.B.E.S. College of Science, Aurangabad

Abstract: The study of Apparent molar volume and Adiabatic Compressibility of Cetrizine Hydrochloride in aqueous solution provides information useful to elucidate ion–ion, ion–solvent, and solvent–solvent interactions. Apparent molar volumes (Φv), and Acoustic Impedance in aqueous system have been determined from density (ρ) and ultrasonic velocity measurements at four different temperatures using the ANTON PAAR densitometer. The apparent molar volumes were calculated from the density data. In addition adiabatic compressibility is also determined. The concentration dependence of the apparent and partial molar volumes can be used to study ion–ion interactions, whereas the partial molar volumes at infinite dilution provide information on ion–solvent interactions.Keywords: Cetrizine HCl, density adiabatic compressibility, acoustic impedance

Degradation of Environment by Volatile Organic Compounds: Causes and Solutions

Arti D. Rushi1, Kunal P. Datta2, Prasanta S. Ghosh3 and Mahendra D. Shirsat4

1Basic Sciences and Humanities Department, Maharashtra Institute of Technology, Aurangabad2Deen Dayal Upadhyay Kaushal Kendra, Dr. Babasaheb Ambedkar Marathwada University,

Aurangabad3Department of Basic Sciences and Humanities, International Centre of Excellence in

Engineering and Management, Aurangabad4RUSA Centre for Advanced Sensor Technology, Dr. Babasaheb Ambedkar Marathwada

University, Aurangabad

Abstract: Volatile organic compounds (VOCs) one of the popular industrial solvent used in the fabrication of various products. Due to volatile nature of the compounds, their concentration in the air atmosphere is quite challenging. All living species when become part of such environment seems to suffer adverse effects of VOCs exposure. Putting limits on the usage of VOCs is quite critical task. Precaution could be taken by monitoring the VOCs concentration in the atmosphere. To deal with this challenge, in the resent investigation a solution is provided in the form of smart sensor device. Sensors were fabricated with single walled carbon nanotubes. After fabrication structural, electrical characterizations were carried out. Concentration of VOCs was detected in the chemiresistive modality of sensing. It has been well observed that fabricated sensor systems are highly applicable to monitor concentration of VOCs in the environment.

ISBN: 978-93-5382-353-5


Page| 36

3D-QSAR and Docking Studies of 2-styrylquinolines as Epidermal Growth Factor Receptor Inhibitors for Anticancer Activity

Kshipra S. Karnik1, Aniket P. Sarkate1, Deepak K. Lokwani2, Pravin S. Wakte1

1Dept. of Chemical Technology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad2R. C. Patel Institute of Pharmaceutical Education & Research, Shirpur

Abstract: A continuous and undefined malignant growth is seen in cancer that makes it an extremely heterogeneous complex disease. Over expression of epidermal growth factor receptor (EGFR) protein tyrosine kinases in most of human cancers helps in the development of novel anticancer molecules. Here, the predictive 3D-QSAR models using different molecular modeling techniques were carried out using a set of 10 novel 2-styrylquinoline derivatives acting as EGFR inhibitors. Five-point common pharmacophore hypotheses were selected for the alignment of all the compounds. The best pharmacophore hypothesis AHHRR.10 was selected and a 3D-QSAR model was generated by applying partial least-squares algorithm. The activity of compounds from literature was compared with the actual activity by performing external validation of the developed CPHs and 3D-QSAR models. The binding affinity of compounds for anticancer activity was checked by carrying out the docking studies for all compounds on EGFR enzyme. The designing of novel potent compounds becomes easy with the help of the molecular modeling studies by refining the pharmacophore.Keywords: 3D-QSAR; EGFR; Anticancer activity; Quinoline; Docking

Ecological Health Status of Sip River, a Tributary of River Narmada Using QBR Index

Kuldeep Lakhera1, Parul Gurjar1 and Vipin Vyas2

1Department of Environment Science and Limnology, Barkatullah University Bhopal2Department of Bioscience, Barkatullah University, Bhopal

Abstract: Riparian zone is an interface between the terrestrial and aquatic ecosystem which provides a variety of resources to the organisms. The present study was carried out on Sip River which is a tributary of River Narmada. During the study, QBR (Qualitat del Bosc de Ribera) index was used for the assessment of quality of riparian forest. It was observed that out of seven sampling stations, two stations were found in bad quality and extreme degradation on riparian habitat, three stations were found in Fair quality and strongly altered riparian habitat where as two Stations were found in Good quality and some disturbances were observed in these areas. The current status of riparian zone was due to human activities like construction, sand mining, soil mining, deforestation and expansion of crop land for agricultural activities. Livelihood dependence is the major cause of present condition of riparian zone. Keywords: Riparian zone, Sip River, Narmada River, QBR index

ISBN: 978-93-5382-353-5


Page| 37

Water Quality Studies of Jangi Lake in Ambad, MaharashtraD. D. Bhutekar1, A.B.Daspute2, R.F.Pathre1 and S. B. Aher3

1MSS’s Arts, Science and Commerce College, Ambad, Jalna, Maharashtra2S.B.E.S College of Science, Maharashtra

3Indian Institute of Soil Science, Bhopal, Madhya Pradesh

Abstract: Jangi Lake was constructed by Devi Ahilyabai Holkar to fulfill the need of drinking water demand for Ambad town, Dist- Jalna, Maharashtra (India). The physico-chemical and ecological parameters of water of Jangi Lake was studied to assess the status of water quality. The water samples were collected monthly during 2017-18 from three different sampling points and analyzed for pH, EC, DO, BOD, COD, phosphates, nitrates etc. Present study reflects that, the urban development in the town led adverse changes in the physico-chemical and ecological characteristics of Jangi Lake water. The urban Sewage runoff, washing, bathing and dumping of solid waste, Ganesh idol immersion, and other activity deteriorated the water quality of Jangi Lake and it is getting enriched with plant nutrients and other pollutants, becoming more and more infested with macrophytes , getting slowly shallower and shallower and shrinking gradually. In conclusion, the water is polluted and unfit for drinking, bathing purpose without any treatment. The eutrophic condition affected the aesthetic value of Jangi Lake. The suggested strategies for conservation of Jangi Lake on the basis of conducted study are prevention of pollution, diverting of sewage waste water, treatment of waste water, lake cleaning by de-silting, de-weeding, bioremediation, public awareness and public participation.Keywords: Jangi Lake, idol, Lake Conservation, Water quality, Physico-chemical analysis

Phytoremediation Best Approach for Management of PollutionManorama B. Motegaonkar

Department of Chemistry, Azad College Ausa, Maharashtra

Abstract: Anthropogenic activities are resulting in an increase on the use and extraction of heavy metals. Heavy metals cannot be degraded and hence accumulate in the environment having the potential to contaminate the food chain. This pollution threatens soil quality, plant survival and human health. The remediation of heavy metals deserves attention, but it is impaired by the cost of these processes. Phytoremediation is sound environmental technologies which could be at the forefront to mitigate soil pollution. This provides an overview of the current state of knowledge phytoremediation application to remediate heavy metal contaminated soils, discussing the advantages and disadvantages of both individual approaches and use of different plants for the same. Research to date has attempted only in a limited number of occasions to combine both techniques, however we discuss the potential advantages of combining both remediation techniques and the potential mechanisms involved in the interaction between phyto remediators. We identified specific research needs to ensure a sustainable use of phytoremediation as remediation tools.

ISBN: 978-93-5382-353-5


Page| 38

Assessment of Demand Parameters of Nathsagar Reservoir at Paithan, Maharashtra

Rahul Mahamuni and Kshama KhobragadeDepartment of Environmental Science, S.B.E.S. College of Science, Aurangabad

Abstract: Jayakwadi dam, a lifeline of Marathwada; is one of the largest irrigation projects in the Indian state of Maharashtra. To provide water for irrigation and drinking purpose was amajor aim while constructing such a largest earthen dam in the drought-prone Marathwada Region. Water level in Jayakwadi has hit dead stock in summer 2019, with just 1.5% live storage left. Its dead stock is about 26 TMC. However, a major chunk of it has been filled with silt, reducing actual availability of water.The study was carried out from June 2018 to May 2019. The water samples were collected monthly for a period of one year from the three sampling stations from upstream area. Parameters like BOD, COD and DOare also known as demand parameters which were showed variations throughout the study period along with other physico-chemical parameters.These three parameters have an intact relationship to indicate the pollution status. Demand parameters are an important water quality parameter because it provides an index to assess the effect of discharged wastewater on the receiving aquatic environment.The values of BOD and COD were quite high whereas DO found remarkably at lower range i.e. above permissible limits. In brief, the water quality was found to be very poor in the monsoon and winteras compared to the summer season. It may be due to less rainfall, siltation and anthropogenic interruption consequently disobeys the norms of potability.Keywords: Anthropogenic interruption, Demand parameter, Jayakwadi dam, Siltation.

Effect of Power Loom Sound on the People Residing in Bhiwandi region of Thane District

Kamran Abbas Mirza, Shaikh Nasreen, Shaikh Mishquat and Moses J. KoletK.M.E. Society’s G.M. Momin Womens’ College, Bhiwandi, Thane, Mahrashtra, India

Abstract: The power looms of Bhiwandi have flourished since early 20th century. The era of looms from Hand looms to Power looms has seen vast advancements in both business and inflow of gene pool. The people from all over the country have migrated to Bhiwandi to try their fortune in the business of power looms. The ill part comes in when the people do not think of the problems they face while residing around the Power Looms. The major issues faced by them are continual exposure to sound over 80 to 89 decibels, insomnia, hypertension, and speech disorders. This has widely created a tremendous psychological disturbance in the pattern of living.Keywords: Decibels, Insomnia, Hypertension

ISBN: 978-93-5382-353-5


Page| 39

Nanoparticles as Effective Adsorbent for Removal of Heavy Metals from Aqueous Solutions

Chandranayan Waghmare1, Kiran Pakhale1, Sayyed Hussain1 and Shivanand Sonkamble2

1Post Graduate Department of Chemistry, Sir Sayyed College, Aurangabad2Department of Chemistry, Pratisthan College, Paithan, Aurangabad

Abstract: Advances in nanoscale science and engineering encourage resolving various recent problems associated with water quality using nanosorbents and nanoparticles resulting from the development of nanotechnology.The resulting products involving nanotechnology helps to decrease the concentration of harmful toxic compounds to tolerable level. This can assist to achieve standard water quality as per health advisories.The present work is to study the possibilities on the removal of Ni ions from aqueous solutions using Copper oxide nanoparticles as adsorbent. Removal of Niions were investigated using solutions with different concentrations in the range 20 to 150 mg/L. In this study, removal of Niions on surfaces has been investigated byspectrophotometric method. Batch adsorption studies were performed as a function of contact time, initial heavy metals concentration, and pH. The heavy metals sorption has been well explained using Langmuir isotherm model.Keywords: Copper oxide nanoparticles, adsorption, water

Biological and Pharmacological Properties of Schiff Base Metal Complexes Derived from Subsituted Pyridine, Aminopyridine and

Aminopyrimidine– A ReviewD. T. Sakhare1 and A. G. Shankarwar2

1U.G. & P.G. Department of Chemistry, Shivaji, Art’s, Comm. & Science College, Kannad2U.G & P.G. Department of Chemistry, S.B.E.S. College of Science, Aurangabad

Abstract: Schiff bases are aldehyde or ketone like compounds in which the carbonyl group is replaced by an imine or azomethine group. They are widely used for industrial purposes and also exhibit a broad range of biological activities. Aromatic aldehydes and aliphatic or aromatic ketone gives stable azomethine moiety. So far, modifications of the Schiff bases have proven highly efficient with improved potency and lesser toxicity. Schiff base metal(II) complexes of various donor atom of Substituted pyridine, amonopyridine and aminopyrimidine based ligands posses anti-tumor and anti-viral, anti -malarial, anti -fungal and anti microbial activities. This review focuses on pharmacological properties of Schiff base metal(II) complexes derived from Substituted pyridine, aminopyridine and aminopyrimidine.Keywords: Pyridine, amonopyridine and aminopyrimidine, aldehyde, antimicrobial, antipyretic, antioxidant

ISBN: 978-93-5382-353-5


Page| 40

Lignite Minings of Gujarat and Their Environmental Effect with Mitigation Techniques

Parmar Ishaan, Parmar Chirag and C. P. MistryDepartment of Earth & Environmental Science, KSKV Kachchh University, Bhuj, Kachchh,

Gujarat

Abstract: Lignite an inferior variety of coal constitutes one of the most important mineral resources of Gujarat state, and its exploitation has provided a major portion of revenue in the mineral sector. Gujarat having being located quite far away from the various coal field of India, in the last two decades, the lignite has been found as cheaper alternative of energy resource and numerous industries in Gujarat have switched over to lignite as a source of energy. Beds of lignite occur in reasonably rich deposits in Kachchh, Saurashtra and south Gujarat. Lignite is early form of fossil fuel derived from decomposed plant and vegetable matter in the same manner as coal, but not as matured as coal. As organic matter is buried and compressed, the amount of water, oxygen, and hydrogen decreases, producing an increasingly carbon-rich material that grades from: Peat to lignite, then bituminous coal, and finally anthracite. As a solid fuel lignite exhibits all the properties of coal. Therefore lignite has to play a very important role as a substitute for coal.The three lignite mining in Gujarat occurs in Kachchh, Saurashtra and south Gujarat. Water samples were collected from different locations in and around the Rajpardi mine site, at South Gujarat, and Panandro mine site at Kachchh were analyzed for different parameters like total hardness (calcium hardness & magnesium hardness), chloride, SO4, TDS, suspended solids, chemical oxygen demand, biological oxygen demand and electrical conductivity. Based on the study, it is found that the ground water and surface water are contaminated with various acids for which some mitigation techniques are suggested.Uses: lignite used as a fuel in power sector, chemical, soda ash, ceramics & tiles, bricks, textiles, cement and othersKeywords: lignite mining, fossil fuel, geology

ISBN: 978-93-5382-353-5


Page| 41

Conservation of Riverine Ecosystems with Special Reference to Central Narmada Riverscape

Vipin Vyas¹ and Parul Gurjar²¹Department of Bioscience, Barkatullah University, Bhopal 462 026 India

²Department of Environmental Science and Limnology, Barkatullah University, Bhopal

Abstract: In last few decades Central Indian River Narmada has witnessed many ecological changes due to increasing human interference. The present paper discuss in details about the changes took place in last few decades in the catchment area of the river and fragmentation of habitats due to formation of small, medium and large reservoirs. For the present study we selected the central stretch of the river starting from the confluence of Dudhi to Indira Sagar reservoir. We selected five sub-basins of major tributaries in this riverscape and prepared their thematic maps with the help of GIS. Remote Sensing data was also used to prepare land use maps and change in land use has also been detected. Physical habitat mapping was also done using the Rapid Bio assessment Protocol. Mesohabitats present in these tributaries were also mapped and documented during the study.The sub-basins of the tributaries namely Dudhi, Barna, Ganjal, Sip and Jamner were selected for the present study. During the study it was found that since most of the tributaries are in hilly slopes, they have a series of runs and riffles but very few pool habitats are available in these streams. Physical habitat assessment indicates that four sub-basins namely Ganjal, Barna, Sip and Jamner are in optimal category whereas Dudhi sub-basin falls in marginal category indicating its deteriorating condition. Substrate categories indicate that sand is the dominant substrate type in Dudhi whereas Barna and Jamner are dominated by cobble substrate type. Ganjal has gravel and Sip has boulder substrate type in domination. Landuse and landcover change has also been detected in these sub-basins for the last few decades. Fragmentation of habitat and disruption in flow is another important factor in this riverscape as a medium reservoir has been constructed by damming the stream Barna which has created a discontinuation of the stream from the main river. This reservoir has also altered the ecological conditions of the streams of Barna sub-basin as most of the stream stretch is now inundated by the back waters of the reservoir and converted the shallow water habitats into deep water habitats. Keywords: Stream ecosystem, landuse-landcover, catchment, fragmentation of habitats

ISBN: 978-93-5382-353-5


Page| 42

Quality of Lignite of Rajpardi Mine and Their Environmental Impact with Management Plan, Bharuch District, South Gujarat

C. P. Mistry, Parmar Chirag and Parmar IshaanDepartment of Earth & Environmental Science, KSKV Kachchh University, Bhuj, Kachchh,

Gujarat

Abstract: Gujarat is rich in lignite, bauxite, fluorite, bentonite, limestone, marble, dolomite and granite. Lignite is an inferior variety of coal; numerous industries in Gujarat have switched over to lignite as a natural source of energy. Lignite is one of the most important mineral resources of Gujarat state, and its exploitation has provided a major portion of revenue. Gujarat being located far from the various coal fields of India, the lignite found here happens to be the cheapest alternative of energy resources. Lignite seams occur in the lower Tertiary strata and form reasonably rich deposits in the South Gujarat, Saurashtra and Kachchh. As a solid fuel, lignite exhibits all the properties of coal. The present study reveals quality of lignite of Rajpardi mine and impact of mining in relation to geology as well as to the environment with management plans. Rajpardi is a village in district of Bharuch, South Gujarat. There are various types of clays, shales and carbonaceous rocks associated with the lignite beds of Tertiary age. The composite lithologs are prepared and studied of the mining site. Petrographical studies are carried out of the lignite and other shales of the studied region. Water samples were collected from different locations in and around the Rajpardi site and were analyzed for different parameters like total hardness (calcium hardness & magnesium hardness), chloride, SO4, TDS, suspended solids, chemical oxygen demand, biological oxygen demand and electrical conductivity. Soil samples from different locations were collected and analyzed for different environmental parameters. Environment management plan (EMP) for Water, Soil, Noise, Surface water management, Groundwater management, Waste water management, Reclamation of the mined area and post mining environmental management are discussed. Based on the study, some geological mitigation techniques are suggested.Keywords: lignite mining, sedimentary beds, geology, Tertiary age, fossil fuel

ISBN: 978-93-5382-353-5


Page| 43

Journey to the Sixth Mass ExtinctionSambodhi Uttam AmbhoreNess Wadia College, Pune

Abstract: The Earth as we know today is the result of millions of years of history of disasters, evolution, speciation and competition between the livings. There have been ups and downs, species evolved and lost at the rate completely decided by the nature. The history thus tells us about the major downs being the five Mass Extinctions referred to as Big Five. However, the evolution of a particular species challenged the ways of nature. The traditional constraints of habitat, climate, topography and boundary were meaningless. Starting from one corner they reached every possible part of the world. The species named itself Homo sapiens, similarly naming everything along the way thus beginning the anthropogenic era of earth.Humans have caused a change of magnitude which results in loss of major biodiversity all over the world. One weedy species has unwittingly achieved the ability to directly affect its own fate and that of most of the other species on this planet. Humans and their highly evolved brain brought about new concepts in the use of nature’s resources giving rise to agriculture, domestication and industrialisation which has brought us to the point we are, but at a huge price.This change hasn’t gone unnoticed and given rise to the haunting question,” Are we on the journey to the Sixth Mass Extinction?” In the last few decades, scientists have noticed catastrophic decline in numbers of species due to overexploitation, climate disruption, habitat loss, hunting, poaching and everything that comes along with the package of what is “Being Human”. Researchers using a sample of 27,600 terrestrial vertebrate species and more detailed analysis of 177 mammal species showed extremely high degree of population decay in vertebrates, even in common species of low concern. This study of biological annihilation is studied based on a quantitative standpoint revealing that the matters at hand are severe that perceived. The IUCN red-list lists about more than 27000 species as threatened with extinction which includes 40% amphibians, 25% mammals, 34% conifers, 14% birds, 31% sharks and rays, 33% reef corals and 27% selected crustaceans. So as to analyse what went wrong, we can narrow it down to the four horsemen of the ongoing sixth mass extinction. These include the spread of non-native species all over the world by a single species, the rise of humans to the position of super-predators, being a massive force in directing evolution and the development of “Technosphere”.Now, the question that first raises panic in the brains of us selfish species is, will we survive this extinction? Will we pull through? My answer is maybe. We as a species have proven to be resourceful so we just might, but what we really need to ask ourselves is as Elizabeth Kolbert said “Do we really want to find the answer to that?”Keywords: Extinction, Anthropogenic, Biodiversity, Haunting, Vertebrates, Amphibians, Super-predators

ISBN: 978-93-5382-353-5


Page| 44

Comparitive Study on Physico-Chemical, Demand Analysis and Bacteriological Analysis of Harvested Rain Water & Non Harvested

Ground WaterSuparna Deshmukh

S. K. Gandhi College, Kada, Beed

Abstract: Rain Water Harvesting, an effective remedy on water crisis, is a method of recharging the groundwater and reducing the runoff water and water logging. It is a multipurpose way of supplying water in which reserved rainwater on rooftops can be used for self-purposes or domestic use by storing .In many states where it has made mandatory, the implementation has become successful in water conservation and water shed management. Kada is a village which lies in district Beed of Maharashtra State in India, and is a strongly drought prone area. It is in this concern, present studies have undertaken for conservation of natural rain water and its storage in surface water or in tubewells. By constructing Rain Water Harvesting systems at five different locations in Kada, which included schools and some crowdy public places, researcher has found successful rise in water levels of borewells and people are enjoying the results. The present studies are also carried out to find the water quality and purity of harvested rain water and it is compared with water quality of non harvested ground water in the same vicinity. At six different sites water samples were assessed for physico-chemical analysis and at ten sites for bacteriological analysis. The different physicochemical parameters studied are pH, Turbidity, Chlorides, Nitrtes, Sulphates, TDS, Hardness, Alkalinity, Iron and Fluoride. Demand Analysis results revealed that, DO content Increases while BOD and COD values decreases in harvested rain water.Researcher assessed potability as per IS 10500 for drinking water quality assessment, and comparative studies have shown that water gets more purified naturally when rain water is harvested.Keywords: Water crisis, Rain water harvesting, Physico-chemical analysis, Bacteriological analysis, Demand Analysis

ISBN: 978-93-5382-353-5


Page| 45

Compassion Meditation: Neuroscience, Quantum Mind and Environmental Changes

Aman Ghutke1, Kshama Khobragade2, Bhikkhu Ananda3

1Research Student, S.B.E.S. College of Science, Aurangabad, 2Associate Professor and Head (Dept. of Environmental Science), S.B.E.S. College of Science, Aurangabad, 3Director,

International Buddhist Meditation Vihara and Research Centre

Abstract: Compassion meditation is consistent with the concern for environment. It is known that there is a close relationship between morality, meditation and wisdom and therefore, with natural environment. All beings are dependent on food, clothes, shelter, medicine and all other concerned needs for survival. The beings can survive only when they understand nature perfectly. Compassion meditation not only purifies one’s own self but it purifies natural environment through our right intentions and actions. The textual records of the Dhamma testifies the importance of forests, rivers and mountains with respect to conservation. The law of conservation of mass and the law of conservation of energy in this regard, if applied to quantum mechanics and quantum neuroscience the environmental crisis could be dramatically be changed and equilibrium can be established. The profit oriented corporations as against the public oriented compassionate beings, if are made strong, it could reverse the process of environmental degradation. Therefore let the compassion work against the cravings neuro-dynamically with the effect that neural network in the brain may bring the changes in the environment with positive actions done with mindfulness to control the environmental pollution and conserve the environment.Keywords: Environment, Meditation, Neuroscience, Quantum mind.

