micro lab notes
DESCRIPTION
ParasitologyTRANSCRIPT
BACTERIOLOGY 231
PLATE M
EDIA
ME
DIA
C
AR
BO
HY
DR
AT
E
INH
IBIT
OR
p
H I
ND
ICA
TO
R
RE
SU
LT
Alp
ha
he
mo
lysi
s
Inco
mp
lete
or
pa
rtia
l ly
sis
wit
h
gre
en
ing
Be
ta h
em
oly
sis
Co
mp
lete
ly
sis
wit
h c
olo
rle
ss
zon
e [
dif
fusi
on
of
he
mo
glo
bin
into
th
e s
urr
ou
nd
ing
me
diu
m]
BA
P
Blo
od
ag
ar
Ou
tda
ted
hu
ma
n b
loo
d o
r 5
%
de
frib
ina
ted
sh
ee
p b
loo
d
(pre
ferr
ed
)
Try
pti
case
So
y A
ga
r o
r
Nu
trie
nt
ag
ar
{No
nse
lect
ive
Dif
fere
nti
al
ag
ar}
Ga
mm
a h
em
oly
sis
No
nh
em
oly
tic
Lyse
d r
ed
blo
od
ce
lls
Try
pti
case
So
y A
ga
r o
r
Nu
trie
nt
ag
ar
H
em
oly
tic
CA
P
Ch
oco
late
ag
ar
X f
act
or
(he
min
)
V f
act
or
or
NA
D (
nic
oti
na
mid
e
ad
en
ine
din
ucl
eti
de
,
coe
nzy
me
)
{No
nse
lect
ive
Dif
fere
nti
al
ag
ar}
No
nh
em
oly
tic
PE
A
Ph
en
yle
thyl
{Se
lect
ive
& D
iffe
ren
tia
l a
ga
r}
Gra
m (
-) i
nh
ibit
or
{Se
lect
ive
ag
ar}
✔
S
tap
hy
loco
ccu
s sp
.
alc
oh
ol
ag
ar
Inh
ibit
s sw
arm
ing
of
Pro
teu
s
{Se
lect
ive
& D
iffe
ren
tia
l a
ga
r}
Inh
ibit
s G
ram
(+
)
✔ Y
ell
ow
co
lon
ies
[pH
6.8
]
Co
ag
ula
se (
+)
Sta
ph
. sp
.
So
me
Mic
roco
ccu
s &
En
tero
cocc
us
MS
A
Ma
nn
ito
l sa
lt
ag
ar
Be
ef
ext
ract
Ma
nn
ito
l 7
.5%
Na
Cl
Ph
en
ol
red
✔
Sma
ll r
ed
co
lon
ies
wit
h r
ed
/pu
rple
zo
ne
[pH
8.4
]
Mo
st G
ram
(-)
Co
ag
ula
se (
-) S
tap
h.
Sp
.
✔G
ree
n m
eta
llic
sh
ee
n
(LF
)
E.
coli
(la
cto
se f
erm
en
ter)
✔G
ree
n m
eta
llic
sh
ee
n
w/
fish
eye
En
tero
ba
cte
r a
ero
ge
ne
s (R
LF)
✔ L
arg
e p
urp
le m
uco
id
(LF
)
Kle
bsi
ell
a p
ne
um
on
iae
EM
B
Eo
sin
me
thyl
en
e
blu
e a
ga
r
{S
ele
ctiv
e &
Dif
fere
nti
al
ag
ar}
Inh
ibit
s G
ram
(+
)
Lact
ose
E
osi
n
Me
thyl
en
e b
lue
✔Y
ell
ow
to
co
lorl
ess
(pin
k)
colo
nie
s (N
LF)
Sa
lmo
ne
lla
typ
hi
Sh
ige
lla
so
nn
ei
Pro
teu
s vu
lga
ris
Pse
do
mo
na
s a
ero
gin
osa
✔P
ink
to r
ed
co
lon
ies
[pH
<6
.8]
Lact
ose
fe
rme
nti
ng
Gra
m (
-)
MA
C
Ma
cCo
nk
ey
ag
ar
{Dif
fere
nti
al
ag
ar}
Lact
ose
C
ryst
al
vio
let
Bil
e s
alt
s N
eu
tra
l re
d
✔Y
ell
ow
to
co
lorl
ess
colo
nie
s [p
H 6
.8-8
.0]
No
n-l
act
ose
fe
rme
nti
ng
Gra
