t c c a t a t c a t c a t c c a t a t ... - fisher scientific...c a t a t c a t c a t c a t c a t c...

G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C

G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T

PCR and qPCR solutions•IndustryleadingPCRandqPCRreagents•Trustedplasticconsumables•HighqualityPCRandqPCRinstruments

Industry leading PCR enzymes and kits

Do you neeD a hIgh-fIDelIty PCR enzyme wIth a veRy low eRRoR Rate?

NO

Do you need increased specificity and/or do you set up PCR reactions in a room temperature?

Do you need increased specificity and/or do you set up PCR reactions in a room temperature?

Do you amplify very long (>20 kb) targets?

Would you benefit from faster cycling and higher yields?

Is dutP incorpo-ration required in your PCR?

• long PCR enzyme mix*

YESNO

YESNO

YES

• maxima hot Start taq Dna Polymerase

• maxima hot Start green PCR master mix

• Phire hot Start II Dna Polymerase

YESNO

would you like to have a colored polymerase that enables direct loading of PCR product to the gel?

• taq Dna Polymerase

• PCR master mix

YESNO

• Dreamtaq Dna Polymese

• Dreamtaq PCR master mix

• Dreamtaq green Dna Polymerase

• Dreamtaq green PCR master mix

YESNO

Do you need very fast cycling times for time savings?

• Phusion Hot Start II high-fidelity Dna Polymerase

YESNO

• Phusion High-Fidelity Dna Polymerase

• Phusion High-Fidelity PCR master mixes

• Phusion Flash high-fidelity PCR master mix

YESNO

PCRThermo Scientific multiple polymerases and master mixes deliver top performance over a range of applications, from Standard PCRto high-fidelity and Direct PCR.

Best-in-class high-fidelity Dna Polymerases – Phusion Dna Polymerases• Highlyaccurateamplification• ShortPCRprotocols• Minimaloptimization• SpecificPCRproducts• Availablealsoashotstartandfastmastermixformulations

www.thermoscientific.com/phusion

Shortcut to PCR – Thermo Scientific Direct PCR Kits• NoDNApurificationneeded,sampleaddeddirectlytoPCRmix• Significantsavingsintimeandcost• Simpleprotocols,minimalhands-ontime• Forplantandanimaltissues,humanspecimenandblood

www.thermoscientific.com/directpcr

CHOOSE THE BEST ENZYME FOR YOUR APPLICATION

* NotavailableinUS.DIRECT PCR – a new ShoRtCut to faSt ReSultS!

everything for PCR and qPCRThe Thermo Scientific PCR and qPCR portfolio has everything you need for a successful run: reagents, instruments and plastic consumables. our innovative portfolio is assembled by combining the state-of-the-art product lines from aBgene, finnzymes and fermentas. the within – themselves born of scientific breakthroughs – are accelerating discovery in life science, and our aim is to continue to develop new tools to increase your efficiency and enhance your effectiveness.

high quality for fast and Conventional PCRThermo Scientific Piko and Arktik Thermal Cyclers • High-qualityPiko®ThermalCyclersrepresentthenextgenerationofPCRinstrumentation.

ThePikoThermalCyclerisbasedaroundthe24-welland96-wellPikoPCRPlate.• Arktik™ThermalCyclerisareliableandflexiblePCRinstrumentfortheeveryday

requirementsofanylaboratory.Itissuitableforawiderangeofapplicationsfromindividualreactionstohigh-throughputprojects.

www.thermoscientific.com/piko,www.thermoscientific.com/arktik

Real-time. any time. now within your reach.Thermo Scientific PikoReal Real-Time PCR System*• ThePikoReal®Real-TimePCRSystemisdesignedtofitintoyourlab,evenwhenbench-

topspaceislimited.OfferinganexceptionallysmallfootprintthisqPCRinstrumentisidealforpersonaluseandlightenoughforfieldapplications.ThePikoRealSystemalsodeliversinnovativefeaturesandoutstandingperformance.

www.thermoscientific.com/pikoreal

Complete range of plastic consumablestubes • Individualtubesandstriptubes

Plates• 24,48,96and384-wellplatesandPikoUTWPCRPlates

Sealing options• CapStrips,AdhesiveSealsandStoragePlateSealing

Storage plates• 48-Well,96-Welland384-WellStoragePlates

Barcoding• Reliablesampletracking

www.thermoscientific.com/pcrplastics

high-quality instruments & plastic consumables

www.thermoscientific.com/pcr

PikoThermalCycler

* NotavailableinCanada,Brazil,UK,Germany,Austria,Switzerland,Italy,Spain,France,Belgium,theNetherlands,Liechtenstein,DenmarkandSweden.

Complete selection of qPCR reagents

qPCRour qPCR reagents have been engineered to give maximum sensitivity and reproducibility. you can choose optimized master mixes for both fast and standard protocols, and minimize pipetting errors by choosing colored reagents. the pre-designed qPCR gene-expression assays offer convenient a solution for gene-specific quantification with a single assay.

