thomas raymond unnasch distinguished usf health …
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Thomas Raymond Unnasch Distinguished USF Health Professor and State of Florida World Class Scholar
Global Health Infectious Disease Research Program Department of Global Health University of South Florida
3720 Spectrum Blvd., Suite 304 Tampa, FL 33612
Home: (813) 345-8336 Work: (813) 974-0507/7807 Fax: (813) 974-0992
E-mail: [email protected] Education 1976- Massachusetts Institute of Technology: PhD under the direction of 1981 Dr. Gene M. Brown. Thesis entitled “The Purification and Properties of Dihydropterin Oxidase from Drosophila melanogaster.” 1972- Rutgers University: A.B. with highest honors 1976 Major in microbiology. Thesis entitled “The Effect of Cholera Enterotoxin on the Adenyl Cyclase of Escherichia coli.”
Employment 2012 - Distinguished USF Health University Professor, State of Florida present World Class Scholar and Chair, Department of Global Health, University of South Florida College of Public Health 2008- University of South Florida: Professor and State of Florida World 2012 Class Scholar, Global Health Infectious Disease Research, Department of
Global Health. Joint Appointment in Molecular Medicine 1997- University of Alabama, Birmingham: Professor, Division 2007 of Geographic Medicine, Department of Medicine, with joint appointments in the Departments of Microbiology and Epidemiology and International Health.
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1989 - University of Alabama, Birmingham: Associate Professor, Division 1997 of Geographic Medicine, Department of Medicine 1985- Case Western Reserve University: Assistant Professor, Division 1989 of Geographic Medicine, Department of Medicine 1982- Harvard School of Public Health: Postdoctoral fellow in the 1985 Department of Tropical Public Health (laboratory of Dr. Dyann F. Wirth)
Peer Review Panels and Editorial Boards 1986- Temporary member, Scientific Working Group, Onchocerciasis 1993 Chemotherapy Project, World Health Organization 1988- Editorial Board, Experimental Parasitology 2002 1990- Ad Hoc Reviewer, Filariasis Scientific Working Group, World Present Health Organization 1990- Ad Hoc Member, Bacteriology and Mycology Study Section, NIAID 1992 1993 Member, Review Committee, “Basic and Applied Research into Tropical Diseases”, NIAID 1993 Member, Source Selection Committee, “Basic and Applied Research into Tropical Diseases”, NIAID 1993- Member, TMP/AHR Study Section 2002 1995 Chairman, Site Visit Committee AID-NIAID Middle Eastern 1996 Research
Cooperative (MERC) Program 2003- Editorial Board, Molecular and Biochemical Parasitology present
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2004- Chair, Assay and Methods Development Study Section, National 2006 Institutes of Health 2005- Scientific Advisory Board, WIRM Initiative, Scripps Institute, La Jolla, 2006 CA 2006 Ad Hoc reviewer, Pathogenic Eucaryotes Study Section, National Institutes of Health 2006 Reviewer, Pathways Program, National Institutes of Health 2006 Member, Drug Design Research Steering Committee, Special
Programme for Research and Training in Tropical Diseases, World Health Organization
2007 External Reviewer, SBIR Program, United States Department of Agriculture 2007- Editorial Board Advisor, PloS Neglected Tropical Diseases present 2007- Member, Ecology of Infectious Diseases Proposal Review Panel, 2008 National Science Foundation 2009 Member “Topics in Microbiology” Study Section, NIAID 2009- Editorial Board, Journal of Tropical Medicine 2012 2010- Editor in Chief, Research Reports in Tropical Medicine present 2010- Member Working Group on Eukaryotic Pathogens and Disease 2012 Vectors Target Selection, pathogens genomic sequencing initiative,
National Institutes of Health 2014 Chair, NIH BSTF55 Indo-US Collaboration Grant Study Section 2015 Chair, NIH PAR14-092 Bioengineering Research Partnership Study Section Manuscript Reviewer for Acta Tropica American Journal of Tropical Medicine and Hygiene Annals of Epidemiology Biochimica et Biophysica Acta