m (
-)
✔B
lue
to
gre
en
w/
bla
ck c
en
ter
Pro
vid
en
cia
re
tte
gri
Mo
rga
ne
lla
mo
rga
nii
Sh
ige
lla
dy
sen
teri
ae
✔B
lue
to
gre
en
w/o
bla
ck c
en
ter
Sa
lmo
ne
lla
typ
hi
Pro
teu
s vu
lga
ris
Pro
teu
s m
ira
bil
is
✔Y
ell
ow
to
ora
ng
e w
/
bla
ck c
en
ter
Cit
rob
act
er
fre
un
dii
Sa
lmo
ne
lla
ari
zon
ae
HE
A
He
kto
en
en
teri
c a
ga
r
{Se
lect
ive
& D
iffe
ren
tia
l a
ga
r}
Inh
ibit
s n
orm
al f
lora
co
lifo
rm
of
the
lo
we
r G
I tr
act
[Sa
lmo
ne
lla
an
d S
hig
ell
a
spe
cie
s fr
om
oth
er
Gra
m (
-)
en
teri
c o
rga
nis
ms]
Lact
ose
Su
cro
se
Sa
lici
n
Bil
e s
alt
s
Cit
rate
Bro
mth
ymo
l
blu
e
✔Y
ell
ow
to
ora
ng
e w
/o
bla
ck c
en
ter
E.
coli
Kle
bsi
ell
a p
ne
um
on
ia
En
tero
ba
cte
r a
ero
ge
ne
s
Sh
ige
lla
so
nn
ei
Se
rra
tia
ma
rce
sen
s
✔ Y
ell
ow
co
lon
ies
w/
bla
ck c
en
ter
Cit
rob
act
er
fre
un
dii
Pro
teu
s vu
lga
ris
Pro
teu
s m
ira
bil
is
✔Y
ell
ow
co
lon
ies
w/o
bla
ck c
en
ter
E.
coli
Kle
bsi
ell
a p
ne
um
on
ia
En
tero
ba
cte
r a
ero
ge
ne
s
Pro
vid
en
cia
re
tte
ri
Se
rra
tia
ma
rce
sen
s
✔R
ed
co
lon
ies
w/
bla
ck c
en
ter
Sa
lmo
ne
lla
typ
hi
XLD
Xyl
ose
-lys
ine
de
oxy
cho
late
{Se
lect
ive
& D
iffe
ren
tia
l a
ga
r}
Inh
ibit
s n
orm
al
flo
ra c
oli
form
s
[Pa
rtia
lly
E.
coli
& s
wa
rmin
g o
f
Pro
teu
s]
Xyl
ose
D
eo
xych
ola
te
Ph
en
ol
red
[H2S
: fe
rric
am
mo
niu
m
chit
rate
;
sod
ium
thio
sulf
ate
]
✔R
ed
co
lon
ies
w/o
bla
ck c
en
ter
Sh
ige
lla
dy
sen
teri
ae
✔P
ink
to r
ed
co
lon
ies
w/o
bla
ck c
en
ter
No
rma
l fl
ora
co
lifo
rms
✔Y
ell
ow
to
co
lorl
ess
w/o
bla
ck c
en
ter
Sh
ige
lla
dy
sen
teri
ae
Sh
ige
lla
fle
xne
ri
Sh
ige
lla
bo
yd
ii
SS
A
Sa
lmo
ne
lla
-
Sh
ige
lla
ag
ar
{Se
lect
ive
& D
iffe
ren
tia
l a
ga
r}
Inh
ibit
ion
of
no
rma
l fl
ora
coli
form
s
Inh
ibit
s G
ram
(+
) &
(-)
Lact
ose
Bil
e s
alt
s
Bri
llia
nt
gre
en
Ne
utr
al
red
[H2S
: fe
rric
am
mo
niu
m
chit
rate
;
sod
ium
thio
sulf
ate
]
✔Y
ell
ow
to
co
lorl
ess
w/
bla
ck c
en
ter
Sa
lmo
ne
lla
typ
hi
✔Y
ell
ow
co
lon
ies
V.
cho
lera
V.
alg
ino
lyti
cus
TC
BS
Th
iosu
lfa
te
Cit
rate
Bil
e
salt
Su
cro
se
ag
ar
{Se
lect
ive
& D
iffe
ren
tia
l a
ga
r}
Iso
late
Vib
rio
sp
.