CHOOSE YOUR DETECTION CHEMISTRY

PRe-DeSIgneD PRoBe-BaSeD qPCR aSSayS PRoBe SyBR gReen

gene expression assays for human and mouse genes

Standard qPCR master mixes

fast qPCRmaster mixes

Standard qPCR master mixes

fast qPCRmaster mixes

• Solaris qPCR Gene expression assays - gene specific primers and probe

• Solaris qPCR Master Mix

• Maxima Probe master mixes

• DyNAmo Flash and Colorflash qPCR Kits for probe chemistry

• Maxima SYBR green master mixes

• DyNAmo Flash and Colorflash qPCR Kits for SYBR green chemistry

Thermo Scientific Maxima qPCR Master Mixes for standard qPCR• Specificity–MaximaHotStartTaqplusoptimizedbuffereliminatesnonspecific

amplification/formationofprimerdimers• Sensitivity–detectslowcopynumbertargets• Widelinearrange–accuratequantificationacrossnineordersofmagnitude

Thermo Scientific DyNAmo Flash Kits for fast qPCR • Extremelyfastprotocols–combinedannealingandextensionstepofonly15s• Specificityandsensitivity–detectionofawiderangeoftemplateconcentrations

Thermo Scientific DyNAmo ColorFlash Kits for fast qPCRAllthebenefitsofDyNAmoFlashKits,plus:• Multi-colorreagentsystem(bluemastermix,yellowsamplebuffer)• Minimizedriskofpipettingerrorsduringreactionsetup• Especiallybeneficialwhenusingwhitereactionvessels

Thermo Scientific Solaris qPCR Gene Expression Assays - for human and mouse genomes • Pre-designedMGB-probeandprimerassays–minimaloptimizationrequirements• Superbaseincorporation–oneoptimaldesignpergenetargetdetectsallsplicevariants

www.thermoscientific.com/qpcrsolution

Thermo Scientific Maxima First Strand cDNA Synthesis Kit for RT-qPCR• Sensitiveandreproducible–cDNAsynthesisfromawiderangeoftotalRNAamounts(1pg-5μg)• Increasedsynthesisrates–completecDNAsynthesisin15-30minutes• Highyields–fulllengthcDNAupto20kbinlength• Increasedreactiontemperatures–activeupto65°C• Increasedreproducibility–reducedbias

RevertAid Premium First Strand cDNA Synthesis Kit for cloning and difficult templates*• Increasedreactiontemperatures–thefirststrandofcDNAcanbesynthesizedwithinthe50-65°C

temperaturerange• Highyieldsoffull-lengthfirststrandcDNAwithRNAtemplatesupto20kb• Flexiblepriming–oligo(dT)18,randomhexamerorgene-specificprimers

Complete kits for Rt-PCR and Rt-qPCR• Verso1-StepKitsforRT-qPCR -ForbothSYBRGreenandprobechemistries• Verso1-stepRT-PCRKits -Standardandhotstartenzymeformulations -RTenhancerincluded• PhusionRT-PCRKit -Complete2-stepkitforhigh-fidelityRT-PCR

www.thermoscientific.com/rt

enhanced solutions for reverse transcription

RTThermo Scientific products offer multiple solutions for reverse transcription for both PCR and qPCR applications.

CHOOSE THE BEST RT ENZYME FOR YOUR APPLICATION

RoutIne PeRfoRmanCe enhanCeD PeRfoRmanCe

aPPlICatIonS Revertaid Reverse transcriptase1

Revertaid h minus Reverse transcriptase2

Revertaid Premium Reverse transcriptase3

maxima Reverse transcriptase

Rt-PCR up to 5 kb4 •••• •••• ••••• •••••

Rt-PCR 5-20 kb4 •• ••• ••••• •••••

full lenght cDna 5-20 kb •• ••• ••••• •••••

cDna labeling •• ••• ••••• ••••

RaCe/Primer extension •5 ••••• ••••• ••5

1 not available in Canada2 not available in uS3 not available in uS and Canada4 at their optimal temperatures5 not for RaCe

Available as enzyme kits and first strand cDna synthesis kits.

*NotavailableinUSandCanada.InthesecountrieswerecommendMaximaUniversalFirstStrandcDNASynthesisKit.

G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C

G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T A T C G A T C G A T C G C G A T G C G A T A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T C G A T

BN0110126

PikoThermalCycler,ArktikThermalCyclerandPikoRealRealTimePCRSystemarelicensedunderU.S.PatentNos.5,552,580and5,496,517.

PikoThermalCyclerandArktikThermalCycler:Purchaseofthisinstrumentconveysalimited,non-transferableimmunityfromsuitforthepurchaser'sowninternalresearchanddevelopmentandappliedfieldsotherthanhumaninvitrodiagnosticsunderoneormoreofCanadianPatent1,339,653,U.S.Patent5,475,610(claims160-163only)orcorrespondingclaimsintheirunexpirednon-U.S.counterpartsownedbyAppliedBiosystems.

PikoRealRealTimePCRSystem:Purchaseofthisinstrumentconveysalimited,nontransferableimmunityfromsuitforthepurchaser'sowninternalresearchanddevelopmentandappliedfieldsotherthanhumaninvitrodiagnosticsonlyunderCanadianPatent1,339,653,U.S.Patent5,475,610(claims160-163only)andnon-U.S.counterpartclaimsasapplicable.

©2012ThermoFisherScientificInc.Allrightsreserved.AlltrademarksarethepropertyofThermoFisherScientificInc.anditssubsidiaries.

In the United States:For customer service, call 1-800-766-7000.To fax an order, use 1-800-926-1166.To order online: www.fishersci.com

In Canada:For customer service, call 1-800-234-7437.To fax an order, use 1-800-463-2996.To order online: www.fishersci.ca















Forfurtherinformationvisitwww.thermoscientific.com/pcr

Technical Support:

North [email protected]

Ordering information:

United StatesForcustomerservice,call1-800-766-7000.Tofaxanorder,use1-800-926-1166.Toorderonline:www.fishersci.com

CanadaForcustomerservice,call1-800-234-7437.Tofaxanorder,use1-800-463-2996.Toorderonline:www.fishersci.ca

t c c a t a t c a t c a t c c a t a t ... - fisher scientific...c a t a t c a t c a t c a t c a t c...

Documents