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European Journal of Biochemistry Experimental Parasitology FASEB Journal Filarial Journal Genomics Infection and Immunity Insect Molecular Biology International Journal for Parasitology Journal of Biological Chemistry Journal of Medical Entomology Journal of Immunology Journal of Infectious Diseases Journal of Parasitology The Lancet Microbial Reviews Memórias do Instituto Oswaldo Cruz Molecular and Biochemical Parasitology Molecular Phylogenetics and Evolution Nature Protocols Parasitology Parasitology Today PLOS ONE PLOS Neglected Tropical Diseases Transactions of the Royal Society of Tropical Medicine and Hygiene Trends in Parasitology Tropical Medicine and International Health Invited Presentations 1986 Invited speaker, University of Edinburgh, Edinburgh, Scotland 1986 Invited speaker, Rockefeller Great Neglected Diseases Program Meeting, Woods Hole Massachusetts 1987 Invited speaker, McArthur Foundation Symposium on Parasitic Diseases, Pleasant Town, New York 1988 Invited speaker, Texas College of Osteopathic Medicine, Fort Worth Texas 1988 Invited speaker, Division of Infectious Diseases, University of Alabama at Birmingham, Birmingham, Alabama 1989 Invited speaker, O Now! Symposium on Onchocerciasis, University of Leiden, Leiden, the Netherlands 1989 Invited speaker, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany 1989 Invited Speaker, University of Heidelberg, Heidelberg, Germany 1989 Invited participant, WHO workshop “ DNA probes for Filariasis and Onchocerciasis”, Jakarta, Indonesia 1989 Invited speaker, 24th Joint Conference on Parasitic Diseases, The
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US-Japan Cooperative Medical Science Program of the National Institutes of Health, Madison Wisconsin 1991 Invited speaker, Symposium: Nucleic Acid Based Diagnostics: Applications of the Polymerase Chain Reaction” 39th Annual Meeting of the American Society of Tropical Medicine and Hygiene, New Orleans, Louisiana 1991 External participant, Internal Technical Review Meeting of the Onchocerciasis Control Programme in West Africa, Ouagadougou, Burkina Faso 1991 Invited speaker, Agouron Institute, San Diego, California 1992 Invited participant, WHO workshop, “DNA probes for Diagnosis of Human and Animal Onchocerca” Bouake, Cote d’Ivoire 1992 External participant, Internal Technical Review Meeting of the Onchocerciasis Control Programme in West Africa, Ouagadougou, Burkina Faso 1992 Lecturer, MBL Summer Course “Biology of Parasitism” Woods Hole, Massachusetts 1993 Invited speaker, 2nd annual ICTDR meeting of the National Institutes of Health, Bethesda, Maryland 1993 Invited participant, WHO workshop “DNA Probes and PCR for Detection of Filarial Parasites in Vectors” Beverly, Massachusetts 1993 Invited speaker, 28th Joint Conference on Parasitic Diseases, The US-Japan Cooperative Medical Science Program of the National Institutes of Health, Baltimore, Maryland 1993 Lecturer, MBL Summer Course “Biology of Parasitism” Woods Hole, Massachusetts 1994 Invited Speaker and Rapporteur, WHO workshop “The Filarial Genome Project” Beverly Massachusetts 1994 Invited speaker, “Immunogenetics and Epidemiology of Aveolar Echinococcosis”, Schloss Reisenburg, Gunzburg, Germany 1994 Invited speaker, Fourth International Conference on Onchocerciasis, Pan American Health Organization, Washington, D.C. 1995 Invited Speaker, Institut Pierre Richet, OSTROM, Bouake Côte d’Iviore 1995 Invited speaker, 30th Joint Conference on Parasitic Diseases, The US-Japan Cooperative Medical Science Program of the National Institutes of Health, Cleveland, OH. 1995 Invited Speaker, Centers for Disease Control, Atlanta, GA. 1996 Invited Speaker, International Workshop on Molecular Epidemiology and Evolutionary Genetics of Pathogenic Microorganisms, Centers for Disease Control and Prevention and ORSTOM, Atlanta, GA. 1997 Invited speaker and rapporteur, O. volvulus Diagnostic methods workshop, World Health Organization, Geneva 1998 Keynote speaker, American Society of Tropical Medicine and Hygiene, 59th Annual Meeting
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1998 Invited speaker, Workshop on macrofilaricidal targets, World Health Organization, Geneva 2000 Noguchi Memorial Institute for Medical Research, Legon, Ghana 2000 Invited speaker, Black Flies in the New Millennium, Brock University, St. Catherine’s, Ontario, Canada 2002 Invited speaker, University of Georgia, Athens, GA. 2002 Invited speaker, McGill University, Montreal Canada 2003 Invited Speaker, Filarial Research Summit, Baltimore Md. 2004 Invited participant, Brugia malayi genome annotation jamboree, The Institute for Genome Research, Rockville, MD. 2004 Invited Speaker, University of South Alabama, Mobile, AL 2004 Participant, Global 2000 Onchocerciasis Annual Review, Carter Center, Atlanta, GA. 2004 Presenter, IACO meeting, Carter Center, Atlanta, GA 2005 Invited speaker, Annual Meeting of the American Thoracic Society, San
Diego, CA 2005 Invited speaker, Centro De Biotecnología Genómica, Instituto Politécnico
Nacional, Reynosa, Mexico 2005 Participant, Global 2000 Onchocerciasis Annual Review, Carter Center, Atlanta, GA. 2006 Symposium speaker, 8th National Conference on West Nile virus,
American Society of Microbiology and Centers for Disease Control and Prevention, San Francisco, CA.
