SCIENTIFIXCOVID-19 Related Products

Viral Nucleic Acid Extraction Kits

MACHEREY-NAGEL purification products contribute to the fight against SARS-CoV-2 pandemic.Facing the current COVID19 outbreak molecular testing laboratories rely on workflows to isolate SARS-CoV-2 RNA from respiratory samples.

Multiple labs have included MACHEREY-NAGEL’s NucleoSpin and NucleoMag kits for isolation of viral RNA into their workflows, among them the two WHO reference laboratories, the Institute Pasteur Paris (France) and the National Institute of Health (Thailand).

We are supporting all labs by providing guidelines for sample preparation and nucleic acid extraction with MN kits in compliance with WHO and CDC recommendations. The recommendations include all our NucleoSpin and NucleoMag kits suitable for extraction of viral RNA.

Purification of human or animal viral RNA and DNA from serum, plasma, swab and tissues homegenates. Highly sensitive for DNA and RNA viruses e.g. HIV, HPV, H5N1, HBV, etc.

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Isolation of viral RNA and DNA from cell-free biological fluids in either an 8-well strip or 96-well format.

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Magnetic bead based isolation of viral RNA and DNA from cell-free biological fluids. This kit can be adapted for automation systems.

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NucleoSpin Virus NucleoSpin 8 / 96 Virus NucleoMag Virus

Protocol: Recommendations for respiratory samples (swabs, sputum, bronchoalveolar lavage).

Current WHO References:• Institute Pasteur Paris (Paris, France); NucleoSpin Dx Virus• National Institute of Health (Nonthaburi, Thailand); NucleoSpin RNA Virus

Real-time qPCR for COVID-19 Research

Takara Bio’s One-Step RT-PCR kits were used to screen coronavirus during the initial outbreak in ChinaThe recent emergence of the coronavirus SARS-CoV-2 (the virus that causes COVID-19) has led to a need for rapid and reliable detection. Takara Bio offers solutions to support researchers and clinicians studying this novel coronavirus, such as NGS kits and RT-qPCR kits that can be used with established primers for sensitive detection of SARS-CoV-2 down to five viral copies.

One-step, real-time RT-PCR on purified RNA samples. Amplified products monitored in real time without need for gel electrophoresis after PCR. Validated on COVID-19 screening.

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Application:CDI Enhanced COVID-19 Test EUA

One-step RT-PCR on crude RNA extracts. Highly resistant to PCR inhibitors, e.g. heparin. Suitable for complex RNA templates.

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One-step RT-PCR directly on biological samples, no RNA purification required. Perform one-step RT-PCR detection directly from whole blood, mouth and nasal swabs, and saliva samples.

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Application:Ipsum Diagnostics COVID-19 IDx Assay EUA Summary

One Step PrimeScript RT-PCR Kit(Perfect Real Time)

One Step PrimeScript III RT-PCR PrimeDirect Probe RT-qPCR Mix

See Blogs: • Screening for coronavirus with one-step RT-qPCR: getting ahead of the outbreak• Takara Bio plays a crucial role in fighting coronavirus

Tech Note: Faster, simpler virus detection directly from biological samples

Publication: Comparison of SARS-CoV2 N gene real-time RT-PCR targets and commercially available mastermixesTakara One Step PrimeScript™ III RT-PCR Kit mastermix has improved performance at the lower limit of detection compared to the other mastermixes tested in this study

Isolation of only viral RNA from serum, plasma or cell-free biological fluids. Suitable for downstream RT-PCR and enzymatic reactions. Proven for large variety of viruses e.g. influenza, HCV, HIV, Bird Flu Virus.

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Magnetic bead based isolation of viral RNA/DNA and microbial DNA from whole blood, serum or plasma, feces, tissue or swabs. Can be adapted for manual or automated processing.

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Magnetic-bead purification of viral nucleic acids and bacterial DNA from veterinary and human samples, e.g. whole blood, serum, plasma, tissue homogenates, ear notches and swabs. Process manually or via automated systems (e.g. KingFisher FLEX instrument).

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NucleoSpin RNA Virus NucleoMag Pathogen NucleoMag VET

Next-generation Sequencing for COVID-19 Research

Monitoring the environment for presence of harmful pathogensWe offer a variety of RNA-sequencing kits for unmatched sensitivity and reproducibility for all of your demanding sequencing applications, including COVID-19 research. Our SMARTer RNA-seq products offer sensitivity to detect trace amounts of RNA, and through randomly primed reverse transcription also enable detection of RNA that is in various stages of degradation. In particular, the SMARTer Pico v2 RNA-seq and SMART-Seq Stranded kits work from inputs of <1 ng total RNA to provide comprehensive RNA transcript profiling.

Efficient preparation of Illumina sequencing libraries from picogram input amounts (250 pg–10 ng) of total RNA. Suitable for processing RNA of any quality including FFPE, LCM samples and cell-free RNA.

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Generate cDNA libraries from ultra-low amounts of total RNA, or directly from 1–1,000 intact cells (or 10 pg–10 ng of total RNA).

