Perspectivas en el uso de Hongos Entomopatogenos para

Control Biológico deGarrapatas

Rodrigo A Zambrano MVZ Esp MSc

Mamíferos, Aves, reptiles y anfibios

Orden: Ácaros

• Argasidae (soft ticks)

• Nuttalliellidae(restricted to Southern Africa),

• Ixodidae (hard ticks).

Tick borne diseases of humans

Ticks and tick-borne diseases: One Health perspective Filipe Dantas-Torres, Bruno B. Chomel and Domenico Otranto, Trends in Parasitology, October 2012, Vol. 28, No. 10

Patógenos Asociados a Garrapatas

Ticks and tick-borne diseases: One Health perspective Filipe Dantas-Torres, Bruno B. Chomel and Domenico Otranto, Trends in Parasitology, October 2012, Vol. 28, No. 10

Patógenos Asociados a Garrapatas

Infestación en Humanos

Ticks and tick-borne diseases: One Health perspective , Filipe Dantas-Torres, Bruno B. Chomel and Domenico Otranto, Trends in Parasitology, October 2012, Vol. 28, No. 10

Estrategias de Control

• Hospedador Agente y Ambiente (T°)

• Distintos comportamientos , preferencias , ciclos de vida y susceptibilidad a Hongos

• Adaptacion a la garrapatablanco y al ambiente propiode la garrapata

Metarhizium anisopliae y Beauveria bassiana

Comparative studies on the invasion of cattle ticks (Rhipicephalus (Boophilus)microplus) and sheep blowflies (Lucilia cuprina) by Metarhizium anisopliae (Sorokin)D.M. Leemon a,⇑, N.N. Jonsson, Journal of Invertebrate Pathology 109 (2012) 248–259

Revisiones

• Samish and Rehacek(1999) Hongos y Nematodos

• Samish et al. (2004). Necesidad de nuevascepas de nematodos

• Fernandes and Bittencourt (2008) Sur America

Conidias en suspension

Effectiveness of Beauveria bassiana sensu lato strains for biological controlagainst Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) in ChinaMing Sun a,b, Qiaoyun Ren a, Guiquan Guan a, Yufeng Li b, Xueqing Han c,Chao Ma b, Hong Yin a,⁎, Jianxun Luo Parasitology International 62 (2013) 412–415

Diferentes concentraciones y varias aplicaciones

Effectiveness of Beauveria bassiana sensu lato strains for biological controlagainst Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) in ChinaMing Sun a,b, Qiaoyun Ren a, Guiquan Guan a, Yufeng Li b, Xueqing Han c,Chao Ma b, Hong Yin a,⁎, Jianxun Luo Parasitology International 62 (2013) 412–415

Condiciones de Campo

High temperature, strong solar ultraviolet (UV) radiation and desiccation, often severely reducing the persistence of fungal conidia in the field; and, thereby, reduce the efficacy of fungal biological control products

Rangel et al., 2004; Braga et al., 2002

Entomopatogenicidad Diferencial

• Natural UV tolerance

• Thermotolerance and cold activity

• Genotypes An intensive search for promising fungal biological control agents of ticks, particularlyRhipicephalus microplus Éverton K.K. Fernandesa,b Isabele C. Angeloa, Drauzio E.N. Rangel, Veterinary Parasitology 182 (2011) 307– 318

Beauveria bassiana y Metarhiziumanisopliae

Laboratory evaluation of virulence of Chinese Beauveria bassiana and Metarhiziumanisopliae isolates to engorged female Rhipicephalus (Boophilus) microplus ticksQiaoyun Ren, Zhijie Liu, Biological Control 63 (2012) 98–101

Beauveria bassiana y Metarhiziumanisopliae

Differential susceptibility of Amblyomma maculatum and Amblyomma americanum (Acari:Ixodidea) to the entomopathogenic fungiBeauveria bassiana and Metarhizium anisopliae Brett H. Kirkland, Eun-Min Cho, Nemat O. Keyhani, Biological Control 31 (2004) 414–421

Microclimas

• Interna de las orejas

• Perianal

• Establos

Monteiro et al., 2003, , Souza et al.,2009, Soulsby, 1987

Beauveria bassiana contra Psoroptes spp

In vitro entomopathogenic activity of Beauveria bassiana against Psoroptes spp. (Acari: Psoroptidae) M. Lekimme *, B. Mignon, S. Tombeux,, Veterinary Parasitology 139 2006) 196–202

Lecanicillium lecanii

Efficiency of Lecanicillium lecanii to control the tick Rhipicephalus Microplus Isabele C. Angelo, Éverton K.K. Fernandes,, Veterinary Parasitology 172 (2010) 317–322

Lecanicillium lecanii

Efficiency of Lecanicillium lecanii to control the tick Rhipicephalus Microplus Isabele C. Angelo, Éverton K.K. Fernandes,, Veterinary Parasitology 172 (2010) 317–322

Paecilomyces fumosoroseus

Virulence of Mexican isolates of entomopathogenic fungi (Hypocreales: Clavicipitaceae) uponRhipicephalus = Boophilus microplus (Acari: Ixodidae) larvae and the efficacy of conidia formulations to reduce larval tick density under field conditions, C.A. Ángel-Sahagúna, R. Lezama-Gutiérrez, VeterinaryParasitology 170 (2010) 278–286

Garrapaticidas

Efectos de la acumulación de cipermetrina en hígado, grasa peri renal y músculos de bovinos consumiendo pastos

fumigados con sobredosis del piretroide

• Walther Hernandez, John Bustamante. ENICIP 2013.

• La utilización indiscriminada de cipermetrinas que son consumidas por los bovinos representa una grave amenaza para la salud publica.

Luis Orlando Castro PhD

LOOCC International Corporation

Trichoderma harzianum Trichoderma virensMetarhizium anisopliaeBacillus thuringiensisBacillus subtilisBacillus polymyxaBeauveria bassiana Entrophospora colombiana Verticillium lecaniiGlomus intraradicesPaecilomyces famosoroseus

Hongos En la Mezcla

Mecanismo de Acción

Comparative studies on the invasion of cattle ticks (Rhipicephalus (Boophilus)microplus) and sheep blowflies (Lucilia cuprina) by Metarhizium anisopliae (Sorokin)D.M. Leemon a,⇑, N.N. Jonsson, Journal of Invertebrate Pathology 109 (2012) 248–259

Mecanismo de Acción

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Gracias