SCIENCE HAS

FOUND A HOME

AT MONAVIE

Dr. Alex Schauss

MonaVie 2005

SAB Formed 2008

• Dr. Alex Schauss

• Dr. Penny Kris-Etherton

• Dr. Nathan Wong

• Dr. Stephen Talcott

• Dr. Bernie Landes

SAB Expands 2010

• Dr. Paul Clayton

MonaVie’s Commitment

to Science

• Demonstrated commitment to high quality research

• Basic and clinical studies:

MonaVie Pulse® (cardiovascular health)

MonaVie Active® (antioxidant protection/joint mobility)

MonaVie Emv® (energy/cognition)

MonaVie (M)mūn® (immune function)

MonaVie RVL™ (weight management)

• A company respected by the scientific community

• Over 60 funded studies — 15 published in peer reviewed

journals

Basic Research

• Understanding The

unique health benefits of

Açai and other

nutraceuticals used in

MonaVie® Products

THE YEAR

OF SCIENTIFIC

DISCOVERIES

False Claims on Maqui Berry’s

ORAC Value

• Maqui berry’s ORAC score: Several companies have

claimed that the is significantly higher than açai’s.

• Turns out the claim is false! The lab did not perform the

test.

• Lab responded:

• ―We tried to reach ―company‖… to remove the false

information from their website and materials…this kind of

misinformation is frustrating.‖

• ―Spoke to company. They will remove it.‖

Published Açai Research

in PubMed1994-2005

• Up to 2003: 0 citations

• 2004: 2 citations

• 2005: 4 citations

• 2006: 7 citations

• 2007: 9 citations

• 2011: 79 citations

• 2006: Schauss AG, et al. Federation of American dSocieties in Experimental Biology Journal 20: A145.

• 2006: Schauss AG, et al. Journal of Agricultural and Food Chemistry 54: 8598-8603.

• 2006: Schauss AG, et al. Journal of Agricultural and Food Chemistry 54: 8604-8610.

• 2006: Schauss AG. Acai (Euterpe oleracea): An Extraordinary Antioxidant-Rich Palm Fruit.

• 2007: Schauss AG, et al. Proceedings of the Second International Symposium on Human Health Effects of Fruits and Vegetables, Houston, Texas, pp.

39-40.

• 2007: Schauss AG. Acai (Euterpe oleracea): An Extraordinary Antioxidant-Rich Palm Fruit. First foreign translation of English edition.

• 2008: Jensen GS, et al. Journal of Agricultural and Food Chemistry 56: 8326-8333.

• 2008: Honzel D, et al. Journal of Agricultural and Food Chemistry 56: 8319-8325.

• 2008: Schauss AG. Symposium on Flavor and Health Effects of Small Fruits, Cornucopia, AGFD 95.

• 2008: Schauss AG. Acai (Euterpe oleracea): An Extraordinary Antioxidant-Rich Palm Fruit. Second Edition Second foreign translation of English edition.

• 2009: Schauss AG, et al. Proceedings of the Second International Symposium on Human Health Effects of Fruits & Vegetables. Acta Horticulturae 841:

97-100.

• 2009: Jensen GS, et al. Alternative Therapies in Health and Medicine, 15: S90-S91.

• 2010: Schauss AG. Bioactive Foods in Promoting Health: Fruits and Vegetables. Academic Press: Oxford, pp. 479-490.

• 2010: Schauss AG, et al. Flavor and Health Benefits of Small Fruit. American Chemical Society Symposium Series: Oxford University Press, pp. 213-

223.

2006-2010: Publications on Açai

Research and MonaVie Stream into the

Scientific Literature

A Year of Discoveries on Açai

& MonaVie 2010-2011 • 2010: Sun X, et al. Experimental Gerontology 45: 243-251.

• 2010: Kang J, et al. Food Chemistry 122: 610-617.

• 2010: Schauss AG, et al. Toxicology 278: 39-45.

• 2011: Chenghui X, et al. Atherosclerosis 216: 327-333.

• 2011: Kang J, et al. Food Chemistry 128: 152-157.

• 2011: Jensen GS, et al. Journal of Medicinal Food, Epub ahead of print.

• 2011: Chenghui X, et al. Journal of Nutritional Biochemistry, under

review.

• 2011: Kang J, et al. Submitted for publication.

• 2011: Poulose SM, et al. Submitted for publication.

• 2011: Schauss AG. Submitted for publication.

2006-2011 Presentations at

Scientific Conferences• 2006: Schauss AG, et al. Federation of American Societies in Experimental Biology, San

Francisco, CA. FASEB Journal 20: A145.

• 2007: Schauss AG, et al. Proceedings of the Second International Symposium on Human

Health Effects of Fruits and Vegetables, Houston, Texas.

• 2008: Schauss AG. 236th American Chemical Society National Meeting, Division of

Agricultural and Food Chemistry Symposium on Flavor and Health Effects of Small

Fruits, Philadelphia, PA.

• 2009: Schauss AG, et al. North American Research Conference on Complementary &

Integrative Medicine, Minneapolis, MN.

• 2010: Schauss AG, et al. Annual Meeting of the Society of Toxicology, Salt Lake City, UT.

