s1. mcf7 m (high density) day 3 day 5 s2 ab s3 s4 networkspvaluegenes from active data...
TRANSCRIPT
S1
MCF7M (high density)
Day 3 Day 5
S2
A B
S3
S4Networks pValue Genes from Active Data
Chemotaxis 7.991E-11 IL-1 beta, CCR2, GRO-3, Lck, CCL13, Galpha(q)-specific peptide GPCRs, Galpha(i)-specific peptide GPCRs, SDF-1, IL-16, CCL7, GRO-2, PD-ECGF (TdRPase), GCP2
Proteolysis_ECM remodeling 1.820E-09 MMP-13, MMP-14, TIMP3, MMP-1, TIMP4, MMP-15, MMP-24, MMP-1, MMP-25, Stromelysin-1
Inflammation_Jak-STAT pathway
4.161E-09 IL21R, CCR2, PDGF-R-beta, IL5RA, CCL13, PDGF receptor, IL-2R gamma chain, IL-21 receptor, SDF-1, IL10RA, IGF-1, CCL14, IL-20
Proteolysis_Connective tissue degradation
4.951E-08 MMP-13, MMP-14, TIMP3, MMP-19, TIMP4, MMP-15, MMP-24, MMP-1, MMP-25, Stromelysin-1
Cell adhesion_leukocyte chemotaxis
9.863E-07 CCR2, GRO-3, Lck, CCL13, Galpha(q)-specific peptide GPCRs, Galpha(i)-specific peptide GPCRs, SDF-1, CCL14, CCL7, GRO-2, GCP2
Immune response_Th17-derived cytokines
1.520E-06 IL-1 beta, MMP-13, SDF-1, CCL7, RANKL(TNFSF11), GCP2, MMP-1, Stromelysin-1
Development_Blood vessel morphogenesis
2.810E-06 IL-1beta, FGF8, Galpha(q)-specific peptide GPCRs, Galpha(i)-specific peptide GPCRs, MMP-19, SDF-1, ALK-2, VEGFR-2, TIE, PD-ECGF (TdRPase), VEGF-D
Cell adhesion_Platelet-endothelium-leukocyte interactions
1.497E-05 IL-1 beta, MMP-13, PDGF-R-beta, Endoglin, PDGF receptor, VEGFR-2, Thrombospondin 4, TGF-beta receptor type II, PDGF-R-alpha
Apoptosis_Anti-Apoptosis mediated by external signals via PI3K/AKT
2.345E-05 IL-1 beta, FGF8, PDGF-R-beta, PDGF receptor, GFRalpha1, IGF-1, VEGFR-2, NT-3, VEGF-D, PDGF-R-alpha
S5
Dataset Number of patients
Number in patient subset
Comparison
Scheutz et al 14 14 DCIS vs IDC
Ma et al (Ref 32)
66 20 Epithelium vs Stroma
Boersma et al 95 83 Epithelium vs Stroma
S6
S7
1750 1500 1250 1000
0.0
0.2
0.4
0.6
0.8
1.0
1.2
3000 2950 2900 2850 28000.0
0.2
0.4
0.6
0.8
1.0
3500 3000 2500 2000 1500 1000
0.0
0.2
0.4
0.6
0.8
1.0
1.2
MCF-7M (high) + E2
MCF-7M (high) + Veh
MCF-7M (low) + E2
MCF-7M (low) + Veh MCF-7 + Veh MCF-7 + E2
Abs
orba
nce
Wavenumber (cm-1)
S8
1700 1500 1300 1100
0.0
0.2
0.4
0.6
0.8
1.0
1.2
3000 2950 2900 2850 2800
0.0
0.2
0.4
0.6
0.8
1.0
3500 3000 2500 2000 1500 1000
0.0
0.2
0.4
0.6
0.8
1.0
1.2
MCF-7+ Veh MCF-7 + E2
MCF-7+CM + Veh MCF-7+ CM E2
Abs
orba
nce
Wavenumber (cm-1)
S9MCF-7 MCF-7 + HMF