labspec© software

LabSpec© SoftwareUSER GUIDE

Part Number: 31 087 064

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COPYRIGHT

Copyright © 1999 Dilor-Jobin Yvon-Spex, HORIBA group division. All rights reserved. No parts of this publication maybe reproduced, transmitted, transcribed, stored in a retrieval system, or translated into any language or computer language,in any form or by any means, electronic, mechanical, magnetic, optical, chemical, manual or otherwise, without prior writ-ten permission of Dilor-Jobin-Yvon - Spex, HORIBA group division (FRANCE).

NOTICE TO THE USER

This manual should not be construed as any representation or warranty with respect to the unit named herein. Occasionally,changes or variations exist in the unit that are not reflected in the manual. Generally, should such changes or variations existand affect the product significantly, a release note would accompany the manual. In such a case, be sure to read the releasenote before using the product.Trademarks: LabSpec is a registered trademark of Instruments S.A.; IBM, PC-XT, PC-AT, Windows 95 are registered trade-marks of International Business Machines Corporation; MS-DOS is a registered trademark of Microsoft Corporation.

Version 1.1Dilor-Jobin Yvon-Spex

All Rights ReservedPrinted in France

MM-NP/Labdoc.FM/0399 August 1999

Jobin Yvon-SpexHORIBA Group

Dilor S.A.

Dilor GmbH

ISA Inc.

ISA UK Ltd

ISA ITALIA Srl

ISA Nederland

16-18, rue du Canal

91165 LONGJUMEAU CEDEX (France)

Tel: (33) 01 64 54 13 00 - Telex: JOBYVON 602882 F

Fax: (33) 01 69 09 93 19 - (33) 01 69 09 07 21

Internet site: http://www.JobinYvon.com , http:// www.Isainc.com

244 ter, rue des Bois Blancs

59000 LILLE (France)

Tel: (33) 03 20 08 12 20 - Fax: (33) 03 20 08 12 28/29

Email: [email protected]

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Tel: (49) 062 51 40 42 - Fax: (49) 062 51 648 71

Email: [email protected]

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Tel: (1) 908 549 71 44 - Fax: (1) 908 549 51 25

2-4 Wigton Gardens - STANMORE

Middlesex HA7 1BG (Great Britain)

Tel: (44) 181 204 81 42 - Fax: (44) 181 204 61 42

Via Cesare Pavese 35/AB - 20090 OPERA (Milano) (Italy)

Tel: (39) 2 57 60 30 50 - Fax (39) 2 57 60 08 76

P.O. Box 56 -2400 AB ALPHEN a/d Rijn (Netherlands)

Tel: (31) 0/1720 33323 - Fax: (31) 0/1720 39532

LabSpec Software i - 2

http://www.JobinYvon.com

http://www.Isainc.com

Dilor-Jobin Yvon-Spex, Horiba group division

With its long experience in research and manufacture of scientific instruments (since 1819), the Dilor-Jobin Yvon-Spex equipment, division of HORIBA Group, uses state of art technology for optical,mechanical, electronic and computer management.

The result is unequaled comfort and flexibility of use, with less human error, for the kind of precisionand reproducibility of measurements that are required in laboratory work today.

Last but not least, Dilor-Jobin Yvon-Spex instruments are designed and manufactured with total usersafety in mind.

Our Internet Site: http://www.JobinYvon.comhttp://www.Isainc.com


http://www.JobinYvon.com

http://www.Isainc.com

Warranty

Dilor-Jobin Yvon-Spex, HORIBA Group division, guarantees each instrument it manufactures for aperiod of one (1) year, including parts and labor. Our obligations during this period will be limited tothe repair or replacement -at our discretion- of every instrument returned to the factory, shipment pre-paid, during a period of one (1) year from the date of delivery to the original purchaser, providing thatDilor-Jobin Yvon-Spex or its representative gives prior approval.


About the LabSpec User Guide

Your approach to the LabSpec User Guide depends on what you want to do and how much you alreadyknow.

The following list gives a brief description of each section of this manual.

1. Introduction2. Reference section4. «Tips» section5. Index


Contents

Icons list . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9

Menu Bar Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47


1.1 Icons list

Icon 1 : Standard cursor of work - - - - - - - - - - - - - - - - - - - - - - - - - page 13

Icon 2 : Peak elimination - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 13

Icon 3 : Shape correction - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 13

Icon 4 : Zoom - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 13

Icon 5 : Horizontal shift - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 13

Icon 6 : Vertical shift - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 13

Icon 7 : Intensity adjustment - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 13

Icon 8 : Shift - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 13

Icon 9 : Manual addition - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 13

Icon 10 : Manual multiplication - - - - - - - - - - - - - - - - - - - - - - - - - - - page 14

Icon 11 : Manual labeling of peaks- - - - - - - - - - - - - - - - - - - - - - - - - page 14

Icon 12 : Move peak maxima - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 14

Icon 13 : Fit peak width - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 14

Icon 14 : Delete active object - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 14

Icon 15 : Load a file- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 14

Icon 16 : Save the active object - - - - - - - - - - - - - - - - - - - - - - - - - - - page 14

Icon 17 : Cursor normalization - - - - - - - - - - - - - - - - - - - - - - - - - - - page 16

Icon 18 : Normalization - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 16

Icon 19 : Print page - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 16

Icon 20 : Information about object - - - - - - - - - - - - - - - - - - - - - - - - - page 16

Icon 21 : Multiwindow automatic spectra recording - - - - - - - - - - - - - page 17

Icon 22 : Mathematical treatments - - - - - - - - - - - - - - - - - - - - - - - - - page 17

Icon 23 : Baseline correction - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 19

Icon 24 : Correction- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 21

Icon 25 : Profile - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 23

Icon 26 : Filtering - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 26

Icon 27 : Fourier filtering - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 29

Icon 28 : Peak fitting - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 30

Icon 29 : Integral calculation - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 33

Icon 30 : Palette window - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 33

Icon 31 : Zoom - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 34

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Icon 32 : Spectral mapping procedure- - - - - - - - - - - - - - - - - - - - - - - page 35

Icons list

Icon 33 : Model procedure - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 36

Icon 34 : Video image - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 36

Icon 35 : Spectrum adjustment - - - - - - - - - - - - - - - - - - - - - - - - - - - page 36

Icon 36 : Spectrum recording - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 37

Icon 37 : CCD image adjustment - - - - - - - - - - - - - - - - - - - - - - - - - - page 37

Icon 38 : Spectral images recording - - - - - - - - - - - - - - - - - - - - - - - - page 37

Icon 39 : Data size- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 38

Icon 40 : Acquisition parameters - - - - - - - - - - - - - - - - - - - - - - - - - - page 41

Icon 41 : Detector parameters - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 42

Icon 42 : Morphological imaging - - - - - - - - - - - - - - - - - - - - - - - - - - page 43

Icon 43 : Calibration of the XY table - - - - - - - - - - - - - - - - - - - - - - - page 43

Icon 44 : Configuration - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 45

Icon 45 : Scheme- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - page 46

Icon 46 : Stop of spectra recording- - - - - - - - - - - - - - - - - - - - - - - - - page 46

Icon 47 : Different types of cursors - - - - - - - - - - - - - - - - - - - - - - - - page 46

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14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 46

30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45

47

123456789

10111213

Icons list

1.2 Control panel

Laser: Value of the exciting line.

Filter: There are 6 neutral filters with the optical densities 0.3, 0.6, 1, 2, 3 and 4.filter [---] = no attenuation (P0), [D0.3] = P0/2, [D0.6] = P0/4, [D1] = P0/10, [D2] = P0/100, [D3] = P0/1000 and [D4] = P0/10000

Hole:Value of the hole aperture.: go to minimum value of the hole: go to maximum value of the hole: Initialize hole, go to minimum value of the hole and go back to the previous position.

Spectr: Position of the spectrograph: go to zero order: go to the diode position: Initialize spectrograph, go to zero order and go back to the previous position.

Time: Recording time (seconds).and number of accumulations

Options:

: 1800g/mm and 300, 600 or 1200 g/mm. Be careful to initialize spectrograph if the gratingis changed.

: Three different objectives can be chosen: ×10, ×50, ×100.

: Enter a name for the next recorded spectrum. The program will add an incremental numberafter the entered name. Be careful to give no more than 8 characters.

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Icon 1 Standard cursor for working

1.3 Description of the panel icons

Icon 1 Standard cursor for working

Allows to come back to the standard cursor after having selected one of the other tools that arepresented by the icons 2 to 11. This tool allows to move the different cursor found in the acqui-sition window.

Icon 2 Peak elimination

This feature removes peaks that we do not want to observe.

Icon 3 Shape correction

This feature permits to modify a spectrum by moving each point of the spectrum.

Icon 4 Zoom

Press the left button of the mouse and draw a rectangle which select the part of the spectrum tozoom.

Icon 5 Horizontal shift

Move the abscissa scale of the spectrum

Icon 6 Vertical shift

Move the ordinate scale of the spectrum

Icon 7 Intensity adjustment

This feature allows to adjust the scale in intensity of a spectrum.

Icon 8 Shift

Move the scale of a spectrum

Icon 9 Manual addition

This option allows the user to add a constant to the active spectrum.This is a manual method, using the mouse:1 Press the mouse left button « +Const »,2 Move the spectrum up and down, then press «OK» to validate.

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Icons list

Icon 10 Manual multiplication

This option allows the user to multiply the active spectrum by a constant.This is a manual method, using the mouse.The procedure is the same as the one for « manual » addition.