• 2010: Kang J, et al. Federation of American Societies in Experimental Biology, Anaheim, CA.

• 2011: Xie C, et al. Federation of American Societies in Experimental

Biology, Washington, DC.

• 2011: Kang J, et al. Federation of American Societies in Experimental

Biology, Washington, DC.

Discovery

• Safety of MonaVie® juice studied.

• 13 experimental in vitro and in

vivo studies carried out over two

years.

• No evidence of genotoxicity

or toxicity.

• The no observable adverse effect

level (NOAEL) for juice

established.

Discovery

• MonaVie® juice given to 48 to

84 year olds for 12 weeks.

• Increased serum

antioxidant status

• Improved range of motion

lower back and both knees

• Improvement in activities

of daily living

Discovery

• Açai pulp contains potent

anti-inflammatory and

antioxidant compounds.

• A flavonoid was isolated and

shown to be a potent

compound to help balance

inflammatory mediators.

Brain Health and Aging

• As we age, there is a decline in how efficiently our brain

removes toxic proteins and other debris.

• This is function is called autophagy, or simply brain

housekeeping.

• A decline in autophagy is linked to age-related memory

loss, and mental decline.

• As reported in August 2010, in an animal model, it was

discovered that compounds in açai pulp activate autophagy

and remove toxic proteins.

Poulos, et al. Annual Meeting, Am Chem Soc., August, 2010, Boston. Shukitt-Hale, et

al. Annual Meeting, Society Neuroscience, November, 2010.

Heart Health Myths

1. It only affects the elderly.

2. It doesn’t affect children.

3. It doesn’t affect those who are fit and strong.

4. If I had it, I’d feel sick.

5. If my parents didn’t have it, I won’t get it.

DiscoveryAdding MonaVie juice to the

diet in animal model*:

• Significantly inhibiting formation of

atherosclerotic lesions by nearly 60%

(p<0.001)

Proposed mechanisms of

action:

• Reduced lipid peroxidation

• Increased antioxidant enzymes

• Inhibition of pro-inflammatory

cytokine production

• Chinese (Traditional)

• French

• German

• Korean

• Polish

• Malaysian

• Thai

New 3rd Edition of Açai Book

translated into:

SCIENCE

IN SUPPORT

OF SALES

Applied Research

• Confirming the

benefits of MonaVie®

products as they are

consumed

Essential

Antioxidant Capacity

• Increase in serum

antioxidants

• Inhibition of lipid

peroxidation

MonaVie Active

MonaVieRVL™ Study Results

Conclusions:

•The study* demonstrated

a statistically significant

improvement in body

mass index, weight and

waist circumference.

* Small pilot study with 36 people for 28 days, in

persons 100% compliant with the RVL weight

management program.

Study Results

Study Results

• No increase in heart rate versus placebo

• Perceived exertion lower with Emv

• Reaction time improved with Emv

• Participants felt more vigorous with Emv

• Significant improvement of 20% in short-

term memory with Emv

Emv® Study Results

Acknowledgements:

The author would like to acknowledge

MonaVie for their support of this study.

Robert Girandola, Ed.D.

University of Southern California, Los Angeles, Ca.

Testing the Efficacy of a New Energy Drink

INTRODUCTION METHODOLOGY

CONCLUSIONS

RESULTS

PURPOSEThe present study was designed to determine if a new energy drink would improve

exercise and cognitive functions in human subjects,

when compared to a placebo.

RESULTS: Means, SD, and T-Test Results for Each Measure Following Exercise & Cognitive TestingThe average age of the subjects was 20 yrs. Of the 25, 11 were

females and 14 were males. Average BMI was 22.39 kg/m2 . Heart

rates for the two drinks were 150.52 and 150.16

bpm, respectively, for both days. The HR was an indication that the

exercise intensity was approximately 65-70% of max. The RPE for

the MV drink was 3.90 vs. 4.16 for the placebo condition, a

difference of 6.7%, but this was not statistically significant.

Similarly, the RT/MT values were faster and the POMS vigor score

higher for the MV condition, but neither achieved statistical

significance. Interestingly, the only variable that was statistically

significant was the memory score, 6.00 vs. 5.04 for the placebo

condition.

The popularity of energy drinks is undeniable. Over the past several years a number of energy

drinks and energy shots have been introduced with incredible popularity. The present market

for energy drinks in the USA is in the multi billion-dollar range. Research as to the efficacy of

energy drinks has focused on both cognitive abilities as well as performance variables. Several

recent studies have found that subjects had more alertness and performed better in physical

tasks, following ingestion of an energy drink. However, others have not found similar

improvements.

Since caffeine is the major active ingredient in these energy drinks it is unclear whether the

improvement is related to this substance or other ingredients. In addition, since the caffeine load

can be substantial, the safety of these energy drinks has been called into question. The

introduction of a new energy drink based upon fruit juices and less caffeine was the stimulus

resulting in the current research study.

MonaVie has developed such an energy drink made up of 80% fruit juices. In addition the

carbohydrate source was Palatinose, a slower digested and metabolized sugar. The 8.4 oz can

contains 40 g of CHO (22 g sugar and 15 g Palatinose) 160 Calories. Included in the ingredients

are: caffeine ( < 60 mg), D-ribose, ginseng, green tea and guarana.

