the 12 hasib 2019 - unissula

Proceeding of International Halal Science and Technology Conference (IHSATEC)

The 12th HASIB 2019

VOLUME 1

Bangkok International Trade and Exhibition Centre (BITEC)

Bangkok, Thailand

December 20-22, 2019

978-616-407-582-5

PREFACE

The Halal Science Center Chulalongkorn University (HSC-CU) in collaboration with the Central Islamic Council of Thailand (CICOT) and the Halal Standard Institute of Thailand (HSIT) hosted the 6th Thailand Halal Assembly 2019 between 20-22 December, 2019 at the Bangkok International Trade and Exhibition Center (BITEC) in Bangkok, Thailand under the main theme “Algorithmic Touch of Halal.” The 12th Halal Science, Industry and Business International Conference 2019 (12th HASIB 2019) and the International Halal Science and Technology Conference (IHSATEC), reflecting the systematic integration of standardization, science, technology, and innovation into the Halal process to be able to verify each operational procedure with precision in connection with information technology to increase global competitiveness. The purpose of the HASIB Conference was to highlight significant developments in Halal Science, Technology and Innovation for researchers, graduate students, and practitioners to exchange information on contemporary research findings and emerging issues, and to discuss and collaborate in exploring opportunities for Halal Industry. The Proceedings comprise 13 academic papers from different fields relating to Halal issues reviewed by the Editorial Board of the Organizing Committee. They are divided into five sessions corresponding to five different tracts of the Conference, demonstrating a wide range of topics and covering almost all areas in Halal Science, Technology and Innovation. In addition, issues related to Halal marketing, business, and industry are also addressed in line with the theme of the Conference. The organizing Committee wishes to thank the authors of the papers, the reviewers, and the HSC-CU staff who have rendered their dedication and enthusiasm to the success of this productive and memorable Conference.

Associate Prof. Dr. Winai DahlanChairman of the Organizing CommitteeThailand Halal Assembly 2019Editor of 12th HASIB 2019 Proceedings

Conference Organizers The Halal Science Center Chulalongkorn University (HSC-CU), Thailand

Local Organizing Committee Dr. Pornpimol Mahamad Dr. Acharee Suksuwan

Local Organizing Staff Ms. Nureesun Mahamad Ms.NajwaYanyaSantiworakun Ms. Kasinee Katelakha Ms. Sunainee Mahama Ms. Baddariyah Sohsansa Ms. Nareeya Waloh Ms. Kunthira Salae Ms. Angsana Ayukhen Ms. Netnapa Ontao Ms. Saveeyah Kahong

Scientific Committee - Committee Assoc. Prof. Dr. Winai Dahlan - Vice Chairman Assist. Prof. Dr. Vanida Nopponpunth - Committee Prof.Dr.MosaadAttiaAbdel-Wahhab Prof. Dr. Hamzah Mohd Salleh Prof. Dr. Irwandi Jaswir Prof. Dr. Nazimah Hamid Prof. Dr. Shuhaimi Mustafa Assist. Prof. Nifarid Raden-ahmad Assist. Prof. Dr. Suwimol Sapwarobol Assist. Prof. Dr. Sathaporn Ngamukote Assist. Prof. Dr. Sukrit Sirikwanpong Assist. Prof. Dr. Tewarit Sarachana Dr.HendratiDwiMulyaningsih Dr. Nor Amin Modh Noor Dr. Pornpimol Mahamad Dr. Acharee Suksuwan Dr. Pakpum Somboon

Business & Marketing Committee - Chairman Assoc. Prof. Dr. Pakorn Priyakorn - Vice Chairman Assist. Prof. Dr. Pradorn Sureephong - Committee Prof. Dr. Faridah Hj Hassan Assist. Prof. Dr. Sarawut Aree Dr. Hendri Tanjung Mr. Bouaoud Malik

Proceedings of International Halal Science and Technology Conference(IHSATEC)


