secrets of spe

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The Secrets of Solid Phase Extraction (SPE)

for Sample Preparation

Agilent AccuBONDII, AccuBONDII ENV and EVIDEXIISPE



CI0126C 2

What Is Solid-Phase Extraction (SPE)?

• Sample preparation technique with principles similar to those of

HPLC for selective adsorption of analytes or interferences from

complex matrices

• Used for sample cleanup and analyte concentration preceding

LC, GC, ion chromatography and other techniques

• Cost-effective alternative to/replacement for liquid-liquidextraction (productivity, solvent, waste)



CI0126C 3

Advantages of SPE vs. Liquid-Liquid Extraction

• Improved throughput (parallel vs. serial processing)

• Decreased organic solvent usage and waste generation

• Higher and more reproducible recoveries

• Cleaner extracts (contamination, solvent impurities)

• No emulsions

• Tunable selectivity ( SPE phase choices, solvent mixtures)

• Readily automated



CI0126C 4

Typical Applications of SPE

• Sample Cleanup

– Combinatorial reaction cleanup before LC-MS or LC

– Pharmacokinetic studies, dissolution testing

– Isolate analytes from complex matrices – urine, plasma – Remove “column killers” or major interferences

– Eliminate late-eluters to allow isocratic analysis

• Trace Enrichment

– Environmental analysis – Pharmaceutical and Agrochemical applications

• Desalting

• Solvent Exchange

• Sample Preservation and Storage



CI0126C 5

Application and Importance of SPE is Growing

…Rapidly• Applied in cartridge format for 20+ years

—Critically important for sample cleanup and trace enrichment

(esp. environmental and pharmaceutical labs)• SPE usage is accelerating

—Combinatorial synthesis and high-throughput LC-MS analysis

—Overall LC-MS usage increases and greater instrument uptime

—Smaller samples requiring smaller bed masses (tubes and plates)

—Minimization of organic solvents and waste

—Ease of automation (cost, reproducibility, throughput)

• Liquid handling and SPE workstations• 96-well plate autosamplers



CI0126C 6

Typical SPE Formats

Schematic Diagram of a 96-Well SPEExtraction Plate System*

* 3M Corp.

Manifo ld System

Single

Extraction

Disk

SPE Cartridge

Schematic Diagramof a

96-Well Plate Extraction System

(LC-GC, S9, May, 1998)

R. E. Majors, LC-GC, 15(4), 318 (1997)



CI0126C 7

SPE Modes—”Digital Chromatography”

Matrix Adsorption

• Analyte(s) unretained (k ~ 0)

• Matrix retained(k >> 1)

• No preconcentration advantage

• Eluates may not be as clean

• Sample loading often gravity fed

• Used less often than analyteadsorption

Analyte Adsorption

• Analyte(s) retained (k >> 1)

• Matrix unretained(k ~ 0)

and/or strongly retained (k >> 1)

• Preconcentration factor

• Cleaner extracts

• Load at 1-3 drops/sec (recovery ∝1/flow)

• Capacity issues may be moreimportant



CI0126C 8

Fundamental Steps for SPE Adsorption Mode

Prewash*

Remove contaminants that

could elute with analyte

Precondition Load Wash Elute

Prepare cartridge to

accept sample

Load sample and

rinse reservoir (s)

Wash with solvent that

won’ t elute analyte

Elute analyte in smallest

volume possible

If elution solventwill be stronger

than precond. solvent

1. MeOH or ACN

2. Weak solvent(water, buffer )

Weakly retainedmatrix compds elute

Analyte and other

matrix compds

retained

Elute analyte

leaving highly

retained compds

1 2

* N o t n e c

e s s a

r y

f o r A g

i l e n t S P

E



CI0126C 9

General SPE Method Development Strategy

SPE Adsorption Mode: Background

• Research the Problem

—Previous SPE and analysis conditions for the analyte and matrix?

• Characterize the Analyte

—Structure, pKa, polarity, functional groups

—Solvent solubility and stability

• Any restrictions on final solvent and concentration(technique or instrument)?

• Characterize the Sample Matrix

—Possible interferences — similar functional groups, pKa, etc.