Icon 11 Label of the peak

The wavelength or wavenumber of a peak appears if the left button of the mouse is pressed.The label will be deleted by pressing the right button of the mouse.

Icon 12 Move peak maxima

By pressing the left button of the mouse, the label of a peak can be moved precisely. The labelselected is in white, by default it is the last one, but another color can be selected in the window« Bands Parameters » (icon 45 then button Bands)

Icon 13 Fit peak width

By pressing the left button of the mouse, the width of a band can be changed. As for the iconnumber 5, the bandwidth in the window « Bands parameters » can be selected (icon 45 thenbutton bands)

Icon 14 Delete

Delete the active object (e.g. acquired spectrum) in the active window.

Icon 15 Load

Load from the disk. The appropriate previously saved file will automatically be opened.

Icon 16 Save the active object (spectrum)

The active object like an acquired spectrum can be saved on the hard disk.

SPECTRA.

When a spectrum is acquired, it is not saved but remains in the Random Access Memory(RAM) of the computer.If several spectra are acquired, different color dots will be displayed on the right edge of thewindow. Each dot takes the same color as the displayed spectrum and each dot works as a«radio» toggle button. Each time an acquisition is performed a new color button is added.Thefull names of the spectra are indicated in the object menu command.

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The active object like an acquired spectrum can be saved on the hard

To save one of the displayed spectra, activate the « spectrum » window, select the desired spec-trum by pressing the «radio» button, then choose one of the following methods

1- Click on the following icon: then enter the file name, its location and format.

2- From the « file »Main Menu, select the « Save As » option, then enter the file name, itslocation and format.

Before saving, it would be useful to complete the information list about the spectrum (operator,laser power, etc...) by pressing the icon . Some parameters (hole, slit, spectro, grating, time,accum, date) are automatically updated.

LabSpec file formats.

The following is a list of the various file formats that LabSpec supports to save the acquiredspectra or images:

- Compressed Tiff (*.tsf): It is a compressed format, specific to the Labspec software,

- Extended Tiff (*.tsf): It is a specific format of the Labspec software

- Dilor format (*. ms0): This format can be used with the other DILOR softwares.

- Text format (*.txt): This is an ASCII mode format which uses two columns: wave-length/Wavenumber and intensity, without header.

- Spectra Calc format (*.spc), This format is used by « SpectraCalc » and « Grams » process-ing softwares.

IMAGES.

The « save » procedure always saves the content of the active window. To save an image, acti-vate the window that contains all the spectra. If the « single spectrum » window choise is acti-vated, only this spectrum will be saved, and not the whole spectral image.

To save a video image, activate the window that contains the video image.

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Icons list

Image file formats

Extended Tiff (*.tvf)It is a binary format for spectral images, or time kinetic. If a spectral image is saved in this for-mat, the « models » can be saved together with the image. Two images can be added in one file.

Compressed Tiff (*.tvf)It is a compressed format

Text format (*.txt)The data given by this format are the intensities; the wavelength or wavenumber parameter islosen.

Standard Tiff (*.tif)This option should only be used for 2D array of data, not for spectral images.

Color Tiff (*.tif)This format is used to save color Video images.

Icon 17 Cursor normalization

Bring cursor to the center of the active window. Sometimes the cursor is not visible, in this casemove the mouse.

Icon 18 Normalization

Automatic scaling optimization of a spectrum or an image.

Icon 19 Print page

Video images, spectral images or spectra can be inserted and printed on a prepared layout page.There is another way to print the data: transfer them to another «Windows 95/Windows 98»software using the « copy pictures » or « copy data » choices from the « EDIT » menu.

This «Print» icon prints out the objects of the active window

The layout page can be configured with the option «print set up» from the EDIT menu.

Icon 20 Information about object

Information about an acquired spectrum or image. Most of the information is insertedautomatically into this table, enter the name of the sample, the name of the operator, the laserpower, the name of the file and some eventual remarks. Once performed, press « OK » buttonto validate.

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Icon 22 Mathematical processing

Icon 21 Multi-Window automatic spectra gluing

When several regions have been acquired, these regions can be glued with the Multi-WindowOption feature. The result is displayed in a new window and the previous separate regions arenot deleted.

Example:

The figure above gives the example of a 3 windows acquisition, the first one covers 200-500

cm-1, the second one 1200-1800 cm-1 and the third 2300-2700 cm-1.

If the whole spectrum between 200 and 3000 cm-1 must be acquired, select the first line andthe numbers 200 and 3000 in the box « Window limits ».

If the combine feature has not been validated before the acquisition, it is possible to perform itafter an acquisition by pressing the button « COMBINE »; once activated, it will combine allthe spectra of the active window.


These mathematical procedures can be applied to spectra, spectral profiles, spectral images andVideo images.

Available calculations:- Calculate a function of the active spectrum, - Add a constant to a spectrum,- Multiply a spectrum by a constant, - Add, subtract, multiply, divide two spectra,

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Icons list

A:

Constant for addition

This option adds a constant to a spectrum. Enter the constant value in the «+» field, then pressthe button « OK » to validate.

Constant for multiplication

This option adds a constant to the spectrum. First, enter the value of the constant in the firstfield. Then, press the button « OK » to validate.

B: Operations between two spectra

These four buttons allows the user to add, subtract, multiply or divide two spectra.The first spectrum is the current active spectrum but the program will ask for the second spec-trum, if more than two spectra are displayed in the window.

C: Definition of the function

In this field, enter the mathematical functions to activate.The available functions are: +, -, +, /, log(), exp(), sin(), cos(), tan(), atan(), abs(), sqrt(),step(), power xa and any combinations of these.A function of two variables x and y can be also calculated. « x » will refer to the active spec-trum, « y » to a spectrum loaded in the RAM (Random Access Memory). The program willask to define« y » when the button « FUNC » is pressed. If only one spectrum is loaded inmemory « x » and « y » will be the same.

To perform the calculation, press the button « OK ».

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Icon 23 Baseline correction

The spectra baseline can be computed and subtracted by pressing this buttonThe main procedure is to build up « point by point » a baseline, to fit at best to the spectrumand then subtract..

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Icons list

An automatic procedure can also be used for the computation of the baseline, button« AUTO ».

A:Operations for the baseline

Four choices are available:

1- Ins/Del:It permits to select the points for the computation of baseline. Validate the point with the leftbutton of the mouse or deletes it with the right button.

2- +Const:Add a constant to the baseline. Validate this button and move the baseline with the cursor.

3- *Const:Multiply the baseline by a constant. The procedure is the same as the one for +Const.

4- Zoom:It allows to magnify a spectrum zone.

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Icon 24 Correction

B: DeviationChoose the deviation (1% is normally appropriate): that is to say the residual differencebetween the calculated baseline and the optimal one.The automatic calculation of baseline depends on this coefficient.

C: Attachment

A best fitting can be done automatically with this command. It attaches the baselineto the spectrum. It could be useful for the correction of baseline of spectral images.

Icon 24 Correction

When this option is chosen, the following window appears and gives access to some utilitiesfor the correction of spectra and spectral images.

GET: The active spectrum in the « single spectrum » window can be taken as a reference forthe options « normalization », « sub », « add », « div », « mul » and « corr ».The reference spectrum is stored in a « correction window ». This spectrum is removed withthe command « del »

THR

This option is used for the images. For every spectrum of an image, this function activates tozero all the intensity values that are lower than thr(%) of the intensity of the principal peak.This function allows to remove the lowest peaks

Threshold(%)

It is the limit which is expressed in percentage of the value for the threshold for the eliminationof weak peaks.

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Icons list

ZERO

Translate the spectrum or all the spectra until the lower point is brought to zero.

Normalization

Normalization of the spectra to the part of corrector spectrum that is inside the green cursors inthe correction window

MUL, SUB, ADD, DIV

These options allows the user to multiply, subtract, add or divide the corrector spectrum to allthe spectra of a spectral image.

Spectrum correction

Fits at best the corrector to the spectrum by means of a multiplicative factor « k ».The corrector multiplied by the factor « k » is then subtracted from the spectrum.The default fitting is applied to the whole spectrum. If a double cursor is activated, the fitting isdone only inside the cursor.

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Icon 24 Correction

Icon 25 Profile

This window allows the user to glue spectra together in a 3D array, change or insert spectrumin a set of spectra.A profile of a 2D image (CCD image, video image, mapping...) can also be performed

Profile

To profile an image, select the cursor line on this image (if a horizontal line has been chosen,do not forget to check in the « HOR » box).Then press « PROFILE ».A rectangular cursor can be also selected to start profile generation.

Insert

Insert the active spectrum in a set of spectra. With the cursor, choose the position of the spec-trum in the image.

Remove

Remove spectrum from a set of spectra.

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Icons list

Change

Exchanging of spectrum in a set of spectra.

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Icon 24 Correction

Add

Add one spectrum at the end of the set of spectra.In the window « Spectrum » select the first spectrum and press « ADD ». Do the same proce-dure with the others spectra, but don’t forget that the selected spectrum is always added at theend of the set of spectra.

Extract profile

Extract one spectrum from a set of spectra.

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Icons list

Icon 26 Filtering

The Filtering feature can be applied to any data «object» like spectrum, image, spectrumprofile, spectral images ...

Each of these data is a multidimensional cube and each of these has its own specific dimensiondefines as follow:Spectrum: one dimension (wave number),Image: two dimensions (X and Y axis)Spectrum profile: two dimensions (time and wave number)Spectral image: three dimensions (X, Y and wave number)...To perform a filtering at least one dimension must be selected.

The figure below shows the filtering parameters:

Here below is the example of a 3-dimension filtering screen:

Enter here the wei-ght of the elementsof the mask.

