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Drug resistance - related mutations in multidrug - resistant Mycobacterium
tuberculosis isolates from diverse geographical regions
Senia Rosales-Klintz, Pontus Jureen, Aksana Zalutskayae
Journal Reading Diska Astarini (I11109083)
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Introduction
• Globally, it is estimated that multidrug-
resistant tuberculosis (MDR-TB) affects
around 650,000 patients .
• Mismanagement of MDR-TB treatment
→ extensively drug-resistant TB (XDR-
TB)
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Introduction
•Rapid and accurate detection of
MDR/XDR-TB is a priority.
•Several genetic biomarkers have
been identified and shown to be
related to phenotypic drug
resistance.
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Materials and methods
Sample of 120 clinical MDR M.tuberculosis samples collected from:
•Belarus -China•Honduras -Iraq•Iran -Romania•Uganda
•Both the invitro drug susceptibility testing(DST) and the DNA extraction were performed
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PCR amplification and sequencing
•The DNA extracts were amplified and
sequenced to identify mutations in :
• the rifampicin resistance-determining region
(RRDR) of the rpoB gene
• the katG gene ,the mabA-inhA promoter
region
•and the quinolone resistance-determining
region (QRDR) in the gyrA gene
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Conclusion
•Knowledge of the prevalence of
geographic specific mutations can
allow the development of in-
house, PCR-based methods for
targeting mutations relevant in a
specific setting.
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Conclusion
• Since there are clear geographical
differences in the presence and proportion of
resistance-related mutations, it is crucial to
include enough drug-resistant clinical
isolates from various parts of the world when
new diagnostic tools aimed at global
implementation are developed and evaluated.
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