—pH, ionic strength —Solvent solubility and stability

—Qualitative and quantitative variability



CI0126C 10

General SPE Method Development Strategy

SPE Adsorption Mode: Experimental

• Develop or apply effective HPLC or GC conditions to monitor progress

— Assess recovery and eluate cleanliness

• Select and test sorbents

—Determine which sorbents provide maximum analyte retention

—Determine which eluent solvents yield highest recoveries

• Identify optimum wash solvent

—Assess eluate cleanliness under conditions of maximum analyteretention

—Determine strongest wash solvent that will not elute analyte

• Test blank and fortified matrix

—Assess eluate cleanliness and recovery using optimum wash and eluentsolvents

• Test real samples and fortified samples

—Assess eluate cleanliness and recovery for different unfortified andfortified samples



CI0126C 11

SPE Method Validation

Variables to Consider

• Validate SPE procedure

—Sorbent (sorbent weight, different cartridges, different lots)

—Preconditioning (strong solvent, weak solvent)

—Loading solvent (% organic, pH, ionic strength, volume)

—Wash solvent (% organic, pH, ionic strength, volume)

• Eluent (volume, % organic …) —Flow rates (loading, wash, elution)

—Linearity and range (different analyte concs. and matrix loadings)

—Analyte stability (loading solvent, eluent)

—Matrix stability (loading solvent)



CI0126C 12

Design Characteristics for SPE Family

• Provide a variety of popular chemistries to meet most

application needs

• Provide silica and PS-DVB base materials

• Provide most popular sizes of cartridges and bulk material for

“self-packers”

• Pre-clean packing, tubes and frits – low organic extractables for high sensitivity work

• Economical for routine applications

• Excellent quality assurance program to ensure consistentresults from batch-to-batch and year-to-year

• Specialty phase for drugs of abuse testing



CI0126C 13

Break Time

For Questions and Answers

Press *1 on Your Phone to

Ask a Question



CI0126C 14

SPE Products from Agilent

AccuBONDII

and EVIDEXII

• Improved AccuBONDII SPE Products

—Solid Phase Extraction Cartridges —Bulk SPE Adsorbents and Accessories

—Method Development Kits

—Applications

• EVIDEXII SPE Specialty Cartridges

—Drugs of Abuse

—General Pharmaceutical Extractions

—Applications



CI0126C 15

AccuBONDII SPE Phases

Sulfonic acidStrong Cation Exchange

(SCX)

Quaternary amineStrong Anion Exchange

(SAX)

Sil ica, -CN, Diol, Amino,

Alumina (acidic, basic, andneutral), Florisil

Normal Phase (polar)

C2, C8, C18, Phenyl,ENV PS-DVB*

Reversed Phase

Phases AvailableSPE Mode

* Newest phase



CI0126C 16

AccuBONDII SPE Cartridges

• Silica and new PS-DVB cartridges available

• Wide variety of cartridge sizes

—100, 200, 500 and 1000 mg

—1, 3, and 6 mL tubes —96 well plates in final development

—Tabless cartridges for automation (i.e. Gilson)

• Excellent quality assurance program

—Random testing of cartridges

• surface characteristics

• packing parameters

—Performance certificate in each box• Low extractables

—Pre-washed packings, frits, and tubes

• Bulk packings available – 25 g bottles



CI0126C 17

AccuBONDII SPE – Low Levels of Extractables

Tube and Frit

min0 4 8 12 16

min0 4 8 12 16

Agilent SPE AccuBONDII

Pre-washed Tube/Frit Only

Waters’ Sep-Pak C18

Tube/Frit Only

Programmed Temperature

GC-FID Analysis

Splitless Injection

Internal Standard

Total = 50 ppm

Total = 5 ppm

Internal Standard



CI0126C 18

AccuBONDII SPE Method Development Kits

• SPE Method Development Kits – groups of phases thatseparate using the same mechanism

—Kit A (Reversed Phase)• C2, CN, Phenyl, C8 and C18

—Kit B (Normal Phase)

• Si, Amino, Diol and CN —Kit C (Ion Exchange)

• SCX, SAX and Amino

• Multiple kits provide a wide variety of options for determiningthe best mechanism and phase type for your analyte andmatrix



CI0126C 19

AccuBONDII SPE Application Notes

• Application Notes for AccuBONDII

—Environmental

• Extraction of Chlorinated Pesticides in Water (SE-1)

• Extraction of Polynuclear Aromatic Hydrocarbons in Water (SE-2)• Extraction of PCB’s in Water (SE-3)

• Extraction of Triazines Using SPE Cartridges

—Pharmaceutical

• Anabolic Steroids in Urine and Serum (SC-1)

• Benzodiazepines in Serum (SC-2)

• Caffeine and Metabolites in Serum (SC-3)

• Barbiturates in Serum (SC-4) —Available online:

www.agilent.com/chem



CI0126C 20

AccuBONDII Example Applications

Triazines in Different Matrices


• Structure, pKa, polarity,functional groups

• Solvent solubility andstability

• Any restrictions on finalsolvent and concentrationdue to technique or

instrument?