Kinetic Study of the Reaction of 5-Chlrosalicyaldehyde with p-Chloro aniline Spctrophotomerically

Bhausaheb K. Magar, D. T. Sakhare and V. N.BhosaleDepartment of Chemistry, Shivaji Arts Commerce and Science College, Kannad, Aurangabad,

Maharashtra.

Abstract: The second order reaction rate constant for the reaction of 5-chorosalicyaldehyde with p-Chloro aniline have been reported in ethanol at temperature range 303 to 318 k .The rate of reaction is first order with respect to 5-chorosalicyaldehyde and p-Chloro aniline. The rate of reaction increases with increases in temperature. The thermodynamic parameters are used to explain the nature of reaction. Suitable reaction mechanism has been suggested for the formation of the Schiff base. From the effect of temperature on the rate of reaction various thermodynamic parameter have been evaluated. Keywords: Schiff base, 5-Chlorosalicylidene-p-Chloro aniline, kinetics.

ISBN: 978-93-5382-353-5


Page| 46

Environment Friendly Packaging for Dairy ProductsKhojare A. S.

Department of Dairy science and Technology,Vivekanand Arts, Sardar Dalipsingh Commerce & Science College, Samarthnagar, Aurangabad, Maharashtra

Abstract: With an increasing concern towards environment due to over use of plastics in packaging of dairy foods, the bio-packaging is emerging as a viable alternative for plastics. Bio-packaging involves use of bio-degradable, edible and environment friendly packaging materials which can protects the food product from environmental parameters as well as enhance its shelf life due to its antibacterial properties. In near future bio-packaging may offer the complete solution to replace the plastic or minimize use of it by giving added internal protection to food. With changing dimension of Indian dairy industry it is necessary to use and incorporate the necessary technologies for shelf life enhancement, product monitoring and packaging of dairy products. As far as Indian milk products are concerned, it is mostly confined to unorganized and small scale industries. Very few Indian dairy products have been taken up in the past by bigger dairy brands in India. The reason behind this is non standardization of production processes and unavailability of reliable scale-up data to upgrade the production at mass level. There is a real need to generate rational data on biopackaging and shelf life extension of indigenous dairy products. The present studies communicate some approaches for environment friendly packaging for dairy products.

Synthesis and Characterization of Heteropoly Acids and Its Application Inorganic Reactions

Kiran Khillare, Dipak S. Aherand S. G. Shankarwar

Abstract: Heteropolyacids (HPAs) possess unique properties like Bronsted acidity, possibility to modify their properties by changing their chemical composition, high proton mobility, etc. Synthesis of Heteropolyacids done by etherate method. The synthesized catalysts were characterized by SEM-EDAX, FT-IR, TG-DTA, and XRD. In the support of green chemistry, the substitution of harmful liquid acids by solid reusable HPAs as catalyst in organic synthesis is the most promising application of this acids. Synthesized Heteropolyacidsused as acid catalyst in many organic reaction shows that HPAs can carry out reactions at 500 to 800 C temperatures in short time. The application of Heteropolyacids as catalytic materials is growing continuously in the catalytic field.Keywords: Heteropolyacids, Green chemistry, catalysis

ISBN: 978-93-5382-353-5


Page| 47

Development of in Vitro Drug Release Methods for Doxorubicin Nano Pharmaceutical with Different Techniques

Shripad Deshpande1, Mazahar Farooqui2, Parag Deshmukh3 and D. D. Kayande1

1S.B.E.S.College of Science, Aurangabad, Maharashtra2Dr. Rafiq Zakaria College for Women, Aurangabad, Maharashtra

3ICICI Knowledge Park, Shameerpet, Hyderbad

Abstract: In the past few years, measurement of drug release from pharmaceutical dosage forms has been a focus of extensive research because the release profile obtained in vitro cangive an indication of the drug’s performance in vivo. Drug release behavior is an important factor related to drug stability and therapeutic results. Currently, there are no compendial in vitro release methods designed for liposomes owing to a range of experimental challenges, which has created a major hurdle for both development and regulatory acceptance of liposome-based drug products. In this paper, we develop and used the techniques to assess in vitro drug release from PEGylateddoxorubicin prepared in laboratory and doxorubicin liposomal products available in market for comparative study; these include the membrane diffusion techniques, (dialysis membrane (DM))the sample-and-separate (SS)approach, the continuous flow (CF),rotating bottle apparatus (RBA) The SS method allows direct measurement of drug release with simple set-up requirements, but sampling is cumbersome. With the CF method, sampling is straightforward but the set-up is time consuming. Set-up as well as sampling is easier with the DM, A new method that involves the use of cation exchange resinDowex-50 also gives the 85% drug release in 24 hours, while the SS techniques gives more than 85% release in 8 hours at 45°C. Different buffer concentrations, temperature and variation in pH of buffer show the significant changes in drug release. Keywords: dialysis membrane; in vitro release; liposomes; Dowex-50; sample and separate.

Preserving Ecological Richness through Constructionism MaroliaKautuk and Thengodkar RRM

Cyanofarm Research Centre, UVSS, Keshavsrushti, Uttan, Thane

Abstract: In the present day situation, with expanding populace and industrialization; the characteristic flora-fauna are disturbed. A portion of the animal species are getting jeopardized which is leading to natural adversities like climatic changes, sea acidification, storms, droughts, etc. additionally influencing the ecological balance by creating an obstacle in the food chain and affecting other life forms significantly. A considerable lot of creatures are legitimately or by implication identified with human prosperity. Considering the simple example of an earthworm or even a praying mantis, they play a significant role in horticulture and economy in agricultural nations like India. Keywords: Ecological conservation, Biodiversity, ecosystem functioning

ISBN: 978-93-5382-353-5


Page| 48

Study to Improve the Quality of Tannery Effluent for Chemical Oxidation Process Using Sodium Hypchlorite

Ganpat B. More, Shridhar K. Jadhav and Sanjaykumar R. ThoratSchool of Environmental and Earth Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra

University, Jalgaon

Abstract: In the present investigation, the chemical oxidation process was used including sodium hypo-chloride for the pre-treatment and for tertiary treatment in the tannery effluent treatment plant to improve the quality of the effluent. Based on range of COD in treated effluent / raw effluent, necessary dose of sodium hypochlorite was provided to achieve the expected treatment efficiency to meet the discharge norms. Rate of oxidation increases as dose of Sodium Hypochlorite increases. Light yellow coloured effluent turns into very light lemon yellow coloured effluent with crystal clean appearance. Based on dose and retention time COD reduction was achieved from 10 % to 50%. The study shows that the treatment Trial of treated effluent of ETP: COD reduction received from 30 to 50%. Treatment Trial on condensate/distillate at 70 degree Celsius: COD reduction from 7 to 37%. Treatment of chemical oxidation on High COD bearing condensate /distillate. After calculating quality of this stream 15 to 20 kl /day, the COD of composite waste was reduced by 1000 mg/L which has certainly help to improve the quality of treated effluent. This treatment gives an excellent result so that finally treated effluent shall meet the discharge norm of COD in tannery effluent.Keywords: Tannery effluent, Sodium hypo-chloride, COD reduction and Rate of oxidation

Synthesis, Characterization and Application of Vanadium Substituted Keggin-Type Heteropoly Acid

Dipak S Aher, Kiran Khillare, S. G. Shankarwar

Abstract: An efficient, Novel, and recyclable vanadium substituted ternary heteropoly acid has been synthesized and supported on naturally occurring montmorillonite by wet impregnation method. The Catalyst was fully characterized using FT-IR, XRD, SEM-EDS, TGA-DTA analysis. The catalytic application has been explored for the synthesis of tetrahydrobenzo[b] pyrans derivatives were found in excellent yield in a shorter time period. The study demonstrated that newly synthesized heteropoly acid has been successfully incorporated on montmorillonite with their good performance and activity.Keywords: Heteropoly acid, Montmorillonite, tetrahydrobenzo[b] pyran

ISBN: 978-93-5382-353-5


Page| 49

Carbon Sequestration Dynamics in Selective Tree Species Grown in Kavayitri Bahinabai Chaudhari North Maharashtra University

Campus, JalgaonShridhar K. Jadhav,Ganpat B. Moreand Sanjaykumar R. Thorat

School of Environmental and Earth Sciences, Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon

Abstract: The study describes CO2 fixV.2 model, a user-friendly tool for dynamically estimating the carbon sequestration of forest management, agroforestry and afforestation. In the present investigation, total carbon sequesters in trees and the campus of Kavayitri Bahinabai Chaudhari North Maharashtra University, Jalgaon, M.S. India is conducted. The biomass and total organic carbon of standing trees was estimated by non-destructive method. The tree height and girth are taken in to consideration for the measurement of biomass and carbon content. The height of tree is measured by using theoretical model and the concept was used for actual measurement of 20 different tree species having diameter >10cm. CO2 fixV.2 model is a valuable tool to improve the estimate of the carbon mitigation potential of study area including ARD. At the time of estimation of organic carbon storage by model can success used to determine the tree biomass by non-destructive method. Keywords: CO2 fixV.2 model, Biomass, Carbon sequestration and Carbon storage

Synthesis of Dihydropyrimidin-2(H)-one Derivatives via Resin Functionalized Diisopropylethylamine-Catalyzed Biginelli ReactionMahendra A. Chaudhari1, Deepak Kayande1, Jitendra B. Gujar2 and M. S. Shingare2

1S. B. E. S. College of Science, Aurangabad2Department of Chemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad

Abstract: A series of dihydropyrimidin-2(1H)-ones has been synthesized using ultrasound irradiation technique in excellent yields through a resin functionalized diisopropylethylamine catalyzed one-pot, three component Biginelli reactions of aldehydes, β-ketoesters and urea under solvent-free conditions at 80 oC. PS-DIEA is a highly efficient, eco-friendly catalyst and could be recovered and reused several times without significant loss of its activity. The notable advantages of this method are a shorter reaction time, mild conditions, and applicable to broad range of aldehydes, and easy work-up procedure. Keywords: Green chemistry, dihydropyrimidin-2(1H)-ones, resin functionalized diisopropylethyl-amine

ISBN: 978-93-5382-353-5


Page| 50

Soil Health from Irrigated, Non-irrigated and Semi Irrigated Areas of Sangamner Tahasil in Ahmednagar District

Sangita DandwateDepartment of Chemistry, S.M.B.S.T. College, Sangamner

Abstract: The soil sample from Irrigated, Non irrigated and Semi irrigated Areas of Sangamner Tahasil, in Ahmednagar district (M.S.) were analysed for selected physico chemical parameters to know their fertility status. For this purpose 45 soil samples were collected from 45 villages of Sangamner Tahasil.On the basis of these values average valuesof parameters analysed. The chemical soil properties viz pH, electrical conductivity, calcium, magnesium, sodium, calcium carbonate, organic carbon, organic matter and available NPK from irrigation area, non-irrigated area and semi irrigated areas were studied in summer 2017 .The soil pH and electrical conductivity were higher in irrigated area (8.21 and 1.36 dSm-1) and semi irrigated area (8.13 and 1.11 dSm-1) than the non-irrigated area (8.08 and 0.79 dSm-1). Organic carbon and organic matter content of soil from the non-irrigated area was higher as compared to other two areas. The nutrient fertility status with respect to available nitrogen content was low in irrigation, non-irrigated and semi irrigated areas 157.51, 178.46 and 177.38 kgha-1 respectively. Also the nutrient fertility status with respect to available Phosphorous content was low in irrigation, non irrigated and semi irrigated areas .The available potassium were in higher fertility class in irrigation area ( 531.68 kgha-1), non-irrigated area (300.67 kgha-1) and semi irrigated 498.14 kgha-1 The soil from area were clayey to clay loam textural class and maximum water holding capacity The higher nutrient fertility status in lift irrigation fields might be associated with intensive cultivation and plantation of cash crops like sugarcane, cotton, fruit crops etc. in which use of fertilizers as practiced by the cultivators. Keywords: Soil health, physico-chemical properties, Irrigated, Non irrigated and Semi irrigated, Sangamner

ISBN: 978-93-5382-353-5


Page| 51

Removal of Pollutants by Root Zone Treatment Technology from Domestic Sewage

Jadhav P. S., Khobragade K. S. and Ghutke A. V.Department of Environmental Science S.B.E.S. College of Science. Aurangabad

Abstract: Water is essential for human survival and well-being and important to many sectors of the economy. However, resources are irregularly distributed in space and time, and they are under pressure due to human activity. Water pollution in India is a major problem. In recent years because of industrialization and urbanization in India, this problem has become enormous. Due to the various human activities the quality and quantity of surface and underground water resources is changing. The change in quality of surface water changes the composition of natural water.Urban water supply and sanitation are important basic needs for the improvement of the quality of life and enhancement of productive efficiency of the people. In urban areas, water is tapped for domestic and industrial uses from rivers, streams, wells and lakes. Almost 80% of the water supplied for domestic use, comes out as wastewater. In most of the cases wastewater is let out untreated and it either sinks into the ground as a potential pollutant of ground water or is discharged into the natural drainage system causing pollution in downstream areas.Nowadays natural wastewater treatment technologies gain more significance. Root zone treatment technology for domestic sewage wastewater treatment has been proven to be effective and sustainable alternative for various conventional wastewater technologies.The objective of this study is to install pilot scale Root zone treatment system to treat domestic sewage by using an aquatic macrophyte Phragmites Australis. In the present investigation water samples were collected seasonally for the period of one year and treated it with Root zone treatment system, both influent and effluent samples were taken for analysis. Maximum COD reduction found 77.22 % in winter season and minimum COD reduction was observed 53.80 % in the summer season. Maximum BOD reduction found 72.83 % in the winter season and the minimum BOD reduction found 59.66 % in the summer season. The maximum total phosphorus reduction found 55.88% in winter season and minimum total phosphorus reduction was observed in the summer season. Nitrate (NO3

-N) is one of the significant pollution indicating parameter. Maximum percentage of Nitrate reduction was observed 56.64 % in winter season and minimum Nitrate reduction was found 43.50 % in the summer season.The efficiency of Root zone treatment system to remove the contaminants from the wastewater mainly depends on the root zone interactions between soil, contaminants, helophyte roots and a diversity of microorganisms. The soil is the main supporting material for plant and microbial growth.Keywords: Pollutants, Biodegradation, Domestic sewage, Phragmites Australis, Root zone treatment technology

ISBN: 978-93-5382-353-5


Page| 52

Muncipal Solid Waste: Disposal and RecoveryA. M. Raydurg and B.S.Inamdar

Anjuman Arts, Science & Commerce College, Vijayapur

Abstract: Solid wastes released by cities are referred as municipal solid waste(MSW).Solid waste often called the third pollution after air and water pollution, it is that material which arises from various human activities and which is normally discarded as useless or unwanted.It consist highly heterogeneous mass of discarded materials from the urban community as well as the more homogeneous accumulation of agricultural industrial and mining wastes. In India, several tonnes of solid waste is dumped along highways and other places in large cities as Delhi,Mumbai,Kolkatta,Channai,Ahmedabad,Jaipur etc. There is problem of disposal of these wastes especially in developed countries where labour is very expensive. To solve these problems technologies have been developed to recycle most of the solid waste items. Thus paper cans, newspapers, and other waste paper may be easily recycled. Metallic components of vehicle spares may be recycled by cheap methods. There is problem of recycle of heavy metals, plastic, nylon, polythene etc.Keyword: Muncipal Wastes, Disposal, Waste management, Garbage basins open dumping, Ocean dumping, Sanitary land, composting, Re-cycling of waste

Co-Biodegradation of Naphthalene and Phenanthreneby Bacteria Isolated from Hydrocarbon Contaminated Sites

Vivek Parab and Manju PhadkeDepartment of Microbiology, SIES College of Arts, Science and Commerce, Sion (West), Mumbai,

University of Mumbai

Abstract: Polycyclic aromatic hydrocarbons (PAH) are categorized as environmental pollutants which are potentially toxic to human health system. The most commonly found PAHs in environment are Phenanthrene and Naphthalene which in large amounts are carcinogenic and hence are considered harmful. . The present study indicates that bacterial isolates were able to degrade PAH at a higher rate. The isolates were identified by 16s rRNA sequencing method. The isolates able to produce biofilm which were used to develop a bacterial consortia. A laboratory scale biofilm bioreactor was developed for treatment of contaminated water. To further enhance the growth rate of organisms during degradation, a natural surfactant soapnut solution and a chemical surfactant Tween80 were tested as surfactants and their activity was compared.The study further revealed that the consortia formed using the isolates was able to degrade a high concentration of Phenanthrene and Naphthalene and was able to reduce the Chemical Oxygen Demand (COD) of water contaminated with PAH. Keywords: Biodegradation, Bioremediation, Phenanthrene, Polycyclic aromatic hydrocarbons

ISBN: 978-93-5382-353-5


Page| 53

The Effect of Bis-Bindent Carboxylic Groups of Squaraine Sensitizers on the Efficiency of Dye-Sensitized Solar Cells

Sultan A. Al-horaibi, Suresh T. Gaikwad and Anjali S. RajbhojDepartment of Chemistry, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad,

Maharashtra

Abstract: Two new symmetric squaraine sensitizers (SQTHZ and SQIND) carrying benzothiazole and indoline moieties as strong electron donating groups to inject the electron into the TiO2 nanoparticles were tested as DSSC. The theoretical calculations and absorption results show that the electron density of LUMO of SQTHZ is delocalized in the whole chromophore, leading to strong electronic coupling between SQTHZ sensitizer and the conduction band of TiO2. Furthermore, the presence of long alkyl chain with pendent bis-SO3

- groups would inhibit recombination and decrease the dye aggregation. Interestingly, SQTHZ displayed UV-Vis and NIR absorption at a longer wavelength compared to SQIND. This structure feature, as well as optical properties, would lead to improved efficiency of dye-sensitized solar cell with overall better photovoltaic performance (n of 3.31%, Jsc of 7.65 mA/cm2, Voc of 0.59, ff of 0.71 and IPCE of 47% at 674 nm) compared to SQIND.Keywords: Dye-sensitized solar cell, Symmetric squaraine dyes, Photoelectrochemical properties, DFT

Biobeds: An Effective and Eco-Friendly Method for Disposal of Pesticide Waste

R. Y. Bhandare1, P. R. More2, T. S. Pathan3 and S. E. Shinde4

1Dept. of Zoology, MGV’s Arts, Science and Commerce College, Surgana, Nashik2Dept. of Zoology, Kai Rasika Mahavidyalaya, Deoni, Latur

3Dept. of Zoology, Kalikadevi Arts, Commerce and Science College, Shirur (K. A.) Shirur4Dept. of Zoology Maharaja J.P Valvi Arts, Commerce & Shri V.K Kulkarni Science College

Dhadgaon

Abstract: The pesticides are one of the tools for achieving the goal of the green revolution and play an important role in improving yield of various crops. Though the pesticides play a vital role in controlling crop pests, it results in to pollution of environment especially land and water bodies. Pollution occurred mainly due to large amount of pesticides and chemicals used in the farm, and now a day the use of pesticides and chemicals in farm is very common. Generally soil gets polluted by improper disposal of pesticides and chemicals. Therefore there is need to degrade the pesticide by using a bio bed technique. This technique is most effective method to degrade the pesticide.Keywords: Bio bed, Pesticide, Chemicals, Pollution

ISBN: 978-93-5382-353-5


Page| 54

To Study the Flood Affecting Area from Aurangabad District by Using Remotely Sensed Data

Eman MA. Garoon1, Sultan AH. Al-horaibi2 and M.B. Mule1

1Department of Environmental Science, 2Department of Chemistry, Dr. Babasaheb Ambedkar 2Marathwada University, Aurangabad, Maharashtra