2006 Participant and Presenter, 10th International Congress on Onchocerciasis in the Americas, Antigua, Guatemala
2006 Participant, Eukaryotic Pathogens and Vectors Sequencing Workshop, National Human Genome Research Institute
2006 Seminar Speaker, University of Pennsylvania 2006 Participant, Global 2000 Onchocerciasis Annual Review, Carter Center, Atlanta, GA. 2007 Participant, Global 2000 Onchocerciasis Annual Review, Carter Center, Atlanta, GA. 2007 Participant and Presenter, 11th International Congress on Onchocerciasis
in the Americas, Quito, Ecuador 2008 Seminar Speaker, New England Biolabs, Ipswitch, MA 2008 Scientific Advisory Board, WHO Multi-Disease Research Centre,
Ouagadougou, Burkina Faso 2008 Participant, Global 2000 Onchocerciasis Annual Review, Carter Center, Atlanta, GA. 2008 Participant and Presenter, 12th International Congress on Onchocerciasis
in the Americas, Antigua, Guatemala 2009 Participant, Global 2000 Onchocerciasis Annual Review, Carter Center, Atlanta, GA. 2009 Seminar Speaker, University of Georgia 2009 Participant and Presenter, 13th International Congress on Onchocerciasis
in the Americas, Oaxaca, Mexico
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2010 Participant and Presenter, 14th International Congress on Onchocerciasis in the Americas, Quito, Ecuador
2011 Participant, Global 2000 Onchocerciasis Annual Review, Carter Center, Atlanta, GA. 2011 Participant and Presenter, 15th International Congress on Onchocerciasis
in the Americas, Bogota, Colombia 2012 Participant, Global 2000 Onchocerciasis Annual Review, Carter Center, Atlanta, GA. 2012 Seminar Speaker, Case Western Reserve University 2016 Panelist “Zika at our doorstep” Roundtable University of South Florida 2016 Panelist, “Partners in Zika Prevention” Expert Roundtable University
of St. Petersburg Educational Activities Didactic Teaching: Prior to USF: 1978 & Teaching assistant, Biochemistry (course 7.05), 1980 Massachusetts Institute of Technology 1981 Director, Biology teaching laboratory (course 7.011) Massachusetts Institute of Technology 1990 & Course Director, Biology of Parasitism, Department of 1992 Microbiology, University of Alabama at Birmingham 1994 Lecturer, Biology of Parasitism, Woods Hole, MA 1995- Lecturer, Tropical Infectious Diseases, Department of 2007 Epidemiology and Public Health, University of Alabama at Birmingham (Arboviruses and onchocerciasis) 2003- Lecturer, Medical Microbiology, University of Alabama 2007 at Birmingham School of Medicine (Emerging viruses and helminthic Infections). 1995- US mentor, NIH Minority International Research Training present Program, University of Alabama at Birmingham 2003- Member, Department of Medicine Education Committee 2007 University of Alabama at Birmingham 2006- Principal Investigator, Bioterrorism and Emerging 2007 Infectious Diseases Training Grant, University of
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Alabama at Birmingham At USF: Course Director: 2009 PHC 6907 Independent Study PHC 6945 Supervised Field Experience PHC 6971 Thesis
PHC 7910 Directed Research
2010 PHC 6907 Independent Study PHC 6934-901 Laboratory Techniques in Public Health PHC 6971 Thesis
PHC 7908 Specialized Study PHC 7910 Directed Research
2011 PHC 6934-901 Laboratory Techniques in Public Health PHC 6971 Thesis
PHC 6977 Special Project PHC 7910 Directed Research 2012 PHC 6934 Tools of Research: Laboratory Rotations PHC 6977 Special Project PHC 7910 Directed Research PHC 7931-001 Advanced Interdisciplinary Seminar: Current topics in Global Health Infectious Disease Research PHC 7980 Dissertation 2013 PHC 6934 Tools of Research: Laboratory Rotations PHC 6977 Special Project PHC 7910 Directed Research PHC 7931-001 Advanced Interdisciplinary Seminar: Current topics in Global Health Infectious Disease Research Guest lecturer for: 2009: PHC 6512 Vectors of human Disease 2010: PHC 6512 Vectors of human Disease
PHC 6510 Emerging and Infectious Diseases BMS 6041 Medical Sciences 5
2011: PHC 6512 Vectors of human Disease
PHC 6510 Emerging and Infectious Diseases GMS 6103 Foundations in Medical Microbiology and Immunology
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BMS 6041 Medical Sciences 5 2012 GMS 6103 Foundations in Medical Microbiology and Immunology