Triazines

• Three major species – simazine,atrazine and propazine – all

structurally similar

• Mode of Action: Herbicides

• Practically insoluble in water

• Soil – large number of chargedspecies-adjust pH to retain triazinesand do ion-exchange

• Muscle tissue – large amounts of non-

polar lipids – retain these and elutetriazines using C18

• Corn oil – non-polar glycerides andfatty acids –weakly retained on diolwhile triazines are strongly retained


• Solvent solubility and stability

• pH, ionic strength

• Possible interferences—similar

functional groups, pKa, etc.

• Qualitative and quantitative

variability

N N

N

Cl

NH NH

R1R2



CI0126C 21

SPE Methods for Triazines in Complex Matrices

MethanolMethanol Acetonitrile/K2HPO4ELUTE

HexaneWater Acetic acid,acetonitri le, water,

0.1 M K2HPO4

WASH

Diluted with hexaneDiluted with water Diluted with acetic

acidLOAD

Methanol, hexaneMethanol Acetic acidPRE-TREAT

NoneHomogenized in

methanol

Shaken in

acetonitrileEXTRACTION

DiolODSSCXCARTRIDGE

Corn OilMuscle TissueSoil



CI0126C 22

HPLC Analysis of Triazines Extracted

from Different Matrices

Triazines from Corn Oil

with Diol

Triazines from Muscle Tissue

with ODS

Triazines from Soil

with SCX

Column: C18, 4.6 x 150 mm, 5 mm Mobile Phase: 50% methanol:50% 0.01M K2HPO4 Flow Rate: 2 mL/min

Detection: UV 254 nm Sample: Triazines 1. Simazine 2. Atrazine 3. Propazine



CI0126C 23

AccuBONDII Example Applications

Barbiturates in Serum


• Structure, pKa, polarity,functional groups

• Solvent solubility andstability

• Any restrictions on finalsolvent and concentration

due to technique or instrument?

Barbiturates

• Non-polar side groups make retention

on C18 possible – but retention notstrong

• Mid pH will eliminate charge andimprove retention

• Soluble in water

• Final solvent easy to evaporate or compatible with HPLC

• Serum has many componentscompeting for retention

• Serum will vary from person to person


• Solvent solubility and stability

• pH, ionic strength

• Possible interferences—similar functional groups, pKa, etc.

• Qualitative and quantitative

variability

N

NH

O

O

O

R1

R2

R3



CI0126C 24

Analysis of Barbiturates in Serum

AccuBONDII SPE C18 Cartridge

Solid Phase Extraction Method

CARTRIDGE: AccuBONDII ODS (C18) 6 mL/500 mg

(P/N 188-1356 30/box)

Sample Preparation :

Add 1 mL 0.5M K2HPO4 to 4 mL of serum

Precondition:

5 mL Acetone

5 mL Deionized Water

Load:

5 mL Sample (see sample preparationabove)

Wash

2.0 mL 95% water:5% acetonitrile

Elute:

3.0 mL AcetoneEvaporate and Reconstitute:

Evaporate Acetone in a nitrogen stream at<45°C

Add 200 µL of acetonitrile:water (20/80)



CI0126C 25

Break Time

For Questions and Answers

Press *1 on Your Phone to

Ask a Question



CI0126C 26

HPLC Analysis of Barbiturates Extracted

from Human Serum

Column: C18, 4.6 x 150 mm, 5 mmMobile Phase: Time ACN Water

0 20 80

5 20 80

15 40 60

20 40 60

Flow Rate: 1 mL/min

Detection: UV 235 nmSample: Barbiturates

1. Barbital

2. Allobarbital

3. Aprobarbital4. Phenobarbital

5. Butabarbital

6. Alphenal

7. Hexobarbital

8. Amobarbital

9. Mephobarbital10. Secobarbital



A BONDII SPE P d t F t



CI0126C 31

AccuBONDII SPE Product Features

COA, Packaging and Labeling

• Certificate of analysis with each box —Mass consistency —Flow rate consistency

—Trace metal analysis —Extract cleanliness

—QC chromatogram

• Packaged in tr ilayer laminated bag —Excludes air and water —Ensures clean product

