TDM OF DIGOXIN

By,

Surya Prakash singh

ROLL: 12117D3003

pharmD IV th year

IT'S A CARDIAC GLYCOSIDETHE SOURCE'S ARE DIGITALIS LANNATA DIGITALIS PURPUREAITS STRUCTURE

Basic cardenolide with three digitoxose untis bound to streroidal ringby a glycosidic linkage.

DRUG PROFILE OF DIGOXIN

DOSE AND INDICATION

DOSE:

Tablet : 0.125 - 0.25 mg TABInjection solution : 0.1mg/ML - 0.25mg/ML

INDICATION:

heart failure & atrial fibrillation.

ABSORPTION

BA: 55-90%ONSET OF ACTION PO : WITHIN 2 HR IV : WITHIN 30 MINDURATION OF ACTION : 3-4 DAYSPEAK PLASMA TIME : 1 HR

DISTRIBUTIONPROTEIN BOUND: 20-25% VD : 4 – 7 L/KGMETABOLISMLIVERELIMINATIOINT 1/2 : 1-3 DAYS EXCRETIONURINE : 80%FEACES: 10-15%

MECHANISM OF ACTION OF DIGOXIN

OTHER EFFECTS :

Neuro hormonal effects• Decrease sympathic out flow• Increase parasympathetic outflow• Improve baro receptor functioning

DECREASE HEART RATE

(NEGATIVE CHRONOTROPIC

EFFECT)

REASONS FOR TDM OF DIGOXIN ??????

METHOD OF ASSAY

Radioimmunoassay (RIA) involves the quantitation of drug using a radioactive probe by measuring the radioactivity correlated to amount of drug.The technique was introduced in 1960 by Berson and Yalow

Mixture of radioactive labeled antigen "hot"

+ antibody

Adding the sample unlabeled antigen (drug)"cold"

The unlabelled antigen displaces the labeled ones

(Ag-Ab)Complexes are removed

Supernatant remains are analyzed

Gamma Counter

From these data, a standard binding curve, like the one shown in red, can be drawn.

OTHER METHODS OF ASSAYS

Nucleic acid labeling Fluorescence labeling ELISA High throughput screening wells coated with

antibodies.

THANK YOU