Abstract: The Aurangabad district has geographical area of 10,107 sq. km. out of which 814.15 sq. km is occupied by forest whereas cultivable area is 8135.57 sq. km and net area sown is 7150.55 sq. km in 2005-06. Agriculture is the main occupation of the rural people. The conventional means to record hydrological parameters of a flood often fail to record an extreme event. Remote sensing technology along with geographic information system (GIS) has become the key tool for flood monitoring in recent years. Development in this field has evolved from optical to radar remote sensing, which has provided all weather capability compared to the optical sensors for the purpose of flood mapping. The central focus in this field revolves around delineation of flood zones and preparation of flood hazard maps for the vulnerable areas. In this exercise flood depth is considered crucial for flood hazard mapping and a digital elevation model (DEM) is considered to be the most effective means to estimate flood depth from remotely sensed or hydrological data. In a flat terrain accuracy of flood estimation depends primarily on the resolution of the DEM. River flooding in Aurangabad district is very acute because of their heavy dependence on agriculture but any flood estimation or hazard mapping attempt in this region is handicapped by poor availability of high resolution DEMs. This project presents a review of application of remote sensing and GIS in flood management with particular focus on the Aurangabad district.Keywords: Remote sensing, flood hazard, GIS

ISBN: 978-93-5382-353-5


Page| 55

GROUP- CLife Sciences

ISBN: 978-93-5382-353-5


Page| 56

ISBN: 978-93-5382-353-5


Page| 57

Endophytic Serratia Spp. of V. vinefera: A Treasure House of Biomolecules

Anita V. Handore1, S. R. Khandelwal2 and A. D. Bholay3

1P.G. Department of Microbiology, HPT Arts and RYK Science College, Nashik2H.A.L. College of Science & Commerce, Ozar Township, Nashik

3P.G. Department, Microbiology, K.T.H.M. College, Nashik

Abstract: Endophytes are the important microorganisms that grow intra-and/or intercellularly in the plant tissues and acts as reservoir of numerous bioactive substances. Generally; Serratia Spp. shows productionof prodigiosin, a secondary metabolite exhibiting promising antibacterial, anti-fungal, anti-neoplastic, anti-proliferative, anti-oxidant and anti-malarial activity. Since the endophytes resides the host plant and mimics the properties of its host, present research has been focused to isolate endophytic Serratia spp. from the medicinally important V.vinefera i.e. Grapevine plant and different activities of this endophyte for various ecofriendly and cost effective applications. Serratia spp. was isolated from different plant parts of the black cultivar of V.vinefera. Biosynthesis of Silver nanoparticles were carried out by reaction of freshly prepared 1mM Silver nitrate solution with cell free supernatant ofSerratia spp. (1:5 v/v). AgNPs were characterized by UV-Vis spectra in the range (200-800 nm). Presumptive test for presence of prodigiosin pigment was carried out by using conc.HCl and conc.Ammonia. Antioxidant activity was carried out by DPPH radical scavenging assay. The detection of siderophore was carried out by CAS assay.In present study; it was revealed that the reaction mixture exhibited brown coloration indicating synthesis of AgNPs .The Characterization of AgNPs by UV-Vis spectroscopy exhibited maximum absorbance at 427 nm showing significant potential for biosynthesis of AgNPs. The presumptive test for prodigiosin showed pink and tan coloration in acidic and alkaline condition respectively, confirming the pigment as prodigiosin. The antioxidant activity was found to be 68.3%. It was revealed that Serratia spp. showed noteworthy ability to produce Catecholate type of siderophore. The rate of siderophore production was found to be 39.58% Therefore, the endophytic Serratia spp. isolated from V.vinefera may offer immense scope for their cost effective application in the various fields like Pharmaceutical industry, Agriculture, Textile and Food industry for empowerment of the society.Keywords: Endophyte, V.vinefera, Serratia spp., Siderophore, AgNPs, Prodigiosin, Antioxidant

ISBN: 978-93-5382-353-5


Page| 58

Production and Characterization of Thermostable Amylase from Bacillus Korlensis Isolated from Unkeshwar Hot Spring, Nanded

Anupama Prabhakarrao Pathak1 and Mukundraj Govindrao Rathod2

1School of Life Sciences, S.R.T.M. University, Nanded2Dept. of Bioinformatics & Biotechnology (U.G. & P.G.), Yeshwant College of I.T., Parbhani

Abstract: Efficient thermostable amylase producer Bacillus korlensis was isolated and characterized from Unkeshwar hot spring, Nanded, Maharashtra. It was Gram positive, motile rod and spore former. It has shown acid production from arabinose, Cellobiose, Fructose, galactose, glucose, glycerol, lactose, mannitol, maltose, ribose, salicin, and sucrose. The colonies were pale brown, circular with Shiny glistering and granular surface. It has shown optimum growth at pH 7 and temperature 65oC. Remarkable thermostable amylase production was recorded at 55oC and pH 7 after 24 hour of production period using various agricultural residues. Molecular weight of partially purified amylase was recorded 65 KD. Catalytic activity of partially purified amylase was enhanced in presence of 1 Mm MgCl2, CaCl2 and ZnCl2.Keywords: SDS-PAGE, Identification, Catalytic activity, sugars, Metal cations

Histochemical Analysis of Gastrointestinal Region of Fresh Water Fish Wallago Attu Infected with the Helminth Parasite Gangesia Species

Pathan A.V. and Korde SeemaDepartment of Zoology and Fishery science, Azad College, Ausa, Maharashtra

Abstract: The present study was undertaken to determine the histochemical changes induced by Gangesia Sp. in the intestine of freshwater fish Wallago attu. Histochemical tests are used in an attempt to identify cell and tissue components by virtue of their specific chemical reactions. The alteration in the state of cell constituent can be studied by using histochemical techniques, these techniques helps to analyze not only the localization of carbohydrate, protein, lipid and glycogen etc. but also molecular changes at cellular level. During present investigations the infection of Gangesia Sp. in Wallago attu. with various histochemical reactions showed localization of carbohydrate, protein, lipid and glycogen. During histochemical study intestine infected by cestodes shows the presence acid mucopolysaccharide content and the numbers of mucous cells those containing acidic or mixed glycoconjugates were significantly higher than those seen on sections from uninfected fish, which is a protective interaction of the host against parasitic infection. In the current study, a highly significant increase in the number of mucous cells was seen within the infected intestines of Wallago attu when compared to uninfected counterparts.

ISBN: 978-93-5382-353-5


Page| 59

Parasitic Infections in Live Freshwater Ornamental Fishes from Latur District

Korde Seema and Pathan A. V.Department of Zoology, Azad College, Ausa- 413520, Maharashtra

Abstract: Present study was conducted to investigate the parasitic infections in live freshwater ornamental fishes. Mass mortality of ornamental fishes occurs due to various parasitic infections. During present investigation detected a total of 7 parasites on 7 of the 15 host species examined 3 ciliates (Ichthyophthirius multifil is, Tetrahymena corlissi, Trichodynia sp.), 2 monogeneans (Gyrodactylus bullatarudis, Gussevia asota ), 1 nematode ( Camallanus cotti ), and 1 copepod (Lernaea cyprinacea ). Two hosts, Sumatra barb Coepeta tetrazona and guppy Poecilia reticulate, were infected with 2 species each (I. multifiliis and Trichodina sp., and T. corlissi and C. cotti, respectively. The introduction of transmissible parasites through the import of ornamental fishes may cause serious disease problems in native fish species, which may have negative impact on emerging ornamental fish trade in India.Keywords: Protozoan, Digenean Parasites, Monogean Parasites

Effect of Methanolic Extract of Achyranthes aspera Linn. on the Larvae of Silkworm, Bombyx mori L.

A. N. Khade, C. J. Hiware and P. P. PathareDepartment of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad

Abstract: Some medicinal plants are widely used in the field of agriculture to protect the crops from the insect pests. In the present study the Achyranthes aspera is used to find out the larvicidal and antifeedants activity on the larvae of silkworm, Bombyx mori. The silkworm B. mori L. is used in the study as an experimental model as an insect larvae. The crude methanolic extract of Achyranthes aspera Linn. Seeds, foliar spray was used in different concentration on the leaves of mulberry and fed to fifth instar larvae of Bombyx mori. The growth rate, feed consumption index, approximate digestibility, Efficiency of Conversion of Ingestion (E.C.I.), Efficiency of Conversion of Digestion (E.C.D.) of food and the Mortality rate of the larvae are recorded. The result of plant extract reveals that, there is marked decrease in the Growth rate, E. C. I. and E.C.D. as compare to control group. The feed Consumption rate of the larvae was increased in 25% and 75% concentration; whereas E.C.D. decreased value was observed in 75%. During the overall study period, in experimental group showed less mortality, whereas 30% mortality was observed in 75%concentration. The details are explained in the text.Keywords: Achyranthes aspera, Bombyx mori, methanolic extracts, growth rate, mortality, digestibility

ISBN: 978-93-5382-353-5


Page| 60

Synthesis and Characterization of Gd3+ Doped Ni0.5Co0.5GdyFe2-yO4, to Study the Structural Characterization Can Be Made by Using

Thermogravimetric Analysis (TGA) and Differential Thermal Analysis (DTA) by Sol-gel Technique

Datta B. Pawar1 and L. S. Ravangave2

1Department of Physics S.B.E.S. College of Science Aurangabad2Shri. Sant Gadge Maharaj Mahavidyalaya, Loha, Dist. Nanded

Abstract: It is a simple analytical technique that measures the amount and rate of change in the weight of a material as a function of temperature or time in a controlled atmosphere.It is the most widely used thermal method which can characterize materials that exhibit weight loss or gain due to decomposition, oxidation or dehydration.TGA-DTA data is collected from room temperature to 8000C at a constant heating rate of 100C / min. Series of Nickel Cobalt Nano ferrites substituted with Gd3+ rare earth, with concentration Ni0.5Co0.5GdyFe2-yO4, x= (0.025, 0.050, 0.075, 0.100, 0.125, 0.150) A slow decomposition has occurred between 300 to 6000C due to decomposition of polymeric species.Keywords: TGA/DTA, Sol-gel technique, Ni-Co Nano ferrite

Organochlorine Pesticides Usage in Agriculture-Advantages and Disadvantages in Kannad Region of Maharashtra

Jadhav T. J. and Pawar S. M.Department of Zoology, Shivaji Arts Commerce and Science College, Kannad, Aurangabad

Abstract: The term pesticides cover in a wide range of organic andinorganic compounds including herbicides, insecticides, fungicides, nematicidesetc.Ware (1975).Except thatorganochlorine (Endosulphan, Rogour, Cypermethrin,Monocil,Monocrotophous,B.H.C.etc.) are used as controlled of malaria and typhus. But organochlorine completely banned after 1960 in America and developed countries. Generallya pesticidemust be lethal to the targeted and non-targeted pest controlled in soil, water and aquatic fauna. But Indiscriminant usage of organochlorine pesticides is directly harm full to human, soil content, aquatic animals etc.Here we study of same organochlorine pesticides use in kannad region to increase production in agro- products such as cotton, wheat tomato, sugarcane and green vegetables etc., but same time we also observe the decreasing quality of soil, water, Human health and agro-products. Keywords: Organochlorine pesticides, Agriculture

ISBN: 978-93-5382-353-5


Page| 61

Toxicity and Biochemical Studies of Some Molluscicides, Herbicides Against Macrochlamys petrosa in Amravati District, Maharashtra

Pradeep S. DeshmukhPost Graduate Dept. of Zoology, Govt. Vidarbha Institute of Science and Humanities, Amravati

Abstract: A pest is an organism whose population often increases above a certain level of economic injury and its existence conflict with welfare, convenience and profit of man. Pest control refers to the regulation or management of a species defined as a pest, usually because it is perceived to be harmful to a person’s health, the ecology or the economy. The pest maybe classified as crop pest, house hold pest and stored grain pest. It is observed that the use of chemical pesticides is hazardous to human health, so now a day humans are diverted to follow traditional techniques of pest control. The modern agricultural techniques are beneficial on one hand while on other hand they have serious long-term side effects such as soil erosion, disturbances in mineral uptake by plants, reduced soil fertility and also affect on human health. Traditional methods of pest control are better than modern techniques. A traditional method includes locally available natural things. The land snails and slugs are important to man as a pest because of damage caused in agriculture, horticulture and forestry. The present snail Macrochlamys petrosa is moisture loving and feeding actively during monsoon and winter seasons of the year. For the control purpose of this snail it is essential to understand in detail ecobiology of these snails. The control of Macrochlamys petrosa was checked in the laboratory by conducting experiments under laboratory conditions .This experiments were carried out by using chemical agents like sodium pentachloroplenate, copper sulphate, Mercuric chloride, Zink sulphate and cadmium nitrate for assessing the toxicity of above molluscicides and on other hand traditional methods were also studied by use of neem extract, cow dung and urine, traps etc. Dusting the powder of chemical and natural resources on the snails, mixing it with soil and sprinkling the liquid concentrations of these chemicals and natural extracts on food of snail were tested. It is observed that direct dusting of the powder of chemical on snail and sprinkling the liquid concentration of these chemicals on food of the snail is most effective as compared to mixing the chemical with soil.Keywords: Pests, Molluscicides, Herbicides, Fertilizers, Extracts of Plants

ISBN: 978-93-5382-353-5


Page| 62

Synthesis, Characterization and Biological Activities of Some Novel 3-(1-((5-(4-halophenyl)-1,3,4-thiadiazol-2-yl)imino)ethyl)-4-hydroxy-2H-

chromen-2-onesJoshi H. U.1, Jadhav R. L.1 and Thakur S. V.3

1Department of Chemistry, Swa. Sawarkar College, Beed2Department of Chemistry, R.B. Attal College Gevrai

3Department of Chemistry, Milliya College Beed

Abstract: The chromen-2-ones represent a large group of compounds that have been reported to possess a wide range of biological activities along with anticoagulant action. Recently these compounds have attracted considerable attraction due to their photochemical characteristics. Several substituted thiadiazoles are reported for their biological activities and outstanding pharmacological importance. Present study is focused on synthesis of novel imines derived from 3-acetyl-4-hydroxy-2H-chromen-2-one and 5-(4-halophenyl)-1,3,4-thiadiazol-2-amine. In vitro biological screening effects of the investigated compounds were tested against bacterial species Staphylococcus aurous, Escherichia coli, Salmonella typhi, Bacillussubstilis by Agar cup method andfungal species Aspergillusniger, Penicilliumchrysogenum, Fusoriummoneliforme and Aspergillusflavus by the poison plate method.Keywords: 3-acetyl-4-hydroxy-2H-chromen-2-one, thiadiazol-2-amine, imines, antimicrobial activity

Study of Buccal Cavity Catla catlaPhulwade Durgesh N.1, Hardik S. Ghumnar2 and Gedam Ajit K.3

1Shri SantSavta Mali GraminMahavidyalayaPhulambri, Aurangabad2Adarsh Education Society’s Arts, Commerce and Science College, Hingoli

3Jijamat College of Arts and Science BhendaTq, Nawasa, Dist. Ahmednager

Abstract: The present study showed that the structure of mouth of Catlacatla is wide and slightly upturned. The lips are soft and lower jaw is prominent. The buccal cavity and pharynx are not clearly marked of each other. The buccal cavity is bounded by upper and lower jaw. The upper and lower jaws are edentulous. Tongue is observed to be well developed triangular with thick mucus membrane, which is affixed along the mid dorsal line of the floor of buccal cavity. The gill rakers are thin and long.Keywords: Tongue, gill rakers, mouth, pharynx

ISBN: 978-93-5382-353-5


Page| 63

Synthesis, Characterization and Biological Activities of Some Novel Schiff Bases Derived from 3-acetyl-4-hydroxy-2H-chromen-2-one and

5-(4-aryl)-1,3,4-thiadiazol-2-amineJadhav R. L.1, Joshi H. U.1 and S. B. Ubale2

1Department of Chemistry, Swa. Sawarkar College, Beed2Department of Chemistry, R.B. Attal College Gevrai

Abstract: Chromen-2-onederivatives are one of the most significant families of natural productsand in synthetic organic chemistry. They have been widely used as starting materials or intermediates in the pharmaceutical, perfumery and agrochemical industries. Thaidiazoles are the important class of heterocyclic compounds with significant biological applications. In view of the high degree of bioactivity shown by chromen-2-oneand thiadiazole, the present study is focused on the synthesis of some novel Schiff bases containing chromen-2-one and amino thaidiazole rings. In vitro biological screening effects of the investigated compounds were tested against bacterial species Staphylococcus aurous, Escherichia coli, Salmonella typhi, Bacillussubstilis by Agar cup method and fungal species Aspergillusniger, Penicilliumchrysogenum, Fusoriummoneliforme and Aspergillusflavus by the poison plate method.Keywords: Chromen-2-one, aminothiadiazole, Shiffs base and antimicrobioal activity

Report of Bacterial Blight on Pomegranate from Aurangabad District of Maharashtra

B. D. Pandit and N. A. GhanawatDepartment of Botany, Yashvantrao Chavan Institute of Science, Satara

Abstract: Pomegranate (Punica granatum L.) is very important in international market as table fruit because of its medicinal uses and nutritive values. Its popular varieties viz. Bhagawa, Ganesh, Mrudula is reported from different region of Aurangabad district of MH. Successful cultivation of pomegranate in recent years has met with bacterial blight caused by Xanthomonas axonopodis pv. punicae is a major disease. A survey has been conducted from 2017 to 2018 and to check the incidence of disease in Aurangabad district of Maharashtra (India). The disease symptoms were recorded on leaves, flowers and fruits of the plant. The disease incidence was recorded from different region of Aurangabad district viz. Phulambri, Karmad, Sillod, Gangapur and Paithan. The disease was found to be severe at Mandaki (A.bad), Karmad, Waregaon (Phulambri) and Pachod (Paithan). The study through light on present status of Bacterial blight on PomegranateKeywords: Pomegranate, Bacterial Blight, Xanthomonas axonopodis pv. punicae (Xap)

ISBN: 978-93-5382-353-5


Page| 64

Isolation of Bacteria Associated with Burrowing Wolf Spider, Pardosapseudoannulata

Jadhav S. D., Phartale N. and Acharya K.Swami Ramanand Teerth Marathwada University, Nanded, Maharashtra

Abstract: In the present study,the mechanical transmission of various pathogenic agents such as bacteria by Spider, Pardosapsuedoannulata, has been confirmed. Isolation and identification of bacteria from external surface of Pardosapsuedoannulatahas not been done previously. This is the first report of bacterial infection of spider Pardosapsuedoannulata. In our study of surface bacteria inoculation on spider Pardosapsuedoannulata, Leconostoc mesenteroidesdextranciumand Staphylococcus sciuri had the highest rate of infection. It is observed that Leconostoc mesenteroidesdextrancium has the greatest rate of bacterial surface infection on spider in addition to Staphylococcus sciurihas been associated with serious infections in humans, such as endocarditis, peritonitis, septic shock, and wound infections. Previous studies indicated isolation of S. sciuri and S. lentus from urine sample. Leuconostoc species have been implicated in a variety of infections, particularly in patients being treated with vancomycin. Very little is known about the epidemiology of Leuconostoc infections. They occur naturally on various foods, and gastrointestinal colonization has been demonstrated for patients. Those diseases could be associated with the rice that have been consumed by the human being which has been grown in the paddy field where this spider is found. The wolf spider Pardosapseudoannulata, plays a crucial role in the rice agro-ecosystem and in agricultural fields and a potentially good bio indicator for heavy metal contamination. Despite the risk and benefit of presence of Pardosapseudoannulatain paddy rice field, the symbiotic association of the bacteria to be interesting as it may be playing a vital role in protecting the mango plant against other predators.Keywords: The wolf spider Pardosapseudoannulata, Isolation of bacteria, Staphylococcus sciuri, Leconostoc mesenteroidesdextrancium

A New Species of the Trematode Parasite, Allocreadium khami N. Sp. from the Freshwater Fish, Mastacembelus armatus, Aurangabad

Pardeshi P. R.1, Davne P. M.1 and Hiware C. J.2

1Department of Zoology, S.B.E.S. College of Science, Aurangabad.2Department of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad

Abstract: A new trematode parasite, Allocreadium khami n.sp. were collected from liver of host, Mastacembelus armatus (Lecepede, 1800) Kham river, Aurangabad district, India. The new species A. khami differ from other known species in having distinct characters viz, shape and size of the body, shape, size and arrangement of suckers, position of reproductive organs. Keywords: Trematode parasite, Allocreadium khami n.sp., Mastacembelus armatus, Aurangabad

ISBN: 978-93-5382-353-5


Page| 65

Cultural, Morphological and Biochemical Characteristics of Azotobacter Isolates from the Rhizosphere Soils of Crop Plants Irrigated with

SewageJadhav P. N.1 and L. V. Gangavane2

1Department of Microbiology,Deogiri college,Aurangabad2Soil Microbiology and Pesticides Lab, Department of Botany, Dr.Babasaheb Ambedkar

Marathwada University, Aurangabad

Abstract: A survey of Azotobacter population was done form the rhizosphere and soil of 15 crop plants during different seasons.In the present study morphological and physiological characteristics of 20 Azotobacter isolates were studied. On the basis of different characteristics five species were identified such as , Azotobacter chroococcum, A.paspali, A. insignis, A.vinelandi and A.beijerinkii. This was based upon the Bergey’s Manual of Systematic Bacteriology (Krieg and Terrand, 1978; Thompson and Skerman, 1979).Keywords: Azotobacter, sewage, Rhizosphere, Bergey, s Manual

Study of Socio- Economic Status of Back Yard Poultry Owner to Boost Employment Opportunities in Rural Population

B. N. JadhavAssistant Professor in Zoology, Vinayakrao Patil Mahavidyalaya Vaijapur, Aurangabad

Abstract: Vaijapur Taluka comes under low rainfall zone so people choose to back yard poultry as a subsidiary business to farming. The objectives of present study to investigate prevailing system of backyard poultry.A study conduct to analyse socio- economic status of back yard poultry owner of Vaijapur taluka. We visited 50 back yard poultry owner along with pre planed questionnaires and data were collected through it. The result of study shows that back yard poultry owner had less than one hector land; so many owners are land less. Majority of owner are graduate among these some of trying to pursue higher education. Majority of owners rearing back yard poultry were 30-35 years of age. Majority of poultry owner had small family up to five members. The earning from back yard poultry are generally fulfils the daily need and also helping hands in children’s education. Backyard poultry plays a key role in supplementary income generation, family nutrition and helps to improve living standard of the poorest of the poor in rural. Keywords: Back yard Poultry, socio- economic status, health, nutrition employment

ISBN: 978-93-5382-353-5


Page| 66

Variability Analysis in Ginger (Zingiber officinale Rosc.) Somaclones Using RAPD and ISSR Marker Systems

Gavande Sharda Shivaji1Centre for Plant Biotechnology and Molecular Biology, Thrissur -680656, Kerala

2Kerala Agricultural University, Thrissur -680656, Kerala

Abstract: Thirteen groups of ginger somaclones which include 180 plants in total along with two source parent cultivars (Maran and Rio-de-Janeiro) were evaluated using two molecular marker systems RAPD and ISSR. RAPD analysis using twelve selected primers produced 129 amplicons, 44 were polymorphic with an average of 3.66 polymorphic bands/ primer and a polymorphism percentage of 34.10. In ISSR assay, twelve selected primers produced 122 amplicons, 32 were polymorphic with an average of 2.66 polymorphic bands/ primer and a polymorphism percentage of 26.23.The dendrograms generated based on RAPD and ISSR profiles grouped the somaclones into two separate clusters, with somaclones of Maran in first subcluster of cluster I and somaclones of Rio-de-Janeiro in second subcluster of cluster I. The regenerants from Rio-de-Janeiro calli irradiated with 20 Gy and somatic embryogenic calli irradiated with 10 Gy formed the second cluster.RAPD and ISSR marker systems showed that somaclones derived from cultivar Maran exhibited more variability than Rio-de-Janeiro. In groupwise variability analysis using bulked DNA, the groups RC20 Gy and RSe10 Gy recorded higher variability from source parent cultivar. The variability exhibited in plantwise analysis using three selected primers was found very high (39%) as compared to groupwise analysis (25%). The somaclone RC2Kr1031 of the callus regenerants and RSe1Kr1052 of the somatic embryo regenerants showed more variability exhibiting 59 and 53 per cent respectively from the source parent cultivar Rio-de-Janeiro.Keywords: Bulked DNA, Ginger, ISSR, RAPD, Somaclonal variation.