BMS 6041 Medical Sciences 5 PhD level and post-graduate laboratory based training Pre-Doctoral/Postdoctoral Trainees in Thomas R. Unnasch Laboratory 1. Klaus Erttmann, Postdoctoral fellow, 1986-1989 Strain and Species Specific DNA probes for O. volvulus Present position: Bernhardt Nocht Institute, Hamburg, Germany 2. Michael Kron, Postdoctoral fellow, 1987-1989 Analysis of structure of an aspartyl tRNA synthetase gene from O. volvulus Present position: Professor, Medical College of Wisconsin 3. Peter Zimmerman, Graduate Student 1989-2003 (Ph.D granted 2003) Evolution of a repeated sequence family in O. volvulus Present position: Professor, Case Western University 4. James Tang, PhD, Postdoctoral fellow, 1993-1996 Vector-parasite transmission complexes in human onchocerciasis Present Position: Professor, Division of Infectious Diseases, University of Alabama at Birmingham 5. Elise Keddie, Graduate Student (Ph.D granted 1997) Mitochondrial structure in Onchocerca volvulus Present Position: Manager, Zero Emission Vehicle Implementation, California Air Resources Board 6. Nancy Maher, Graduate Student (Ph.D granted 2000) Molecular Epidemiology of Pneumocystis carinii Present position: Program Manager, VA non-profit office, Veterans Health Administration 7. Daniel Boakye, Postdoctoral Fellow, 1996-1999 Microsatellites to detect hybridization between O. volvulus strains Present position: Head, Entomology Unit, African Programme for Onchocerciasis Control 8. Joon Hak Lee, Ph.D Postdoctoral fellow, 1998-2000 Ecology of Encephalomyelitis Virus Infection in the Southeastern USA Present Position: Staff Scientist, Sullivan County Department of Health, New York
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9. Tarig Higazi, Ph.D, Postdoctoral fellow, 1998-2006 Promoter Analysis of B. malayi Present Position: Associate Professor, Ohio University, Zanesville 10. Limin Shu, Ph.D, Postdoctoral fellow, 2001-2004 Tansfection and Immortalization of filarial nematodes Present Position: Research Associate Professor, Rutgers University 11. Sunny Yung, BS, MD-Ph.D Student (Ph.D granted 2002) Plastid targeting in Toxoplasma gondii Present position: Research fellow, National Institutes of Health 12. Tonya Korves, BS, MS MPH student 2003-2005 (MPH granted 2005) Ecology of Eastern encephalomyelitis Virus in the Southeastern USA Present position: Professor, St. Louis Community College at Wildwood 13. Gregory White, Graduate Student 2006-2011(Ph.D granted 2011) Ecology of Eastern Equine Encephalitis Virus in the Southeastern USA Present position: Scientific Director, Cochilla Valley Mosquito Control District. 14. Christy Ottendorfer, Ph.D postdoctoral fellow 2008-2010 Ecology of Encephalitis Viruses in Florida Present Position: Microbiologist, Centers for Disease Control and Prevention, Atlanta, GA 15. Chitra Chauhan, Ph.D postdoctoral fellow, 2008-2010 (deceased) Transfection and analysis of transcription in Brugia malayi 16. Shulin Xu, DVM, postdoctoral fellow 2008-2011 Mapping Protein Interactions between Filaria and its Wolbachia Endosymbiont Present position: Post-doctoral fellow, University of South Florida 17. Canhui Liu, Ph.D postdoctoral fellow 2006-present Transfection and analysis of transcription in Brugia malayi 18. Nathan Burkett-Cadena, Ph.D Postdoctoral Fellow, 2010-2013 Development of an improved black trap for onchocerciasis monitoring and control. Present position: Assistant Professor, Department of Entomology, University of Florida 19. Patrick Vander Kelen, Ph.D Student 2009-2012 Spatial modeling of Eastern Equine Encephalitis Virus foci in Florida. Present Position: Staff Scientist, Oregon Department of Health 20. Andrea Bingham, Ph.D Student, 2010-2014
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Mechanisms of over-wintering and early season amplification of Eastern Equine Encephalitis Virus. Present position: Vector-Borne Disease Surveillance Coordinator, Florida Department of Health 21. Amruta Mashlikar, PhD Student 2012-2016 Ecdysteroid signaling in human filarial parasites. Present Position: Postdoctoral Fellow, New England Biolabs Masters of Science in Public Health Students in Thomas R. Unnasch Laboratory 1. Jessie Dyer 2008-2010. MSPH granted 2010. Thesis entitled “Characterization of unknown viruses from Florida” 2. Michelle Bailey 2009-2010. MSPH granted 2010. Thesis entitled “Core Promoter Function in B. malayi” 3. Andrea Bingham 2009-2011. MSPH granted 2011. Thesis entitled “Development of an ELISA for Eastern Equine Encephalitis Virus that can Differentiate Infected from Vaccinated Horses” 4. Tracy Enright 2009-2011. MSPH granted 2011. Thesis entitled “Analysis of Ecdysone response elements in the genome of Brugia malayi” 5. Alex Talsma 2011-2013 MSPH granted 2013. Thesis entitled “Development of a confirmatory assay to detect Onchocerca volvulus larvae in pools of blackflies” 6. Shanna Boclen 2012-2014 MSPH granted 2014. Thesis entitled “Molecular Evidence for Vector Implication of Onchocerca lupi in Los Angeles County, CA” 7. Monica Lanzaro 2013-2015 MSPH granted 2015.Thesis entitled “Gene Expression Analysis Of Upregulated Genes By 20-OH Ecdysone in Brugia malayi Short-term trainees trained in USA from the following countries: Brazil Burkina Faso Cameroon Canada Colombia Cote d’Ivoire Ecuador Ethiopia Guatemala Ghana Mexico
