newcomerto Pharmacia Gel Filtration Media

new agarose gels inbead formoooooooooooo

Sepharose(Prepared according to S. Hjerte'n, Uppsala) for gel filtration ofviruses and very high molecular weight macromolecules suchas proteins, polysaccharides and nucleic acids.

Sepharose 4B Approx. fractionation range (Mw) 300,000—3 millionSepharose 2B Approx. fractionation range (Mw) 2 million—25 million

Separation of T-lib«lled adinovlnii andpoliovirut on Siphvou 2B.

Purification of a cru'ds preparation of tatall-ili tobacco naouii virui on Sapharote 4B.

FURTHER INFORMATION ONSEPHAROSE® FROM:

PHARMACIA(GREAT BRITAIN) LTD

LEADERS IN GEL FILTRATIONSINCLAIR HOUSE • THE AVENUE • WEST EAUNG • LONDON W.13or from Phinuclt Flu ttiBlcali • Upptala • Swnitn

Jnl. of Cell Sci., Vol. 2, No. 2 to

Quick Estimate of D.N.A.?The Barr & Stroud integrating microdensitometer measures the relative amount

of D.N.A. or R.N.A. in a sample of stained cells. It can also be used on other

substances which can be dyed selectively.

Setting up the instrument, once a slide has been prepared, takes only a few

minutes and readings of integrating density can be made in seconds.

Please write or telephone for pamphlet No. 1239/CS

BARR & STROUD LIMITED ENGINEERS & INSTRUMENT MAKERSAnnieslandGlasgow, W. 3Tel: Jordanhlll 9601Telex: 778114

1 Pall Mall EastLondon, S.W.1Tel: 01-930 1541Telex: 261877

VICKERS LIMITED • VICKERS INSTRUMENTSHAXBY ROAD - YORK

PURLEY WAY • CROYOON • CR9 4HN

The Patholux microscope isan economically designed in-strument with all controls forf o c u s i n g and I l l u m i n a t i o ngrouped on a central panel withthe stage and condenser adjust-ments directly above. All opera-tions can be carried out witheither the left or the right hand.All focusing and stage motionsare on ball bearings.

The light source is a built-in100 watt tungsten halogen lampwith full controls for Kohler illum-ination. The variable intensitycontrol Is at the front of thecontrol panel and adequate lightis available for micro-projection.

The large stage (7j" x 9' withgraduated 2'x3" movement) doesnot move in focusing, therebygiving It the stability necessaryin micro-manipulation and otherspecial techniques. The body ofthe microscope can be raised onan extra slide to give a clearancewhich will accommodate verylarge objects such as tissueculture flasks and other experi-mental set-ups.

Two beam splitter boxes areof fered, onevarying Instan-taneouslyfrom50% visual—50% camera to100% cameraand the otherf r o m 1 0 0 %

visual to 100% camera. Monocu-lar or b i nocu la r heads areattached to the prism box asrequired.

Any of the Vickers camerascan be quickly mounted andInterchanged. The cameras avail-able are 35 mm., Polaroid*,plate and reflex cameras. ThereIs also a choice of a fully auto-matic 35 mm. camera or a semi-automatic 35 mm., and Polaroid.

Let U3 send you furihtf Informtlfon

M32PATHOLUX

• Polaroid Ittha rtoMtrtd trada mark of thmPolaroid Corporation

(iii)

Details of

Advertisement

rates and data

for Journal of

Cell Science

may be obtained

from:

The Publications Dept.,

Cambridge University Press,

Bentley House,

200 Euston Road,

London, N.W.1

ELECTRONMICROSCOPISTS!