Launch filtering

Load customizedfiltering

Data sliding bar

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Icon 24 Correction

Size: Size of the mask (the number of points around the filtered central datum that are involvedin calculations of the filtered value)

Dimension: As explain above, dimension is the parameter on which is applied the filteringprocess. As an example, if you want to filter a spectrum, the dimension will be « F » (wavenumber). For an image, the dimensions available are « F », « X » or « Y ». If a wave numberfiltering is asked for a spectral image, all the spectra will be filtered.

Symmetry: You can choose in the box « symmetry » if the weight of the points of the mask aresymmetrical in respect to the point to be filtered or not.

Type: There are five types of filtering:

- Ordered: Once the mask characteristics has been chosen, the software analyzes the data levels (intensi-ties) located in the mask then replace the central datum level (intensity) by the level determinesusing one of the following options:

- MAX: the maximum level value finded in the mask,- MIN: the minimum level value finded in the mask,- MED: the «middle» level value,- INC: the intermediate value between the maximum value and middle value,- DEC: the intermediate value between the minimum value and the middle

value.

Example: the values of the mask are: 50 10 5 4 8 9 32 68 75 41 3

The first step is to order the point: 3 4 5 8 9 10 32 41 50 68 75The results will be: MAX = 75, MIN = 3, MED = 10, INC = 50, DEC = 5.

- Average :

Choosing this option will substitute the selected datum value (0 in the mask) by the averagevalue calculates from all of the values of the mask. The calculation process uses the followingformula:

yc =

- PeakElm:

The «PeakElm» option modifies the spectrum only if a sharp peak is found. A peak is detectedif its intensity is higher than the enterd threshold value. The level of the threshold must beenterd in the « PEAK » field. A value of one means that the threshold is fixed at 100% of theaverage value calculated from mask values, 2 means 200% etc...

If «I» is the intensity of the point to modify and «Im» the average intensity of the mask points,then the value of «I» is replaced by «Im» if I<Im+I*peak

y

in

i

n

=∑

1

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Icons list

- Linear:

The «Linear» option performs a linear combination of the values of the mask. The coefficients(wi) of the linear combination can be introduced directly in the mask. In this case the value ofyc is:

yc =

Special types of filtering can be saved and loaded again with « LOAD » button.A major example of linear filtering is the derivative. In this case, the mask will contain thecoefficients 1, -1, 0 or also 1, 0, -1 if derivative is performed on 3 pixels.

- Polyn:

Polynomial filtering is only available for one-dimensional filtering. The degree of the polynominterpolates the values in the mask. Enter the degree value in the «ORDER» field.

General: How to perform a filtering ?To perform a filtering on an object, follow the steps:

1- Choose the correct dimension by pressing on the corresponding button.2- Select the type of filtering and the mask size.3- Press « FILTR » to perform the filtering.

w yi

wi

i i

n

i

n

×=

=

∑

∑1

1

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Icon 24 Correction

Icon 27 Fourier transform (filtering)

This option allows the calculation of the Inverse Fourier Transform of a spectrum.It can also be used for images.By applying mathematical functions to the interferogram, it is possible to correct some defaultsof the spectrum

Dimension: Choose the dimension where you want to calculate the IFT (inverse Fourier trans-form) (Freq. for a spectrum, X or Y for an image)

Function: Mathematical filtering: exp (b*t), trafic, Low band

Apodisation: Mathematical filtering (apodisation): 1-(t/T), 1-(t/T)2, cos (t),

Remark: the parameters of the different correction equations can be easily modified by adjust-ing graphically their curves with the mouse

DFT: After a modification of the interferogram this icon allows you to calculate the Fouriertransform and modify the spectrum of the active window

IFT: Calculation of the inverse Fourier transform

Z%: Increase or decrease the abscissa axis of the interferogram

DFT : Automatic calculation of the Fourier transform

IFT : Continuous calculation of the inverse Fourier transform.

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Icons list

Icon 28 Peak fitting

The Peak Fitting feature detects the peaks using different shaped bands, like gaussian/lorentz-ian or even « custom » ones.

There are two methods of detection of the peak:

1. AUTO: The automatic detection of peaks is obtained by pressing the button « SEARCH ».You musttake care of the position of « HEIGHT » an « NEIGHBOUR » scrolling bars.

HEIGHT/NEIGHBOUR: A peak is detected only if it is a local maximum and if its intensityis higher than a threshold. The threshold is fixed by « HEIGHT » scrolling bar. The width ofthe local region is defined by « NEIGHBOR » scrolling bar. If a spectrum extends from fre-quency « f1 » to frequency « f2 » with a maximum « MAX », a minimum « MIN » and« bar% » is the position of the cursor bar,

then the threshold is:

THRESH = MIN + (MAX-MIN)*bar%

and the width of the « local » region is:

ZONE = (f1-f2)*bar%

2. Icon n° 11

CLEAR: Delete all the selected peaks.

The « label » button allows you to put the label of the different peaks by using the icon .

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Icon 24 Correction

The « sum » button displays the sum of all bands, i.e. the fitting.

The « shapes » button displays the shape of each single band.

With the button « attach », the label can be fixed to the related peak.

BANDS: In the window « BANDS » we have the result of the peak fitting procedure. (This listof results can be saved with the icon SAVE)

⇒ For the images, if you want to have the fitting of the position, the surface, the amplitude orthe width at half maximum, in the « Bands window », activate the buttons « p », « s », « a » or« w ».

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FUNCTION:

There are four possibilities:

- Gaussian/Lorentzian

y = a*(g*exp(-((x-p)*(x-p))/(w*w))+(1-g)/(1+4*((x-p)*(x-p))/(w*w))

- Gaussian

y = a*exp(-((x-p)*(x-p))/(w*w))

- Lorentzian

y = a/(1+4*((x-p)*(x-p))/(w*w))

- Line

y = c*y + a

VAR: Select the variables of the different functions.

In this window, select the variables of the different functions.You can choose the maximum and the minimum of each variable. Or you can fix one of theseparameters.NOTICE: if you fix one parameter in this window, for example « p », this variable will be fixedfor all the bands that you want to fit, so if you want to fix the parameter « p » for just one band,you have to do it in the window « BANDS » by checking in on the desired parameter)

- The variable « p » is the position of the band.- The variable « a » is the amplitude of the band.

- The variable « w » is the width at half maximum. - The variable « s » is the surface of the band.- The variable « g » is the Gaussian/Lorentzian ratio.

APPROX: It calculates the theoretical shape of each selected peak. It permits to correctly ini-tialize the peak fitting parameters

TIME: Maximum time for calculation

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Icon 29 Integral calculation

Deviation factor

The optimization of the fitting parameters is done iteratively with the method of maximum gra-dient. In each step (j) the deviation of the interpolation from the real spectrum is calculatedsumming over all spectral pixels from f1 to f2.

d(j) = (If-Sf)*(If-Sf)

Where « If » is the intensity at frequency « f » and « Sf » is the value obtained at frequency« f » by the peak fitting.Deviation is defined so that if « deviation » > |d(j)-d(j-1)|/d(j), the calculation is stopped as itis near enough to the solution.

Icon 29 Integral calculation

To calculate an integral under a band, zoom the band of interest. Select the green cursor, posi-tion the « first » and the « second » cursor with the mouse. Then press the icon

Icon 30: Palette window

It allows you to choose the colors for drawing of plane and 3D representationsof images.

- grey: Select a grey level palette.

- FALSE: Select a 16 false colors palette

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- TRUE:Select a 3 (red, green, blue) colors palette, each color with intensity levels. This drawing style is only used when superimposing two images.

- OTHERSPalettes are the same as grey but the black and the white are substituted by other fundamentalcolors.

You can also adjust the contrast and the brightness of your image

Icon 31 Zoom

Zoom in either X, Y, wave number and intensity dimensions. For images and spectra.

Extract

If you are just interested by a part of a spectrum or if you want to limit the wave numberdomain of a spectral image, you can use this button:First, you have to select the part of the spectrum (or the spectral image into « all spectra

window »), for that you have to make a zoom by using the icon .Then press the button « Extr »: on the screen you will have the selected part.

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Icon 31 Zoom

Icon 32: Spectral mapping procedure

This window describes the algorithms which are used for the mapping. It gives us some spec-tral information:

- Description of the cursors (Red, Green, Blue) There are 3 types of cursors (green, blue and red), there are in «all spectra» window. You can set them around the interested bands and if the options « red », « green » and « blue » are active, it gives you the mapping of the different cursors.

- Green/blue It is the ratio between the contents of green and blue cursor of the window «all spectra», either in mode «Raman» and «Fluo».

-Decomposition

It is the procedure used to make some « spectra models » (this option is in « mathematicaltreatments »)In this procedure, each spectrum of a spectral image is fitted with a sum of« model » spectra.The relative importance of each « model » in the fitting can be mapped.

-Extract procedure

This procedure is used to have the spectrum in a « single spectrum » window.

Deconvolution

It gives you the percentage for the contribution of each of the « model » components in the dif-ferent points of the mapping.

Summa

It gives you the error made by the calculation of the% of the different « model » components.

Raman/Fluo

Using of baseline correction for cursors (red, green, blue) operation.«Raman» mode discards baseline contributions while «Fluo» mode calculates the integral fromzero.

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Icon 33 Model

It is the decomposition of each original spectrum of an image as a sum of « model » spectra.

1. Choose the spectra that will become « MODELS »: they can be already saved on thedisk, or they can be particular spectra that you can extract and save from your spectralimage.

