real world surveillance, llc

Microbial Management to Improve OBW Discharge, H2O Injection and H2O Reuse

Real World Surveillance, LLC22nd Annual Produced Water Society Seminar

January 17, 18, 19th, 2012

Real World Surveillance, LLC Telephone: 601.250.6256

Johnny D. Richard, Owner/Operator Cell: 601.551.0395

Certified Minority/Veteran Business Enterprise Email: [email protected]

Website: www.realworldeye.com Email: [email protected]

“A Hands On, Eyes Open Company”

“A Real Eye in the Real World”

mailto:[email protected]

http://www.realworldeye.com/

mailto:[email protected]

REAL WORLD SURVEILLANCE LLC, ORGANIZATIONAL CHART

ME – Johnny D. Richard

(Whole Package)

I

(Formal Report & Training)

MYSELF

(Data & Summary)

LuminUltraMicrobiological

Monitoring –

2nd Gen. ATP Testing & Culture Back-up

Technical Surveillance –Onsite or Project

Management

Improve NPDES Performance, Microbial Mgmt, Solids Mgmt Programs, Ops Surveillance Skills, etc

“Microbial Management”

It’s a battle of the seen and the unseen.

Microbial contamination affects:• People (diseases)

• Wells, Vessels, Piping and Pipelines (Plugging, Souring, MIC)

• Higher Total Suspended Solids in the Produced H2O & Oil


Sessile -- Biomass Planktonic -- Floaters

“Microbial Management”If You Don’t Measure It, You Can’t Control It!

Mechanical Microbial Control Methods:

❑ UV (Ultraviolent) Light – DNA Structure Damage

❑ Magnets – Ionization of bacteria environment

Chemical Treatment: Biocides

❑ Microbiocides - chemicals that kill microorganisms,

❑ Biostats - chemicals that limit bacterial growth and/or activity,

❑ Chemical solvents used to clean up and dissolve solids.

Mechanical Bacteria TreatmentHalliburton’s “CleanStream® Service - Ultraviolet Light Bacteria Control

Process for Fracturing Fluid”

The cellular DNA of microorganisms like bacteria absorbs the energy from

the UV light, causing damage to their DNA structure this damage interferes

with many cellular processes including protein synthesis then the replication

of the chromosome prior to reproduction is impaired, leaving the bacteria

unable to produce proteins or replicate.

Mechanical Bacteria Treatment

The Eco1st Separation Enhancer "separates oil & water" by causing the water molecules to lose their

ability to bond with one another and any foreign contaminates contained therein. The Separation Enhancer

does this by breaking dipole bonds and stripping electrons from the water and the foreign contaminates

which ionizes the water into a positively charged state. When ionized the molecules repel one another and

make it easy for separation of these materials to occur.

Reduce need for chemicals and biocides. *Information pulled directly from ECO1ST website.

Microbial Test Methods

Traditional Test Methods:❖ Culture Test: Primarily Serial Dilution or BART Testers

Serial Dilution & Biological Activity Reaction Test (BART):

➢ Sulfate Reducing Bacteria - SRB,

➢ Acid Producing Bacteria – APB

➢ Heterotrophic Aerobic Bacteria – HAB

➢ Denitrifying Bacteria – DN

➢ Nitrifying Bacteria – N

➢ Iron Related Bacteria – IRB

❖ Rapid Bacterial Detection and Counting Methods➢ Microscopic Examination

➢ ATP Photometry

❖ Physical and/or fluid process changes: ➢ Biomass Coupons

➢ Inspection Spools, etc.

Method2nd Generation

ATPMicroscopic Examination

Culture TestsParticulate Analysis

BioassaysChemical Methods

What is detected?Total

Microorganisms

Total or Specific

Microorganisms

Culturable

Microorganisms

Suspended

Solids

Toxicity to

Surrogate

Organisms

Chemical

Constituents

Interferences in

detecting total living

biomass

None

Dead biomass;

non-biological

particles

UNABLE TO

MEASURE

Dead biomass;

non-biological

particles

UNABLE TO

MEASURE

UNABLE TO

MEASURE

How long to get

results?Minutes Minutes to Hours Days to Weeks Minutes to Hours

Minutes to

DaysMinutes

Can give results on-

site?Yes

Yes

(but difficult)No Yes No Yes

What types of

samples can be

tested?