Su
cro
se
So
diu
m
citr
ate
So
diu
m
thio
sulf
ate
Oxg
all
Bro
mth
ymo
l
blu
e
[H2S
: fe
rric
am
mo
niu
m
chit
rate
;
sod
ium
thio
sulf
ate
]
✔B
lue
-gre
en
co
lon
ies
V.
pa
rah
ae
mo
lyti
cus
V.
vu
lnif
icu
s
Ae
rom
on
as
Psu
ed
om
on
as
✔ B
lack
co
lon
ies
wit
h
me
tall
ic c
he
en
Sa
lmo
ne
lla
typ
hi
✔Li
gh
t g
ree
n c
olo
nie
s S
. g
all
ina
rum
S.
cho
lera
esu
is
S.
pa
raty
ph
i
BS
A
Bis
mu
th
sulf
ate
ag
ar
{Se
lect
ive
& D
iffe
ren
tia
l a
ga
r}
Iso
late
Sa
lmo
ne
lla
sp
.
Inh
ibit
Gra
m (
+),
la
cto
se
ferm
en
ter
& s
hig
ell
ase
Bis
mu
th
sulf
ate
Bri
llia
nt
gre
en
[H2S
: fe
rric
sulf
ide
]
solu
ble
✔N
o g
row
th
Oth
er
en
teri
cs
BACTERIOLOGY 231
TUBE M
EDIA
ME
DIA
F
OR
MA
TIO
N
CA
RB
OH
YD
RA
TE
p
H I
ND
ICA
TO
R
H2
S IN
DIC
AT
OR
R
ESU
LT
TS
I
Tri
ple
Su
ga
r
Iro
n a
ga
r
Bu
tt S
lan
t G
luco
se
Lact
ose
Su
cro
se
Ph
en
ol
red
F
err
ou
s su
lfa
te
A/A
G (
4)
E.
coli
En
tero
ba
cte
r a
ero
ge
ne
s
Pa
nto
ea
ag
glo
me
ran
s
Kle
bsi
ell
a p
ne
um
on
ia
A/A
G+
or
K/A
G+
(5
) C
ito
ba
cte
r fr
eu
nd
ii
Sa
lmo
ne
lla
ari
zon
ae
A/A
G o
r K
/AG
(4
a)
Cit
rob
act
er
ko
seri
Se
rra
tia
ma
rce
sce
ns
Ha
fnia
alv
ei
K/A
G+
P
rote
us
vulg
ari
s
Pro
teu
s m
ira
bil
is
Sa
lmo
ne
lla
pa
raty
ph
i
Sa
lmo
ne
lla
ch
ole
rae
suis
Ed
wa
rdsi
ell
a t
ard
a
A/A
or
K/A
Y
ers
inia
en
teri
coli
tica
Sh
ige
lla
so
nn
ei
K/A
(2
) S
hig
ell
a d
yse
nte
ria
e
Sh
ige
lla
fle
xne
ri
Sh
ige
lla
bo
yd
ii
K/A
+ (
3)
Sa
lmo
ne
lla
typ
hi
K/K
(1
) P
seu
do
nm
on
as
ae
rog
ino
sa
Alc
ali
ge
ne
s
ME
DIA
F
OR
MA
TIO
N
AM
INO
AC
ID
CA
RB
OH
YD
RA
TE
p
H
IND
ICA
TO
R
H2
S
IND
ICA
TO
R
RE
SULT
LIA
Lysi
ne
Iro
n
Ag
ar
[Ly
sin
e
de
am
ina
tio
n]
Bu
tt S
lan
t Ly
sin
e
Glu
cose
B
rom
cre
sol
pu
rple
Fe
rric
am
mo
niu
m
citr
ate
K/K
E
. co
li
En
tero
ba
cte
r a
ero
ge
ne
s
Kle
bsi
ell
a p
ne
um
on
ia
Se
rra
tia
ma
rce
sce
ns
Ha
fnia
alv
ei
K/A
P
an
toe
a a
gg
lom
era
ns
Cit
ob
act
er
fre
un
dii
Sh
ige
lla
dy
sen
teri
ae
Sh
ige
lla
fle
xne
ri
Sh
ige
lla
bo
yd
ii
R/A
P
rote
us
Pro
vid
en
cia
Mo
rga
ne
lla
K/A
G+
S
alm
on
ell
a t
yph
imu
riu
m
Sa
lmo
ne
lla
ari
zon
ae
Sa
lmo
ne
lla
typ
hi
Sa
lmo
ne
lla
pa
ray
ph
i
Sa
lmo
ne
lla
ch
ole
rae
suis
Ed
wa
rdsi
ell
a t
ard
a
ME
DIA
P
RIN
CIP
LE
FO
RM
AT
ION
M
ED
IUM
R
EA
GE
NT
PO
SIT
IVE
N
EG
AT
IVE
Ind
ol
Pro
du
ctio
n
Te
st
Th
e o
rga
nis
m
oxi
diz
ed
try
pto
ph
an
to i
nd
ol
by
tryt
op
ha
na
se.
