research report 2011 - gluck.ca.uky.edu

Research Report 2011

University of KentuckyMaxwell H. Gluck Equine Research Center

Improving the Health and Well-being of the Horse

Research Report 2011Improving the Health and Well-being of the Horse

University of KentuckyMaxwell H. Gluck Equine Research Center

Department of Veterinary Science108 Gluck Equine Research Center

Lexington, KY 40546-0099

Phone: (859) 257-4757Fax: (859) 257-8542

Website: http://www.ca.uky.edu/gluck

The University of Kentucky is committed to a policy of providing employment opportunities to all qualified people regardless of economic or social status and will not discriminate on the basis of race, color, ethnic origin, national origin, creed, religion, political belief, sex, sexual orientation, marital status, age, veteran status, or physical or mental disability.

Published in 2012 by the Gluck Equine Research Foundation, University of Kentucky

Dean, College of Agriculture: M. Scott Smith, PhD

Associate Dean for Research and Director, Kentucky Agricultural Experiment Station: Nancy M. Cox, PhD

Gluck Equine Research Center Director and Chair of the Department of Veterinary Science: Mats H.T. Troedsson, DVM, PhD, Dipl. ACT

UK Ag Equine Director and Gluck Equine Research Foundation Executive Director: Edward L. Squires, MS, PhD, Hon. Dipl. ACT

Design and layout: Jenny BlandfordPrinted by: Thoroughbred Printing

Cover photo: Matt Barton

UK Gluck Equine Research Foundation 2011 Board of Directors

OFFICERS

Dr. Walter Zent, ChairMr. Tom Goncharoff, Vice Chair

Dr. Eli Capilouto, PresidentDr. Kumble R. Subbaswamy, Vice President

Dr. M. Scott Smith, SecretaryMrs. Angela S. Martin, Treasurer

MEMBERS

Mr. Bruce Addington Mrs. Jane Beshear Dr. Stuart Brown Mr. Case Clay Dr. Nancy Cox Dr. Rolf Embertson Mr. William S. Farish, Jr. Mrs. Ginny Grulke Mr. G. Watts Humphrey, Jr. Mrs. Lisa Lourie Dr. Jamie MacLeod

Dr. Stephen Reed Mr. Dan Rosenberg Dr. Robert Stout Mr. David Switzer Mr. Oliver Tait Dr. Mats Troedsson Mr. F.E. “Butch” Wise Dr. Naoya Yoshida Mr. Chris Young Mr. Art Zubrod

A Letter from the Director 5

About the Gluck Equine Research Center 6-7

Research Programs and Accomplishments Genetics and Genomics 10-11 Immunology 12-13 Infectious Diseases 14-19

Musculoskeletal Sciences 20Parasitology 21-22Pharmacology/Toxicology 23Reproductive Health 24-26

Gluck Equine Research Center Grants 28-29

Scientific PublicationsBooks/Chapters in Books 31-32 Refereed Journal Articles 32-36

Non-Refereed Journal Articles 36-37 Seminars and Papers Presented 38-41

Gluck Equine Research Center Donors 43

Table of Contents

Research Report 2011 • 5

Thanks to a generous donation by the Gluck family, the Maxwell H. Gluck Equine Research Center opened its doors to a new laboratory and office building to accommodate researchers from the Department of Veterinary Science 25 years ago. During the past 25 years, the Gluck Center has developed into a unique equine research unit within the University of Kentucky College of Agriculture, with internationally-recognized faculty members, research staff and graduate students devoting all their time to research that benefits the health and well-being of horses around the world.

Great accomplishments have been made by the research groups in the areas of genetics and genomics, infectious diseases and immunology, musculoskeletal sciences, parasitology, pharmacology/toxicology and reproductive health. Vac-cines and diagnostic tests that are used to prevent and diagnose diseases have been developed, contributions to the sequencing of the equine genome has been made, reference standards have been established for detection of performance enhancing drugs, and causes of costly pregnancy losses have been identified and effective management and treatments have been developed.

Today we are facing continuous and new challenges in equine health and well-being with the threats of new emerging infectious diseases and bacterial resis-tance to antibiotics. Other issues are parasite resistance to dewormers, stricter regulations on medication for race and performance horses, an anticipated flow of genetic information through the sequencing of the equine genome, debilitat-ing musculoskeletal injuries in the equine athlete, rapidly developing research on regenerative medicine and imminent threats to equine breeding industries by reproductive diseases and abortions. The Gluck Center is well prepared for these challenges. As the only scientific institution in the United States that has faculty working full-time on equine health issues, the Gluck Center will continue to play an important role in the horse and veterinary communities. We are looking forward to the next 25 years at the Gluck Center with confidence.

As I have stated so many times before, we could not have accomplished what has been done at the Gluck Center over the past 25 years if we did not have a devoted support from individuals and organizations within the equine commu-nity. Your financial support and contributions has been the engine that drives our research and that eventually benefits the health and well-being of our horses. Your support is more important than ever in these challenging financial times.

Thank you for your past and future contributions to our program!

Dr. Mats Troedsson, DVM, PhD, Dipl. ACTGluck Equine Research Center Director

and Department of Veterinary Science [email protected]

Dr. Mats Troedsson

A LETTER FROM THE DIRECTORThe Maxwell H. Gluck Equine Research Center celebrates

its 25th anniversary in 2012...


HistoryThe Maxwell H. Gluck Equine Research Center is the

only scientific institute in the United States with nearly all faculty conducting full-time research in equine health and diseases.

Construction began on the 81,000 square foot facility in 1986 and was completed in 1987. The center is named af-ter the late Maxwell H. Gluck, owner of Elmendorf Farm in Lexington. Maxwell Gluck and his wife, Muriel, gener-ously donated $3 million to the University of Kentucky in 1983 for construction of the research facility on the condi-tion the gift be matched by $3 million from the state and $3 million from members of the horse industry.

Research The mission of the Gluck Center is scientific discov-

ery, education and dissemination of knowledge for the benefit of the health and well-being of horses.

Today, the Gluck Center faculty conduct equine re-search in six targeted areas: genetics and genomics, infec-tious diseases and immunology, musculoskeletal science, parasitology, pharmacology/toxicology and reproductive health.

Major research accomplishments of researchers at the Gluck Center has had an international impact on equine research. Some of the major research accomplishments include:

• World Organisation for Animal Health (O.I.E.)-des-

ignated world reference laboratory for equine rhi-nopneumonitis, equine influenza and equine viral arteritis

• Developed six major vaccines to protect against strangles, equine influenza, equine rhinopneumoni-tis, equine viral arteritis, the shaker foal syndrome (toxoinfectious botulism) and validated field safety and efficacy of equine rotavirus vaccine

• Developed diagnostic serological tests for con-tagious equine metritis (CEM), Tyzzer’s disease, equine protozoal myeloencephalitis (EPM), equine herpesvirus myeloencephalopathy, strangles and equine viral arteritis

• Developed enzyme-linked immunosorbent assay (ELISA) test for drug detection

• Demonstrated the usefulness of artificial lights and progesterone/estradiol treatments for hastening the onset of the breeding season

• Determined the genetic basis for and developed tests for inheritance of certain color coat traits

• Provided leadership in the sequencing of the com-plete genome of the horse and structural character-ization of horse genes

• Performed the definitive experiments that identified the cause of Mare Reproductive Loss Syndrome

Equine Research Hall of Fame The Equine Research Hall of Fame, established by the

The Maxwell H. Gluck Equine Research Center on the University of Kentucky campus

ABOUT THE MAXWELL H. GLUCK EQUINE RESERCH CENTER


The Equine Research Hall of Fame provides a lasting tribute to the most renowned equine researchers in a vari-ety of disciplines and serves as an international forum for honoring outstanding achievements in equine research.

Gluck Equine Research FoundationThe Gluck Equine Research Foundation was formed as

a non-profit organization to provide the exchange of in-formation between the Gluck Center and the horse indus-try and to secure research funds.

Since the Foundation’s inception, it has been highly supportive in raising funds for equine research, endowed faculty positions, and facilities. Funding for graduate stu-dent support has allowed Gluck Center faculty to educate the next generation of scientists.

Industry OutreachProviding research information to the equine commu-

nity is an important facet of the Gluck Center. Informa-tion is shared through the Gluck Center’s website, www.ca.uky.edu/gluck, and three newsletters:

• Bluegrass Equine Digest (monthly)• Lloyd’s Equine Disease Quarterly• Research & Service Report (biennially)

Department of Veterinary ScienceThe Gluck Center is part of the Department of Veteri-

nary Science along with the Animal Genetics Testing and Research Laboratory (AGTRL) and the Veterinary Diag-nostic Laboratory (VDL).

The mission of the Department of Veterinary Science is to assure the health and viability of animal agriculture through teaching, discovery, research and service.

Faculty in the Department of Veterinary Science fre-quently collaborate on research projects with faculty in UK’s College of Agriculture and College of Medicine, with veterinarians in central Kentucky and scientists at other institutions.

The Gluck Center is also part of the UK Ag Equine Programs (formerly the UK Equine Initiative), an over-arching concept for all equine activities in the College of Agriculture. Created in 2005 as a front door to equine programs at UK, the UK Ag Equine Program’s mission is to discover, share and apply new knowledge that will en-hance the health, performance and management of horses commensurate with the signature status of Kentucky’s equine industry.

University of Kentucky Equine Research Foundation (now the UK Gluck Equine Research Foundation), hon-ors those distinguished researchers who have dedicated their careers to equine science. The Hall of Fame is at the Gluck Center.

On Dec. 1, 1990, 12 scientists became the first induct-ees into this prestigious hall. Inductees are selected for the honor by an international scientific committee that evaluates the achievements and contributions of eminent researchers who were nominated by their peers and col-leagues. The inductees, active, retired or deceased, are in-dividuals from throughout the world who have expanded the body of knowledge of equine science through their contributions to basic or applied research.

ABOUT THE MAXWELL H. GLUCK EQUINE RESERCH CENTER

The late Maxwell and Muriel Gluck (top) do-nated $3 million to the University of Kentucky for construction of the research facility on the condition the gift be matched by the state and members of the horse industry. (Below) James Bassett III, former president of Keeneland, Mu-riel Gluck and former UK President Otis A. Singletary broke ground for the center in 1985.

Twenty-three faculty at the Gluck Equine Research Center are assisted by students,

post docs, research staff and visiting scientists in conducting research in the areas of:

Genetics and Genomics

Immunology

Infectious Diseases

Musculoskeletal Sciences

Parasitology

Pharmacology/Toxicology

Reproductive Health

Some of the world’s top scientists are drawn to the Gluck Center to provide solutions to equine health

problems. Gluck Center faculty also respond to some of the equine industries toughest problems.


GENETICS AND GENOMICSRESEARCH SNAPSHOTCoat color genetics Contracted foal syndromeCytogenetics and infertilityDwarfism Genetics and equine arteritis virusJunctional epidermolysis bullosa (JEB)Parrot mouth

FACULTYErnie Bailey, ProfessorKathryn Graves, Assistant Clinical ProfessorTeri Lear, Associate ProfessorJames MacLeod, Professor (See page 20)

Education: PhD – University of California-Davis (Genetics), 1980MS – University of California-Davis (Comparative Pathology), 1975BS – University of California-Davis (Genetics), 1973 Interest: Immunogenetics and genomics—We are interested in the genetic influences on the innate and adaptive immune systems which protect the horse from infectious diseases. Other interests include the development of the genetic map for horses and investigation of genes involved in the health of the horse such as contracted tendons, extreme lordosis and dwarfism. Projects: •Genomics and gene mapping in horses.•Investigation of the hereditary aspects of EIPH, swayback, dwarfism, cataracts and equine arteritis virus susceptibility.

Graduate student•John Eberth, MS Candidate – Dwarfism

Education: PhD – Cornell University, 1985BS – Cook College, Rutgers University, 1980 Interest: Overseeing a high quality Animal Genetics Testing and Research Laboratory and providing genotyping services to 50 equine registries. In addition, the lab offers specific tests for color genes and heritable disease mutations. Projects: •Develop new DNA-based color tests.•Candidate gene sequencing to identify causative mutations for heritable diseases.

Ernie Bailey, PhDProfessor

Kathryn Graves, PhDAssistant Clinical

ProfessorDirector, Animal

Genetics Testing andResearch Laboratory


Horse metaphase chromosomes (the blue sticks, nuclei are round) from a male horse. Green and red spots are horse genes mapping to the X chromo-some. Note: Males have one X and one Y chromosome, thus spots only show up on one X and not two as you would see in females with two X chromo-somes. (Photo: Teri Lear)

A “G gnome” stands in front of Twi-light, a Thoroughbred mare at Cornell University, who was the first horse ge-netically sequenced in 2007. (Photo: Ernie Bailey)

Education: PhD – University of Kentucky (Genetics), 1997MS – University of Louisville (Cytogenetics/Zoology), 1986BA – Indiana University Southeast (Zoology/Field Biology), 1975

Interest: Providing clinical cytogenetics services to the horse industry. Identifying genes causing inherited diseases such as contracted foal syndrome, lordosis in Saddlebreds, dwarfism, parrot mouth, etc. I also provide clinical cyto-genetic services to veterinarians in the United States and abroad. This has resulted in the detection of chromosomal abnormalities affecting health and fertility.