We can now offerover 70 chemicals for Electron Microscopy

from stock. Including:

ACCELERATORSFIXATIVESPOLYMERSRESINS

CURING AGENTSCATALYSTSEPOXIDES'STAINS'

UNSATURATED MONOMERSAlso:MAXTAFORM GRIDS GRID BOXESGELATIN CAPSULES BEEM CAPSULESWATCHMAKERS BOW-SPRING

FORCEPS SCISSORS

Literature giving full detailsof this new service from GURRS is

available on request

GEORGE T. GURR LTD.136-144, New Kinf'i Road, London, S.W.6

T«l: RENown 5482/4Cblei: MICROSTA1N. London, S.W.6

obtain better sections with

JUNGMICROTOME KNIVES

• Will fit any microtomeBeck, Cambridge, Jung, Leitz,M.S.E., Reichert, Spencer, etc.

• A knife for anysectioning technique

* Will section the hardestobjects

* Easier and quickerto hone

Our catalogue containing fulldetails of our extensive rangeof knives, stropping devices,honing stones, and an articleon the 'Correct cutting andsharpening angle' will be senton request if you quote

CS/55/K

GLEN CRESTONThe Red House,37 The Broadway,Stanmoro, Middlesex.Telephone: 01-954 4218

(iv)

'MICHROME'STAINS AND REAGENTS FOR MICROSCOPY, ETC.

Over 3,000 currently available, including:

Alcian Blue Giemsa Stain OptoilAquamount Indophenol Pararosaniline ChlorideAstrazone Pink Janus Green PeptonesAuramine, O Jenner Stain Rosolic AcidAurantia Laktoseal Sudan BlackAzur, A, B, and C Leishman Stain Sun Yellow GAzur B Bromide Lipase ThiofluoresceinCelloidin Lipid Crimson Trifalgic AcidChlorazol Black E Luxol Fast Blue Trypsin

* Chromosome Red Matamount UreaseClearmount Methyl Green Violamine 3 BCristalite Naphthyl Phosphates Wright's StainCytase Nile Blue

DPX Mountant OPTOIL: synthetic, non-sticky immersion oil.FLUORMOUNT & FLUOROIL: mountant and immersion oil for

fluorescence microscopy• Details on application.

fFALG STAIN(B.T. Squires' modification)—A new diagnostic agent with a variety of indications including:Protein-Malnutrition; General State of health in Man and animals; malingering, etc.

t Information leaflet A2 availablo gratis on request.

JRHODANILE BLUEAn entirely new type of basic dye conceived and synthesized for the first time by Dr EdwardGurr with the object of providing a new research tool. This unique polychrome dye, whichpossesses interesting and unusual properties, has a number of very useful applications both inpolarized and ordinary light microscopy; for example, it provides a distinctive stain for fibrin.

t Information leaflet Al available gratis on request.

§PAPANICOLAOU STAINS, ETC.For cytologies! screening of smears for malignancy, etc

i Information leaflet A 4 on staining methods available gratis on request.

Catalogue available on request.

Recent Books by Edward Gurr'RATIONAL USE OF DYES IN BIOLOGY AND GENERAL

STAINING METHODS' 422 pages royal 8ro., 105/-' STAINING ANIMAL TISSUES' Practical and Theoretical, 631 pages, 95/-

'ENCYCLOPAEDIA OF MICROSCOPIC STAINS'500 pages royal 8vo., price 105/-

Edward Gurr Ltd.42 UPPER RICHMOND ROAD WEST, LONDON, S.W.14

Cables: Mlcromlabs, London. Telephone: 01-876—8228/9.

Uniform High Quality and Reliability.

(v)

"History in the Breaking!"In 1950 the first glass knife for ultramlcro-tomy was made, by methods which wererather unconventional and did not exactlyguarantee reproduclbillty, by breaking abottle.

In 1964 glass was still being broken tomake knives for ultramlcrotomy but LKB hadproduced the KnifeMaker. The KnlfeMakerrevolutionized the production of glass knivesfor ultramlcrotomy by making it possible toproduce knives easily, quickly, with a choiceof predictable edge angle, economically, andwith reproduclbillty.

Now In 1966, LKB has once again madehistory In breaking glass for knives, by in-troducing the unique Damping Device thatadds even greater sensitivity (and moreworking edge per knife), to an Instrumentthat has proved its reliability In ultramicro-tomy laboratories and research establish-ments throughout the world.