2. If the spectrum is a particular one extracted from the image, save it.

3. Do the same thing with the other interesting spectra.

4. Load all the selected spectra and put them overlapped in a window (for that use theoption « behaviour » of the Format menu command).

5. Activate the first selected spectrum and press the button GET. Do the same for all theselected spectra: a window is then created with all the « model spectra ».

6. In the « single spectrum » window, there are:

- The « models » with relative intensity.- The experimental spectrum in particular point of the image.- The result of the fitting.

You will see in the mapping window additional pictures that correspond to each one of the« model ».You can overlapped them to see the relative contribution of each of the spectra in particularpoint of the image (for that use the option « behaviour » of the Format menu command).

7. You can save a whole spectral image with the « model » compounds choosing the « tvf »format. When you will load this image, the decomposition will automatically appear.

Icon 34 Video image

Video image grabbing. The video image is frozen by pressing the icon « STOP » (n°46). Thetype of cursor (buttons at the right lower edge of the window) indicates the type of object youchoose to record in imaging mode.

Icon 35 Spectrum adjustment

If you want to maximize the signal and adjust experimental conditions you can use this icon This option is a spectrum adjustment (continuous recording of spectra, the new spectrumrefreshes the old one and so on), so it helps you in maximizing the signal and in adjustingexperimental conditions.

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Icon 37 CCD image adjustment

Icon 36 Spectrum recording

This option permits to start spectrum recording without moving table in the point indicated bythe point into the video image. It is the simplest procedure to record a spectrum. With this icon you can also do a multi window recording of spectra by choosing the parametersin the window « Spectral windows parameters » (icon 21)

Icon 37 CCD image adjustment

This option permits to record a CCD image. The procedure is the same as the one for adjusting a spectrum (refer to the help

of the icon ). But to record the CCD image you have to press the icon . This option is useful to check the position of the spectrum on the chip in orderto select the good « binning »: You can see precisely the spectral domain of interest.

Icon 38 Spectral images recording

If you have an XY motorized table, or if you want to do a time kinetic recording, you can selectsome points on the video image to have their spectra.

This procedure is used for recording spectral image or line scanning recording, time kineticsand also spectra positioning the XY table in the video image, instead of the icon thatrecords the spectra without moving table.To activate this icon you first have to record a video image, and you have to choose the type ofcursor that will define the area to analyze.Meaning of these different cursors:

- Point cursor : You select or delete a point with the right button of the mouse,you move a point with the left button of the mouse

- Inclined line : N spectra are recorded point by point along the line.

- Horizontal line : N spectra are recorded with Laser scanning along the line orpoint by point.

- Rectangle : An array of N*M spectra is recorded inside the rectangle withlaser scanning or point by point.

- Elliptic and polygon : You can use these cursors if you are just interesting by a part ofthe sample. It is a point by point recording.

After the acquisition three windows will be opened

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« Single spectrum window »

Three cursors are available and are selected with the buttons at the right lower corner:- A red single cursor for the wavelength and the intensity readout.- A double green cursor for the readout of the width of a band.- A yellow cursor composed of a continuous line and two dotted lines. The continuous lineautomatically positions to the maximum of the band in the neighborhood of the mouse arrowand the two dotted lines position at the half maximum of the band.

« All spectra window »

This is the full representation of a spectral image. All the spectra that compose the spectralimage are displayed overlapped. In this window, three cursors allow the selection of the bandsfor the selective spectral mapping.

« Spectral mapping window »

This window contains the mapping of some spectral features. For example: mapping of a band,ratio between two bands, spectral models decomposition etc...

Icon 39 Data size

This option permits to select the parameters of spectral images (Xsize, Ysize, Zsize, number ofspectral points). If you want to do a time Kinetic recording, the choice of the time interval isdone in this window.

X

In the box size, you can select the number of points (spectra) that you want to record alongeach line. It is the «binning» (cf. Spectral) in spatial dimension (the short one).Or if you want you can choose the step between two measurements in the box Step&Binning.

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Icon 39 Data size

Y

In this box you can select the number of lines of a spectral image.

Spectral.

This box contains the number of spectral points read out of the detector. The number of data points along the wave number direction can be less than the number of pix-els because you can choose to readout some pixels together. This technique takes the name of«binning». If two pixels are «binned» together, their intensities are summed up, thus you gainin S/N, but, of course, you loose spectral resolution.The binning of the pixels of the CCD can be useful if you are just interesting in mapping the intensity of one spectral band.

For example, if you have a detector with 1024 pixels along the spectral direction, you canchoose to read all the pixels and then the spectrum will be composed of 1024 points.If you don't need spectral resolution, you can choose to work, for example, with 200 datapoints: the signal from 5 pixels is summed up and gives rise to only one data point. Of course,1024/200 gives not an integer result and some pixels of the CCD will not be displayed.

Time

There are two types of boxes, the first one is the number of measurements required and the sec-ond one is the time interval between them.

Be careful, because the acquisition time is included in the interval of time between two mea-surements.

Z

As for the «time» dimension there are two boxes, in the first one you introduce the number ofmeasures and in the second box you introduce the interval between the measures.

This option can be automatic with a piezo, if you have no piezo, the program stops, asks you tomove the focus and start again.

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But you can do more...

Time

There are two types of boxes, In the first one you select the beginning and the end of the analy-sis. In the second one you choose the time interval between them.

The choice of the time interval between the measurements is introduced as a functionof the number of the measure « t ». A value: 30 * t will produce a spectrum each 30 seconds, 60 * t each minute.You can introduce more complex timing like log (a*t), exp (a*t) or a*t*t where « a » is a con-stant.

Depth

As for the «time» dimension there are two boxes, in the first one you introduce the beginningand the end of the analysis and in the second box you introduce the interval between the mea-sures which is a function of the number of the measure «z».

This option can be automatic with a piezo, if you have no piezo, the program stops, asks you tomove the focus and start again.

Y

You introduce the beginning and the end of the analysis. And you can choose to have a con-stant size or a constant step between the measurements

X

You introduce the beginning and the end of the analysis. And you can choose to have a con-stant size or a constant step between the measurements

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Icon 39 Data size

Icon 40 : Acquisition parameters

Spike removing:

This option is active only when there is more than one accumulation; it is recommended to give3 or more accumulations to have a good spike removing.

X scanning:

Select laser scanning or point by point XY table imaging.Of course in mode « Scanner » the table will be used to scan the Y direction while the laserwill scan the X direction.

Scanning area: Cursor or manual

- Cursor: The size of the image is selected with the cursor in the video image.

- Manual:The upper left corner and the right bottom corner are selected moving the XY table.If you choose this option, the software will ask you to move the table in the appropriate posi-tion and validate.

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Refresh time

The PC can not record and display the data of a spectral image at the same time.It must stop the recording in order to display the collected data, you can choose to stop period-ically the recording and display the data by selecting a refresh time.

For the images, to reduce the total time of acquisition procedure, it is better to choose a refreshtime equal or higher than 10 minutes.For example, if the refresh time is 300, it means that each 300 seconds, the recording of a spectral image will stop, the display of data is refreshed on the screen, and the recording startagain.

Autofocus - Autofocus offset

This system is an autofocusing of the microscope stage to enable systematic measurements ona device at different operating points. You can choose to add an offset to your autofocus, forexample if you want to take a measurement at 2 µm in depth choose an offset of 2

This option is active if you choose in the file Hardware.dat: « PresentAutofocus = 2 »

Confocal value

You can choose to have :

- The intensity in function of the position of the point, if you have chosen “confocal value =Intens.”or- The z value where the intensity of the signal is maximum, if you have chosen “confocal value= Depth”

Icon 41 Detector parameters

This option permits to select the stripe of the CCD used for single spectrum readout, or theregion of the CCD used in image mode.

The choice of the region of CCD used for measure allows you to limit the spectral domain ofinterest and to avoid to read regions without signal that would only increase the readout noise.

Usually you have not to change these parameters (settled at DILOR) unless you use the fiberoptic entrance. In this case the interesting detector area will be substantially larger than the oneobtained under microscope.

(In this picture the example is done with a 256*1024 detector).

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Icon 42 Morphological images

When you record an image of the CCD, you can see the area where the bands are. So, withthese different parameters, you can select this area. You are allowed to limit the wave numberdomain and the spatial domain.

Icon 42 Morphological images

Clicking on this icon will display a morphological parameters window. These parameters willallow you to get morphological images.To have access to this feature, you have to take a Video image and choose the rectangular cur-sor in this image.The three first lines allows you to take morphological images in function of the depth.In the «First Z» and «Last Z» fields, enter the beginning and the end of your depth profile (e.g.:-10 and 10).In the field «Size Z», enter the step between two measurements in depth.«Size X» and «Size Y» are the number of points you want to analyze in the X and Y direction.«Intensity» is the number of counts read out from the PMT during the time defined in the«Time» field.«Repeat» is the number of total measurement cycles.«Single image» option allows you to save all of the morphological images depth profile on afile.To start measurement, press on the «START» button.

Icon 43 Calibration

This window is used to calibrate the moving of the XY table and the amplitude of the scanner.

1. First, you have to select the appropriate objective and record a video image of the samplewith the laser spot. This one must be attenuated in order to see its position well. The sam-ple should have some sharp features (corners, dots, dust particles etc...).

2. Press the button .

3. Choose the cursor « point » into the video image, then position the small rectangle onthe laser spot. Press « SET » on the box « Centr (x,y) » of the « Scale window ». Thecalibration of the laser is done, and when you will record a new video image, a color dot

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will indicate the laser position.