Clinical Cytogenetics Service: In keeping with the service and outreach mission of the University, my lab offers clinical cytogenetics services to equine veterinarians. Between 1.5% and 3% of the general horse population carries a chromosome abnormality including abnormalities of sex chromo-somes, trisomy and chromosome translocations. Chromosome abnormalities can profoundly affect fertility and survival. Cytogenetic techniques used in my lab can identify chromosome abnormalities causing infertility, ambiguous sex and congenital abnormalities. We have identified chromosome abnormal-ities affecting mare fertility, causing severe congenital abnormalities in foals and causing sexual ambiguity (Lear et al. 1999; Lear and Layton 2002; Lear et al. 2008; Lear and Bailey 2008; Villagomez et al. 2010; Lear and McGee 2011).

Projects: •Equine chromosome disorders causing repeated early embryonic loss and congenital abnormalities.•Identifying the cause of Contracted Foal Syndrome. •Causes of XY sex-reversal syndrome in horses.•Behavioral genomics of the White-Throated Sparrow.•Genomic gains and losses that cause congenital abnormalities in foals.

Graduate students and research staff:•Rose McGee, MS (graduated, 2011) – Disorders of sexual development in horses •Judy Lundquist, Research Technician•Allison Sparling, MS Candidate – Characterization of horse-specific genes

GENETICS AND GENOMICS

Teri Lear, PhDAssociate Professor

RESEARCH SNAPSHOTThe development of immunity in the foalThe immune response in aged horsesIdentification of vaccine-induced protective immune responsesCharacterization of inflammatory responses in the horseCytokine regulation of immunity in the horseLawsonia intracellularis enteropathyRhodococcal pneumoniaVaccine development

FACULTYAmanda Adams, Assistant ProfessorDavid Horohov, Professor

Education:Postdoctoral Scholar – University of Kentucky (Equine Immunology), 2011PhD – University of Kentucky (Veterinary Science), 2008 BS – Stephens College (Biology), 2003

Interest:My research interests are focused on characterizing the immune system of the geriatric horse, by identifying mechanisms responsible for immunosenescence, inflamm-aging and altered immune responses to vaccination. The goal is to improve the health and well-being of the aged horse, by identifying interventions that have the potential to improve the function and redox state of the immune system. In addition, I have established research funding to further understand the effect of adiposity on the inflammatory response of the horse, in particular the equine metabolic syndrome (EMS) horse. There is a growing need to understand mechanisms responsible and pathways involved in equine metabolic syndrome. Thus, my goal is to identify potential treatments that target both the inflammatory and metabolic component of EMS.

Projects:•Studies ongoing to determine what role nutritional intervention has on age-relat-ed changes in immune function, in particular chronic inflammation or inflamm-aging.•Studies ongoing to develop the horse as a model system for human aging to fur-ther understand age-related changes in the immune response, in particular mecha-nisms responsible for inflamm-aging, focusing on T-cell membrane rafts.•Identifying biomarkers of aging to better predict the “biological age” of the horse.•Developing novel diagnostic tools for diagnosis of pituitary pars intermedia dys-function (PPID) and evaluating novel treatments for PPID horses.•Studies ongoing to characterize mechanisms responsible for Equine Metabolic Sydrome (EMS) and to identify possible treatments for EMS.•To characterize what role gut microflora may play in inflamm-aging and EMS.

Amanda A. Adams, PhDAssistant Professor

IMMUNOLOGY


Education:Professor of Veterinary Immunology, Louisiana State University, 1988-2003Fellowship – FDA, Bethesda, MD, 1986-1988PhD – University of Tennessee, 1985 MS – Purdue University, 1981BS – Pennsylvania State University, 1978

Interest: My group continues to investigate the immune responses of horses to various infectious diseases. We are also especially interested in infections that occur in the foal, such as Rhodococcus equi and Lawsonia intracellularis. We also collaborate with other groups to study the characterization of cell-mediated immune responses in EIA-infected and vaccinated horses, vaccination of foals against equine influenza virus, further investiga-tions into parasite immunology and the characterization of inflammatory responses to exercise.

Projects: •Underlying immunological basis for the susceptibility of foals to infection with Rho-dococcus equi, a cause of pneumonia in foals less than 3 months old. •Investigating the underlying molecular mechanism for low levels of interferon-gamma, a cytokine that plays a central role in resistance to R. equi and other pathogenic organ-isms. (Linshuang Sun)•Infections that occur later in the life of the foal, such as Lawsonia intracellularis, the causative agent for equine proliferative enteropathy (EPE). (Allen Page and Amanda Adams)•Older horse immune function. Collaborating with Kristine Urshel, UK Department of Animal Science, who is interested in the effect of aging on protein metabolism in horses, and Nicholas Frank, University of Tennessee, who is interested in metabolic syndrome in horses. Frank’s group is also interested in obesity-related inflammatory changes and their role in the induction of metabolic disease in the horse. •Cell-mediated immune responses in EIA-infected and vaccinated horses. The goal will be to identify immunological responses that may be important in controlling viral repli-cation and disease. (Chong Liu, in collaboration with Charles Issel at the Gluck Center and Ron Montelaro at Pittsburgh) •Other collaboration projects include: vaccination of foals against equine influenza vi-rus with Thomas Chambers at the Gluck Center; parasite immunology with Gene Lyons at the Gluck Center; and characterization of inflammatory responses to exercise with Ken McKeever, at Rutgers University. Our goal is to identify immunological markers for exercise-induced inflammation in the horse and to determine if these correlate with the risk of injury in the performance horse.

Graduate students, research staff, undergraduates and visiting scientists:•Alex Betancourt, Research Technician•Zhangbin Gong, Visiting Scientist •Chong Liu, PhD Candidate – Cell mediated immunity to EIAV•Allen Page, PhD Candidate – Lawsonia intracellularis infection and immunity•Lingshuang Sun, PhD Candidate – Regulation of interferon in foals•Macarena Sanz, MS Candidate – Immunology of the young horse Undergraduates: Courtney Carroll, Amber Simpson and Craig StewartVisiting Students: Jordan Dunham, MS Candidate, Wales; Lisa Tadros, PhD Candidate, University of Tennessee; Luis Prieto, PhD Candidate, University of Tennessee; and Whitney Zoll, DVM Candidate, Michigan State University

David W. Horohov, PhD William Robert Mills Chair

& Professor

IMMUNOLOGY


RESEARCH SNAPSHOTEquine rhinopneumonitisEquine influenzaEquine viral arteritis Equine infectious anemiaEquine rotaviral enteritis Strangles and other equine streptococcal diseasesEquine leptospirosisEquine clostridial enteritisDiagnostic test developmentVaccine developmentDisease surveillance and reporting Biosecurity/Disaster preparedness

FACULTYSergey Artiushin, Assistant ProfessorUdeni Balasuriya, ProfessorThomas Chambers, Associate ProfessorFrank Cook, Associate ProfessorRoberta Dwyer, ProfessorCharles Issel, ProfessorJohn Timoney, ProfessorPeter Timoney, Professor

Education: PhD – Moscow State University (Microbiology), 1981MS – Moscow Veterinary Academy (Biophysics), 1973 Interest: Research interests are focused on molecular studies of Streptococcus equi, Streptococcus zooepidemicus, and Leptospira interrogans. S. equi causes strangles and S. zooepidemicus is responsible for endometritis in mares. Leptospirosis can cause abortion and stillbirth as well as recurrent uveitis (a major cause of equine blindness). Projects: •Development of rapid diagnostic assays for identification of bacterial pathogens.•Study of surface and secreted proteins of Streptococcus as virulence factors and potential protective antigens.•Analysis of genetic variations in Streptococcus.•Identification of virulence factors of S. zooepidemicus responsible for developing acute infection in dogs and horses.

Sergey Artiushin, PhDAssistant Professor

Education: PhD – University of California-Davis (Comparative Pathology with special emphasis in molecular virology), 1996MS – University of California-Davis (Comparative Pathology with special emphasis in diag-nostic pathology), 1991BVSc – Faculty of Veterinary Medicine and Animal Science, University of Peradeniya, Peradeniya, Sri Lanka, 1985

Interest: The major research focus of my laboratory is to characterize the molecular epidemiology and pathogenesis of equine arteritis virus (EAV) and equine herpesvirus-1 (EHV-1) infec-tions of horses and develop improved recombinant vaccines to prevent infection of horses with these viruses, as well as to develop improved tests to diagnose the infection. In addi-tion, I have established national and international collaborations to facilitate exchange of scientists, reagents and information focused on EAV, EHV-1 and other equine viral diseases. My laboratory also provides a dynamic, first-rate research training environment to develop the next generation of research scientists.

Udeni Balasuriya, PhD, MS, BVSc

Professor


INFECTIOUS DISEASES

Projects:•Molecular characterization of equine arteritis virus (EAV) and equine herpesvirus-1 (EHV-1).•Definitively characterize the molecular epidemiology and pathogenesis of EAV and EHV-1 infections of horses and develop improved recombinant vaccines to prevent infection of horses with these viruses as well as improved tests to diagnose the infection. •Molecular mechanisms of viral pathogenesis and characterization of host immune re-sponse to EAV infection. •Host-virus interactions (e.g. analysis of individual viral genes/gene products and their interaction with host).•Development of new diagnostic and vaccine technologies, define the epidemiology and pathogenesis of other important viral diseases of the horse, and the recognition of novel and emerging viral diseases of the horse.•Establish molecular diagnostic assays for rapid detection of foreign, emerging and zoo-notic diseases of horses. •Establish national and international collaborations to facilitate exchange of scientists, students, reagents and information, all focused on EAV, EHV-1 and other important viral diseases of the horse.

Services:•Provide assistance with molecular diagnostics (RT-PCR and real-time RT-PCR).•Testing of clinical specimens submitted to the OIE designated reference laboratory for equine viral arteritis (EVA) at the Gluck Center.•Testing of clinical specimens submitted to the OIE designated reference laboratory for equine influenza infections and for equine herpes virus at the Gluck Center.•Provide molecular diagnostic reagents to diagnostic laboratories.•Provide advice on equine viral arteritis to veterinarians and equine industry associates over the phone.

Graduate students and research staff:•Juliana Campos, MS Candidate – Genetic susceptability of stallions to EAV•Yun Young Go, PhD (graduated 2011) – Molecular and genomic approaches to under-standing EAV pathogenesis•Pamela Henney, Research Specialist •Yanqiu Li, Postdoctoral Scholar – Molecular characterization of neurovirulent EHV-1•Zhengchun Lu, PhD Candidate – EAV attachment and entry and molecular diagnostics•Kristin Pfahl, MS Candidate – Development and validation of improved serological as-says for EVA•Kathryn Smith, PhD Candidate – Virulence determinants of EHV-1


INFECTIOUS DISEASES

R. Frank Cook, PhDAssociate Professor

Education: PhD – University of Warwick (Virology), 1980BSc – University of Sussex (Biochemistry), 1976 Interest: My research focuses on Equine Infectious Anemia Virus (EIAV), a virus closely related to HIV in humans. My most recent interests are in the field of vaccine design. Projects: •Design of vaccines against all lentiviruses including HIV-1.•Provide purified antigens for inclusion in commercial USDA-approved test kits.•Strategies to enhance efficacy of DNA vaccination in the horse (i.e., the use of cytokines to enhance the efficacy of vaccines).•Molecular epidemiology of equine herpesviruses.•Genetic basis of differing susceptibility to disease and immune responses to vaccinations.

Graduate student:•Debbie Even, PhD (graduated, 2011) – Manipulating immune responses to DNA vaccines in the horse

Education: PhD – University of Notre Dame (Microbiology), 1982BS – University of Notre Dame (Pre-Professional Studies), 1975

Interest:Equine influenza is the leading cause of respiratory disease in Kentucky and the world. My major interest is to study the innate immune responses to the influenza virus and herpes virus. I am also interested in the development of vaccines for influenza and herpes virus. I am involved in infectious disease control and surveillance both nationally and internationally.

Projects: •Infectious diseases and immunology.•Testing in equines of a second-generation modified-live virus equine influenza vaccine.•Testing/validation of second-generation rapid diagnostic tests for equine influenza.•Testing in equines of novel DNA-based vaccines for equine influenza.•Testing in equines of new vaccination protocols for equine influenza using an existing commercial vaccine.•Collaborator on development of the most up-to-date phylogenic description of the strains of equine influenza virus in circulation since 1990.•Collaborator on development of a new real-time RT-PCR assay for equine influenza.•OIE International Reference Laboratory for equine influenza.

Graduate students and research staff: •Sanjay Sarkar, PhD Candidate – Innate immunity to equine viral respiratory diseases•Ashish Tiwari, PhD Candidate – Innate immunity to equine viral respiratory diseases•Stephanie Reedy, Research Specialist •Liang Zhang, PhD (graduated 2011) – Comparative pathogenesis of equine- and equine-2 influenza viruses

Thomas Chambers, PhDAssociate Professor


INFECTIOUS DISEASES

Roberta Dwyer, DVM, MS, DACVPM

Professor

Education: DVM – Iowa State University, 1985MS – University of Kentucky, 1990Diplomate, American College of Veterinary Preventive Medicine, 1993Board Certified in Epidemiology, ACVPM, 2003 Interest: Equine preventive medicine and infectious diseases, disease outbreak investigation and epidemiology, biosecurity, disaster preparedness and response, risk reduction to agroter-rorism and pre-veterinary advising and undergraduate teaching.

Projects: •Consultations for veterinarians, farm managers and horse owners.•Biosecurity plan development for veterinary and farm facilities.•AAEP on-call media veterinarian for infectious disease issues.•Instructor for a national extension program, “Strengthening Community Agrosecurity Plans.”•Co-editor of Lloyd’s Equine Disease Quarterly.•Planning section chief for a national Incident Management Type II team (disaster re-sponse team).

