swachandabhiravras rs006 gdg
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Pharmaceutico analytical study on Swacchandda Bhairava rasa (Dwitiya)” - Dr. Anand. H, Department of rasashastra, Post graduate studies and research center, Shri D. G. Melmalagi Ayurvedic Medical College, GadagTRANSCRIPT
“Pharmaceutico-Analytical study on Swacchandda
Bhairava rasa (Dwitiya)”
BY
DDRR.. AANNAANNDD.. HH..
Dissertation Submitted to the Rajiv Gandhi University Of Health Sciences,
Karnataka, Bangalore.
In partial fulfillment of the requirements for the degree of
AAYYUURRVVEEDDAA VVAACCHHAASSPPAATTII ((DDOOCCTTOORR OOFF MMEEDDIICCIINNEE))
IN RASASHASTRA
Under the guidance of
DR.DILIP KUMAR.B. M.D. (RASASHSATRA) P.G.Dept. of Rasashastra
and Co-guidance of
DR. GIRISH. N. DANAPPAGOUDAR, M.D. (RASASHASTRA),
P.G.Dept. of Rasashastra
POST GRADUATE DEPARTMENT OF RASASHASTRA D.G MELMALAGI AYURVEDIC MEDICAL COLLEGE AND RESEARCH CENTER
GADAG – 582103 2006
Rajiv Gandhi University Of Health Sciences, Karnataka, Bangalore.
DECLARATION BY THE CANDIDATE
I here by declare that this dissertation / thesis entitled “Pharmaceutico
analytical study on Swacchandda Bhairava rasa (Dwitiya)” is a bonafide
and genuine research work carried out by me under the guidance of Dr.Dilipkumar P,
M.D.(Ayu), (Rasashastra), Assistant-Professor, Post graduate department of Rasashastra
and under the Co-guidance of Dr. Girish. N. Danappagoudar,M.D. (Rasashastra).
Lecturer, Post graduate department of Rasashastra.
Date: Place: Gadag. Dr.Anand. H
SHRI D. G. MELMALAGI AYURVEDIC MEDICAL COLLEGE, POST GRADUATE DEPARTMENT OF RASASHASTRA.
CERTIFICATE BY THE GUIDE
This is to certify that the dissertation entitled “Pharmaceutico -
Analytical Study on Swaccanddha Bhirava Rasa(Dwitiya) .” is a bonafide
research work done by Dr. Anand. H. in partial fulfillment of the requirement
for the degree of Ayurveda Vachaspathi. M.D (Rasashastra).
Date: Place: Gadag. Dr. Dilipkumar. B. M.D. (Ayu)
Astt Professor Department of Rasashastra,
D.G.M.A.M.C, Gadag.
SHRI D. G. MELMALAGI AYURVEDIC MEDICAL COLLEGE,
POST GRADUATE DEPARTMENT OF RASASHASTRA.
CERTIFICATE BY THE Co - GUIDE
This is to certify that the dissertation entitled “Pharmaceutico
analytical study on Swacchandda Bhairava rasa (Dwitiya)” is a bonafide
research work done by Dr. ANAND.H. in partial fulfillment of the requirement
for the degree of Ayurveda Vachaspathi. M.D (Rasashastra).
Date:
Place: Gadag. Dr. Girish. N. Danappagoudar, M.D. (Rasashastra).
Lecturer,
Postgraduate department of Rasashastra.
ENDORSEMENT BY THE H.O.D AND PRINCIPAL OF
THE INSTITUTION
This is to certify that the dissertation entitled “Pharmaceutico analytical
study on Swacchandda Bhairava rasa (Dwitiya)” is a bonafide research
work done by Dr. ANAND.H. under the guidance of DR. Dilipkumar. B M.D.
(Rasashastra), Asst. Professor, Postgraduate department of Rasashastra and co-
guidance of Dr. Girish. N. Danappagoudar, M.D. (Rasashastra), lecturer, Postgraduate
department of Rasashastra.
DR. M.C.Patil, M.D. (Rasashastra) Dr. G. B. Patil.
Professor & H.O.D, Principal.
Post graduate department of Rasashastra. D.G.M.A.M.C, GADAG.
D.G.M.A.M.C, GADAG. Date: Place: Gadag
COPYRIGHT
Declaration by the candidate
I hereby declare that the Rajiv Gandhi University of Health Sciences,
Karnataka shall have the rights to preserve, use and disseminate this
dissertation / thesis in print or electronic format for academic / research
purpose.
Date: Signature of Scholar
Place: Gadag
Dr.Anand.H
© Rajiv Gandhi University of Health Sciences, Karnataka.
ABSTRACT
Background.
Rasaoushadhis with different formulations are classified according to mode of
preparation under khalvi rasayana, kupipakva rasayana, pottali rasayana and parpati rasayana.
Rasaoushadhis with various elements along with kajjali is proved more effective than herbal
formulations in lesser dosage
Swacchandda Bhairava rasa (Dwitiya) is a khalvi rasayana, with a unique herbomineral
combination of drugs to treat Navajwara. Before its clinical application it is the need of hour to
critically undergo pharmaceutical and analytical evaluation as a part of safety measures. Hence
study is under taken.
Objectives :
• Preparation of Swacchandda Bhairava rasa (Dwitiya).
• Physico-chemical analysis of Swacchandda Bhairava rasa (Dwitiya).
Methods:
Pharmaceutical study.
1.Hingula shodhana- Rasatarangini, 9th chapter, Sloka – 16 – 17.
2.Hingulotha parada- Rasatarangini, 9th chapter, Sloka – 13 – 17.
3.Parada shodhana- Rasatarangini, 5th chapter, Sloka – 40 – 42.
4.Gandhaka shodhana- Rasatarangini, 8th chapter, Sloka – 7 – 11.
5.Kajjali preparation- Rasatarangini, 6th chapter, Sloka – 110 – 111.
6.Vatsanabha shodhana- Rasatarangini, 24th chapter, Sloka – 19 – 25.
7.preparation of Swacchandda Bhairava rasa (Dwitiya)- Bhaisajyarathnavali, 5th chapter, Sloka –
492- 493.
ABSTRACT - IV
Analytical study:
Swacchandda Bhairava rasa (Dwitiya) is subjected to phyisico-chemical analysis like
organoleptic characters,pH value, total ash value, fineness, estimation of mercury and sulphur.
Interpretation and conclusion:
1.Kajjali is a black sulphide of parada, which is a sagandha, niragni pota bandha of parada,
khalvi rasayana.
2.Trituration time and saturation of parada with gandhaka is directly propotional to the
pharmacological effect of kajjali and its further complex compounds
3. Swacchandda Bhairava rasa (Dwitiya) is one among Sagandha Niragni Murchita paradayoga.
When purified Mercury and sulphur are intimately mixed in a definite proportion to get a black
powder called as Kajjali.
4.Siddhi laxanas of ayurveda and modern physico-chemical analysis are confirmative test to
evaluate the perfectness of the pharmaceutics.
Keywords:
Hingula, hingulotha parada, parada, gandhaka,kajjali,vatsanabha, pippali,
jatikosha,Swacchandda Bhairava rasa (Dwitiya).
ABSTRACT - V
ACKNOWLEDGMENT
First and foremost, I Salute almighty God, by his blessings and Grace, which
gives us success in life.
My deep sense of gratification is due for my parents, my uncle, and family
who are the architects of my career.
I am extremely happy to express my deepest sense of gratitude to my beloved
and respected Guide, Dr. Dilip kumar B. M.D. (Rasashastra)) whose sympathetic, scholarly
suggestions and Guidance at every step have inspired me not only to accomplish this
work but in all aspects.
I express my deep gratitude to my respected Sir H.O.D. and Prof. Dr. M.C.
Patil M.D. (Rasashastra) for his critical suggestions and expert guidance for the completion
of thesis.
I am extremely greatful to my Co-guide Dr. Girish. N. Danappagoudar,M.D.
(Rasashastra). Lecturer, Postgraduate department of Rasashastra, under whose
guidance and valuable suggestions, I have been able to complete this research work.
I offer my sincere thanks to Dr. R.K. Gaccchinmath, professor and HOD, UG
Dept of Rasashastra, DGMAMC, Gadag, for his constant support
I express my deep sense of gratification to my beloved and Respected sirs, , Dr
Jagadeesh Mitti, MD (Ayu) Lecturer, PG Dept of Rasashastra, DGMAMC, Gadag,.
Whose guidance, inspiration, supervision and valuable suggestions, I have been able
to complete this Research work.
I express my deep gratitude to beloved Principal Dr. G.B. Patil, Principal
DGMAMC, Gadag, for his encouragement and providing all necessary facilities for
this research work.
ACKNOWLEDGMENT - I
I express my deep gratitude to Dr.Guru.Rau, Director SSCU,IISc.,
Dr.Shivukumar, Dept of XRD,IISc, Dr.Kannan,Dept of Emax,IISc.Bangalore.
I take this opportunity to thank Shri Chandur, Lecturer K.L.E’s College of
Pharmacy Gadag, who extended valuable support by conducting analytical
procedures.
I express my deep gratitude to Shri B.C. Hatapakki M.Pharm, Principal
K.L.E.’s College of Pharmacy Gadag, for his encouragement and providing all
necessary facilities for this research work..
I express my sincere thanks to Dr. Varadacharylu M.D. (Ayu), Dr. Mulagund M.D. ,
Dr. G. Purushothamacharylu M.D. (Ayu) for their constant support.
I am grateful to Dr. K.S.R. Prasad M.D. (Ayu), Dr. Shivramudu M.D. (Ayu), Dr. S.H.
Doddamani M.D. (Ayu), Dr. R.V. Shettar M.D. (Ayu), Dr. Kuber sankh M.D. (Ayu), Dr. Santosh
Belvadi M.D. (Ayu), Dr. Sashikanth Nidagundi M.D. (Ayu), and other P.G. Staff for their
constant encouragement.
I extend my gratitude to Shri V.M. Mundinmani and Sureban for providing the
required books during the study.
With great pleasure I offer my recognition to my friends Dr. Suvarna P.
Nidagundi, Dr. Anitha H, Dr and Dr. Shambulinga Teggi for their friendly affection
and amiable attitude during my study period without which I would never be
complete.
I offer my sincere thanks to my friends Dr. M.V. Sobagin, Dr. B.Y. Ghanti,
Dr. Pradeep, Dr. Shankuntala and Dr. M.S. Hiremath for their kind co-operation and
help.
ACKNOWLEDGMENT - II
I offer my sincere thanks to my senior friends Dr. Santoji, Dr. Koteshwara, Dr,
V.S. Hiremath, Dr. Jaggal, Dr Sharanabasappa. S. Dr. R.B. Pattanshetty, for their
immense help and affection.
I am also thankful to my junior friends Dr. Rudrakshi, Dr. Suma Jamakhandi,
Dr. Jaya, Dr. Kattimani, Dr. Shivakumar, Dr. Ravindra, Dr. Anupama Bijjal, Dr.
Sarvamangala, Dr. Kavitha for their support and affection.
I am grateful to Shri Chaitrakumar (Sadguru computers) for his kind co-
operation and immense help to complete the dissertation work.
My sincere thanks to my batch mates Dr. Suresh, Dr. Manju, Dr. Ashwin, Dr.
Gavi, Dr. Survey, Dr. Krishna, Dr. Chanveer, Dr. Vijay, Dr. Jagadeesh, Dr. Umesh,
Dr. Reddy, and all my friends for their kind support.
Lastly but not least my heartly thanks to Dr.Madhav.Diggavi for his kind
suggestions.
Dr. ANAND.H.
ACKNOWLEDGMENT - III
CONTENTS
Chapter Page No.
Introduction 1 - 2
Objectives 3
Review of literature 4 - 52
Methodology 53 - 83
Discussion 84 - 91
Conclusion 92 - 93
Summary 94
Bibliographic References 95 - 110
CONTENTS - VI
LIST OF ABBREVIATIONS
⇒ A. P. – Ayurveda Prakasha.
⇒ B.P. - Bhava prakasha.
⇒ B. P.N. – Bhava Prakasha Nighantu.
⇒ C.S. - Charaka Samhita.
⇒ D.N. - Dhanvantari Nighantu.
⇒ M.M. - Materia medica.
⇒ M.P.N. - Madanapal Nighantu.
⇒ R. N. – Raja Nighantu.
⇒ R. M. – Rasamrita.
⇒ R. S.S. – Rasendra sara sangraha.
⇒ R.R.S. - Rasaratna Samuchchaya.
⇒ R. T. – Rasatarangini.
⇒ R. J.N. – Rasa Jala Nidhi.
⇒ S.S. - Sushruta Samhita.
⇒ Y. K. – Yoga Ratanakara.
ABBREVIATIONS - VII
LIST OF TABLES Sl. No. Contents Page No.
1 Table showing the best variety and lakshanas of hingula 6
2 Table showing the synonyms of Parada 13
3 Table showing the varieties of Parada 14
4 Table showing the yougika doshas and their effects 15
5 Table showing the kanchuka doshas and their effects 16
6 Table showing the types of Gandhaka 22
7 Table showing the types of Gandhaka, qualities and uses 22
8 Table showing the synonyms of Vatsanabha 34
9 Table showing the Vatsanabha Bheda, Acc. to Yogaratnakara. 36
10 Table showing the Vatsanabha shodhana, Acc. to different authors 38
11 Table showing the synonyms of Pippali 44
12 Table showing the synonyms of Jatikosha 49
13 Table showing the Actions of Jatikosha 49
14 Table showing the indications of Jatikosha 50
15 Table showing the Results of Hingula shodhana 54
16 Table showing the quantity of Hingula, before & after shodhana 55
17 Table showing the observations during Hingulotta parada 57
18 Table showing the observations during shodhana of parada 60
19 Table showing the quantity of Gandhaka before & after shodhana 61
20 Table showing the different phases of Kajjali during preparation 63
21 Table showing the physical properties of Kajjali 63
22 Table showing the quantity of Vatsanabha, before & after shodhana 66
23 Table showing the quantity of Vatsanabha, before & after
churnikarana
67
24 Table showing the quantity of Pippali, before & after churnikarana 68
25 Table showing the quantity of Jatikosha, before & after churnikarana 69
26 Table showing the ingredients of Swacchandda bhairava rasa
(Dwitiya), with their proportions
70
LIST OF PHOTOGRAPHS
Sl. No. Contents 1. Ingredients of Swacchandda bhairava rasa (Dwitiya) 2. Ingredients of Swacchandda bhairava rasa (Dwitiya)
LIST OF TABLES - VIII
Introduction
INTRODUCTION
The concept of rasaushadhies is come in existence along with the evaluation of
different pharmaceutical processes in the field of ayurveda. These processes makes the
material into such a suitable form which acts on different non manageable diseases, in
minute dosages, quick effectiveness, having long shelf life compaired to other drugs.
Rasashastra a branch of Ayurveda deals with metals, minerals, etc. Rasaushadhies are
mainly grouped into four varieties –
1. Kharaliya rasayana
2. Pottali rasayana
3. Parpati rasayana
4. Kupipakwa rasayana
Method of preparation of Rasaushadhies are different from each other. Kharaliya
rasayana occupies major proportion in the Rasa rasayanas. In that four rasayanas
Kupipakwa and pottali rasayanas are limited. But kharaliya rasayanas are numerous.
Kharaliya rasayanas are prepared by mercurial compounds like Kajjali, Hingula, etc.
Mardana is done in Khalva yantra with dhatu, bhasmas, vishas, upavishas, sadharana
rasa. Bhavana is given with dugdha, jala, swarasa. It can be used as churna or vati. Here
no agni samskara is given and yogas which are prepared in khalva yantra are called as
kharaliya rasayana.
Swacchandda Bhairava rasa (Dwitiya), which comes under kharaliya rasayana a unique
herbomineral formulation to treat Navajwara.
The potency of yoga is dependent on the proper classical pharmaceutical procedure
adopted. Along with it the drugs also plays an important role. The pharmaceutical
procedures like shodhana, mardhana make the drug into such a state that it is easily
absorbed and assimilate in the body and produce its desired effects.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 1
Introduction
Analytical procedures are safety measures to confirm the drug quality.
Parada and gandhaka is considered as a supreme drugs in rasashastra, their combination
in kajjali form are base for almost all formulations.
Further kajjali with any herbal powder when stratified with khalvi rasayana method will
become a new complex chemically. The shelf life of the herbal active chemical molecules
will be maintained for longer period due to the inert kajjali effect.
Hence Swacchandda Bhairava rasa (Dwitiya) is taken for the present study to establish its
pharmaceutical and analytical approach.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 2
Objectives
OBJECTIVES
1.Preparation of Swacchandda Bhairava rasa (Dwitiya).
2.Physico-chemical analysis of Swacchandda Bhairava rasa (Dwitiya).
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 3
Review of Literature
Review of Literature
The main aim of Rasa Shastra is not Lohavada, but to attain Jeevan Mukti by
means of Deha vada, for which contribution of Rasoushadhis to make the body
disease free and healthy is versatile.
Mercury is considered as semen of Lord Shiva which can perform anything
effectively with Rajas of Parvathi (Shakti) i.e. sulphur. Haragowri sristi samyoga is
needed to achieve jeevanmukti. This philosophical basis leads Rasasiddhas to perform
various experiments. As a result, various substances emerged with the combination
of Parada and Gandhaka as mainstream of medicine.
Swaccanddha bhirava rasa (dwitiya)1 is a rasagandha kajjali yoga,which
contains Parada Gandhaka Vatsanabha pippali and Jatikosha. The present review has
collectioin from classical and contemporary references. This chapter is divided under
three headings i.e., Drug Review, Pharmaceutical Review and Analytical Review.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 4
Review of Literature
DRUG REVIEW
HINGULA
Hingula is the main source of Parada. Parada extracted from Hingula is said to be
equivalent to Ashtasamskarita Parada.
Hingula is one of the Sadharana Rasa2.
Varga authors
Maharasa Rasahrudaya Tantra and Rasakamadhenu
Rasagarbha Anandkanda
Rasa gandhaka sambhava Rasarnava
History:
The reference of Hingula is found in Kautilya Arthashastra in testing of Gold and
spoilage of Gold.
Occurrence: Italy, France, Germany, Spain, China, Japan and Iran.
Synonyms3:
Darada, Shukatunda, Hingala, Hingula, Ingala, Mleccha, Rakta, Suranga, Chitranga,
Churna Parada, Rasodbhava, Rasasthana, Rakta Kaya, Kapishirshaka.