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Nigeria Venezuela Sudan Uganda Supervision of Overseas Research Studies by USA Students in: Burkina Faso Cote d’Ivoire Ghana Guatemala Uganda Administrative Experience: 2012 – present Chair, Department of Global Health, USF 2009 - 2012 Co-Director, Center for Biological Defense, USF 2008 – present Director and Principal Investigator, BSL3 facility, IDRB
University Service: 2011 – 2012 COPH Research Advisory Committee 2012 COPH Appointment, promotion and tenure (APT) committee 2010 – 2012 Global Health APT committee 2012 Chair, Global Health APT committee 2012 Chair, Global Health PhD application review committee
External/International Service: 1993- Member, Program Committee, American Society of Tropical 1996 Medicine and Hygiene 1987- Member, Young Investigator Award Committee, American Society of 1993 Tropical Medicine and Hygiene 1992- Temporary Technical Advisor, Onchocerciasis Control Programme in 2000 West Africa, Ouagadougou, Burkina Faso 2001- Technical Advisor, Onchocerciasis Elimination Program in the 2014 Americas, Guatemala City, Guatemala 2001- Technical Advisor, Molecular Monitoring, River Blindness Program, present The Carter Center, Atlanta, GA 2008- Technical Advisor, African Programme for Onchocerciasis Control, 2014 Ouagadougou, Burkina Faso
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2008- Member, Ugandan Onchocerciasis Elimination Committee, Ugandan 2011 Ministry of Health 2011 Chair, Ugandan Onchocerciasis Elimination Expert Advisory present Committee, Uganda Ministry of Health 2011 Member, Board of Directors, Florida Mosquito Control Association 2015 2012 - Member, Mectizan Expert Committee, Mecitzan Donation Program, present Atlanta, GA 2012 - Member, Guidelines Development Group, World Health Organization 2015 Geneva 2013 - Member, Technical Coordinating Committee (TCC) African 2015 Programme for Onchocerciasis Control 2014- Chair, Transition Task Force, (TTF) African Programme for 2015 Onchocerciasis Control 2014- Member, Ethiopian Onchocerciasis Elimination Expert Advisory present Committee, Ethiopian Ministry of Health Honors and Awards: 1975 Henry Rutgers Scholar 1976 Recipient, Theobold Smith Society Award 1982 Recipient, individual NRSA fellowship 1982 Alternate, Helen Hay Whitney Fellowship 2007 State of Florida World Class Scholar 2010 Outstanding Research Achievement Award, University of South Florida 2012 Distinguished USF Health Professor, University of South Florida 2014 Fellow, American Society of Tropical Medicine and Hygiene
Funding History Past Grant Support: 1. WHO # 860275 “Development of a DNA Based Assay to Distinguish Forest and Savannah Strains of O. volvulus. 1/1/87-6/30/90 $110,036.
2. WHO # 870068 “Characterization of a Gene for a Potentially Protective Antigen of Onchocerca volvulus.” 7/1/87-6/30/89 $73,750.
3. Edna McConnell Clark Foundation # 05189 “Characterization and Molecular Cloning
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of Potentially Protective Antigens of O. volvulus” (Listed as Co-P.I.) 3/1/89-2/28/91 $198,000.
4. WHO # 890307 “Refinement of a DNA Probe Based Assay to Distinguish Forest and Savannah Forms of Onchocerca volvulus.” 7/1/90-12/31/92 $110,013.
5. Edna McConnell Clark Foundation “Potentially Protective Peptide Antigens of O. volvulus” 4/1/90-9/30/93 $390,000.
6. NIH Program Project “Molecular Parasitology and Parasitic Disease.” David O. Freedman, Program Director. P.I. on Sub-Project titled: “Molecular Cloning of Disease Associated Antigens” 3/1/91-12/31/95 $383,780.
7. NIH 1R29AI29693 “Identification of O. volvulus Protective Epitopes.” 8/1/89-7/31/95 $349,995.
8. WHO #920288 “Analysis of a Repeated Sequence Family to Study the Relationship Between Onchocerca volvulus Strains” 1/1/93-6/30/96 $71,196.
