PRESENT

THE 8th INTERNATIONAL FRUCTAN SYMPOSIUM JUNE 26th TO JULY 1st 2016

OAXACA, MEXICO

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INTERNATIONAL SCIENTIFIC COMMITTEE

De Gara Laura (Universit Campus Bio-Medico di Roma, Italy) Gallagher Joe (IBERS, Aberystwyth, UK)

Greiner Steffen (Heidelberg Institute for Plant Sciences, Germany) Gupta Anil (Punjab Agricultural University, India)

Hernndez Lzaro (Centro de Ingeniera Gentica y Biotecnologa CIGB, Cuba) Livingston David (US Deparment of Agriculture, North Carolina State, USA)

Lpez Mercedes G. (CINVESTAV-Centro de Investigacin y de Estudios Avanzados-IPN, Mexico)

Lpez-Mungua Agustn (IBT-Instituto de Biotecnologa de la UNAM Mexico) Praznik Werner (University of Natural Ressources and Applied Life Sciences, Austria)

Prud'homme Marie-Pascale (Universit de Caen Basse-Normandie, France) Rasmussen Susanne (AgResearch Grasslands, Palmerston North, New Zealand)

Shiomi Norio (Rakuno Gakuen University, Japan) Smeekens Sjef (Utrecht University, The Netherlands)

Spangenberg German (Molecular Plant Breeding Research Centre, Australia) Van den Ende Wim (Katholic University Leuven, Belgium)

Yoshida Midori (National Agricultural Research Center of Hokkaido Region, Japan)

LOCAL SCIENTIFIC COMMITTEE

Mercedes G. Lpez (Cinvestav-Irapuato) Agustn Lpez-Mungua (IBT-UNAM)

Erika Mellado-Mojica (CICY) Alicia Huazano-Garca (Cinvestav-Irapuato) Elena Franco-Robles (Cinvestav-Irapuato)

Adelfo Escalante (IBT-UNAM) Patricia A. Santiago-Garca (IPN-CIIDIR-Oaxaca)

LOCAL ORGANIZING COMMITTEE

Mercedes G. Lpez (Cinvestv-Irapuato) Agustn Lpez-Mungua (IBT-UNAM)

Patricia Santiago-Garca (CIIDIR-Oaxaca) Ma. de Jess Alvarado (Cinvestav-Irapuato)

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IMPORTANT NOTES Oral Presentation Times 1. Plenary presentation 60 min 2. Keynote presentation 40 min 3. Invited presentation 30 min 4. Regular presentation 20 min Posters and Booths Posters and booths can be set up on Sunday 26 and Monday 27. They can be taking off from exhibition on Thursday afternoon. Posters size 3 ft. (wide) x 4 ft. (height). Diplomas and Abstracts These two documents (pdf) can be found in the IFS2016 web page. Extracurricular Activities During the Event Dinner and Guelaguetza (Sunday) Coffee Breaks (Monday to Thursday) Lunch (Monday) City Tour (Monday) Snack (Tuesday) Monte Albn VIP (Tuesday) Cheese and Mezcal (Wednesday)

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AGENDA SUNDAY, JUNE 26, 2016 4:00 PM Registration (Lobby Salon Oaxaca 1) 7:00 PM Opening Dinner with a Guelaguetza Show (At Patio los Laureles in the Hotel) MONDAY, JUNE 27, 2016 8:00 AM Registration, Posters and Booths Set up (Lobby

Salon Oaxaca 1) 9:00 AM OPENING REMARKS (Salon Oaxaca 1) Mercedes G. Lpez and Agustn Lpez-Mungua 9:15 AM PLENARY Chair: Agustn Lpez-Mungua

Prof. Norio Shiomi Structural analysis and formation of novel oligosaccharides containing fructose residue(s) from food sources

10:15 AM SESSION I: SYNTHESIS AND CHARACTERIZATION OF FRUCTANS Chair: David Livingston KEYNOTE Erika Mellado-Mojica and Mercedes G. Lpez Agavins: A new fructan alternative with promising biotechnological potential

NOTE: Posters and Booths will be on exhibition from Monday to

Thursday in the Lobby of the Salon Oaxaca 1 COFFEE BREAK 11:00 AM 11:30 AM

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11:30 AM Prof. Werner Praznik, Renate Lppert, and Helmut Viernstein Applications and evaluation of methods for the profile analysis of fructan products from different sources

12:00 PM Jos M. Cruz Rubio, Paola A. Palmeros Surez, and Juan F.