Vernacular Names4:
Sanskrit: Hingula ; Hindi : Hingul, Singarph ;
Assami: Janjaphar ; Gujrati : Hingula ; Marati : Hingula ;
Kannada: Hingalika ; Telugu : Ingalikam ;
English: Cinnabar
Varieties5, 6,7:
On the basis of occurrence
Khanija (Mineral) Krutrima (Artificial)
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 5
Review of Literature
On the basis of colour and properties:
Charmara : Krishna, Rakta Varna
Shukatunda : Pita Varna
Hamsa Pada : Japa Kusuma Varna
In the above varities, Hamsapada is considered as uttama and Charmara as adhama.
Grahya Lakshana8, 9:
Table No. 1, Showing the best variety and Lakshanas of Hingula.
Japakusuma Varnabha Resembles colour of petals of red hibiscus flower.
Peshane Sumanoharaha When grinded its colour becomes beautiful
Mahojwala Reflects in sunlight
Bharapurna Heavy in weight
Shweta Rekha Having white or silvery streaks
Pravalabha Resembles like that of Pravala.
Hingula Shodhana10,11:
Hingula gets purified by subjecting it to -
Seven Bhavanas with Ardraka or Lakucha Swarasa
Seven Bhavanas with Nimbu Swarasa.
Bhavana with meshi Kshira
Gunas of Hingula 12:
Rasa : Tikta, Katu, Kashaya
Guna : Ushna
Veerya : Ushna
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 6
Review of Literature
Shuddha Hingula is useful in the treatment of Prameha, Kushta, Jwara, Mandagni,
Hrdroga, Aruchi, Amlapitta, Hrullasa, Pliharoga, Amavata and also useful in treating
Gara Visha.
Hingula is used in the Marana of Gold, Iron etc, metals. Mercury extracted from
Hingula is considered equal in properties to the mercury in which Gandhaka Jarana
has been made.
Matra13, - ½ - 1 Ratti
Anupana - Maricha, Guda, Pippali, Guduchi
Swarasa, Madhu.
Important Yogas of Hingula
Some of the important yogas are Ananda Bhairava Rasa, Kanaka Sundara Rasa, Jwara
Murari Rasa, Vasantha malati Rasa, Ratna Garbha Pottali Rasa, Tribhuvana Kirti
Rasa, Kasturi Bhairava Rasa, Hinguleshwara Rasa.
HINGULA MODERN VIEW14
(CINNABAR)
Cinnabar is the chief ore of Mercury contains 80-85% of Mercury. This is a red
coloured ore, divides itself into pointed needle like pieces. It is very soft and when
ground it becomes deep red coloured.
It occurs both in crystalline and massive forms. When used as pigment it is called
vermilion.
Occurrence: Occurs naturally as a mineral and also prepared artificially. It occurs
naturally in Spain, Italy, France, Germany, China, Japan, Russia and Iran. Artificial
cinnabar is prepared in India in Surat and Kolkata but there is no natural source
available in India.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 7
Review of Literature
General Description of Cinnabar:
Chemical formula : Hgs
Colour : Cochineal red
(Red Sulphide of Mercury)
Streak : Scarlet
Hardness : 2.5
Specific gravity : 8.1
Cleavage : Prismatic perfect
Fracture : Subconchoidal to uneven
Cinnabar classification :
Dana Class - Contains sulfides including sclenides and telluride’s
Strunz class - Contains sulfides and sulpho salts
Preparation of Artificial cinnabar15:
Parada and Gandhaka are taken in 6:1 ratio, triturated well, kept in Iron vessel and
heated on tivragni. Then red coloured compound is formed on the upper part is
collected and is called cinnabar.
HINGULOTTHA PARADA16
Hingula is the main ore of Parada. The Parada, which is extracted from Hingula, is
pure and devoid of Sapta-Kanchuka doshas and has the qualities of Samaguna
Gandhaka Jirna Parada. It is equal to Asta Samskarita Parada.
Methods employed:
Various methods have been employed for the extraction of parada from hingula:
Urdhwapatana vidhi17.
Adhahpatana vidhi18
Tiryak patanavidhi
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 8
Review of Literature
Nadayantra etc.
Yantras used for the extraction of parada are:
Patana yantra
Vidhyadhara yantra
Damaru yantra.
Adhapatana yantra
Tiryakpataha yantra
Nada yantra etc
Before extracting parada from Hingula, Hingula must be triturated with juices of
either Nimbu, paribhadra, Nimba patra at least for 3 hrs.juice of citrus fruit which
enables to reduce the Hingula to its fine state of division, by this maximum amount of
parada can be extracted.after trituration chakricas are made and dried kept in any of
the above said yantra and sealed well. Then heated by keeping cold pads over
the top of urdhvapatana yantra, by which parada coalesces over the
inner surface of the upper vessel of damaru yantra.
Cooling the other pot where parada is condensed. Mercury is extracted and squeezed
through the cloth.
Adhahpatana Vidhi:
Hingula is triturated with ardraka or Nimbu swarasa, paste is applied to the Inner
surface of the upper pot.
Lower pot filled with water is placed in the earth. And mouth of upper pot is placed
over the mouth of the lower pot.
Sandhi bandhana is done and dried
Vanopalas are placed over upper pot and fire is given.
After swanga sheeta, Parada is collected from lower pot and washed.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 9
Review of Literature
Tiryak Patana vidhi: By distillation apparatus
Urdhwa Patana Vidhi:
Nimbu Rasabhavita Hingula is made into chakrikas and kept inside the lower pot.
Then closed with another large sized pot over the lower pots mouth and
Sandhibanadhana is done. Lower pot is heated over stove and the upper pot is kept
cool by keeping wet cloth over it.
After complete extraction and swanga Sheeta, Parada along with soot is collected
from the upper pot and filtered to separate the parada.
As the boiling point of Parada is 3570C, for the extraction of Parada from Hingula
6500C – 7000C temperature is required, since dissociation of Hingula occurs at around
6200C
Brief description of modern methods:19
From ancient description it is very clear that the source of extraction of
mercury was only hingula (cinnabar). In Spain, Italy etc., parada is
extracted from hingula by various methods.
First i t was heated with oxygen.
Second method was heating of hingula with Loha (Fe) or Sudha (Ca). By
these two methods most part of the parada separates from sulphur and remained
parada is taken out by distillation. For this purpose various types of furnaces are
employed. There is a vast change in the methodology and equipments employed for
this purpose now a day.
The equation of heating hingula in air is as follows Hgs+O2 -->
Hg + So2 and heating with calcium and ferrum is as follows: -
4 Hgs + 4Cao ----- 4Hg + 3 Cas + Ca So4 .
Hgs + Fe + O2 ----- Hg + Fe + So2.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 10
Review of Literature
EXTRACTION OF MERCURY FROM CINNABAR
Consists of 2 steps
Ore concentration Roasting and distillation
Isolation of Mercury occurs as:
2HgS + 3O2 → 2Hgo + 2So2
2 Hgo → 2Hg + o2
After these methods the remnant-unseparated part of mercury is
obtained by distillation. This process of distillation is called vacuum
distillation. In purification of mercury reduced pressure and vacuum
distillation is major invention. Now a days, readymade equipments are
available for this purpose. This is the brief description of extraction of
parada from hingula.
In present study the above method was employed for the extraction of Parada from
Hingula
Expected out put:
Good quality cinnabar contains about 85% of mercury. An ideally followed
method should yield at least 75-80% of mercury.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 11
Review of Literature
PARADA
Parada has been recognized vastly in the field of Rasashastra. We come across
much quotations providing the importance of Parada in all texts of Rasashastra.
Parada with its various properties, it has important role to play in Rasashastra
pharmacology.
History:
Rasa has been described to be a Devine origin and claimed to be related to
Lord Shiva or Hara. Initially it was used for Alchemical purposes (loha vada) to
convert lower metals like Lead, Tin, Copper, etc. into noble metals like Gold, silver
etc. Later on its therapeutic use in curing the diseases has been recognized.
In Koutilya Arthashastra (325 cent B.C), it is mentioned that swarna can be
prepared by parada20.
In Charaka Samhita there is usage of Parada with Makshika and Gandhaka in
Kushta Roga and it is used externally21.
In Sushruta Samhita its external use has been mentioned22.
Vernacular names23:
Sanskrit - Parada, Assami - Jivaka, English - Mercury, Quick silver, Kannada
– Paraja, Hindi – Para, Marati – Paara, Bangla – Paara, Latin – Hydrargirum (Hg).
Etymological significance of Synonyms24, 25:
Rasa – As it digests all drugs, Nourishes all Dhatu’s of the body. Being
ingested by human for Rasayanartha
Rasendra - King of all medicines or Rasa’s
Suta - Since used for Deha and Loha Siddhi
Parada - Gives an end to sufferings.
Mishraka - Properties of all metals are found in it.
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Table No.2, showing synonyms of Parada based on the following 26
Swarupaatma
Dharmika Devatmaka
Gatyatmaka
Dehavada tmaka
Dhatuvadatmaka
Vishista guna
Darshanika Adyatmika
Galadroupa
nibham
Mahavanhi
Mahateja
Suvarna
Trinetra
Trilochana
Deva
Dehaja
Prabhu
Rudraja
Rajasmala
Shanta
Shiva
Shiva
veerya
Skandha
Harateja
Harabeeja
Harareta
Shivabeeja
Divyarasa
Kechara
Chapala
Chala
Dhurtaka
Amrita
Dehada
Paramamrit
a
Parata
Parada
Mrityunasha
na
Rasayana
Rasayana
sreshta.
Maharasa
Rasa
Rasendra
Rasesha
Rasottama
Rasadhatu
Rasaraja
Rasaleha
Siddadhatu
Soota
Sootaka
Sootarath
Mishraka
Ananta
Kalikant
aka
Sukshm
a
Soubhag
ya.
Jeeva
Jaiva
Divya
Achintya
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Varieties27:
The Varieties of Parada described in various texts based on following factors:
Depending on the colour.
Depending on the impurities and uses of Parada.
Table No. 3, showing varieties of Parada.
Variety Colour Impurities Uses
Rasa
Rakta
Which is free from all types
of impurities
Rasayana
Rasendra Peeta Free from impurities Rasayana
Suta Ishat Peeta With impurities Deharogahara
Parada Shweta With impurities Sarvarogahara
Mishraka Mayura
Chandrika
varna
With impurities Sarvasiddhidayaka.
Doshas of Mercury28:
According to different rasa classics Doshas of Parada are explained as follows:
Naisargika doshas (Natural impurities).
Yougika doshas (Physical impurities)
Oupadika doshas (Chemical impurities in the form of coating).
1. Naisargika Doshas29:
Mercury, which is occurring in native compound form generally, attributes
some impurities due to its natural power of amalgamation. As these impurities occur
due to nature, these doshas are known as “Naisargika doshas”.
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Naisargika dosha Effects.
Visha - Mrutyukara
Vahni - Santapakara
Mala - Murchakara
2. Yougika doshas30
The impurities mixed by the traders from the commercial point of view to
increase the weight of Parada by adding some Ariloha’s.
Ex: Naga, Vanga etc.,
Table No. 4, showing Yougikadoshas and their effects according to different
authors.
Sl. No. Textual
Reference Doshas Effects
1. RRS Naga, Vanga Jadatva Adhmana
2. AK Naga, Vanga,
Visha
Jadhya Pootigandhatva
Mrutyu.
3. A P Naga, Vanga Jadhya, Adhmana
Kushta.
Kanchuka Doshas31
Literally Kanchuka means thin layer. Kanchuka doshas are the impurities of
mercury, which are seen as thin layer covering it. This is due to tarnishing of mercury.
There is some difference of opinion amongst ancient scholars regarding their
name and source but all of them considered as seven in number.
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Table No. 5 Showing Kanchuka Doshas and their effects according to different
Rasa classics.
Sl.No. Text Doshas Effects
1. Parpati Mrunmaya (Prithvi) Kushta,
2. Patini Pashanaja (Girija) Jadhya, Admana
3. Bhedi Jalaja (Varija) Vali, Palita, khalitya, Vaksangatha,
Mala Bhedana.
4. Dravi Nagaja (Shyama) Mahakusta, Sweta Kusta, Udara,
Kamala, Pandu, Prameha.
5. Malakari Nagaja (Kapalika) Dadru, Gaja Karna, Doshavardhaka.
6. Dhwankshi Vangaja (Kapali) Swara Parushyakara.
7. Andhakari Vangaj (Kalika) Marmacheda, Vastishoola, Andhatva.
Grahya Lakshanas of Parada32:
Parada is liquid in form, shines as bright as mid – day sun, white glaze
exteriorly and bluish tinge interiorly mercury with these qualities is known as Grahya
variety.
Agrahya Lakshanas of Parada33:
Mercury looking smoky, grayish and slightly yellowish or having various
shades of colours is agrahya variety, incorporated with various metallic and elemental
impurities bonded physico – chemically.
Parada Shodhana
Samanya shodhana of parada:
Shodhana is intended to get rid of impurities of Parada. As the Parada is obtained
from the earths crust naturally it adsorbs some unwanted soil particles & chemical
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over it. So it is essential to carry out some purifactory procedures before making use
of parada.
Different Methods Adopted for Samanya Shodhana of parada:
Some texts advised urdvapathana Samskara i.e distillation followed by sublimation of
parad.
Some authors mentioned just filtration through two to four folded fine, dust free, silk
cloth for 7-21 times.
Parada should be triturated with Nagavalli swarasa, Ardraka swarasa, ksharatraya for
3 days and washed with water. This parada will be shining like mukta and devoid of
sapta dosha.
Parada should be triturated with lasuna and saindhava lavana on a tapta khalva yantra
for 7 days.
Method employed in the present study for samanya shodhana34 -
The parada, which is extracted by urdhwa patana vidhi by hingula, is devoid
of Sapta kanchuka dosha is subjected to shodhana. Parada’s 1/16th part of Haridra
churha and Nimbu swarasa-Q.S is taken in a porcelain dish and triturated for 2 days.
After drying, it is filtered through four-folded cloth and parada is procured.
Drugs mentioned for Samanya Shodhana of Parada :
Parada shodhana has to be carried out for 3 to 7 days, in any of the following
drugs to get rid of parada doshas.
Kumari, Chitraka, Raktasarshapa, Haridra, Ishtika choorna, Triphala, Nagavalli
swarasa, Gruha Dhooma, Kanji, Ardraka swarasa etc.,
Vishesha Shodhana
This procedure was intended for strengthening and potentiation of Parada and
is achieved by Astadasha Samskaras.
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Pharmacological and therapeutic properties of Parada35:
Rasa : Shadrasa
Guna : Snigdha, Sara and Guru
Veerya : Ushna
Vipaka : Madhura
Karma : Yoga vahi, Rsayana, Vrishya, Balya, Vayastambhana,
Pustikarak, Deepana, Agnivardhaka, Deha sidhikara, Loha sidhikara,
Shodhana, Ropana, Krimighna.
Dosha Prabhava: Tridoshagna
Vyadhi Prabhava: Vata roga, Valipalitha, Jara roga, Sarva Akshi roga, Krimi,
Kusta, Sarva roga.
MERCURY
Mercury is a silvery white metal, liquid at room temperature with high (13.6)
density. It is divisible into spherical globules, mobile, without having any odour /
taste, cold to touch, slowly volatizing at ordinary temperature. Low melting and
boiling point is due to large atomic size.
It is a soft metal, three times heavier than water. It forms amalgamation with
silver, platinum etc. Hg slowly oxidizes.
2Hg + O2 Heat 2 HgO.
General Description36:
Atomic Number : 80
Atomic Weight : 200.61
Atomic Volume : 14.8CC
Atomic Radius : 1.57 eg
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Ionic Radius (+2) : 1.10
Relative Atomic Mass : 200.50 gm/mole
Specific Gravity : 13.55
Melting point : 39.80C
Boiling point : 3570C
Occurrence and distribution:
Small quantities of mercury occur in native form but chiefly it occurs as
sulphide (cinnabar). It is found chiefly in Spain and Italy. It is also found as calomel
(Hg2cl2), Metacinnabar (HgS), Tiemannite (HgS), Montroydite (HgO) and also as
amalgums of Gold and Tellurium in small quantities.
Absorption, distribution and excretion37:
As the chemical form of the metal varies, its absorption, distribution and
Excretion of mercury also varies. In presence of O2 and Cl2 in the gastric contents, it
may dissolve to cause mild catharsis. Exertion of mercury immediately after
absorption is mainly through the kidney and colon and to a lesser extent via bile and
saliva. Small amounts are also excreted in volatile elemental form through both lungs
and skin. Most of Hg is excreted within 6 days after administration but traces may be
detected for months, even year’s urinary excretion is slow at first but accelerates later.
Fecal excretion is 8%, which is due to mucosal sloughing mainly as methyl mercury,
but bacterial flora converts about 50% to inorganic mercury.
Mode of Action38:
Most salts of mercury are absorbed slowly from the intact mucous membrane
of the elementary tract and produce their systemic effect. The sulphide ion is very
inert and it is clear that unless and until the salts are dissociated into its constituent’s
ions, mercury will not be able to exert its influence on the body tissues. Sulphides of
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mercury are not used in any of the pharmacopoeias of western countries as it is
considered to be devoid of therapeutic activity. The other mercurial salts after
absorption are excreted into caecum and colon as sulphides and in this form mercury
is found in the feaces.
When taken into the system it continues with acids and fluids of the body. It is
then easily absorbed by the skin, the mucous membrane, lungs and stomach and
passes into blood as oxy albuminate in the stomach it is converted into double
chloride of sodium of mercury. It unites with the albuminous juices and is easily
absorbed.
Toleration37:
Age, sex and idiosyncrasy greatly modify the action of mercurials, children as
a rule bear mercury better than adults and males better than females.
Therapeutic uses37:
Used as antiseptics, preservatives, parasiticides, fungicides, diuretics inorganic salts.
Externally as antiseptics, mercury salts are used.
Its solution is used for disinfecting surgical and obstetric practice.
Blue ointment and calomel ointment are used to reduce itching in prurigo, pruritis,
psoriasis, lichen pityriasis of scalp and eczema.
As a stimulant and promoter of absorption liniment and various ointments such as
oleate, red precipitate, scoltts and red iodide are used for promoting the absorption of
inflammatory products as in chronic joint disease and periostitis.
Mercury is used in certain eye diseases like conjunctivitis, blepharitis and keratitis.
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GANDHAKA
Gandhaka is grouped under Uparasa varga by authors of different Rasa
classics.
It is next to parada and has been explained in Dhatu Karma and in the
preparation of various Rasaushadhis. It is an essential agent for the various processes
of Parada samskaras such as Murchana, Jarana, Bandhana etc. Mercurial preparations
without Gandhaka are considered to be more toxic.
History:
Before 1000 B.C. description about Gandhaka was mentioned in Charaka
Samhita for the relief of various disorders along with other drugs. With the evolution
of Rasa Shastra importance of Gandhaka was also increased.
