paper 1: bleiholder c., dupuis n f., wyttenbach t. & bowers, m.t. ion mobility-mass spectrometry...
TRANSCRIPT
![Page 1: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/1.jpg)
1
Protein misfolding characterization by ion mobility-mass spectrometry (IM-MS)
Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly to b- sheet in amyloid fibril formation. Nat. Chem. 3, 172-177 (2010)
Paper 2: Gessel M.M., Wu C., Li H., Nitan G., Shea J. & Bowers, M.T. A (39-42) b Modulates A b Oligomerization but not Fibril Formation. Biochemistry. 51, 108-177 (2011)
Presentation by: Mahati MokkaralaDate of Presentation: 12/4/12
![Page 2: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/2.jpg)
2
Mass Spectrometry- What is it?
Effective method for determining compound chemical structure, protein modification patterns, interactions, etc.
Protein Sample/ (can be liquid, other states)
Ionization- Hard or Soft methods (conversion to gaseous state) Ex: for proteins, ESI (nano), MALDI with lasers
Mass Spectrometer machine- mass analyzer/detector. Example: Time of Flight Mass Spectrometer (TOF), Quadrupole Mass Spectrometer (QMS)-Detects m/z z/n of various ion fragments
![Page 3: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/3.jpg)
3
Ion mobility-mass spectrometry (IM-MS)
Ion mobility devices separate (peptide sequence) ions based on particle mobility, shape, charge
Easily pair ion mobility with mass spectra and ionization devices [5]
![Page 4: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/4.jpg)
4
Physics of ion mobility in IM-MS
• Peptide ion fragments enter chamber filled with gas (buffer gas, chiral selectivity element, etc)• Ion mobility delayed- ‘friction’ collisions with gas molecules- propelled by electric field (Image from source [2])
![Page 5: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/5.jpg)
5
IM-MS Classification
Linear Drift Time (LDT) Mass Spectrometry- ‘easier’ calculation correlation between collision cross section and drift time for ions
Traveling Wave Ion Guide (TWIG) IM-MS
Field Asymmetric Ion Mobility Mass Spectrometry (FAIM) [4].
![Page 6: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/6.jpg)
6
Linear Drift Time Mass Spectrometry (LDT- Mass Spec.)
LDT gas tube- with weak electric field- constant drift velocity
Can average collisions to get the collision cross section
Advantages: high resolution, easier to quantify degree of ion separation
Disadvantages: low ‘drift cycle’ need to constantly introduce a pulse of ions- can promote wasting of a large portion of sample source [4]
Image from source [4] (see works cited), in source reprinted by permission from source [20] in paper
![Page 7: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/7.jpg)
7
LDT IM-MS Key Equations
(K naught) Reduced mobility ~ 1/ W( = W Collision Cross section Image from source [4]
How to calculate K naught? K simplified, related to drift time P- pressure, V- voltage, linear relationship Image from source [2]
![Page 8: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/8.jpg)
8
LDT IM-MS Key Equations cont.
Bowers et al. Paper 2, summarizes key relationship between (s collision cross-section) and drift time
q = ion charge, T = temperature, m= reduced mass, N = He/gas number density, l = drift cell length
![Page 9: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/9.jpg)
9
Paper 1 Problem/Results/Discussion
Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly to b- sheet in amyloid fibril formation. Nat. Chem. 3, 172-177 (2010)
![Page 10: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/10.jpg)
10
Problem/Question of Interest
Detection oligomer shifts- tough to characterize due to quick conformational shifts
With IM-MS, could greater determine at oligomer combination (n) globular-b sheet transformation occurs.
![Page 11: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/11.jpg)
11
Protocol- Paper 1
ESI/Quadrople Mass Spec. Image from: http://chemwiki.ucdavis.edu
IM (from source [3]
•amyloid-forming yeast prion protein Sup35 (NNQQNY)•human insulin regions- (VEALYL) •human islet amyloid polypeptide- (SSTNVG)•YGGFL- usually forms an exclusively isotropic not fibril structure
Peptides exposed to following apparatus:
And then
![Page 12: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/12.jpg)
12
Cont. Protocol Paper 1
IM (time of delay) – calculate s for each oligomer (size n)
Compare collision cross section per oligomer number (n) with theoretical (s n) for fibril/isotropic growth
Isotropic Growth formula: Fibril Growth formula:
![Page 13: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/13.jpg)
13
Results- Paper 1 –ESI- Quadrupole Mass Spectra
Mass Spectra- indicates oligomerization due to large n/z observed for two peptides- YGGFL, VEALYL (one isotropic growth control, other fibril
![Page 14: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/14.jpg)
14
Results- Paper 1- Supplementary Figures- Example of IM-MS ATD
Shows sample ATD intensity captures by IM-MS for the NNQQNY peptide; broad peaks- correlate to multiple oligomer combination states- use average drift time for calculations
![Page 15: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/15.jpg)
15
Results-Paper 1
Can clearly correlate experimental collision cross section per each oligomer combination with calculated theoretical (s n)
Top: for YGGFLSecond: for NNQQNY
![Page 16: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/16.jpg)
16
Results- Paper 1Experimental data and proposed oligomerization for peptide VEALYL (c )and peptide SSTNVG (d )
Indicates peptide (c ) –initiates with single strand fibril before at n =5 switching to the zipper form
Peptide (d)- isotropic until n = 12/14, consists of both zipper and isotropic form
![Page 17: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/17.jpg)
17
Results- Paper 1 Physical Verification
Verification of fibril formation at specified oligomer (n) verified by AFM visualization of each protein mixture sample
![Page 18: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/18.jpg)
18
Conclusions- Paper 1
With IMS-MS, now cab follow through peptide self-assembly step by step from an oligomer of 1 for given peptide fragment
Stresses importance of the IMS-MS technique can learn more on at what state oligomer- b fibril transformation occurs
Very relevant for greater study of amyloid b caused diseases
![Page 19: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/19.jpg)
19
Paper 2 Problem/Results/Discussion
Paper 2: Gessel M.M., Wu C., Li H., Nitan G., Shea J. & Bowers, M.T. A (39-42) b Modulates A bOligomerization but not Fibril Formation. Biochemistry. 51, 108-177 (2011)
![Page 20: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/20.jpg)
20
Paper 2 – Problem/Question of Interest
Mechanism of (39-42) Ab binding to 42Ab or 40 Ab tough to experimentally
verify via X ray crystollagraphy or NMR IM-MS and molecular dynamic
simulations as well as ThT assays- further verify (39-42) Ab interactions with 42 Ab and 40 Ab
Why important?: CTF A (39-42) bknown to inhibit A b toxicity
![Page 21: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/21.jpg)
21
Paper 2- Methods/Techniques IM- (nanospray) ESI- quadrople
mass spectra- oligomer disassociation due to (39-42) (Ab CTF)
ThT fluorescence assay- does (39-Ab42)influence/limit fibril formation?