ISBN: 978-93-5382-353-5


Page| 67

Effect of Temperature Variation on the Life Cycle of a Dipterous Fly Sarcophaga dux.

S. A. Bansode and V. R. MoreDepartment of Zoology, Government Collegeof Arts and Science, Aurangabad

Abstract: Forensic entomology is the branch of forensic science in which information aboutinsects is used to draw conclusions when investigating legal cases relating toboth humans and wildlife. The insects that can assist in forensic entomological investigations includeblowflies, flesh flies, cheese skippers, hide and skin beetles, rove beetles and clownbeetles. Studies on the entomological evidences on the different animal dead bodies from the Osmanabd district of Maharashtra carried out during the year 2013 to 2016. Sarcophaga dux of Sarcophagidae studied throughout this research to observe effect of temperature on its life cycle.Temperature is one of the most important factors affecting fly oviposition activity and rate of larval development. So, temperature considered as the most important factor while estimating the accurate PMI also. Variations in the climatic conditions lead to the differences in decomposition speed. The results of the present study show that warmer temperatures during summer and rainy seasons speeded up the succession by accelerating the development and activity of the maggots. Whereas, cooler temperatures of winters retarded the development and activity of the maggots. As per the result life cycle of S. dux was completed in 361 hrs (15 days, 1hrs.) summer, in winter 493 hrs (20 days, 13 hrs) and435 hrs (18 days, 3 hrs) in rainy season.Carrion decomposition studies conducted in various geographic locations and among seasons within one geographic location are therefore a necessity in developing a baseline data set for use in the field of forensic entomology. To date, no seasonal or regional studies have been conducted in this area. This affords an excellent opportunity to make a significant contribution to the baseline database used in forensic entomology. Keywords: Carrion, decomposition, forensic, entomology, succession, Dipterous

ISBN: 978-93-5382-353-5


Page| 68

Curative Role of Caffeine (1, 3, 7-Trimethylexanthine) on Lead Induced Alterations in Glycogen Content of Different Tissues of Fresh Water

Bivalve, Lemellidens corrianus (LEA)M. O. Mulajkar1 and S. P. Zambare2

1Department of Zoology, Walchand College of Arts and Science, Solapur2Department of Zoology, Dr. B.A.M. University, Aurangabad

Abstract: This study done with the observations of biochemical changes in contents of glycogen after chronic exposure and during recovery to lead nitrate (6.81 ppm), (equivalent to the LC50/10 of 96 hours exposure) from testis and digestive gland of control and experimental bivalve, Lamellidens corrianus. During study it was observed that glycogen was significantly decreased in treated bivalves as compeered to control. During recovery glycogen contents recovered and the rate of recovery was faster in caffeine exposed bivalves as compared to those recovered in normal water.Keywords: caffeine, Glycogen, lead, Lamellidens corrianus.

Formulation of Bitter Gourd PickleRanmole P. R., Phulari S. S., Jadhav A. K. and Sonwane C. G.

Dept. of Food Microbiology, Sau K.S.K (Kaku) College of Food Technology, BeedAbstract: Rinse the bitter gourd well. If there are bruises on the skin, then You can slightly peel of that part. No need to peel the entire bitter gourd. Slice the bitter gourd vertically and remove the seed. Chop the bitter gourd into small pieces sprinkle salt on the chopped bitter gourd and mixed well. Keep this bitter gourd salt mixture a side for 120min..After 120min squeeze the salted bitter gourd discard the juice. Rinse once again draining well and keep a side. Mix the bitter gourd chops with the green chillies and lemon. Heat the mustard oil and add mustard seeds with garlic fry till the garlic red and let the oil cool down. Add the fried spices and stir again and let the mixture cool. Just cover the pan with a cotton napkin instead of lid as we don’t want condensation droplet on the lid when completely cooled, pour bitter gourd pickle in a clean sterilized jar. Closed the jar with its lid and keep the bitter gourd pickle in the Refrigerator.We have studied the following physicochemical properties of bitter gourd pickle. The colour, flavour, taste of the pickle is acceptable. The chemical parameters protein is about 1gm\100gm fat is about 2.7\100gm and carbohydrates are 3gm\100gm.

Keywords: Bitter gourd, Fermentation, Preservation, Antidiabetic

ISBN: 978-93-5382-353-5


Page| 69

Variations in Acid Phosphatase (ACP) & Alkaline Phosphatase (ALP) Activities in Liver, Kidney and spleen of Fresh Water Fish

Thynnichthyes Sandkhol Exposed to Copper SulphateP.M. Davne, P.R. Pardeshi and Y. K. Khillare

Department of Zoology, S.B.E.S. College of Science, Aurangabad

Abstract: Fishes are aquatic and poikilothermic animals. Hence, their existence and performance is dominated by the quality of their environment. Pollution of water bodies forces them to acclimatize to various factors thus imposing a considerable amount of stress on their lives. Phosphatase is known to be sensitive to metal exposures and can be used to predict metal toxicity. In this study, freshwater fish Thynnicthyes sandkhol were exposed to different concentrations (0.1, 0.2, 0.5, 1.0 and 2.0 mg/L) of copper sulphate for 96 h, and the group of 2.0 mg/L exposure was then transferred to clean water for different days (2, 8,10 and 14d) to assess recovery profile. Responses of acid phosphatase (ACP) and alkaline phosphatase (ALP) activities from kidney, liver, spleen, to copper sulphate exposure and recovery were investigated. As shown from the results, after a 96-h copper exposure, ACP and ALP activities in different organs/tissues appeared to be different. At the highest copper sulphate concentration (2.0 mg/L), compared with the control, ACP activity decreased significantly in kidney, liver and spleen. ALP activity decreased significantly in kidney, liver, spleen. However, after removing 2.0 mg/L copper sulphate exposure, ACP and ALP activities in different organs/tissues all normalized within 12 days. The observed data suggest that ACP and ALP in spleen of Thynnichthyes sandkhol are most sensitive to copper sulphate stress and might be used as suitable biomarkers for copper contamination in aquatic environment. Keywords: ACP, ALP, copper sulphate exposure, recovery, T. sandkhol

Comparative Study of Glycogen Content in Cestodes Parasites and Their Host Ovis bharal (L.) from Jalna District

Arun Gaware, Meena Nagwanshi, Sunita Borde and Amol ThosarDepartment of Zoology, Dr. B. A. M. University, Aurangabad

Abstract: The present study deals with the study of glycogen content in the cestodes parasite Moniezia, Avetillina, Stilesia and their host intestinal tissue i.e. non-infected and infected intestinal tissue of Oviesbharal(L.). The results obtained in the present study indicate that the amount of glycogen content in thecestodeMonieziais higher than the AvetillinaandStilesia; whereas in host infected intestinal tissue the amount of glycogen content is lower than the non-infected intestinal tissue of the host Oviesbharal (L.).Keywords: Cestodes, Glycogen, Oviesbharal

ISBN: 978-93-5382-353-5


Page| 70

Bioinsecticidal Effect of Natural Saponin from Clitoria Biflora Plant Extract Against, Callosobruchus Chinensis (Coleoptera: Bruchidae)

Bajad P. N.1 and Pardeshi A. B.2

1Dept. of Zoology, Swami Muktanand College, Gangapur2P. G. Dept. of Zoology, Deogiri College, Aurangabad

Abstract: The insecticidal activity of Clitoria biflora L. plant extract against store grain pest, Callosobruchus chinensis was studied. The natural saponin isolated from the plant extracts of Clitoria biflora were tested for their effect on oviposition, adult emergence from grains and mortality of the store grain pest, Callosobruchus chinensis. Observations were made on the number of eggs laid on seeds treated with natural saponin, adult emergence from wheat grains and mortality of adults exposed to treated grains. The number of eggs laid and the adults emerged from grains treated with natural saponin of Clitoria biflora were less than from control seeds. In the present study the natural saponin of extract of Clitoria biflora plant extract were effective as insecticidal property to control store grain pest, Callosobruchus chinensis.

Keywords: Saponin, bioinsecticide, C. chinensis

Histopathology of Indian Major Carps Infected with Myxosporidian Parasites

Somatkar J R1, Dabhade D S2 and Wanjari H V2

1Dept. of Zoology, Late. Pundalikrao Gawali Arts and Science Mahavidyalaya, Shirpur (Jain) Washim

2P.G. and Research Department of Zoology, R. A. Arts, Shri M. K. Commerce and Shri S.R. Rathi Science College, Washim

Abstract: Examination of Indian Major Carp species collected from different localities of Washim region of Maharashtra exhibits the presence of Myxozoan parasites of genus Myxobolus Butschli, 1882 (Myxozoa: Myxosporea: Myxobolidae), Henneguya Thelohan, 1892 and Thelohanellus Kudo, 1933 (Myxozoa: Myxosporea: Myxobolidae). These myxozoan parasites were reported with heavy infection on gill surface and kidney showing yellow or white coloured cysts or plasmodia on gill lamellae. These plasmodia grow in size causing fusion of adjacent secondary gill lamellae as a result large surface of gills is occupied by plasmodia of myxosporidia. This infection results in degeneration of gill lamellae causing reduction of the repiratory surface of fish. The infection of Myxosporidia on kidney results in formation of large cyst causing degeneration and necrosis of renal tubuli and glomeruli.Keywords: Washim, Henneguya, Myxobolus, Thelohanellus

ISBN: 978-93-5382-353-5


Page| 71

Aqueous Herbal Extract of Parthenium Hysterophorus as Eco-Friendly Alternative for Controlling Culex quinquefaciatus

Nikam G. B.1 and Desai A. E.2

Department of Zoology1SSSM Arts, Science and Commerce College, Saikheda, Niphad, Nashik

2MVP Samaj’s KTHM College, Nashik

Abstract: Many diseases are caused by the pathogens which are carried by different species of adult mosquito throughout the world. It is necessary to control the mosquito either in the larval form or in the adult form. Due to increased environmental pollution problem caused by chemical insecticides, now we turned towards the use of herbal extract of many plant parts in the world. The plants comprise a rich source of various phytochemicals that may be widely used instead of chemical insecticides in controlling larval mosquito population. In the present study we are using the aqueous extract of leaves and roots of Parthenium hysterophorus. Parthenium hysterophorus is a noxious weed widely present throughout India. The larvicidal activity was checked for aqueous extract of leaves and roots of it. The aqueous extract of leaves and root of Parthenium hysterophorus is not only used as a larvicides but it also affect on the total protein and carbohydrate content of treated larvae. The protein and carbohydrate content of treated and untreated 4th instar larvae of Culex quinquefaciatus were checked by Lowery et.al. and Anthrone method respectively. The protein and carbohydrate content in the untreated 4th instar larvae of Culex quinquefaciatus was high as compared to treated 4th instar larvae of Culex quinquefaciatus in which the protein and carbohydrate content decreased. The aqueous leaf and root extract of Parthenium hysterophorus shows the repellency. Increased the concentration of leaf and root extract also increased the rate of adult repellency. If the duration increased the adult repellency decreased. The histology of untreated and treated 4th instar larvae of Culex quinquefaciatus was studied which indicates that the aqueous leaf and root extract of Parthenium hysterophorus were altered the histology which is responsible for the death of the 4th instar larvae of Culex quinquefaciatus.The aqueous extract of leaves and root of Parthenium hysterophorus was used to control the population of Culex quinquefaciatus by acting as eco-friendly larvicide, responsible to reduce the protein and carbohydrate content, altered the normal histology and act as a repellent. Keywords: Larvicide, protein content, carbohydrate content, repellency and histological alteration

ISBN: 978-93-5382-353-5


Page| 72

Efficiency of Prepared Organic Compost by Thermophilic Microbes on Vigour Growth of Leafy Vegetables

G. M. Birajdar, V. R. Kumbhar, R. S. Narayankar and U. N. BhaleResearch Laboratory, Dept. of Botany, Arts, Science and Commerce College, Naldurg, Tuljapur,

Osmanaba, Maharashtra

Abstract: Organic farming is a sustainable faming system that produces healthy crops and livestock without damaging the environment. It avoids the use of artificial chemical fertilizers and pesticides, relying instead on developing a healthy and fertile soil and growing a mixture of crops. In this investigation, the prepared(T1-Layered agrowaste;T2-Mixed agrowaste;T3-Mixed fungal culture with agrowaste;T4-Mixed bacterial culture with agrowaste;T5-Mixed Fungi+bacterial culture with agrowaste;T6-Fungal culture with layered agrowaste;T7-Bacterial culture with layered agrowaste;T8-Fungi +Bacterial culture with layered agro waste) compost by pit method and available (FYM-Farm Yard Manuar; VC-Vermicompost; PM-Poultary Manuar) composts were analysed on five important leafy vegetables crops and evaluated the % rate of seed germination and vigour growth. Generally, T3, T4, T5, T6 and T7types of prepared organic compost was observed more significant in %germination and vigour growth and available VC and FYM as well. As compered to available compost, better result was recorded in case of length of stem and root in T4 and T7 treatments. The highest percent (100%) seed germination were found in Rumexacetosa (T5 treatment), Spinaceaoleracea (T7 treatment) and Trigonellafoneum-graecum (T4 treatment) and available VC found 100% seed germination in case of Anethumgraveolens and Coriandrumsativum. In case of other growth parameters prepared type of composts was observed significant result as compared to control and available compost.Keywords: Leafy vegetables; Prepared and available composts, amendments, vigour growth

ISBN: 978-93-5382-353-5


Page| 73

Growth Response and Nutritional Changes Induced by Mycorrhizaeand PGPF in Eggplant (Solanum melongena L.)

U. N. BhaleResearch Laboratory, Dept of Botany, Arts, Science and Commerce College, Naldurg, Tuljapur,

Osmanabad, Maharashtra

Abstract: The soil, AMF or other fungiand host plant are three important factors for interact with each other.The positive effect of native arbuscular mycorrhizal fungi (AMF) are only better visualized when the plants are also grown intheir native soil. Therefore, the present experiment was conducted to assess the effect of AMF and other plant growth promoting fungi (PGPF) for growth response and nutritional changes in brinjal (Solanum melongena). The growth response of plants to AMF and PGPF was studied under natural conditions. The mycorrhizal efficiency index (MEI) and interactive effect of AMF with other fungi (IEF) were calculated on the basis of dry biomass of plants. Biomass of the plantwas effectively enhanced by applying AMF alone or mixed with others. The mycorrhizal efficiency index showed maximum biomass benefit due to the application of AMF + Trichoderma harzianumand AMF+ T.viride followed by AMF + Aspergillus niger. The nutrient uptake was enhanced during thecombined application of AMF along with PGPF. However, the uptake efficiency was most reverse with respect to control (non-mycorrhizal) plant. Assay on the mycorrhizal nutrient efficiency (MNE) in brinjal indicated that combined application of AMF with T. virideand other PGPF enhanced the uptake of P, K, S, Mn and B than non-mycorrhizal control. Besides it also increased the uptake of Zn and N. Comparative evaluation between AMF and other indicated that the interactive nutrient efficiency (INE)in relation to the uptake of P, K, Ca, Mg, S, Mn and B was the maximum in AMF + T. viride treated plants rather than the mycorrhizal control.Keywords: Solanum melongena, biomass, Micro and Macronutrients, AMF, PGPF.

ISBN: 978-93-5382-353-5


Page| 74

Isolation and Characterization of Rebellious Bacteria Streptomyces Spp. and Its Characterization Using 16S rRNA Sequence

Rupali A. Padol and Kshama G. DeshpandeDept. of Microbial and Environmental biotechnology, MGM College of agricultural

biotechnology, Gandheli, Aurangabad

Abstract: Strptomyces griseus is an important antibiotic producing bacteria. It can be used as an alternative to the chemical fungicide, bactericide and it produces armed conflict around the plant against various pathogens. Present work is on isolation of Streptomyces, its characterization and molecular identification. Streptomyces bacteria were isolated from soil sample of MGM Hill’s Gandheli. This soil contains fewer amounts of nutrients leading to fewer yields. Streptomyces griseus produces novel secondary metabolites and decompose stubbles of previous crop, plant waste material, it can be used to increase soil fertility and increase yield. Biochemical and morphological characterization of isolated spp. was carried out and it was confirmed by using partial 16s rRNA sequencing.For molecular identification, molecular method of partial 16S rRNA gene sequencing was used to determine the genetic diversity of multifunctional bacteria Strptomyces Spp. DNA was isolated from the fresh cultured cell and it was amplified with 16S rRNA gene. Sequence analysis of sequence obtained from ABI 3130 Genetic Analyzer was subjected for BLAST search in GeneBank. BLAST result showed that the test organism was similar to Strptomyces griseus with 99% similarity and E value 0.0. Finally it was found that the isolated bacterium was Strptomyces griseus and it will be useful to increase yield and quality of crop and plant protection by avoiding the use of chemical fertilizers.Keywords: Streptomyces griseus, 16S rRNA sequencing, Rebellious bacteria, Antibacterial, Antifungal

ISBN: 978-93-5382-353-5


Page| 75

Identification and Characterization of Active Sulphur Oxidizing Bacteria Thiobacillus thioxidans by 16S rRNA Sequence Analysis

Rushikesh Ravindra Tahakik and Kshama DeshpandeDept. of Microbial and Environmental Biotechnology, MGMcollege of agriculture biotechnology,

Gandheli, Aurangabad

Abstract: This Experiment aims at developing Soil friendly sulphur biofertilizer and their molecular analysis. This was conducted to isolate active sulphur oxidizing bacteria from Soybean rhizosphere at MGM Hill’s Gandheli and 16S rRNA gene of these species was sequenced and identified.During Study of the bacterial decomposition of thiosulphate and thiocyanate in agricultural soil of MGM Hill’s Gandheli, it became necessary to identify the organisms responsible. These were thiobacilli, and one in particular, appeared identical with Thiobacillus thiooxidans by various biochemical analyses. To confirm this, its 16S rRNA gene Sequence was compared with various Thiobacilli in GeneBank by using nBLAST tool. As a result of this comparison it was investigated that test organism was similar to acidothiobacillus thioxidans, Thiobacillus denitrificans with 90% similarity and E value 0.0. Finally it was found that identified organism Acidithiobacillus spp. is capable of reducing sulphur and calcium salts contaminants in the soil.Trials were carried out by using many subcultured samples of isolated Thiobacillus on various types of soils for its sulphate and calcium solubilization.Keywords: Thiobacillus, Biofertilizer, Rhizosphere, 16S rRNA, BLAST

ISBN: 978-93-5382-353-5


Page| 76

A Report on Occurrence and Incidences of Insect Pests Associated with Mulberry Gardens of Shevgaon Taluka

Popat P. Pathare, Chandrashekhar J. Hiware and Anil N. KhadeDepartment of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad

Abstract: Mulberry plant (Morus alba) is important sole food for mulberry silkworm (Bombyx mori L.). Mulberry plants leaves play exclusive role in silk production but many insect pests are attacking on mulberry plant and they adversely effects on mulberry plant which results on deterioration of quality and production of mulberry plant leaves. The results show that, the different insect pests are damaging to Mulberry crop in study area which causes heavy economical loss of sericulturists or farmers. Insect pests were found in mulberry gardens of study area are Bihar hairy caterpillar, Wingless grasshopper, Read hairy caterpillar, Lemon butterfly and Thrips etc. The present study is carried out to know occurrence and incidences of mulberry insect pests &nature of damage, symptoms of insect pests of mulberry gardens from various sites of Shevgaon taluka of Ahmednagar district of Maharashtra state. The present study was conducted in March-2015 to February 2017.The results of the fields study revealed that the incidence of Bihar hairy caterpillar was started from month of August to January in all the fields of mulberry gardens, it’s indicate the Bihar hairy caterpillar is major insect pests of mulberry plant of study area.Keywords: Bombyx mori L., Incidence, Insect pests, Mulberry, Silkworm

ISBN: 978-93-5382-353-5


Page| 77

Effect of Selected Medicinal Plants Leaf Biomass against Selected Seed Borne Fungi of Methi

D. M. SurvaseDept. of Botany, Karmveer Ramraoji Aher Arts, Science & Comm. College, Deola, Nashik

Abstract: Fungi are most destructive pathogen for the crop plants. After harvesting crop, many microorganisms may be growing on the stored seeds. These fungal pathogen spores adhere to seeds or internally growing. Such affected seeds are not used for sowing or food purposes.In the present study seeds of common vegetables have been selected for this study. Isolation of many fungi has been carried out by the method described by ISTA, 1966. Many fungi isolated from vegetables seeds, out of which four very common fungi have been selected and brought into pure culture. Out of these fungi Aspergillus niger and Fusarium oxysporum were very common than the Curvularia lunata and Drechslera longirostrata. Effect of very common ten medicinal plants leaf extract have been tested against spore germination, dry mycelial weight and sporulation of these selected four fungi have been carried out. It is clear from the study that Solanum xanthocarpum, Semecarpus anacardium, Dioscorea bulbifera and Aegle marmelos leaf extract were found more inhibitory for spore germination, growth and sporulation of seed borne fungi. Similar studies have been carried out in Okra by Mashooda Begam and Lokesh (2008). Similar results have been reported by Prabha et al (2003), Ashish et al (2008), Muzumdar et al (2004).Keywords: Medicinal plants, Spore germination, Dry mycelial weight, Vegetables seeds

Blue Green Algal Flora of Lonar Crater LakeRafiullah M. Khan1 and Milind J. Jadhav2

1Department of Botany, Kohinoor College, Khuldabad, Aurangabad2Department of Botany, Sir Sayyed College, Roshan Gate, Aurangabad

Abstract: Lonar lake is natural lake, situated in Buldhana district of Maharashtra. The exact geographical location of Lonar lake is at 19o 55’ 45’’ North Longitude and 76o 34’ 00’’ East Latitude. The water of lake is saline with a high pH. In present study algal samples from selected sites of Lonar lake were collected at monthly intervals, October 2015 to September 2016. Acid washed collection bottles were used for the collection for algal samples. In present study 51 species under 20 genera have been identified and recorded. Algal genera such as Arthropira, Aphanothece, Spirulina, Oscillatoria, Phormidium, Plectonema,Microcystis and Gloeothece were dominant. Blue green algal flora of Lonar lake is rich and it is found in diverse form. The lake water was observed green in colour due to abundance of blue green algae in lake water.