Mythological origin39:
Mythologically Gandhaka is said to be the result of churning of Ksheerasagara and it
is originated along with Amruta.
Gandhaka is considered to be the Raja of Parvathi.
Vernacular names40:
Assami – Kiburit; Bengali – Gandhaka; English – Sulphur; Gujarati –
Gandhaka; Hindi – Gandhaka ; Marati – Gandhaka; Parsi – Gogid ; Kannada –
Gandhaka; Telugu – Gandhakamu.
Synonyms41:
Gandhapashana Pamari Kauragandha
Gandhi Bali
Rasa Gandhaka Atigandha
Sugandhika Kushtari
Gandha Daityendra
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Saugandhika Gandhamadana
Putigandha Keetaghna
Types of Gandhaka42:
Rasarnava explained three types of Gandhaka and remaining others explained
four types.
Table No.6, showing the Types of Gandhaka according to Rasa Classics.
Sl.No. Types RRS RA AP YR RPS R.Chu
1. Shukapichchanibha (Pita) + + + + + +
2. Sukla (Shweta) + + + + + +
3. Shuka Chunchanibha
Shukatunda (Rakta)
+ + + + + +
4. Krishna (Black) + - + + + +
Table No. 7 Types of Gandhaka, their qualities and uses42:
Sl. No. Types Quality Uses
1. Shukachunchanibham Sreshta Dhatuvada
2. Shukapichchanibham Madhyama Rasayana Karma
3. Shukla Adhama Loha Marana
4. Krishna Jara Mrutyu Nashana
Grahya Lakshanas of Gandhaka43
The colour of genuine Gandhaka should resemble that of the tail of parrot (yellow).
Smooth, Hard, Unctuous.
Such Gandhaka should be used for medicinal purpose Shresta Gandhaka
should be having the lusture of Kapikacchu beeja and Navaneeta (soft to touch).
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For Rasayanartha and Loha vadartha, it should be translucent like the fruits of
Amalaki (Amlasara Gandhaka).
For the present study Amlasara Gandhaka is used.
Pharmacological and therapeutic properties44 :
Rasa : Katu, Tikta, Kashaya
Guna : Ushna, Sara Snigadha
Virya : Ushna
Vipaka : Madhura (R.C.), Katu (R.R.S; A.P)
Karma : Deepana, Pachana, Vishahara, Jantughna,
Rasayana, Bala Veerya Vardhaka, Jantu, Kandu, Visarpahara.
Dosha Prabhava : Kaphavatahara, pittavardhaka.
Vyadhi Prabhava: Garavishahara, Kshudra Kushta hara, Kasa,
Shwasa, Agnivardhaka, Rasayana, Dadruhara.
Doshas of Gandhaka45,:
According to Rasa classics, Gandhaka consists two types of Doshas:
Shila Churna Visha
(Physical impurities like clay, sand etc.) (Chemical impurities like arsenical, lead etc.)
Gandhaka should be purified before administering internally other wise it will
produce the disease like Kushta, Bhrama, Klama, Paithika Roga, Balakshaya,
Shukrakshaya, Veeryahani and Kandu.
Methods of Gandhaka Shodhana46:
Gandhaka is taken in darvi with equal amount of cow’s ghee and melted on Mrudu
agni. This liquefied Gandhaka is poured into another vessel, which contains cow’s
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milk through a cloth tied over the mouth of vessel. After that it is taken out and
washed with hot water.
Gandhaka is melted and poured into a vessel containing Bhringaraja Swarasa and
boiled for some time and this process is repeated for seven times.
Through urdhwa patana vidhi by using Damaru yantra Gandhaka can be purified.
In the present study, the following procedure has been adopted for Gandhaka
Shodhana46 a.
Gandhaka is taken in darvi with equal amount of cow’s ghee and melted on
Mrudu agni. This liquefied Gandhaka is poured into another vessel, which contains
cow’s milk through a cloth tied over the mouth of vessel. After that it is taken out and
washed with hot water
Effect of Shodhana on Gandhaka47:
By filtering through the cloth small pieces of stone and sand will be removed.
Some toxins may adhere to the ghee and hence will be removed.
Some of the toxins may mix with milk and thus the Gandhaka may be relieved from
impurities.
Dose of Gandhaka48
One to Eight Ratti.
Patya – Apthya49
Mamsa Bhakshana of wild animals and birds, cow’s milk, Ghee and Rice are advised.
Kshara, Amla, Atilavana, Katu, Vidahi and Stree Sevana Should be avoided.
Gandhaka Yogas
Kajjali Gandhaka Rasayana
Rasa Parpati Makaradhwaja
Rasa Sindhura Samirapannaga Rasa
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GANDHAKA MODERN VIEW
SULPHUR50 : The name sulphur is derived from the sanskrit word “Sulveret”
through the latin sulphurium.
History :
The ancients probably, due to its frequent occurrence in free state know sulphur.
Aryans, Greeks, Romans and Indians used it for fumigation and as medicine. The
Bible refers to be as “Brimstone” meaning “Burvaing Stone” Antony lavoiser placed
it among the elements in 1777, which was regarded as “principle” of fire”. It is
estimated as the Ninth most abundant element in the universe.
Basic information of sulphur
Name : Sulphur
Symbol : S
Atomic Number : 16
Atomic Mass : 32.06 Am
Melting point : 112.80C
Boiling point : 444.60C
Number of protons / Electrons: 16
Number of neutrons : 16
Classification : Non Metal
Crystal structure : Orthorhombic
Colour : Yellow
British Name : Sulphur
IUPAC Name : Sulfur
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Occurrence:
Sulphur is distributed in nature both in free and combined form. The sulphur is
found in volcanic regions in sicily. Approximately 0.06% of earth ‘s crust contains
sulphur. Pure sulphur contains traces of selenium, Tellurium and Arsenic some times
mixed with bitumen and clay.
Three are important minerals and compounds containing sulphur such as:
Sulphides: Zinc Blend (ZnS)
Galena (Pbs) S
Copper pyrites (CuFes2)
Cinnabar (HgS)
Iron Pyrites (FeS) S
Sulphate: Gypsum (CaSo4 2H2o)
Barites (BaSo4)
Epsom Salt (Mg So4 7H2o)
Ferrous Sulphate (FeSo4 7H2o)
Traces of sulphur occur as H2S in volcanic gases, organic substance as eggs, proteins,
garlic, mustard, onion, hair and wool. It is an essential non-metal and is a minor
constituent of fats, body fluids and skeletal muscles.
Appearance of sulphur in solid and liquid from
Solid : Rhombic and Monoclinic
Liquid : Aλ and Sμ (Amorphous Sulphur)
Therapeutic use51:
Sulphur has bitter astringent taste with a Characteristic strong smell.
It increases bile secretion, acts as laxative, alternative and diuretic.
It stimulates secreting organs like skin, bronchial mucus membrane.
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In larger doses it acts as purgative.
Sulphur is useful in cough, Asthma, General debility, Enlargement of spleen, chronic
fevers etc.,
Biological importance of sulphur52:
Sulphur makes up 0.25% of our body weight, meaning that an average adult human
body contains around 170 gms of sulphur, of which most occurs in the amino acids,
cysteine, cystine, and methionine.
Sulphur is involved in the formation of bile acids, which are essential for fat digestion
and absorption. It also helps to keep skin, hair and nails healthy.
Deficiency of sulphur is linked to the skin disorder eczema and also imperfect
development of hairs and nails.
Sulphur containing foods are vegetables (Radishes, Carrots, Cabbage, Milk Products
(Cheese), seafood and meat protein.
NIMBU53.
It is important dravya of Amla varga. In Rasa classics, it is explained for Shodhana
and marana of various metals and minerals.
Latin Name: Citrus accida Family: Rutaceae
Synonyms54:
Amlajambira Jantumari Amlasara
Nimbuka Dantaghna Rochana
Jambeera Shodhana
Description:55
Leaflets elliptic, oblong, racemes, short, flower small, petals usually four.
Fruits usually small, globose or ovoid, thick or thin. Pulp pale, very acidic.
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Useful parts:
Phala, twak and patra
Fruit juice of Nimbu contains citric acid 10%, phosphoric acid 47%, sugar 10.9%,
cellulose, vitamin A, vitamin C, citrine 76%, citrol 7.8% and Sulphric acid.
Distribution:
It is available throughout India.
Pharmacological and therapeutic properties:56
Rasa : Amla
Guna : Guru, Tikshna
Virya : Ushna
Vipaka : Madhura
Karma : Deepana,Rochaka,Anulomana, Pachaka, Krimighna
Dosha : Kaphavata Shamaka, Pittavardhaka
Vyadhi Prabhava : AgniMandya,Trishna,Udarashoola,Chardi,Aruchi, Vibandha,
Kasa Shwasa and Krimiroga.
For the present study Nimbu Swarasa was used for the Shodhana of Hingula.
HARIDRA57
It is a auspicious drug mentioned in all the Brihatrayee as a aghroushadhi for
prameha.it is classified under haritakyadiyarga.
BOTANICAL NAME: Curcuma longa.
Family: scitaminaceae.
Synonym:58
Haridra Haridrangani Swarnavarna vishaghni nisha
Vishaghni Mangalya Laxmi shobhana kanchani
Varavarnini Bhadra Shama jayanthika peeta
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Description: 59
A tall herb. Rootstoack large, ovoid, with sessile cylindric tubers orange coloured
inside.
Leaves very large, in tufts up to 1.2 meters or more long, oblong-lanceolate, tapering
to the base. Flowering in autumnal spikes, 10-15 cm long.
Useful parts: rhizome.
Distribution:
It is cultivated through the tropical and other regions in India.
Chemical constituents:
Rhizome contains a volatile oil 1%, an active principle curcumin, yellow colouring
matter and turmeric oil of specific odour and taste.
Pharmacological and therapeutic properties 60:
Rasa : Katu, Tikta,
Guna : ruksha, laghu.
Virya : Ushna
Vipaka : Katu
Doshakarma : kaphavatashamaka, pittarechaka-shamaka.
GODUGDHA61
Acharya charaka explained Godugdha under Gorasa varga. It is much appreciated for
the therapeutic purpose.
Synonyms:
It includes Ksheera, Gavya, Gavyadugdha, Dugdha, Payasa, Dhenudbhava
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Qualities of Godugdha:
It is having the qualities of Swadu, Sheeta, Mrudu, Snigdha, Bahala,
Slakshana, Pichchila, Guru, Manda and Prasanna
Contents of freshly drawn milk:
Water : 87% Phosphorus : 0.1%
Total Solids : 13% Sodium : 0.15%
Fat : 3.68% Iron : 1-2PP.M
Total proteins : 3.39 Citric acid : 0.2%
Sugar : 4.94 Calcium : 0.72%
Indications of Godugdha:
Godugdha is indicated in Kasa, Shwasa, Raktapitta, Trishna, Pandu,
Amlapitta, Shosha etc.
In the present work, godugdha was used for gandhaka shodhana,
GHRITA62
Ghee is one among the sneha Dravya. Ghrita, taila, vasa and majja are
considered as the best among all the snehana Dravyas.
Out of these four sneha Dravyas, Ghrita is considered as far superior owing to
its special attribute “samskarasya Anuvartanat”.
Synonyms:
Ghrita, Sarpi, Havisha, Gohavi.
Pharmacological Properties:
Rasa – Madhura.
Guna – Guru.
Veerya – Sheeta.
Vipaka – Madhura.
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Contents of Ghrita:
Unsaponifiable matter (Soluble in fat).
Triglycerides
Phospholipids
Vit D and K
Minerals like calcium, Magnesium, Copper, iron in traces.
Qualities of Ghrita:
Promoter of memory, intelligence
Improves digestion and metabolism
Best Aphrodisiac
It is refrigerant and emollient
Clarifies voice and complexion
In present study ghrita is used for the Shodana of Gandhaka.
KHALVI RASAYANA63
Khalvi Rasayanas comes under murcchna variety intended to render the chapalatva
and durgrahatva of Parada and potentiating it. It is one such category of Kajjali, where
in phytomedicines are triturated and pharmacologically has various advantages.
The specialty of Khalvi Rasayana lies in binding different varieties of drugs
into a single molecular form and there by minimizing the dose. Khalvee rasayana
impose proper particle size to drug, proper mixing occurs and gives the proper form to
the final product.
KAJJALI
Kajjali is a Sagandha, Niragni parada yoga. The bandha involved in this
preparation is Kajjali Bandha, where purified mercury and sulphur are intimately
mixed in definite proportion, to get a black powder called Kajjali.
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Among all Khalvi Rasayans Kajjali is having prime importance, as it forms
base to many mercurial preparations.
Definition:64
“ Dhatubirgandhakadyascha Nirdravaihi Mardita rasaha||
Sushlakshna Kajjalabhaso Kajjali Ityabhidheeyate||”
Shuddha Parada and Shuddha Gandhaka alone or in combination, with other uparasa
and different dhatus is mixed and triturated without adding any liquid to any powder.
This is called Kajjali. It should be free from any shining particles.
Any powdered pre-product that which is filled into Kupi should be smooth i.e., which
is having Slakshantva and sukshmatva like Kajjala is considered as Kajjali.
Proportion of Dravyas in Kajjali65
Parada, Gandhaka, Kajjali is used in many of the Yogas.
It forms the base for many of Kupi Pakwa rasayana, Parpati Kalpana and pottali
Kalpana. Accordingly the proportion of Parada, Gandhaka or any other dhatu varies
from one Yoga to another.
It is mentioned that Gandhaka can be taken in the preparation of ¼ th, ½, equal,
double, triple etc., to that of Parada.
In the present study Kajjali is prepared by adding equal amount of Parada and Shudda
Gandhaka. Trituration was done till all the Kajjali tests are positive.
Different forms of adding Dhatus to Parada
In the following pattern, other dhatus are added to Parada in the preparation of Kajjali.
If Swarna, Rajata etc., this should be in the form of fine leaves.
If Naga, Vanga etc., dhatus are to be added, these dhatus should be melted and then
added for trituration.
In case of Loha, tamra etc., its bhasmas should be mixed
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Tests of Kajjali66:
Krishna Varnata : Blackish colour
Slakshntva : Smooth to touch
Sukshmtva : Subtleness like anjana
Rekha purnatva : Settles in between fine lines of finger
Nischandratva : Lusterless a pinch of Kajjali is taken and rubbed with water.
This mixture when exposed to sun, should show absence of any shining particles
Uses of Kajjali67:
It is Tridosha hara and aphrodisiac.
It is used as sahapana and Anupana to eradicate many disorders.
Vatsanabha:
Botanical name – Aconitum ferox wall.
Family – ranunculanceae.
Introduction: 68,69
Vatsnabha is well known to the Ayurvedic pharmacopeia since long ago. We get
reference in Charaka samhita and Sushruta samhita Charaka samihita classified under
sthavara visha and Sushruta classified under Kandavisha and also explain its effects.
Sharangdhara and Bhavamishra mentioned Vatsnabha in their texts, along with almost
all Nighantus. Though Dhanwantari nighantu posses description of Vatsnabha,
synonyms and properties, most of the texts/Nighantus made little mentioning. The
utility of Vatsnabha has considerably increased after the development of Rasashastra.
Rasataranginikara classified it under visha.
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The term aconite refers to the genus aconitum of which there are several
species. The name may be originated form the Greek aconoits (meaning without
struggle or without dust) or from the Greek city acona where naturalist in the third
century once identified plant. Other sources suggest that the name comes from the
bill of aconites.
Aconites is Greek word meaning arrows coated with the poison and used for hunting
the animals. Aconitum if of two varieties viz poisonous & non poisonous.
Among the poisonous varities both aconitum ferox and aconitum chasmanthum are
used as vatsanabha in India.
Synonyms:70
Table No. 08. Showing the synonyms of Vatsnabha
Sl Names
D.N R.N R.T. B.P
01 Vatsnabha + + + -
02 Amruta + + + -
03 Visha + + + +
04 Ugra + + - -
05 Maha oushadha + + - -
06 Garalam + + - +
07 Marana + + - -
08 Naga + + - -
09 Stokakam + + - -
10 Pranaharaka + + - -
11 Sthavaradyam + + - -
12 Kshweda - - + +
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Vernacular Names: 71
01 Sanskrit - Visha, Vatsnabha
02 Hindi - Bisha,Mithazahar
03 English - Indian aconite
04 Bengali - Katbish or Mitha visha
05 Telugu - Vasanubhi
06 Kannada - Vatsanabi
07 Gujarathi - Vachanag
08 Marathi - Vacha nag
09 Assam - Visha
10 Malyalama - Vatsanabi
11 Malyalama - Vatsanabi
12 Punjabi - Mohari
13 Arab - Bish
14 Parse - Bishmag
Classical Categorization:
Charaka Samihita - Stavara Visha
Sushruta Samhita - Kanda visha
Dhanawanatari nighantu - Misraka varga
Raja nighantu - Misraka varga
Bhavaprakasha nighantu - Dhatvadi varga
Rasatargini - Visha
Modern Toxicological Categorization:
Cardiac poison.
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Different verities of Vatsnabha (BHEDA)
Table No. 09. Showing the Vatsnabha bheda According to Yogaratnakara 72
Sl. No. Bheda Varna Guna
01. Brhmana Pandu Rasayana
02. Kshatriya Rakta Deha pustikara
03 Vaishya Peeta Kustaghna
04. Shudra Krishna Dhatukarma
According to Rasatarangani 73
01. Krishna
02. Kapisha
03 Pandu
Kapisha is better than Krishna; Pandu is better than Kapisha Pandu is considered best
for therapeutic uses.
According to Ayurveda Prakasha 74
01. Shukla
02. Krishna
Identification 75
Rasavagbhata mentioned certain characteristics for identification
1. Vatsanabha Panduravarana
2. Roots resembles, navel of calf
3. They are Stoola snigdha, Guru, Nava,
According to Bhavaprakash:
Leaves resembles sindhuvara and roots resemble navel of calf.
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Botanical description: 76
It is a perennial herb
Root – Paired, daughter, tuber ovoid oblong to ellipsoid, 2.5.4 cm long, about
1-1-5 cm thick, with fill form root fibers, dark, brown externally, yellowish on
fracture, another tuber much shrunk and wrinkled with more numerous root
fibers.
Stem – Erect, with or without a slender, hypogynous base, simple, 40-90 cm
high covered with short spreading yellow hairs in the upper part and glabrous
below.
Leaves – Scattered, distant, glabrous, petioles, slender up to 25 cm, blade or
bicedar-cordate to remiform in out line with rather wide sinus. Planately 5-
lobeal.