Charles Issel, DVM, PhDWright-Markey Chair in

Equine Infectious Diseases & Professor

Education: Diplomate – American College of Veterinary Microbiologists, 1976PhD – University of Wisconsin (Veterinary Science), 1973MS – University of Wisconsin (Veterinary Science), 1971 DVM – University of California–Davis, 1969AB – University of California–Berkeley (Zoology), 1965

Interest: Our research, continuous since 1974, involves equine infectious anemia from A to Z. We are working with the national and international veterinary community to develop and implement sensitive, specific and practical diagnostic tests for EIA to complement the “Coggins” test in effective control programs. Our work indicates that we should adopt a three-tiered testing approach which would more accurately detect positive horses with difficult to read AGID test reactions. At the same time we are studying the intricacies of the EIA virus (EIAV), a lentivirus, in an attempt to define the genetic and antigenic variations in this highly mutable agent and how it impacts protective immunity, i.e., vaccine design and efficacy. This work is valuable in its own right as well as being of comparative value as a model for AIDS. Our projects with the USDA have resulted in a DVD package that includes our video from 1996 and a new video from 2010, “Equine Infectious Anemia Testing – Refining our approach.” Both videos are available online at http://www.aphis.usda.gov/animal_health/animal_diseases/eia/. To obtain the DVD package, visit http://www.aphis.usda.gov/vs/nahss/equine/eia/index.htm.

Projects: •Improve diagnosis and control of EIA.•Develop effective vaccines against EIA.•Provide high quality reagents for use in testing for diseases.•For more information on EIA please see our website at: http://dept.ca.uky.edu/eia/.

Research staff:•Sheila J. Cook, Research Scientist


INFECTIOUS DISEASES

A scanning electron micrograph (left) shows adherence of Streptococcus equi, the cause of equine strangles, to stratified squamous epithelium of the equine lingual tonsil. The photo (right) shows S equi within the epithe-lium of the nasopharyngeal tonsil of a horse three hours after infection. (Photos: John Timoney)

Education: PhD – National University of Ireland, 1969MS – University of Wisconsin, 1967MVB, MRCVS – University College, Dublin, 1965BSc – University College, Dublin (Biology), 1961

Interest: Focus is on equine infectious disease caused by streptococci, leptospira, salmonella and clostridia. Ultimate goals include development of improved vaccines, diagnostics and de-sign of strategies effective in management of outbreaks and detection of infected horses.

Projects: •Use of the binding sites of Toxins A and B of Clostridium difficile as immunogens to gen-erate toxin neutralizing antibody in pregnant mares. •Identification and regulation of virulence factors of Streptococcus zooepidemicus from acute equine and canine pneumonias.•Wildlife source(s) of the specific genotype of Leptospira interrogans serovar Pomona responsible for equine abortions in Kentucky.•Development of novel modalities for intranasal vaccination of horses against strangles.•Rapid diagnostic assays for Streptococcus equi and Leptospira interrogans in equine clinical specimens. •Development of protocols for prepartum immunization of mares for prevention of neona-tal enterocolitis caused by clostridia and salmonella.•Role of bacteriophage in virulence and evolution of Streptococcus equi.•Identification of proteins of Leptospira interrogans induced following ocular and placen-tal infection of the horse.•Interaction of Streptococcus equi with the equine tonsillar complex.

Postdoctoral/Graduate students and research staff:•Rafaela De Negri, PhD Candidate – Comparative aspects of humoral and cell mediated responses of horses to infection by Streptococcus equi and zooepidemicus. Interaction of IdeE 2 of S. equi with equine platelets and its role in the pathogenesis of the acute phase of strangles.•Michael Fettinger, Research Technician•Sridhar Velineni, Postdoctoral Scholar – Protective immunogenicity of novel immuno-reactive proteins of Streptococcus zooepidemicus. Temperature regulated expression of capsule, hyaluronidase and associated virulence factors in clinical isolates of S. zooepi-demicus.

John Timoney, MVB, MRCVS, MS, PhD

Professor


INFECTIOUS DISEASES

Education: FRCVS – Royal College of Veterinary Surgeons, 1978PhD – University of Dublin, 1974MS – University of Illinois, 1966MVB (Hons) – National University of Ireland, 1964 Interest: Among the diseases of major interest are equine viral arteritis (EVA), contagious equine metritis (CEM) and equine rhinopneumonitis. Also, development of strategies for reduc-ing the risk of global spread of infectious diseases through international trade.

Projects: •Studies on the mechanism of establishment and persistence of equine arteritis virus in the reproductive tract of the stallion.•Characterizing the site(s) of localization of equine arteritis virus in the carrier stallion.•Investigating the variation in virulence among naturally occurring strains of equine arte-ritis virus.•Developing a less costly, more rapid diagnostic test for EVA.•Developing a second generation marker vaccine for EVA/equine arteritis virus infection.•Developing a more rapid and reliable diagnostic test for detection of Taylorella equigenitalis/T. asinigenitalis. •Improving control and prevention strategies for contagious equine metritis (CEM).•Study of the epidemiology of Taylorella asinigenitalis in horses and non-horse equids.

Services:•Responsible for OIE Reference laboratory for equine viral arteritis and equine rhinopneu-monitis at the Gluck Center.•Provision of EVA diagnostic reagents to diagnostic laboratories, nationally and interna-tionally.•Provide consultation for veterinarians and members of the horse industry on various equine infectious diseases, including EVA, equine rhinopneumonitis, contagious equine metritis and equine piroplasmosis.•Co-editor of the Lloyd’s Equine Disease Quarterly.•Provide quarterly reports to the International Collating Centre, Animal Health Trust, Newmarket, UK, on equine infectious disease occurrences in the United States.•Veterinary advisor to the Thoroughbred Owners and Breeders Association (USA).

Graduate students, research staff and visiting scientists:•Yun Young Go, PhD (graduated 2011) – Molecular and genomic approaches to under-standing EAV pathogenesis•Jessica Hennig, MS Candidate – Studies on Equine Arteritis Virus: seasonal influences on viral shedding and neutralizing activity in semen of carrier stallions, vaccinal prophylaxis of yearling colts, diagnostic features of virus related abortion and prevalence of infection in camelids •Luisa Marenzoni, DVM, PhD, Visiting Scientist from Italy•Barry Meade, PhD Candidate – Comparisons of the transmission dynamics of disease outbreaks attributable to neuropathogenic and non-neuropathogenic strains of EHV-1 in closed populations•Jane Morrell, PhD, FRCVS, Visiting Scientist from Sweden•Cecilia Perglione, DVM,Visiting Scientist from Argentina•Kathleen Shuck, Research Analyst Principal

Peter Timoney, MVB, PhD, FRCVS

Frederick Van Lennep Chair in Equine

Veterinary Science,OIE Designated Specialist on Equine Viral Arteritis

& Equine Rhinopneumonitis


INFECTIOUS DISEASES

RESEARCH SNAPSHOTArticular cartilage maturation and repairGenomicsOsteoarthritisWobbler Syndrome

FACULTYJames MacLeod, Professor

Education: Fellowship – University of Pennsylvania (Endocrinology & Genetics), 1992PhD – University of Pennsylvania, 1990VMD – University of Pennsylvania, 1984BS – University of Delaware, 1980 Interest: The laboratory studies biological and biomedical aspects of the mus-culoskeletal system, with an emphasis on the growth and maturation of articular cartilage, the development of osteoarthritis, repair of articular lesions and the effects of anti-inflammatory medications. Experiments are conducted primarily on a cellular and molecular level. In addition to articular cartilage, areas of interest include cervical stenotic myelopathy (Wobbler Syndrome), genomics and limb development.

Projects: •Articular cartilage maturation.•Articular cartilage repair.•Intra-articular glucocorticoid therapy.•Tendon maturation.•Wobbler syndrome (cervical stenotic myelopathy).•Genomics and gene expression.

Postdoctoral/Graduate students and visiting scientists:•Stephen Coleman, PhD (graduated, 2011) – Analysis of the equine tran-scriptome by mRNA sequencing, Current Position: Postdoctoral Scholar•Rebekah Cosden, PhD (graduated, 2011) – Ambystoma mexicanium: A novel vertebrate model for diathrodial joint development and articular cartilage repair •Matthew Hestand, Postdoctoral Scholar – Equine transcriptome•Jennifer Janes, PhD Candidate – Pathogenesis of cervical stenotic myelopathy •Juipeng Liu, MS, Visiting Scientist from China – Articular cartilage maturation and repair •Parvathy Thampi, PhD Candidate – Articular cartilage maturation and repair •Ellen Wiegand, PhD Candidate – Articular cartilage maturation and repair •Wenying Zhu, PhD Candidate – Osteoarthritis and intra-articular gluco-corticoid therapy

James MacLeod, VMD, PhDProfessor


MUSCULOSKELETAL SCIENCES

RESEARCH SNAPSHOTDrug resistance in parasitesEquine protozoal myeloencephalitisHelminths, including life cycles, prevalence and control

FACULTYDaniel Howe, Associate ProfessorEugene Lyons, ProfessorMartin Nielsen, Assistant Professor

Education: PhD – Purdue University (Molecular Parasitology), 1992MS – Western Illinois University (Biology/Parasitology), 1990BS – Western Illinois University (Biology), 1988 Interest: Molecular studies of protozoan parasites – The primary research goal is to obtain a better understanding of the parasite Sarcocystis neurona, the primary cause of equine protozoal myeloencephalitis (EPM). Studies are ongoing to determine the genome sequence for S. neurona. Other interests include the development of improved serum assays for EPM diag-nosis. Additionally, we are investigating approaches to develop an effective vaccine against EPM.

Projects: •Characterization of novel genes and antigens from the parasite Sarcocystis neurona.•Development of a serologic assay for diagnosis of EPM and to develop a vaccine for EPM.•Sequencing and annotation of the S. neurona genome.

Postdoctoral/Graduate students, research staff and undergraduates:•Sriveny Dangoudoubiyam, Postdoctoral Scholar – Genome of Sarcocystis neurona•Breanna Gaubatz, MS Candidate – Genetic analysis of EPM horses•Ablesh Gautam, PhD Candidate – Characterization of the SnSAG family of surface anti-gens in Sarcocystis neurona •Xiao Xiao, MS Candidate – Analysis of serum antibodies against Sarcocystis neurona in foals•Michelle Yeargan, Research Specialist Undergraduate: Zijing Zhang

Daniel Howe, PhDAssociate Professor

Education: PhD – Colorado State University (Parasitology), 1963MS – Kansas State University (Parasitology), 1958BS – South Dakota State University (Wildlife), 1956 Interest: Parasitology: Control and transmission of internal parasites of horses. Nearly all dewormers currently on the market were tested for efficacy on internal parasites here in the Department of Veterinary Science. Studies are ongoing on resistance of small strongyles and ascarids to commercial dewormers. Other research is concentrated on profiling the passage of small strongyle eggs in feces (EPGs) of older horses. This is to try and determine which animals are low egg shedders, and thus don’t need deworming, and which ones are “high” egg shed-ders, which are needing antiparasitic treatment. Other research is on internal parasites of wildlife, especially hookworms in pinnipeds. Projects: •Control, transmission and prevalence of natural infections of internal parasites of horses.•Drug resistant nematodes in field and critical/controlled tests.

Eugene Lyons, PhDProfessor


PARASITOLOGY

Fluorescently-labeled Sarcocystis neurona para-sites (green) adjacent to the infected cell’s nucleus (blue). (Photo: Dan Howe)

Bot (Gasterophilis intestinalis) insect larvae of horses. First, second and third instars found in the mouth and/or stomach (on left) and first instar in an egg attached to a horse hair (on right). (Photos: Gene Lyons)

•Seasonal and yearly transmission.•Molecular identification of parasite species and basis of drug resistance.

Research staff and visiting scientists: •Tetiana Kuzmina, PhD, Visiting Scientists from Ukraine •Sharon Tolliver, Research Specialist

Education:Dip, EVPC – European Veterinary Parasitology College, 2011PhD – University of Copenhagen (Equine Parasitology), 2007DVM – Royal Veterinary and Agricultural University, 2001

Interests:Diagnosis and control of gastrointestinal helminths of horses and anthelmintic resistance. Studies will aim at 1) understanding and developing new diagnostic measures of important parasites and their level of resistance to anthelmintic drugs, 2) developing and evaluating sustainable anthelmintic treatment regimens using objective measures, and 3) applying molecular approaches for understanding mechanisms for development of anthelmintic resistance.

Projects: •Prepatent diagnosis of Strongylus vulgaris.•In vitro diagnosis of anthelmintic resistance.•The interaction between nematode parasites and the equine immune system.

Graduate student:Ulla Andersen, PhD Candidate – Prepatent diagnosis of Strongylus vulgaris

Martin Nielsen, DVM, PhD, DipEVPC

Assistant Professor


PARASITOLOGY

Education: DABT – Diplomate, American Board of Toxicology, 1980PhD – University of Toronto (Pharmacology), 1970MSc – University of Guelph (Pharmacology), 1966 MVB – University College, Dublin, 1964

Contributions: 1981: Drugs and the perforance horse, 480 page text. 1983: Regulatory threshold for furosemide. 1985-Present: About 100 ELISA tests for equine medications, licensed to Neogen Corp. 1997: US Patent for the first FDA approved treatment for Equine Protozoal Myelitis, licensed to Bayer, marketed as Marquis® and Protazil. 2003: US Copyright on unique biological mechanism of MRLS. 2006: US Patent for novel veterinary tranquilizer. 2011: Certified reference and internal standards for therapeutic medication regulation, licensed to Frontier Biopharm LLC. 2012: World medication rules, 289 page book.