A total of 25 volunteer subjects from the University of Southern California were used as subjects. After completion of signed informed consent forms (IRB

approved), they were informed that the study was actually testing two different energy drinks, to determine which one was superior. Prior to reporting to

the laboratory, subjects were given two 9 oz clear plastic, generic bottles of the MonaVie (MV) OR Placebo (P: carbonated Arrowhead water into which

was mixed a red food color dye and half a package of the dietary sweetener, Splenda). The outside of the bottles was marked “A” (MV) or “B”

(Placebo). Subjects were told to take both bottles home and to consume the contents on ONE of the bottles, one hour prior to reporting to the lab.

Subjects were told not to consume and food or beverages after consuming the experimental fluid, prior to reporting to the laboratory. The subjects diet

was not monitored but they were encouraged to consume the same diet on the two testing days. We endeavored to test subjects at the same times on the

two testing days.

Upon reporting to the laboratory subjects were given a choice reaction/movement time test as a warm-up/practice. A Polar heart rate (HR) recording

strap was attached to the chest and then subjects were asked to walk on the treadmill. Prior to the walk, subjects were shown a chart displaying 8 random

words and were told to memorize as many as they could. For the first 2 minutes speed was 3 mph and 0%grade. At the 3rd minute, grade was increased

to 10% and HR was recorded continuously. At the 9:30 minute subject was shown the Borg scale (Rating of Perceived Exertion [RPE] – 0-10) and

asked to rate his/her level of intensity. Following the exercise, subjects were given the RT/MT test, with the average of 5 trials recorded. After the

subjects toweled off and were seated, they were given a piece of paper and were told to write down as many of the 8 words they could remember.

Subjects were then administered the Profile of Mood States (POM) to identify the vigor score. This psychological test has been shown to be a reliable

measure of vigor in both average people and athletes. At the conclusion of the POMS test, subjects were told to write down their subjective feelings after

ingesting the fluid and after the laboratory exercises.

Statistical Analysis: a paired, 2-tailed, student's T-Test was used to compare the means for each measurement between trials (A: MV) and (B: Placebo).

Statistical significance was determined at p<0.05.

We attempted to use both physical and psychological tests to

determine if this new drink improved exercise or cognitive function,

however, based on the data, we were unable to reach any real

conclusions.

With some of the safety concerns regarding current energy drinks

on the market, especially with the large concentration of caffeine in

a relatively small volume, the MV beverage might offer a safer and

healthier alternative. Future research on this energy drink should

focus on a somewhat longer time frame between ingestion and

testing (2 hours) and a more intense and longer bout of exercise.

References: Van den Eynde R, van Baelen PC, Portzky M, Audenaert K. The effects of energy drinks on cognitive performance. Tijdschr Psychiatr. 2008:50 (5): 273-81. Alford C, Cox H, Wescott R. The effects of red bull energy drink on human performance and mood. Amino Acids. 2001;

21(2): 139-50. Rahnama N, Gaeini AA, Kazemi F. The effectiveness of two energy drinks on selected indices of maximal cardiorespiratory fitness and blood lactate levels in male athletes. J Res Med Sci. 2010 May; 15(3): 127-32. Astorino TA, Matera AJ, Basinger J, Evans M, Schurman

T, Marquez R. Effects of red bull energy drink on repeated sprint performance in women athletes. Amino Acids. 2011, Apr 3. Higgins JP, Tuttle TD, Higgins CL. Energy beverages: content and safety. Mayo Clin Proc. 2010 Nov;85(11): 1033-41. Duchan E, Patel ND, Feucht C. Energy drinks:

a review of use and safety for athletes. Phys Sportsmed. 2010 Jun;38(2): 171-9. Ballard SL, Wellborn-Kim JJ, Clauson KA. Effects of commercial energy drink consumption on athletic performance and body composition. Phys Sportsmed. 2010 Apr;38(1): 107-17. Candow DG, Kleisinger

AK, Grenier S, Dorsch KD. Effect of sugar-free Red Bull energy drink on high-intensity run time-to-exhaustion in young adults. J Strength Cond Res. 2009 Jul;23(4): 1271-5. Ivy JL, Kammer L, Ding Z, Wang B, Bernard JR, Liao YH, Hwang Improved cycling time-trial performance after

ingestion of a caffeine energy drink. Int J Sport Nutr Exerc Metab. 2009 Feb; 19(1):61-78.

Studies in Progress

• MonaVie (M)mun® —

confirming immune

support benefits

• 2nd MonaVie RVL

Clinical Study

PRODUCTS

WITH PURPOSE

MonaVie Products...

Best in Class

• MonaVie is a cutting edge nutrition

company providing innovative, science-

based products:

• Functional juices of superior

nutrient density and diversity

• Healthier energy drinks

• Scientifically supported weight

management system

• Searching the world for new ingredients

• Enhance current health benefits

• Create new categories of benefit

• Continuous innovation in

product form, function,

and taste

What Does the Future Hold?