Induced hyperpigmentation from mutated Gallus gallus

Joanly Rivera Ortiz

Research Proposal

RISE Summer Program 2015

BIOL3009

Genetic Engineering

Manipulation of genes. Fribromelanosis expressions.

"abnormal accumulation of the dark pigment melanin in the dermis and connective tissue formations" (Yablanski, T.)

Recreate the mutation in other domestic animals.

There are three breeds of Gallus gallus with fibromelanosis Ayam Cemani (originating from Indonesia) Svart Höna / Swedish Black Chicken

(descendant of Ayam Cemani) Tuzo Argentina (variation from the Aseel,

native to India)

Hypothesis

GM offspring expresses

fibromelanosis

The modification will

affect other

characteristics

Variables

Independent variable:

Mutated gene from

Gallus gallus

Dependent variable:

Offspring’s genes

Objectives

Anatomical comparison

Decode DNA

Isolate gene

Insert gene into a retrovirus - GeneDetect®

‘Infect’ receptor In vitro fertilization

8 eggs

10 mothers

InbreedingFirst generationSecond generation

Graphics by Jeffery Lim, Copyright @ 2007 Singapore Press Holdings. All rights reserved.

Analyze GM specimen

Anatomy

Physical condition

Intelligence

Benefits

Military purposes Completely black Belgian Shepherds

Pet/exotic animals lovers Any type of animal with this pigmentation would

have great value on the market.

Black pigs resist heat better Could survive in Africa where food is in scarcity

Potential Pitfalls

High percentage of immature eggs

Unexpected mutations

Miscarriages

Sicken offspring

Works citedEvaluation of AAV-Mediated Expression of Chop2-GFP in the

Marmoset Retina. (2010, October). Retrieved June 1, 2015.

Palmer, J. (2009, May 27). Glowing monkeys 'to aid research' Retrieved June 30, 2015.

rAVETM custom AAV2 serotype viral vectors. Retrieved July 5, 2015, from http://www.genedetect.com/rave.htm#ravesupply

What is genetic engineering? (2014, December 22). Retrieved July 2, 2015, from http://www.yourgenome.org/facts/what-is-genetic- engineering

Yablanski, T. (Ed.). (2013). Fibromelanosis in domestic chickens. Agricultural Science and Technology, 5(3), 239-240.

Retrieved June 16, 2015.

Questions?

Thank you for your attention