4. Now measure, with the XY table, the distance in X and Y between two details of thesample (the values read out from the table display are ten micrometers. So a read out like675231.2 means 67523.12 micrometers).

5. Choose the rectangular cursor into the video image.

6. Position the two corners of the rectangular cursor onto the selected details of the sample,then introduce in the box « Table (dx,dy) » the dimensions of the rectangle as measuredwith the XY table. Press « SET » on this box.The equivalence between the video camera pixels and the micrometers are now settled.

You can check the calibration of the table if you record a spectrum in a selected point ofthe video image.

You have to do this calibration for each objective

Now calibrate the scanner:

- Put the laser scanning mode.

- Select the OD 4 filter in order to attenuate the laser

- Select the horizontal line cursor on the video image with the length approximately 10% less than the scanned line and center it on the laser spot.

- Open the window « SCALE ». Press « SET » on the box « Scanner (l,r) ».

You can check the calibration of the scanner doing an image of the sample.If the calibration is not correct, select the cursor line shorter or longer and press again « SET »on the box « Scanner (l,r) ».

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Icon 43 Calibration

Icon 44 Configuration

To program some configurations in function of the system.Follow the different steps below:

1- Choose the type of user.2- Press the ADD button3- Select all the parameters in the window « Parameters »

To use one of your programmed configurations, in the window « configuration » select thedesired configuration then press « set », the software will automatically change all the parame-ters.

Tip: to delete a configuration, select it and press DEL.

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Icon 45 Scheme

This icon permits you to control all the functions of your system:

Icon 46 Stop

Stop of spectra recording. Freeze the video image after pressing the Video button (icon n°34)

Icon 47 Different types of cursors

Three cursors are available and are selected with the buttons at the right lower corner:- A red single cursor for the wavelength and the intensity readout.- A double green cursor for the readout of the width of a band.- A yellow cursor composed of a continuous line and two dotted lines. The continuous line automatically positions to the maximum of the band in the neighborhood of the mouse arrow and the two dotted lines position at the half maximum of the band.

control:

Differentcameras (A)

Laser shutter(B)

Filters wheel(C)

White lights(D)

Different secu-rity switches

(E)

A

BC

D

ED

change:

Acquisitiontime

Spectrographposition

Grating

Hole aperture

Exciting line

SpectrumAcquisition

ImageAcquisition

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1.4 Menu Bar Description

The menu bar is located in the upper left hand corner of the screen.

The « File » menu commands:

Open: To open a previously saved spectra for display.Save: Allows a spectra to be saved for later retrieval.Split: Allows to decompose a spectral image as a set of spectra (you can use

this option before or after the recording of the image, when you choosethis option, the software asks you a name for the spectra and will incre-ment it by a number for each spectrum)

Delete: Delete the active object of the active window.Print: It allows the output to a printerPrinter set-up: Printer parameters selection (see the figure below)Exit: Quit and close the LabSpec application.

configuration of the print page.You can choose to have a colored or a black and white printingYou can choose the orientation of your print page...

To print a spectrum, select the spectrum to print (it must be alone in the window, if not it willprint all the object of the active window) then to insert it in your print page press the icon .To introduce the parameters in your print page, press the icon , select the parameters andthen press OK.

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The « Edit » menu commands:

Copy Text: Copy the text or the parameters that have been selected in the print page.Copy picture: Copy the contents of the active windowCopy data: Copy the data of the active objectPaste Text: Allows the ability to paste text in the print pagePaste picture: Allows the ability to paste a picture in the print pagePaste Data: Allows the ability to paste a spectrum in the « Spectrum window »

This function can be useful to keep an object before correction opera-tions, if these modifications are not used we just have to recall the origi-nal object with this option.

The « Format » menu commands:

VIEW

DISPLAY:

: Spectra in continuous line

: Spectra in dotted line

: Spectra represented in full surface

: Plane surface

: Contour line

: 3D representation

BEHAVIOUR

For the spectra:

: Active spectrum alone.

: All the spectra overlapped.

: All spectra in separate smaller windows.

: All the spectra in the different points of the spectral image.

For the images :

: Active image alone.

: All the images overlapped in the true color palette.

: All the images in separate smaller windows.

: All slice images.

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SUPERPOSITION

For the spectra:

: Only limited spectra are shown (min. and max.) with filled space between them.

: All the spectra are overlapped in the window (it could take long time when the PC dis-plays the collected data)

: Only a part of the spectra is shown to make displaying time less than 1-3 seconds.

: 25% of the spectra are shown.

For the images:

: Unsmoothed display.

: Smooth along lines.

: Smooth along columns.

: Smooth.

IMPOSITION

These icons correspond to the different forms of overlapping the recorded spectra or spectralimages on the video images.

a : Off mode.b : It gives you the points where spectra were recorded on the video image.c : It is the same as b but it also displays the names of the spectra.d : It gives the rectangle where spectral images are recorded. e : The same as d but it also gives the names.f : It gives you the superimposition of spectral images on the video image.

COLORS

You are allowed to put or eliminate a coordinate axis (wave number, intensity, X or Y) or put a

label on an axis (for example: wavenumber (cm-1): select with an « X » the axes or labels thatyou need.The expressions for the labels can be changedYou can also change the color of a spectra or of the background

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3D MODE:

You can represent an array of spectra (or a 2D image) like a3D profile.You are allowed to choose the drawing style. You come back

to the original display by pressing the icon of the

« display » option (menu « VIEW »

This option can be used after « 3D mode option ». It permitsyou to see the 3D profile according to different angles.

SCALE

- Comm: Common scale. It puts the maxima of the spectra at the same scale.- Incr: Incremental scale. It automatically changes the scale when the limits are expanded. - Freeze : Freeze scale. It freezes the auto-scaling.- Auto: Auto-scale.

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The « object » menu command:

Full list of the objects contained in a window (for example, list of the spectra contained in a spectral window).

The « Option » menu commands:

Multi: This procedure selects all the objects that are on the screen and permits to make an operation to all these objects. For example, you have three spectra on your window, and you want to add the same constant to all these spectra, activate the option « multi », the addition will be done on the three spectra. If the option « multi » is not active, you will have to do this operation for each spectrum.

nm-cm-1: choose between nm and cm-1 units.

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Auto save:

1. The « Auto save » option

1.1 You can choose the directory where the spectra will be recorded:

BE CAREFUL TO HAVE NO MORE THAN 8 CHARACTERS

1.2 You can choose the files where the spectra will be recorded:

BE CAREFUL TO HAVE NO MORE THAN 8 CHARACTERS.

1.3 You can choose the format of the files:

You can choose a name

You can choose to incrementthe name by the day, the month and the year.

You can choose a name

You can choose to incrementthe name by number, hour and minutesof the acquisition.

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2. You have two choices:

2.1 You can choose to work with the « Auto Save » and « Auto Repeat » options:For that you choose the delay between two acquisitions (ex: 10 seconds). When you press theicon the software takes an acquisition and record it each 10 seconds.

2.2 You can choose to work only with the « Auto Save » option:

- When you press the icon the software takes an acquisition, stop and record it.

- When you press the icon (that you use to do spectral imaging, time scanning...) the soft-ware take a spectral image and at the end of the acquisition will record it.

BE CAREFUL TO CHOOSE THE « TSF » FORMAT FOR THE SPECTRAL IMAGES.

The « Window » menu commands

Reorganize: This option permits to re-organize the windows. And permits to selectone of the windows that are on the screen (of course, you can select awindow with the mouse).

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1.5 Tips section

This section develops some specific practical procedures.

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How to acquire a spectrum?

1. Select the appropriate grating:- 1800 g/mm for a high resolution - 300 or 600 g/mm to make a one shot spectrum- 1200 g/mm which is infrared optimized. (Version only for NIR)

2. Select the correct grating setting in the software. If you change the grating, do not forgetto go to the zero order position ( in the software). This will give the correct calibrationfor the particular grating you will use.

3. Focus the laser on the sample. This can be done through the video image .

4. Set the spectrograph at the right position.

5. Select the confocal hole and the slit aperture.

6. Select the acquisition time and the number of accumulations (this will improve the sig-nal/noise ratio)

Then you have 2 possibilities:

- The icon : is a spectrum adjustment, so it can help you in maximizing the signal (the newspectrum refreshes the old one and so on...). No repeated accumulations or extended spectralranges are acquired.

If you don’t save the spectrum that you have recording with the icon , it will beautomatically deleted as soon as you will record a new spectrum.

- If you press the icon it will make a spectrum accumulation and stop.

Tip1: How to acquire a spectrum?

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How to record spectra at selected points?

For LabRam and LabRam Infinity Systems configured with the motorizedXY Stage

With the motorized XY stage.

1. Focus the laser on the sample.

2. Take a video image

- Press the icon - To freeze the video image press the icon

3. Choice of the cursor.

You can select different points to analyze on the sample, for that, choose the cursor « point »and position a small rectangle on each point that you want to analyze with the right button ofthe mouse. If you want to move the point, drag the box by holding down the left mouse button,if you want to delete a point, press the right button.

4. Choice of the grating

Select between 1800 and 600, 300 or 1200 grating options. If you change the grating do notforget to make a zero order.

5. Select the binning on the box Spectral of the icon . (cf. index)

6. Select the hole aperture and the slit apertures (for the LabRam System). Select the timeof exposure and the number of accumulations « Accum ».

7. To start the recording, press the icon .

Tip2: How to record spectra at selected points?

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Confocal mapping

For LabRam and LabRam Infinity Systems configured with the motorizedXY Stage

1. Record a video image (icon ) and freeze it (icon )

2. Selection of the cursora- Inclined line b- Horizontal line c- rectangled- Elliptic e- Polygon

This define the area to be analyzed.

