pGLO

Bacterial Genetics

E. Coli structure

E. Coli structure

E. Coli structure

E. Coli structure

E. Coli structure

E. Coli structure

E. Coli structure

E. Coli structure

Plasmid

• Additional DNA found in some bacteria

• Small loop• Carries one or more

genes• Can be transferred

from one bacteria to another.

pGLO plasmid (original form)

• ori- point of origin for DNA replication

• araC- gene for repressor protein in arabinose operon

• araB, A and D- gene for arabinose metabolism

ara B, ara A & ara D

pGLO plasmid (engineered form)

• Gene for arabinose metabolism (ara BAD) has been replaced with GFP.

• Amipicillin resistance has been added (bla)