Don't have a smashing timel Write todayfor further information on the KnlfeMakerwith the unique Damping Device that willgive you a third more usable edge on yourknives than you have ever had beforel

LKB INSTRUMENTS LTD. • LKB HOUSE, 137 ANERLEY ROAD • LONDON S.E. 20

(LKB) OTHCTHEADQUARTERSFOR BALESAND SERVICE

SWEDENLKB-Produkttr ABBox 70,Stockholm—Bromra* 1

USA

LKB IitttruiTrfnta Ino.12221 PuWtwn DHv«,Rockvilla Md 206G2

NETHERLANDSLKB-Produknn N V. LKB Imnanftt A78

A t d 34Z t s ,Th« Hasu*

(vi)

for accurate sectioning of difficult specimens

Everv difficult and non-homogeneous tissues are cut into flawless sections with theJUNG 1120 rotary microtome. It is accurate because its massive object holder is locatedin the centre of two substantial slideways which directly absorb the thrust createdduring cutting. It is reliable because its robust feed mechanism is housed in an enclosedcylinder which protects it from dust, moisture and accidental damage. Serial sectionsas large as 40 x 50 mm can be cut; the automatic object feed is adjustable over a widerange from 1 to 40 microns; built-in section counter and knife guard are standardequipment, whilst a freezing stage and drive by variable speed motor are optional.

GLEN CRESTONThe Red House, 37 The Broadway, Stanmore, Middlesex.Telephone: 01-954 4218for further particulars and prices please quote Key No. CS/54IJR

(vii)

CAMBRIDGEJournal of theMarine Biological Association of the U.K.Edited by J. E. SMITH

Volume 47, Number 1. February 1967

T. E. THOMPSON. Direct development in a nudibranch, Cadlina laevis, with a discussion ofdevelopmental processes in Opisthobranchia.E. D. WOOD, F. A. J. ARMSTRONG and F. A. RICHARDS. Determination of nitrate in sea water bycadmium-copper reduction to nitrite.WELTON L. LEE, BARBARA M. GILCHRIST and R. PHILLIPS DALES. Carotenoid pigments inSabella penicillus.F. G. T. HOLLIDAY and M. PATTIE JONES. Some effects of salinity on the developing eggs andlarvae of the plaice (Plewonectes plalessa).J. H. WICKSTEAD. Branchiostoma lanceolatum larvae: some experiments on the effect of thiouracilon metamorphosis.P. SPENCER DA VIES. Physiological ecology of Patella. II. Effect of environmental acclimation onthe metabolic rate.J. DAVID GEORGE. Cryptic polymorphism in the cirratulid polychaete, Cirriformia tentaculata,A. J. SOUTHWARD. Recent changes in abundance of intertidal barnacles in south-west England:a possible effect of climatic deterioration.R. N. GIBSON. Experiments on the tidal rhythm of Blennius pholis.E. D. S. CORNER and B. S. NEWELL. On the nutrition and metabolism of zooplankton. IV. Theforms of nitrogen excreted by Calanus.R. H. HEDLEY and J. ST J. WAKEFIELD. Clone culture studies of a new rosalinid foraminifer fromPlymouth, England and Wellington, New Zealand.JOHN D. FISH. The biology of Cucumaria elongata (Echinodermata: Holothuroidea).CLYDE MANWELL, C. M. ANN BAKER, P. A. ASHTON and E. D. S. CORNER. Biochemical differencesbetween CaJanus finmarchicus and C. helgolandicus. Esterases, malate and triosephosphate dehydro-genases, aldolase, 'peptidases', and other enzymes.H. BARNES and R. EVENS. Studies in the biochemistry of cirripede eggs. HI. Changes in the amino-acid composition during development of Balanus balanoides and B. balanus.E. J. DENTON, J. B. GILPIN-BROWN and J. V. HOWARTH. On the buoyancy of Spirula spirula.D. H. CUSHING. The grouping of herring populations.T. H. J. GILMOUR. The defensive adaptations of Lima hiaru (Mollusca, Bivalvia).A. D. BONEY and E. B. WHITE. Observations on an endozoic red alga.G. RUSSELL. The genus Ectocarpus in Britain, n . The free-living forms.ABSTRACTS OF MEMOIRS.