Keywords: Blue green algae, Lonar lake

ISBN: 978-93-5382-353-5


Page| 78

Evaluation of Anti-diabetic and Anti-oxidant Activities of Wild Edible Vegetables: Clerodendrum serratum (L.) Moon and Basella alba L.

Smita A. Sonawale and Anjali Abhay KulkarniDepartment of Botany, Savitribai Phule Pune University, Pune

Abstract: In the last few years, numbers of diabetic cases have increased world-wide and especially in India. Currently used oral hypoglycaemic agents like sulfonylureas, metformin, trigolitazone etc. have a number of adverse side effects, such as liver problems, lactic acidosis, diarrhoea etc. Thus there is demand for new compounds for treatment of diabetes and related complications. Plants have always provided us with many therapeutically active compounds with minimum side effects. These belong to class of phenolics, alkaloids, flavonoids, terpenoids, coumarins and glucosides.

We examined two wild edible plants from Konkan region for their therapeutic effects against diabetes and related disorders. Leaves of C. serratum (L.) Moon and B. alba (L.) were extracted in different solvents and were analyzed for their anti-diabetic and anti-oxidant activities. Preliminary phytochemical tests showed presence of alkaloids, flavonoids, steroids, terpenes, phenols, tannins, glycosides and quinines. For anti-diabetic evaluation, in vitro α- amylase inhibitory assay was carried out with crude extracts of both the plants in different solvents. Methanol extracts of both the plants showed strong α- amylase inhibitory activity in comparison with Acarbose (the current therapeutic drug being used). These extracts were fractionated with column chromatography. Each individual fraction was analysed and compared with a number of standard compounds by TLC. We observed that the extracts contained β- sitosterol, Quercetin, and Kaempherol. Each of the TLC bands matching with the standards showed strong α- amylase inhibitory activity. For anti-oxidant study, DPPH and H2O2 assays were performed. Again methanol extracts of both the plants showed good antioxidant activity with respect to IC50 value of standard reference compound: Ascorbic Acid. β- sitosterol, Quercetin, and Kaempherol were the active free radical scavenging compounds implicated.Thus we can conclude that α- amylase inhibitory activity and anti-oxidant activity of leaves of C. serratum (L.) Moon and B. alba (L.) is due to presence of β- sitosterol, Quercetin, and Kaempherol. This validates the use of leaves of these wild medicinal vegetables in our diet.Keywords: Clerodendrum serratum, Basella alba, anti-diabetic, anti-oxidant, phytochemistry

ISBN: 978-93-5382-353-5


Page| 79

Efficacy of Seed Extracts of Semicarpus anacardium Against Indian White Termite, Odontotermes obesus Under Laboratory Conditions

(Isoptera: Termitidae)Nagare G. J., Gadewad M. G. and Pardeshi A. B.

P. G. Department of Zoology, Deogiri College Aurangabad

Abstract: Termiticidal effect of petroleum ether and hexane seed extract of Semecarpus anacardium were studied against Odontotermes obesus. The plant seeds were dried, powdered and extracted with petroleum ether and hexane solvent in soxhlet apparatus for 24 hrs. The 10 termites were exposed to various concentrations (2.0, 4.0, 6.0, 8.0 and 10 mg/ml) of petroleum ether and hexane solvent seed extract of Semecarpus anacardium and percent mortality were recorded after 24 hrs.The termiticidal activity of seed extract of Semecarpus anacardium were (LD 10= 2.480 mg/ml, LD 50= 6.823 mg/ml) in petroleum ether and (LD10= 2.494 mg/ml, LD 50= 7.243 mg/ml) in hexane solvent respectively. Results revealed that the mortality was increased with increasing in concentration of the seed extracts.The petroleum ether solvent extract of Semecarpus anacardium showed higher termiticidal property against Odontotermes obesus. Statistical variance, 95% confidence limits and regression equations are presented.Keywords: Termiticide, Odontotermes obesus, Semecarpus anacardium.

Influence of Phytoecdysteroids Isolated from Coix Aquatica on Cocoon Characteristics of Silkworm Bombyx mori L.Barge S. B.1, Pardeshi A. B.1, Patil R. P.2 and Reddy Y. R.1

1P. G. Department of Zoology, Deogiri College, Aurangabad2P. G. Dept. of Botany, Deogiri College, Aurangabad

Abstract: Influence of phytoecdysteroids isolated from the plant, Coix aquatica on the cocoon characteristics of silkworm, Bombyx mori was studied. The experiments were performed by 0.5, 1.0, 1.5, 2.0 and 2.5% concentration of phytoecdysteroid obtained from methanol solvent leaf extract of Coix aquatica and administered to 4th and 5th in stars of silkworm with mulberry. The cocoon characteristics were influenced by various concentration of plant extract. The intensity of influence was depending on the time and dose exposure. The phytoecdysteroid at 2.0% concentration resulted increased cocoon weight. The average silk filament length, fibroin and sericin and denier were also increased with this supplementation of phytoecdysteroid over the control. In the present study the phytoecdysteroid of Coix aquatica have growth promoting effect and improved the cocoon characteristics of silk in Bombyx mori. Keyword: Plant extract, Bombyx mori, Fibroin, Sericin

ISBN: 978-93-5382-353-5


Page| 80

Study on the Bioassessment Protocol and Metrics by Using Physical Habitat Condition and Fish Assemblage of Central Indian River Sip

1Parul Gurjar*, 2Vipin Vyas, 3Rumeet Kaur Raina, and 1Kuldeep Lakhera1 Department of Environment Science & Limnology, Barkatullah University, Bhopal

2Department of Bioscience, Barkatullah University, Bhopal3 Department of Zoology & Applied Aquaculture, Barkatullah University, Bhopal

Abstract: The habitat study in terms of species ecology is as important as water quality because habitat defines the type of species inhabiting there. Habitat is considered as one of the most important tools for assessment of the fish survival as it defines their health and abundance. It is important for individual species as well as for the assemblage of fishes. The objective of this study is to assess the impairment of river ecosystem by the anthropogenic activities. For evaluating the aquatic health physical habitat condition and fish status were taken as a tool.Sip River is a Tributary of River Narmada which is a Central Indian River, joining Narmada at right bank just upstream of Indira Sagar reservoir. Seven sites were selected from the 74km river. In the present study, the physical habitat condition was assessed by using Rapid Bioassessment Protocol (RBP’s) in terms of visual assessment. The fish assemblage was assessed by using an attribute (number of tolerant/intolerant fishes) from the Index of Biotic Integrity (IBI) developed for Indian rivers.In the total assemblage, it is found that the intolerant species were abundant on the sites where the habitat is less destructive or is untouched by human perturbance. Alternatively, the stations where human interference is more, the tolerant fishes were found significant and diverse. The tolerant species signifies the pollution level in the aquatic ecosystem and has been found only on two sites which are facing the most anthropogenic disturbance among all sites. The change in land use pattern with respect to the increasing developmental activities near the banks is affecting the habitat of the organisms residing in the aquatic ecosystem.Keywords: Biodiversity, Ecology, River, Aquatic Ecosystem, Habitat

ISBN: 978-93-5382-353-5


Page| 81

Analysis of Macroinvertebrate Functional Feeding Groups of River Sip,in Central India

Rumeet Kaur Raina1, Vipin Vyas2, Abha Swarup3 and Parul Gurjar4

1Department of Zoology and Applied Aquaculture, Barkatullah University, Bhopal2Department of Bioscience, Barkatullah University, Bhopal

3MP council of Science and Technology, Bhopal4Department of Environment Science, Barkatullah University, Bhopal

Abstract: Macroinvertebrates play important roles in many ecological processes viz. nutrient cycling, primary production, decomposition and material translocation. The functional feeding group(FFG) approach is an attempt to classify organisms according to their role in the processing of organic matter; however this approach eliminates the need for a detailed taxonomic description and also constitutes the important tool for biomonitoring. The objective of this study was to analyze the health of River Sip using macroinvertebrate functional feeding groups. A total of 61 taxa were recorded during the study period. The identified macroinvertebrates were classified as one of the following functional feeding groups: Shredders, Gathering collectors, Filtering collectors, Scrapers and Predators. The highest percentage was contributed by predators. The notable characteristics of the river were observed which represent low percentage of the shredder functional feeding group in the community.Keywords: Functional feeding groups (FFG), Sip River, Narmada, Aquatic Macroinvertebrates, Shredders

Comparative Extraction of Tephrosia purpurea And Evaluation of Its Antioxidant Activity

Kanchan Nikam, Nutan Kendre, Vikas Bhise, Pravin Wakte

Abstract: Tephrosia purpurea is a wild perennial plant belonging to family Fabaceae. It has been used as a folk medicine for the treatment of a number of diseases. In Ayurveda it is described as ‘Sarva warnavishapak’ means it has a property to heal all types of wounds. The plant has been claimed to cure diseases of the kidney, liver spleen, heart, and blood. The whole plant of Tephrosia purpurea was subjected to Soxhlet assisted extraction (SAE) and Ultrasound assisted extraction (UAE) using ethanol as a solvent. Both the extracts were subjected to antioxidant activity using DPPH radical scavenging assay and Ferric (Fe3+) Reducing Power Assay. The extract obtained from UAE shows highest extraction yield, DPPH scavenging activity and ferric reducing power when compared with the standard Ascorbic acid. The proposed research work showed the UAE can be a method of extraction for T. purpurea. The antioxidant activity done by DPPH radical scavenging assay and Ferric (Fe3+) Reducing Power Assay may be useful for further research work of T. purpurea.Keywords: Tephrosia purpurea, Soxhlet Assisted Extraction, Ultrasound Assisted Extraction

ISBN: 978-93-5382-353-5


Page| 82

Molecular Characterization of Pigmented Bacteria from Alkaline WaterGeetanjali R.K1,2, S.V. Hiremath2 and M.B.Hiremath1

1Department of Studies inBiotechnology and Microbiology,Karnatak University, Dharwad2Department of Biotechnology and Microbiology, P.C.Jabin science College Hubballi

Abstract: Microbes are the attractive option for the synthesis of pigments due to their easy cultivation, cost effective production and wide applications in various industries like food, pharmacy, textile, cosmetics, etc. Moreover the microbes from the marine source have unique features as they are adapted to extreme physical parameters as pH, temperature and salinity. The present study aims for the isolation and molecular characterization of pigment producing bacteria from the marine water sample, and to check the biological activity of the pigment. Five novel pigment producing bacteria were isolated, of these; onebacterial isolate producing prominent extracellular pigment was selected for the further study. Optimum physical parameters observed for maximum pigment production were pH-10, temperature 37°Cwith agitation for 90 hours of incubation. The antibacterial activity of the crude pigment extract was assayed for five common human pathogens and highest activity was against Escherichia coli at a concentration of 100µg/ml. Further the crude pigment extract had anticancer activity against Human cervical cancer cell line (HeLa). Thus present study demonstrates the biomedical application of the pigments harnessed from the bacteria. These results highlight the fact that, unlike synthetic pigments, the pigments derived from bacteria, not only add colour but also demonstrate beneficial biological activities.Keywords: Bacteria; Pigments; HeLa; Biological activity

Endophytic Fungi Associated with Cajanus cajan, Linn. Produces Novel Bioactive Compound Cajaninstilbene Acid (CSA)

Shinde S. Y., Deshmukh P. D. and Sanap S. B.Department of Botany, Late Shankarrao Gutte Gramin, Arts, Commerce and Science College,

Dharmapuri, Parli (V.), BeedAbstract: Plant kingdam is a rich source of structural biodiversity and offers a variety of natural products. Plants have been utilized to produce various types of medicines for thousands of years. Fungal endophytes from plants are well known for the production of bioactive metabolites that have wide prospects for use in industry and medicine. Endopytes are ubiquitous in nature found in vitually all plant species studied to date. The Present study aims isolation and screening of novel endophytic fungi producing Cajaninstilbene acid (CSA) from Cajanus cajan, Linn. Millsp. CSA has prominent pharmacological activities. Total 50 endophytic mycoflora were grouped into 6 genera on the basis of morphological characteristics. CSA producing fungi were identified as Fusarium solani, Fusarium oxysporum, Fusarium proliferatum and Fusarium moniliformae respectively.Keywords: Cajanus cajan, Linn., Endophytic mycoflora, Bioactive compound, Cajaninstilbene acid (CSA), Isolation, Screening

ISBN: 978-93-5382-353-5


Page| 83

Studies on Occurrences of Hemipteran Insect Pests on Mulberry (Morus alba L.) from Aurangabad

Avhad Sunil B.1 and Hiware Chandrashekhar J.2

1Department of Zoology, Annasaheb Vartak college of Arts, Kedarnath Malhotra College of Commerce, E.S. Andrades College of Science, Vasai road, Palghar

2Department of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad

Abstract: Mulberry (Morus spp.) leaf is the only natural food for the silkworm, Bombyx mori L. It is a perennial, evergreen, luxuriant crop cultivated in all types of soils, both under rainfed and irrigated conditions. So far, over 300 insect and non-insect species of pests are known to infest mulberry in varying intensities during different stages of the crop and seasons. The present investigation shows taxonomical study on 9 species Hemipteran insect pests namely Aonidiella qurantii, Dysdercus singulatus, Empoasca flavescens, Maconellicoccus hirsutus, Nezara viridula L., Oxyrachis tarandus and Tessaratoma javanica, Erthesina acuminata Dallas, 1851 and Hotea curculionoides of mulberry garden from various sites in Aurangabad district, Maharashtra, India. In present work during study period some important Hemipteran pests of mulberry are described with respect to distribution, occurrence and type of damage and symptoms.

Keyword: Taxonomy, Distribution, Damage and symptoms

GC-MS Analysis of Alcoholic Extract of Hemidesmus indicus Roots and Its Antibacterial Studies

Nutan Kendre, Madhuri Deshmukh, Pravin Wakte

Abstract: Hemidesmus indicus also known as “Anantmuli” belonging to family Asclepiadaceae is commonly used herb from ancient time. It has been traditionally used to cure various ailments like leucoderma, asthma, syphilis, dyspepsia, dysentery, gonorrhea, diabetes, stomach ulcer, spennatorrhoea etc. The aim of present research work was to carry out antibacterial studies and GC-MS analysis of alcoholic extract of Hemidesmus indicus roots. The preliminary phytochemical analysis reveals the presence of various phytoconstituents like alkaloids, polyphenols, saponins, flavanoids, carbohydrates, reducing sugar etc. The extract shows good inhibition zone against Pseudomonas aeruginosa and Escherchia coli compared with standard antibiotic streptomycin. GC-MS analysis revealed the presence of various constituents which may responsible for its antibacterial activity. The current research work indicates the importance of Hemidesmus indicus as an antibacterial drug. The results obtained from proposed research work may be useful for identification and isolation of Phytoconstituents responsible for antibacterial activity.Keyword: Hemidesmus indicus, GC-MS, antibacterial studies, Pseudomonas aeruginosa, Escherchia coli

ISBN: 978-93-5382-353-5


Page| 84

Alkylating Agent Induced Hepatic Oxidative Stress and Histopathological Lesions Cured by Royal Jelly

Syeda Hina Kausar and V. R. MoreDepartment of zoology Government College of Arts and Science, Aurangabad

Abstract: The study was conducted to evaluate the effect of royal jelly on liver against alkylating chemotherapeutic agent Cyclophosphamide (CPA) induced alterations. CPA, generally is used in a treatment of varieties of cancers such as leukemia, lymphoma, breast, lung, prostrate, ovary. Besides this, the side effects of CPA are also well known such as Bone marrow toxicity with opportunistic infections, hemorrhagic cystitis, temporary infertility, nausea, vomiting and hair loss etc. Royal jelly (RJ) is an antioxidant agent produced by the hypo pharyngeal glands of worker honey bees. It has cyto-protective properties against CPA-induced hepatic toxicity, pathological lesions to liver and oxidative stress.24 healthy mice were divided into 6 group (G1-G6) and treated as: G1: 10ml/kgNS,G2:100mg/kgRJ,G3:50mg/kgCPA,G4:RJ(100mg/kg)+CPA(50mg/kg),G5:RJ(250mg/kg)+CPA(50mg/kg),G6:RJ(500mg/kg)+CPA(50mg/kg) for three months. After experimental periods they were anesthetized and sacrifice there liver were excised and used for the biochemical and histopathological estimation.In the liver RJ scavenges the free radicals to prevent the toxic effects of CPA as shown by the biochemical investigation and might be responsible for significant protection against cyclophosphamide-induced hepatic damages.During chemotherapy, as a natural product RJ might be used in a regular basis for the reduction of hepatic damages.Keywords: Cyclophosphamide, Royal jelly, free radicals, hepatic damages

ISBN: 978-93-5382-353-5


Page| 85

Formulation and Evaluation of Egg Shell Bhasma LozengesChandrawanshi Mayuri J., Pattewar Shraddha G., Sakhare Ram S. and Nagoba Shivraj N.

Channabasweshwar Pharmacy College (Degree), Latur

Abstract: Calcium is essential mineral which helps build and maintain bone, teeth. It also plays important role in number of body processes like blood clotting, blood vessel and muscle contraction. It also regulates enzyme and hormone secretion and also in central nervous system function. When the amount of calcium in the bloodstream is low, calcium stored in bones is broken down and used in bloodstream which leads to calcium deficiency diseases like cardiovascular diseases, kidney diseases, rickets, and calcification of soft tissues, memory loss, brittle bone, hallucination, depression, eye diseases and spinal fracture. Calcium deficiency makes the bone weak, raising the risk of fracture and increasing the risk of osteoporosis, the condition in which bone mineral density goes on decreasing and makes the bones fragile which lead to fracture. This condition is treated by using hormonal and estrogens replacement therapy, biphosphonates, estrogens agonist and antagonist, using anti-inflammatory agent, calcium supplement etc. but all these shows severe side effects and has high cost. The most effective, ideal, novel and beneficial treatment for osteoporosis with low cost and negligible side effects is by using eggshell bhasma as calcium source. Eggshell is rich source of calcium up to 95%, has 60% more bioavailability than purified calcium carbonate, and increases bone mineral density in osteoporosis patient.Lozenges are the flavoured medicated dosage forms proposed to be sucked and held in the mouth or pharynx containing one or more medicament usually in sweetened base. Lozenges are already broken down before entering our stomach making them easier for our body to absorb. Egg shell bhasma lozenges are prepared by using sugar, fillers, lubricants, binders, polymers, colouring and flavouring agents. Lozenges are evaluated by hardness, weight variation, thickness, drug content, in-vitro drug release study, FTIR study and stability study etc.

Keywords: Egg shell bhasma, lozenges, calcium source, bone mineral density etc.