Inflorescence: - Peduncle straight, bearing flowers on both sides, flowers pale,
blue, brown in a dense, terminal raceme, 10-25 cm long, helmet, volatile with
short shared beak, resembling a pea flower.
Fruit – Carpels 5, tomentose, follicles oblong, 15-20mm long and 4-5 mm
broad, seeds obovoid to obpyramidal, 2,6-3 mm long, winged along with the
raphe.
Distribution – Grows solid in the alpine Himalayas, Kashmir at an attitude of
3,600 m, alpine Himalayas of Nepal.
Chemical Constituents – Roots contains toxic alkaloids, pseudo aconitine along
with bikha aconitine, chasmacontine, chahnaconitine, indacontine (Loydia
1972,35, 55) Veratroy1)pseudoacontine are diacety1 psedioacontine(manske and
Rodrgo) 1979).
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Two new alkaloids 2 – (11+) quionolinome and 3,4dihydro – 6 – hydroxy – 2 –
(11+) quoinolone (Nat. prod. Lett.) 1993, 227,chem, abortr. 1994, 121, 175,
172b).
A new diterpenoid alkaloid – 14- 0 – acetyl senbasimet, vakagnavime, chasma
contine, crassican line A. flconericine, senbusine B. isoltaltizocline and aconine
are reported (phyto chem, 1994, 36, 1527).
Four lipoalkalaoids Viz veraatrophylpseudaconine, auisolyona- contine,
benzoylida aconine and veratroy bikkaconine are also report (J. Nat prod 1994,
57, 105)
Need of Vatsnabha Shodhana- 77
Ashodhita Vatsnabha causes daha,moha, hirtagata rodha, to avoid these doshas or
vikaras it should undergo shodhana process
Vatsnabha shodhana -78
Table No. 10, showing the Vatsanabha shodhana according to different authors.
Sl.
No.
Shodhana process R. T. R. A. D.G.V Y. R. R. J. N
1 Kept in cow’s urine in
strong sunlight for 3 days
+ + - - +
2 Swedana in Ajadugdha
for 1 yama
+ + - - -
3 Swedana in Surabhi
payas (cows milk) for 1
or 2 yama
+ + - + -
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4 Kept in cow’s urine for 3
days then Swedana in a
cow’s milk or goat’s milk
for 3 hrs
- - + + -
5 Swedana in dolayantra
containing Triphala
kashaya and Aja ksheera
- - - - +
6 Swedana in dolayantra
containing cow’s urine.
- - - - +
Gunakarma 79 –
Rasa - Madhura
Guna - Ushna
Veerya - Ushna
Vipaka - Madhura
Dosha Karmarma - Vata-kapha Shamaka
Dhanwantari nighantu classified under Mishrakadivarga. Madhura rasa,ushna
guna, vatakaphahara, it subsides kanthashoola, sannipatagna, pitta samshodhini80
Rajanighantu81 classified under pippalyadi varga. Atimadhura, ushna,
vatakaphahara. It subsides Kantaruja, sannipataghna, pitta santappa Karaka.
According to Rastarangin82 , vatsanabha is katu, tikta,kashaya rass,ushna guna
and yogavahi. It is rasayana, tridoshahara particularly vata-kaphahara. It increases
agni. It subsides sheeta and brumhana and bala vardhaka. It subsides agnimandya
rogas, pleeha roga,vatarakta, shwasa, kasa, grahani, gulma, pandu, jwara and amavata.
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It relieves timira, naktandhyata, netrabhishyanda, netrashotha, karna shoola, gudaroga
and kati vedana. Application of bhahya lepa subsides the aku, vrushika, sarpavisha83
Diaphoretic, diuretic, antiperiodic, anodyne and antidiabetic, antiphlogistic,
antipyretic in small does. In large does it is virulent poison, narcotic and powerful
sedative. It reduces the frequently and tension of the pulse and paralysis the
respiratory center.
Part used - Dried tuberous root.
Dose84 - 1/10th ratti to 1/8th ratti
Visha prayoga Nishedha 85
Balaka, atyantavridhha, garbhavati, rugna, atikshinashareera, rajayakshma
laxanayaukta avasta, krodhi, atibhranti, durbalavastha in less quantity and short
duration with precautions
Toxic effects and antidotes;86
Sushruta clearly documented the toxic efforts of Vatsnabha viz Grivastambha
and peeeta vit, mutra, netra.
Antidotes –
Accidental poisoning or over dosage with aconite may produce the
toxicsymptoms. Different antidotes have been mentioned for the management.
Gogrutha is considered as one of the best antidotes for visha.
Tankana (Borax) is considered to be the main antidote87. It may be
administrated along with Ghee. Arjuna bark is mixed with Honey and Ghee may be
another alternative antidote88.
Aconite poisoning and its management in toxicology89.
The symptoms of poisoning occur immediately or within a few mines after
consumption or root. First burning sensation is experienced from the mouth to
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stomach followed by tingling and numbness in the mouth, tongue and pharynx. This
is followed by salivation, Nausea, Vomiting and diarrhea. Later dryness of mouth and
polydypsia is developed and the patient will be unable to swallow
Other symptoms include headache, giddiness, pallor, profuse, sweating,
subnormal temperature weakness of limbs and inability to stand or walk. Twitching
of muscles, pain, and cramps and convulsions may occur.
The pupils contract and dilate alternately but remain dilated at the later stage.
Dimness of vision and diplopia may be caused. The pulse becomes slow, feeble and
irregular. Blood pressure will be low and the patient complains of breathlessness.
The mental conduction remains normal but there may be hallucination. Death finally
occurs either due to paralysis of heart or respiratory centers or even both.
Fatal Dose - 1-2 grams of root OR 4-6 mg of acontine
Fatal period - 3 –6 hours.
Treatment – Gastric levage with warm water and weak solution of potassium
permanganate or with a solution of iodine in potassium iodide or with tannic acid or
strong coffee or strong tea to precipitate the alkaloids.
1. Powdered charcoal to diminish solubility.
2. Atropine –0.5 – 1 mg is useful.
3. Strychnine, artificial respiration, application of heat etc,may also be useful.
4. Symptomatic treatment
Vishishta yogas – 1. Ramabanarasa
2. Hinguleshwara rasa
3. Tribhuvankirti rasa
4. Kaphaketu rasa
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5. Swachanddha Bhairava rasa (Dwitiya)
Gomutra
Gomutra is used as a medicine since olden days. We get the reference about it
in brihatrayees –Charaka considered, it as one of the ashta mutra varga dravya.
Synonyms90 – Gomutra, Gojala, Goambu, Gomashanda, Godrava.
Vernacular names-
Sanskrit - Gomutra
Hindi - Gomutra
English - Cow’s urine
Kannada - Gomutra
Properties:91
Rasa - Katu
Guna - Teekshna, Ushna,Kshara
Dosha - Kapha –vata shamaka, pitta janaka
Karma - Agni deepaka, medhya, shoolahara, gulma, anaha, it is
used in viechanna karma and asthapana basti
Charakacharya92 quotes that Gomutra has madhura rasa, dosha nashaka and
krimi and Kanduhara. By abhyantarapana it subsides doshajanya udara roga .
Nadakarni93 in his material medica explains Gomutra contains ammonia in a
concentrated form and is much used in both internal and external purpose. Gomutra
is a laxative, duretic and used in preparation of various medicines. Eg. Poonaranava
mandura. It is also recommended by Chakradatta as a anupana for eranda taila given
as virechana.
It is used as externally in the purification and roasting of various metals and
preparation of oils, decoctions etc
In the present study gomutra is used for vatsanabha shodhana.
Pippali:
Botanical name - Piper longum linn.
Family - Piperacae
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Introduction
In Atharvana veda Pippali is mentioned as rasayana. Kshipta bheshaj,
atividdha abheshaji and vati krita bheshaji. Pippali is drug which is widely used in
Ayurvedic formulations Charaka and Sushruta have extensively quoted pippali among
dashemaniya gana
Gana: 94
Charaka : Deepaniya, Kanthya, Asthapanopaga, Shirovirechanopaga,
sheetaprashamana, Shula-prashamana, Kasahara, Hikkanigrahana,
Triptighna.
Sushruta : Pippalyadi, Amalakyadi.
Astanga sangrahakara : Pippalyadi gana and Nyagrodhadi gana.
Vernacular names:95
01 Sanskrit - Pippali
02. Hindi - Pipala
03 English - Piper.
04 Telagu - Pippali
05 Tamil - Tippali
06 Bengali - Pipali
07 Marathi - Hippali
08 Malyalam - Tippali
09 Punjabi - Moghaum
10 Gujarati - Pipali
11 Kannada - Hippali
12 Parse - Pipli
13 Burma - Peikchin
14 Arb - Darfifil
15 Barma - Pekchin
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Table No. 11, showing synonyms of Pippali 96
Synonyms B. P. K. N. N. A. R. N. D. N. Ma. Ni.
Magadhi + + + + + +
Shoundi + + - + + +
Vaidehi + + + + - +
Chapala + + + + + +
Kana + + + + + -
Krishna + + + + + -
Upakulya + + - + + +
Teekshna tandula + + + + +
Magadha - - - - - +
Vishwa - - - - - +
Kola + - - + + -
Kukara - - - + - -
Katu beeja - - - + - -
Korangi - - - + - -
Tikta tandula - - - + - -
Magadodbhava - - - + - -
Ushana - - - + + -
Bheda97
It is of 4 types –
Pippali
Gajapippali
Simhali
Vana pippali
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Botanical Description 98
An aromatic slender, Climber
01 Stem - Cripping, jointed, attached to other plants while climbing,
02 Leaves
- 5.9 cm x 3.5 cm , subacute, entire, glabrous, cordate at the
case
03 Flower - In pendular spikes, straight
A. Male - Larger and slender
B. Female - 1.3 – 2.5 cm x 4.5 cm in diameter
04 Fruit - Yellowish orange ovoid, sunk in flesh spikes fruits in
rainy season and fruit in autumn
05 Distribution - Found in the hotter parts of India, form central Himalaya
to Assam and Mikir hills. Also found in forests of
western ghats form Konkan to Kerala.
06. Chemical
Construction
: Essential oil, mono and sesquitrpenes, Caryophylience,
piprine, piper longumine, piper longuminne, piper
nonaline, piper,undecalindine, pipercide, seasmin, B-
sitosterol, four aristolactan, five 4,5 – dioxporphions.
Properties:99
Rasa - Katu
Guna - Laghu, Snigdha, teekshna, (Ardha guru)
Veerya - Anushna sheeta veerya
Vipaka - Madhura
Karma - Vata –shleshmahara
Useful parts - Fruit, root
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Dhanwantari nighantu, includes under shatapushpadi varga, pippali have the
properties of katu rasa, madhura vipaka, sheeta veerya, snigdha guna, tridoshahara. It
is rasayana and subsides jwara, trishna, udara roga, krimi, amadosha .
Raja nighantukara includes under pippalyadi varga, Pippali have the properties
of snidhaushna guna, katu tikta rasa. It is vrishya, deepana and subsides jwara, kasa,
shwasa, kaphahara.
Bhavaprakasha nighantukara includes pippali under haritakyadi varg. Pippali
have the deepana, vrishya, madhura vipaka and rasayani. Anushana sheeta veerya,
katu rasa, snigdha laghu, vata shleshmahara. It subsides shwasa, kasa, udara, jwara,
kushta, meha, gulma, arsha, pleeha shoola, amavata. Ardra pippali is kaphaprada,
snigdha sheetala, madhura and and guru.
Madanapala nighantukara has included pippali under shunthyadi varga.
Pippali has properties of deepana, vrushya, madhura paka, rasayani, atyushna, katu
rasa, snighdha laghu, kaphahara, pitta rechani. It subsides swasa, udara, jwara.
Kaiyadeva nighantukara includes pippali under oushadhi varga. Ardra pippali
have the property of sheeta, guru, madhura, snighdha, ushna, kaphaprada. Shushka
pippali is laghu madhura paka, snigdha ushna, katu rasa, kapha vatahara ruchya, sara,
vrishya, rasayani, deepani, pachani. It subsides kapha, gulma, arsha, meha, pleeha,
jwara, udara.
Infusion is stimulant, carminative and alternative tonic more powerful than
black piper, also aphrodisiac, diuretic, vermifuge and emmengogue. Externally
rubefacient.
Piper longumin or piperine shows immuno-modulatory and antitumour
activity piperine also shows cytotoxic towards DLA and EAC cells at concentration of
250 micro gm/ml. Administration of piper longum or piperine increase the total WBC
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count. Bone marrow cellularity and alpha esterase positive cells were also increase by
the administration of piper longum extract piperine.
Pipper longum or piperine shows to posses bioavilability enchancing activity
with various structurally and therapeutically diverse drugs. It may be hypothesized
that, piperine bioavailability enchancing property may be attributed to increased
absorption, which may be due to alteration in membrane lipid dynamics and change in
conformation of enzymes in the intestine.
Piper longum acts as antioxidant and is predominant in catalytic activity. It
contains vitamin E and A. The antioxidant component of piper species constitutes a
very efficient system in scavenging a wide variety of reactive oxygen species.
Dosage-
Choorna- 0.5-1 gm.
Vishishta Yoga-
Pippali ghrita
Pippalyasava
Vyoshadi vati
Pippalyadi leha
Yakritapippali yoga
Vardhamana pippali
JATIKOSHA:
Jatikosha is mentioned in all the Brahatrayees for therapeutics purpose.
Family : Myristicaceae
Botanical name : Myristica fragrans Houtt.
VERNACULAR NAMES:100
Hindi : Javithri
English : Mace tree
Telugu : Jathipatri
Tamil : Adipalam
Bengali : Jaitri
Kannada : Jati patri
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BOTANICAL DESCRIPTION:101
Evergreen aromatic tree, 9-12 mt high
Bark : Greyish – black.
Leaves : Coriaceous, elliptic, deep green above and grayish beneath.
Redish-grey when ripe.
Flowers : Creamy – yellow, brangrant.
Fruits : Globose or broadly pyri form 6-9 cm long, pear shaped,
glabrous, often drooping , yellow; pericap fleshy, 1.25 cm thick,
splitting into two halves when mature.
Mace : It is epicarp of fruit, fleshy reddish colour & lacinate.
Seeds : Arillate, albuminous, broadly avoid, with a shell- like purplish brown
Distribution : Native of Moluccas. Grown in kerala, Karnataka, Nilgiris and
W. Bengal.
Chemical Constituents:
Mace yields volatile oil, 8-17% fixed oil, resin, fat, sugar and mucilaginous
matter. Mace also yields an yellowish aromatic oil which contains a chemical
substance macene.
Properties-102
Rasa : Tikta, katu, madhura
Guna : Laghu
Virya : Usna.
Vipaka : Katu.
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SYNONYM: 103
Table No.12, Showing synonyms of Jati kosha
S.l.no Synonyms B.P.N
K.N D.N M.P.N R.N
1 Jatikosha - + + - +
2 Jatipatri + + + + +
3 Javitri + - - - -
4 Patrika - - - - +
5 Sumana - + + - +
6 Malatipatrika - + + + +
7 Jatiparna - - - + -
8 Soumanasayani - + - - -
9 Malanashini - - + - -
Table No.13, Showing actions of Jatikosha104
S.l.no Actions B.P.N
KN DN RN
1 Ruchi + + + -
2 Varnya + + + -
3 Vaktravyaishyadya
janani
- - + +
4 Jadyadoshanirkuth - - - +
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Indications-105
Table No.14, Showing Indications of JatiKosha
S.l.no Indications B.P.N MPN
KN DN RN
1 Kapha + + + - +
2 Kasa + - + - -
3 Vami + - + - -
4 Swasa + - + - -
5 Trushna + - + - -
6 Krimi + + + - -
7 Visha + + + + -
8 Kaya shanty - - - + -
Review on various Pharmaceutical Process Adopted in the present study.
Definition 106:
The process, which eliminates the blemishes, is called Shodhana.
Shodhana is a process intended for the removal of impurities in a substance by
implementing prescribed method like trituration etc with prescribed drugs.
Shodhana is the process meant for the lohas, Dhatus, Rasas etc., by subjecting
them to Swedana, Mardhana, Dhalana etc., which results in the detraction of
blemishes.
Advantages of Shodhana :
Eradicates visible and invisible impurities.
Reduces toxic effects.
Removes adulterants present in drug.
Makes hard matter brittle which helps in easy incineration.
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Enhances therapeutical properties
Suitable for further processing
In the present work, concept of Shodhana is adopted in following procedures:
Shodhana of Raw Hingula by means of mardana with Nimbu swarasa in Khalva
Yantra.
Shodana of Hingulota parada by triturating with haridra and Nimbu swarasa.
Shodhana of raw Gandhaka in dugdha and gritha.
Shodana of vatsanaba keeping it in Gomutra.
Yantras used in the present study
Khalva Yantra: 107
Khalva Yantra denotes mortar with pestle, made of varieties of stones of good
quality in different shapes and sizes.
Uses: It is used for Grinding, Rubbing, Triturating or mixing of drugs.
In the present study it was used for:
• To powder crude Hingula and Gandhaka
• Mardana of Hingula with Nimbu Swarasa.
• In the preparation of Kajjali.
Urdhwa Ptana Yantra: 108
• The Yantra is made with two earthen pots, where the upper pot is bigger than
the lower pot.
• The upper pot’s pristatala should be broad enough (i.e. 16 angulas) to
construct toyadhara.
• The mouth of the upper pot should be inserted into the mouth of lower pot in
such a way, that the same should reach upto the neck of the lower pot.
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• The joint of the appartus should be sealed air tight with the help of
multanimitti-smeared cloth or other sealing material.
• The lower pot contains the drug, which is subjected to sublimation, and the
outer part of the upper part has toyadhara, which felicitates the sublimation.
In the present work, urdwa Patana Yantra was used to extract parada from
Hingula.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 52
Methodology
METHODOLOGY
Methodology was studied mainly under two headings.
• Pharmaceutical study.
• Analytical study.
Pharmaceutical study:
This section deals with identification, selection and, processing of raw drugs
and preparation of Swacchanddha Bhairava Rasa (Dwitiya).
The very purpose of this branch of medicine is to provide safe and effective
medicine Ayurevda believes that, “Samskara” given to the drug will change the
quality of drug and also drug acts in different manner when mixed with other drugs.
Study design: This section includes three steps:
Step –01 : Identification and collection of raw drugs
Step-02 : Purification and processing of raw drugs
Step-03 : Preparation of Swacchanddha Bhairava Rasa (Dwitiya).
