NaviCell Web ServiceData visualization tutorial

NaviCell Web Service: description

Map: https://navicell.curie.fr/navicell/maps/cellcycle/master/index.html

Data: https://navicell.curie.fr/doc/ws/data_viz_tuto_data_files.zipCopy number variations

mRNA expressionMutations

•NaviCell Web Service is a powerful data visualization tool that allow users to load, manage and visualize a large number of experimental data types in the context of molecular interaction maps.

•The user can upload expression data for mRNA, microRNA, proteins, mutation, copy-number data, and simple gene lists. The input data format is a standard tab-delimited text file.

•Users can upload sample annotation files that can be used to automatically calculate average values for defined groups of samples.

•Different options are available to display data values: heatmaps, barplots, glyphs and map staining.

Data visualization example

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Load dataIn the Data visualization menu click ‘Load Data’:Chose File: table in a tab-delimited text file format (e.g. tcga_expression.txt)Name: type name of dataset ( e.g. expression)Type: indicate type of data (e.g. mRNA expression data)Import (several datasets can be loaded one after another)Done

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Load annotation tableIn the Data visualization menu click ‘Sample Annotations’:Step 1 Chose File: table in a tab-delimited text file format (e.g. ovca_sampleinfo.txt) orSample annotation URL: to upload the annotation fileImport annotationsStep 2 Sample Annotations: Check boxes to create groups (e.g. IntrinsicExprClassJCI)ApplyDone

Step 1Step 2

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Check your data

In the Data visualization menu click ‘My Data’:Check available types of data, samples, gene list, group created according to the annotation table, etc.

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visualize copy number data using heatmap In the Data visualization menu click ‘Drawing Configuration’:Step1 Chart Type: Heatmap/ Apply/ click ‘configuration’Step 2 Heatmap Configuration Editor: Select a sample or group (e.g. TCGA_041332, TCGA_24_1560)/ Select a data table (e.g. ‘copynumber’)/ Size (e.g. Medium)/ click ‘config’Step 3 copynumber Datatable Color Configuration: Samples/assign color to value/ Apply

Step 1Step 2

Step 3

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Copy number variations-heatmapSamples:TCGA_24_1560 tetraploid, prolifirativeTCGA_04_1332 tetraploid, mesenchimal

Zoom in on Cyclin E*:Clicking on the heatmap opens the heatmap configuration editor where values can be viewed

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visualize copy number data using barplot In the Data visualization menu click ‘Drawing Configuration’:Step1Chart Type: Barplot/ Apply/ click ‘configuration’Step 2 Barplot Configuration Editor: Select a sample or group (e.g. TCGA_041332, TCGA_24_1560)/ Select a data table (e.g. ‘copynumber’)/ Size (e.g. Medium)/ click ‘config’Step 3 copynumber Datatable Color Configuration: Samples/assign color to value/ Apply

Step 1Step 2

Step 3

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Copy number variations-bar plotSamples:TCGA_24_1560 tetraploid, prolifirativeTCGA_04_1332 tetraploid, mesenchimal

Zoom in on Cyclin E*:Clicking on the heat map opens the heat map configuration editor where values can be viewed

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visualize mutations data using glyph In the Data visualization menu click ‘Drawing Configuration’:Step1 Glyphs: Glyph 1/ Apply/ click ‘configuration’Step 2 Glypg 1 Configuration Editor: Sample or group (e.g. TCGA_25_1624)/ in ‘Shape, ‘Color’ and ‘Size’, chose ‘mutations’, click ‘config’ on each one of the configurationsStep 3 mutations Datatable Shape Configuration: Samples/assign shape of glyphs to value/ ApplyStep 4 mutations Datatable Color Configuration: : Samples/assign color to value/ ApplyStep 5 mutations Size Shape Configuration: : Samples/chose value for glyphs size/ Apply

Step 1Step 2

Step 3Step 4

Step 5

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Mutations-glyphZoom in on mutated genes:p53, p130*, RBM25

Distribution of mutated gene on the map

Sample:TCGA_25_1634 tetraploid, mildly proliferative

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In the Data visualization menu click ‘Drawing Configuration’:Step1 Map staining: Apply/ click on ‘configuration’Step 2 Map staining Configuration Editor: Sample or group (e.g. TCGA_24_1560)/ in ‘Color’ chose ‘expression’/ click ‘config’Step 3 expression Datatable Color Configuration: Samples/assign color to value/ Apply

visualize expression data using map staining

Step 1Step 2 Step 3

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Expression-map staining

TCGA_04_1332 tetraploid, mesenchymal TCGA_24_1560 tetraploid, proliferative

The patters of mRNA expression visualized as map staining are different in mesenchymal and proliferative types of ovarian cancer samples.

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Several type of data can be visualized at the same map using a combination of visualization modes described previous in the tutorial.

In the Data visualization menu click ‘Drawing Configuration’:Step1 Click Heatmap, Glyphs, Map Staining/ click ‘config’ on each one of the configurationsStep2 Heatmap Configuration Editor: follow instructions indicated on the page 5Step 3 Glypg 1 Configuration Editor: follow instructions indicated on the page 9Step 4 Map staining Configuration Editor: follow instructions indicated on the page 11

visualize several type of data simultaneously

Step 1Step 2

Step 3Step 4

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Mutations-glyphCopy number-heat mapExpression-map staining

TCGA_25_1634 tetraploid, proliferative

Zoom in on p130* in several complexes

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In the Data visualization menu click ‘Drawing Configuration’:Step1 Map staining: Apply/ click on ‘configuration’Step 2 Map staining Configuration Editor: Sample or group (e.g. Differentiated)/ in ‘Color’ chose ‘expression’/click ‘config’Step 3 expression Datatable Color Configuration: Groups/assign color to value/ Apply

visualize expression data for groups of samples using map staining

Step 1 Step 2 Step 3

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Expression-map stainingon group of samples

Differentiated Mesenchymal

Immunoreactive Proliferative

Groups: Intrinsic expression classes

The patters of mRNA expression visualized as map staining are varying in four intrinsic classes of ovarian cancer samples, indicating different regulation of processes depicted on the map.

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