Projects:•Ongoing research on certified reference standards and internal standards licensed to Neo-gen Corp. •Creation of ELISA tests for drug detection and certified reference standards for therapeu-tic medication regulation.•Developing animal models of ocular, fetal and central nervous system parasitic disease and demonstrating the therapeutic efficacy of specific chemotherapeutics. •Develop improved assays for ergot alkaloid analysis, the group of toxins involved in fescue toxicosis.

Postdoctoral student and research staff:•Charlie Hughes, Research Associate•Sucheta Kudrimoti, Postdoctoral Scholar – Relationships between medication or medica-tion residue concentrations and pharmacological effects in the contexts of resulting thera-peutic responses and/or the regulatory significance of medication residues with respect to competitive events.

The substance above is Myo-Inositol TrisPyroPhosphate, better known as ITPP. ITPP is considered to bind to hemoglobin and displace oxygen into muscles, thereby improving athletic performance. To enable control of this agent we have synthesized ITPP and provided certified reference standards for this substance to racing laboratories around the world to enable them to develop forensic tests and regu-latory control of this substance in performance horses. (Photo: Thomas Tobin)

FACULTYThomas Tobin, Professor

Thomas Tobin, MVB, MSc, PhD, MRCVS, DABT

Professor of Veterinary Science and the Graduate

Center for Toxicology

RESEARCH SNAPSHOTTherapeutic medication regulationEstablishing world wide reference standards for therapeutic medications, dietary and environmental substances Developing novel ELISA-based forensic testsDevelopment of specific and sensitive tests to detect ergot alkaloids (associated with fescue toxicity and other diseases)Development of novel therapies for infectious and other diseases


PHARMACOLOGY/TOXICOLOGY

Education: Dipl. ACT – 1987PhD – Cornell University, 1987DVM – University of Georgia, 1981

Projects:•Studies on anti-Müllerian hormone in the mare and stallion.•Down regulation of oxytocin receptors and luteal maintenance in mares.•Bioactivity of 5 α-dihydroprogesterone in mares.•Diagnostic methods related to placentitis and late abortion in mares.•Nonsurgical control of reproductive behavior in the stallion.

Postdoctoral/Graduate students:•Anthony Claes, DVM, Dipl. ACT, PhD Candidate – Studies on anti-Müllerian hormone in the mare and stallion•Igor Canisso, DVM, Dipl. ACT, PhD Candidate – Studies on placentitis and late abortion in mares•Alejandro Esteller-Vico, DVM, PhD, Postdoctoral Scholar – Studies on endocrinology and sperm biology in the horse•Lauren Keith, MS Candidate – Suppression of estrous in race mares

Barry Ball, DVM, PhD, Dipl. ACTProfessor and Albert G. Clay Endowed Chair in Equine Reproduction

Karen McDowell, PhD, EMB

Associate Professor

Education: EMB – Certified Embryologist, American College of Embryology, 2010NIH Postdoctoral Fellow – Colorado State University (Physiology), 1987PhD – University of Florida (Animal Science), 1986MS – University of Tennessee (Animal Science), 1980BS – University of Tennessee (Animal Science), 1976 Interest: Determining causes of reproductive losses in mares, including maternal-embryo or ma-ternal-fetal interactions, mare reproductive loss syndrome, and most currently, effects of endophyte-infected fescue on pregnant mares. Overall goal is to reduce pregnancy losses and enhance pregnancy maintenance in mares.

Projects: •Vascular changes associated with consumption of endophyte-infected fescue.•In vitro assessment of ergot alkaloids, receptor agonists and receptor antagonists on equine peripheral and central arteries.

Graduate student:•Drew Hestad, MS Candidate – Equine fescue toxicosis


RESEARCH SNAPSHOTCauses, diagnosis and treatment of embryonic and fetal loss in mares Early embryonic development Uterine infectionNutritional affects on reproductionStallion behaviorDiagnosis and treatment of fertility problems in stallionsFescue toxicosis Frozen stallion semen

FACULTYBarry Ball, ProfessorKaren McDowell, Associate ProfessorEd Squires, Professor Tom Swerczek, ProfessorMats Troedsson, Professor

REPRODUCTIVE HEALTH

Edward Squires, MS, PhD, Dipl. ACT (hon.)

Professor, Director of UK Ag Equine Programs and

Executive Director of the Gluck Equine

Research Foundation

Education: Dipl. ACT (hon.) – 2003PhD – University of Wisconsin, 1974MS – West Virginia University, 1971BS – West Virginia University, 1969 Interest: Improving the reproductive efficiency of both mares and stallions, hormonal control of the cycle and development of reproductive techniques. Projects: •Examination of farm records: performance of surviving foals out of mares treated for placentitis and incidence and outcomes of dystocia (Hughes)•Potential transfer of bacteria from infected stallions to mares–does this occur and then affect pregnancy rates? (Cerny)•Density gradient centrifugation of frozen semen (Hughes and Cerny)•Induce twin pregnancy to double eCG levels (Hughes)•Uterine rupture in mares (Toro Mayorga)•Establishment of sustained EAV infection (Campos) •Factors affecting freezability of stallions (Kalmar)

Graduate students:•Katheryn Cerny, MS Candidate – Transmission of bacteria from the stallion to the mare and its effect on pregnancy rate and embryonic losses•Sydney Hughes, MS Candidate – Reproductive problems in late pregnancy mares•Julianne Kalmar, MS Candidate – Factors affecting freezability of stallions

Education: PhD – University of Connecticut (Comparative Pathology), 1969 MS – University of Connecticut (Nutritional Pathology), 1966 DVM – Kansas State University, 1964BS – Kansas State University, 1962 Interest: Nutritional Pathology. Factors that cause abortion in mares, including climatic and envi-ronmental changes that induce stress to pasture forages. Drought, excessive rainfall, frosts and freezes can induce nutrient imbalances.

Projects: •Evaluation of bacterial endophytes of grass-and-legume forages as emerging causes of reproductive loss in horses.•Develop diagnostic tests for tissues and blood to aid in diagnosis of fetal loss.

Tom Swerczek, DVM, PhDProfessor


REPRODUCTIVE HEALTH

Education: Dipl. ECAR – 2002Dipl. ACT – 1993 PhD – University of California – Davis, 1991DVM – Royal Veterinary College (Stockholm, Sweden), 1975

Interest:Equine reproductive health and biology. 1) The interaction between sper-matoza and the uterine immune system with a particular note on its role in breeding-induced endometritis. 2) The role of seminal proteins in fertility. 3) The interaction between the mares’ uterus and the early conceptus during the critical time of pregnancy recognition. 4) Diagnostics and treatment of high-risk pregnancies.

Projects:•Interaction between semen and the uterus in horses.•Identification of seminal plasma proteins that affect fertility.•Inflammation of the uterus – role of nitric oxide and inflammatory cytokines in infectious and breeding-induced endometritis. •Causes, diagnostics and control of high-risk pregnancies.

Postdoctoral/Graduate students, research staff and visiting scientists: •Juliana Campos, MS Candidate – EVA/disease resistance•Alejandro Esteller-Vico, DVM, PhD, Postdoctoral Scholar – Seminal plasma proteins•Claudia Klein, Postdoctoral Scholar – Early pregnancy recognition/genomics and gene expression and regulation in reproductive biology•Ana Gabriella Toro Mayorga, MS Candidate – Uterine pathology/biopsies•Kirsten Scoggin, PhD, Senior Scientist •Elizabeth Woodward, PhD Candidate – Endometritis

Mats Troedsson, DVM, PhD, Dipl. ACT, Dipl. ECAR

Professor, Director of the Gluck Equine Research Center, and Chair of the Department of Veterinary Science


REPRODUCTIVE HEALTH

Gluck Equine Research Center

Competitive Grant Funding

Non-Competitive Grant Funding

The mission of the Gluck Center is scientific discovery, education and dissemination of knowledge for the benefit of the health and

well-being of horses.

Adams, A.A. 2011. LAND O’LAKES PURINA FEED LLC. A New Formulation of Purina® Equine Senior® Feed: The effect on inflammatory cytokine production, oxidative status and im-mune responses to vaccination in old horses. (Co-PI: Horohov, D.W.)

Adams, A.A. 2011. AQHA Foundation. Identifying the role of a ‘metabolic master switch’ in Equine Metabolic Syndrome and its implications for targeted treatment. (Co-PI: Horohov, D.W.)

Adams, A.A. 2011. USDA-ARS. The effect of Endophyte-Infected Tall Fescue consumption on epigenetic regulation of inflammation in horses. (Co-PI: Horohov, D.W.)

Artiushin, S.C. 2010-2011. Grayson-Jockey Club Research Foundation. Toxins TcD A and B of Clostridium difficile for horse immunization. (Co-PI: Timoney, J.F.) $45,885.

Balasuriya, U.B.R. 2010-2012. Grayson-Jockey Club Re-search Foundation, Inc. Molecular characterization of neuro-virulent EHV-1 strains. (Co-PI: Cook, R.F. and Timoney, P.J.) $95,361.

Balasuriya, U.B.R. 2010-2011. NIH-R13 (PA-10-071). XIIth International Nidovirus Symposium Support Proposal. $12,000.

Balasuriya, U.B.R. 2011-2012. Morris Animal Foundation (Pilot Study). Identification of Genetic Factors Responsible for Establishment of EAV Carrier State in Stallions. $10,800.

Balasuriya, U.B.R. 2011-2012. Department of Homeland Se-curity (DHS) through Center of Excellence for Emerging and Zoonotic Animal Diseases (CEEZAD) Cooperative Agreement 2010-ST-061-AG0001, Kansas State University, Manhattan, KS. Rapid detection of foreign, emerging and zoonotic patho-gens of equines. $40,000.

Balasuriya, U.B.R. 2011. AFRI-USDA (Matching funds will be provided by the University of Kentucky College of Agricul-ture). Acquisition of a FluorChem E Digital Imaging System for Arterivirus (PRRSV and EAV) Vaccine Research. $13,375.

Balasuriya, U.B.R. 2010-2011. AFRI-USDA. Funding Re-quest for XIIth International Nidovirus Symposium (Nido2011). $13,000.

Chambers, T.M. and Horohov, D.W. 2010-2011. Further char-acterization of the immunological response of horses to vacci-nation with Fluvac Innovator. Contract. $88,802.

Chambers, T.M. 2011-2012. American Quarter Horse Founda-tion. Equine herpesvirus-1 and the Type-I interferon response. (Co-PI: Horohov, D.W.) $37,236.

Chambers, T.M. 2011-2013. Kentucky Science and Engineer-ing Foundation. Role of the horse in interspecies transmission of influenza viruses. (Co-PI: Balasuriya, U.B.R.) $90,000.

Competitive Grant Funding

Funding is important to equine research. The faculty at the Gluck Equine Research Center were success-ful in obtaining several competitive and non-competitive grants, some of which were multi-year grants.

Cook, R.F., D.W. Horohov, C. Issel. 2009-2014. NIH. EIAV envelope variation and vaccine efficacy. Co-PIs (PI: Montelaro, R.), $1,737,500 ($3,668,813 total).

Dwyer, R.M. 2009-2011. Extension Disaster Education Net-work with CSREES funding. The EDEN Strengthening Agros-ecurity Planning Train-the-Trainer Program. Co-PI (PI: Hus-band, A. Co-PIs: Yeargan, R. and Newman M.), $75,294.

Horohov, D. W. 2011. Morris Animal Foundation. Determin-ing the role of maternal antibodies in infection and immunity to Lawsonia intracellularis.

Horohov, D. W. 2009-2011. USDA-AFRI. The effect of age on equine dendritic cell interactions with Rhodococcus equi. $200,000.

Horohov, D.W. 2010-2011. Equine Drug Research Council. Seroprevalence of Lawsonia intracellularis in central Ken-tucky Thoroughbred weanlings. $45,841.

Horohov, D.W. and E.L. Lyons. 2010-2011. Pfizer Animal Health. Characterization of the inflammatory response to an-thelmintics. Contract. $38,473.

Horohov, D.W. 2010-2011. Pfizer Animal Health. Further characterization of the immunological response of horses to Metastim. Contract. $48,193.

Howe, D.K. 2009-2012. USDA/CSREES. Genome Sequence for the apicomplexan Sarcocystis neurona. $500,000.

Lear, T. 2009-2011. National Institutes of General Medical Sciences, National Institutes of Health. Behavioral genomics of the White-Throated sparrow. Co-PI. (E. Tuttle, PI, Indiana State University). $84,000.

MacLeod, J.N. 2009-2011. Morris Animal Foundation. Ortho-paedic pathology and genetic associations in cervical stenotic myelopathy. Sponsor for Jennifer Janes, $100,000.

MacLeod, J.N. 2009-2012. National Science Foundation. Exon splice pattern characterization of the whole mRNA tran-scriptome. Co-PI (PI: Liu, J.). $1,027,384.

MacLeod, J.N. 2010-2012. Kentucky Horse Racing Commis-sion. Safety and anti-inflammatory efficacy of glucocorticoids for intra-articular therapy in racehorses. PI. $99,969.

MacLeod, J.N. 2010-2012. Grayson-Jockey Club Foundation. Orthopaedic Pathology and Genetic Associations with Cervical Stenotic Myelopathy. PI. $102,193.

MacLeod, J.N. 2011-2013. Kentucky Horse Racing Commis-sion. Articular Cartilage Stem Cells. PI. $100,000.

McDowell, K.J., L. Lawrence, G. Aiken, L. Bush, D.W. Horo-


Gluck Equine Research Center Grants

Non-Competitive Grant FundingBalasuriya, U.B.R. 2009-2011. Paul Mellon Post Doctoral Scholarship Award to Dr. Yanqiu Li. Comparative Genomics of Gamma-Herpesviruses of Equids. PI and author. $72,000.