• Individually printed tubes and plates

—Easy identification —Versatility for method development

C tifi t f P f f A BONDII 1



CI0126C 32

Certificate of Performance for AccuBONDII – page 1

Description: AccuBONDII C8 100 mg 1 mL cartridge

Catalog No: 188-0310

Lot No.: AMC18-0X

Run No.: XXXXXXX

This AccuBOND product and sorbent have been manufactured, tested and assembled under the control of an ISO 9001 registered quality system.This AccuBOND SPE product has been subjected to the following QC tests:

Base Silica Characteristics Bonded Silica TestsSurface Area: 546 m2/g Carbon Loading: 13.0%Average Pore Size: 60Å Surface Coverage: 2.5 µmoles/m2Surface pH: 7 Extraction Residue: PassMetal Analysis: Pass Exchange Capacity: N/A

Particle Size Data Packed Cartridge Test

Particle Shape: Irregular Cartridge Flow Resistance: PassAverage Particle Size (µm): 56 Frit Purity Test (GC): PassPercentage of Particles <10 µm: 0.00 Material Weight Check: Pass

Percentage of Particles <25 µm: 1.36Percentage of Particles <90 µm: 4.95



CI0126C 33

Certificate of Performance for AccuBONDII — page 2

T e s t C o n d it io n a n d R e s u lt s

C o n d i t i o n s :

C o lu m n : 4 .6 x 2 5 0 m m

M o b ile P h a s e : 7 0 /3 0 M e O H /H 2 O

F lo w R a te : 2 .0 m l /m in

S p e c i f i c a t i o n s :

K ’ b ip h e n y l = 3 .5 to 6 .5

K ’ to lu e n e = 1 .5 to 3 .5

K ’ a c e to p h e n o n e = 0 .6 to 1 .4

T f u r a c i l = 0 .3 to 1 .5

R e su l ts f o r L o t A M C 8 X -X XK ’ to lu e n e = 4 .5 P e a k 1 = U r a c il

K ’ d i m e th y la n i l in e = 2 .1 P e a k 2 = A c e to p h e n o n e

K ’ n i t r o b e n z e n e = 1 .0 P e a k 3 = T o lu e n e

T f d im e th y la n i l in e = 1 .4 P e a k 4 = B ip h e n y l

N o t i c e :

M a t er ia l S a f e ty D a ta S h e e ts ( M S D S ) a r e a v a i la b l e a t:

h t t p : //w w w .c h e m .a g i le n t .c o m / s c ri p t s/ ca g _ m s d s s e a r c h . a s p

T h i s p r o d u c t h a s p a s s e d a l l A g i le n t T e c h n o l o g i e s , i n c . q u a l it y c o n t r o l s p e c i fi c a t io n s .

QA

QC



EVIDEXII SPE Cartridges for Drugs of Abuse



CI0126C 36

S Ca t dges o ugs o buse

Opiates in Urine

Solid Phase Extraction Method

CARTRIDGE: EVIDEXII 400 mg, 6 mL

(P/N 188-2946, Box of 30)

Precondition:

6 mL methanol 6 mL 0.1 M potassium phosphate (pH 6.0)

Do not let the phase go dry

Load:

Add 3 mL 0.1 M potassium phosphate (pH6.0) to the cartridge

Attach an 8 mL reservoir Add the urine sample

Rinse:

Remove reservoir 3 mL water 3 mL 0.1 M sodium acetate (pH 4.5) 3 mL methanol

Elute:

Place a collection tube beneath cartridge 3 mL methylene chloride/isopropyl alcohol/

NH4OH (78/20/2) Collect the eluate

Codeine30 ng

Morphine30 ng

Codeine

Column: DB-5MS EVDX

Carrier: Helium at 40 cm/sec

Oven: 65 degrees for 1 min, 65-325 degrees at 20 degrees/min

Injector: Splitless, 250°C

Detector: MSD, 300°C transfer line

Clean extracts froma very dirty matrix

Morphine

DOA Recoveries are Reproducible



CI0126C 37

Recovery Levels (x±%, n = 8)