£5 Net. Annual Subscription (5 issues) £14

Published for the Marine Biological Association

CAMBRIDGE UNIVERSITY PRESS

(viii)

CAMBRIDGEThe Journal of Experimental Biology

Edited by Y. B. WIGGLES WORTH & A. J. RAMSEY

Volume 46, Number 2. April 1967

JOHANSEN, KJELL and HANSON, DAVID. Hepatic vein sphincters in elasmobranchs and theirsignificance in controlling hepatic blood flow.JOHANSEN, KJELL and LENFANT, CLAUDE. Respiratory function in the South American lungfish,Lepidosiren paradoxa (Fitz).PENNYCUICK, C. J. The strength of the pigeon's wing bones in relation to their function.HARVEY, W. R., HASKELL, J. A. and ZERAHN, K. Active transport of potassium and oxygen con-sumption in the isolated midgut of Hyalophora cecropia.ATWOOD, H. L. and WIERSMA, C. A. G. Command interneurons in the crayfish central nervoussystem.DEL CASTILLO, J., DE MELLO, W. C. and MORALES, T. The initiation of action potentials in thesomatic musculature of Ascarts lumbricoides.BRYAN, G. W. Zinc regulation in the freshwater crayfish (including some comparative copperanalyses).HOLETON, G. F. and RANDALL, D. J. Changes in blood pressure in the rainbow trout duringhypoxia.STEVENS, E. DON and RANDALL, D. J. Changes in blood pressure, heart rate and breathing rateduring moderate swimming activity in rainbow trout.HOLETON, G. F. and RANDALL, D. J. The effect of hypoxia upon the partial pressure of gases inthe blood and water afferent and efferent to the gills of rainbow troutSTEVENS, E. DON and RANDALL, D. J. Changes of gas concentrations in blood and water duringmoderate swimming activity in rainbow trout.RANDALL, D. J., HOLETON, G. F. and STEVENS, E. DON. The exchange of oxygen and carbondioxide across the gills of rainbow trout.MILLER, P. L. The derivation of the motor command to the spiracles of the locust.SATCHELL, G. H. and JONES, M. P. The function of the conus arteriosus in the Port Jacksonshark, Heterodontus portusjacksoni.VOGEL, STEVEN. Flight in Drosophila. II. Variations in stroke parameters and wing contour.

30s. Net. Annual Subscription (6 issues) £8

Published for the Company of Biologists, Limited

CAMBRIDGE UNIVERSITY PRESS

Ox)

Focus on UltramicrotomyWe at 1KB want to focus your attention on the solution,not on the problem-get your problems In focus and solvethem the LKB-way.

LKB ULTROTOME III SOLVESPROBLEM OF "COMPRESSION""Compression" (not to be confused with "chatter"), Is adeformation of the section caused during cutting wherebythe section length becomes shorter than the pyramidheight. Compression deforms the specimen ultrastructure,makes direct measurement of micrographs Inaccurate(without corrections), and morphology misleading.

Design of LKB optical system (viewing at 45° Incidentangle), checks compression and estimates correction fac-tor where It has occured. Low speeds eliminate or mini-mize compression; the LKB ultramlcrotome can produceusable sections at 0.5 and 0.2 mm/sec, because there areNO VIBRATIONS.

The first otan LKB-serles dealing with problems In ultra-microtomy.

Answer to many more problems provided by uniquedesign features:Cutting speeds: Widest range available, 20-0.1 mm/sec.Optical system: Most flexible-pyramid tlp/knlfe edge al-ways central.

Speclmen/Knlfe Holders: Complete freedom of structureorientation.Fe«d: Unear thermal. Two Independent manual mechanicalsystems.