EDITORIAL BOARD OF THE PROCEEDINGSChairman

Assoc. Prof. Dr. Winai Dahlan Thailand

Vice Chairman

Assist. Prof. Dr. Vanida Nopponpunth Thailand

Committee

Prof.Dr.MosaadAttiaAbdel-Wahhab Egypt Prof. Dr. Faridah Hj Hassan Malaysia Prof. Dr. Hamzah Mohd Salleh Malaysia Prof. Dr. Irwandi Jaswir Malaysia Prof. Dr. Nazimah Hamid New Zealand Prof. Dr. Shuhaimi Mustafa Malaysia Assoc. Prof. Dr. Pakorn Priyakorn Thailand Assist. Prof. Nifarid Raden-ahmad Thailand Assist. Prof. Dr. Pradorn Sureephong Thailand Assist. Prof. Dr. Suwimol Sapwarobol Thailand Assist. Prof. Dr. Sathaporn Ngamukote Thailand Assist. Prof. Dr. Sarawut Aree Thailand Assist. Prof. Dr. Sukrit Sirikwanpong Thailand Assist. Prof. Dr. Tewarit Sarachana Thailand Dr.HendratiDwiMulyaningsih Malaysia Dr. Hendri Tanjung Indonesia Dr. Nor Amin Modh Noor Malaysia Dr. Pakpum Somboon Thailand Dr. Pornpimol Mahamad Thailand Dr. Acharee Suksuwan Thailand Mr. Bouaoud Malik Qatar

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TABLE OF CONTENTS

SESSION: Halal Science Technology & Innovation Research • Cholesterol-lowering agent and pigments production from Hom Thong banana 9 peels by Monascus purpureus LalitaKhacharat,WarapaMahakarnchanakul,UdomlakSukatta,PrapassornRugthaworn, Ketsaree Klinsukhon, Thanapoom Maneeboon

• Development of Organic Soap from Brown Rice Germs in Pattani 16 LadawanKhongsichan

• Evaluation of Lozenge Tablet from the Ethanol Extract of Miswak (Salvadora persica L.) 21 as an Innovation for Mouth Cleaning NadiaMiraKusumaningtyas,IrtizaqunNabila,AndiSriSuriatiAmal, Amal Fadholah, Nurul Marfu’ah

• Attitude toward Halal, Religiosity, and Intention toward Measles Rubella 28 Immunization among Mothers Attending Public Health Center in Semarang City, Indonesia ChilmiaNurulFatiha,WilliWahyuTimur

• The Development of Selective Courses on Halal Science and Technology 33 for Secondary School Students HabillaChapakiya,NifaridRadenahmad,AsraPohwoh,AnyamaneeNakkarakel

• Analgesic Effect of Garlic Peel Extract (Allium Sativum, L) on Mice (Mus Musculus) 39 RinaWijayanti,NisaFebrinasari

• Accelerated Shelf Life Determination of Bastard Oleaster Sheets 43 RatchaneewanKulchan,PanidaBanjongsinsiri,BussakornPraditniyakul, Wisanee Nuamekin, Bunrit Phraelai

• The Effects of Temperature, Autoclaving Condition, pH and Light on The Stability 55 of Anthocyanin from Coconut (Cocos Nucifera) Husk AriyaniRinaldi,RodiahMohdHassan

• The Application of Umum Balwa Analysis Tool (UBAT) in Managing 63 Cross-Contamination of Najs towards Halal Food Product MuhamadAfiqAbdRazak,MohdAnuarRamli

• Can the Halal Grill Lamb Meat Intake Increase the Blood Glucose? 73 Fathimah,KartikaPibriyanti,FitriKomalaSari,HafidhotunNabawiyah,MutiaYuliza

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TABLE OF CONTENTS

SESSION: Halal Science Technology & Innovation Research • Cholesterol-lowering agent and pigments production from Hom Thong banana 9 peels by Monascus purpureus LalitaKhacharat,WarapaMahakarnchanakul,UdomlakSukatta,PrapassornRugthaworn, Ketsaree Klinsukhon, Thanapoom Maneeboon

• Development of Organic Soap from Brown Rice Germs in Pattani 16 LadawanKhongsichan

• Evaluation of Lozenge Tablet from the Ethanol Extract of Miswak (Salvadora persica L.) 21 as an Innovation for Mouth Cleaning NadiaMiraKusumaningtyas,IrtizaqunNabila,AndiSriSuriatiAmal, Amal Fadholah, Nurul Marfu’ah

• Attitude toward Halal, Religiosity, and Intention toward Measles Rubella 28 Immunization among Mothers Attending Public Health Center in Semarang City, Indonesia ChilmiaNurulFatiha,WilliWahyuTimur