Level in ng/mL 1000 1750 2500 Amphetamine 76 ±4 74 ±2 96 ±3

Methamphetamine 85 ±1 71 ±3 98 ±3

Level in ng/mL 50 87.5 125PCP 90 ±3 91 ±2 88 ±3

Level in ng/mL 300 525 750Benzoylecgonine 98 ±8 93 ±4 97 ±3

Level in ng/mL 600 1050 1500Codeine 99 ±1 97 ±3 98 ±1Morphine 96 ±2 96 ±3 93 ±4

Level in ng/mL 30 52.5 75THC-COOH 92 ±4 96 ±8 94 ±3

p

Using EVIDEXII SPE Cartridges



SPE Tips For Improving Recovery and Precision



CI0126C 40

• General

—Keep cartridges in sealed bags until use.

—Store in zipper-locked bags or in desiccator once opened.

—When using empty reservoirs attached to cartridges, use longdisposable pipets to ensure proper flow from reservoir to cartridge.

—Use stopcocks to adjust/control flow through individual cartridges.

• Use mass balance for all fractions to determine fate of analyte (adsorption

to surfaces, loading effluent, washes, eluate, etc.) during methoddevelopment.

—Residual water can be removed effectively by centrifugation (5000

rpm, 5 min.) compared to drying with vacuum or nitrogen.

—Cartridge capacity for analytes and matrix is typically about 1-3% of cartridge bed weight (ion-exchange not included).

—NEVER allow the cartridge to dry out until the elution step.





CI0126C 41


• Prewash

—Remove all strong prewash solvent for GC (e.g., dichloromethane,

hexane, ethyl acetate) before preconditioning and loading.

• Precondition —Make sure pH is correct for ion-suppression (acids) or minimal

silanol interactions (bases).

—Leave ~1-2 mm of preconditioning solvent above sorbent bed to

prevent bed from drying.

—Leave ~1/4 to 1/2 of tube volume above sorbent bed when using

empty reservoir above cartridge.

SPE Tips For Impro ing Reco er and Precision



CI0126C 42


• Load

—Leave ~1/4 to 1/2 tube volume above sorbent bed when using tube

reservoir above cartridge.

—Use drop wise solvent flow when time/throughput is not a major

concern.

• Wash

—Wipe needles of manifold before elute step to minimize contamination of

eluate.

• Elute

—When choosing eluent, consider ease of evaporation if reconstitution is

needed.

—Allow cartridge/plate to soak with eluent for 0.5 - 1 min. (➚recovery).

—Sometimes several smaller eluent aliquots can improve recovery.



Extraction and Separation of PAHs from Water



CI0126C 44

AccuBONDII ODSSolid Phase Extraction Method

CARTRIDGE: AccuBONDII , 500 mg, 6 mL

(P/N 188-1356, 30/box)

Sample Preparation:

Add 2 mL Isopropanol to 20 mL Water Sample

Mix thoroughly

Precondition:

5 mL Methylene Chloride 5 mL Methanol 5 mL Deionized Water

Add ~1 mL Deionized Water to top of bed

Load:

Attach 24-mL sample reservoir to cartridge

Add sample to reservoir. Ensure flow into cartridge with glass pipet. Apply vacuum and keep flow rate <10 mL/min. Slower flow gives

better results.

Wash:

3 mL 50:50 Acetonitrile/Deionized Water

Apply vacuum until 30 sec. after wash has passed through cartridge Centrifuge cartridge at 1000-1500 rpm for 5 min. (removes excess

water)

Elute:

3 mL Methylene Chloride and collect eluent. Evaporate to 50-200 µL. Do not apply heat or smaller PAHs will be

volatilized. Add Methylene Chloride to bring sample to final volume of 200 µL.

Inject 2 µL.

Compound Avg.Recovery

(%)

Std.Dev.