ISBN: 978-93-5382-353-5


Page| 86

Evaluation of Anti-oxidant and Anti-inflammatory Activity of Hydro-alcoholic Root Extract of Salacia fruticosa.Wall

Vishnu R Varma, S R Suja and R PrakashkumarEthnomedicine & Ethnopharmacology Division, Jawaharlal Nehru Tropical Botanic Garden &

Research Institute, Palode Thiruvanthapuram

Abstract: From the ancient times medicinal plants are used to treat many diseases like diabetes, malaria, chronic fever, cold, cough, diarrhea, arthritis, etc. Oxidative Stress and inflammation are the major cause behind the pathogenesis of most of the diseases including Cancer, Cardiovascular and Neuro degenerative diseases. Salacia –a member of Celastraceae family is one of the medicinal plants extensively used to treat numerous ailments viz. hypoglycemic, inflammation, hypolipidomic, etc.The objective of the present study was to evaluate the anti-oxidant and anti-inflammatory activity of 50% Hydro-alcoholic root extract of Salacia fruticosa. Wall (Ponkoranti). The in vitro antioxidant property was assessed by evaluating the DPPH free radical scavenging activity, total reducingpower, total antioxidant property and Nitric Oxide Scavenging activity of the samples and followed by a quantitative phytochemical estimation of total Phenolic content, Flavonoid content and estimation of total condensed Tannins.The anti-inflammatory activity was evaluated using Carrageenan and Formalin-induced paw oedema models in Wistar albino rats.The 50% Hydro-alcoholic extract of S.fruticosa shows significant antioxidant activity in DPPH free radical scavenging activity, total reducingpower, total antioxidant property and Nitric Oxide Scavenging activity and also shown significant presence of phytochemical constituents such as phenolics, flavonoids and tannins which are expressed in gram equivalence of the standard Gallic acid, Rutin and Catechin respectively. The anti-inflammatory activity was found to be dose dependent in Carrageenan-induced paw oedema model. The 50% Hydroalcoholic extract has shown significant inhibition of paw oedema, at the dose of 150mg/kg with respect to the standard Indomethacin. The maximum percentage inhibition in paw oedema was observed at the doses of 150mg/kg with respect to the standard Aspirin.The results of the present study demonstrate that 50% Hydroalcoholic extract of S. fruticosa Wall root exhibits significant anti-oxidant as well as anti-inflammatory properties which is significant for the treatment of several diseases including, Cancer, Cardiovascular diseases and Neurodegenerative diseases.

ISBN: 978-93-5382-353-5


Page| 87

Prevention of Biofilm Formation in Urinary Catheter by Antibiofilm Agents

Dhale D. A.1 and Anuja P. Maitreya2

1Post Graduate Department of Botany, SSVPS’s, L.K.Dr.P.R.Ghogrey Science College, Dhule2Post Graduate Department of Microbiology, RashtrasantTukadojiMaharaj Nagpur University,

Nagpur

Abstract: The present study emphasize with investigation of microbial contamination in indwelling urinary catheters. Biofilm forming ability of the isolates were determined by tissue culture plate method. Prevention of biofilm formation in the urinary catheter by Pseudomonas aeruginosawas also determined by coating the catheter with some enzymes, gentamycin, EDTA, DNase enzyme,Antimicrobial drugs-Ceftazidimine and Ceftriaxone, some enzymes,gentamycin, EDTA and plant extract (Azadirachtaindica).Out of 20Urinary catheter sample,8 samples shows bacterial growth.It was found that 40% Urinary catheter gets contaminated during the course of catheterisation.Of the total 8 isolates biofilm formation was seen in 100% Pseudomonasspecies and Klebsiellapneumoniae and 50% S. aureus. Ampicillin andVancomycin (100%) were highly resistance against Klebsiellasp.,Pseudomonassp.& S.aureus.While Ciprofloxacin and Penicillin are sensitive against S.aureus. From the result obtained in different concentration of antibiofilm agent,we can interpret that biofilm production was completely prevented in 3% Ciprofloxacin, 3% EDTA,5% Tobramycin,5% Lysozyme and 5% α-amylase concentration. The 500µl Azadirachtaindicaplant extract was also capable of dispersing the biofilm formation inPseudomonassp. ,S.aureusand Klebsiellapneumoniae.Keywords: Azadirachtaindica, Biofilm, Enzymes, EDTA, Urinary catheter

ISBN: 978-93-5382-353-5


Page| 88

Cyborg Botany: Plant-Robot HybridsAnjali S. Naik and Asmita B. DasputeSBES College of Science, Aurangabad

Abstract: It is a well-known fact that plants respond to environment in a number of ways. Response of plants to light and water and conversion of these stimuli to manufacture food is a basic knowledge. Scientists are now trying to harness the sensory capabilities of plants like their response to touch, sound, hearing and smell, and convert it to mechanical or electronic display. These plants are called cyborg plants.Rather than using traditional sensors, scientists are experimenting with plants’ innate sensing abilities to create interfaces e.g. sensing (signals inside plants), and response (plant movement, color change, leaves opening/closing, growth etc.), Plants as interfaces as separate but within nature itself. “Plants can sense and they can display the detectable response” is the theme of this review paper. MIT Media Lab, has created a robotic plant that acts like a light sensor. This plant-robot cyborg uses electrodes, light detectors, and wheels to save a plant from perishing.The plant’s leaves can sense the small electrical signals within the leaves that respond to the presence of light. When the plant detects that there’s light nearby, these signals trigger the wheels of its robotic planter, which autonomously moves toward the light source.Cyborg plants in future may replace such sensor systems which are presently used in the electronic form to monitor crops. These sensors monitor the air or ground for water, nutrients and other things that matter to plant health. Any pollutants in the soil or groundwater will get pulled in the roots. Sensors within a plant’s roots or xylem should then be able to scout for particular chemicals.Scientists have developed plants working as sensors that can even detecta bomb, and produce signals by emission of infrared light. Once the camera detects the infrared light, the computer connected to it emails out a warning. Thus, an intensive research is being carried out to use plants as green sensors or cybernetics.Keywords: Cyborg, Cybernetics, Biosensors

ISBN: 978-93-5382-353-5


Page| 89

Study of Chemical Composition of Alstonia scholaris Leaf Extract Using GC-MS Technique with Insight on Larvicidal Activity against Aedes

aegyptiMamta Goyal and Laxmikant Shinde

Applied Parasitology Research Lab., Department of Zoology, JES College, Jalna, Maharashtra

Abstract: Nature has bestowed us with its tremendous biodiversity in plant species. These plants serve as our best companion either as edible or medicinal attributes. The phytochemicals from various parts of plants contributes protection to plant and also medicinal significance to mankind. In present study, we aimed to search for qualitative and quantitative estimation of various phytochemicals in leaf extract of Alstonia scholaris (L.)R. Br. by GC-MS analysis. The extract was also tested against third instar larvae of Aedes aegypti to evaluate larvicidal potential. Aedes aegypti act as a vector to spread many deadly diseases like Dengue. Chikunguniya, Zika virus.GC-MS analysis of hexane leaf extract of Alstonia scholaris revealed the presence of 32 identified components .The majority of bioactive components found to be rich in hydrocarbon group and consists of pentane, 2-methyl (31.30 %); pentane 3- methyl (31.09 %); methyl cyclopentane (14.21 %); cyclohexane (20.70 %). In addition to this, various other bioactive phytochemicals comprises of squalene (1.63 %); fatty acids (0. 45 %); phytol (0.07%). These phytochemicals weather present in more or trace amount contributes to insecticidal activity. The LC50 value of extract tested against third instar Aedes aegypti was 199.1 ppm.The findings of present study prove to be beneficial in control of dengue vector Aedes aegypti with eco-friendly botanical origin. A better substitute for synthetic insecticides to control mosquito. Key words: Aedes aegypti, GC-MS analysis, LC50 and LC 90,

ISBN: 978-93-5382-353-5


Page| 90

Bioinsecticidal Effect of Endophytic Fungal Extract of Azadirachta indica against Callosobruchus chinensis Linn. (Coleoptera: Bruchidae)

A. B. Pardeshi1, R. B. Patil2 and P. N. Jadhav3

1Department of Zoology, Deogiri College, Aurangabad 2Department of Zoology, ACS College, Lasalgaon 3Department of Zoology, ACS College Nandgaon

Abstract: Endophytic fungi are microorganisms that live inside the plant without causing any over negative effect on plant tissues; rather they protect the host plant from pests and diseases. The insecticidal activity of endophytic fungal extract of leaf and seed of Azadirachta indica against pulse beetle, Callosobruchus chinensis, were studied. Endophytic fungi were isolated from the leaf and seed of Azadirachta indica. The various concentrations of methanol and ethyl acetate crude endophytic fungal extracts were tested against Callosobruchus chinensis. The percent mortality was recorded after 96h. The insecticidal activity of the endophytic fungi isolated from leaf of Azadirachta indica were (LD =19.81 mg/kg, LD =84.63 mg/kg) in methanol and (LD =39.17 mg/kg, LD =110.3 mg/kg) in ethyl acetate 10 50 10 50 respectively. The fungi isolated from seed of Azadirachta indica were (LD =12.25 mg/kg, LD =39.80 mg/kg) in methanol and (LD =28.97 mg/kg, LD =60.20 mg/kg) in ethyl acetate respectively. The mortality increases with increase in 10 50 concentration of endophytic fungi. The methanol solvent extract showed more insecticidal property against Callosobruchus chinensis due to the secondary metabolites of endophytic fungi. Statistical variance, 95% confidence limits and regression equations are presented.Keywords: Callosobruchus chinensis, Endophytic fungi, Mortality, Bioinsecticide, Azadirachta indica

ISBN: 978-93-5382-353-5


Page| 91

Studies of Enzymatic Activity of Charcoal Rot of Sorghum Caused by Macrophominaphaseolina

M. T. Gavali, G. M. Birajdar and U. N. BhaleResearch Laboratory, Department of Botany, Arts, Science and Commerce College, Naldurg,

Tuljapur, Osmanabad

Abstract: Present paper deals on enzymatic activity of charcoal rot of sorghum caused by MacrophominaphaseolinaProduction of enzymatic activity e.g., Cellulase, Amylase, Lipase, Pectinase, Xylanase, Catalase, Peroxidase and Esterase of M. phaseolina incitant to charcoal rot of sorghum was determined. Extracellular production of hydrolytic enzymes was the unique feature of M. phaseolina that was the main reason of their infectious nature. All these enzymes were detected and results were recorded with mancozeb sensitive and resistant isolates. Macrophominaphaseolina was observed the enzymatic activity such as cellulose activity zone on sensitive (22mm) and resistant (30mm), amylase enzyme, sensitive (33mm) and resistant (37mm), lipase enzyme activity sensitive (20mm) and resistant (26mm). Loss of viscosity of the pathogen M. phaseolina, sensitive (75.83%) and resistant (86.78%) at (30min) showed maximum in pectolytic enzyme. Xylanase enzyme activity zone was foundsensitive (27 mm) and resistant (38mm). Catalase and peroxidase was found minimum and medium response Esterase enzyme activity was found negative response.Keywords: Charcoal rot, sorghum,Macrophominaphaseolina, enzymes

Medicinal Values of Some Indian SpicesPreeti Kulshrestha

Dept. of Botany, Govt. K.R.G. P.G. Autonomous College, Gwalior

Abstract: Spices are functional foods which have played a vital role in the lifestyle of people from certain parts of the world. A spice can be a seed, root, leaf, stem, bark, fruit or any other part of plant. Apart from their role in making food taste good over the years, they are also used as colouring agents, flavouring agents, food preservatives and additives and as medicines for treating human ailments. Spices have infinite health benefits. The history of spices is the history of human civilization itself as many spices like pepper, coriander, fennel, cumin, garlic, etc. are known to be used for health problems since ancient Egypt, China, Greece, Arabia and many other countries. Spices were also used for trade in ancient times. Spices such as black pepper, cinnamon and cardamom have been in use by Indians for thousands of years, for both culinary and health purposes. India is blessed with a varied climate, therefore each of its state produces some spice or the other. In the present work medicinal values of some selected spices have been reviewed.

Keywords: Spices, functional foods, medicinal values, health benefits

ISBN: 978-93-5382-353-5


Page| 92

Physico-chemical Analysis of Honey Collected from Shirpur (Jain) Region

S N Tayade1, V N Lohiya1 and S B Gaikwad2

1Department of Zoology, 2Department of ChemistryLate Pundalikrao Gawali Arts and Science Mahavidyalaya, Shirpur (Jain), Washim

Abstract: This study was intended to estimate the physico-chemical parameters of honey samples collected from natural hives in Shirpur (Jain) region. A total of 20 natural hives were collected and extracted for honey. Samples each weighing 200 gm were taken for evaluation of physico-chemical parameters like, colour, electrical conductivity, moisture, hydroxymethyl furfural, free acidity, diastase, ash content, water insoluble solids. Colour parameter exhibited variations between honeys from different samples. The average values obtained for electrical conductivity (0.40 mS /cm) and pH (5.07) indicate that the analysed honeys came mainly from nectar. Moisture was low, with an average value of 12.71%. The HMF content was very low and ranged from 0.8 to 12.82 mg/kg. Moisture, HMF and free acidity values show good maturity and absence of undesirable fermentation in all the samples. Diastase activity had a mean value of 11.80 units on the Gothe scale. Results obtained in this study, indicate that honeys produced in this region is of excellent quality parameters according to international standards. Keywords: Natural honey hives, physico-chemical analysis, quality parameters, Shirpur (Jain)

Effect of Different Plant Hormones on Callus Induction in Catharanthus roseus (Apocynaceae)

Sachin R. Nirpane1 and Narayan B. Pandhure2

1Department of Botany, S.B.E.S. College of science, Aurangabad2Plant Tissue Culture Laboratory, Department of Botany, Dr. Babasaheb Ambedkar Marathwada

University, Aurangabad

Abstract: The use of Catharanthus roseus in cancer treatment is well known in India and the plant is valuable for its pharmaceutical properties. The present study describes callus induction of the plant Catharanthus roseus using internodal explants and the influence of different plant hormones like 2,4-D, kinetin, IAA, BAP on the growth of calli. Internodes were proved to be the best explant for culture, which were grown on MS basal medium (Murashige and Skoog, 1962) with different concentration of various growth regulators. The standard plant tissue culture protocol for callus culture was adopted. The highest efficiency of callus formation was observed in the medium containing different concentration of 2, 4-D and kinetin.Keywords: Catharanthus roseus, tissue culture, plant growth regulators, callus

ISBN: 978-93-5382-353-5


Page| 93

Rose Leaf Disease Detection using Deep Learning and Convolutional Neural Network

Varsha J. Sawarkar, Seema KawathekarDepartment of Computer Science & IT, Dr. Babasaheb Ambedkar Marathwada University

Rose plant is used to process for a research in this paper. Leaf disease detection is the input for to prevent the losses in the farming and also the product. Diseases decrease the efficiency of plant, which restricts the plant growth and also loss the quality and quantity. In this paper the approach is to the progress of rose leaf disease detection model that is based on basic image classification, by the use of deep CNN. For detection on rose leaves we used here the image processing and deep learning techniques. Deep learning is the exact and precise model for the plant disease detection. Infected leaves are collected and labeled as per the diseases finding on it. Processing of taken image is performed along with the pixel wise operation to get better the image information. Extracting the features and fit into the neural network. By the detection with CNN in image processing is the success for representing the possibility of this approach in the category leaf disease detection.Keywords: Convolutional neural networks, deep learning, Image processing, Plant disease, Rectified Linear Units

Fungal Pollution on Some Post Harvested Tropical FruitsR. B. Kakde

Department of Botany, Swami Vivekanand Senior College, Mantha

Abstract: In India, fruits have been found to be infected with several diseases in the field as well as very significantly in the transport and storage. Post-harvest losses in fruits might be due to perishable nature of fruits, improper physiological maturity, time and technique of harvesting during packaging and transporting. These are responsible for enormous qualitative and quantitative losses of fruits in the market. The fruit infection phase of the disease can result in serious economic loss. A post-harvest fruit and food loss constitutes a vast complex of physical and biological changes due to microorganisms like fungi and bacteria. An alarming percentage of fruits get spoiled due to parasitic and saprophytic fungi. Considering this fact a study was undertaken to understand responsible fungi to cause pollution to the tropical fruits like mango, papaya, grapes, banana and custard apple. In the present research work, numbers of fungi were isolated with respect to different varieties of fruits. These fungi are Alternariaalternata, Aspergillusflavus, Aspergillusfumigatus, Aspergillusniger, Botrytis cinera, Botryodiplodiatheobromae, Colletotrichumgloeosporioides, Colletotrichummusae, Curvularialunata, Fusariumequiseti, Fusariumoxysporum, Fusariummoniliforme, Fusariumsolani, Gloeosporium papaya, Gloeosporiumapocryptum, Macrophominaphaseolina, Penicilliumdigitatum, Phoma sp., Phomacaricae, Penicilliumchrysogenum, Phytophthoranicotianae, Rhizopusstolonifer and Verticilliumtheobromae.

Keywords: Storage fungi, post-harvest loss, fruit varieties

ISBN: 978-93-5382-353-5


Page| 94

Antibacterial Effect of Silver Nanoparticles on Multi Drug Resistant Microorganism

Lynn D’Lima, Manju Phadke and Vishal Dev Ashok

Abstarct: There is an undying need for inexpensive, energy efficient and eco-friendly protocols to produce biocompatible nanoparticles to be used in the fields of medicine. To address this issue, an exploratory study was performed to produce silver nanoparticles (AgNPs) using a novel strain of Kitasatospora albolonga isolated during the course of the study. The objective of this study was to identify the antimicrobial activity of AgNPs against Multi Drug Resistant (MDR) Pseudomonas aeruginosa.The presence of the synthesized AgNP was confirmed by UV-Visible spectroscopy, Dynamic Light Scattering (DLS), and Scanning Electron Microscopy (SEM). The UV-Visible spectroscopy performed on the synthesized nanoparticles, showed a localized surface plasmon resonance (LSPR) at approximately 420 nm. The DLS and SEM micrographs showed spherical particles where a majority ranged between 10 to 50 nm in size. The biosynthesized silver nanoparticles were tested for antimicrobial activity against MDR P.aeruginosa. The zone of inhibition of AgNP impregnated filter paper discs was compared with various commercially available antibiotic discs, alone and in combination with AgNP.Most of the commercially available antibiotic discs did not inhibit the growth of the MDR strain alone or in combination with AgNP.The study to check the synergistic action between the AgNPs andthe drugs was performed using the checkerboard assay. The synergistic action was found to be the best between the AgNPs and Carbenecillin drug against the MDR hospital isolate.The efficacy of this combination proved to be a lethal and viable option against the MDR P. aeruginosa.Exploring nano-biotechnology in therapy could improve patient care and reduce the copious amounts of potent antimicrobials administered there by reducing the incidence of drug resistance through multi directional antimicrobial action.Keywords: AgNPs, P.aeruginosa, synergism

Agro-pesticide Induced Anemia in Freshwater Fish Nemacheilus botiaJ. D. Vasait

Dept. of Zoology, M.G.V’s,M.S.G. Arts, Science and Commerce, College Malegaon camp, Nashik

Abstract: The effect of monocrotophos at respective sublethal concentrations in February, May and July months for 7 and 14 days was studied on some hematological abnormalities. Hb content, TEC count markedly reduced and TLC count increased.

Keywords: Monocrotophos, Haemoglobin, TEC, TLC, Nemacheilus botia

ISBN: 978-93-5382-353-5


Page| 95

Effect of Homoeopathic Medicine Mercuriuscorrosivus against the Disease Coccidiosis of Broiler Chicks and Examine the Histochemical

Changes in Intestinal TissueS. T. Naphade1, S. R. Naphade2 and C. J. Hiware3

1Dept. of Zoology, Yeshwantrao Chavan Arts, Commerce and Science College, Sillod, Aurangabad

2Dept. of Zoology, Dagadojirao Deshmukh College, Bajajnagar, Waluj, Aurangabad3Dept. of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Aurangabad

Abstract: The present communication deals with the study of effect of different potencies of homoeopathic medicine Mercuriuscorrosivus against experimental caecal coccidiosis of broiler chicks, (by induced the dose of 50,000 sporulated oocysts of Eimeriatenella). The treatment of different potencies of Mercuriuscorrosivus was administered to four infected group of broiler chicks. During the experimental period histochemical examination of intestinal tissue were undertaken in different group of broiler chicks (treated and untreated) and observed that the histochemical examination particularly lipid content of the intestinal tissue from all group of broiler chicks have the lipid content in their tissue. The concentration of lipid was variable from different region of the intestinal tissue and found different traces like low, moderate and high traces of lipid content in the tissue of different group of broiler chicks. From the above observation it is concluded that the intestinal tissue has the accumulation of lipid and is detected through the weak or strong reaction, i.e. stains black or brown. The amount of lipid is high in the healthy uninfected intestinal tissue whereas the amount is decrease is heavily infected and untreated groups. The lipid content in intestinal tissue of treated groups of broiler chicks has relatively high traces of lipid than untreated group. From the histochemical examination it is clear that parasitic stages could able to acquire the lipid from the host tissue whereas the treated groups has also shown the histochemical changes in groups treated with homoeopathic medicine Mercuriuscorrosivus and allopathic amprolium, other related aspects are also discussed in the text. Keywords: Homoeopathic medicine, Coccidiosis, Broiler chicks, Histochemicalchanges, Intestinal tissue

ISBN: 978-93-5382-353-5


Page| 96

Histopathological Changes in Rohu, (Labeo rohita) from Koradi LakeDeshpande A. S.