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Generate strand-specific RNA-seq libraries for Illumina® sequencing from 1–1,000 sorted cells or 10 pg–10 ng of purified total RNA. Suitable for whole transcriptome sequencing with strand-of-origin information.

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Generate full-length cDNA directly from single cells. Kit has been validated with 2 pg of total RNA input and with single cells known for low RNA content. e.g. peripheral blood mononuclear cells.

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SMARTer Stranded Total RNA-seq Kit v2 - Pico Input Mammalian

SMART-seq v4 Ultra Low Input RNA Kit - Generate cDNA for mRNA-seq

SMART-seq Single Cell Kit - Generate cDNA for mRNA-seq

SMART-seq Stranded Kit

Understanding the effects of viral infectionOur tools, such as the SMART-Seq v4 and SMART-Seq Single Cell kits, can help researchers understand the expression changes that occur in a host as a result of viral infection and treatment—at both the bulk and single-cell level, respectively—to aid in the development of appropriate treatment. Additionally, both of these kits sequence full-length transcripts enabling cell-type identification as well as providing detailed information on isoform changes, SNPs, or splice variants that can help us better understand the response to the infection and treatments alike.

See Blog: Complete, sensitive, and fast viral genomics with SMARTer Stranded Kit

Tech Note: Unprecedented sensitivity with the SMART-Seq Single Cell Kit

Immune Profiling

CRISPR Screening

Monitoring the immune response to viral infectionTo understand host response when a virus enters the body and later when a treatment is administered, it is important to understand how the transcriptional profiling of a host cell changes. For SARS-CoV-2, in particular, it will be important to see how the B cells and T cells are affected to understand what is going on with the immune system. Our human BCR and TCR kits enable you to answer this and other questions and help ensure we can respond properly now and are prepared for the future.

We also have kits for immune profiling in mice.

Discover host factors critical for SARS-CoV-2 pathogenesis and identify novel therapeutic targetsCRISPR screens in the field of virology are ideally suited for analysing virus-host interactions; determining host factors critical for virus entry, replication, and spread; and identifying and prioritising novel drug targets. Performing a genome-scale CRISPR/Cas9 knockout screen with an sgRNA library can help identify important host factors responsible for disease response.

Generate libraries from 10 ng–10 µg of total RNA from peripheral blood mononuclear cells (PBMCs) or 1–100 ng of total RNA from B cells. Kit can be used to generate data for both heavy and light chains of human IgG and IgM.

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Generate libraries from 10 ng–3 µg of total RNA from peripheral blood or 50–10,000 purified T cells. Kit can be used to generate data for both alpha and beta-chain diversity.

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SMARTer Human BCR Profiling Kit SMARTer Human TCR Profiling Kit

Perform lentiviral-based, genome-wide CRISPR/Cas9 knockout screens in human cells.

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Following screening, NGS analysis can be performed to identify candidate genes of interest.

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Guide-it CRISPR Genome-wide sgRNA Library System Guide-it CRISPR Genome-wide sgRNA Library NGS Analysis Kit

Vaccine Development:

Promising vaccine candidates are further optimised by analysing and comparing B-cell antibody and T-cell responses in vaccinated animals prior to and upon exposure to virus

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The isolation of high quality nucleic acids is the basis for successful downstream applications like genotyping, next generation sequencing, real-time PCR analysis, and other molecular biology techniques. Macherey-Nagel has partnered with several platform providers to co-develop and optimise extraction protocols for various sample materials and target molecules. The combination of MN extraction kits and liquid handling platforms enables isolation of high quality biomolecules (DNA / RNA / protein) suitable for common downstream applications.

View the platform providers we support

Takara Bio COVID-19 Research Summary

HTP Automation Extraction Partners

COVID-19 Multiplex Immunoassays

Takara Bio offers solutions to support researchers and clinicians studying the novel coronavirus, with a shared goal of understanding, treating, and preventing COVID-19 and related diseases. You can depend on our widely used tools that enable accurate detection, purification, sequencing, cloning, genome editing, and immune profiling for your critical work.

We are always here to help you move your research forward. We pledge to provide our customers with first-rate products and services plus expert guidance, so you can focus on the advancement of scientific knowledge and the improvement of human health. Please contact us with any questions, and we will help get you what you need, including bulk, custom, and OEM requests.

Scientifix partners with ELISAGenie, experts in ELISAs, immunoassays and antibodies.

Cytokine Release Syndrome (CRS) is a systemic inflammatory response characterised by the mass release of pro-inflammatory cytokines from immune cells. ELISAGenie has developed Mutiplex Immunoassays to study CRS in COVID-19. Immunoassay kits enables simultaneous and quantitative detection of relevant analytes on any flow cytometer and from a wide variety of samples.