Step 01 - Identification and collection of raw drugs
Date of commencement: 05 – 01 - 06
Date of completion : 07 – 01 - 06
Proper identification, selection and collection of raw drugs are necessary for
Ayurvedic formulation, because without these things we cannot assure the quality of
our medicaments. So this section of the study deals with the same Swacchanddha
Bhairava Rasa (Dwitiya) contains the following ingredients:
• Shoditha Hingulotha
parada
• Shoditha Ghandhak
• Shoditha Vatsanabha
• Pippali
• Jathikosha
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 53
Methodology
Special request was made to the local herbo-mineral drug shop dealer to get
the particulars quality drugs and those were screened for classical grahya lakshanas
and those were certified by the concerned departments.
Step:02: Purification and processing of raw drugs:
Ayurveda has enlisted certain drugs, which will cause adverse effects or no
theapeutic effects if used in the impure state or may lead to complication. So proper
purification is necessary to counteract the probable adverse effects. This section deals
with the purification and processing of raw drugs.
Practical No. 01:
Title: Hingula shodhana.
Date of Commencement : 10 – 01 – 06.
Date of completion : 21 – 01 – 06
Reference: Rasatarangini, 9th chapter 16-17sloka
Materials required: Khalwa yantra
Drugs used:
a) Hingula - 200 gms
b) Nimbuswarasa - Q.S.
Table No. 15, showing the Results of Hingula Shobhana.
Ingredients
In quantity
Bhavana dravya in quantity Mardana in
hours
Results Remarks
Nimbu swarasa 100ml 6hours 205 gms Gain – 5 gms.
Nimbu swarasa 100ml 6 hours 212 gms Gain – 7 gms.
Hingula
Gms
200 Nimbu swarasa 100ml 6 ½ hours 218 gms Gain – 6 gms.
218 gms Nimbu swarasa 100ml 5 ½ hours 224 gms Gain – 6 gms.
224 gms Nimbu swarasa 100ml 6 ½ hours 229 gms Gain – 5 gms.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 54
Methodology
229 gms Nimbu swarasa 100ml 6 hours 235 gms Gain – 6 gms.
235 gms Nimbu swarasa 100ml 7 hours 242 gms Gain – 7 gms
Table No. 16, showing the quantity of Hingula before shodhana and after shodhana.
Draya Quantity of Hingula before
shodhana (in gms)
Quantity of Hingula after
shodhana (in gms)
Hingula 200 242
Method :
Hingula was taken in a Khalva yantra and powdered nicely, 100 ml of Nimbu swarsa
was added and mardana was done for 6 hours. This process repeated for seven times.
Observation:
While powdering Hingula, white shiny lines were seen.
After half an hour of mardana, shiny particles disappeared
After 25-35 minutes the colour turned to red
Precautions:
Intially mardana was done slowly to avoid the spillage of material.
When it attained semisolid consistency the mardana was carried out
continuously.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 55
Methodology
Practical No. 2: Hingulotha parada
Hingulotha Parada Nirmana (Extraction of Parada from Hingula)
Date of Commencement - 06 – 01 – 06
Date of completion - 20 – 01 – 06
Reference : Rasatarangini, 9th chapter 13 – 17 sloka.
Drugs used - Hingula -200gms, Nimbu Swarasa 60 ml.
Apparatus – Khalva Yantra, juice extractor, knife, Spatula, Urdwa patana yantra
(two equal sized mud pots), cloth, Multani Mitti, Agni Chulika, Cold Water, cotton
pad etc.,
Procedure:
200 gms shodita Hingula was taken in Khalva Yantra, then powdered, 60 ml of
nimbu swarasa was added and triturated uniformly at a rate of 30 strokes / min for
3 hours.
Then chakrikas were made. This was then allowed to dry in shade.
Weight of dried Hingula after Bhavana was 215 gm., prepared chakrikas were
placed in Urdwa patana yantra and another large sized earthen pot was inverted
over it.
Sandhibandhana was done with a cloth smeared with multani mitti and dried.
Such seven layers were applied after drying of the earlier one.
Urdhwa patana yantra was kept over the chullika, madhyamagni was given for 6
hrs and then Tivragni was given for 2 hrs.
After Swangasheeta, the next day sandhibandhana was carefully removed. 2 pots
were separated; soot with mercury globules was collected from the inner surface
of the upper pot by scraping carefully with a plastic spoon.
Parada was filtered through double fold cloth to get clear mercury.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 56
Methodology
Observations :
Table No. 17 Showing observations during Hingulotha Parada
Date Initial weight
of Hingula
Parada
extracted
Agni given in
hrs
Unburnt
Hingula left in
lower pot
215 gms 115 gms 8 hrs 15 gms
• The dark red colour powder of Hingula became brick red after adding the nimbu
Swarasa, and during the addition of nimbu swarasa to Hingula small bubbles and
white streaks appeared.
• The chakrikas were shade dried at room temperature 27o C with relative humidity
70%.
• The complete drying of chakrikas took nearly 48 hours.
• After drying chakrikas of Hingula appeared Sindura coloured (dark reddish) with
smooth surface and it was observed that weight of Hingula was increased after
drying of the chakrikas from 200 to 215 gms.
• The dried chakrikas were taken for the Hingulakrusta parada in urdhwapatana
yantra.
• After 2 hours of heating the bottom of the lower pot appeared red hot and at 2-½
hrs sulfurous smell was observed.
• After Swanga Sheeta when the Sandhi Bandhana was opened, the mercury
globules along with the soot were found in the central portion of upper pot and in
the lower pot about 15 gm of talasta dravya was obtained in the procedure.
• Some globules of mercury were also seen in the lower pot along with burnt
material.
• Mercury collected was Shining like a madhyana surya.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 57
Methodology
Precautions:
• Mardana procedure was done carefully to avoid spillage of material.
• Fresh nimbu swarasa was added.
• After it attains semi-solid consistency, mardana was done firmly and continuously
• Completely dried Hingula was kept in urdhvapatana yantra.
• Sandhi bandhana was done carefully to avoid over lapping of chakrikas in urdhva
patana Yantra. After each sandhibhandana, it was properly dried.
• While heating, upper pot was kept cool by frequently changing the wet pad over
the pot.
• Uniform and calculated heating was carried out.
• Water was not allowed to fall on Sandhi bandhana.
Results:
Weight of Chakrikas - 215 gms
Weight of Parada extracted - 115 gms
Loss of weight - 100 gms
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 58
Methodology
Practical No : 3
Name of the practical : Shodhana of Hingulotha Parada
Date of Commencement : 27 – 02 – 06
Date of Completion : 28 – 02 – 06
Reference : Rasatarangini, 5th chapter 40 – 41 sloka.
Time taken : 2 Days
Apparatus : Khalva Yantra
Drugs used : Hingulatha Parada 115gms
Haridra Churna–7gms,
Nimbu Swarasa– 10ml
Kanji – Q.S.
Procedure : 115gms of Hingulakrusta parada was taken into a porcelain mortar and 7
gms of Harida churna was added & triturated with nimbu swarasa for 2 days and
allowed for drying. After complete drying powder was collected and filtered through
the double folded cloth for 4 times and washed with Kanji.
Observations :
• Yellow haridra churna turned to brilliant green on trituration.
• Slowly parada turned into small droplets and mixed with haridra powder
completely.
• Paste was glittering in sunlight.
• On second day trituration was done by adding required quantity of nimbu swarsa
& triturated until the parada turns into fine particles and get homogenous with the
paste.
• Little quantity of parada along with haridra choorna got adhered to the mortar and
pestle.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 59
Methodology
• Finally the collected mercury was silvery white.
Precautions:
• Throughout the procedure spillage of the material from khalva yantra is avoided.
• Filtration should be carried out after drying of the mixture.
• Small quantity of mixture (20-30 gms) each time should be filtered through
double fold cotton cloth.
Table No. 18, Showing observations during Samanya Shodhana of Parada
Parada
Shodhana
Hingulaha
Parada
Haridra
Choorna
Nimbu
Swarasa Kanji
Shuddha
Parada
Loss
during
Shodhana
1 115gms 7gms 10 ml Q.S. 100 gms 15gms
Practical No. 4: Gandaka Shodhana
Title : Gandhaka Shodhana
Date of commencement : 09 – 03 – 06
Date of completion : 10 – 03 – 06
Reference : Rasatarangini, 8th chapter shloka7-11
Drugs used : Gandhaka100gms, godugdha300ml, gogritha 300gm
Method: gandhaka was made into powder.gritha was taken in a vessel and heated,to
this gandhak powder was added when gandhaka completely melted it was poured into
a vessel containing godugdha with mouth covered with cloth. solidified gandhaka was
taken out and washed with hot water. This procedure repeated for three times.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 60
Methodology
Observation
All mud particles and dusts, which were present in Gandhaka, were separated out
over the cloth during procedure.
Shodhita Gandhaka was of pale yellow coloured & shiny.
Shodhita Gandhaka was in pindakruthi
Precautions:
Agni should be mrudu.
After melting of grita powdered gandhaka should be added slowly.
Melted gandhaka should be poured immediately to the vessel containing milk covered
with cloth.
After each procedure gandhaka was washed with hot water.
Table No. 19, Showing the Quantity of Gandaka before & after sodhana
Draya Quantity of Gandaka
before sodhana (in gms)
Quantity of Gandaka after shodhana (in
gms)
Gandaka 100 82
Practical No.5: Kajjali Preparation:
Name of the practical : Preparation of Kajjali
Date of commencement : 13 – 03 – 06
Date of completion : 16 – 03 – 06
Reference : Rasatarangini, 6th chapter 110 – 111 sloka.
Drugs used : Shodhita Hingulotha Parada –60gms
Shodhita Gandhaka –60gms
Apparatus : Khalwa Yantra, Spatula.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 61
Methodology
Procedure:
• 60gms of Shoditha parada was put in Khalva, to this finely powdered Shodhita
Gandhaka was added and triturated.
• Trituration was done slowly with uniform speed till all the Kajjali lakshanas were
observed i.e. the whole mixture converts into a fine, black, smooth, lusterless
powder.
Observations:
• After 5 minutes of trituration bigger mercury globules were broken into smaller
globules.
• After 15 minutes of trituration mixture appeared blackish yellow coloured and
tailing of mercury was seen.
• After 25 minutes mixture appeared dull grey coloured with small shiny globules.
• After 40 minutes mixture appeared Portland cement coloured between which
yellow streaks were seen while triturating.
• No mercury globules were seen 1 hour of mardana. Shining was present, mixture
was Cairo dust colour.
• After 2 hours mixture appeared blackish Grey coloured.
• After 6 hours of trituration, mixture appeared blackish coloured. Shiny particles
were observed.
• After 18 hours mixture appeared black coloured. Tests of Kajjali i.e.,
Rekhapurnatva, varitaratva and Slakshnatva were absent.
• Mixture turned completely into soft, smooth black compound after 32 hours.
• After 42 hours Rekhapurnatva and Slakshnatva were observed in the compound.
• Varitaratwa and Unama were observed in mixture after 55 hours of Mardana
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 62
Methodology
• Little quantiity of Kajjali as put on fire and observed, it burns with fumes.
• After 65 hours, Kajjali was taken between thumb and index finger made wet then
rubbed and was exposed to sunlight, no mercury particles were observed.
• Shiny Kajjali flakes were seen adhered at the bottom of Khalwa Yantra.
• For better fineness and smoothness of Kajjali, Mardana was continued upto 72
hours.
• Average to & fro movements of peshani were 14-15 times/ minutes.
Table No. 20, Showing different phases of Kajjali during preparation.
Hours Observations
After 0 hours Parada + Gandhaka
After 15 minutes Tailing of Parada observed
After 25 minutes Grey colour with shiny globules
After 40 minutes Portland cement colour with yellow streaks
After 1 Hour Absence of Parada globules
After 2 Hours It turned to blackish Grey
After 6 Hours Blackish colour with shiny particles
After 18 Hours Test for Kajjali was absent
After 32 Hours It turned to black fine powder
After 42 Hours Attained Rakhapurnatva and Shlakshnatva
After 55 Hours Varitara and unama tests were positive
After 65 Hours Nishchandratva was observed
After 72 Hours Showed completion of Kajjali lakshanas.
Table No. 21, Showing Physical properties of Kajjali
Colour - Black Odour - Sulphurous
Form - Fine Powder Touch - Soft and Smooth
Taste - Tasteless Appearance - Anjana Sadrisha
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 63
Methodology
Precautions :
• Khalva Yantra should be clean and dry before starting the process.
• Shodhita Gandhaka was finely powdered, before adding to Shodita Parada.
• Mardana was done carefully and in uniform speed to avoid spillage.
• The pestle was moved entire length of Khalva Yantra in clockwise /Anti
Clockwise direction.
• Kajjali was collected after the completion of Lakshanas.
Results :
Quantity of Shuddha Parada - 60gms
Quantity of Shuddha Gandhaka - 60gms
Weight of Kajjali - 110gms
Loss of weight - 10gms
Practical No.6 VATSANABHA SHODHANA
Date of commencement : 27 – 03 – 06
Date of completion : 08 – 04 – 06
Reference : Rasa tarangini 24 / 77-78.
Equipments : Ulakalayantra, Vessel, Cloth etc.,
Ingredients and quantity:
Dravya Mana
Vatsanabha 250 gms
Gomootra Yathavashyaka
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 64
Methodology
Method of purification:
• 250 gms of roots of Vatsanabha were taken and made into small pieces
and washed well to remove the impurities (external).
• The pieces of Vatsanabha were tied in cloth and Pottali was prepared.
• This pottali was kept in a clean wide mouthed vessel. Gomutra was added,
till the Vatsanabha gets completely immersed in it. This vessel is kept
under sunlight whole day.
• Next day the previous Gomutra was removed and fresh Gomutra was
added and kept under sunlight. This process was repeated for three days.
• Then Vatsanabha pieces were washed with hot water and the outer layer
was removed.
• The pieces of Vatsanabha were dried under sunlight.
Observations:
• After soaking in Gomutra Vatsanabha became soft and colour of Gomutra
changed to dark brown.
• After drying Vatsanabha became black in colour.
• The Vatsanabha pieces become mrudu and tingling sensation was not felt after
Shodhana. After peeling of outer layer it had pandura varna inside.
Precautions:
• Daily Gomutra should be replaced and fresh Gomutra must be used.
• A clean wide mouthed vessel was used to avoid over flow of Gomutra..
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 65
Methodology
Test for Vatsanabha Shodhana :
It becomes so soft as it can be pierced by a pin. It does not produce tingling or
numbness when kept over the tongue.
Results :
Table No. 22, Showing Qty of Vatsanabha Before and After Shodhana
Dravya Before Shodhana
(in gms)
After Shodhana
(in gms) Loss Observations
Vatsanabha 250 180 70 Colour-Gray
Smell -Gomutra gandha.
Touch-Fine
Taste-Tikta
PRACTICAL NO. 7 VATSANABHA CHURNIKARANA
Date of commencement : 12 – 04 – 06
Date of completion : 13 – 04 – 06
Materials :
Method of preparation:
Dravya Mana
Vatsanabha 250 gms
The Grahya 250 gms of Vatsanabha churnikarana was done in Ulukalayantra
and sieved through cloth and stored in clean airtight bottle.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 66
Methodology
Observations:
Vatsanabha converts into Gray coloured fine powder.
Precautions:
Vatsanabha should be dried properly before churnikarana.
Result:
Table No. 23, Showing Quantity of Vatsanabha Before and After Churnikarana
Dravya Before Churnikarana
(in gms)
After Churnikarana
(in gms) Loss
Vatsanabha 180 160 20
PRACTICAL NO. 8 PIPPALI CHURNIKARANA
Date of commencement : 27 – 04 – 06
Date of completion : 28 – 04 – 06
Materials :
Method of preparation:
Dravya Mana
Pippali 250 gms
The Grahya 250 gms of pippali churnikarana was done in Ulukalayantra and
sieved through cloth and stored in clean airtight bottle.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 67
Methodology
Observations:
Pippali converts into brown coloured fine powder.
Result:
Table No. 24, Showing Quantity of Pippali Before and After Churnikarana
Dravya Before
Churnikarana
After
Churnikarana Loss Observations
Pippali 250 gms 190 gms 60
gms Colour-Dark green
Smell -Aromatic.
Touch-Fine
Taste-Pungent
Practical No. 9
Title : Preparation of Jatikosha churna
Date of commencement : 08 – 05 – 06
Date of completion : 09 – 05 – 06
Materials required : Ulukhala yantra,Cloth etc.
Drugs used : Jatikosha-250gms
Method:
Jatikosha was taken in ulukhala Yantra and pounding it into churna and sieved
through cloth, the procedure was repeated until complete fine powder was obtained
and stored in clean air tight container:
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 68
Methodology
Observation:
Jathi Kosha converts into brown coloured fine powder:
Precautions;
Jathikosha churna should be dried completely.
Jathikosha churna should be preserved in airtight container.
Table No. 25, . Showing the quantity of Jathikosha before and after churnikarana.
Draya Before After Loss Observation
Jathikosha 250 Gms 160Gms 90Gms Colour –Browns
Smell – Teekshna
gandi
Touch – Fine
Taste – kashaya
Step NO. 03 Preparation of Swacchanddha Bhairava Rasa (Dwitiya).
Practical No.10
Title : Swacchanddha Bhairava Rasa (Dwitiya).
Date of commencement : 15 – 05 – 06
Date of completion : 20 – 05 – 06
Reference : BhaishajyaRatnavali, 5th chapter sloka 492 – 493.
Materials required : Khalwa yantra.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 69
Methodology
Table No. 26, showing the Ingredients of Swacchanddha Bhairava Rasa (Dwitiya)
with their proportions and quantity.
Sl. Ingredients Quantity
01 Shodita Parada 50 gms
02 Shodita Gandhak 50 gms
03 Shodita Vatsnabha 50 gms
04 Pippali 50 gms
05 Jathikosha 50 gms
Method:
Kajjali was prepared.
The Homogenous mixture of Kajjali, Shodhita Vatsanabhachurna, Pippali churna,
Jatikhosha churna was prepared .
Required quantity of water is added and mardana was done until vati consistency
was achived.
The vati of 62.5 mg or ½ Ratti was prepared
The vati were kept for drying in shade.
Observations:
The whole mixture turned to black colour.
At final stage the mixture becomes a single bolus and non sticky.
At this stage small amount of mixture was taken in between hands and rolled in to
vatis.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 70
Methodology
Precautions:
Purified ingredients were used.
Care was taken that mixture is not spilled out of khalva.
Mardna was done well.
Vati was prepared immediately after mardhana.
Uniformity of weight was maintained while preparing vati. and gross verified by
the test for uniformity.
Results: 240gms of Swacchandda Bhairava rasa (Dwitiya) was obtained.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 71
Methodology
Analytical Study
Definition: 193
“Analytical chemistry may be defined as the science and art of determining the
composition of materials in terms of the elements or compounds control”.