Department of Zoology, Chintamani College of Science, Pombhurna, Chandrapur

Abstract: The present study was conducted to examine contamination and the hazardous effect of heavy metal from the water body and tissues of the freshwater fish, Labeo rohita (Rohu). The heavy metals like As, Zn, Pb, Cd, Co, Ni, Mn, Fe, Cr, Al, and Cu were observed in water body adjacent to thermal power station in varying quantities that indicates the presence of heavy metal in water body. The histological changes in fish tissues were showed various structural changes, which point out towards deleterious effect of thermal power plant effluent on the freshwater fish L. rohita which might be due to the state of stress caused by exposure to metals.Keywords: Heavy metals, contamination, L. rohita

Evaluation of Ivy Gourd Genotypes in Maharashtra Region of IndiaV. S. Chatage

Department of Botany, Kai. Rasika Mahavidyalaya, Rasika Campus, Borol Road, Deoni, Latur

Abstract: Evaluation trial was conducted in ivy gourd genotypes collected from different parts of Maharashtra States (Aurangabad, Beed, Jalna, Pune, Nilanga, Udgir etc.) during the winter seasons of 2010 and 2013. The experiment was laid out in Randomized Block Design with three replications at the Experimental farmer’s fields, Department of Botany, Arts, Science and Commerce College Naldurg, Tq. Tuljapur. Dist. Osmanabad. Morphological chara cterization of 10 genotypes revealed significant variation among the genotypes. The mean performance of genotypes for quantitative traits revealed that the genotype IG-05 recorded maximum for fruit characters viz., fruit length (10.2 cm), fruit weight (12.00 g) and fruit yield per plant (14.07 kg). Whereas the genotype IG-09 exhibited the highest number of primary branches (7.16), fruit width (4.00 cm) and number of fruits per plant (400). Promising genotypes were IG-01, IG-07 and IG-04

Keywords: Ivy gourd, genotypes, high yielding, promising

ISBN: 978-93-5382-353-5


Page| 97

Screening of Endophytic Fungal Extract of Nerium Oleander L. for Insecticidal Activity against Callosobruchus Chinensis L. (Coleoptera:

Bruchidae)Jadhav P. N.1 and Pardeshi A. B.2

1Arts, Commerce and Science College, Nandgaon2P. G. Department of Zoology, Deogiri College, Aurangabad

Abstract: Endophytic fungi are microorganisms that live inside the plant without causing any overt negative effect on plant tissues; rather they protect the host plant from pests and diseases. The insecticidal activity of endophytic fungal extract of leaf of Nerium oleander against pulse beetle, Callosobruchus chinensis was studied. Endophytic fungi were isolated from the leaf of Nerium oleander and various concentrations of methanol and ethyl acetate crude endophytic fungal extracts were tested for their effect on oviposion, adult emergence from seeds and mortality of the store grain pest, Callosobruchus chinensis. Observations were made on the number of eggs laid on seeds treated with endophytic fungal extracts, adult emergence from seeds of Vigna unguiculata amd mortality of adults exposed to treated seeds. The number of eggs laid and the adults emerged from seeds treated with endophytic fungal extract were less than from untreated seeds.The mortality increases with increase in concentration of endophytic fungi. The methanol solvent extract showed more insecticidal property against C. chinensis due to the secondary metabolites of endophytic fungi. Statistical variance, 95% confidence limits and regression equations are presented.Keywords: Callosobruchus chinensis, endophytic fungi, mortality, Nerium oleander

Comparative Study of Cestode Parasites of Fresh Water Fishes in Dudhna Dam at Partur, Dist. Jalna, Maharashtra

Ravi Pradhan

Department of Zoology, Lalbahadur Shastri Sr. College, Partur, Jalna

Abstract: Present study during the year (Jun 2017 to May 2019) deals with fresh water fishes found in the Dudhana dam at Partur Dist. Jalna (Maharashtra), India. Author have collected 450 species of fresh water fishesi.e, Mastacembellus armatus, Puntata, Clarius batrachus, Labeo rohita, wallagoattu abundantly. Among them 150 fishes were found infected with the cestode parasitesViz., Lytocestus ,Senga,Silurotaenia, Circumonchobothrium and Gangesia species. The investigation shows the abundance of fresh water, Prevalence, Dominance of the species and mean Intensity. Keywords: Fresh waterfish, Circumonchobothrium, Gangesia, Silurotenia

ISBN: 978-93-5382-353-5


Page| 98

In Vitro Antioxidant and In Vivo Antiinflammatory Studies of Hydroalcoholic Leaf Extract of Sauropus androgynus (L.) Merr

Amitha Prasad, S. R. Suja and R. PrakashkumarEthnomedicine and Ethnopharmacology Division, Jawaharlal Nehru Tropical Botanic Garden

and Research Institute, Palode, Thiruvananthapuram

Abstract: Sauropus androgynus (L.) Merr (Euphorbiaceae) locally known as Veli Kheerai, is a popular leafy vegetable in South and Southeast Asia and is notable for its high yields and palatability. The dietary antioxidant micronutrients accumulated in fresh fruits and vegetables, particularly leafy vegetables, promote good health by assisting in preventing cancer and high blood pressure, stimulating the immune system, metabolism and tissue regeneration. Excessive amount of free radicals can lead to cell injury and death, which results in many diseases such as cancer, stroke diabetes, etc. Inhibition of free radical scavenging property serve as an important therapeutic consideration in development of anti-inflammatory agents. The aim of the present study is to evaluate the antioxidant activity and anti-inflammatory effect of hydroalcoholic leaf extracts of Sauropus androgynus.The plant extracts were prepared using three different solvent systems Ethanolic, Hydroethanolic and aqueous and dried extract were subjected to in vitro antioxidant activity .The antioxidant activity assays includes DPPH and reducing power, were carried out. The antioxidant assays revealed that hydroalcoholic showed significant presence of phytochemical constituents.Anti-inflammatory studies were conducted in Wistar Rats of weight (100-200g) and different dose of 50% hydroalcoholic extracts were administrated orally. The extract 150mg/kg produced a dose related inhibition of Carrageenan-induced paw oedema and Formalin induced paw oedema in rats. The 50% hydroalcoholic extract has shown significant inhibition of paw oedema at dose of 150mg/Kg with-respect to standard Indomethacin. Thus the results of the present study revealed the anti-inflammatory and anti-oxidant potential of Sauropus androgynus, a source of dietary anti-oxidants, which can be served as a anti-inflammatory agent. These activity may be due to presence of phytochemical constituents such as phenolics, flavonoids,etc. Further studies are needed to explore the anti-inflammatory models in different models and its mechanism of action.Keywords: Sauropus androgynus, DPPH- 2, 2-diphenyl-1-picryl-hydrazyl-hydrate

ISBN: 978-93-5382-353-5


Page| 99

Comparative Phytochemical Screening and Evaluation of Antioxidant Activity of Phyllocephalum rangacharii (Gamble) Narayana, an

Ethnomedicinal PlantAswathy V. Nair, S. R. Suja and R. Prakshkumar

Ethnomedinine and Ethnopharmacology Division, Jawaharlal Nehru Tropical Botanic Garden and Research Institute, Palode, Trivandrum

Abstract: Phyllocephalum rangacharii (Gamble) Narayana (Asteraceae) is an erect, slender herb which is endemic to southern Western Ghats, is used medicinally by Tribal communities of India. The objective of the study is to identify the various secondary metabolites responsible for the pharmacological activitiy and to evaluate the antioxidant potential of P. rangacharii. Therefore the present study was designed to screen the presence of different bioactive phytoconstituents in ethanolic, hydroethanolic and aqueous extract of P.rangacharri. The total phenol, total flavonoids, total tannin contents were determined using spectrophotometric method. The preliminary phytochemical analysis revealed the presence of alkaloids, flavonoids, tannins, phenols, saponins, terpenoids flavonoids and coumarins in the extracts. Hydroethanolic extract showed the presence of highest number of secondary metabolites and aqueous extract showed the least number. Hydroethanolic extract showed the highest total phenolic content and total tannin content when comparing to other two extracts. Total flavonoid content is comparable in ethanolic extract and hydroethanolic extract. Antioxidant activity of the three extracts were evaluated using different 2,2-diphenyl-1-picryl hydrazyl (DPPH) method. Hydroethanolic extract showed significant antioxidant activity than the other two extracts. The bioactivities of plant may be due to presence of various phytogenic chemical compounds and significant antioxidant potential. The qualitative and quantitative phytochemical analysis and antioxidant studies also substantiate the therapeutic use of P. rangacharii by the indigenous system of medicine.Keywords: Phyllocephalum rangacharii, free radical scavenging, ethnomedicinal value

ISBN: 978-93-5382-353-5


Page| 100

Detection of MDR Pseudomonas aeruginosa Isolates Obtained from Burn and Wound Infection in Western Yemen

Mahfouz Nasser1 and Arun S. Kharat2

1Dept. of Biotechnology, Dr. Babasaheb Ambedkar Marathwada University, Osmanabad2Internal Quality Assurance Cell (IQAC), Jawaharlal Nehru University, New Delhi

Abstract: P. aeruginosa represents one of the most common pathogens in burn wound infections. The antibiotic-resistant Pseudomonas aeruginosa affecting burn wound patient is a severe problem with regional and global implications, which requires antibiotic susceptibility testing regularly. The main aim of this study was to determine of multi-drug resistance Pseudomonas aeruginosa isolates obtained from burn and wound infection in Hodiedah city western Yemen. A study was undertaken with two hundred burns and wound swabs from patients those were hospitalized to burn and Wound wards at The General AL-Thawra Hospital, Hodiedah Western Yemen. The isolates were cultured and identified by standard microbiological techniques and with the use of Kirby- Bauer disc diffusion antibiotic susceptibility testing was done.In the current study during the period from 1/7/2018 to 31/12/2018, (98) ninety-eight (49 %) out of 200 samples which collected were P. aeruginosa. The highest rate of infection P. aeruginosa documented with wound patients (75.5%).The highest incidence of 40.9% was found in the age group 20- 39 years.Antibiotic susceptibility testing showed that the highest level of antibiotic resistance was 85.7% of isolates exhibited resistance to gentamycin (10 μg), then 74.5 % to Tobramycin (10 μg) and 83.7% to Amikacin (10 μg). while 77.5 to Ceftazidime (30μg), 72.4 to Cefepime (10μcg), and 26.5% were to Aztreonam (30 μg).54.1% were resistant to piperacillin-tazobactam (100/ 10 μg), while 66.3% were resistant to a fluoroquinolone antibiotic Ciprofloxacin (5 μg). Resistance to carbapenems was 21.4 and 20.4% respectively to Imipenem (10μg) and Meropenem (10μg).65 (66.3%) of the 98 P. aeruginosa isolates were multidrug resistance (MDR).This is the first report documenting enhanced MDR P. aeruginosa isolates obtained from burn and wound infection in western Yemen.The presence of MDR P. aeruginosa in burn and wound wards increases the risk of transmission to patients leading to nosocomial infections. The prevention of nosocomial infections needs quick detection and requires antibiotic susceptibility testing regularly.Keyword: Multiple drug resistance, p. aeruginosa, burn and wound, Hodiedah

ISBN: 978-93-5382-353-5


Page| 101

Cloning and Functional Analysis of DREB1 Transcription Factor Involved in Drought-Responsive Gene Expression in Triticum aestivum

S. V. Kale and B. T. PawarResearch Centre in Botany, Shri Muktanand College, Gangapur, Aurangabad

Abstract: The transcription factors DREB1s/CBFs specifically interact with the DRE/CRT cis-acting element and control the expression of many stress inducible genes in many monocots and dicots. We performed in silico analysis, including the identification of the different abiotic probesets and genes encoding proteins for abiotic stress. The databases were searched that contain the microarray data for the genes, which were analyzed for wheat in order to check their expression level under different growth conditions.We have carried out insilico analysis to identify highly upregulated drought tolerant genes or transcription factors. The primers for identified dreb1 gene sequences were designed and the landraces collected were screened for the presence of the particular proein coding sequence. In one of the landrace the sequence for DREB1 gene was amplified. The amplified fragment was isolated and cloned in suitable vector to get transformed into the susceptible wheat germplasm. The gene was transformed using the reported protocol in wheat and transgenic events carrying drought tolerant gene were generated. The further analysis of its efficacy is in progress. This research will help to develop draught resistant wheat varieties, which highly required in areas like Marathwada. Keywords: DREB1, Drought-Responsive Gene, Triticum aestivum

New Species of the Genus Davainea (Cestoda: Davaineidae) from Gallus domesticus

D. P. PatilDepartment of Zoology, Bhagwan Mahavidyalaya, Ashti

Abstract: The scolex of Davainea alandensis n. sp. is small, dome shaped nrrow at the anterior and posterior end, broad in the middle with four suckers, with armed rostellum, suckers medium, oval arranged in two pairs, rostellum armed with 86 hooks with two rows. Neck short, Mature segments medium, broader than long. Testes 22 in number arranged in two fields, 8 in aporal half, 14 in poral half, all situated posterior 1/3rd region of the segment, post ovarian. Cirrus pouch large, flask shaped. Cirrus thin; Ovary medium, dumb bell shaped, distinctly bilobed, obliquely placed, situated in the posterior half and in the poral half of the segment. Ovarian lobes unequal connected with each other by an isthmus; Vagina thin tube posterior to cirrus pouch ; Genital pores small, oval, marginal, unilateral, placed anterior half of the segment.Keywords: Davainea alandensis n.sp., Gallus domesticus

ISBN: 978-93-5382-353-5


Page| 102

Cylic Changes in Ovarian Maturation and Histological Observation in Indian Major Carp Catla catla [HAM]P. R. More1, R. Y. Bhandare2 and D. L. Sonawane3

1Kai. Rasika Mahavidyalaya, Deoni, Latur2MGV’S Arts Science and Commerce College Surgana, Nasik

3Department of Zoology Dr. Babasaheb Ambedkar Marathwada University Aurangabad

Abstract: Annual reproductive cycle of female Catla catla was investigated during the (January 2009 December 2009) through gross and histological studies. GSI (13.00±9.30 %) was observed in June. On the basis of gross and histological studies, seven ovarian stages namely, i) immature/resting ii) regenerating iii) developing iv) maturing v) mature/gravid vi) regressing and vii) regressed were distinguished. Based on GSI studies, spawning seem to for a period (late June-early September). Histological studies revealed six stages of oocyte development namely i) chromatin nucleolar ii) perinucleolar iii) cortical alveolar iv) early vitellogenic v) late vitellogenic and vi) early germinal vesicle movement. The present study describes the morphological and histological changes in ovaries of Catla catla. The annual breeding cycle of the fish has been divided in to four stages concerned with the following month of year i.e. i) the preparatory phase (January – march) ii) pre spawning phase (April – June ) iii) spawning phase (July – September ) iv) post spawning phase. Keywords: Ovaries, Cyclic changes and GSI

Evaluation of Ivy Gourd Genotypes in Maharashtra Region of IndiaV. S. Chatage

Department of Botany, Kai. Rasika Mahavidyalaya, Rasika Campus, Borol Road, Deoni, Latur

Abstract: Evaluation trial was conducted in ivy gourd genotypes collected from different parts of Maharashtra States (Aurangabad, Beed, Jalna, Pune, Nilanga, Udgir etc.) during the winter seasons of 2010 and 2011. The experiment was laid out in Randomized Block Design with three replications at the Experimental farmer’s fields, Department of Botany, Arts, Science and Commerce College Naldurg, Tq. Tuljapur. Dist. Osmanabad. Morphological chara cterization of 10 genotypes revealed significant variation among the genotypes. The mean performance of genotypes for quantitative traits revealed that the genotype IG-05 recorded maximum for fruit characters viz., fruit length (10.2 cm), fruit weight (12.00 g) and fruit yield per plant (14.07 kg). Whereas the genotype IG-09 exhibited the highest number of primary branches (7.16), fruit width (4.00 cm) and number of fruits per plant (400). Promising genotypes were IG-01, IG-07 and IG-04.

Keywords: Ivy gourd, genotypes, high yielding, promising

ISBN: 978-93-5382-353-5


Page| 103

Process optimization and Storage Stability of Value Added Chicken Meatballs Added with Whey Protein Concentrate and Skim milk

Powder as a BindersS. N. Rindhe, S. U. Suryawanshi and D. B. Kamble

Department of Livestock Products Technology, College of Veterinary and Animal Sciences, Parbhani

Abstract: The present study was aimed to develop an acceptable quality chicken meatball with incorporation of milk protein as binders. Minced meat from broiler, spent hen and its combination incorporated with spices, condiments and binders used for preparation of chicken meatballs. The protein binders were found useful for improving the sensory quality as well as physic-chemical characteristics of chicken meatballs. The scores for all sensory attributes viz., appearance, flavour, juiciness, texture and overall palatability decreased gradually with incorporation of whey protein concentrate and skim milk powder up to 5% level. Addition of higher level (7.5%) of WPC and SMP showed significant (P<0.05) decrease in all sensory attributes. The product made by incorporation of WPC and SMP showed superior physic-chemical characteristic than control. During refrigerated storage, the scores for all the sensory attributes declined with the progress of storage. Similarly, TBA number, tyrosine value and microbial counts increased considerably throughout the storage period of 20 days but were within the spoilage limit. It is concluded that chicken meatballs made by using combination of broiler and spent hen meat incorporated with 5% whey protein concentrate and 5% skim milk powder could be stored safely in HDPE pouch for 20 days under refrigerated storage.

ISBN: 978-93-5382-353-5


Page| 104

Study of Effect of Broad Spectrum Antibiotic, Tetracycline on the Digestive Enzyme Invertase of Freshwater Bivalve, Lamellidens

corrianus (LEA)H. P. Nandurkar

Department of Zoology, Sant Gadge Baba Amravati University, Amravati

Abstract: The change in any aquatic environment hamper the organisms living in it, the change occurs due to different pollutants or any chemical added in the ecosystem leads to alter the metabolic activities happen in the body of animals. Thus, the whole enzyme profile of the organism changed to cope up the stress caused by these factors.In the present paper, the stress caused by one of the broad spectrum antibiotics, tetracycline is studied as the antibiotic therapy is given to the freshwater bivalves in water in artificial pearl culture after a little surgery for implanting bead in the mantle ovary and other tissues.The acclimatized healthy bivalves, Lamellidens corrianus were exposed to acute treatment (LC50/2) 369.10PPM upto 96 hours and to chronic treatment (LC50/10) 73.82 PPM of tetracycline concentration up to 21 days. The estimation of invertase was carried out in mantle, gills and digestive glands of L. corrianus. Noelting and Bernfield’s, (1948) method was used for the estimation of invertase.The freshwater bivalves, L. corrianus showed the decrease in invertase enzyme, a main enzyme in the splitting of carbohydrates. Gills showed minimum invertase activity than other tissues studied. The invertase activity was reduced in all selected tissues of L. corrianus on acute and chronic exposures of tetracycline. The decrease in invertase activity progressed at the function of time. Maximum decrease seen in digestive gland was 34.34% after acute treatment and 27.55 after chronic treatment indicating the importance of digestive gland to study as a tool for assessing the stress level caused due to antibiotic stress.Keywords: Lamellidens corrianus, invertase activity, tetracycline, stress

ISBN: 978-93-5382-353-5


Page| 105

Fibrinolytic Enzyme from Pseudomonas rhizosphaerae BIORD2Asha S. Salunke 1,2 and Arun S. Kharat 1,3

1Department of Biotechnology, Dr. Babasaheb Ambedkar Marathwada University, Subcampus Osmanaba, Maharashtra

2BioEra Life Sciences Pvt. Ltd., Survey Number 125, Mumbai - Bangalore Highway, Tathawade, Pune, Maharashtra

3Laboratory of Applied Microbiology, School of Life Sciences, Jawaharlal Nehru University, New Delhi

Abstract: Despite all the advancement in the research area, cardiovascular diseases (CVDs) remain major concern globally. With increase in the life style changes and exposure to add-on risks the quantum of CVDs has increased in past few decades and thus there is a vital need for a better drug to combat the CVDs. Fibrinolytic enzymes are looked upon as a significant therapeutic agent for CVDs in the current scenario. Many of the fibrinolytic enzymes are identified from the microbial sources considering the possibility of recombination to yield selective enzyme protein. Keeping the quest for identification of effective fibrinolytic enzyme from microbial source, screening of microbial cultures with potential fibrinolytic activity was done from environment samples. Pseudomonas rhizosphaerae BIORD2 was isolated and identified by 16s rRNA sequencing (GenBank accession number MK183748). Cultural conditions were optimized to yield higher fibrinolytic activity by one-variable-at-a-time approach. An extracellular enzyme with molecular weight 37 kDa was purified by acetone precipitation, anion exchange chromatography and gel filtration chromatography. The purified enzyme yield was 10.82% with specific activity 22.02 U/mg and purification fold of 4.8%. Purified fibrinolytic enzyme In-vitro showed complete dissolution of blood clot within 1 hour of incubation. Pseudomonas rhizosphaerae BIORD2 produces an extracellular fibrinolytic enzyme offering use of simpler extraction and purification methods with retaining higher specific enzyme activity. A further study on characterization of enzyme and testing efficacy of enzyme by other available In-vitro methods is in process.Keyword: Enzyme, cardiovascular diseases, blood clot, therapeutic agent

ISBN: 978-93-5382-353-5


Page| 106

Studies on Development and Storage Stability of Gizzard Pickle- An Innovative Meat Products Added with Various Levels of Acidulants

S. N. Rindhe and S. U. SuryawanshiDepartment of Livestock Products Technology, College of Veterinary and Animal Sciences, Parbhani

Abstract: The demand for traditional meat products is ever increasing with growing consumer’s response and awareness about the nutrition and quality. Chicken meat is comparatively more acceptable than other meat because of its flavour, ease of digestion, low fat content, high ratio of unsaturated fatty acids and also excellent source of protein and its contain omega 3-fatty acid. The present study was undertaken with a view to develop acceptable quality for gizzard pickle prepared from by-product gizzard of broiler meat soaked with different acidulants. Row gizzard were soaked in acidulants and allowed to refrigeration temperature for 24 hrs and then boiled, fried, mixed with spices, condiments and used for preparation of gizzard pickle. The acidulants were found useful for improving physico-chemical characteristics of gizzard pickle. The scores for all sensory attributes viz. appearance, flavour, juiciness, texture and overall palatability decreased gradually with soaking of citric acid and lactic acid. Soaking of gizzard in higher level of acidulants showed significant (P<0.05) decrease in all sensory attributes. Among the treatment the product prepared from acetic acid soaked with 1 percent level exhibited significantly higher score for all sensory attributes and physico-chemical characteristic. Gizzard pickle prepared by soaking of citric acid 1 percent level and lactic acid 2 percent showed gradual decrease in sensory attributes and physico-chemical characteristic. At ambient temperature, the scores for all the sensory attributes declined with the progress of storage. Similarly, pH, TBA number and microbial counts increased considerably throughout the storage period but were within the spoilage limit up to 40 days.It is concluded that gizzard pickle soaked with 1 percent acetic acid soaked could be stored safely in PET bottles for 40 days under ambient temperature storage (37 ± 10 C).