Balasuriya, U.B.R. 2009-2012. Shapiro Endowment. Estab-lish correlation between in vitro susceptibility of CD3+ lym-phocytes to EAV infection and the establishment of persistent infection in stallions. (Co-PI: E. Bailey, E.L. Squires, P.J. Timoney, M.H.T. Troedsson). $25,000.

Balasuriya, U.B.R. 2010-2012. Koller Endowment. Laborato-ry investigation into an extensive respiratory disease outbreak in Thoroughbred breeding farms in Lexington, Kentucky. (Co-PI: D.W. Horohov and R.F. Cook) $20,000.

Cook, R.F. 2011. Clay Fellowship Fund, Gluck Equine Re-search Center, to support Dr. Michelle Quinlivan, Irish Equine Centre. Infectivity and Pathogenicity of Full Length Molecular Clones Derived From Equine Infectious Anemia Virus (EIAV) Strains Isolated in Ireland During 2006. $15,355.

Dwyer, R.M., N. Williams, P.J. Timoney. 2011. Lloyd’s of London. Continued funding for the Lloyd’s Equine Disease Quarterly. $45,000.

Horohov, D.W., J.F. Timoney, A. Page, A. Loynachan, U. Bryant. Kohler Funds for newly emerging equine diseases. De-velopment of an infectious model for equine proliferative en-teropathy caused by Lawsonia intracellularis. $21,168.

Horohov, D.W. and A.A. Adams. Gluck Equine Research Center. The determination of telomerase activity and its rela-

hov. 2007-2013. USDA-ARS-FAPRU. Vascular Changes As-sociated with Consumption of E+ Tall Fescue by Broodmares. $187,044.

McDowell, K.J. USDA-ARS-FAPRU-SCA. Consumption of endophyte infected (E+) tall fescue seed by mares will cause vasoconstriction that can be measured by color-Doppler and real-time (B-mode) ultrasonography.

Squires, E.L. 2010-2011. Kentucky Equine Drug and Racing Commission. Estrus suppression in the mare. (Co-PI: Troeds-son, M.H.T.). $30,000.

Squires, E.L. 2011. Dogwood Foundation. Using genetic tech-nology to determine the fertility of stallion semen. (Co-PI: E. Bailey, B.A. Ball, M.H.T. Troedsson). $25,000.

Timoney, J.F. 2009-2012. Hatch Project. #04-01. Identification

tionship with telomere length, oxidative stress and inflamma-tion. $9,630.

MacLeod, J.N. 2010-2014. Lourie Foundation. Cellular Mech-anisms of Articular Cartilage, Tendon, and Ligament Repair. PI. $500,000.

Perkins, G., K. Osterreider and J.F. Timoney. 2012. EHV-1 strain RacH as a vaccine vector of SeM, Se18.9 and IdeE pep-tides of Streptococcus equi. $15,000.

Squires, E.L. 2010-2011. Bacteria found in semen and repro-ductive tracts of stallions and its relation to mare fertility using natural service. (Co-PI: M.H.T. Troedsson)

Squires, E.L. 2010-2011. Performance of foals from mares treated for placentitis during late gestation. (Co-PI: M.H.T. Troedsson)

Squires, E.L. 2010-2011. Effect of season, age and reproduc-tive status at time of breeding on occurrence of dystocia. (Co-PI: M.H.T. Troedsson)

Squires, E.L. 2010-2011. The role of nitric oxide in equine post breeding endometritis. Co-PI.

Squires, E.L. 2010-2011. Relationship of endometrial angi-opathies to uterine artery rupture. Co-PI.

Tobin, T. 2009-2011. National and Local Horsemen’s Benevo-lent and Protective Associations. Synthesis of certified refer-ence standards. $91,673.

of surface and secreted proteins of Streptococcus equi with po-tential in vaccine development. (Co-PI: Artiushin, S.C.).

Timoney, J.F. and S. Velineni. Equine Drug Research Council. Evaluation of novel immunoreactive proteins of Streptococcus zooepidemicus for potential as vaccine components. $48, 171.

Tobin, T. 1995-Present. National Horsemen’s Benevolent and Protective Association. Synthesis of Certified Reference Stan-dards. Current support: $100,000.

Tobin, T. 2008-2011. KY Commercialization Fund Proposal. Early diagnosis of fescue toxicity. PI, $149,000.

Troedsson, M.H.T. July 2011-Dec. 2012. Kentucky Thorough-bred Association. Equine Placentitis: Development of a mod-el to study nocardioform placentitis. (Co-PI: E.L. Squires). $41,995.


Gluck Equine Research Center Grants

Phot

o by

Gay

le E

wad

inge

r

Scientific Publications

Books/Chapters in Books

Refereed Journal Articles

Non-Refereed Articles

Presentations/Meetings Attended

Bailey, E. 2011. Parentage testing. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduc-tion, 2nd Ed. Wiley-Blackwell Publishing, Chapter 297, pp. 2820-2826.

Bailey, E. 2011. Relevance of genomics to equine reproduction. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduction, 2nd Ed. Wiley-Blackwell Publishing, Chapter 298, pp. 2827-2831.

Ball, B.A. 2011. Oxidative stress in sperm. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduction, 2nd Ed. Wiley-Blackwell Publishing, Chapter 98, pp. 991-995.

Ball, B.A. 2011. Sperm-oviduct interactions. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduction, 2nd Ed. Wiley-Blackwell Publishing, Chapter 103, pp. 1085-1091.

Ball, B.A. 2011. Embryonic loss. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduc-tion, 2nd Ed. Wiley-Blackwell Publishing, Chapter 239, pp. 2327-2338.

Ball, B.A. 2011. Oxidative stress in sperm. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduction, 2nd Ed. Wiley-Blackwell Publishing, Chapter 207, pp. 1951-1962.

Lear, T.L., and D.A.F. Villagomez. 2011. Cytogenetic evaluation of mares and foals.

Lyons, E.T., M. Ionita, and S.C. Tolliver. 2011. Important gastrointestinal parasites. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduction, 2nd Ed. Wiley-Blackwell Publishing, Chapter 30, pp. 292-301.

McKinnon, A., E.L. Squires, W. Vaala, and D. Varner. 2011. Equine Reproduction, Second Edition. Wiley-Blackwell.

Powell, D.G. 2011. Mare reproductive loss syndrome. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduction, 2nd Ed. Wiley-Blackwell Publishing, Chapter 248, pp. 2410-2417.

Squires, E.L., and B.W. Pickett. 2011. Factors affecting sperm production and output. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduction, 2nd Ed. Wiley-Blackwell Publishing, Chapter 133, pp. 1344-1362.

Squires, E.L. 2011. Reproduction parameters from light horse stallions. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduction, 2nd Ed. Wiley-Blackwell Publishing, Chapter 135, pp. 1367-1376.

Squires, E.L. 2011. Progesterone. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduc-tion, 2nd Ed. Wiley-Blackwell Publishing, Chapter 184, pp. 1778-1982.

Squires, E.L. 2011. Gonadotropin releasing hormones. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduction, 2nd Ed. Wiley-Blackwell Publishing, Chapter 190, pp. 1820-1824.

Squires, E.L., and P.M. McCue. 2011. Superovulation. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduction, 2nd Ed. Wiley-Blackwell Publishing, Chapter 193, pp. 1836-1845.

Timoney, J.F. 2011. Strangles. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduction, 2nd Ed. Wiley-Blackwell Publishing, Chapter 77, pp. 704-709.

Research results conducted by the faculty at the Gluck Center was published in various forms throughout 2011, including books or chapters in books, refereed journal articles and non-refereed articles.

Books/Chapters in Books



Refereed Journal ArticlesAdams, A.A., T.L. Sturgill, C.C. Breathnach, T.M. Chambers, L. Siger, J.M. Minke, and D.W. Horohov. 2011. Humoral and cell-mediated immune responses of old horses following recombinant canarypox virus vaccination and subsequent chal-lenge infection. Vet Immunol Immunopathol 139:128-140.

Almeida, J., A.J. Conley, L. Mathewson, and B.A. Ball. 2011. Expression of steroidogenic enzymes during equine testicular development. Reproduction 141:841-848.

Almeida, J., B.A. Ball, A.J. Conley, L. Mathewson, N.J. Place, I.K.M. Liu, E.L. Scholtz, S.D. Stanley, and B.C. Moeller. 2011. Biological and clinical significance of anti-Müllerian hormone determination in blood serum of the mare. Therio-genology 76:1393-1403.

Artiushin, S., Y. Tong, J. Timoney, B. Lemieux, A. Schlegel, and H. Kong. 2011. Thermophilic helicase-dependent DNA amplification using the IsoAmp™ SE experimental kit for rapid detection of Streptococcus equi subspecies equi in clinical samples. Journal of Veterinary Diagnostic Investigation 23(5):909-914.

Binns, M., D. Boehler, E. Bailey, T. Lear, J. Cardwell, D. Lambert. 2011. Inbreeding in the Thoroughbred horse. Animal Genetics (online: 8 NOV 2011 DOI: 10.1111/j.1365-2052.2011.02259.x).

Broaddus, C.C., U.B.R. Balasuriya, J. Richards, P.J. Timoney, R.A. Funk, and G.R. Holyoak. 2011. Evaluation of the safety of vaccinating mares against equine viral arteritis in mid or late pregnancy or during the immediate postpartum pe-riod. JAVMA 238:741-750.

Broaddus, C.C., U.B.R. Balasuriya, J. White, P.J. Timoney, C. Makloski, K. Torrisi, and G.R. Holyoak. 2011. Infection of embryos following insemination of donor mares with equine arteritis virus infective semen. Theriogenology 76(1):47-60.

Bryant, N.A., A.S. Rash, A.L. Woodward, E. Medcalf, M. Helwegen, F. Wohlfender, F. Cruz, C. Herrmann, K. Borchers, A.

Timoney, P.J. 2011. Equine herpesvirus. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduction, 2nd Ed. Wiley-Blackwell Publishing, Chapter 245, pp. 2376-2390.

Timoney, P.J. 2011. Equine viral arteritis. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduction, 2nd Ed. Wiley-Blackwell Publishing, Chapter 246, pp. 2391-2398.

Timoney, P.J. 2011. Contagious equine metritis. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduction, 2nd Ed. Wiley-Blackwell Publishing, Chapter 247, pp. 2399-2409.

Timoney, P.J. 2011. Diseases potentially transmissible with frozen or cooled semen. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Varner (eds.) Equine Reproduction, 2nd Ed. Wiley-Blackwell Publishing, Chapter 321, pp. 3015-3028.

Timoney, P.J. 2011. Equine viral arteritis. In: The Merck Veterinary Manual, 10th Ed., C.M. Kahn (ed.), Merck and Co., Inc., Whitehouse Station, NJ, pp. 633-642.

Troedsson, M.H.T. 2011. Endometritis. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D. Varner (eds.) Equine Repro-duction, 2nd Ed. Wiley-Blackwell Publishing, Chapter 271, pp. 2608-2619.

Troedsson, M.H.T., and M.L. Macpherson. 2011. Placentitis. In: McKinnon, A.O., E.L. Squires, W.E. Vaala, and D.D. Var-ner (eds.) Equine Reproduction, 2nd Ed. Wiley-Blackwell Publishing, Chapter 242, pp. 2359-2367.

Troedsson, M.H.T., and P.J. Timoney. 2011. Contagious Equine Metritis. In: Wilson, D.A. (ed.), Clinical Veterinary Advi-sor: The Horse, Saunders Elsevier, pp. 124-126.



Tiwari, T.M. Chambers, J.R. Newton, J.A. Mumford, D.M. Elton. 2011. Isolation and characterisation of equine influenza viruses (H3N8) from Europe and North America from 2008 to 2009. Veterinary Microbiology 147:19-27

Cappelli, K., S. Capomaccio, R.F. Cook, M. Felicetti, M.L. Marenzoni, G. Coppola, A. Verini-Supplizi, M. Coletti and F. Passamonti. 2011. Molecular detection, epidemiology and genetic characterization of novel European field isolates of equine infectious anemia virus. Journal of Clinical Microbiology 49:27-33.

Casagrande Proietti, P., A. Bietta, G. Coppola, M. Felicetti, R.F. Cook, M. Coletti, M.L. Marenzoni and F. Passamonti. 2011. Isolation and characterization of β-haemolytic-Streptococci from endometritis in mares. Veterinary Microbiology 152:126-130.

Cerny, K.L., S. Hughes, J.R. Campos, M.H.T. Troedsson, E.L. Squires. 2011. Fertility of mares inseminated with frozen/thawed semen centrifuged through a single layer density gradient. Journal of Equine Veterinary Science. 31:316-317.

Cosden, R.S., C. Lattermann, S. Romine, J. Gao, S.R. Voss, and J.N. MacLeod. 2011. Intrinsic repair of full-thickness ar-ticular cartilage defects in the axolotl salamander. Osteoarthritis and Cartilage 19:200-205.

Dangoudoubiyam, S., J.B. Oliveira, C. Víquez, A. Gómez-García, O. González, J.J. Romero, O.C.H. Kwok, J.P. Dubey, and D.K. Howe. 2011. Detection of antibodies against Sarcocystis neurona, Neospora spp., and Toxoplasma gondii in horses from Costa Rica. Journal of Parasitology 97(3):522-524.

DeLuca, C.A., P.A. McCue, M.L. Patten, and E.L. Squires. 2011. Effect of a nonsurgical embryo transfer procedure and/or altrenogest therapy on endogenous progesterone concentration in mares. Journal of Equine Veterinary Science 31:57-62.