9. Edna McConnell Clark Foundation “Steroid Receptors as Vaccine Candidates Against Onchocerca volvulus. 4/1/93-3/31/96 $225,000.
10. NIH 1RO1AI33008 “Vector-Parasite Complexes in Onchocerciasis” (TR Unnasch, PI) 5/1/93-4/30/97 $327,539.
11. WHO #950453 “Strain Variation in Pathogenetic Autoantigen Genes in Onchocerca volvulus. (TR Unnasch, PI) 1/1/96-12/31/97 $57,574.
12. Edna McConnell Clark Foundation “Molting regulatory and biochemical activities as vaccine targets against Onchocerca volvulus. 4/1/96-12/31/97 (TR Unnasch, PI) $80,000
13. NIH R01 AI 35938 “Cytokine based therapy of Onchocerca antigen induced keratitis in the mouse” (E. Pearlman, PI, TR Unnasch, co-investigator) 4/1/94-3/31/98 $67,205 (TR Unnasch’s subproject)
14. WHO 960040 “Prolyl 4-hydroxylase as a Chemotherapeutic Target against Onchocerca volvulus” (TR Unnasch, PI) 8/1/96-7/31/98 $134,988
15. NIH 1 R21 AI39245-01 “Molecular Epidemiology of Onchocerciasis in Nigeria” 9/23/96-9/22/98 (TR Unnasch, PI) $116,302.
16. Edna McConnell Clark Foundation “Use of Pantropic retroviruses to develop an immortalized cell line from Onchocerca volvulus.” (TR Unnasch, PI) 01/01/98 - 12/31/99 $119,000
17. WHO #970476 “Microsatellite Loci to Detect Hybridization between Onchocerca volvulus strains” (TR Unnasch PI) 11/1/98 - 01/06/01 $38,541
18. WHO “Characterization of Angiogenic Proteins from Onchocerca volvulus.” (TR Unnasch PI) 01/01/00 = 12/31/01 $153,513
19. WHO #991105 “Survey of genetic polymorphisms in Loa loa associated with adverse reactions in ivermectin.” (TR Unnasch PI) 01/01/01 - 12/31/01 $ 12,338
20. Onchocerciasis Control Programme “Research of DNA Probes for Onchocerca spp.
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and Validation of Species Identification Performed by the OCP. (TR Unnasch PI) 1/1/93-12/31/02 $235,000.
21. NIAID 1 R01 AI54586 “Environmental and Molecular Epidemiology of P. carinii” (TR Unnasch Co-PI) 8/15/98 - 4/30/03 $165,530
22. Case Western Reserve University\WHO “Role of chemokines in the Pathogenesis of Onchocercal Dermatitis” (TR Unnasch PI) 01/01/99 - 12/31/03 $8,505
23. Tennessee Valley Authority #98RE4-233022 “Pool screen PCR for detecting encephylamyelitis viruses in mosquitoes in the Tennessee River Valley Authority area.” 1/01/00 - 12/01/04 $325,000
24. Case Western Reserve University\WHO “Role of chemokines in the Pathogenesis of Onchocercal Dermatitis” (E. Pearlman, PI) 01/01/05 - 12/31/05 $7,500
25. NIAID 1R01TW/ES01560 Ecology of Encephalitis virus in the Southeastern USA 10/1/00-6/30/05 $1,350,868
26. NIAID/NIH 5 T32 AI055438 Agents of International Health and Bioterrorism Concern 09/01/2003-12/31/2007 $1,234,050
27. NIAID 1R01CI00226 Ecology of West Nile Virus in the Southeastern USA 9/27/04-3/31/08 $525,000
28. NIAID 2R01AI48562 “Transfection and Analysis of Transcription in B. malayi” 1/1/01-1/31/10 $1,825,000
29. Department of Defense (FBRC through USF CBD) “Development of Rapid Methods for Improving PH Lab Detection of Emerging Arboviruses” 8/4/10 – 8/31/12 $ 107,480.
30. Carter Center/Global 2000 Program “PCR pool screening to monitor progress towards onchocerciasis elimination” 1/1/01-8/31/12 $242,000
31. NIH/NIAID 2 R01 AI049724-05 “Ecology of Encephalitis Viruses in the USA” 3/1/06-2/28/13 $2,263,765
32. NIH/NIAID R01AI072465-01A1”Mapping Protein Interactions between Filaria and its Wolbachia Endosymbiont” 5/1/08 - 4/30/13 $1,608,389.
33. Department of Defense (FBRC through USF CBD) “Ligands Expressing Bacteriophage as Anti-Viral Agents” 8/17/11 – 7/24/13 $76,468.
34. NIH/NIAID 1R56AI101072-01 “Delineating EEEV Over-Wintering and Early Season Amplification Mechanisms” 08/01/13 – 07/31/15 $343,255
Active Grant Support:
1. NIH Fogerty International Center #R01TW008508 “Spatial modeling of onchocerciasis foci in Africa by remote sensing” 08/15/09 – 11/30/15 $1,572,200.