“Analytical chemistry is a tool to gain information about the qualitative and
quantitative composition of substances and chemical species, i.e. to find out what a
substance is composed of and exactly how much”.
Analytical chemistry mainly deals with the qualitative and quantitative
assessment of chemical substance:
Qualitative Analysis: Information regarding the presence or absence of one or more
components of the sample.
Quantitative Analysis: Information, which is finally obtained by measuring same
physical property that is characteristically related to the component.
Another type of classification are194:
• Chemical methods of analysis, mainly involves gravimetric (Mass) and volumetric
(Volume) method.
• Instrumental methods, includes rest of the methods.
Ex: Diffraction of radiation: Refraction of radiation, emission and absorption of
radiation.
• The sampling, dissolution, change in oxidation state removal of excess reagent,
pH adjustment addition of complex reagents, precipitation, concentration and the
removal of interference’s are the various chemical steps which are part of an
instrumental method.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 72
Methodology
So chemical steps are often on integral part of an instrumental method, but
both the methods have their own limitations.
Steps involved in analysis:
Following are the various important steps required to reach satisfactory results:
• Obtaining the sample in pure form.
• Preparation of the sample
• Analytical procedure
• Evaluation of the satisfactory results
Importance of analytical chemistry195:
• Analytical chemistry has its impact on pharmaceutical research, quality control
and in clinical analysis.
• Sensitive chemical and instrumental tests were employed to detect abnormal and
normal components of body fluids, chemical changes occurring in the metabolic
fluids.
• In pharmaceutical industry helps in the detection of quality of the manufactured
drug in tablet, solution and emulsion form.
• In pharmaceutical studies, it is important to establish the properties and
therapeutic value of a drug before the drug is available to public.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 73
Methodology
Advantages of 196
Chemical Methods
• Procedure is accurate and simple.
• The equipment needed is cheap.
• Specialized training is usually not
required.
Instrumental Methods
• High sensitivity is obtained.
• The determination is very fast.
• Even complex samples can be handled
easily.
Limitations of
Chemical Methods
• Accuracy decreases with decreasing
amounts.
• Procedure is time consuming
• There is lack of specificity
Instrumental Methods
• The cost of equipment is large.
• Specialized training is needed.
• The sensitivity and accuracy depends
on the instrument.
Ayurveda is having its unique analytical approach towards drugs, but in
present era there is necessity of analysis of drug based on modern methodology. So,
it is a need to evaluate drugs by various parameters which includes –
1. Physical analysis
2. Chemical analysis
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 74
Methodology
Physical evaluation (Organoleptic characteristics) –
1. Colour - Black
2. Smell - Characteristic – cow’s urine
3. Touch - Smooth
01.Hardness test –
The finished vati has to be hard, as it may not disintegrate in the required
period of time and if the vati is too soft, it may not withstand during packing and
transporting Therefore, it is necessary to check hardness of tablets.
Method – (Monsanto Hardness test)
It is a soft portable hardness tester, which was manufactured and introduced
by Monsanto chemical company. It consists of a spring, which can be compressed by
moving the screw knob forward.
The tablet which to be tested is held between a fixed and a moving jaw and
reading of the indicator is adjusted to zero. The force is applied to the edge of the vati
and gradually increased by moving the screw knob forward until the vati breaks.
Reading is noted from the scale, which indicates the pressure required in Kg or in
pounds to break the tablet.
Hardness = 1.45 Kg/cm2
01. Uniform weight of tablets –
The average weight is determined by weighing 20 tablets. The tablets are also
weighed singly. The deviation from the average weight in each case is calculated and
expressed as a percentage. Not more than two of the tablets deviate form the average
weight by a greater percentage. No tablet deviate more than that percentage.
If 20 tablets are not available 10 may be used for the determination, not more
than one deviate from the average weight by a greater percentage.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 75
Methodology
Weight variation = + 6.3% to 5.7%
03. Friability –
Friability test is performed to evaluate the ability of the tablet to withstand
abrasion in packing, handling and transporting. The instrument used for the same is
known as “Friability test apparatus”
Method –
It consists of a plastic chamber, which is divided into two parts and revolves at
a speed of 25 rpm. A number of tablets are weighed and placed in the tumbling
chamber, which is rotated for four minutes of 100 revolutions. During each
revolution the tablets fall from a distance of 6 inches to undergo shock. After 100
revolutions the tablets are again weighed and loss in weight indicates the friability.
The acceptable limit of weight loss should be not more than 0.8%
Friability – Nil
04. Determination of disintegration time:
Procedure:
One pill was introduced into each tube of the disintegration apparatus. Disc
was added to each tube. The assembly was suspended in a beaker containing 0.1 N
Hcl at 370C and the apparatus was operated. The time was noted down with the help
of stopwatch. The time taken for all the tablets to disintegrate completely is
disintegration time.
Disintegration time : 25 Minutes
05. Determination of pH –
The pH value of the sample was determined by a digital pH meter. One
percent solution was prepared, as the sample was dry and solid in the form of pills.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 76
Methodology
The pills were powdered. One gram of the sample was weighed accurately and
dissolved in 100ml of water pH was noted in the digital pH meter.
PH value: 5.89 (acidic)
06. Loss on drying –
Digest pure quartz sand that passed through No.40 but 60 sieves with
hydrochloric acid wash acid free, dry and ignite preserve in a stopped bottle.
Place 25-30gms prepared sand and short glass rod in a nickel or stainless steel
dish about 55 ml diameter and 40mm deep fitted with cover. Dry thoroughly cover
dish cool in desiccators.
Pipette out of quantity of drug to yield about 1gm of dry matter mix with a few
ml of water and transfer quantitatively to the dish containing prepared sand with aid
of water. Mix the sample thoroughly with the sand.
Dry at a temperature not more than 1100C under pressure not more than 50
mm of Hg. Making trail washing at 2 hours interval. Towards end of drying period
until successive weighing do not differ by more than 2 mg. Calculate the total solid
from the loss of weight on drying.
Loss on drying: 17.08%
07. Determination of total ash.
Procedure –
Take about 2gms accurate weighed, ground drug in a previously traced silica
dish, previously ignited and weighed. Scatter the ground dry in a fine even layer on
the bottom of the dish. Incinerate by gradually increasing the heat not exceeding dull
red heat (4500C) until free from carbon. Cool and weigh Calculate the percentage of
ash with reference to the air-dried drug.
Total ash: 6.55%
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 77
Methodology
8. Identification of alkaloids
Silica get 60 F254 Merck pre-coated plates.
Mobile phase – Chloroform: Methanol (90.10)
Location reagent – Dragendroff’s reagent.
Procedure –
Weigh about 2gms of the sample into a separating flash. Add about 20ml water
Alkalize with dilute ammonia and extract with two quantities 25ml of chloroform
Filter through anhydrous sodium sulphate. Evaporate the chloroform layer to the
residue obtained. Add about 1 ml of methanol. Shake well to dissolve and spot
about 10-ul solutions on the TLC plate. Elute the plate with mobile phase to3/4” of
the plate. Dry the plate at 1050C and spray the plate with dragendroff’s regent. If
alkaloids are present brownish red sports were obtained in the sample solution.
Result : Alkaloids present.
10. Determination of Sulphur:
Eschka Mixture:
Mix two parts by weight calcined magnesia with one part of anhydrous
sodium carbonate.
Procedure:
Cover the bottom of a 50ml crucible with 0.5gm of Eschka’s mixture Weight
accurately the appropriate quantity of the sample material and mix it immediately
with 2gms of Eschka’s mixture and put evenly on the previously weighed Eschka’s
mixture Level the contents by tapping gently on a bench. Cover this uniformly with
0.5gm of Eschka mixture. Place crucible in the muffle furnace. Raise the
temperature from room temperature to 8000C +250C in about one hour and then heat
for further 90 minutes.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 78
Methodology
Transfer the ignited mixture as completely as possible from the crucible to a
beaker containing 25 to30 ml of water. Wash out the crucible thoroughly with about
50 ml of hot distilled water and add the washings to the contents of the breaker.
Add carefully sufficient quantity of concentrated hydrochloric acid to dissolve
the solid matter, warming the content of the breaker to effect solution. Boil for 5
Minutes to expel carbon dioxide. Add drop wise from a pipette, warm 5% Barium
chlorine solution. Stir the solution constantly during the addition. Allow the
precipitate to settle for a minute or two.
Then test the supernatant liquid for complete precipitation by adding a few
drops of Barium chloride solution. If a precipitate is formed, add slowly a further 3
ml of the reagent allow the precipitate to settle as before and test again, repeat this
operation until an excess of Barium Chloride is present. When an excess of the
precipitating agent has been added, keep the covered solution hot, but not boiling for
an hour (steam bath) in order to allow time for complete precipitation. The
precipitation should settle and a clear supernatant liquid should be obtained. Test the
latter with a few drops of barium chloride solution for complete precipitation. If no
precipitate obtained, the Barium sulphate is ready for filtration.
Filter the solution through an ash less filter paper (Whatman No. 42) Wash the
precipitate with small portion of hot water. Dry the paper and place it in a silica or
porcelain crucible, previously ignited to redness and cooled in a desiccators and
weighed. Gradually increase the heat until the paper chars and volatile matter is
expelled. Do not allow the paper to burst into flame as mechanical loss may thus
ensure. When charring is complete, raise the temperature of the crucible to dull
redness and burn off carbon with free excess of air. When the precipitate is white
ignite the crucible at red heat for 10-15 minutes. Allow the crucible to cool in air,
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 79
Methodology
transfer it to a desiccators and when cold, weigh the crucible and contents. Repeat
until constant weight is attained.
A blank is necessary. Calculate the percentage of sulphur converting Barium
sulphate X 0.1374.
Sulphur: 9.9%
11. Determination of mercury
Procedure –
Dissolve about 0.3gms of the sample in 5ml of aquaregia and add 100ml of
water. Add 40ml of 0.05N EDTA, 5ML OF Ammonia buffer solution and 0.5ml of
solochrome black indicator. Titrate the solution with 0.05 M Zinc sulphate until the
blue colour changes to purple (do not overshoot the end point), add 3 gms of
potassium iodide, swirl to dissolve. Allow to stand for two minutes. Then, continue
the titration with zinc sulphate solution to the same end point as before. Each ml Zinc
sulphate solution required after addition of potassium iodide = 0.0103 Hg.
Mercury : 80.3%
12. Solubility.
About one gram of the sample was weighed and dissolved in 10ml of the
solvents. When the sample did not dissolve, an excess of solvent by 10ml quantity
up to 100ml was added and noted the sample was sparingly soluble in water and
slightly soluble in chloroform and alcohol (1 gram of sample in 600 ml to 1000 ml of
chloroform and alcohol.
SOLUBILITY TESTS: RESULTS:
Water Very slightly soluble
Chloroform Sparingly soluble
Alcohol Sparingly soluble
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 80
Methodology
XRD REPORT:
x-ray diffraction method204
Definition : X-ray diffraction is a technique through which the special arrangement of
structural units of a substance in the crystalline state i.e., investigating the interior or a
crystal.
Principle :
Bragg’s law of diffraction of x-ray by crystals is applicable according to him
when an x-ray beam strikes a crystal surface at an angle portion of the beam
penetrates to the second layers of atoms and so on. The cumulative effect of this
scattering from the regularly spaced centers of the crystal is nothing but diffraction of
the beam.
The important requirement of diffraction are:
a) The Spacing between layers of atoms must be roughly the same as the wavelength
of the radiation.
b) The scattering centers must be specially distributed in a highly regular way.
Various methods of x-ray diffraction :
• Lane photographic method.
• Bragg x-ray spectrometer method.
• Ratting crystal method
• Powder method.
Sample preparation :
The samples are ground to a fine, homogenous powder and held in the beam
of thin walled glass or the specimen maybe mixed with a suitable non-crystalline
binder and moulded into a suitable shape.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 81
Methodology
As a result large number of small crystallites are oriented in all possible
directions and when x-ray beam traverses the material a significant number of
particles are expected to be oriented in such a manner that Bragg’s a equation for
reflection from every possible inter planar spacing becomes satisfied.
When the x-ray beam is diffracted by a fine powder, made of small
crystallites, diffraction will take place for all crystallites whose planes spacing of
atom d make an angle or reflection (θ) to that incident beam, and the diffracted beam
will lie on a cone of semi apex angle 2θ. The minimum interplanar spacing giving a
diffraction is at :
d = λ / 2, θ = 900
A complete study of the sample assumes all possible angular positions in the
path of the x-rays, should give a unique result for each substance.
Applications :
• X-ray diffraction provides a convenient and, practical means for qualitative
identification of crystalline compounds where the x-ray diffraction pattern is
unique for each crystalline substance.
• Quantitative analysis of x-ray diffraction is done by comparing the intensity of a
chosen diffraction line in a standard mixture.
• X-ray diffraction is employed in investigating the interior of a crystal. (size and
shapes of individual crystal vary but interfacial angle remain constant).
• Used in detecting the structures of complex natural products such as steriods,
vitamins and antibiotics.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 82
Methodology
Advantages:
• X-ray methods are non-destructive.
• X-ray analysis done to crystalline samples in any physical state of sub-division.
Disadvantages:
• The accuracy of the analysis depends on the surface preparation, reliability of
standards, stability of x-ray tube output and the number of x-ray photos counted.
• Instrumental and sample variable affect the analysis.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 83
Discussion
Discussion
In the present work swacchandha Bhairava Rasa (Dwitiya) 1 is taken for
pharmaceutical and analytical study, for its Jwaraghna property as it is indicated in
navajwara. The ingredients of Swacchandda Bhairava rasa (Dwitiya) are Shodhita
hingulota parada, Shodhita gandhaka, Shodhita vatsanabha, pippali and jatikosha.
Jwara is a common entity found in day-to-day practice, as a disease and also as a lakshna
in other diseases, where agnimandhya is a root cause, which forms ama, by virtue of it
srotoavarodha and swedavarodha, leading to the raise in body temperature.
As Swacchandda Bhairava rasa (Dwitiya) acts as jwaraghna by their properties i.e.
Deepana pachana properties causes Agnivardhana and their by Amapachana.
Swedapravartaka properties relieving sweedavarodha there by reducing body
temperature.
Due to rasayana property, its rasayana karma helps in normalizing Dhatvagnipaka.
Yogavahi guna enhances the properties of other ingredients there by cumulating the
jwaraghna effect.
Hence it is a need of hour to critically analyze the Swacchandda Bhairava rasa (Dwitiya)
by attempting through proper pharmaceutical and necessary analytical approach.
We shall discuss each of these ingredients individually & their shodana, specialized
combinations, final combination, pharmaceutical studies & analytical reports in a
stepwise fashion.
Hingula:
• In samhita kala there were no reference of Hingula, but we get the reference of
parada.
• It is chief ore of Mercury and combination of mercury and Sulphur.
• In olden days it was assumed to be imported from other countries.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 84
Discussion
Hingula shodhana:
Shodhana was done with nimbuswarasa, due to bhavana, hingula was reduced to
finer particles, which helps to extract maximum amount of mercury. The increase of
weight after shodhana is by addition of solid contents of nimbuswarasa.
Hingulottha parada:
• As the name indicates the parada is extracted from Hingula
• Hingula taken was of kritrima variety.
• Method used for Hingulakarshana was urdhwa paatana vidhi as per Rasatarangini
• Urdhwa patana yantra was used for the Hingulaakarshana
• Yield of parada by this process was 52%
• Loss is due to insufficient heating pattern, unburnt chakrikas and some portion by
dhoomagati, malagati and jalagati in process.
Parada Shodhana
• Parada Shodhana was done as per rasatarangini, with haridrachoorna and
nimbuswarasa.
• Properties of haridra embides the jwaragna property in parada by shodhana.
• Loss of parada is due to malagati and jalagati in the shodhana process.
• The parada was looking brighter & more glistening after the shodhana.
Gandhaka Shodhana
• Gandhaka taken for the study was checked for the lakshanas of grahya gandhaka
• Amlasara gandhaka was considered for the study & subjected for shodhana as per
rasatangini
• Gandhakashodhana was done with equal quantity of godugda & ghritha
• Shuddha gandhaka was brighter, spotless & softer than ashuddha gandhaka
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 85
Discussion
• Shuddha Gandhaka had lesser gandha than Ashuddha gandhaka
• Shuddha gandhaka was pindakriti.
• Teekshna ushna gunas of gandhaka reduces by demulcent properties of dugdh and
gritha
• Fat soluble impurities get removed by shodhana
• Loss is due to removal of impurities, some portions due to washing and some due to
adhesion to the vessel and cloth.
KAJJALI:
• Kajjali was observed for siddhi laskshanas like nishchandratva, rekhapurnatva,no
free mercury particles seen after rubbing it with water on hand.
• Loss is due to spilling out from khalva during process .
• Regarding Khalvee Rasayana
Kajjali should be very fine like collyrium, absence of dazzling particles. In different
medicines, purified mercury and purified sulphur are mixed in different proportions.
Addition of Kajjali along with herbal ingredients helps to increase their durability adds
shelf life and reduces their dosage. Kajjali as such is used in combination with other
drugs in a recipe.
Vatsanabha Shodhana:
• Vatsanabha is known to Ayurvedic pharmacopeia since long ago. Charaka
classifies it under sthavara visha.
• Sushruta classified it under kanda visha; Sthavara vishas are used mainly in
therapeutics.
• Among sthavara visha kanda and moola vishas are very commonly as they are
claimed to be as more potent and effective.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 86
Discussion
• Amrita synonym of vatsanabha, quoted by Rasatarangini and Dhanwantari
Nighantu, if it is administered after purification with proper dose and precautions, it
acts quick in the body, proves highly beneficial to body.
• Some acharyas termed marana, pranahara, for vatsanabha. Because it is visha
dravya.
• In samihita kala the use of Vatsanabha for therapeutic purpose was limited because
of less development of shodhana procedures.
• Shodhana is necessary for Vatsanabha before using therapeutically. Ashodhita
visha when used may lead to several complications. Purification’s is done to reduce
its toxicity.
• While explaining grahya vatsanabha it is sthoola, snigdha, guru, naveena.
• Timing of collection: After fruiting
• Condition: free from kita etc,
• Best variety: pandu variety.
It may be that it is less toxic to other varieties and gives more therapeutic effects.
Vatsanabha indicated in
• Vatarakta, Shwasa, kasa, does the prashamana of Jwara and Amavata.
• It may be due to the property of diaphoretic, antipyretic, anti inflammatory in small
doses. It is narcotic when administered in large doses.
• Loss is due to drying, peeling of outer layer and while pounding.