ISBN: 978-93-5382-353-5


Page| 107

Efficacy of Thiophanate Methyl on Pathogenic FungiSatpute S. B. 1, Vaidya S. R.2 and Vanmare D. J.3

1Dept. of Biology, Shiv Chhatrapati College, N- 3, Cidco, Aurangabad, Maharashtra2Department of Chemistry, 3Department of Botany, Vivekanand Arts, Sardar Dalipsingh

Commerce and Science College, Samarthnagar, Aurangabad

Abstract: The Percent control efficacy of Thiophanate methyl at various concentrations i.e 100-1000 ppm, on five pathogenic fungi viz. Fusarium incarnatum, Alternaria citri, Colletotrichum musae, Colletotrichum sp. and Gibberella avenaceum was studied. The percent inhibition of fungi significantly differed (0.01) among themselves, while it was non-significant at various concentrations of fungicide. On an average, there was 91.67 % inhibition of mycelial growth due to the treatment with 100 ppm fungicide. It significantly increased up to 95.91 % at 300 ppm concentration and then gradually up to 98.27 % at 1000 ppm concentration. Among the fungi 100% growth inhibition was observed in case of Alternaria citri, Colletotrichum sp. and Gibberella avenaceum at 100 ppm. In case of Colletotrichum musae 100 % inhibition was observed at 500 ppm. concentration of the Thiophanate methyl. Fusarium incarnatum was found to be relatively resistant to the fungicide which shows 91.36 % inhibition at 1000 ppm concentration of Thiophanate methyl. The differences in percent inhibition among the fungi were statistically significant. Keywords: Fusarium incarnatum, Alternaria citri, Colletotrichum musae and fungicide

Enzyme-laden Biowaste of an Industrial Microorganism for theSynthesis of Gold Nanoparticles and Biochemical Method for the

Synthesis of Silver NanoparticlesMehavish Fatima and Aniroodha V. Pethkar

Govt. Institute of Science, Nipatniranjan nagar, Caves Road, Aurangabad

Abstract: Biological agents such as plants and plant products as well as microorganisms are valuable tools for the reduction of metal ions to yield nanoparticles. These approaches are eco-friendly and economical, especially if they produce stable and monodisperse nanoparticles. A number of microbial enzymes such as amylases, proteases and lipases are commercially used in food, detergent and process industries. In the present studies, a soil isolate, identified as Bacillus subtilis, was found capable of producing the intracellular enzyme amylase with the potential for industrial use. Culture supernatants of the isolate grown in nutrient broth were found to synthesize gold nanoparticles by reduction of the metal ions. This was evident from the characteristic Surface Plasmon Resonance of the preparation at 520 nm. Studies were carried out to investigate the mechanism of reduction of the metal ions by the isolate. The ability of culture supernatants to carry out nitrate reduction pointed

ISBN: 978-93-5382-353-5


Page| 108

to the secretion of nitrate reductase enzyme by the isolate. Optimization of enzyme production was carried out by controlling the environmental and growth conditions. Since, enzyme-based processes are more controllable and predictable as compared to whole cells the enzyme was purified by salt fractionation followed by ion-exchange chromatography and then used for the enzymatic synthesis of gold nanoparticles. The enzyme was purified 7.066 fold with specific activity of 2.643 u/mg and yield of 35.30 % and showed a high efficiency of gold nanoparticles synthesis compared to culture supernatants and crude enzyme preparations. Further studies will be carried out on the use of the enzymatically synthesized gold nanoparticles for bio-medical applications. There is an increasing industrial demand for the development of eco-friendly methods for the synthesis of metal nanoparticles due to their potential applications in the biomedical and pharmaceutical fields. Traditionally, nanoparticles are being synthesized by chemical and physical methods with controlled sizes and required chemical purity. However, physical methods consumes high level of energy during the synthesis process and chemical methods use of plenty of toxic chemicals as well as production of hazardous wastes during the synthesis process limits their applications in medical fields. Hence, the development of eco-friendly and biocompatible process for AuNPs synthesis is requiredUsing bioinformatics tools such as molecular docking studies, the possibility of binding of the gold ions to the active site of nitrate reductase and conversion to gold nanoparticles was investigated. The binding possibilities and stability of query sequence (active site residues) and target (gold ions/nanoparticles) clearly pointed to the interaction of gold ions with the enzyme that culminated in the synthesis of nanoparticles. Actinomycetes have long been used for the production of important antimicrobial agents. However, these organisms have not been investigated for the ability to synthesize nanoparticles. As Emerging infectious diseases and the increase in incidence of drug resistance among pathogenic bacteria have made the search for new Antimicrobials inevitable. In the current situation, one of the most promising and novel therapeutic agents are the Nanoparticles.The unique physiochemical properties of the Nanoparticles combined with the growth inhibitory capacity against microbes has led to the upsurge in the research on nanoparticles and their potential application as Antimicrobials .The biosynthesis of silver nanoparticles (AgNPs) was carried out using actinobacteria isolated from IHM Rauzabagh, Dr Rafiq Zakaria campus, Aurangabad. The culture supernatant of selected strain IHM-11, was admixed with AgNO3 (1mM) and incubated at room temperature under dark conditions. Biosynthesized AgNPs were confirmed and characterized by using UV-visible spectroscopy and SEM-EDS analyses. The shape of synthesized AgNPs was characterized using SEM and confirmed as small spherical in nature. The biogenic AgNPs were found to enhance the inhibitory activity of commercial antibiotics such as ampicillin and carbenicillin against MDR bacteria, viz. S. aureus, Pseudomonas, B. cereus, and E. coli. Synergistic antibacterial effects were observed when the nanoparticles were tested in combination with commercially available antibiotics pointing to their potential use as antimicrobial agents.This method gives us the idea to investigate the mechanisms involved in nanoparticles synthesis, i.e. by enzymatic process and biochemical (metabolite-based). Further characterization of synthesised nanoparticles and use this nanoparticle for biomedical and environmental applications in progress.

ISBN: 978-93-5382-353-5


Page| 109

Interactive effects of Lead Acetate on Oxygen Consumption of Fresh Water Fish: Rasbora danicanius

J. V. Jagatkar and A. B. KasbeP.G. Dept. of Zoology, Vaidhynath Collage Parli-Vaijnath

Water is one of the most important requirements of all living beings for performing essential life function. With the rapid growth of industries in the century, pollution in natural wastes by industrial wastes has increased tremendously. Due to lack of treatment and improper mode of disposals, the water bodies are polluted and they carry deadly substances. The treatment of wastes water developed in advanced countries is highly mechanized and energy consuming and is neither appropriate nor financially justifiable for developing countries. Many chemical compounds and other potential pollutants produce adverse biological reactions at undetectable concentrations.The rate of oxygen consumption is indicative of stress physiology of the exposed fishes and thus can be used as a biochemical marker parameter of heavy metal pollution. Whereas oxygen extraction efficiency decreased over 24 hrs. of lead exposure. Extensive accumulation of lead in gills resulted in marked ultra-structural damages to the respiratory epithelium of the gill including swelling of secondary lamellae evidenced by changes to both lamellar region. The result indicates that the alteration in respiratory metabolism of exposed fishes is due to damages to respiratory organ and also to mitigate the stress caused by heavy metal pollutants.The fresh water fish Rasbora daniconius showed decrease in oxygen consumption when exposed to acute treatment to lethal concentration of lead acetate (LC50values of 96 hrs.) the concentration of lead acetate, The rate of oxygen consumption was at 24, 48,72, and 96 hrs of exposure to lead acetate were 0.1752, 0.1280, 0.1205 and 0.0875 respectively.It was found that oxygen consumption decreased after acute treatment as compare to that of control fishes. The rate of oxygen consumption ranged from the oxygen consumed by fish was measured at an interval of 24, 48, 72 and 96 hrs. of interval was found that oxygen uptake significantly decreased. In lead acetate treated animals at the end of 24, 48, 72 and 96 hrs. were (28.64% P<0.05), (43.12%P<0.01), (43.96%P<0.05), (56.47%P<0.001) ml/gm/hrs/lit respectively.In conclusion rate of oxygen consumption can be used as a biochemical parameters to assess the impact of heavy metal pollutant on the freshwater fishes. In the present investigation therefore impact of lead acetate exposure on oxygen consumption of R. daniconius has been documented.Keywords: Lead acetate, oxygen consumption, Rasbora danicanius

ISBN: 978-93-5382-353-5


Page| 110

Secondary Metabolites, Ros and Antioxidants Act as Growth Regulator and Chemical Defence against Pathogens in Plant Foliar Galling System

Sampurna Roy and Amlan DasDepartment of Zoology, University of Calcutta, Kolkata

Abstract: This paper reports the contents of the foliar gall metabolites of Alstonia scholaris, Mangifera indica and Ficus carica infestated by tiny dipteran fly. For the present study we have taken non-gall (NG) as control, immature gall and gall part of the plant species. The assayed parameters were total phenol, tannin, lignin, ROS and antioxidants. Differences in the assayed parameters were evaluated by One-way ANOVA followed by multiple tests of Tukey and differences were considered significant at p<0.05. High phenol, tannin and lignin contents in gall leaves suggested many kinds of stress response has been related to the defense mechanism which thereby inhibits the oviposion of insects and also prevent against the natural enemies. The higher amount of ROS level at the pathogen attack site of the gall tissue cause host cell death which prevents further spread of invasive pathogens. The antioxidant activities were examined by nitric oxide scavenging, hydroxyl scavenging, superoxide dismutase scavenging and so on. High amount of antioxidants in gall tissue provide first line of defense against over accumulation of ROS. The results of the present investigation establish the co-occurring role of phenol, ROS and antioxidants which enact in regulatory mechanism for tissue proliferation thereafter neoplastic growth of plant tissues against enemies. The observed biochemical changes make sure the hypothesis that galls act like metabolic machinery.Keywords: Gall, Phenols, lignin, tannin, ROS, Oxidative burst, Antioxidant.

“Bio”-interface in the Synthesis and Applications of NanoparticlesAniroodha V. Pethkar

Government Institute of Science, Nipatniranjan Nagar, Caves Road, Aurangabad

Abstract: Nanoparticles, the building blocks of materials and devices based on nanotechnology, are distinct on account of the size dependent alteration in their properties. The interest in nanotechnology is due to the possibility of newer and varied applications of such materials. Two approaches are used for the sythesis of nanoparticles. The top-down approach usually employes physical forces to break down bulk material yielding nanoparticles. Bottom-up approach attempts to build nanoparticles by assembling atoms/ions/molecules using chemical reactions. Physical methods are energy and inventory intensive while chemical methods generate toxic byproducts and residues. The synthesized nanoparticles need to be stabilized in order to prevent aggregation and conversion back into bulk material. This presentation focuses on our attempts to synthesize metal nanoparticles using green approaches. Iron oxide nanoparticles (10-30nm) were synthesized using Bacillus pumilus isolated from

ISBN: 978-93-5382-353-5


Page| 111

agricultural soil. The ability of this urealytic bacterium to tolerate and degrade high levels of urea (4% w/v) was used to generate alkalinity (pH >9). Reaction of ferrous and ferric iron in alkaline conditions yielded superparamagnetic nanoparticles that were responsive to magnetic field. Natural protein capping provided stability to the biogenic nanoparticles. The nanoparticles were functionalized and used in bioseparation experiments; wherein, conjugated enzyme (pectinase) was separated in magnetic field post reaction and reused multiple times. Pectinase activity of the conjugated enzyme was found to be 0.386 U, which was higher than that of the unconjugated enzyme (0.33 U). Bacterial genomic DNA was also isolated in pure from E. coli DH5α in a single step and the magnetic nanoparticles could be reused. In another example, extracellular nitrate reductase enzyme from Bacillus spp. was purified and used for the synthesis of silver and gold nanoparticles. The antimicrobial property of enzymically produced silver nanoparticles was studied. Molecular docking studies revealed the interaction of gold ions at the active site of the enzyme. Silver nanoparticles were also synthesized and stabilized using an algal biopolymer pointing to the potential of algae in nanotechnology. Copper nanoparticles synthesized using plant extracts were found to possess antimicrobial properties. Attempts are being undertaken in order to test the copper nanoparticles for therapeutic applications. Biopolymers obtained from plant material such as Aloe vera leaves and Hibiscus flowers were found to stabilize chemically synthesized iron sulfide nanoparticles. The nanoparticles were used for reductive degradation of various toxic dyes including methyl orange, acid black, methylene blue, etc. In an upflow continuous packed bed column nanoreactor, about 15 bed volumes of methyl orange solution (20mg/L) were treated with efficiency exceeding 90% and specific dye removal capacity of 13.70 mg/g nanoparticle. The calculated mass transfer coefficient was found to be 15 x 10-5L/mg/min. A fungal biopolymer obtained from Itajahiya spp. was also used to stabilize iron sulfide nanoparticles that could efficiently degrade lindane, a toxic pesticide. The mechanism of dye removal and degradation was investigated using chemical analyses and kinetic studies. The results demonstrated the applicability of the system for treatment of large volumes of the wastewater. The laboratory synthesized nanoparticles were characterized using scanning electron microscopy, infrared spectroscopy, particle size analyzer, vibrating sample magnetometer, x-ray diffraction, etc. In carefully designed experiments, it was demonstrated that the nanoparticles synthesized in the laboratory were non-toxic. Moreover, the treated dye samples also had remarkedly reduced toxicity. Keywords: nanoparticles, biopolymers, degradation, antimicrobial, dye removal, wastewater

ISBN: 978-93-5382-353-5


Page| 112

Protein Content in Gills, when Subjected to Cerebralectomy in BivalveSangeeta B Dongre

Dept. of Zoology, Government Vidarbha Institute of Science and Humanities Amravati, Maharashtra

Abstract: Bivalves amongst the aquatic organisms of the commercial important mussel constituents a remarkable component in the littoral ecosystem and generate considerable research interest by virtue of their wide spread distribution and specific ecological adaptation and edible value. In bivalves the endogenous factors like the endocrine gland secretions and the exogenous factors like season, temperature, salinity, food availability etc, plays vital role in regulating the growth and metabolism. Proteins are most abundant intracellular macro-molecules and constitute over half the dry weight of most organisms. They occupy a central position in the architecture and functioning of living matter. Adult bivalves in 95-110 mm shell length were selected for laboratory experiments. The animals were divided into 3 groups, the non- operated served as control and the two were experimental. The results obtained were significantly high 0.57± 0.35 (36.45%) 2.65± 0.11 (64.87%) 6.72± 0.11 (31.05)10.70± 0.01 (15.15%) in control as compare to experimental . 0.46± 0.02 (51.36) in experimental 3.43± 0.32 (77.02%) ,4.23±0.19 (64.03%) 11.76± 1.008 (93.96%) . Thus, it can be concluded that cerebral ganglia may plays an important role mostly inhibitory one, in regulation of metabolic rate and organic reserve from different body component, On general experiment showed that the effect was pronounced in unilaterally cerebralectomized group animals, these biochemical content can be utilized for freshwater aquaculture to develop at commercial purpose in provision of adequate nutrition for growing population.

Study of Ethanolic Extracts of Carica Papaya to Develop Potent Biological Agent against Fusarium Wilt of Lentil

Pawan L. Deshmukh and U. P. MogleDepartment of Botany, JES College, Jalna

Abstract: Lentil is one of the most important crop produced in India, and is one of the leading country to produce it in large amount after Canada. The crop of lentil is suffers from certain fungal infections one of such is fusarium wilt of lentil which reduces the overall yield of the crop every year. Ethanolic extracts of Carica papayaleaves were tested against Fusarium oxysporum, causing Fusarium wilt of Lentil. The leaves were found to have antifungal activity. During the study alcoholic extracts of Carica papayawas characterized with the help of Gas chromatography and High resolution Mass Spectroscopy (GC-HRMS). It was found that two compounds were present in the leaf extract of Carica papaya.Keywords: Ethanolic extract , Papaya, Fusarium

ISBN: 978-93-5382-353-5


Page| 113

Control of Seed-borne Alternaria Species Using Extraction of Medicinal Plant

Sulochana Ramsing Rathod1 and Surekha Anil Saraf2

1Dept.of Botany, 2Dept.of Zoology, Govt.College of Arts and Science, Aurangabad

Abstract: Seed-borne Alternariaspecies are isolated from different variety of cereals and pulses seeds. Five different variety of cereals and pulses seeds are used. Alternariaalternatais most frequently isolated species on all five type of cereals and pulses followed by A. tenuissima, A. crassa. A. triticum etc. determined by plating the seed on both agar plate method (APM) and Standard blotter paper method (BPM) and medicinal plants leaf extracts. Neem ,Tulsi, Hibiscus, in different concentration of leaf extract were used from 10% to 50% concentration were evaluated against two common species of Alternaria isolated from cereals and pulses. The result revealed that all the plant extract inhibited the growth of mycelium. Effect of all type of plants were varied in various concentration of leaf extract. Whereas lowest growth inhibition record in 10% leaf extact concentration. Seed treatment at 30% leaf extract concentration in all the species are found to be effective in reducing the relative of seed-borne Alternaria species occurring on the seeds and also increase percentage of seed germination. Leaf extract of medicinal plant inhibited growth of Alternariaspecies on seed and useful for seed germination.Keywords: Seed-borne Alternariaspecies, leaf extract, seed treatment

Synthesis, Characterization and Biological Activities of Some Novel Schiff Bases Derived from 3-Acetyl-4-Hydroxy-2H-Chromen-2-One and

5-(4-Aryl)-1,3,4-Thiadiazol-2-AmineJadhav R. L.1, Joshi H. U.1 and S. B.Ubale2

1Department of Chemistry, Swa. Sawarkar College, Beed2Department of Chemistry, R. B. Attal College Gevrai

Abstract: Chromen-2-onederivatives are one of the most significant families of natural productsand in synthetic organic chemistry. They have been widely used as starting materials or intermediates in the pharmaceutical, perfumery and agrochemical industries. Thaidiazoles are the important class of heterocyclic compounds with significant biological applications. In view of the high degree of bioactivity shown by chromen-2-oneand thiadiazole, the present study is focused on the synthesis of some novel Schiff bases containing chromen-2-one and amino thaidiazole rings. In vitro biological screening effects of the investigated compounds were tested against bacterial species Staphylococcus aurous, Escherichia coli, Salmonella typhi, Bacillussubstilis by Agar cup method and fungal species Aspergillusniger, Penicilliumchrysogenum, Fusoriummoneliforme and Aspergillusflavus by the poison plate method.Keywords: Chromen-2-one, aminothiadiazole, Shiffs base and antimicrobioal activity

ISBN: 978-93-5382-353-5


Page| 114

Diversity of Bird’s in “Gautala Autramghat Reserve Forest” at Aurangabad, Maharashtra

Amrin N. Mirza1 and Satish. S. Patil2Dr. Babasaheb Ambedkar Marathwada University, Department of Environmental Science

Abstract: Birds are the most widespread, diverse, and mobile living organisms on the earth. Birds are among the greatest monitors of environmental variations. Birds have played a unique role in the protection, growth, and restoration of natural environs and thus their significance and importance in the preservation of clean and healthy surroundings are of a high order. Birds are part of the natural habitation of the Indian Subcontinent, an area teeming with winged residents. The food of the birds varies and is different not only in respect of the different birds but also in respect of different seasons. Among the birds recorded in the study area, some of the insectivores and other dominating types included mixed diet birds, omnivores, carnivores, granivores, and frugivores, respectively. Species richness was greatest in the early winter and generally less in different seasons. The study is carried out to obtain some preliminary checklist of the bird community in Gautala Autramghat reserve forest. Survey of birds conducted and checklist has been prepared (2017-2018) to find out the status of birds populace in a region. The Gautala Autramghat reserve forest is a dry deciduous forest, rich in diversity of birds and consists of the high diversity of flora and mountain region, rocky areas and small lakes, grassland and plain areas and Overall 71 bird species belonging to 38 families were recorded. There were 62 residents, 7 winters migratory, one breeding migratory birds, and one Non-resident bird, respectively. The majority of the bird species were seen in winter because of more nourishment accessibility and good climatic conditions for nesting and roosting. In the present study at Gautala Autramghat reserve forest, identification was done with respect to various ecological aspects of avian diversityKeywords: Birds diversity, Gautala Autramghat reserve forest, Resident Birds, Environment

international multidisciplinary research conference on

Documents