8/18/2019 Question 10  Section c

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Question 10 (Compulsory)

Study the following statement:

Kaji pernyataan berikut:

You are given a sterile nutrient broth and Bacillus subtilis culture (bacteria culture)

''Anda diberikan bubur nutrient steril dan kultur Bacillus subtilis (kultur bakteria).

(a) Suggest one hypothesisto investigate the above statement ! " mark#

  Cadangkan satu hipotesis untuk menyiasat pernyataan di atas [1 markah]

 $otes: You can use

"the %&he higher(') the lower (*)

++viseversa% statement ,* 

-

.

You can just /,0Y the statement in the bo1

2

as your answer Both are accepted3

Answer:

"The higher the light intensity, the lower the growth of  Bacillus subtilis"

*emember4 use the named organism given in the 5uestion

(b) 6escribe one e1periment to test your hypothesis in l7(a) based on the following

criteria:  Huraikan satu eksperimen untuk mengkai hipotesis di 1!(a) berdasarkan kriteria

berikut"

(i) 8im of the e1periment !" mark#

  #uuan eksperimen [1 markah] 

(ii) 9dentification of variables !- marks#

 $engenalpasti pembolehubah [% markah]

(iii) ist of apparatus and materials !" mark#

  &enarai radas dan bahan [1 mark]

8/18/2019 Question 10  Section c

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(iv) 0rocedure or method !2 marks#

   rosedur atau kaedah [ markah]

(v) &abulation of data !" mark#

   enadualan data [1 mark]

8nswer:

(i) 8im: %&o study the effect of light intensity on the growth of Bacillussubtilis (bacteria)%

Note: &he answer must begin with "To study....." then followed by !and "on

the" !9n short the answer should look like this "To study........!...... on the..........!.........."

  ;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;;

(ii) ariables:; (the C#$CT way to write the answer)  'anipulated ; ight intensity

  *esponding ; &he growth of Bacillus subtilis

  /onstant ; (&emperature humidity nutrient p< of nutrient broth) ie other factorsthat can affect the growth of microorganisms or volume of nutrient broth+bacterial

culture or number of days

Notes: 0=8S= write down every variable separately3 6on>t just list all variables in one

 blow

?or e1ample (%#N& way to answer)  !'ri'les: ight intensity, growth of mi*roorg'nism 'nd +olume of nutrient

roth

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(iii) 8pparatus and materials: (this time 0=8S= write all apparatus @

materials together ; to avoid mistakes)

  '*teri'l *ulture, sterile nutrient roth, test tues, sterile *otton wool, syringe

 $ote: list all materials @ apparatus that are used in the e1periment

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(iv) 0rocedures: (you can answer in point form)

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• Set up apparatus as

shown in the diagram

above (this type of  

answer mustaccompanied with a

labelled diagram as

above)

• &ransfer 1 ml (/) of 

 bacterial culture in

each test tube and plug

them with sterile cotton wool

• 0lace the test tube in the following places:

•   8 ; under bright light A

•   B ; on the cabinet A '

•   / ; in a dark cupboard A

• Keep for - d'ys (/)

• ,bserve the *loudiness of the nutrient roth (*) in each test tube and record in the

table

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(v) 6ata: (in table form ; should clearly stated the ' and * on the title)

  (!)  (!)