(%)

Naphthalene 80 12

2-Chlor onaphthalene 78 8

Acenaphthylene 81 9

Fluorene 99 9

Phenanthrene 98 9Fluoranthene 91 8

Chrysene 97 12

Benzo(b)-fluoranthene 60 8

Benzo(a)pyrene 55 8

Indene(1,2,3-c,d)pyrene 60 10

Benzo(g,h,i)perylene 59 10

Water Spiked at 50 ppb

Cl

Typical SPE Extraction Protocol for Opiates



CI0126C 45

Sample Preparation:

• Add 0.5 mL hydrochloric acid to 5 mL urine

• Cap• Heat at 120°C for 20 min

• Cool to room temperature

• Add 0.75 mL 10 N NaOH

• Adjust to pH 6.5-7.5 with ~2.5 mL 0.5 M phosphoric acid

Condition:

• 6 mL methanol

• 6 mL 0.1 M K2HPO4 (pH 6.0)• Do not let the phase go dry

Load:

• Add 3 mL 0.1 M K2HPO4 to the cartridge

• Attach an 8 mL reservoir

• Add the urine sample

Rinse:

• Remove reservoir

• 3 mL water

• 3 mL 0.1 M sodium acetate (pH 4.5)

• 3 mL methanol

Elute:

• Place a collection tube beneath cartridge

• 3 mL methylene chloride/isopropyl alcohol/ NH4OH(78/20/2)

• Collect the eluant

Analysis Preparation:

• Concentrate the eluant to dryness

• Do not over dry• 75 µL ethyl acetate

• 25 µL BSTFA/1% TMCS

• Cap

• Heat at 60°C for 10 min

• Inject 1-2 µL

• All flow rates should not exceed 5 mL/min

Reagents:

• 10 N NaOH

40 g sodium hydroxide in 100 mL DI water.

• 0.1 M K2HPO4 (pH 6.0)1.74 g potassium phosphate, dibasic (anhydrous) in 100 mL DI

water. Adjust

to pH 6.0 ± 0.1 with phosphoric acid.

• 0.1 M sodium acetate (pH 4.5)0.82 g in 100 mL DI water. Adjust to

pH 4.5 ± 0.1 with glacial acetic acid.

• Methylene chloride/isopropyl

alcohol/NH4OH (78/20/2)

78 mL methylene chloride, 20 mL

isopropyl alcohol, 2 mL ammonium hydroxide. Make fresh daily.

• BSTFA/1% TMCS

9 mL N,O-bis(trimethylsilyl)trifluoroacetamide, 0.1 mLtrimethylchlorosilane, or can be purchased premixed.

Other reagents

Methanol

DI water

Ethyl acetate

Hydrochloric acid

Phosphoric acid

Certificate of Performance for AccuBONDII



CI0126C 46

Description: AccuBOND C8 100mg 1mL cartridgeCatalog No: 188-0310

Lot No.: AMC18-0XRun No.: XXXXXXX

This AccuBOND product and sorbent have been manufactured, testedAnd assembled under the control of an ISO 9001 registered quality system.

This AccuBOND SPE product has been subjected to the following Q.C. tests:

Base Silica Characteristics Bonded Silica Tests

Surface Area: 546 m2/g Carbon Loading: 13.0 %Average Pore Size: 60 Å Surface Coverage: 2.5 µmoles/mSurface pH: 7 Extraction Residue: PassMetal Analysis: Pass Exchange Capacity: N/A

Particle Size Data

Particle Shape: Irregular Average Particle Size (µm): 56Percentage of Particles <10µm: 0.00Percentage of Particles <25µm: 1.36

Packed Cartridge Test

Cartridge Flow Resistance: PassCartridge Purity Test (GC): PassFrit Purity Test (GC): PassMaterial Weight Check: Pass

Percentage of Particles >90µm: 4.95

Test Condition and Results

Conditions:Column: 4.6 x 250 mm

Mobile Phase: 70/30 MeOH/H2O

Flow Rate: 2.0 ml/min

Specifications:

K’ biphenyl = 3.5 to 6.5

K’ toluene = 1.5 to 3.5

K’ acetophenone = 0.6 to 1.4

Tf uracil = 0.3 to 1.5

Results for Lot AMC8X-XX

K’ toluene = 4.5 Peak 1 = Uracil

K’ dimethylaniline = 2.1 Peak 2 = Acetophenone

K’ nitrobenzene = 1.0 Peak 3 = Toluene

Tf dimethylaniline = 1.4 Peak 4 = Biphenyl

Notice:

Material Safety Data Sheets (MSDS) are available at:

http://www.chem.agilent.com/scripts/cag_msdssearch.asp

This product has passed all Agilent Technologies, inc. quality control specifications.

2

QA

QC

HPLCColumnTechnicalSupport



CI0126C 47

HPLC Column Technical Support

800-227-9770 (phone: US & Canada)*302-993-5304 (phone)*

* Select option 4, then option 2 .

916-608-1964 (fax)

www.agilent.com/chem

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