Gmez Leyva A novel method for the characterization and quantitation of fructan based on the DNS method and HPTLC separation

12:20 AM Miguel A. Snchez-Madrigal, Carlos A. Amaya-Guerra, Armando Quintero-Ramos, Juan G. Bez-Gonzlez, Mara A. Nez-Gonzlez, Sandra L. Castillo-Hernndez, and Jos A. Garzn-Tiznado Ultrasound-assisted extraction of fructans from Agave tequilana Weber var. azul at different ultrasound powers and solid-liquid ratios

12:40 PM Orlando D. de la Rosa, Cristbal N. Aguilar, Diana B. Muiz-

Mrquez, Ral Rodrguez, and Juan C. Contreras-Esquivel Evaluation of different agroindustrial by-products enriched with agave syrup for fructooligosaccharides (FOS) production by solid-state fermentation

LUNCH BREAK (At Patio los Laureles) 1:00 - 3:00 PM 3:00 PM Nadezhda Petkova Tr., Manol Hr. Ognyanovb, Ivan G. Ivanova,

and Panteley P. Denev Isolation of inulin from dandelion Roots by microwave and ultrasound-assisted extractions

3:20 PM Prof. Noureddine Benkeblia Can metabolomics be a key to understand fructans metabolism?

4:00 PM SESSION II: BIOCHEMISTRY AND ENZYMOLOGY OF

FRUCTANS Chair: Marie-Pascale PruHomme Prof. June Simpson, Emanuel Avila de Dios, Celso Crtes-Romero, and Alan D. Gmez Vargas Transcriptome mining: the key to unlock the genetics of fructan metabolism in agave species

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4:30 PM Lorrayne V. de Almeida and Moemy Gomes de Moraes Seasonal dynamics of fructans in thickened roots of Ichthyothere

terminalis (Spreng.) blake (Asteraceae) growing in Cerrado Rupestre

5:00 PM OAXACA CITY TOUR

TUESDAY, JUNE 28, 2016 9:00 AM SESSION II: BIOCHEMISTRY AND ENZYMOLOGY OF

FRUCTANS (Continue) Chair: Marie-Pascale PruHomme Prof. Maria A. M. Carvalho The Cerrado Biome: a proper environment for the fructan syndrome

9:40 PM Erika Mellado-Mojica, Luis E. Gonzlez de la Vara, and Mercedes G. Lpez Fructan active enzymes (FAZY) and FOS biosynthesis Agave tequilana Weber blue variety plants of different ages

10:00 AM Sanya S. Steen, Mercedes G. Lpez, and Noureddine Benkeblia Profiling of saccharides -glucose, fructose and sucrose- and short-chain fructooligosaccharides -1-kestose, 1-nystose and DP5- of the photosynthetic leaves and the bulb of onion (Allium cepa) during growing and bulbification

10:20 AM Agustin Lpez-Mungua, A. Avila-Fernandez, M. E. Rodrguez-

Alegra, C. Olvera, and E. Cuevas-Jurez Functional characterization novel -fructofuranosidase from Bifidobacterium longum subsp. infantis ATCC 15697 on structurally

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diverse fructans

10:40 AM Burak A. Erkorkmaz and Ebru Toksoy Oner Cost effective levan production by Halomonas cultures

CRAFT EXHIBITION IN THE HOTEL 11:00 AM 4:00 PM COFFEE BREAK 11:00 AM 12:00 PM 12:00 PM SESSION III: ROLES AND FUNCTIONALITIES OF FRUCTANS IN

PLANTS Chair: Anne Gschaedler

KEYNOTE Prof. Wim Van den Ende Fructan functionality and links to other carbohydrate metabolisms: from fundamentals to applications

12:40 PM Emanuela O. Joaquim, Karina L. Delmondes, Rita de C. L. Figueiredo-Ribeiro, Adriana H. Hayashi, Geraldo A. Melo, and Maria A. M. Carvalho

Fructan metabolism, composition, and localization in underground systems of Gomphrena marginata Seub. (Amaranthaceae) in different phenological phases

LUNCH BREAK 1:00 - 3:00 PM 3:00 PM KEYNOTE

Chair: Anne Gscaedler Prof. Marie-Pascale PrudHomme, Annette Morvan-Bertrand, Marine Zwicke, Catherine Picon-Cochard, and Florence Volaire Fructans, key molecules for grassland species to resist to water stress and climate changes