3. Select the parameters for imaging: icon You can select the binning (box Spectral)The number of points (spectra) that you want to record along each line (box X)The number of lines (box Y)

Of course if you have chosen the horizontal line cursor the box Y is not active.

4. Selection of the acquisition parameters. (icon )In the box X Scanning: choose « table »Choose the refresh time of the computer display

5. Select the hole aperture and the slit apertures (for the LabRam System) and the timeof exposure.

6. To start the recording press the icon

Tip3: Confocal mapping

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Spectral images using a line scan illumination of the sample

FOR LABRAM CONFIGURED WITH LINESCAN

In the LabRam:- The stem 2 is pushed.- Replace the pinhole H1 by the field lens.

On the electronic control box panel:Switch on the scanner.

On the software:

1. Focus the laser on the sample.

2. Record a video image and freeze it with the icon

3. Choice of the cursor:There are two possibilities: Horizontal line and rectangle .

4. Select the parameters of imaging: icon - Select the binning- The number of points that you want to record along each lines (box X)- The number of lines (box Y), of course, if you have chosen the line cursor, the box Y is notactive.

5. Select the acquisition parameters: (icon )In the box « Scanning device » choose « Scanner »Select the refresh time.

6. Select the slit and the hole apertures and the exposure time.

7. To start the recording press the icon

NOTE: Do not forget to switch off the scanner, and replace the pinhole H1 once you have fin-ished your linescan measurements.

Tip4: Spectral images using a line scan illumination of the sample

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Time kinetic recording

This option enables you to take a series of spectra or spectral images as a function of time.You follow the same procedure as the one to record point by point spectra or spectral images.

But you also have to choose:

- In the window « Data size » (icon ): select the parameter « time »There are two types of boxes, the first one is the number of measures and the second one is thetime interval between them.

Please refer to the pages

Be careful, because the acquisition time is included in the interval of time between two mea-sures.

Press the icon to start the acquisition

Tip5: Time kinetic recording

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Z scanning

1. Focus the laser on the sample,

2. Take a video image and freeze it with the icon ,

3. Choose the cursor,

4. Select the time of exposure,

5. Select the binning (icon ),

6. In the window « Data size », you select the parameters of the Z scanning.Please refer to the «Index section»

7. Press the icon to start the acquisition

Tip6: Z scanning

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Baseline correction

The main procedure is to build up point by point a baseline, to fit at best with the spectrum andthen subtract.

If you have to subtract the baseline of a spectrum, activate this spectrum.If you have to subtract the baseline of an image, activate the window « spectral-image ».

1. Verify that the option Ins/Del is active (in the box « operations »)

2. Select the type for interpolation: it can be linear or polynomial (in the box « type »). Forthe polynomial interpolation, you can also choose the degree of the polynom (in the box« degree »).

3. You can select with the mouse in the spectrum window (validate: left button, delete: rightbutton) the points for the baseline computation

Tip: (for the images, when you press the icon , a window that contains an average spec-trum of the image is opened automatically).

4. Activate the option « attachment », to fit at best the baseline to the spectrum.

Tip: An automatic procedure can also be used for the computation of baseline: button« Auto »

5. Press the button « SUB ».

NB: The baseline can be saved as a spectrum: for that press the button «CONV »

Tip7: Baseline correction

LabSpec Software 62


Peak Fitting

To obtain a good Gaussian/Lorentzian fitting, you should prepare the spectrum from spikes.

Tip: Correction of the spikes from an image:The correction is done for each spectrum. If a spectrum of an image presents a spike, removethis spike in the window « Single Spectrum », then press (on this window) the button « R », itwill automatically remove this spike from the image.

1. Zoom and extract (button « extract » of the icon ) the interesting part of the spectrum(or of the spectral image).

2. Press the icon

3. Select the function (button « FUNC »), you can choose particular function for each bandof the spectrum.

4. Select the position of maxima: - Press the icon - The left button add a peak, the right one delete a peak already introduced. (If you want to delete all the selected peaks, press the button « Clear »).

5. Press the button « APPROX » to initialize the parameters

6. Press the button « FIT » to start the peak fitting procedure.

For the images, the procedure is the same as the one for the spectra, but the selection of max-ima is done in the window « Spectral image » and you have to give a long time for the proce-dure of peak fitting (more than 5 minutes).

The results of the peak fitting procedure are in the window « Bands ». You can save theseresults when you press the button « save » (the files format is *.bnd).If you want to print the board that contains the results of the peak fitting, open the window« Bands Parameters », do a copy text, open the window « Print page » (option page « setup » of the « edit » menu) and to insert this board, choose the option « pastetext » of the main menu.

Moreover, for the images, you can have the mapping of the position, the surface, the amplitude,the width at half maximum of the band: In the window « Band parameters » press the buttons p(for the position), a (for the amplitude), w, s or g the results are in the window « Spectralmapping ».

Tip8: Peak fitting

LabSpec Software 63

Icons list

1333.3

1000

800

600

400

200

0

1320 1325 1330 1335 1340 1345

Wavenumbers (cm-1)

10

20

30

40

0 10 20 30 40

Length X (µm)

Video image

10

20

30

40

0 10 20 30 40

Length X (µm)

10

20

30

40

0 10 20 30 40

L th X ( )

Mapping of the full width at halfmaximum. White color correspondsto a 9 cm-1 FWHM and the blackone to a 4 cm-1 FWHM

Mapping of the position of the bandWhite color corresponds to the1336 position, the black one to the

1332 cm-1 position

10

20

30

40

0 10 20 30 40

Length X (µm)

Overlapped of the FWHM and posi-tion mapping. The shift of the bandgoes with an enlarging of this band

LabSpec Software 64


Models

It is the decomposition of each original spectrum of an image as a sum of « model » spectra.

1. Choose the spectra that will become « MODELS », they can be already saved on thedisk, or they can be particular spectra that you can extract and save from your spectralimage.

2. If the spectrum is a particular one extracted from the image, save it.

3. Do the same thing with the other interesting spectra.

4. Load all the selected spectra and put them overlapped in a window (for that use theoption « Behavior » of the menu « view » of the FORMAT menu).

5. Activate the first selected spectrum and press the button get. Do the same for all theselected spectra: a window is then created with all the « model spectra ».

6. In the « single spectrum » window, there are:

- The « models » with relative intensity.- The experimental spectrum in particular point of the image.- The result of the fitting.

You will see in the mapping window additional pictures that correspond to each one of the« model ».You can overlapped them to see the relative contribution of each of the spectra in a particularpoint of the image (for that use the option « Behavior » of the menu « view » of the FORMATmenu

7. You can save a whole spectral image with the « model » compounds choosing the « tvf »format. When you will load this image, the decomposition will automatically appear.

Tip9: Models

LabSpec Software 65

Icons list

With the help of the mapping, we can distinguish three different spectra which are:

We can have the mapping of the relative contribution of each compounds in the analyzedsample:

20

40

60

80

100

Length Y (µm)

0 20 40 60 80 100 120 140

Length X (µm)

video image of the sample:The spectra have been takeninside the rectangle.

0.8

0.6

0.4

0.2

0.0

800 900 1000 1100 1200 1300 1400

0.8

0.6

0.4

0.2

0.0

800 900 1000 1100 1200 1300 1400

Red compound Green compound

0.8

0.6

0.4

0.2

0.0

800 900 1000 1100 1200 1300 1400

Blue compound

20

40

60

80

Length Y (µm)

20 30 40 50 60 70

Length X (µm)

Greencompound

Blue compoundRed compound

LabSpec Software 66

1.6 LabSpec for the T64000 System

This chapter will only detail the LabSpec specific features related to the T64000 System.

What is the T64000 System?The T64000 System is the most powerful, fully computerized, triple spectrograph/scan-ning spectrometer system ever produced. Unlike the current generation of Raman spec-trographs in which spectrometer control and data acquisition are independent functions,requiring different software and hardware, the T64000 System has an integrated softwareand hardware package which combines all functions in a simplified, easy-to-use fashion.The package contains a large number of security and validity tests, which are only possi-ble with the complete integration of all functions. Conceived initially for the demandingperformance of Raman spectroscopy, the T64000 is also superbly adapted to most otherspectroscopic techniques. As the software allows the T64000 System to be used in wave-length and wavenumber units, other spectroscopic techniques such as fluorescence, lumi-nescence and absorption/transmission can also be easily performed on both macro andmicro samples. The ability to use the T64000 as a single or triple (with subtractive oradditive premonochromator stages) spectrometer with either standard scanning or multi-channel acquisition of data, permits the user to optimize the spectrometer to the spectraland sampling requirements. This flexibility is achieved without having to disturb thesample under investigation and is completely automated.For industrial research or analytical laboratories the T64000 System is the safest, easiestto operate, and most flexible spectro-analytical system available.

Why a dedicated User Guide Chapter?The LabSpec software must be installed and setup correctly to manage up to 14 Steppermotors, 15 DC motors and 4 electromagnets.

T64000 System and Labspec Software installationThe T64000 System and the LabSpec software must be installed and started up by Dilor-Jobin Yvon-Spex or an approved representative.Notice: The T64000 System starting recommendations are described in the T64000 UserManual; Part Number: 31 087 018.

What are the new features that handle the LabSpec?The LabSpec Software now handles the features related to the T64000 Triple RamanScanning/Spectrograph spectrometer. The new features only concern the additionaldevices mounted on the System and then do not change the use of the existing LabSpecsoftware.