Fluids & Solids Fluids & SolidsFluids, Re-

suspended SolidsFluids only

Fluids, Re-

suspended

Solids

Fluids & Solids

How much skill is

required?Low High Moderate Low High Low

What is the capital

cost?Moderate High Low Moderate

Moderate to

HighLow to Moderate

What is the cost per

test?Moderate Low Moderate Low to Moderate High Low to Moderate

What is its best use?

Total

microbiological

concentration

Population

diversity

Specific

microbiological

concentration

Total particlesEnvironmental

toxicity

Chemical

constituents

Microbial Test Method Comparison Table


• ATP stands for Adenosine

Triphosphate.

• ATP is the primary energy carrier

for all life (measures all cells).

• 1st Generation ATP test have

existed for 30+ years.

• LuminUltra has perfected the ability to measure ATP in water,

Wastewater, and organics via superior chemistry and methods.

• These 2nd Generation test kits are the only products

on the market that function in all energy sector applications

“Microbial Management”Project Description

– Improve & Minimize Biocide Treatment Effect on Produced H2O

– Compare Rapid Biological Test Method to Traditional Methods

Testing Protocol Baseline:– Baseline Data:

• Days since last biocide treatment

• Current treatment rates: Water sample to chemical account rep. for residuals

• BS&W (Ops Shake-outs) of affected vessels

• O&G of affected vessels: Field Batch Monitor & Non-protocol GR/GRS

• Millipore survey (10u Teflon) or Norman Filter (if not available onsite collect 1 qt, refrigerate and send in with non-protocol samples) *Your call

• ATP Baseline Survey (Oil, Water, Emulsion Pad & Solids Interface of Vessels)

• Serial Dilution - SRB/APB & BART SRB/APB Testers

• pH, Temperature & Visual analysis of oil and water phase

• Evaluate recycle streams into vessels when pumping

• Photos

*Note: Any chemical suspected of having an effect on the microbial levels of the produced water should be adjusted or optimize prior to start. Measure Wemco 02 levels


Testing Protocol Baseline cont’dOptional Baseline Data:– Chemical residue water sample– Temperature, pressure, pH, dissolved O2, dissolved H2S, etc– IC/ICP water analysis– Temperature profile of vessel for solids accumulation– Chemical residuals

Disposal Well (if applicable)– Disposal rate/pressure

Ensure all equipment are in good working order: Chemical injection system, O&G batch/online monitor, good supply of sample bottles, S.O. machine, etc.

Primary Contamination Sources: Formation, Vessels(Bottoms/Interface/Fluid Outlets), Wet/Bad Oil Tanks,

Treaters, & Production Sumps

Testing Locations

“Microbial Management”• Test results will be available the same day using the Lumin Ultra 2nd generation test

method in all fluids/solid samples collected. BARTs are maximum 10 days, whereas serial dilution are 7 – 28 days.

• Labeling, documentation and photos are key to the project and comparison with the serial dilution or BART’s if you decide to use them.

• It is recommended to clean the production sump if possible or treat with biocide at least twice before starting the project.

For heavily contaminated systems, e.g. high ATP values >100,000 pg/mL in all locations, you might want to see how effective is your biocide batch method first, after one week or one day. In such cases a continuous treatment maybe in order to establish some control. Continuous treatment rate can be controlled using the ATP method.

On the other hand, if there are minimum oil and water quality issues on your platform, you may have bacteria species that are not affecting the process.

Auxiliary WHRU/Steam systems that do not contain ethylene glycol should be checked also due the reality, if you lose your heat medium system, oil and water separation becomes more of a challenge. The same can be said for the drinking water and/or potable water system. Sick personnel means not much gets done.

“Microbial Management”Testing Protocol Biocide Treatment:

– Use the recommended treatment dosage and rate at the designated location.

– Estimate the time for the biocide to travel through system to the final discharge and/or re-injection point.

– Collect a visual sample at the first notice of change to the water outlet or one hour after treatment.