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3:40 PM Annette Morvan-Bertrand, Frdric Meuriot, Cline Parquet, Didier Goux, Marion Dalmasso, Margot Schusselhuber, Nathalie Noiraud-Romy, Marie-Pascale PrudHomme, Nicolas Elie, Nathalie Desmasures, and Marina Cretenet Phylloplane diversity in fructan- and non-fructan accumulating grassland species and its relationship with carbohydrate metabolism of the host plant

4:00 PM Emanuela O. Joaquim, Taiza M. Silva, Moemy G. Moraes, Rita de C. L. Figueiredo-Ribeiro, and Maria A. M. Carvalho Diversity of reserve carbohydrates in herbaceous species from the Brazilian Cerrado rupestre

4:20 PM 4:40 PM SNACK BEFORE THE TOUR

4:40 PM MONTE ALBAN TOUR

WEDNESDAY, JUNE 29, 2016 9:00 AM SESSION IV: ROLES AND FUNCTIONALITIES OF FRUCTANS

IN MICROORGANISMS KEYNOTE

Chair: Werner Praznik Prof. Lzaro Hernndez, Eulogio Pimentel, Enrique R. Prez, Carmen Menendez, Duniesky Martinez, Alina Sobrino, Ricardo Ramirez, and Yamira Quintero

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1-SSTrec: basis of an integral solution to the main constraints of the current fungal-based technology for short-chain FOS production from sucrose

9:40 AM Frank Jakob, Tharalinee Ua-Ark, and Rudi F. Vogel Microbial fructans as functional food ingredients

10:00 AM Onur Kirtel and Ebru Tosksoy Oner Levansucrase enzyme from moderately halophilic bacterium Halomonas smyrnensis AAD6T

10:20 AM Enrique Raga-Carbajal, Agustn Lpez-Mungua, and Clarita Olvera Insights into the non-processive polymer synthesis catalyzed by Bacillus subtilis levansucrase: Initiators and Intermediates

10:40 AM Flor de M. Garca-Paz, Sandra Morales-Arrieta, Agustn Lpez-Mungua, and Clarita Olvera Structure/function relationship of multidomian levansucrase form Leuconostoc mesenteroides

COFFEE BREAK 11:00 AM 11:30 AM 11:30 AM SESSION V: BIOTIC AND ABIOTIC EFFECTS ON PLANT

FRUCTANS BIOSYNTHESIS Chair: Erika Mellado-Mojica

KEYNOTE Prof. David Livingston and Tan Tuong Using infra-red thermography to understand how fructan hydrolysis affects freezing patterns in rye

12:10 PM Carlos A. Salinas, Nicole Caamao, Markus Pauly, Paul Dupree, and Liliana Cardemil Structural modification of Aloe vera fructans induced by water stress

12:30 PM Patricia Dupr, Ivan Prez-Ramrez, Ernesto Tapia-Campos,

and Joaquin A. Qui-Zapata Preliminary results on the use of dahlia inulin to protect chili (Capsicum annuum) against Phytophthora blight

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LUNCH BREAK 1:00 - 3:00 PM CHEESE AND MEZCAL (Best Student Poster Award) 3:00 PM - 6:00 PM 3:00 PM SESSION VI: POSTERS EXHIBITION Chairs: Mercedes G. Lpez and Agustn Lpez-Mungua P-1 Binnaz Coskunkan, Deniz Turgut, Deniz Rende, Seyda Bucak,

Nihat Baysal, Rahmi Ozisk, and Ebru Toksoy Oner The structural diversity of sulfated and unmodified levan

polysaccharide chains studied with molecular dynamics simulations

P-2 Leticia Casas-Godoy, Javier Arrizon, Daniel Arrieta, and Georgina Sandoval Production of carbohydrate fatty acid esters by enzymatic acylation with Agave tequilana fructans

P-3 Adrian J. Enriquez-Valencia, Erika Mellado-Mojica, Mara L. Miranda-Ham, and R.M. Escobedo Gracia-Medrano Dynamic changes in soluble carbohydrate content during development of somatic embryo in Musa

P-4 Nadeshada Petkova Tr., Jorge Juan-Vicedo, Jos Luis Casas-Martnez, and Panteley P. Denev Determination of fructans in different agave species collected from Spain

P-5 Patricia A. Santiago-Garca, Cirenio Escamirosa, Erika Mellado-Mojica, and Mercedes G. Lpez Evaluation of fructans in Agave potatorum Zucc. during its development in the field

P-6 ukasz P. Tarkowski and Wim Van den Ende Fructan immunity in lettuce

P-7 Maxime Versluys, Lukasz P. Tarkowski, and Wim Van den Ende Sweet immunity in tabaco