• The Development of Selective Courses on Halal Science and Technology 33 for Secondary School Students HabillaChapakiya,NifaridRadenahmad,AsraPohwoh,AnyamaneeNakkarakel

• Analgesic Effect of Garlic Peel Extract (Allium Sativum, L) on Mice (Mus Musculus) 39 RinaWijayanti,NisaFebrinasari

• Accelerated Shelf Life Determination of Bastard Oleaster Sheets 43 RatchaneewanKulchan,PanidaBanjongsinsiri,BussakornPraditniyakul, Wisanee Nuamekin, Bunrit Phraelai

• The Effects of Temperature, Autoclaving Condition, pH and Light on The Stability 55 of Anthocyanin from Coconut (Cocos Nucifera) Husk AriyaniRinaldi,RodiahMohdHassan

• The Application of Umum Balwa Analysis Tool (UBAT) in Managing 63 Cross-Contamination of Najs towards Halal Food Product MuhamadAfiqAbdRazak,MohdAnuarRamli

• Can the Halal Grill Lamb Meat Intake Increase the Blood Glucose? 73 Fathimah,KartikaPibriyanti,FitriKomalaSari,HafidhotunNabawiyah,MutiaYuliza

• Development of a DNA Biosensor Using Screen-Printed Gold Electrode Modified 80 by Electrodeposition of Polyaniline-Graphene-Silver Nanocomposite OrawanThipmanee

• Effect of Packaging Films on Quality of Cereal Snack Bar during Storage 87 BussakornPraditniyakul,RatchaneewanKulchan,WisaneeNeumakin, WichitRattanathawornkiti

SESSION: Halal Business & Marketing Research • Sharia Governance Implementation in Islamic Banking Windows of Regional 94 Development Bank in Indonesia FajarAmtsal,ElyWindartiHastuti

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Analgesic Eeffect of Garlic Peel Extract (Allium sativum, L)

on Mice (Mus musculus)

Rina Wijayanti1*#, Nisa Febrinasari1 1Departement of Pharmacy, Faculty of Medicine, Universitas Islam Sultan Agung,

Kaligawe Street KM 4 Semarang, Central Java, Indonesia 50012 Telp (+6224) 6583584 Fax (+6224) 6582455

*#Corresponding author : [email protected]

Abstract

Pain is the body's protective mechanism against disruption and damage of tissue such as inflammation, microorganism infection, muscle spasms with the release of pain mediators namely prostaglandin, bradykinin, serotonin, histamine, potassium ions, and acetylcholine. Previous research concluded that garlic peel contains flavonoids. The purpose of this study was to determine the analgesic effect of peel extract on mice. Thirty five test Animals (20-40 g) were divided into 5 groups. Group I was the negative control group (aquadest), group II was the positive control group (paracetamol), groups III, IV, and V were treated orally with garlic peel extracts at a dose of 150, 300 and 600 mg/kg b.w, respectively. Before testing was performed, mice were fasted for about 11 hours then weighed. The mice were induced thermally then the response was observed for 1 minute. Observation was carried out 5 times, namely: before administering the test substance, at 30, 60, 90, and 120 minutes after the administration of the test substance. Data analysis by using Mann Whitney non-parametric test showed that the analgesic effect of garlic peel extract began to be seen at 30-th, 60-th, 90-th, and 120-th minutes. The average difference of righting reflex response of mice showed that the strongest analgesic effect at a dose of 600 mg/kg b.w. It could be concluded that garlic peel extract has analgesic effect on mice. Keywords: Analgesic effect, garlic peel extract.