2. Bill & Melinda Gates Foundation #OPP1017870 “Development of an improved black
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fly trap for onchocerciasis monitoring and control” 09/30/10 – 12/31/16 $2,142,862
3. NIH/NIAID R56AI01372-01A1 “Molecular Mechanisms of Filarial Endosymbiosis” (co-PI) $124,374
4. NIH/NIAID 1R21AI103302-01 “Ecdysteroid Signaling in Filarial Parasites” 08/15/13 – 07/31/16 $217,647
5. The Carter Center, Inc. “Onchocerca volvulus Parasite Detection” 09/01/13 – 08/31/16 $139,876
Publications Peer Reviewed Research Articles: 1. Bingham, A.M., N.D. Burkett-Cadena, H.K. Hassan, and T.R. Unnasch, Vector
Competence and Capacity of Culex erraticus (Diptera: Culicidae) for Eastern Equine Encephalitis Virus in the Southeastern USA. Journal of Medical Entomology, 2016. 53:473-476.
2. Botto, C., M.-G. Basañez, M. Escalona, N.J. Villamizar, O. Noya-Alarcón, J. Cortez, S. Vivas-Martínez, P. Coronel, H. Frontado, J. Flores, B. Graterol, O. Camacho, Y. Tovar, D. Borges, A.L. Morales, D. Ríos, F. Guerra, H. Margeli, M.A. Rodriguez, T.R. Unnasch, and M.E. Grillet, Evidence of suppression of onchocerciasis transmission in the Venezuelan Amazonian focus. Parasites & Vectors, 2016. 9:40. 10.1186/s13071-016-1313-z.
3. Burkett-Cadena, N.D., J. Gibson, M. Lauth, T. Stenn, C. Acevedo, R. Xue, J. McNelly, E. Northey, H.K. Hassan, A. Fulcher, A.M. Bingham, J. van Olphen, A. van Olphen, and T.R. Unnasch, Evaluation of the Honey-Card Technique for Detection of Transmission of Arboviruses in Florida and Comparison With Sentinel Chicken Seroconversion. Journal of Medical Entomology, 2016. in press. doi: 10.1093/jme/tjw106.
4. Gervasi, S.S., N.D. Burkett-Cadena, S.C. Burgan, A.W. Schrey, H.K. Hassan, T.R. Unnasch, and L.B. Martin, Host stress hormones alter vector feeding preferences, success and productivity. Proceedings of the Royal Society of London B: Biological Sciences, 2016. in press.
5. Golden, A., E.J. Stevens, L. Yokobe, D. Faulx, M. Kalnoky, R. Peck, M. Valdez, C. Steel, P. Karabou, M. Banla, P.T. Soboslay, K. Adade, A.H. Tekle, V.A. Cama, P.U. Fischer, T.B. Nutman, T.R. Unnasch, T. de los Santos, and G.J. Domingo, A Recombinant Positive Control for Serology Diagnostic Tests Supporting Elimination of Onchocerca volvulus. PLoS Neglected Tropical Diseases, 2016. 10:e0004292. doi: 10.1371/journal.pntd.0004292. eCollection 2016 Jan.
6. Jacob, B.G., R.J. Novak, L.D. Toe, M.S. Sanfo, K. Lassane, D.A. Griffin, T.L. Lakwo, P. Habomugisha, M. Katabarwa, and T.R. Unnasch, Ecogeographically and Non-Ecogeographically Prognosticating Discontinuously Canopied Simulium damnosum s.l. Habitats by Interpolating Metrizable Sub-Mixel Mean Solar Exoatmospheric Quantum Scalar Irradiance where θi is a Zenith Angle with Diatomically Etiolated Xanthophylls and Azimutually Isotropic Sources of Chloroplastic Carotenoid Zeaxanthins Geo-spectrotemporally Extracted from an Expositorily Uncoalesced Proxy RapidEye™ Red
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Edge Normalized Difference Vegetation Index Target Biosignature: A Case Study in Burkina Faso and Uganda. Journal of Remote Sensing and Geographic Information Systems, 2016. in press.
7. Katabarwa, M.N., J. Katamanywa, T. Lakwo, P. Habomugisha, E. Byamukama, D. Oguttu, C. Nahabwe, M. Ngabirano, E. Tukesiga, A. Khainza, E. Tukahebwa, T.R. Unnasch, F.O. Richards, and R. Garms, The Imaramagambo Onchocerciasis Focus in Southwestern Uganda: Interruption of Transmission After Disappearance of the Vector Simulium neavei and Its Associated Freshwater Crabs. American Journal of Tropical Medicine and Hygiene, 2016. in press. 10.4269/ajtmh.16-0181.