Bu
tt
Try
pto
ne
bro
th
Eh
rlic
h’s
or
Ko
va
c’s
(p-
am
ino
be
nza
lde
hy
de
)
Re
d R
ing
Y
ell
ow
/Bro
wn
Rin
g
Me
thyl
Re
d T
est
Th
e o
rga
nis
m
ferm
en
ts g
luco
se t
o
py
ruvi
c a
cid
resu
ltin
g in
mix
ed
aci
d p
rod
uct
s
lea
din
g t
o d
ecr
ea
sed
pH
(4
.5-5
.5)
of
the
me
diu
m a
nd
ta
ke
s
the
co
lor
of
the
ind
ica
tor
Bu
tt
MR
VP
(Me
thyl
Re
d V
og
es
Pro
ska
ue
r)
me
diu
m
Me
thyl
re
d i
nd
ica
tor
Re
d
solu
tio
n
Ye
llo
w
solu
tio
n
Vo
ge
s
Pro
ska
ue
r
Te
st
Th
e o
rga
nis
m
ferm
en
ts g
luco
se
pro
du
cin
g
ace
tylm
eth
ylca
rbin
ol
(ace
toin
) a
nd
con
ve
rte
d t
o
dia
cety
l in
th
e
pre
sen
ce o
f o
xyg
en
an
d K
OH
.
Bu
tt
MR
VP
V
P R
ea
ge
nt
A:
Alp
ha
na
pth
ol i
n
ab
solu
te m
eth
yl
alc
oh
ol
VP
Re
ag
en
t B
:
KO
H w
ith
cre
ati
ne
Pin
k t
o R
ed
colo
r
Ye
llo
w o
r
cop
pe
r-li
ke
Cit
rate
Uti
liza
tio
n
Te
st
Th
e o
rga
nis
m
uti
lize
s th
e c
itra
te
pro
du
cin
g a
mm
on
ia
an
d c
on
ve
rte
d t
o
am
mo
niu
m
hy
dro
xid
e.
Th
is
alk
ali
ne
co
mp
ou
nd
rais
es
the
pH
of
the
me
diu
m a
nd
ta
ke
s
the
blu
e c
olo
r.
Sla
nt
SC
A
(Sim
mo
n
Cit
rate
Ag
ar)
Bro
mth
ymo
l b
lue
(in
dic
ato
r)
Pru
ssia
n
blu
e c
olo
r
Gre
en
co
lor
Ure
ase
Te
st
Ure
ase
sp
lits
th
e
ure
a m
ole
cule
s in
to
am
mo
nia
, ca
rbo
n
dio
xid
e a
nd
wa
ter.
Am
mo
nia
re
act
s in
solu
tio
n t
o f
orm
alk
ali
ne
co
mp
ou
nd
,
am
mo
niu
m
carb
on
ate
, w
hic
h
resu
lts
in a
n
incr
ea
sed
pH
of
the
me
diu
m a
nd
a c
olo
r
cha
ng
e i
n t
he
ind
ica
tor
to p
ink-
red
.
Bu
tt (
Bro
th)
Ure
a
bro
th
Ph
en
ol
red
(in
dic
ato
r)
Pin
k-re
d
No
co
lor
cha
ng
e
Mo
tile
(gro
wth
pa
tte
rn –
aw
ay
fro
m
ino
cula
tio
n
lin
e)
No
nm
oti
le
(gro
wth
pa
tte
rn i
s
con
fin
ed
)
H2S
pro
du
ctio
n (
Bla
cke
nin
g
of
the
bu
tt)
Su
lfid
e
ind
ole
mo
tili
ty
(SIM
)
B
utt
(S
em
i-
soli
d)
Ind
ol
pro
du
ctio
n (
red
rin
g)