Doty, A, W.C. Buhi, S. Benson, K.E. Scoggin, M. Pozor, M. Macpherson, M. Mutz, and M.H.T. Troedsson. 2011. Equine crisp3 modulates interaction between spermatozoa and polymorphonuclear neutrophils. Biology of Reproduction 85(1):157-64.

Firth, A.E., J.C. Zevenhoven-Dobbe, N.M. Wills, Y.Y. Go, U.B.R. Balasuriya, J.F. Atkins, E.J. Snijder, and C.C. Posthuma. 2011. Discovery of a small arterivirus gene that overlaps the GP5 coding sequence and is important for virus production. Journal of General Virology 92(5):1097-1106.

Fog, P., H. Vigre, and M.K. Nielsen. 2011. Strongyle egg counts in Standardbred trotters: Are they associated with race performance? Equine Veterinary Journal 43:89-92.

Furr, M., Howe, D., Reed, S., and Yeargan, M. 2011. Antibody coefficients for the diagnosis of Equine Protozoal Myeloen-cephalitis. Journal of Veterinary Internal Medicine 25:138-142.

Gautam, A., J.P. Dubey, W.J. Saville, and D.K. Howe. 2011. The SnSAG merozoite surface antigens of Sarcocystis neurona are expressed differentially during the bradyzoite and sporozoite life cycle stages. Veterinary Parasitology 183:37-42.

Go, Y.Y., E. Bailey, D.G. Cook, S.J. Coleman, J.N. MacLeod, K.C. Chen, P.J. Timoney and U.B.R. Balasuriya. 2011. Genome-wide association study among four horse breeds identifies a common haplotype associated with in vitro CD3(+) T cell susceptibility/resistance to equine arteritis virus infection. Journal of Virology 85(24):13174-13184.

Go, Y.Y., E.J. Snijder, P.J. Timoney, and U.B.R. Balasuriya. 2011. Characterization of equine humoral antibody response to the nonstructural proteins of equine arteritis virus. Clinical and Vaccine Immunology 18:268-279.

Guiterrez, J., R. Eisenberg, G. Herrensmith, T. Tobin, and A.M. Craig. 2011. Solvatomorphism in (E)-2-(2,6-dichloro-4-hydroxybenzylidene) hydrazinecarboximidamide. Acta Chrystallographica Section C – Crystal Structure Communica-tions. 67:0310-0314.

Horohov, D.W., A.T. Loynachan, A.E. Page, K. Hughes, J.F. Timoney, M. Fettinger, T. Hatch, J.G. Spaulding, and J. Mc-Michael. 2011. The use of streptolysin O (SLO) as an adjunct therapy for Rhodococcus equi pneumonia in foals. Veterinary



Microbiology 154(1-2):156-162.

Hudgens, E., D. Tompkins, P. Boyd, J.K. Lunney, D. Horohov, and C.L. Baldwin. 2011. Expressed gene sequence of the IFNgamma-response chemokine CXCL9 of cattle, horses, and swine. Veterinary Immunology and Immunopathology 141:317-321.

Hussey, G.S.B., B. Wagner, D.W. Horohov, G.R. Van de Walle, N. Osterrieder, L.S. Goehring, S. Rao, and D.P. Lunn. 2011. Evaluation of immune responses following infection of ponies with an EHV-1 ORF1/2 deletion mutant. Veterinary Research 42:23.

Ijaz, M., S. Velineni, and J.F. Timoney. 2011. Selective pressure for allelic diversity in SEM of Streptococcus equi does not affect immunoreactive proteins SzPSe or Se18.9. Infection, Genetics and Evolution 11:1159-1163.

Kikuchi, M., Y. Nakano, Y. Nambo, S. Haneda, M. Matsui, Y. Miyake, J.N. MacLeod, K. Nagaoka, and K. Imakawa. 2011. Production of calcium maintenance factor Stanniocalcin-1 (STC1) by the equine endometrium during the early pregnant period. Journal of Reproduction and Development 57:203-211.

Klein, C., K.E. Scoggin, and M.H.T. Troedsson. 2011. The expression of interferon-stimulated gene 15 in equine endome-trium. Reproduction in Domestic Animals 46(4):692-698.

Kuzmina,T.A., S.C. Tolliver, and E.T. Lyons. 2011. Three recently recognized species of cyathostomes (Nematoda: Stron-gylidae) in equids in Kentucky. Parasitology Research 108:1179-1184.

Kyvsgaard, N.C., J. Lindbom, L.L. Andreasen, L.A. Luna-Olivares, M.K. Nielsen, and J. Monrad. 2011. Prevalence of stron-gyles and efficacy of fenbendazole and ivermectin in working in El Sauce, Nicaragua. Veterinary Parasitology 181:248-254.

Larsen M.L., C. Ritz, S.L. Petersen, M.K. Nielsen. 2011. Determination of ivermectin efficacy and egg reappearance period on horse farms using selective therapy. Veterinary Journal 188(1):44-47.

Lehner, A.F., J.M. Duringer, C.T. Estill, T. Tobin, and A.M. Craig. 2011. ESI-Mass spectrometric and HPLC elucidation of a new ergot alkaloid from perennial ryegrass hay silage associated with bovine reproductive problems. Toxicology Mecha-nisms & Methods 21(8):606-621.

Lehner, A.F., J.A. Hitron, J. May, C. Hughes, R. Eisenberg, N. Schwint, D.P. Knowles, P. Timoney, and T. Tobin. 2011. Evaluation of mass spectrometric methods for detection of the anti-protozoal drug imidocarb. Journal of Analytical Toxicol-ogy 35:199-204.

Lewis, N.S., J.M. Daly, C.A. Russell, D.L. Horton, E. Skepner, N.A. Bryant, A.S. Rash, J.A. Mumford, J.L.N. Wood, T.M. Chambers, R.A.M. Fouchier, M.D. Elton, D.J. Smith. 2011. The antigenic and genetic evolution of equine influenza A (H3N8) virus from 1968-2007. J. Virol. 83: 12742-12739.

Lindbom J., L.L. Andreasen, L.A. Luna-Olivares, J. Monrad, M.K. Nielsen, N.C. Kyvsgaard. 2011. Prevalence and anthel-mintic control of strongyles in working horses in Nicaragua. Veterinary Parasitology 181, 248-254.

Liu, C., A. Betancourt, D.A. Cohen, A.A. Adams, L. Sun, and D.W. Horohov. 2011. Granzyme B-mRNA expression by equine lymphokine activated killer (LAK) cells is associated with the induction of apoptosis in target cells. Veterinary Im-munology & Immunopatholology 143:108-115.

Lyons, E.T., R.L. DeLong, S.A. Nadler, J.L. Laake, A.J. Orr, B.L. DeLong, and C. Pagan. 2011. Investigations of peritoneal and intestinal infections of adult hookworms (Uncinaria spp.) in northern fur seal (Callorhinus ursinus) and California sea lion (Zalophus californianus) pups on San Miguel Island, California (2003). Parasitology Research 109:581–589.

Lyons, E.T., S.C. Tolliver, and S.S. Collins. 2011. Reduced activity of moxidectin and ivermectin on small strongyles in young horses on a farm (BC) in Central Kentucky in two field tests with notes on variable counts of eggs per gram of feces



Scientific Publications (EPGs). Parasitology Research 108:1315–1319.

Lyons, E.T., S.C. Tolliver, S.S. Collins, M. Ionita, T.A. Kuzmina, and M. Rossano. 2011. Field tests demonstrating reduced activity of ivermectin and moxidectin against small strongyles in horses on 14 farms in Central Kentucky in 2007–2009. Parasitology Research 108(2):355-360.

Lyons, E.T., S.C. Tolliver, T.A. Kuzmina, and S.S. Collins. 2011. Further evaluation in field tests of the activity of three anthelmintics (fenbendazole, oxibendazole, and pyrantel pamoate) against the ascarid Parascaris equorum in horse foals on eight farms in Central Kentucky (2009–2010). Parasitology Research 109:1193–1197.

Lyons, E.T., T.A. Kuzmina, S.C. Tolliver, and S.S. Collins. 2011. Observations on development of natural infection and species composition of small strongyles in young equids in Kentucky. Parasitology Research 109:1529-1535.

Lyons, E.T., T.R. Spraker, R.L. De Long, M. Ionita, S.R. Melin, S.A. Nadler, and S.C. Tolliver. 2011. Review of research on hookworms (Uncinaria lucasi Stiles, 1901) in northern fur seals (Callorhinus ursinus Linnaeus, 1758). Parasitology Re-search 109:257-265.

Lyons, E.T., S.C. Tolliver, T.A. Kuzmina. 2011. Investigation of strongyle EPG values in horse mares relative to known age, number positive, and level of egg shedding in field studies on 26 farms in Central Kentucky (2010–2011). Parasitology Research. Online First™. 2011 Dec. 14.

Lyons, E.T. Macrocyclic Lactones for Parasite Control in Equids. Curr Pharm Biotechnol. 2011 Oct. 31.

Mérant, C., A. Sheoran, and J.F. Timoney. 2011. Association of Streptococcus equi with equine monocytes. Veterinary Im-munology & Immunopathology 143(1-2):83-86.

Miszczak, F., L. Legrand, U.B.R. Balasuriya, B. Ferry-Abitbol, J. Zhang, A. Hans, G. Fortier, S. Pronost, and A. Vabret. 2011. Emergence of novel equine arteritis virus (EAV) variants during persistant infection in the stallion: origin of the 2007 French EAV outbreak was linked to an EAV strain present in the semen of a persistently infected carrier stallion. Virology 423:165-174.

Miszczak, F., K.M. Shuck, Z. Lu, Y.Y. Go, J. Zhang, S. Sells, A. Vabret, S. Pronost, G. Fortier, P.J. Timoney, and U.B.R. Balasuriya. 2011. Evaluation of two magnetic bead-based viral nucleic acid purification kits and three real-time RT-PCR reagent systems in two TaqMan assays for equine arteritis virus detection. Journal of Clinical Microbiology 49(10):3694-3696.

Natarajaseenivasan, K., K. Vedhagiri, V. Sivabalan, S.G. Prabagaran, S. Sukumar, S.C. Artiushin, and J.F. Timoney. 2011. Seroprevalence of Leptospira borgpetersenii serovar javanica infection among dairy cattle, rats and humans in the Cauvery River valley of southern India. Southeast Asian Journal of Tropical Medicine and Public Health. 42(3):679-686.

Natarajaseenivasan, K., S. Shanmughapriya, S. Velineni, S.C. Artiushin, and J.F. Timoney. 2011. Cloning, expression, and homology modeling of Gro EL protein from Leptospira interrogans Serovar Autumnalis strain N2. Genomics, Proteomics and Bioformatics 9:151-157.

Natarajaseenivasan, K., S.C. Artiushin, S. Velineni, K. Vedhagiri, P. Vijayachari, and J.F. Timoney. 2011. Surface-asso-ciated Hsp60 chaperonin of Leptospira interrogans serovar Autumnalis N2 strain as an immunoreactive protein. European Journal of Clinical Microbiology & Infectious Diseases 30(11):1383-1389.

Page, A.E., A.T. Loynachan, U. Bryant, H.F. Stills, Jr., A.A. Adams, C.J. Gebhart, N. Pusterla, and D.W. Horohov. 2011. Characterization of the interferon gamma response to Lawsonia intracellularis using an equine proliferative enteropathy challenge (EPE) model. Veterinary Immunology & Immunopatholology 143:55-65.

Page, A.E., H.F. Stills, Y. Chander, C.J. Gebhart, and D.W. Horohov. 2011. Adaptation and validation of a bacteria-specific enzyme-linked immunosorbent assay for determination of farm-specific Lawsonia intracellularis seroprevalence in central



Adams, A.A. 2011. Weaning stress and nutritional influences. The Horse, September 14th, http://www.thehorse.com/View-Article.aspx?ID=18822.

Bailey, E. 2011. Screening for foal immunodeficiency syndrome. Veterinary Record 169(25):653-654.

Bailey, E., J.J. Go, and U. Balasuriya. 2011. Association of ECA11 haplotype with susceptibility for in vitro infection with Equine Arteritis Virus. Ninth Dorothy Russell Havemeyer Horse Genome Workshop; Minneapolis, MN; July 27-29, 2011.

Camargo F.C., K.A. Summers, and P.J. Timoney. 2011. Fact Sheet: Equine Viral Arteritis. Cooperative Extension Service ID-197.

Chambers, T.M. 2011. Updating equine influenza. Lloyd’s Equine Disease Quarterly. 20:4.

Non-Refereed Articles

Kentucky Thoroughbreds. Equine Veterinary Journal 43(Supplement 40):25-31.

Perglione, C.O., M. Cordoba, G. Echeverria, P.J. Timoney, S. Tordoya, F. Darqui, G. Metz, S. Mino, L. Becerra, M. Serena, A. Vissani, T. Gonzalez, M. Silvestrini, S. Corva, L. Uncal, J. Dayraut, A. Badaracco, and M. Barrandeguy. 2011. Equine viral arteritis outbreak in Argentina. Proceedings of the 114th Annual Meeting of the USAHA, pp. 320-329.

Pozor, M.A., J. Muehlhaus, A. King, M.L. Macpherson, M.H.T. Troedsson, and C.S. Bailey. 2011. Effect of pentoxifylline treatment on testicular perfusion and semen quality in miniature horse stallions. Theriogenology 76(6):1027-35.

Squires, E.L. and B. Simon. 2011. Safety and efficacy of a controlled release deslorelin acetate product (SucroMateTM) for induction of ovulation in mares. Clinical Theriogenology. 3(3):353.