LabSpec Software 67

T64000 Configuration Setup

The figure above shows the icon which allows the user to setup the T64000 configuration. Thefollowing screen will then be displayed.

First Stage Entrance The default choice is Axial especially if the Microscope or the Macro-sample compartment is used. The Lateral choice is an option for spe-cific needs required by the user.

Analysis mode The Micro mode concerns the use of the Microanalysis System, theMacro mode concerns the use of the Macroanalysis System. Both areoptions. the selected option must be present on the system.

Click here to setup configuration

LabSpec Software 68

Spectrograph The T64000 is a triple stage spectrometer. Therefore, the last stage canbe used alone (Single) for some specific applications.

Premonochromator The Premonochromator includes the first two stages. By default thesestages are configured as subtractive. An additive option can be added.See the hardware user manual for details.

Second/Third Stage The link on the exit of the premonochromator and the entrance of thethird stage can be direct (default) or external (option). That means thatthe exit beam can be adapted for a specific application (e.g. Fluores-cence option) then analyzed with the third stage.

Detection System Select the detector you want to use: Multichannel (CCD) orMonochannel (Single channel PMT or IR detectors).

Laser Shutter The laser shutter is located before the Microanalysis or MacroanalysisSystem. Usually, to prepare an analysis session, the laser beam must bepresent in the microscope or in the macro-sample compartment. Withsome type of samples, the laser beam can modify or destroy a sample.For these reasons, two choices are available: Always open maintainsthe shutter off and Open during acquisition opens this shutter only dur-ing the acquisition (adapted for laser sensitive samples).

Initialization Clicking on this button will reinitialize all the commutations listed inthis screen plus the grating turret.

OK Validate the selected choices.

LabSpec Software 69

Monochannel/Multichannel SetupThe previous chapter explains how to select the Monochannel or Multichannel detector for theSystem which includes both of these detectors.Default, on all T64000 Systems, the Monochannel (PMT) detector is mounted on the thirdstage axial exit and the Multichannel (CCD) detector is mounted on the top exit. Once the Lab-Spec software is running, the bottom left screen shows the following setup screen, specificallydedicated to the T64000 System. The use of the displayed parameters is slightly differentdepending on the chosen mode: Monochannel or Multichannel.

Premonochromator setup Spectrometer setup

Single moving

Double moving

Wavelength calibration screen

Close the current slitList of slits

Slit calibrationMaximal open slit

o

LabSpec Software 70

How to setup a Monochannel PMT (PhotoMultiplier Tube) detector?

On the figure page 70, the bottom left bar includes two new sections fitted for the T64000 Sys-tem: the Premono(chromator) and Spectr(ometer) sections.From these sections, the following setup can be performed:

Single or Double moving: Double moving means that the Premonochromator and the Spec-trometer scan together. Single moving means that the Premono-chromator is independent from the Spectrometer.

Security and Optimization Spectrograph SecurityThe figure page 70 shows the Security and Optimization screen.The Monochannel mode is concerned only by the spectrographsecurity. The choices are Enable (default), Inform and Disable.The Enable choice activates a laser shutter if the Spectrographscans near to the laser wavelength. The security value (enteredon the bottom of the screen) defines the security area. Thedefault Enable choice is highly recommended. The Informchoice is the same as Enable but a pop-up screen will clearlyinform the user. The Disable choice allows the user to scan up tolaser wavelength (the detector could be damaged).

Calibration screen: This feature allows the user to precisely calibrate the premono-chromator and the Spectrometer. The complete calibration pro-cedure is described in the Monochannel Mode Calibrationchapter.

Monochannel Security ValueEnter a value which defines the safety area from the laser beam.

How to setup a Multichannel CCD (Charge Coupled Device) detector?

The procedure below explains how to setup the Multichannel (CCD) Detector.

Security and OptimizationThe figure page 70 shows the Security and Optimization screen.

Spectrograph security The Spectrograph security «Enable» choice activates a lasershutter if the Spectrograph scans from the Stokes to anti-Stokeslines.If a position near to the laser wavelength is chosen, the softwarecalculates the coverage on the CCD detector. If this coveragetouches the safety area, the wavelength or wavenumber (central)will be re-calculated; the shutter will not be activated.The Inform choice will display a pop-up screen every time thesafety area is touched.

LabSpec Software 71

The Disable choice is only for those who want to manuallyexplore the near-to-laser area and in this case TAKE CARENOT TO DESTROY THE CCD DETECTOR.

First Intermediate Slit optimization (Subtractive)The First Intermediate Slit performs a filtering function on thespectrum to suppress the wavelengths interferences. Accordingto the used gratings, the slit width adapts the pass-band to coverthe current CCD Detector. Please note that a spectrum can notcompletely cover the CCD Detector if different gratings type areselected; in this case, the complete opening of the intermediateslit cannot cover the CCD Detector width. The choices are Enable (default), Inform and Disable. Using aMultichannel CCD Detector, the default Enable choice is highlyrecommended. The Inform choice is the same as Enable but apop-up screen will clearly inform the user. The Disable choiceallows the user to manually set the first Intermediate slit.

Second Intermediate Slit optimization (Additive)Using the additive Mode, the Second Intermediate Slit performsa spectrum filtering.The Enable choice will optimized the slit width according to theselected wavelength or wavenumber. The Disable choice willallows the user to manually set the slit width.

Premonochromator Optimization (Subtraction)Usually if the Premonochromator Optimization (for subtractivemode) is enable or disable the action is the same: the Premono-chromator moves the position with the Spectrometer.Exception: the Optimization is performed only in this caseFor specific choices of gratings and if the first Intermediate Slitcannot optimize to cover the total CCD area, the wavelength orwavenumber position of the Premonochromator will slightlymove to shift the spectrum to the first pixel of the CCD (if thelaser beam is located to the left of the spectrum). The spectrumwill be shifted to the right if the laser beam is located on theright.

Multichannel Security ValueEnter a value which defines the safety area from the laser beam.

Once the LabSpec software is running with the Multichannel option selected (see previouspage), select the Multichannel detector setup screen by clicking on the following icon:

LabSpec Software 72

The following CCD Detector Setup screen will be displayed:

This option allows the user to select the stripe of the used CCD for single spectrum readout, orthe window of the CCD used in image mode.

The choice of the region of the CCD used for measure allows you to limit the spectral domainof interest and to avoid reading regions without signal that would only increase the readoutand dark noises.

CCD Detector Setup

LabSpec Software 73

This following figure shows an example of a 256 x 1024 detector).

When you acquire a CCD image, the useful area will be clearly seen. So, with the parametersof the Detector setup, you can select this area. You are allowed to limit the spectral domain andthe spatial domain.

LabSpec Software 74

Monochannel Mode CalibrationThe following procedures will explain how to calibrate the System using a monochannel (PMTor IR) detector.

First Time Calibration

Usually, the T64000 System and the LabSpec software have been installed and started up byDilor-Jobin Yvon-Spex or an approved representative. Then, the «first time calibration» hasbeen already performed.

Calibration troubleshooting

The troubleshooting calibration can occur if, during a scanning, the power supply shuts downor if a link fails somewhere. The Premonochromator and Spectrometer position fields willshow . Click on the calibration button of the Spectrometer and enter the

Counter Value.

Spectrometer calibration fine tune

PreliminaryThe T64000 System can be perfectly calibrated using a known peak. This adjustment can bedone in accordance with the following limitations:

1. The calibration will be perfectly reached on the wavelength or wavenumber peak used forthe correction. For this reason, try to choose a calibration peak near to your workingregion.

2. The resulting calibration offset will be saved (if close to the counter wavelength or wave-number value). Each grating has its own value but NOT if you change the detector, forexample the Multichannel. In other words, if you change the detector or choose the Mul-tichannel, verify and eventually create a new calibration offset (the procedure isexplained below).

Procedure

1. Run the LabSpec software,

2. From the Commutations screen (see page 68) select the Monochannel Detection System,

3. Select the Monochannel working session by clicking on Monochannel icon ,

???

LabSpec Software 75

4. On the Monochannel dedicated icon bar, click on the RTD icon. The RTD will start.

Real Time Display Monochannel Parameters

Running Acquisition

LabSpec Software 76

5. Monochannel Parameters: The Monochannel parameters allows the user toprépare the acquisition parameters. Follow the instructions describe on thescreen below:

Enter here the name of the new preset

Select the acquisition channel used by the SPECTRALINK controller

Number of total acquisition cycles

add a regionEnter region parameters

Select thehardwareacquisitiongain (1,10,100)

Enter the PMT highvoltage wor-king value

of the channel 1(if the channel isvalid)

Enter thehardwareacquisitiongain of the channel 2(if valid)

Enter the safety intensity limit

LabSpec Software 77

6. Real Time Display: During the RTD acquisition, the Monochannel icon bar is replacedby the RTD Control Panel (see below)

7. Using the RTD Control Panel shown above, scan the spectrometer to the chosen «refer-ence» peak. The current cursor is represented as a vertical red line located on the screen.When the «reference peak» is founded, stop the scanning (icon located on the upper rightscreen), choose the step by step scanning mode and click on the reverse direction scan-ning up to the highest intensity level of the «reference peak» (the intensity level is dis-played on the right bottom bar of the screen). Stop the scanning at this position. Then donot change the position and follow the next step.

The figure located on the next page (page 80) shows the general LabSpec monochannelscreen during the «reference peak» search step.

8. Click on the close button of the RTD Control Panel, then click on the Close button of theMonochannel icon bar.

9. Running Acquisition: Once the Monochannel Parameters have been correctly setupand once confirmation has been performed with the Real Time Display feature, you canrun the acquisition.