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Introduction

Pain is the body's protective mechanism against disruption and damage of tissue such as inflammation, microorganism infection, muscle spasms with the release of pain mediators namely prostaglandin, bradykinin, serotonin, histamine, potassium ions, and acetylcholine (Tjay and Rahardja, 2002). In general, diseases of the body cause pain. Pain is a defensive function which allows for immobilization of body organs that are inflamed or broken so that sensation perceived will subside and can speed healing, pain also functions as a diagnostic guide, because with pain in certain areas, the process that occurs in patients can be known (Irianty et al., 2014). The International Association for the Study of Pain (IASP) defined pain as an unpleasant sensory and emotional experience associated with the occurrence of tissue damage both actual and potential (IASP, 2011; Xie, 2011). Chronic pain is a serious problem due to the high prevalence rate. Chronic pain is one of the most frequently reported symptoms in one in six people in the population and is estimated to occur in 2-40% of the adult population (Mallen et al., 2005; Xie, 2011). In Indonesia, in the elderly population, it is reported that 25-50% of them experience pain (Kartini, 2007). Chronic pain condition has an impact on increasing health costs (Mallen et al., 2005). In general, people consume analgesic drugs, both opioid and non-opioid groups. However, the use of these drugs often cause side effects especially in the long term (Irianty, 2014). In addition to having an impact on increasing health costs and side effects caused by synthetic drugs, the use of medicines from nature needs to be studied more deeply. Garlic is used as a spice in cooking as well as a natural treatment. Research conducted by Wijayanti and Rosyid (2015) concluded that garlic peel contains flavonoids. The results of research conducted by Sibarani et al. (2014) proved that the content of flavonoids in the leaves of camphorweed (Pluchea indica) at a dose of 150 mg/kg of body weight, 300 mg/kg of body weight and 600 mg/kg of body weight had analgesic effects. In an effort to utilize garlic peel waste and as an alternative treatment for pain, further research needs to be performed on the analgesic activity of garlic peel extract in mice. The purpose of this study was to determine the analgesic effect of peel extract on mice. Research Method

This research was experimental with post test only control group design. The sample in this study was 20-40 g female mice (Mus musculus) that were randomly selected as many as 5 animals per group. The tools used in this study were a set of tools for extraction, beaker glass, timer, 1 ml syringe, NGT no 3.5, and thermometer. The ingredients used in this study were the garlic peel, 70% ethanol, 500 mg paracetamol tablets, aquadest, and pellets. The making of garlic peel extract was performed by maceration method, which is the dried garlic peel then sifted, weighed as much as 120 g then extracted using 900 ml of 70% ethanol by maceration for 5 days (every day shaken), then the extract was filtered by using filter paper (filtrate 1) and the rest was extracted again for 2 days then filtered using 70 ml of ethanol as much as 300 ml (filtrate 2). Furthermore, filtrate 1 and 2 were collected, evaporated by using vacuum evaporator at a temperature of 70 oC to ¼ from the initial volume, and continued with drying on a water bath at 60 oC until it became a thick extract (Wijayanti and Rosyid, 2015). Thirty five test animals were divided into 5 groups. Group I was the negative control group, group II was the positive control group, groups III, IV, and V were the treatment groups by administering garlic peel extracts with a dose of 150 mg/kg of body weight, 300 mg/kg of body weight, and 600 mg/kg of body weight. Before testing was performed, mice were fasted for 11 hours then weighed. Beaker was put into a water bath filled with water, then the water bath was heated to a temperature of 55 °C. After the temperature reached 55 °C, the mice were put into the beaker glass. After the mice were in the beaker glass, the response was observed, which is in the form of licking the feet and or

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jumping. Observation was carried out for 1 minute. To each group of mice were given an extract solution, a comparative drug solution (paracetamol) as a positive control, and aquadest as a negative control. Mice then rested to be observed again in the 30th minute. Observations were made until the 120th minute, with intervals of 30 minutes for each observation. Observation was carried out 5 times, namely: before administering the test substance, 30-th, 60-th, 90-th, and 120-th minutes after administering the test substance (Sibarani, 2014). Results and Discussion

The results of the research are presented in Figure 1. below:

Figure 1. Average difference graph of righting reflex response of mice

The results of the difference in the righting reflex of mice in each subsequent minute were analyzed, and the results obtained were abnormal and non-homogeneous data, so the analysis was continued by using Kruskal Wallis non-parametric test, and obtained significance of 0.000 and continued using the Mann Whitney test. Analgesic effects can be demonstrated by the reduction in the number of mice responses, or the greater the difference in righting reflex of mice, the greater the analgesic effect shown. Test results on the extract group showed that there was a decrease in response or increase in the difference in the response of the righting reflex of mice. This showed that garlic peel has an analgesic effect. The analgesic effect of garlic peel extract is assumed to be due to the role of flavonoids. This is in line with research conducted by Sibarani, et al. (2013) which concluded that camphorweed leaf extracts showed analgesic effect. The analgesic effect of garlic peel extract began to appear at 30-th, 60-th, 90-th, and 120-th minutes. On the average difference graph of righting reflex response of mice showed that the strongest analgesic effect was at a dose of 600 mg/kg of body weight. This showed that the dose of 600 mg/kg of body weight is the maximum dose because the dose has reached the maximum therapeutic level. The ability of garlic peel extract as an analgesic was higher than that of paracetamol.