Sturgill, T.L., D. Strong, C. Rashid, A. Betancourt, and D.W. Horohov. 2011. Effect of Propionibacterium acnes-containing immunostimulant on interferon-gamma (IFNgamma) production in the neonatal foal. Veterinary Immunology and Immu-nopathology 141:124-127.

Summers-Lawyer, A.K., Y.Y. Go, Z. Lu, P.J. Timoney, P.M. McCue, J. Zhang, K.M. Shuck, and J. Bruemmer. 2011. Re-sponse of stallions to primary immunization with a modified live equine viral arteritis vaccine. Journal of Equine Veterinary Science 31:129-138.

Sun, L, A.A. Adams, A.E. Page, A. Betancourt, and D.W. Horohov. 2011. The effect of environment on interferon-gamma production in neonatal foals. Veterinary Immunology & Immunopatholology 143:170-175.

Timoney, J.F., N. Kalimuthusamy, S. Velineni, J.M. Donahue, S.C. Artiushin, and M.A. Fettinger. 2011. A unique geno-type of Leptospira interrogans serovar Pomona type kennewicki is associated with equine abortion. Veterinary Microbiol-ogy 150(3-4):349-353.

Vanderman, K.S., M. Tremblay, W. Zhu, M. Shimojo, M.J. Mienaltowski, S.J. Coleman, and J.N. MacLeod. 2011. Brother of CDO (BOC) expression in articular cartilage. Osteoarthritis and Cartilage 19:435-438.

Waller, A.S., R. Paillot, and J.F. Timoney. 2011. Streptococcus equi: a pathogen restricted to one host. Journal of Medical Microbiology 60(Pt 9):1231-1240.

Woodward, E., M. Christofferson, J. Campos, E.L. Squires, M.H.T. Troedsson. 2011. Susceptibility to delayed uterine clearance after breeding: relationship to endometrial biopsy score and age, and variations between seasons. Clinical Therio-genology. 3(3): 361.

Yeargan, M.R., and D.K. Howe. 2011. Improved detection of equine antibodies against Sarcocystis neurona using polyva-lent ELISAs based on the parasite SnSAG surface antigens. Veterinary Parasitology 176:16-22.


Scientific PublicationsDwyer, R.M. 2011. Commentary. Lloyd’s Equine Disease Quarterly. 20:1.

Dwyer, R.M. 2011. Biosecurity during horse event. Lloyd’s Equine Disease Quarterly 20:3.

Faaberg, K.S., U.B.R. Balasuriya, A.E. Gorbalenya, and E.J. Snijder. 2011. Equine arteritis virus. In: Davison, A.J., et al. (chair), Vetebrate Virus Subcommittee. International Committee on Taxonomy of Viruses (ICTV) Online Database. http://w Chapter 03.004.0.01.001. 2011. ww.ictvdb.org/.

Go, Y.Y., U.B.R. Balasuriya, and E. Bailey. 2011. Genome wide association study for susceptibility of horses for in vitro infection with equine arteritis virus. Journal of Equine Veterinary Science 31(5):244-245.

Issel, C. 2011. New control strategies for equine infectious anemia (Commentary). The Horse (accessed at: http://cs.thehorse.com/blogs/across-the-fence/archive/2011/07/28/new-control-strategies-for-equine-infectious-anemia-commentary.aspx).

Issel, C., and T. Cordes. 2011. Equine Infectious Anemia: How to Avoid Spreading It. The Horse (accessed at: http://www.thehorse.com/18596).

Issel, C. and P.J. Timoney. 2011. The changing face of mosquito-borne diseases: 2010. Equine Disease Quarterly 20(1):4.

Lear, T.L. 2011. Congenital flexural limb deformities in foals. Bluegrass Equine Digest, August, http://www.thehorse.com/ViewArticle.aspx?ID=18727.

Lear, T.L., R.B. McGee, D.A.F. Villagomez, E. Bailey. 2011. Equine disorders of Sexual Development: 17 cases of XX SRY-negative, XY SRY-negative or XY SRY-positive genotypes. International Plant and Animal Genome Conference XIX; W515, P616.

MacLeod, J.N. 2011. Stem cells and regenerative medicine – Pay attention. Equine Disease Quarterly 20(4):1.

Nielsen, M.K. 2011. Horses in the morning. Live appearance on radio show 10/19/11.

Nielsen, M.K. 2011. Safer deworming. Equus 9:48.

Petersen, J.L., J.R. Mickelson, L.S. Ansersson, E. Bailey, et al. 2011. The Equine Diversity Consortium: An International Collaboration to Describe Genetic Variation in Modern Horse Breeds. International Plant and Animal Genome Conference XIX; P617.

Timoney, P.J. 2011. Resurgence of Glanders: A Cause for Increasing International Concern. Proceedings of the 115th An-nual Meeting of the United States Health Association.

Tobin, T., and K. Brewer. 2011. Lasix and bleeders: A classic American horsemens’ story. Winter issue, The Horsemens Journal http://www.hbpa.org/HorsemensJournalDisplay.asp?section=9&key1=13886.


Presentations/Meetings AttendedFaculty at the Gluck Equine Research Center are frequent travelers. They are guest speakers at veterinary confer-ences and meetings locally, nationally and internationally in their respected fields throughout the year.

INTERNATIONALBall, B.A. “Ultrasonographic and endoscopic examinations of stallions’ reproductive organs,” “Endocrinological evalu-ation of prospective and active breeding stallions,” “Diseas-es of the scrotum and testis,” “Testicular biopsy in the stal-lion,” “Sperm transit and storage in the mare reproductive tract,” “Oxidative stress in normal and abnormal function of equine spermatozoa,” and “Sperm motility, morphology and viability: research with aims to improve.” Rossdales Stallion Subfertility Course, Newmarket, UK.

Cook, R.F. “EIAV immunoblot antigen production, stan-dardization and validation,” “Choice and use of recombinant antigen for the development of diagnostic tools for EIAV” and “Overview of EIAV molecular diagnosis and research.” Presented at the request of the Italian Ministry of Agricul-ture during a workshop on equine infectious anemia at the Instituto Zooprofilittico Sperimentale, Della Regioni Lazio e Toscana. Rome, Italy, February

Fulgêncio, J.Q., Y.Y. Go, U.B.R. Balasuriya, F.G. de Olivei-ra, R.E.G. de Moura, J.K.P dos Reis, R.C. Leite. “Serologi-cal evidence of equine arteritis virus infection in equidae of Minas Gerais, Brazil.” XXIIth Virology Brazilian Society (Sociedade Brasileira De Virologica), Atibaia, São Paulo, Brazil. October 23-26

Howe, D.K., S. Dangoudoubiyam, A. Gautam, C.L. Schardl,

J. Jaromczyk, T. bullock, J.C. Kissinger, J. Bridgers, R. Na-masivayam. “A genome project for S. neurona, the cause of equine protozoal myeloencephalitis.” World Association for the Advancement of Veterinary Parasitology Meeting. Bue-nos Aires, Argentina

Donahue J.M., Timoney P.J., Carleton C.L., Marteniuk J.V., Seles S.F. and Meade B.J. “A Study of the Occurrence, Prev-alence and Persistence of Taylorella asinigenitalis in Male Donkeys.” (Presented by C. Carleton). 12th World Equine Veterinary Association Congress. Hyderabad, India, Nov. 2-5

MacLeod, J.N. “Complexity of the Equine mRNA Tran-scriptome.” 4th International Symposium on Animal Func-tional Genomics. Dublin, Ireland

Squires, E.L. Argentina Congress on Equine Reproduction, Mendoza, Argentina. May 4-6

Timoney, P.J. Animal Health and Veterinary Laboratories Agency International Conference on Animal Diseases, Roy-al Holloway, University of London. Surrey, UK

Timoney, P.J. Joint FEI-OIE Meeting on International Movement of Horses. Guadalajara, Mexico

NATIONALAdams A., A. Simpson, D.W. Horohov. “The effect of age on telomerase activity and reactive oxygen species (ROS) production in plasma and peripheral blood mononuclear cells of horses.” 92nd Conference of Research Workers in Animal Disease (CRWAD) Meeting. Chicago, IL, Dec. 4-6

Artiushin, S.C., J.F. Timoney, M. Fettinger, L. Fallon, R. Rathgeber. “Prepartum Immunization of Mares with Bind-ing Domains of Toxin A and B of Clostridium difficile Elicit Antibodies that Block Toxin Binding.” ICAAC Annual Meeting, Chicago, IL. September

Artiushin, S.C., S. Velineni, J.F. Timoney. “Identification of antigens for more reliable Streptococcus equi specific ELISA assay.” 92th Annual Meeting of the Conference of Research Workers in Animal Diseases. Chicago, IL. Decem-ber 3

Artiushin, S.C., J.F. Timoney, L. Fallon, R. Rathberger. “Antitoxic activity of serum and colostrum antibodies in-duced by prepartum immunization of mares with binding domains of toxin A and B of Clostridium difficile.” 4th In-ternational Rushmore Conference on Enteric Diseases. Chi-

cago, IL. December

Ball, B.A. “Embryonic and early pregnancy loss in mares” and “Reproductive Endocrinology of the Mare.” 48th An-nual Conference for Veterinarians, University of Georgia.

Bailey, E. Participate in horse technical committee and executive committee meetings of NRSP8; poster presenta-tions. Plant & Animal Genome XIX Conference, San Diego, CA. January

Bailey, E. Invited Speaker. Havemeyer Equine Ophthalmol-ogy Conference, West Palm Beach, FL. April 1-2

Bailey, E. “Horse Whole-Genome Sequence: Messages Written in DNA.” Department lecture at Washington State University, Pullman, WA. May 16-17

Bailey, E. Larsen Distinguished Speaker Series, “Genome Wide Association Studies (GWAS) for Horse Diseases: Op-portunities and Early Lessons.” Washington State Univer-sity, Pullman, WA. May 17


Bailey, E. Oral presentations. Equine Science Society, Murfeesboro, TN. May 30-June 3

Bailey, E. Poster presentation. (Panel chair, oral presenta-tion). 9th Dorothy Russell Havemeyer Horse Genome Work-shop, Minneapolis, MN. July 27-29

Bailey, E. Organize and Chair session on Horse Genetics. Conference on Equine Health on occasion of the 30th An-niversary of the Dorothy Russell Havemeyer Workshops, Saratoga, NY. Aug. 16-18

Bailey, E. “Genetics and Genomics before and after Have-meyer.” 30th Anniversary Havemeyer Workshop, Saratoga, NY. Aug. 17-20

Balasuriya, U.B.R., A.E. Firth, J.C. Zevenhoven-Dobbe, N.M. Wills, Y.Y. Go, J.F. Atkins, E.J. Snijder, C.C. Post-huma. “Discovery of a novel small arterivirus gene that overlaps the GP5 coding sequence and is important for virus production.” American Society for Virology, Minneapolis, MN. July 16-20

Balasuriya, U.B.R. “Identification of virulence determi-nants of T953 (Findlay) strain of EHV-1: experimental in-oculation of mice and horses with the cell culture adapted strains” and “Molecular Characterization of Field Strains of EHV-1.” Third Havemeyer Equine Herpesvirus-1 Work-shop, Steamboat, CO. Sept. 18-23

Chambers, T.M. “Equine influenza: Progress and prob-lems.” 30th Anniversary Havemeyer Workshop, Saratoga, NY. Aug. 17-20

Dangoudoubiyam, S., D.K. Howe, A. Gautam, C.L. Schardl, J. Jaromczyk, T. Bullock, J.C. Kissinger, J. Bridgers, S. Na-masivayam. “A genome sequencing project for the apicom-plexan parasite Sarcocystis neurona.” Annual Midwestern Conference of Parasitologists, South Bend, IN, June

Dangoudoubiyam, S., D.K. Howe, A. Gautam, C.L. Schardl, J. Jaromczyk, T. Bullock, J.C. Kissinger, J. Bridgers, S. Na-masivayam. “A genome sequencing project for the apicom-plexan parasite Sarcocystis neurona.” American Association of Veterinary Parasitologists Meeting, St. Louis, MO, July

Dunham, J., C. Liu, D.W. Horohov. “Comparison of the ability of two different adjuvants to stimulate antigen pre-senting cells function in vivo.” Conference of Research Workers in Animal Diseases, Chicago, IL. Dec. 3

Dwyer, R.M. Taught “Strengthening Community Agrosecu-rity Planning” at a two-day workshop. Beulah, ND, January 9-13

Dwyer, R.M. St. Kitts for a pre-veterinary advisor tour of Ross University’s School of Veterinary Medicine. North Brunswick, NJ, March 4-9

Dwyer, R.M. FBI International Agroterrorism Conference. Kansas City, KS, April 25-29

Dwyer, R.M. “Biosecurity and Disaster Preparedness” at Louisiana State University College of Veterinary Medicine. “Disaster Preparedness for Equine Professionals.” New Or-leans, LA Society of Animal Science panel discussion. July 12-14

Even, D.L., C.J. Issel, S.J. Cook, D.W. Horohov, R.F. Cook. “Deacylated polyethyleneimine and IL-15 expression con-structs enhance humoral and cellular immune responses to DNA vaccination in horses.” Conference of Research Work-ers in Animal Disease (CRWAD) Meeting. Chicago, IL, Dec. 4-6

Gautam, A.*, S. Dangoudoubiyam, J.P. Dubey, W.J. Saville, and D.K. Howe. “Examination of the surface antigen (Sn-SAG) gene family in Sarcocystis neurona.” (*A. Gautam received the George R. LaRue award for best student presen-tation). Annual Midwestern Conference of Parasitologists. South Bend, IN. June

Gautam, A., S. Dangoudoubiyam, J.P. Dubey, W.J. Saville, and D.K. Howe. “Examination of the surface antigen (Sn-SAG) gene family in Sarcocystis neurona.” American As-sociation of Veterinary Parasitologists Meeting. St. Louis, MO. July.