– Testing frequency is 4, 8, 16 & 24 hours on the first day of treatment and daily until ATP values stabilize at a low point and begin to climb back up again.

– It is a judgment call when to collect the serial dilutions, BART’s and other comparable methods. As a minimum to this first project, I recommend daily.

– Biocides sometimes damage bacteria cells and the ATP within the cells are not fully released. This is overcomed by adding the “LumiSolve Wash” after steps 1-3 in the QGA protocol. LumiSolve is a buffer that is used to re-dissolve extra-cellular ATP that may have been captured on the filter from damaged cells. This has been an issue when using Gluteraldehyde. A similar test can also be done with the LumiClean product.


Testing Protocol Biocide Treatment cont’d:

• Time biocide treatment

• Date/Time of sample, location, type of sample (water, emulsion or solids)

• Current treatment rates and send a water sample to the chemical account rep. for residuals of C.I., S.I., and Biocide

• BS&W (Ops Shake-outs) of affected vessels


• Millipore survey (10u Teflon) or Norman Filter (if not available onsite collect 1 qt, refrigerate and send in with non-protocol samples) *Your call

• ATP Survey (Oil, Water, Emulsion Pad & Solids Interface of Vessels)

• Serial Dilution - SRB/APB & BART SRB/APB Testers

• pH, Temperature & Visual analysis of oil and water phase

• Check status of recycle streams into vessels when testing

• Photos, notes or observations

Note: GR = EPA 1664 HEM, GRS = EPA SGT-HEM

“Microbial Management”Modified Testing Protocol: Baseline and Protocol Testing

• Date/Time of sample, location, type of sample (Before and After Biocide Treatment)

• Current treatment rates

• BS&W (Ops Shake-outs) of affected vessels: In lieu of S.O.’s, collect a 1000 mL sample and allow it to stand until fluids separate. Photo immediately after taking sample and after complete fluid separation.


• Millipore data : collect 1 qt sample, refrigerate and send in with non-protocol samples) *Your call

• ATP Survey: Collect samples in sterile vials (e.Coli samplers), allow sample to over flow, refrigerate and send in to specified lab for analyses.

• Serial Dilution - SRB/APB & BART SRB/APB Testers : Collect and send in to lab.

Modified Biocide Testing: 1) Test H2O outlet of vessel prior to biocide treatment, 2) Test H2O after treatment: Frequency: Day 1 (Hour 1, 4, 8, 16 & 24), then daily……


≠ ≠

Viable sub-populations

of total populations

Total bacterial

population

Estimate of activity

of total population

Verdict: All methods are unique!

What is Your Microbial Management Plan to Improve

OBW Discharge, H2O Injection and H2O Reuse?

Incorporate the2nd Generation ATP Technology

as the

“The First Line of Defense Tool”

Real World Surveillance, LLCMicrobial Management Programs

THANKS

QUESTIONS?

“Microbial Management”QGO-M: Quench-Gone Organic Modified

LuminUltra Quality Control

ApplicationGood Control

(pg cATP/mL)

Preventative Action

(pg cATP/mL)

Corrective Action

(pg cATP/mL)High-Purity Water < 0.1 0.1 to 1.0 > 1.0

Water for Consumption

(Potable, Sanitary) < 0.5 0.5 to 10 > 10

Raw Make-up Water

(Fresh, Brackish, Salt, Reclaimed) < 10 10 to 100 >100

Treated Process Water

(Cooling, Bottom Water, Oilfield)

Oxidizing Biocides*< 10 10 to 100 >100

Treated Process Water

(Cooling, Bottom Water, Oilfield)