Pippali-
• Pippali is used as a drug in Charaka, Sushruta and Vagbhata samhitas, usually used
dravya in many Ayurvedic formulations.
• Magadhi synonym quoted by Dhanwantari nighantu and Bhavaprakash nighantu, it
shows the place of avaliability.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 87
Discussion
• Pippali has the property of Rasayana, Jwarahara, Kasashwasahara, Shoola Amavata.
• It is stimulant, carminative, and also immuno-modulator.
• Loss is due to spilling out while pounding and sieving.
JatiKosha :
JatiKosha is explained in Charaka, Sushruta and Vagbhata samhitas, usually used
dravya in many Ayurvedic formulations.
Loss is due to spilling out while pounding and sieving.
Preparation of Swacchandda Bhairava rasa (Dwitiya)
After mixing the ingredients homogeneously mardhana with jala was done till it attains
vati consistency and rolled into vatis.
240gms of Swacchandda Bhairava rasa (Dwitiya) was obtained.
Gain of weight is due to jala bhavana.
Discussion on analytical study-
Uniformity of weight
For the present study vatis were prepared manualy. 20 vatis taken randomly and weighed.
The average weight was calculated. The weight variations fall with in normal limits.
Hardness test
Swacchandda Bhairava rasa (Dwitiya) was subjected to hardness test, values were
observed as 1.45 kg./Cm2
Disintegration test :
Vati disintegrated within 25 minutes in 0.1N Hcl this is within normal limits.
Friability test
The weight of the tablets weighed before and after 100 revolutions showed weight loss of
Nil which shows that it can withstand abrasion in packing, handling and transporting.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 88
Discussion
pH test:
the pH of the vati is 5.89. It is acidic in nature
Loss on drying
It shows the end product contain 17.8 % if moisture.
Alkaloids
The formulation contain Parada,Gandaka, Vatsanabha, Pippali & Jathikosha, Alkaloids
of all the dravyas were restored even in the end product, which was confirmed by TLC
procedure.
Total ash
The herbal drugs were converted in to ash form resulting in to weight loss. Here value of
weight loss noted is 6.55%.
Solubility test:
SOLUBILITY TESTS: RESULTS:
Water Very slightly soluble
Chloroform Sparingly soluble
Alcohol Sparingly soluble
Assay for Mercury and Sulphur
The percentage of Mercury and Sulphur was assayed in the vati which revealed that
Mercury was present in 80.3% and Sulphur was present in 9.9%.
XRD:
The report suggest that the graph is specific to Swacchandda Bhairava rasa (Dwitiya).
The highest peak level is of parada, rest of the peak is of other ingredients in their
complex state present in Swacchandda Bhairava rasa (Dwitiya)
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 89
Discussion
PROBABLE MODE OF ACTION:
Swacchandda Bhairava rasa (Dwitiya)
Ingredients Guna karmas
Parada Yogavahi, Sarvamayahara, rasayana.35
Gandaka Kaphavatahara, rasayana.44
Vatsanabha Yogavahi, Pranadayi, Tridoshaghna.79
Pippili Deepana, rasayana, Vatasleshmahara,
Jwaraghna.99
Jatikosha Ruchi varna kruth,vishapaha104
Deepana and pachana action from parada, gandhaka, vathsanabha, pippali and
jatikosha causes amapachana action, this helps chikithsa sutra of navajwara. Once the
amapachana is done the niramalakshanas would appear.
Shrothoavarodha in the form of swedaavarodha in navajwara which needs ushna,
theekshana gunas to contaract. Pippali, Vathsanabha and jatikosha have ushana
theekshana gunas which has lekhana action, this reduces the swedaavarodha.
Vathsnabha with its swedapravartaka action would ease the flowing out of sweda,
which in turn carries the pitta out of the srotases. This helps to reduce santapa in jwara.
• Yogavahi Guna enhances activities of other ingredients that are combined with it in
this case parada, Vatsanabha enhances activities of Jathikosha pippli and Gandaka
without loosing their own karmas.
• In Navajwara as dosha is pitta and Dhathu being rasa, a rasayana is the need of the
hour as it corrects the Agni which has becomes dushya in Jwara.
• Jwaragna activities of pippli and vatsanabha is enhanced by Yogavahithva of Kajjali.
• Vishapaha guna of Jathi kosha rectifies the vitiated aama which is cause of
Navajwara
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 90
Discussion
There by the yoga Swacchandda Bhairava rasa (Dwitiya) can effectively combact the
disease navajwara.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 91
Conclusion
Conclusion:
“Pharmaceutico – Analytical study on Swacchandda Bhairava rasa (Dwitiya)”
1. The formation of Swacchandda Bhairava rasa (Dwitiya) has a balance of drugs
acting on the samprapthi of Navajwara to bring about samprapthi vighatana.
2. Hingula is a chief ore of parada.
3. Hingula available in the modern days is kritrima Hingula.
4. Urdhwapatana yantra is the Yantra useful in Hingulakarshana.
5. 52% is the yield in this process.
6. The loss of parada in this process happens due to dhooma, mala and jalagati of
parada.
7. Sodhana Hingulakarshitha parada should be done with Haridra churna and
nimbu swarasa.
8. Haridra turns greenish, latter brownish colour when triturated with parada and
nimbu swarasa.
9. Parada becomes brighter after shodhana by the above method.
10. The loss of parada through this process is roughly 13%, which may be due to
jala and mala gati.
11. Shodhana of Gandaka with gritha and dugdha makes it brighter, softer,
spotless, less odour, less teekshna than ashudda gandaka.
12. Loss of gandaka in the above process is 18 %
13. He takes 72 hours to reach Kajjali Siddi lakshana at the rate of 14 to 15 strokes
per minute while mardhana.
14. Loss of Kajjali while mardhana is roughly 8 % due to spillage.
15. Shodhana of vatsanabha with Gomutra abolishes the tingling effect on tongue
when applied.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 92
Conclusion
16. The loss due to shodhana of vatsanabha is around 28% w/w
17. Suddha vastsanabha is darker, softer.
18. The ingredients of Swacchandda Bhairava rasa (Dwitiya) were added in the
same chronological order as in the classical.
19. After adding the ingredients of Swacchandda Bhairava rasa (Dwitiya)
mardhana was done with jala for 3 hours.
20. The bolus of Swacchandda Bhairava rasa (Dwitiya) after attaining vati
consistency, can be rolled into pills easily and bolus is almost non sticky.
21. There was about 4% loss of the material in the final product during the process
of yoga.
22. Swacchandda Bhairava rasa (Dwitiya) analysis reveals the hardness – 1.45 kg
/cm2 , disintegration time – 25 minutes in 0.1 N Hcl solution, friability
passes the test loss on drying – 17.8%, test for alkaloides – present, total ash-
6.55% , solubility test-inwater very sparingly soluble, in cloroform and
alcohol sparingly soluble, assay for mercury – 80.3% and for sulphur – 9.9%
SCOPE FOR FURTHER STUDY:
• The yoga can be further taken for toxicity study.
• Experimental model for antipyretic effect can be undertaken.
• Its efficacy can be ruled out Clinically.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 93
Summary
SUMMARY:
The present study is entitled “Pharmaceutico analytical study on Swacchandda
Bhairava rasa (Dwitiya).”In this study, here an attempt is made to prepare Swacchandda
Bhairava rasa (Dwitiya) as per classical procedure and its necessary physical and
chemical analisis was carried out.
This study includes the following chapters viz.Introduction, Objectives, Review
of literatureand methodology which contains pharmaceutical and analytical study. The
next chapter discussion and conclusion.
1.In the introduction part importance of rasa shastra, Khalvi rasayana & Kajjali kalpas is
explained.
2.Aims and objectives of present study are mentioned in objective chapter.
3.Revive of literature deals with drug revive i.e. the ingredients of Swacchandda
Bhairava rasa (Dwitiya), their vernacular name synonyms varities shodhana properties
and modern view is described.
4.Methodology it deals about pharmaceutical and analytical study.
a.in pharmaceutical study explains about hinguja shodhana, hingulotha parada, parada
shodhana, gandhaka shodhana,kajjali preparation, vatsanabha shodhana and choornikarna
,pippali choornikarna ,jathikosha choornikarna and
Preparation of Swacchandda Bhairava rasa (Dwitiya).
b. The analytical study deals about physico chemical analysis of Swacchandda Bhairava
rasa (Dwitiya), i.e.pH, total ash, friability, estimation of mercury and sulphur etc
c. Discussion chapter deals with discussions regarding ingredients of Swacchandda
Bhairava rasa (Dwitiya), process of pharmaceutical and analytical study.
Finally essence of dissertation is explained in conclusion.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 94
Bibilography
BIBILOGRAFIC REFERENCES.
1. Sri Govindadas Bhaisajya Ratnavali, 5th Chapter, sloka 492-493, Edited
by Ambikadatta Shastri, 7th Edition Varnasi, Chaukamba Sanskrit Prakashan
P.83.
2. Sri Rasavagbhata, Rasaratna Samuchaya, 3rdChapter sloka126-127, edited by
Indra Dev Tripati, 2nd Edition Varnasi Chaukamba Sanskrit Prakashan 2003.
P.37.
3. Sri Sadanandarsharma, Rasa Tarangini, 9thTaranga, sloka1-2, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas
publications, 2000. P.198.
4. Yadavji Trikrmji Acharya, Rasamrutham, 1st Chapter, edited by Sri Damodar
Joshi, Ist Edition, Varanasi, Chaukamba Sanskrit Prakashan, 1998 .P.26-27.
5. Sri Sadanandarsharma, Rasa Tarangini, 9thTaranga, sloka 4, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas
publications, 2000. P.199.
6. Sri Rasavagbhata, Rasaratna Samuchaya, 3rdChapter sloka 147-148, edited by
Indra Dev Tripati, 2nd Edition Varnasi Chaukamba Sanskrit Prakashan 2003.
P.41.
7. Acharaya Sri Madhava, Ayurveda Prakasha, 2nd Chapter, Sloka 70-71,
edited by Gulraj Sharma Mishra, 2nd Edition, Varanasi, Chaukamba Bharath
Academy, 1999 . P.272-273.
8. Sri Sadanandarsharma, Rasa Tarangini, 9thTaranga, sloka 3, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas
publications, 2000. P.199.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 95
Bibilography
9. Bhava mishra Bhava prakash Nighantu, Datwadivarga, sloka 104, edited by
G.S. Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy, 1984.P.615.
10. Sri Sadanandarsharma, Rasa Tarangini, 9thTaranga, sloka 13-17, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas
publications, 2000. P.201.
11. Sri Rasavagbhata, Rasaratna Samuchaya, 3rdChapter, sloka 152-153, edited by
Indra Dev Tripati, 2nd Edition Varnasi Chaukamba Sanskrit Prakashan 2003.
P.41.
12. Acharaya Sri Madhava, Ayurveda Prakasha, 2nd Chapter, Sloka72, edited
by Gulraj Sharma Mishra, 2nd Edition, Varanasi, Chaukamba Bharath
Academy, 1999 P.274.
13. Sri Sadanandarsharma, Rasa Tarangini, 9thTaranga, sloka 49, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas
publications, 2000. P.207.
14. P.L Sony, Sultan Chand and Son’s, Text book Inorganic Chemistry, 20th Edn,
New Delhi, 1991.P. 3.324-3.336.
15. Acharaya Sri Madhava, Ayurveda Prakasha, 2nd Chapter. Sloka 78-82, edited
by Gulraj Sharma Mishra, 2nd Edition, Varanasi, Chaukamba Bharath
Academy, 1999, P.276.
16. Gopal krishna, Rasendrasarasangraha, 1st Chapter, Sloka 51-53, edited by
Brahmashankar Mishra, 6th Edition varanasi, Chaukamba Amarabharati
Pralashan, 1981. P-14.
17. Sri Sadanandarsharma, Rasa Tarangini, 5thTaranga, sloka 38-39, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas
publications, 2000. P.82.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 96
Bibilography
18. Sri Rasavagbhata, Rasaratna Samuchaya, 3rdChapter, sloka 144, edited by
Indra Dev Tripati, 2nd Edition Varnasi Chaukamba Sanskrit Prakashan 2003.
P.69.
19. P.L Sony, Sultan Chand and Son’s, Text book Inorganic Chemistry, 20th Edn,
New Delhi, 1991.P. 3.324-3.325.
20. Koutilya arthashastra, 34th chapter.
21. Charaka’s, Charaka Samhita with Vidhyotini Hindi Commentary by Kashinath
Shastri and Goraknath Chaturvedi, 7th Chapter, sloka 79, 16th Edn, Varanasi,
Chaukamba Bharati Academy, 1998.P.
22. Acharya Susruta’s, Sushrutha Samhita,chikitsastana, 25th Chapter,sloka 39,
Edited by Ambikadatta Shastry, 11th Edn, Varanasi, Chaukamba Sanskrit
Samsthana, 1997, P.117.
23. Yadavji Trikrmji Acharya, Rasamrutham, 1st Chapter, edited by Sri Damodar
Joshi, Ist Edition, Varanasi, Chaukamba Sanskrit Prakashan, 1998 .P-3.
24. Sri Rasavagbhata, Rasaratna Samuchaya, 1st Chapter, sloka 76-78, edited by
Indra Dev Tripati, 2nd Edition Varnasi Chaukamba Sanskrit Prakashan 2003.
P.8.
25. Sri Sadanandarsharma, Rasa Tarangini, 5thTaranga, sloka 2-4, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas
publications, 2000. P.71-72.
26. Ambikadatta Shastry, Rasa Ratna Samuchchya, 1st Chapter, sloka 67, 9th Edn,
Chaukambha Amarabharati publication, Varanasi, 1995,P.190-191.
27. Sri Rasavagbhata, Rasaratna Samuchaya, 1st Chapter sloka 68-73, edited by
Indra Dev Tripati, 2nd Edition Varnasi Chaukamba Sanskrit Prakashan 2003.
P.7-8.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 97
Bibilography
28. Sri Rasavagbhata, Rasaratna Samuchaya, 11thChapter, sloka 19-22, edited by
Indra Dev Tripati, 2nd Edition Varnasi Chaukamba Sanskrit Prakashan 2003.
P.121.
29. Sri Rasavagbhata, Rasaratna Samuchaya, 11th Chapter sloka 19, edited by
Indra Dev Tripati, 2nd Edition Varnasi Chaukamba Sanskrit Prakashan 2003.
P.121.
30. Sri Rasavagbhata, Rasaratna Samuchaya, 11th Chapter, sloka 21, edited by
Indra Dev Tripati, 2nd Edition Varnasi Chaukamba Sanskrit Prakashan 2003.
P.121.
31. Sri Rasavagbhata, Rasaratna Samuchaya, 11th Chapter sloka 25, edited by
Indra Dev Tripati, 2nd Edition Varnasi Chaukamba Sanskrit Prakashan 2003.
P.121.
32. Gopal krishna, Rasendrasarasangraha, 1st Chapter, Sloka 9, edited by
Brahmashankar Mishra, 6th Edition varanasi, Chaukamba Amarabharati
Pralashan, 1981 P.3.
33. Ibid.
34. Sri Sadanandarsharma, Rasa Tarangini, 5thTaranga, sloka 40-41, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas
publications, 2000. P.82.
35. Bhava mishra Bhava prakash Nighantu, Datwadivarga, sloka 91-92, edited by
G.S. Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy, 1984.P.613.
36. Yadavji Trikrmji Acharya, Rasamrutham, 1st Chapter, Sloka 2-3, edited by
Sri Damodar Joshi, Ist Edition, Varanasi, Chaukamba Sanskrit Prakashan,
1998 .P.4.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 98
Bibilography
37. R.Ghosh,Pharmacology, Materia Medica and Therapeutics, Edited by
S.G.S.Gupta, 23rd ed, Calcutta , Hilton & co.,1976.P935-937.
38. K.M. Nadakarni, Indian Materia Medica,Volume-II, edited by A.K. Nadakarni
3rd edition Mumbai, Popular Prakashan 1996.P.72-83.
39. Sri Rasavagbhata, Rasaratna Samuchaya, 3rd Chapter sloka 3-12, edited by
Indra Dev Tripati, 2nd Edition Varnasi Chaukamba Sanskrit Prakashan 2003.
P.26-27.
40. Yadavji Trikrmji Acharya, Rasamrutham, 2nd Chapter,edited by Sri Damodar
Joshi, Ist Edition, Varanasi, Chaukamba Sanskrit Prakashan, 1998 .P.29.
41. Sri Sadanandarsharma, Rasa Tarangini, 8thTaranga, sloka 1-3, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas
publications, 2000. P.174-175.
42. Sri Rasavagbhata, Rasaratna Samuchaya, 3rd Chapte,r sloka 13-16, edited by
Indra Dev Tripati, 2nd Edition Varnasi Chaukamba Sanskrit Prakashan 2003.
P.26-27.
43. Acharya Madhava , Ayurveda Prakasha, 2ndChapter, Sloka 20, edited by Sri
Gulraj Sharma Mishra 2nd Edition, Varanasi Chaukamba Bharat Academy –
1999. P.261.
44. a. Bhava mishra Bhava prakash Nighantu, Datwadivarga, sloka 111, edited by
G.S. Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy, 1984.P.616.
b. Sri Rasavagbhata, Rasaratna Samuchaya, 3rd Chapter sloka 18, edited by
Indra Dev Tripati, 2nd Edition Varnasi Chaukamba Sanskrit Prakashan 2003.
P.27.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 99
Bibilography
45. a. Sri Bhudev Devasharmana Mukhyopodyaya, Rasa Jala Nidhi, Vol- II,
2ndChapter, sloka131-132, Edited by Siddhinandan mishra, 2nd ed, Varansi, Sri
Gokul Mudranalaya, 1984. P.
b. Acharya Madhava , Ayurveda Prakasha, 2ndChapter, Sloka 18, edited
by Sri Gulraj Sharma Mishra 2nd Edition, Varanasi Chaukamba Bharat
Academy –1999. P.260.
c. Sri Sadanandarsharma, Rasa Tarangini, 8thTaranga, sloka 5-6, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas
publications, 2000. P.175.
46.a. Sri Sadanandarsharma, Rasa Tarangini, 8thTaranga, sloka 7-11, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas
publications, 2000. P.176.
b. Sri Rasavagbhata, Rasaratna Samuchaya, 3rd Chapter sloka 21-23, edited by
Indra Dev Tripati, 2nd Edition Varnasi Chaukamba Sanskrit Prakashan 2003.
P.28.
c. Sri Sadanandarsharma, Rasa Tarangini, 8thTaranga, sloka 21-22, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas
publications, 2000. P.178.