Go, Y.Y., U.B.R. Balasuriya, E. Bailey. “Genome wide as-sociation study for susceptibility of horses for in vitro in-fection with equine arteritis virus.” Equine Science Society Meeting, Munfreesboro, TN. June 1-3

Go, Y.Y., J.Q. Fulgencio, J.R. Campos, P.J. Heney, R.F. Cook, P.J. Timoney, U.B.R. Balasuriya. “In vitro suscepti-bility or resistance of equine CD3+ T lymphocytes to equine arteritis virus: Is there a correlation with clinical outcome to infection in horses?” The XIIth International Nidovirus Symposium. Traverse City, MI. June 4-9

Go, Y.Y., P.J. Timoney, E.J. Snijder, U.B.R. Balasuriya. “Humoral antibody response to the nonstructural proteins of equine arteritis virus.” The XIIth International Nidovirus Symposium. Traverse City, MI. June 4-9

Go, Y.Y., Y. Li, Z. Chen, D. Yoo, P.J. Timoney, E.J. Snijder, Y. Fang, U.B.R. Balasuriya. “Equine arteritis virus does not induce type I interferon α/β production in equine endothe-lial cells: Identification of nonstructural protein 1 as a main interferon antagonist.” The XIIth International Nidovirus Symposium. Traverse City, MI. June 4-9

Go, Y.Y., D. Cook, P.J. Timoney, E. Bailey, U.B.R. Bala-suriya. “Genome Wide Association Study to identify the genetic determinants of susceptibility of horses of EAV infection.” The XIIth International Nidovirus Symposium. Traverse City, MI. June 4-9

Presentations/Meetings Attended


Go Y.Y., Y. Li, D. Yoo, P.J. Timoney, Y. Fang, U.B.R. Bala-suriya. “Equine arteritis virus does not induce type I inter-feron α/β production in equine endothelial cells.” American Society for Virology, Minneapolis, MN. July 16-20

Gong, Z., L. Sun, D. Horohov. “A specific CpG site demeth-ylation in the IFN-gamma gene promoter region of different aged equine.” Conference of Research Workers in Animal Diseases, Chicago, IL. Dec. 3

Holl, H., T. Lear, R. Nolan-Watson, G. Tyner, S. Brooks. “Detection of Two Equine Trisomies Using SNP-CGH.” 10th Dorothy Russell Havemeyer Foundation International Equine Genome Mapping Workshop. Minneapolis, MN, July

Horohov, D.W. “The effect of age on immune function in horses: impact on vaccination and antimicrobial therapy.” 2011 ELITE Meeting, Chicago, IL. Aug. 16

Horohov, D.W. “Immunoregulation: Models of cytokine-mediated equine immune responses.” Havemeyer Founda-tion, Saratoga Springs, NY, Aug. 17-20

Horohov, D.W. “Alternative measures of cell-mediated im-munity in the horse.” Havemeyer Foundation, Steamboat, CO. Sept. 18-23

Howe, D.K., C.L. Schardl, and J.C. Kissinger. “A genome sequence for the apicomplexan Sarcocystis neurona.” Mi-crobial Genome Sequencing and Microbial Observatories Programs Workshop, Plant and Animal Genomes Confer-ence, San Diego, CA. January

Lear, T.L., T. Raudsepp, J. Lundquist, S. Brown. “A chro-mosome translocation [64,XX,t(2;13)] in a thoroughbred mare with repeated early embryonic loss.” 2011 Equine Sci-ence Society Conference, Murfreesboro, TN. May

Lear, T.L., H. Holl, S. Brooks.“Detection of a 1Mb chromo-some deletion in horses using SNP-CGH: A potential cause of choanal atresia.” 10th Dorothy Russell Havemeyer Foun-dation International Equine Genome Mapping Workshop. Minneapolis, MN, July

Liu, C., S.J. Cook, J.K. Craigo, C.J. Issel, R.C. Montelaro, D.W. Horohov. The maturation of equine infectious anemia virus (EIAV) envelope-specific immune responses in vivo after exposure to a live-attenuated vaccine. Conference of Research Workers in Animal Diseases, Chicago, IL. Dec. 3

MacLeod, J.N. “Equine articular cartilage maturation and repair: new technologies and models to address longstand-ing research questions.” Virginia Polytechnic Institute. Blacksburg, VA

MacLeod, J.N. “Efforts to analyze the mRNA transcrip-tome.” Havemeyer International Equine Genomics Work-

shop. University of Minnesota. St. Paul, MN

MacLeod, J.N. “Profiling gene expression across the ge-nome: The opportunity to find the unexpected.” Havemeyer Foundation 30th Anniversary Workshop, Skidmore College. Saratoga Springs, NY

McGee, R.B., T.L. Lear, K.T. Graves, E. Bailey. “Char-acterization of SRY deletions in female horses with 64,XY karyotype.” XIX Plant and Animal Genome Conference. San Diego, CA. January

Miszczak, F., S. Pronost, U.B.R. Balasuriya, L. Legrand, B. Ferry-Abitbol, C. Christel Marcillaud-Pitel, A. Vabret. “Mo-lecular epidemiology and determination of the source of the 2007 French equine arteritis virus outbreak.” The XIIth In-ternational Nidovirus Symposium. Traverse City, MI. June 4-9

Misczcak, F., J. Dina, S. Corbet, S. Pronost, U.B.R. Balas-uriya, A. Vabret. “First detection of an equine coronavirus in France.” The XIIth International Nidovirus Symposium. Traverse City, MI. June 4-9

Shuck, K.M., F. Miszczak, Z. Lu, Y.Y. Go, J. Zhang, S. Ste-phen A. Vabret, S. Pronost, G. Fortier, P.J. Timoney, U.B.R. Balasuriya. “Evaluation of two magnetic bead-based viral nucleic acid purification kits and three real-time RT-PCR reagent systems in two TaqMan assays compared to virus isolation for equine arteritis virus detection in semen.” The XIIth International Nidovirus Symposium. Traverse City, MI. June 4-9

Squires, E.L. International Embryo Transfer Meeting. Or-lando, FL. Jan. 8-12

Squires, E.L. Southern States Equine Feed Master. Raleigh, NC. Feb. 1-3

Squires, E.L. Equine Breeding and Management and Arti-ficial Insemination Conference. Ohio State University, Co-lumbus, OH. Feb. 5-6

Squires, E.L. American Quarter Horse Association Re-search Committee meeting. Dallas, TX. Feb. 21-22

Squires, E.L. American Quarter Horse Association Re-search Committee meeting and Annual Conference. Dallas, TX. March 4-7

Squires, E.L. Select Breeders meeting. Aubrey, TX. April 25-27

Squires, E.L. Equine Science Society Meeting. Murfrees-boro, TN. May 31-June 4

Squires, E.L. Bioniche Annual Business Meeting. Fort Col-lins, CO. Aug. 1-4


STATEWIDENote: Many of the meetings held in Kentucky were international or national conferences or symposiums.

Adams., A.A. “Getting the right start - The overlooked art of nutritional influences on weaning stress ‘The importance of the immune system.’” 27th Alltech International Feed In-dustry Symposium. Nicholasville.

Artiushin, S.C. and J.F. Timoney. “Isothermal Helicase-Dependent DNA Amplification Assays for Detection of Bac-terial Pathogens of Horses.” 7th KY Innovation and Entre-preneurship Conference. Louisville. May

Graves, K.T. “The Genetic Toolbox: Beyond answering the question ‘Who’s Your (Horse’s) Daddy?’” Friesian Horse Association of North America annual convention. Lexing-ton. March 2

Howe, D.K. “Sarcocystis neurona, the Primary Cause of EPM.” Advances in Equine Neurological Diseases Sympo-sium. Lexington

Bailey, E. University of Kentucky Endowed Chairs and Professor’s First Friday Lecture; Book review of Maryjean Wall’s “How Kentucky Became Southern.” Nov. 4

Bailey, E. “Nature and Extent of Genetic Variation Among Thoroughbred Horses.” Thoroughbred Pedigree, Genetics and Performance Conference. Lexington. Sept. 7-8

MacLeod, J.N. “Genetics 101: Basic Terminology and Con-cepts of Gene Structure and Expression.” 2011 Thorough-bred Pedigree, Genetics, and Performance Conference. Lex-ington

Squires, E.L. American Saddlebred Convention. Lexing-ton. Feb. 18

Squires, E.L. Princeton Field Day. June 20-21

Timoney, P.J. Second Annual Kentucky Breeders’ Short Course. Lexington

Tobin, T. Presentation on certified reference standards syn-thesis. Kentucky Science and Engineering Foundation meet-ing. Louisville. May 26

Squires, E.L. Society for Theriogenology. Milwaukee, WI. Aug. 9-11

Squires, E.L. 30th Anniversary of Havemeyer Symposium. Saratoga, NY. Aug 17-20

Squires, E.L. Havemeyer Symposium on In Vitro Fertiliza-tion, Hilton Head, SC. Nov. 10-12

Squires, E.L. American Association of Equine Practitio-ners, San Antonio, TX. Nov. 17-22

Squires, E.L. Select Breeder Service Annual Meeting. Chesapeake, MD. Dec. 7-9

Sun, L., Z. Gong, D.W. Horohov. “The DNA promoter of the interferon gamma gene (Ifng) is hypermethylated in neonatal foals.” Conference of Research Workers in Animal Diseases, Chicago, IL. Dec. 3

Timoney, J.F. 30th Anniversary Havemeyer Workshop. Saratoga, NY. Aug. 17-20

Timoney, J.F. “Update on strangles pathogenesis and epi-demiology” and “Strangles management and control” Stran-gles Detection and Surveillance Workshop. University of Maine, Orono, ME. Nov. 5

Timoney., P.J. NIAA Annual Conference, Equine Commit-tee. San Antonio, TX

Timoney., P.J. USDA Foreign Animal and Emerging Dis-eases Course, University of Tennessee. Knoxville, TN

Timoney., P.J. 30th Anniversary Havemeyer Workshop. (Infectious Diseases Session Chair) Saratoga, NY. Aug. 17-20

Timoney., P.J. 115th Annual Meeting of the United States Animal Health Association. Buffalo, NY

Tobin, T. “The McKinsey Report 20 years later.” National Horsemen’s Benevolent and Protective Association. Hot Springs, AR, March 19

Tobin, T. National Lasix Summit organized by the Racing medication and Testing Consortium (RMTC), the American Association of Equine Practitioners (AAEP), and the Na-tional Thoroughbred Racing Association (NTRA). Belmont Park, NY. June

Tobin, T. Presented paper on therapeutic efficacy of furose-mide. National Horsemen’s Benevolent and Protective As-sociation, Seattle, WA. July

Zoll, M.M., J. Dunham, A. Betancourt, A. Page, S. Reedy, T. Chambers, D. Horohov. “The effect of flunixin meglumine on the equine immune response to vaccination.” Conference of Research Workers in Animal Diseases, Chicago, IL. Dec. 3


PlatinumAmerican Endowment FoundationBlood Horse PublicationsDarley Stud Management, LLCFlorida Horsemen’s Charitable FoundationGeoffrey C. Hughes Foundation, Inc.Horseman’s Financial Group, Inc.Indiana Horsemen’s Benevolent & Protective AssociationLloyd’s Underwriter FundsLourie Foundation Mt. Brilliant Family FoundationPennsylvania Horsemen’s Benevolent & Protective Association

GoldAshford Stud/Creek View FarmDr. Stuart E. Brown IIChemSolutions, LLCDarley Stud Management, LLCAdele B. DilschneiderFasig-Tipton Company, Inc.Dr. William D. Fishback Jr.Horsemen’s Benevolent & Protective AssociationLouise Ireland HumphreyDr. Charles J. and Patricia S. Issel Kentucky Association of Equine PractitionersKentucky Horsemen’s Benevolent & Protective AssociationMisdee Wrigley and James Mather Miller Charitable FoundationBetty W. MullenDr. Janice Spencer Murray and Mac MurrayThe National H.B.P.A., Inc.Nebraska Horsemen’s Benevolent & Protective AssociationThe Oxley FoundationReproductive ManagementDr. W. Thomas RiddleShawnee FarmStonereath Farms, Inc.Thoroughbred Racing Association

SilverAlex Boone Charitable TrustClaiborne FarmsClarkland FarmsKyle W. CooperDenali StudEast Tennessee Clinical Research, Inc.Equine Medical AssociatesKeeneland AssociationJP Morgan Chase Bank N.A.The Roemer FoundationDr. Edward L. SquiresT and D Concrete, Inc.Dr. Mats H. TroedssonDr. Patricia L. Ziefle

BronzeElizabeth A. BarryKatrina H. BeckerL.A. BrownPatrick G. DooherDr. Claire Latimer EmbertsonDr. Rolf M. EmbertsonDarrilyn G. FraserDr. Lynette D. GreenwoodFrances M. HartwellMartha K. HoffVirginia HowardStanley H. JonesMary Ann JubinDonna J. LassanskeDr. Roger A. MagnussonRonald McAnally Inc. Circee McDonaldJudy P. MillerMott Thoroughbred Sales, Inc.Joe MulhollandBetty W. MullenThomas E. ShartleJohnny M. SmithArnold K. SmolenSugar Hill FarmsSummerhill FarmAnne F. ThoringtonJ. David Whitehouse

Thank you to our 2011 donors who generously supported the Gluck Center’s mission in improving the health and well-being of the horse.

2011 Donors: Gluck Equine Research Center


research report 2011 - gluck.ca.uky.edu

Documents