micro lab final presentation
TRANSCRIPT
THE ISOLATION, CHARACTERIZATION, AND IDENTIFICATION OF A BACTERIUM FROM:STAPHYLOCOCCUS EPIDERMIDIS
Taken from: Stillwell bathroom paper towel dispenser
Hailey Medder12/08/2016
CULTURE CHARACTERISTICSUnit Being Observed
Size Shape Color
Colony 1.0 millimeter Cocci (clusters)
Opaque cream
Cell 0.5-.0.7 micrometers
Spherical ‘points’
Opaque cream
Photo by: Brittany H. from StudyBlue “Microbiology Final”
Culture Growth CharacteristicsTemperature (oC)
Salt (%NaCl)
pH
Oxygen
-Does not need oxygen due to being a facultative anaerobe
45
15
4018-40
Salt% 1 2 3 4 5 6 pH0% + + + - - - 35% + + + + + + 510% + + + + + + 715% - - - + + + 9
Salt and pH Treatment
0.0 10
2.0 4.0
6.0
8.00.0-0.5
57.0-7.45
7.0
9.0
Enteropluri
Glucose fermentation: YesGas production:
PartialLysine decarboxylation: NoOrnithine decarboxylation: NoSulfur reduction to H2S:No
Indole produced: NoAdonitol fermentation: NoLactose fermentation: NoArabinose fermentation: PartialSorbitol fermentation: NoAcetoin production (VP):NoDulcitol fermentation: No
Phenylalanine deamination: NoUrea hydrolysis: YesCitrate utilization: No
Motility- No
Oxidase- No
Catalase- Yes
Source: ResearchGate
Source: Microbeinfo.com
Blood agar
Maconkey agar
Although there were tons of colonies present, the S. epidermidis did not hemolyze the blood agar.
There was absolutely no growth on this Maconkey agar.
Positive gram stain
DNA SEQUENCES: DATA FOR 16S RDNA ANALYZED USING THE RIBOSOMAL DATABASE PROJECT
Database Name % Match EnvironmentType Cultures Only
Staphylococcus epidermidis
99.3 Clinical isolates in a Japanese zoo
Staphylococcus capitis 97.3 Japanese veterinary school Staphylococcus capitis 96.4 Skin samplesStaphylococcus caprae 96.3 Japanese rRNA samplesStaphylococcus saccharolyticus
95.8 Tissue samples
All RDP RecordsStaphylococcus epidermidis
100 GenBank in Northern Ireland
Staphylococcus epidermidis
100 Topoisomerase of isolates
Staphylococcus sp. 100 Wastewater in IndiaStaphylococcus sp. 100 Bluegill mucus in JapanStaphylococcus epidermidis
100 GenBank in Madrid Spain
DNA Sequences: Data for 16s rDNA Analyzed using the Ribosomal Database Project
Phylum: FirmicutesClass: BacilliOrder: BacillalesFamily: StaphylococcaceaGenus: Staphylococcus
COMPARATIVE CHARACTERISTICS TABLE
Test S. aureus S. caprae S. haemolyticus
S. epidermidis
Growth at 40ºC + + + +Growth at pH 4 - - - -Growth in 2% NaCl + + + +Hydrolysis of Tween 80
- - - -
Hemolyze blood cells
+ + - -
Acid from glucose + - + +Acid from raffinose - - - -Triclosan resistnce - - - -Capsules present + + + +Nitrate reduction - + + -
HAVE YOU IDENTIFIED A NEW SPECIES OF BACTERIA?
No
No I have not identified a new bacterial species. I know this because the data found on this species was published in multiple scholarly articles as well as in the Bergey’s Manual dating back to the early 1880’s.
ABSTRACT
Cells are Gram-positive, non-spore-forming, non-motile spheres approximately 0.5 μm in diameter. Colonies are creamy, circular, smooth, and 1.0 μm in diameter after 48 hour incubation at 37°C on R2A. The species could grow over10 μm from a range of salt concentrations from 0-0.5%, from pH 7.0-7.45, and at 37°C. Cultures are facultatively anaerobic, catalase-positive, and oxidase-negative. Colonies are non-hemolytic. Acid is produced from d-glucose, maltose, and partially from l-arabinose, but not from glycerol, glycogen, inulin, d-lactose, d-mannitol, raffinose, d-ribose, d-sorbitol, sucrose, trehalose or d-xylose. Hippurate, gelatin, and aesculin are not hydrolysed but starch and urea are. It does not utilize citrate. Nitrate is not reduced. Acetoin production is negative. Cells produce capsules but not flagella. No H2S is formed metabolically. DNAse and lipase activity are not present. Cells are susceptible to penicillin. The 16S rDNA sequence obtained from the culture revealed it to belong to the phylum Firmicutes, class Bacilli, order Bacillales, family Staphylococcaceae, and genus Staphylococcus. The closest relative of the culture is S. capitis, which has a 97.3% sequence homology to the isolate.
The strain was isolated from the paper towel dispenser handle in Cullowhee, North Carolina and is given the name Staphylococcus epidermidis.
REFERENCESATCC, https://www.atcc.org/Products/All/CRM-12228.aspx#culturemethod
S., F. A., Buchanan, R. E., Gibbons, N. E., & B. (1975). Bergey's Manual of Determinative Bacteriology. Taxon, 24(2/3), 377. doi:10.2307/1218353
Staphylococcus Genus Characteristics, http://biolabs.tmcc.edu/Micro%20Web/Staph.pdf
IJSEM database
M. B. (2004, September 27). Staphylococcus epidrmidis. Staphylococcus Epidrmidis. Retrieved December 6, 2016, from http://web.uconn.edu/mcbstaff/graf/Student presentations/S epidermidis/sepidermidis.html
https://www.ncbi.nlm.nih.gov/pubmed/9554167
Questions? Please be kind!