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See Blog: Cytokine Release Syndrome in COVID-19

Contact Scientifix for all your liquid handling automation needs

Automated COVID-19 Testing Workflow

Complete, automated SARS-CoV-2 PCR detection workflowsThe recent outbreak of coronavirus (SARS-CoV-2) has highlighted the need for technologies to support rapid, high-throughput testing of biological samples. PerkinElmer enables labs to maintain sample integrity and avoid cross contamination of SARS-CoV-2 PCR samples by providing validated, modular and scalable, automated workflow solutions. These solutions provide high quality nucleic acid extraction, automation with flexible throughput, and full traceability.

Protect Your Workforce by Minimizing the Risk of Laboratory-acquired COVID-19 Infection During High Volume ProcessingLaboratories need to take appropriate measures to contain potentially infectious materials and prevent secondary infections. The first step of a PCR-based virus detection workflow, nucleic acid isolation, has the highest risk of exposing laboratory employees to pathogens. Reducing manual contact for this step is essential to reduce the risk of laboratory-acquired infection during processing.

PCR Assays for Coronavirus (SARS-CoV-2) DetectionThe following workflow can be used with the PerkinElmer® New Coronavirus Nucleic Acid Detection kit, or any other 2019-nCoV PCR assay.

Technique: Real-time RT-PCRTest Gene Region: ORF1ab & NSample Type: Naso- and Oropharyngeal swabs, BAL, sputum, plasma and serumSample Volume: Up to 400 µLLimit of Detection: 20 copies / mL

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Traceable and reproducible pre-real time PCR sample preparation for high throughput SARS-CoV-2 detection using validated and approved protocols. This automated workstation offers full walk-away pre-PCR sample prep while reducing the risk of hands on errors in HTP SARS-CoV-2 testing.

Shipped with validated sample prep protocols for PerkinElmer SARS-CoV-2 detection PCR kit and can be configured to automate an SARS-CoV-2 assay.

Nucleic acid quality is of major importance when conducting molecular analysis since factors causing degradation and the presence of impurities and enzymatic inhibitors can have a significant impact on the quality of data.

PerkElmer’s chemagen technology address these challenges while offering high recovery of clean nucleic acids suitable for demanding downstream applications.

The chemagic Viral DNA/RNA 300 Kit and the chemagic 360 instrument is recommended for the isolation of nucleic acids for use with the Twist SARS-CoV-2 Research Panels.

Input: up to 400 µLFormat: 96-well plate (70 min / 1-96 samples)Number of Preps: 960Sample Types: Naso- and Oropharyngeal swabs, BAL, sputum, plasma and serum

PerkinElmer New Coronavirus Nucleic Acid Detection Kit

JANUS® G3 PCR Workstationchemagic™ 360 Instrument

Nucleic Acid Extraction Sample Prep / PCR Set Up RT-PCR

Publication: Efficient Extraction of Viral DNA and RNA by the Chemagic Viral DNA/RNA Kit Allows Sensitive Detection of Cytomegalovirus, Hepatitis B Virus and Hepatitis G Virus by PCR

Forehead thermometer. CE certificate.

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500 ml pump bottle. Contains 70% ethanol, kills 99.9% of germs.

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Spray on all surfaces and let settle. Protection against bacteria, fungi and enveloped viruses.

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Infrared Thermometer Hand Sanitisers

Spray Disinfectant, Biocidal-ZF

COVID-19 Detection

Detection of small biological molecules using labeled antibodies and antigens in serum, plasma and whole blood. Hands-free processing of samples, up to 48 samples to be processed per hour with 15-min assays. Use in conjunction with Anti-SARS-CoV-2 IgG/IgM/Ab Assay.

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Summary of PerkinElmer’s product offerings for COVID-19 research, workflows, detection and vaccine development.

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SuperFlex™ Chemiluminescent Immunoassay System COVID-19PerkinElmer Solutions for Detection and Research

Personal Protective Equipment (PPE)

3-ply level 1 and 3 surgical masks with ear loops. Pack of 50 pieces.

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Medical grade filter. Reusable and eco friendly. TGA approved (332824), CE & FDA approved.

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Level 1 & 3 Surgical Masks KN95 Masks

Class II Biosafety Cabinets

Certified Class II biological safety cabinets are required to process suspected or confirmed SARS-CoV-2 specimens . Scientifix partners with Gelaire Laminar Air Flow to provide reliable biosafety cabinets for the protection of personnel, environment and products in work with microorganism of Risk Group 2 and 3.

Compliance, protection, reliability, and more!

BH-EN hinged-window cabinets embody the latest European control and HEPA filtration technology, together with class-leading ergonomics and functionality.

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UltraSafe electric sash-window cabinets have the same general specification and performance as BH-EN cabinets, but with glass side panels, an electrically-operated sliding window and exhaust discharge on the top.

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BH-EN Class II Biological Safety Cabinets UltraSafe Class II Biological Safety Cabinets

Contact Information:

SCIENTIFIX PTY LTD Freecall: 1800 007 900 Fax: +61 3 9543 7827 info@scientifix.com.au

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VIC / SA / TAS / WA QLD NSW / ACTAmanda Mitchell Idris Mohammed Maureen Chan0487 880 411 0401 649 242 0423 885 649amanda@scientifix.com.au idris@scientifix.com.au maureen@scientifix.com.au

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