0:00:00

48:00:00

96:00:00

144:00:00

192:00:00

240:00:00

288:00:00

DELTA30

DELTA60

DELTA90

DELTA120

Diffe

renc

e in

the

resp

onse

of t

he

right

ing

refle

x of m

ice

Minutes after the administration of the test substance

Garlic peel extract600 mg/kg BW



POSITIVE

NEGATIVE

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Based on the results obtained through testing in the positive control group with paracetamol, showed a decrease in the average response of test animals to pain stimulation (Sibarani, 2013). The negative control group showed significant differences in the garlic peel extract group dosage of 150 mg/kg of body weight, 300 mg/kg of body weight, and 600 mg/kg of body weight at 30-th minutes; and at a dose of 300 mg/kg of body weight and 600 mg/kg of body weight at 60-th minute. This showed that the garlic peel has analgesic activity. The ability of the extract to reduce pain response is due to its flavonoid content. The working mechanism of flavonoids is to inhibit the work of the cyclooxygenase enzyme, thereby reducing the production of prostaglandins by arachidonic acid so that it reduces pain, besides flavonoids also inhibit neutrophil degranulation so that it will inhibit the release of cytokines, free radicals, and enzymes that play a role in inflammation (Sentat and Pangestu, 2016). Conclusion

Garlic peel extract has analgesic effect on mice at a dose of 600 mg/kg b.w.

Reference

International Association for the Study of Pain (IASP), 2011.IASP Taxonomy, http://www.iasp-pain.org [diakses tanggal 15 Juni 2015]

Irianty, C. R., Posangi, J., Wowor, P. M., 2014, Uji Efek Analgesik Ekstrak Etanol Kelop[ak Bunga Matahari (Helianthus annuus Linn.) pada Mencit Swiss (Mus musculus), Jurnal e-Biomedik (eBM) , Volume 2 Nomor 2 Juli 2014, Fakultas Kedokteran Unsrat

Kartini, 2007. Hubungan Nyeri dengan Gangguan Aktivitas Interpersonal pada Individu Usia 50 Tahun Keatas di Kabupaten Purworejo. Yogyakarta: Universitas Gadjah Mada

Mallen, C., Peat, G., Thomas, E., and Croft, P., 2005. Severely Disabling Chronic Pain in Young Adults : Prevalence from a Population-based Postal Survey in North Staffordshire, BMC Musculoskeletal Disorders, 42(6) : 1-9

Sentat, T., Pangestu, S., 2016, Uji Efek Analgesik Ekstrak Etanol Daun Kersen (Muntinga calabura L.) pada Mencit Putih Jantan (Mus musculus) dengan Induksi Nyeri Asam Asetat, Jurnal Ilmiah Manuntung, 2(2) : 147-153

Sibarani, V. R., Wowor, P. M., Awaloei, H., 2014, Uji Efek Analgesik Ekstrak Daun Beluntas (Pluchea indica (L.) Less.) pada Mencit (Mus musculus), Jurnal e-Biomedik (eBM), Volume 2 Nomor 2 Juli 2014, Fakultas Kedokteran Unsrat

Tjay, T. H dan Rahardja, K., 2002, Obat-obat Penting : Khasiat, Penggunaan dan Efek-efek Sampingnya, Ed. 5, Jakarta : Elex Media Komputindo Kelompok Gramedia

Widodo, D. S., 2011, Perbandingan Efektivitas Antara Ketorolak dan Petidin Sebagai Obat Anti Nyeri Pascaoperasi, Skripsi, Fakultas Kedokteran Universitas Sebelas Maret, Surakarta

Wijayanti, R., Rosyid, A., 2015, Efek Ekstrak Kulit Umbi Bawang Putih (Allium sativum L.) terhadap Penurunan Kadar Glukosa Darah pada Tikus Putih Jantan Galur Wistar yang Diinduksi Aloksan, Jurnal Ilmu Farmasi & Farmasi Klinik, Vol 12 (1).

Xie, W., 2011. Assessment of Pain in Animals In Ma, C., and Zhang, J.M. (Eds). AnimalModels of Pain, New York : Humana Press, p. 23-76

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