Modeling software- AMBER force field simulation, SHAKE- verify possible binding/structure (39-42) Ab with Abpeptide
![Page 22: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/22.jpg)
22
Paper 2- ESI-Mass Spec. Results
Results- Definite difference in mass spectra peaks between both spectra a- Amyloid particle alone, b- Amyloid plus 1:5 CTF added
-Key peaks to focus on in b figure: z/n = -5/2 peak – one CTF/dimer
z/n = -3, 1 or 2 CTF bound to single oligomer (A 42) b
![Page 23: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/23.jpg)
23
Paper 2 – IM- MS results n/z = -5/2 m/z = ~ 1800 for dimer peak- does CTF prevent dodecamers? Ans: Yes.
n/z = -5/2 for A 42 bparticle – Does CTF reverse A baggregation? Ans: Yes
Incubation of select amyloid dimer peaks for 2 hours prior to exposure to CTF
Prevention of dodecamers, decamers requires high (1:5) concentration CTF
![Page 24: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/24.jpg)
24
Paper 2- cont. IM-MS results
Question: Does CTF bind to tetramers, hexamers, dimers of amyloid 42? bAns: Yes
Expose m/z = 1884 peak with bound CTF to dimers to IM-MS indicate definite cross sections for dimer, tetramer, hexamer
![Page 25: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/25.jpg)
25
Paper 2- IM-MS for 40- Ab CTF Interactions?
Similar experiment repeated for the A 40 b peptide- as above, observe distinctive peaks z/n = -4, -3 for one or two CTF- binding to single oligomer
One dimer-CTF species identified
IM-MS indicates- no shift oligomer size with CTF, same as peptide
CTF- interacts with A 40b
![Page 26: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/26.jpg)
26
Paper 2: Results, ThT and Toxicity Studies
Both CTF plus A 40/ 42 b Abpeptides with MTT assay-PC12 cells promotes cell viability –importance of breaking toxic oligomer aggregates
ThT fluorescence- EM microscope visualization-Fluorescence increase-fibrils--Oligomers eventually to fibrils even with CTF
![Page 27: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/27.jpg)
27
Paper 2 Results: Modeling
Observe structure if CTF binds to Ab more than 20 times, adds to being in a bound state, etc
Calculate cross sections of structures (long collision integral)
Compare structures to Mass Spec experimental data
Observe: CTF fragments bind: N, C terminus, internal regions via van der Walls interactions of 42 Ab peptide.
![Page 28: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/28.jpg)
28
Paper 2 Conclusions:
With IMS-MS techniques:. CTF binds (Van der Waals) with monomeric, 2,4,6 Amyloid b
42 particles CTF disassociates dodecamers into non toxic oligomers 40Ab – binds with two CTF via electrostatic interaction, no
disaggregation oligomers CTF binding- C, N terminus, internal structures Amyloid b 42
![Page 29: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/29.jpg)
29
Works Cited
[1] Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly to b- sheet in amyloid fibril formation. Nat. Chem. 3, 172-177 (2010)
[2] “Theories and Analysis.” The Bower’s Group UC Santa Barbara. <http://bowers.chem.ucsb.edu/theory_analysis/> . Accessed: December 3, 2012.
[3] Gessel M.M., Wu C., Li H., Nitan G., Shea J. & Bowers, M.T. A (39-42) b Modulates A b Oligomerization but not Fibril Formation. Biochemistry. 51, 108-177 (2011)
[4] Harvey S.R. MacPhee C.E., Barran P.E. Ion mobility mass spectrometry for peptide analysis. Methods. 54(4), 454-461 (2011)
[5] Kanu A.B., Dwivedi P., Tam M., Matz L., Hill H.H., Ion mobility- mass spectrometry. Journal of Mass Spectrometry. 43, 1-22 (2008)
![Page 30: Paper 1: Bleiholder C., Dupuis N F., Wyttenbach T. & Bowers, M.T. Ion mobility-mass spectrometry reveals a conformational conversion from random assembly](https://reader036.vdocument.in/reader036/viewer/2022081516/551aa0b055034656628b45f0/html5/thumbnails/30.jpg)
30
Questions?