Step by step scanning

Continuous scanning

Left/Right scanning toggle buttons

Current position

Integration Time

Stop RTD modeStart Time

Increment

LabSpec Software 78

10. Click on the Spectrometer Calibration button to display the calibration screen then:.

A. Select the Real Position choice

B. Enter the «reference position»

C. Click OK to validateThe spectrometer is then calibrated

LabSpec Software 79

LabSpec Softw

are80

D Control Panel

Current Intensity level

Calibration button

Stop RTD

RT

Spectrometer

Cursor line

Multichannel Mode CalibrationThe following procedures will explain how to calibrate the System using a multichannel(CCD) detector.

First Time Calibration

Usually, the T64000 System and the LabSpec software have been installed and started up byDilor-Jobin Yvon-Spex or an approved representative. Then, the «first time calibration» hasbeen already performed.

Calibration troubleshooting

The troubleshooting calibration can occur if, during a scanning, the power supply shuts downor if a link fails somewhere. The Premonochromator and Spectrometer wavelength or wave-number fields will show . Click on the calibration button of the Spectrometer

and enter the Counter Value.

Spectrometer calibration fine tune

PreliminaryThe T64000 System can be perfectly calibrated using a known peak. This adjustment can bedone in accordance with the following limitations:

1. The calibration will be perfectly reached on the wavelength peak used for the correction.For this reason, try to choose a calibration peak near to your working region.

2. The resulting calibration offset will be saved (if close to the counter wavelength or wave-number value). Each grating has its own value but NOT if you change the detector, forexample the Monochannel. In other word, if you change the detector or choose theMonochannel, verify and eventually create a new calibration offset (the procedure isexplained below).

Procedure

1. Run the LabSpec software,

2. From the Commutations screen (see page 68) select the Multichannel Detection System,

3. Click on the Multichannel calibration icon to start the calibration mode . The fol-

lowing screen will be displayed:

???

LabSpec Software 81

How to calibrate the spectrometer with a CCD detector?

The page 73 shows how to setup the CCD matrix. On the screen above, the System performs aReal Time acquisition with this additional important parameter: the central pixel of the CCDis permanently displayed. To calibrate the spectrometer, follow the procedure:

1. Choose a known reference peak and enter the reference position (see above),

2. If the spectrometer is not too de-calibrated, you should be able to see on the screen thisreference peak.

3. Try, by entering a new position value, to center the reference peak on the central pixel.This could be performed by successive estimations. Zooming the central area willincrease the calibration setting.

4. Once the reference peak is perfectly set on the central pixel, click on the Calibration but-ton of the Spectrometer panel. Then follow the procedure described below:

Central pixel

Reference peak

Spectrometer position

LabSpec Software 82

Premonochromator calibration and optimization (Subtractive mode)

The T64000 System is composed of three stages: the first two stages are called Premonochro-mator, the last stage is the Spectrometer.

By default, the Premonochromator stages are configured as a subtractive optical design. In thiscase, the Premonochromator performs a filtering function. If the highest results are required, aPremonochromator calibration is necessary to perform a band-pass on a working area. Theprocedure described below will show how to optimize a calibration.

The goal of this procedure is to close as much as possible the intermediate slit while the refer-ence peak stays «visible» on the center of the CCD detector.

1. Calibrate the Spectrometer using the procedure described on page 81; at the end of thisprocedure, the reference peak is displayed at the center of the CCD detector,

2. From the Security and Optimization screen (see page 70), choose Disable the Premono-chromator and First Slit optimization,

3. From the Premonochromator Control Panel click on the Single Moving choice,

4. On the same Control Panel, click on the following button to display the slits listand select the First Intermediate Slit.

5. Click on the CCD Calibration icon to run the calibration Real Time acquisition,

6. In the slit width field, enter a smaller value than displayed (about 10% less) and verifythat the peak is always visible and centered on the CCD. Continue to reduce the interme-

A. Select the Real Position choice

B. Enter the «reference wavelength»

C. Click OK to validateThe spectrometer is then calibrated

o

LabSpec Software 83

diate slit. If the spectrum disappears, that mean that the position (Premonochromator) isnot centered on the beam path. To re-center the filter, enter a new position value in thePremonochromator field, more or less you have to try, or slightly open the intermediateslit to re-display the spectrum. This is a step by step procedure, and once reached theminimum slit width value, you know that the premonochromator is optimally adjustedand the Acquisition session can be now performed.

Please refer to the Training Course Manual for detailed explanations.

1 2 3 4 5 6

7 8 9 10 11

1. Available slits selection list

2. Current slit calibration

3. Single moving (Premonochromator and spectrome-ter are independent) or double moving mode switch.

4. Premonochromator calibration

5. Security and Optimization modes

6. Spectrometer calibration

7. Close the current slit

8. Slit width field

9. Open the current slit

10. Premonochromator position field

11. Spectrometer wavelength field

Control Panel features

o

LabSpec Software 84

Interac-tive Index
Symbols
*Const 20+Const 20

Numerics3D MODE 50

AAcquisition panel 12Acquisition parameters 41Add a spectrum 25additive 69All spectra window 38Analysis mode 68Apodisation 29APPROX 32Attachment 21Auto Repeat 53Auto save option 52Autofocus 42

BBaseline correction 19Baseline correction example 62binning 39, 57, 61

CCalibration (spectrometer,

monochannel) 79Calibration (XY stage) 43CCD detector calibration

(T64000) 82CCD image adjustment 37CCD Matrix setup (T64000) 73Color Tiff 16Commutations screen 75Compressed Tiff 15, 16Configuration (system) 45confocal hole 56Confocal mapping 58Confocal value 42Constant for addition 18Constant for multiplication 18Correction 21cursor for working 13Cursor normalization 16cursors (Red, Green, Blue) 35

DData size 38

LabSpec Software

Decomposition 35

Deconvolution 35Definition of the function 18Delete the object in the window 14Depth 40Description of the different icons 13detection of the peak 30detector 39Detector parameters 42Detector setup (T64000) 74Deviation 21Deviation factor 33DFT 29Dilor format 15Double moving 71

EEdit, Copy data 48Edit, Copy picture 48Edit, Copy Text 48Edit, Paste Data 48Edit, Paste picture 48Edit, Paste Text 48Exchange a spectrum 24Exit LabSpec 47Extended Tiff 15, 16Extract procedure 35Extract profile 25Extracted spectrum 34

FFALSE 33File, Delete 47File, open 47File, Print 47File, Save 47File, Split 47Filter 12Filtering 26Fit peak width 14Fitting (peak) 63Focusing the laser 57Format, BEHAVIOUR 48Format, COLORS 49Format, IMPOSITION 49Format, SUPERPOSITION 49Format, VIEW 48Formats (Image) 16formats for spectra saving 15Fourier transform (filtering) 29

Index - 85

Index
GGaussian 32Gaussian/Lorentzian 32grating (choice) 57GRAY 33Green/blue ratio 35
HHole 12hole aperture 12Horizontal shift 13How to acquire a spectrum 56How to record spectra at selected

points? 57

IIFT 29Information about object 16Initialization 69Ins/Del 20Insert a spectrum 23Integral calculation 33Intensity adjustment 13inverse Fourier transform 29

KKinetic 60

LLabel of the peak 14LabRam 57, 58, 59LabRam Infinity 57, 58Laser 12laser 56Laser Shutter 69Line scan illumination 59Load 14Lorentzian 32

MManual addition 13Mathematical filtering 29Mathematical processing 17Menu Bar Description 47Model 36model 65Models 65Monochannel Mode

Calibration 75Monochannel/Multichannel

LabSpec Software

Setup 70

Morphological images 43Move peak maxima 14MUL, SUB, ADD, DIV 22Multi window 17Multichannel Mode Calibration 81multiply 14

NNormalization 16, 22number of accumulations 12

Oobject 51offset (autofocus) 42Operations between two spectra 18Operations for the baseline 20Option menu commands 51

PPalette window 33peak detection 30Peak elimination 13Peak fitting 30, 63PeakElm 27Polynomial filtering 28Position of the spectrograph 12Premonochromator calibration

(T64000) 83Premonochromator calibration and

optimization 83Print page 16printer page configuration 47Printer set-up 47Profile 23

QQuit LabSpec 47

RRaman/Fluo 35Recording time 12Refresh time 42Removing spectrum 23Reorganize 53RTD acquisition (T64000) 78

Ssave a TV image 15save spectra 15Save the active object 14

Index - 86

scale of a spectrum 13

Index
scanner calibration 44Scanning area 41Scanning device 59Scheme 46Second/Third Stage 69Security and Optimization
(T64000) 71setup Multichannel 71Shape correction 13Shift 13Single moving 71slit apertures 57Spectra Calc format 15Spectral 39Spectral images recording 37Spectral mapping 35Spectral mapping window 38spectrograph setup 56Spectrometer calibration

(monochannel) 75Spectrum adjustment 36spectrum adjustment 56Spectrum correction 22Spectrum recording 37Spike removing 41Standard Tiff 16Stop 46subtractive 69Summa 35system configuration 45

TT64000 Configuration Setup 68T64000 Macro-sample 68T64000 Microscope 68T64000 Premonochromator 69T64000 spectrometer 69T64000 System 67Text format 15, 16thr(%) 21Threshold 21TIME 32Time 12, 39, 40Time kinetic recording 60TRUE 34TSF format 53types of cursors 46

Uunits 51

LabSpec Software

VVertical shift 13Video image 36video image 57

XX scanning 41

ZZ scanning 61ZERO 22Zoom 13, 20, 34

Index - 87

labspec© software

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