p rotein machines
DESCRIPTION
p rotein machines. A proteasome chops proteins until they are 7÷8 amino acids long. p rotein machines. A proteasome chops proteins until they are 3 to 23 aminoacids long. p rotein machines. A proteasome chops proteins until they are 3 to 23 aminoacids long. p rotein machines. - PowerPoint PPT PresentationTRANSCRIPT
protein machines
A proteasome chops proteins until they are 7÷8 amino acids long
The lysosome is a membranous bag which contains hydrolytic enzymes that are used to digest macromolecules. The lysosome contains over 40 enzymes, some of which are the proteases, nucleases, and phopholipases. These enzymes optimally work at a pH of 5 (acidic), so should these enzymes leak out they would cause minimal damage to the cytoplasm
protein machines
A proteasome chops proteins until they are 3 to 23 aminoacids long
The lysosome is a membranous bag which contains hydrolytic enzymes that are used to digest macromolecules. The lysosome contains over 40 enzymes, some of which are the proteases, nucleases, and phopholipases. These enzymes optimally work at a pH of 5 (acidic), so should these enzymes leak out they would cause minimal damage to the cytoplasm
protein machines
A proteasome chops proteins until they are 3 to 23 aminoacids long
The lysosome is a membranous bag which contains hydrolytic enzymes that are used to digest macromolecules. The lysosome contains over 40 enzymes, some of which are the proteases, nucleases, and phopholipases. These enzymes optimally work at a pH of 5 (acidic), so should these enzymes leak out they would cause minimal damage to the cytoplasm
protein machines
Engineered protein machines can be embedded in synthetic nanomembranes that may one day break down cellulose more efficiently or produce hydrogen for fuel cells
Overview of two-hybrid assay, checking for interactions between two proteins, called here Bait and Prey.A. Gal4 transcription factor gene produces two domain protein (BD and AD), which is essential for transcription of the reporter gene (LacZ).B,C. Two fusion proteins are prepared: Gal4BD+Bait and Gal4AD+Prey. None of them is usually sufficient to initiate the transcription (of the reporter gene) alone.D. When both fusion proteins are produced and Bait part of the first interact with Prey part of the second, transcription of the reporter gene occurs.
Engineered Saccharomyces cerevisiae (no Gal4 gene)
an intensely blue productwhich is insoluble