47. Acharya Madhava , Ayurveda Prakasha, 2ndChapter, Sloka 25, edited by
Sri Gulraj Sharma Mishra 2nd Edition, Varanasi Chaukamba Bharat Academy
–1999. P.262.
48. Sri Sadanandarsharma, Rasa Tarangini, 8thTaranga, sloka 39, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas
publications, 2000. P.182.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 100
Bibilography
49. Acharya Madhava, Ayurveda Prakasha, 2ndChapter, Sloka 49-50, edited by
Sri Gulraj Sharma Mishra, 2nd Edition, Varanasi Chaukamba Bharat
Academy –1999. P.268.
50. P.L.Sony,Text book of Inorganic Chemistry,Sultan chand and son’s,20th
ed, New Delhi,1991.P.2.561.
51. K.M. Nadakarni, Indian Materia Medica,Volume-II, edited by A.K.
Nadakarni 3rd edition Mumbai, Popular Prakashan 1996.P.120.
52.http:|| www.du.edu./ -jcalvert / phys / Sulphur.htm.
53. Bhava mishra Bhava prakash Nighantu, Amlavarga, edited by G.S.
Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy, 1984.P595-596.
54. Pandit Narahari, Raja Nighantu, Amradivarga, Sloka 173, edited by
Indradev Tripati , 2nd ed, Varanasi , Krishnadasa Academy, 1998. P.375.
55. J.L.N Shastry, Dravaguna Viganana, Vol-II, edited by K.C. chunekar Ist
ed, Varanasi Chaukamba Orientalia, 2004. P.106.
56.a.ibid
b. Bhava mishra Bhava prakash Nighantu, Amradiphala Varga, sloka136,
edited by G.S. Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy,
1984.P.595.
57. Bhava mishra Bhava prakash Nighantu, Haritakyadi Varga, sloka 196-198,
edited by G.S. Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy,
1984.P.114.
58.a. Pandit Narahari, Raja Nighantu, Pippalyadi Varga, Sloka 196-198, edited
by Indradev Tripati , 2nd ed, Varanasi , Krishnadasa Academy, 1998. P.174.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 101
Bibilography
b. Bhava mishra Bhava prakash Nighantu, Haritakyadi Varga sloka 196,
edited by G.S. Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy,
1984.P.114.
59. J.L.N Shastry, Dravaguna Viganana, Vol-II, edited by K.C. chunekar Ist
edition, Varanasi; Chaukamba Orientalia, 2004. P.514.
60.a. Pandit Narahari, Raja Nighantu, Pippalyadi Varga, Sloka 199, edited
by Indradev Tripati , 2nd ed, Varanasi , Krishnadasa Academy, 1998. P.175.
b. Bhava mishra Bhava prakash Nighantu, Haritakyadi Varga sloka 196,
edited by G.S. Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy,
1984.P.114.
61. Bhava mishra, Bhava prakash Nighantu, Dugda Varga sloka 196, edited
by G.S. Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy, 1984.P.759.
62.a. Bhava mishra, Bhava prakash Nighantu, GritaVarga, sloka 4-6,edited by
G.S. Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy, 1984.P.775.
b.Anand,seminar book, Role of pure Ghee, Health and Nutrition, 13-14
June,2002.p.2.
63. K.M. Nadakarni, Indian Materia Medica, Volume-II, edited by A.K.
Nadakarni, 3rd edition, Mumbai, Popular Prakashan 1996.P.500.
64. Sri Rasavagbhata, Rasaratna Samuchaya, 8thChapter, sloka 5, edited by
Indra Dev Tripati, 2nd Edition Varnasi Chaukamba Sanskrit Prakashan 2003.
P.145.
65. Sri Sadanandarsharma, Rasa Tarangini, 6thTaranga, sloka 110-111, edited
by Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas
publications, 2000. P.125.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 102
Bibilography
66.a. Sri Sadanandarsharma, Rasa Tarangini, 6thTaranga, sloka 112, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas
publications, 2000. P.176.
b. Yadavji Trikrmji Acharya, Rasamrutham, 1stChapter, sloka 18,edited by Sri
Damodar Joshi, Ist Edition, Varanasi, Chaukamba Sanskrit Prakashan, 1998.
P.14.
67. Sri Sadanandarsharma, Rasa Tarangini, 6thTaranga, sloka 112, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas
publications, 2000. P.126.
68.Agnivesha, Charaka samhita, chikitsasthana, 23rd chapter, shloka 11-12,
editor Kashinatha Shastri and Gorakhanatha Chaturvedi, 12th edition,
Varanasi: Choukhamba Bharatee Academy, 1996, p. 625.
69. Sushruta, Sushruta samhita, Kalpasthana, chapter 2, shloka 5, editor
Ambikadatta Shastri, 11th edition, Varanasi: Choukhamba Saskrit Samsthana,
1997, p. 17.
70 a. P.V.Sharma, Dhanwantari Nighantu, Mishrakadi Varga, Sloka 111,
edited by Guruprasad Sharma, 3rd ed. Varnasi Chaukamba Orientalia, 2002.
P.280.
b. Pandit Narahari, Raja Nighantu, PippalyadiVarga, Sloka 222, edited by
Indradev Tripati , 2nd ed, Varanasi , Krishnadasa Academy, 1998. P.179.
c. Sri Sadanandarsharma, Rasa Tarangini, 24th Taranga, sloka16, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas
publications, 2000. P.651.
d. Bhava mishra Bhava prakash Nighantu, Datwadi Varga sloka 1, edited
by G.S. Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy, 1984.P629.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 103
Bibilography
71. Bhava mishra Bhava prakash Nighantu,Dhatwadi Varga ,edited by G.S.
Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy, 1984.P.629.
72. Yogaratnakara, Yogaratnakara, Poorvardha, shloka 2, edited by Laxmipati
Shastri, 5th edition, Varanasi, Choukhamba Sanskrit Samsthana, 1993, p. 165.
73.Sri Sadanandarsharma, Rasa Tarangini, 24thTaranga, sloka15, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas publications,
2000. P.650.
74. Acharya Madhava , Ayurveda Prakasha, 6thChapter, Sloka 17, edited by Sri
Gulraj Sharma Mishra 2nd Edition, Varanasi Chaukamba Bharat Academy –1999.
P.48.
75.a. Sri Sadanandarsharma, Rasa Tarangini, 24thTaranga, sloka10-11, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas publications,
2000. P.649.
b. Bhava mishra Bhava prakash Nighantu, Datwadi Varga sloka 19,
edited by G.S. Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy,
1984.P.630.
76. JLN Shastri, Dravyaguna Vignana, 1st edition, Varanasi : Choukhamba
Orientalia, 2004, p. 452.
77. Sri Sadanandarsharma, Rasa Tarangini, 24thTaranga, sloka18, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas publications,
2000. P.651.
78a.Sri Sadanandarsharma, Rasa Tarangini, 24thTaranga, sloka19-25, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas publications,
2000. P.625.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 104
Bibilography
b.Vaidya Yadavaji Trikamji Acharya, Rasamritam. Edited by Damodar Joshi, 1st
edn. Varanasi: Choukamba Sanskrit Sansthana ; 1998. Rasayo vignaneeyam. p.
282-283.
c.Prof. P.V. Sharma, Dravyaguna vignana, Vol- II. 1st edn. Varanasi: Choukambha
Bharati Academy ; 1981. p.110
d.Yogaratnakara. Edited by Shri Laxmipati Shastry. 5th edn. Varanasi:
Choukamba Sanskrit Sansthana; 1993. p. 241.
e.Bhudeb Mookarje, Rasajalanidhi Vol-II, 2nd edn. Varanasi : Shri Gokul
Mudranalaya ; 1984.Vatshanabha. p.339.
79. P.V.Sharma, Dravyaguna Vignana Vol-II, 1st edition, Varanasi : Choukhamba
Bharateeya Academy, 1981,2nd chapter, p. 107.
80.P.V. Sharma, Dhanwantari Nighantu, Mishrakadi varga, shloka 11-12, editor
Guruprasada Sharma, 1st edition, Varanasi: Choukhamba Orientalia, 1982, p. 280.
81.Pandita Narahari, Rajanighantu, Pippalyadivarga, shloka 223, edited by
Indradeva Tripathi, 2nd edition, Varanasi: Krishnadasa Academy, 1998, p. 180.
82.Sadananda Sharma, Rasatarangini, 24th Taranga, shloka 26-31, edited by
Kashinatha Shastri, 11th edition, Varanasi: Motilala Banarasi Das, 1979, p.653.
83.K.M. Nadakarani, Indian Materia Medica, Vol - I, editor A. K. Nadakarni, 3rd
edition, Bombay: Popular Prakashana Private Limited, 1982, p. 23.
84. Sri Sadanandarsharma, Rasa Tarangini, 24thTaranga, sloka 64-66, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas publications,
2000. P.659.
85. Sri Sadanandarsharma, Rasa Tarangini, 24thTaranga, sloka 61-63, edited by
Kashinathan shastri, 11th Edition, New Delhi, Motilal Banarasidas publications,
2000. P.659.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 105
Bibilography
86. Sushruta, Sushruta samhita, Kalpasthana, 2nd chapter , shloka 12,editor
Ambikadatta Shastri, 11th edotion, Varanasi : Choukhamba Saskrit Samsthana,
1997 p. 20.
87. Bapalala Bahisha, Nighantu Adarsha, Poorvardha, 1st edition, Varanasi:
Choukhamba Vidyabhavana, 1981, Vatsanabha varga, p. 4.
88. Vagbhatacharya, Rasaratna Samucchaya, editor Ambikadatta Shastri, 9th
edition, Varanasi: Choukhamba Ambarabharati Prakashana, 1998, chapter 29th,
shloka 145, p. 602.
89 Krishnan Vij., Textbook of Forensic Medicine and Toxicology, 2nd edition,
Delhi B. Chirchill’s Livingstone Private Limited, 2002, 12th chapter, p. 940-942.
90. Pandita Narahari, Rajanighantu, , Ksheradi varga, shloka 8, edited by
Indradeva Tripathi, 2nd edition, Varanasi : Krishnadasa Academy, 1998, p. 505.
91. Bhava mishra Bhava prakash Nighantu, Mutra Varga, sloka 1, edited by G.S.
Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy, 1984.P.778.
92. Agnivesha, Charaka samhita, Ayurveda deepika commentary, Sutrasthana 1st
chapter, sloka 101,editor P.V.Sharma, 5th edition, Varanasi : Choukhamba
Sanskrit Samsthana, 1997, p. 35.
93. K.M. Nadakarani, Indian Materia Medica, Vol - I, editor A. K. Nadakarni, 3rd
edition, Bombay: Popular Prakashana Private Limited, 1982, p. 232-233.
94.a. Agnivesha, Charaka samhita, editor Gangasahya Pandyeya, Sutrasthana,
chapter 4th chapter, shloka 6,9,25,29,30,36,42,45, 2nd edition, Varanasi:
Choukhamba Sanskrit Samsthana, 1983, p. 60-68
b. Sushruta, Sushruta samhita, editor Ambikadatta Shastri, , Sutrasthana,
chapter 39, shloka 22&60, 11th edition, Varanasi: Choukhamba Saskrit
Samsthana, 1997 p. 143&145.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 106
Bibilography
c. Vagbhatacharya, Ashtangasangraha, Sutrasthana, chapter 16th, shloka 40,
editor Ravidatta Tripathi, 2nd edition, Varanasi: Choukhamba Sanskrit Series,
1992, p. 324.
95. Bhava mishra Bhava prakash Nighantu,Haritakyadi Varga ,edited by G.S.
Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy, 1984.P.15
96. a. Bhavamishra, Bhavaprakasha Nighantu. Haritakyadi varga. Sholka 55-58. Edited by G.S. Pande., 7th edn Varanasi : Choukambha Bharati Academy ; 1982 p. 15.
b. P. V. Sharma, Kaidev Nighantu, Aoushadhi varga, Shloka 1165-1169, 2nd edn. Varanasi : Choukambha Orientalia ;. p.215.
c. Bapalal G. Vaidhya, Nighantu Adarsha pippalyadi varga, 1st edition. Varanasi : Choukambha Bharati Academy ; 1985,. p.353
d. Pandit Narahari’s Rajanighantu, Pippalyadi varga, Shloka-11-12, edited by Indradeva Tripathi, 2nd edn. Varanasi: Krishnadasa Academy; 1998. p.135-136.
e. Dhanavantari Nigantu, P. V. Sharma Shatapushpadi Varga, Shloka 73,1st edn. Varanasi: Choukambha Bharati Academy; 1982, p. 83.
f. Madanapala, Madanapala nighantu, Shloka 12, edited by Pandith Ramaprasad Vaidhya Upadhyaya, Mumbai: Khemaraja Sri Krishnadas Prakashan;. P.66.
97. Pandita Narahari, Rajanighantu, Pippalyadi varga, shloka 1, edited by
Indradeva Tripathi, 2nd edition, Varanasi: Krishnadasa Academy, 1998, , p. 135-
136.
98.a. P.V.Sharma, Dravyaguna Vignana Vol-II, chapter 4th, 1st edition, Varanasi:
Choukhamba Bharateeya Academy, 1981, , p. 275.
b. K.M. Nadakarani, Indian Materia Medica Vol - I, editor A. K. Nadakarni, 3rd
edition, Bombay: Popular Prakashana Private Limited, 1982, p. 965.
c. JLN Shastri, Dravyaguna Vignana Vol - II, 1st edition, Varanasi:
Choukhamba Orientalia, 2004, P. 452.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 107
Bibilography
99. a .P.V. Sharma, Dhanwantari Nighantu, chapter 2nd, shloka 73-74, editor
Guruprasada Sharma, 1st edition, Varanasi : Choukhamba Orientalia, 1982, ,
p. 83.
b. Pandita Narahari, Rajanighantu, Pippalyadi varga, shloka 11-13, edited
by Indradeva Tripathi, 2nd edition, Varanasi : Krishnadasa Academy, 1998, , p.
135-136.
c.Bhavamishra, Bhavaprakasha nighantu, Haritakyadi varga, shloka 55-
58, edited by G.S. Pandye, 7th edition, Varanasi: Choukhamba Bharateeya
Academy, 1984, p. 15.
d. Madana Pala, Madanapala nighantu, Shyunthyadi varga, shloka 12,
Mumbai: Kemaraja Shri. Krishnadasa Prakashana, 1988, p. 66.
e. P.V. Sharma, Kaiyadeva Nighantu, Oushadhi varga, shloka 1165-
11691st edition, Varanasi : Choukhamba Orientalia, 1989, , p. 215.
f. K.M. Nadakarani, Indian Materia Medica Vol-I, editor A. K.
Nadakarni, 3rd edition, Bombay: Popular Prakashana Private Limited, 1982,
p.965.
100. Bhava mishra Bhava prakash Nighantu, Karpuradi Varga ,edited by G.S.
Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy, 1984.P.218.
101. JLN Shastri, Dravyaguna Vignana Vol-II, 1st edition, Varanasi:
Choukhamba Orientalia, 2004, p. .
102. Bhava mishra Bhava prakash Nighantu, Karpuradi Varga ,sloka 57,edited by
G.S. Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy, 1984.P.218.
103, a. Bhava mishra Bhava prakash Nighantu, Karpuradi Varga, edited by G.S.
Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy, 1984.P.218.
b. Kaiyadeva, Kaiyadeva Nighntu, Oushadi Varga, Sloka 1329-1330,edited
by P.V.Sharma, Ist edition, Varnasi , Chaukamba Orientalia, 1979. P.246.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 108
Bibilography
c. P.V.Sharma, Dhanwantari Nighantu, ChandadiVarga, Sloka 31-32, edited
by Guruprasad Sharma, 3rd ed. Varnasi Chaukamba Orientalia, 2002. P.96.
d. Madanapala, Madanapla Nighantu,- karpuradivarga, sloka 20, edited by
Kemaraja, Mumbai, Sri Krishnadas Prakashan, 1998. P.80.
e. Pandit Narahari, Raja Nighantu, Chandanadi Varga, Sloka 75,edited by
Indradev Tripati , 2nd ed, Varanasi , Krishnadasa Academy, 1998. P.410.
104. a. Bhava mishra Bhava prakash Nighantu, Karpuradi Varga,sloka 57, edited by
G.S. Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy, 1984.P.218.
b. Kaiyadeva, Kaiyadeva Nighntu, Oushadi Varga, Sloka 1329-1330, edited by
P.V.Sharma, Ist edition, Varnasi , Chaukamba Orientalia, 1979. P.246.
c. P.V.Sharma, Dhanwantari Nighantu, Chandanadi Varga, Sloka 31-32,
edited by Guruprasad Sharma, 3rd ed. Varnasi Chaukamba Orientalia, 2002. P.96.
d. Pandit Narahari, Raja Nighantu, Chandanadi Varga, Sloka 76, edited by
Indradev Tripati , 2nd ed, Varanasi , Krishnadasa Academy, 1998. P.410.
105. a. Bhava mishra Bhava prakash Nighantu, Karpuradi Varga,sloka 57, edited by
G.S. Pandye, 7th ed., Varnasi Chaukamba Bharatiya Academy, 1984.P.218.
b. Kaiyadeva, Kaiyadeva Nighntu, Oushadi Varga, Sloka 1329-1330,edited by
P.V.Sharma, Ist edition, Varnasi , Chaukamba Orientalia, 1979. P.246.
c. P.V.Sharma, Dhanwantari Nighantu, Chandanadi Varga, Sloka 31-32, edited
by Guruprasad Sharma, 3rd ed. Varnasi Chaukamba Orientalia, 2002. P.96.
d. Madanapala , Madanapla Nighantu,- KarpuradiVarga, sloka 20 , edited by
Kemaraja , Mumbai, Sri Krishnadas Prakashan, 1998. P.80.
e. Pandit Narahari, Raja Nighantu, Chandanadi Varga, Sloka 76, edited by
Indradev Tripati , 2nd ed, Varanasi , Krishnadasa Academy, 1998. P.410.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 109
Bibilography
106. Sri Sadananda Sharma, Rasa Tarangini, 2nd Chapter, sloka 52.,edited by Kashinatha Shastri, 11th Edition, New Delhi, Motilala Banarasidas publication, 1979.P.22. 107. Sri Harisharananda, Bhasma Vvijana, Ayurveda Vijnana Grautha Karyalaya, Punjab, 1st Edition 2000, P.12, 13, 32, 33. . 108. Acharya Sharangadhara, Sharangadhara Samhita Madhyama Khanda 12th Chapter, Sloka 16-17,edited by Dr. Brahamananda Tripati, 3rd edition, Varanasi, Chaukamba Oriential, 1996.P.261.
“Pharmaceutico-Analytical study on Swacchandda Bhairava rasa (Dwitiya)” 110