Focused Lamps: Provides evalaution of knife edge qualityand angle.Section Collector: Simplifies picking up sections end ma-nipulation of grids.Diamond Knife sectioning: Gravity powered specimenarm prevents damage.Machine Trimming: Fast procedure and superior pyramids.

For the complete story write today for our fully Illustratedtwo-colour brochure describing In detail the LKB Ultra-mlcrotome-ULTROTOME III.

LKB INSTRUMENTS LTD. • LKB HOUSE, 137 ANEBLEY ROAD • LONDON 8.E. 20

OTHERHEADQUARTERS:ron SALESAND SERVICE

SWEDENLKB-Produkur ABBox 76,

LKfi IrutnjnttflU Inc.12221 Ptrfclawn Drivo,Rodnill* Md2M52

NETHERLANDSLKB-Produfctin N.V.z . ik jm as,

H

A/a84

00

INFORMATION FOR CONTRIBUTORS

1 Manuscripts should be sent to The Editors,Journal of Cell Science, Department of Zoology,Cambridge, England.

2 Manuscripts must be typewritten, in doublespacing throughout (including tables, references andlegends). Each table should be typed on a separatesheet. Legends to figures should be typed in asingle series and placed at the end of the manuscript.Papers must be fully corrected by the author, and acharge will be made for excessive alteration in proof.

3 A short title of not more than 40 characters, foruse as page headings, should be supplied if the fulltitle is longer than this.

4 Manuscripts must contain a Summary of notmore than 500 words, placed immediately afterthe title page. Contributors should also send threecopies of an Abstract for distribution to abstractingjournals. The abstract must be not more than 100words long and should be headed by the author'sname and address and the title of the paper. Bothsummary and abstract must be intelligible withoutreference to the main text.

5 The list of References must be given inalphabetical order of authors' names. The titles ofjournals should be abbreviated in accordance withthe World List ojScientific Periodicals,^ ed. (1963).The following style is used:

BARNICOT, N. A. & HUXLEY, H. E. (1965).

Electron microscope observations on mitoticchromosomes. Q. jfl microsc. Set. 106, 197-214.

MAZIA, D. (1961). Mitosis and the physiology ofcell division. In The Cell, vol. 3 (ed. J. Brachet& A. E. Mirsky), pp. 77-412. New York andLondon: Academic Press.

Citations in the text are given in the following form:Jones & Smith (i960) or (Jones & Smith, i960).Where there are more than two authors the firstcitation should include all the names and subsequent

citations should be in the form (Jones et al. i960).Where more than one paper by the same authors)have been published in the same year they are citedas Jones (1960a), Jones (19606) etc.

6 Text figures should preferably be drawn abouttwice final size; very large drawings should beavoided. Photographic reproductions of drawingscannot always be satisfactorily reproduced. Themaximum printed size of a drawing is 5 in. by8 in. Lettering will be inserted by the printers andshould be indicated on drawings in faint bluepencil or on a tracing-paper overlay. It should be inlower case, and abbreviations should not be used ifthere is space for complete words.

7 Photographs should preferably be submitted thesame size as they are to appear. The maximum areafor a plate is 5J in. by 8i in. Where several photo-graphs make up a plate they should be accuratelymounted on one sheet of cardboard. Irregularlyshaped photographs or plates should be avoidedwherever possible. Lettering on plates will be in-serted by the printers and should be indicated eitheron a duplicate, marked set of prints or on a tracing-paper overlay bearing accurately marked outlinesof the objects indicated. Authors may be asked tocontribute to the cost of plates in excess of four.

8 Text figures and photographs should benumbered in a single series, all text figures pre-ceding the photographs. Each individual drawingor photograph should be numbered separately(Fig. 1, Fig. 2 and soon).

9 Where appropriate the magnifications ofillustrations should be indicated by scales drawn onthem. Magnifications may also be stated in thelegends.

10 Authors will receive 50 offprints free of chargeand may order additional copies when proofs arereturned.