P-8 Luz C. Vallejo-Garca, Mara E. Rodrguez-Alegra, and Agustn Lpez-Mungua Synthesis of diverse inulo-type fructooligosaccharides through bacterial enzymatic synthesis and hydrolysis from sucrose

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P-9 Keiji Ueno, Maiko Yoneta, Norio Shiomi, and Shuichi Onodera Characterization of fructan exohydrolase from Asparagus roots

P-10 Nilda Flores, Javier Arrizon, Marisela Gonzlez-Avila, Lorena Amaya, Sebastian Cavalitto, and Natalia L. Rojas Fructose syrup production from Agave tequilana fructans by enzymatic hydrolysis: Comparison between permeabilized cells of Kluyveromyces marxianus and a recombinant enzyme from Aspargillus kawachii

P-11 Ismael J. Romn Guzmn, Oscar A. Prez Ortiz, and Patricia Dupr In-silico analysis of a Dahlia variabilis sucrose:sucrose 1 fructosyltransferase (Dv 1-SST) like cDNA, promoter and transcripts accumulation in response to abiotic-related signals

P-12 Azucena Herrera-Gonzlez, Sandrine Morel, Magali Remaud-Simeon, Georgina Sandoval, Lorena Amaya, and Javier Arrizon Fructosyltransferase activity of non-Saccharomyces yeasts from mezcal fermentation for fructosylation of polyphenols

P-13 Jos M. Cruz Rubio and Juan F. Gmez Leyva Characterization of the fructan and the enzymes related to their biosynthesis in Agave salmiana

P-14 Agustn Albanesi P., Ivana Cavello, Lzaro Hernndez, Lorena Rojas, Sebastin Cavalitto, Marisela Gonzalez, and Javier Arrizn Screening of subantartic yeasts for -fructosidase activity

P-15 Arely V. Prez, June K. Simpson, and Jos J. Ordaz Fructans as dynamic molecules in Agave tequilana

P-16 Alejandro A. Espino, Hilda E. Dimas Estrada, David Ramrez Alvarado, Jos M. Cruz Rubio, Paola A. Palmeros Surez, and Juan F. Gmez Leyva Induction in biosynthesis of fructan in mutants plantlets of Agave tequilana induced with gamma rays Co60

P-17 Rosa M. Camacho Ruz Short chain agave fructans as carbon source for fungal fructan hydrolases production

P-18 Rosa M. Camacho Ruz and Jos de Jess Hernndez Lpez Agave fructans, from ancestral uses to industrialization

P-19 Jonatan de Jess Prez-Hernndez, Federico Fernndez-Islas, and Sandra Hernndez-Lpez Aislamiento y caracterizacin parcial de cepas bacterianas probiticas con potencial prebitico sobre inulina de agave

P-20 Elena Franco-Robles and Mercedes G. Lpez Agave fructans increase BDNF and GDNF and decrease oxidative stress in brain of high-fat diet-induced obese mice

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P-21 Mara I. Garca-Vieyra and Mercedes G. Lpez Preventive effect of agave fructans on bone loss and calcium absorption

P-22 Patricia A. Santiago-Garca and Mercedes G. Lpez Agavins from Agave angustifolia and Agave potatorum affect satiety-related hormones (GLP-1 and ghrelin) in mice

P-23 Yanelys Garca, Ramn Bocourt, Nereyda Albelo, Odalis Nuez, Brbara Rodrguez, Lzara Ayala, and Mercedes G. Lpez Agave fourcroydes fructans are beneficial to health

P-24 Emmanuel Ulbarri Navarro, Marcela De la Mora-Amutio, Lorena Moreno-Vilet, Mirna Estarrn-Espinosa, Julia Del Carmen Martnez-Rodrguez, and Javier Arrizn Evaluation of different sources of fructans on growth and SCFAs production in strains Bifidobacterium and Lactobacillus

P-25 Carmen Miramontes, Marcos A. Escalante, Ezequiel Delgado, Rosa I. Corona, F. Carbajal, J.F.A. Soltero, and Guillermo Toriz Encapsulation of ibuprofen into self-assembled A. tequilana fructan derivatives

P-26 Miriam Rodrguez-Aparicio, Karen Martnez-Cruz, Stefi Castillejos, Patricia Santiago-Garca, and Mercedes G. Lpez Spray-dried of blueberry extract using agave fructans as an encapsulating agent

P-27 Binnaz Coskunkan, Tunc Morova, Deniz Rende, Seyda Bucak, Nihat Baysal, Rahmi Ozisik, and Ebru Toskoy Oner In silico analysis of anticoagulant activity of levan sulfate via molecular docking simulations