Non-Oxidizing Biocides or Non-

Chemical Treatment **<100 100 to 1000 >1000

ATP Data from HED Chemical Free Debug Project

Sample

Point

UltraCheck

RLU

Date

Collected

Time

Collected

Date

Analyzed

Time

Analyzed ATP

RLU

Volume

FilteredcATP

dd-mmm-yyhh:mm

AM/PMdd-mmm-yy

hh:mm

AM/PMmL pg/mL

Raw Pond

Water 8024 21-Jun-11 9:45:00 AM 21-Jun-11

10:00:00

AM 306935 50 7650

Sample #1 8024 21-Jun-11 9:55:00 AM 21-Jun-11

10:05:00

AM 2104 50 52.44

Sample #2 8024 21-Jun-11 10:00:00 AM 21-Jun-11

10:30:00

AM 647 50 16.13

Raw H2O

Source #2 8024 21-Jun-11 11:00:00 AM 21-Jun-11

11:15:00

AM 78431 50 1955

Sample #3 8024 21-Jun-11 11:25:00 AM 21-Jun-11

11:40:00

AM 1476 50 36.79

Sample #4 8024 21-Jun-11 11:30:00 AM 21-Jun-11

11:55:00

AM 1016 50 25.32

HED: Pre-Frac H2O Treatment Test

Sample Point

Ultra

Check

RLU

Date Collected Date AnalyzedATP Volume

FilteredcATP Cell Count

RLU

dd-mmm-yy dd-mmm-yy mL pg/mL ME/mL

I Raw Water 10573 20-Oct-11 20-Oct-11 29493 50 557.9 557900

II HED Treated 10573 20-Oct-11 20-Oct-11 125 50 2.365 2365

III HED Treated + 2 Drops Clorox 10573 20-Oct-11 20-Oct-11 119 50 2.251 2251

IV HED Treated + 4 Drops Clorox 10573 20-Oct-11 20-Oct-11 91 50 1.721 1721

V HED Treated + 6 Drops Clorox 10573 20-Oct-11 20-Oct-11 119 50 2.251 2251

10/20/11 Reruns 10/24/11 50





10/20/11 Reruns After 1 Week





V HED Treated + 6 Shaken Re-Rerun 9948 20-Oct-11 28-Oct-11 79 50 1.588 1588

LuminUltra Technologies – April 2011 Newsletter

ASTM Approves Method for ATP Measurement in Fuels• On April 1st, 2011, ASTM International approved the new method D7687 (Standard Test

Method for Measurement of Cellular Adenosine Triphosphate in Fuel, Fuel/Water Mixtures and Fuel-Associated Water with Sample Concentration by Filtration). LuminUltra is pleased to announce that its QGO-M test kit is compliant with this new method. This new standard provides industry stakeholders with a rapid, portable and robust method for the quantification of total active biomass in a wide range of sample types that can be encountered in the liquid fuel handling sector.

The QGO-M test kit is also compliant with ASTM Standard E2694 (Standard Test Method for Measurement of Adenosine Triphosphate in Water-Miscible Metalworking Fluids). QGO-M is fast becoming an industry standard for the measurement of total biomass via ATP in a myriad of organic-based sample types, ranging from metalworking fluids to fuels to organic chemicals and even water samples laden with organic contaminants. Click here to learn more about the QGO-M test kit and the applications to which it applies!

http://cts.vresp.com/c/?LuminUltraTechnologi/1fbfd995be/30cf69af0d/a4cf7f81dd

http://cts.vresp.com/c/?LuminUltraTechnologi/1fbfd995be/30cf69af0d/fcf0375469/[email protected]&utm_source=VerticalResponse&utm_medium=Email&utm_term=here&utm_campaign=April%202011%20Newsletter

BART’s – Biological Reaction Tester

Principal Microbial Groups Determinable Using the BART™ Biodetectors

Microbial Community Selective Culture Medium*

Iron Related Bacteria IRB Winogradsky’s medium

Sulfate Reducing Bacteria RB Postgate’s medium

Slime Forming Bacteria SLYM Glucose Peptone medium

Heterotrophic Aerobic Bacteria HAB Sugar Peptone medium

Algae ALGE Bold’s medium

Fluorescent Pseudomonads FLOR Peptone base medium

Denitrifying Bacteria DN Nitrate Peptone medium

Nitrifying Bacteria N Ammonium salts medium

Acid Producing Bacteria APB Modified Glucose Peptone medium

Biochemical Oxygen Demand BOD Modified Sugar Peptone medium

BART Tester

BART Tester Interpretation Guide

BART Result Interpretation

BART Bacteria Population Estimate

BART Results Photos

BART Results Photos cont’d

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