P-28 Pamela I. Aldrete-Herrera, Luis Medina-Torres, Marisela Gonzlez-vila, Juan A. Ragazzo-Snchez, Montserrat Caldern-Santoyo, Mercedes G. Lpez, and Rosa I. Ortiz- Basurto Comparison of the physicochemical composition and degree of apparent polymerization of 5 varieties of wild agaves

P-29 Patricia A. Santiago-Garca, Erika Mellado-Mojica, Frank M. Len-Martnez, and Mercedes G. Lpez Evaluation of Agave angustifolia fructans as fat replacements in the cookies manufacture

P-30 Ana S. Garca-Gonzlez and Mercedes G. Lpez Unlocking the agave syrups mystery

P-31 Mara P. Quezada, Carlos A. Salinas, Martn M. Gotteland, and Liliana Cardemil Fructans from Aloe vera: A novel source for prebiotic compounds

P-32 M.R. Casas-Gonzlez, F.J. Rodrguez-Gonzlez, J.C. Contreras-Esquivel, and O.D. de la Rosa-Flores

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Study of prebiotic activity of agave syrup-plasticized thermoplastic starch (TPSA) films

P-33 Mercedes G. Lpez and Noureddine Benkeblia Profiling of short chain fructooligosaccharides (scFOS) of ackee (Blighia sapida Kenig) through different maturation stages

P-34 Souha Tohme, Zehra Semra Can, and Ebru Toksoy Oner Dual production of PHA and levan from waste resources by Halomonas cultures

P-35 Merve Erginer, Mehmet Erolu, Mehmet Airbali, and Ebru Toksoy ner Levan sulfate as a heparin-mimetic bioactive fructan

P-36 Masaru Iizuka Enzymatic synthesis of levan and oligosaccharides by use of levansucrase obtained from Bacillus natto -mechanism and some properties of the product obtained

P-37 Masaru Iizuka Introduction of commercial products available in Japan

THURSDAY, JUNE 30, 2016 9:00 AM PLENARY Chair: Mercedes G. Lpez

Prof. Gary S. Frost From fermentable carbohydrates to short chain fatty acids to appetite regulation

10:00 AM SESSION VII: FRUCTANS: PREBIOTICS, MICROBIOTA, AND

NUTRITION Chair: Mara A. Machado de Carvalho

Alicia Huazano-Garca and Mercedes G. Lpez

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Agavins modulate the cecum microbiota and reverse the metabolic disorders in overweight mice

10:20 AM Monika Mueller, Helmut Viernstein, Renate Lppert, and Werner Praznik Influence of the degree of polymerization and structure of fructans on their putative prebiotic effect

10:40 AM Gabriel Lpez-Velzquez, Chiharu Murata, Itzhel Garca-Torres, Ignacio de la Mora-de la Mora, Sergio Enrquez-Flores, Jos M. Cruz-Rubio, and Pedro Gutirrez-Castrelln Effects of fructans from Mexican agave in newborns fed with infant formula

COFFEE BREAK 11:00 AM 11:30 AM 11:30 AM Mara R. Romero-Lpez, Rosalva Mora-Escobedo, and Areli

Flores-Martnez Evaluation of anti-inflammatory effect of aguamiel (Agave atrovirens) in gastric ulcer model in rats

11:50 AM SESSION VIII: FRUCTANS BIOTECHNOLOGY

Chair: Noureddine Benkeblia Glben Avar, Ouzhan Gndz, and Ebru Toksoy ner Sulfated levan nanofibers for biomedical applications

12:10 PM SESSION IX: AGAVE FRUCTAN INDUSTRY: PAST, PRESENT, AND FUTURE Chair: Wim Van den Ende Anne Gschaedler The agaves: importance source of fructans used in various Mexican industries

12:50 PM Jos M. Cruz Rubio and Juan F. Gmez Leyva The state of the agave syrup and agave fructan industry in Mexico

LUNCH BREAK 1:10 - 3:00 PM

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3:00 PM SESSION X: AGAVE INDUSTRY Chair: Lzaro Hernndez Carlos Enrique Guilln Barrios CANITEC

3:20 PM Jos Gmez PREVENTY

3:40 PM Ana Isabel Chvez Lemus Campos Azules

4:00 PM Alvaro Cetto

IIDEA

4:20 PM Adolfo Prez Agilent Technologies Mexico

CLOSING REMARKS 7:00 PM INTERNATIONAL FRUCTAN SYMPOSIUM COMMITTEE

MEETING

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SPONSORS