8. Mhashilkar, A.S., S.R. Adapa, R.H. Jiang, S.A. Williams, W. Zaky, B.E. Slatko, A.N. Luck, A.R. Moorhead, and T.R. Unnasch, Phenotypic and molecular analysis of the effect of 20-hydroxyecdysone on the human filarial parasite Brugia malayi. International Journal for Parasitology, 2016. 46:333-41. doi: 10.1016/j.ijpara.2016.01.005. Epub 2016 Feb 16. 10.1016/j.ijpara.2016.01.005. 26896576
9. Mhashilkar, A.S., S.L. Vankayala, C. Liu, F. Kearns, P. Mehrotra, G. Tzertzinis, S.R. Palli, H.L. Woodcock, and T.R. Unnasch, Identification of Ecdysone Hormone Receptor Agonists as a Therapeutic Approach for Treating Filarial Infections. PLoS Neglected Tropical Diseases, 2016. 10:e0004772. doi: 10.1371/journal.pntd.0004772. 27300294
10. Verocai, G.G., T.W. McGaha, J.P. Iburg, C.R. Katholi, E.W. Cupp, R. Noblet, and T.R. Unnasch, Identification of semiochemicals attractive to Simulium vittatum (IS-7). Medical and Veterinary Entomology, 2016. in press.
11. Voronin, D., S. Bachu, M. Shlossman, T.R. Unnasch, E. Ghedin, and S. Lustigman, Glucose and glycogen metabolism in Brugia malayi is associated with Wolbachia symbiont fitness. PLoS One, 2016. in press.
12. Zarroug, I.M., K. Hashim, W.A. ElMubark, Z.A. Shumo, K.A. Salih, N.A. ElNojomi, H.A. Awad, N. Aziz, M. Katabarwa, H.K. Hassan, T.R. Unnasch, C.D. Mackenzie, F. Richards, and T.B. Higazi, The First Confirmed Elimination of an Onchocerciasis Focus in Africa: Abu Hamed, Sudan. American Journal of Tropical Medicine and Hygiene, 2016. 27:16-0274.
13. Burkett-Cadena, N., E.M. Blosser, R.M. Young, L. Toe, and T.R. Unnasch, Carbon dioxide generated from carbonates and acids for sampling blood-feeding arthropods. Acta Tropica, 2015. 149:254-261.
14. Burkett-Cadena, N.D., A.M. Bingham, B. Hunt, G. Morse, and T.R. Unnasch, Ecology of Culiseta melanura and Other Mosquitoes (Diptera: Culicidae) from Walton County, Florida During Winter Period 2013-2014. Journal of Medical Entomology, 2015. 52:1074-82.
15. Downs, J., G. Hyzer, E. Marion, Z.J. Smith, P. Vander Kelen, and T.R. Unnasch, Mapping eastern equine encephalitis virus risk for white-tailed deer in Michigan. Applied Geography, 2015. 1:66-73.
16. Hassan, H.K., S. Bolcen, J. Kubofcik, T.B. Nutman, M.L. Eberhard, K. Middleton, J.W. Wekesa, G. Ruedas, K.J. Nelson, R. Dubielzig, M. De Lombaert, B. Silverman, J.J. Schorling, P.H. Adler, T.R. Unnasch, and E.S. Beeler, Isolation of Onchocerca lupi in dogs and black flies, California, USA. Emerging Infectious Diseases, 2015. 21:789-796.
17. Kelly-Hope, L.A., T.R. Unnasch, M.C. Stanton, and D.H. Molyneux, Hypo-endemic onchocerciasis hotspots: defining areas of high risk through micro-mapping and environmental delineation. Infect Dis Poverty, 2015. 4:36. 10.1186/s40249-015-0069-6.
18
18. Lakwo, T.L., R. Garms, E. Tukahebwa, A.W. Onapa, E. Tukesiga, J. Ngorok, J. Katamanywa, C. Twebaze, P. Habomugisha, D. Oguttu, E. Byamukama, M. Katabarwa, F. Richards, and T. Unnasch, Successful Interruption of the Transmission of Onchocerca volvulus in Mpamba-Nkusi Focus, Kibaale District, Mid-Western Uganda. East African Medical Journal, 2015. 92:401-407.
19. Liu, C., D. Voronin, C.B. Poole, S. Bachu, M.B. Rogers, J. Jin, E. Ghedin, S. Lustigman, L.A. McReynolds, and T.R. Unnasch, Functional Analysis of MicroRNA activity in Brugia malayi. International Journal for Parasitology, 2015. 45:579-583. doi: 10.1016/j.ijpara.2015.04.004. PMCID: PMC4490087
20. McGaha, T.W., R.M. Young, N. Burkett-Cadena, J.P. Iburg, J.M. Beau, S. Hassan, C.R. Katholi, E.W. Cupp, B.J. Baker, T.R. Unnasch, and R. Noblet, Identification Of Communal Oviposition Pheromones From The Black Fly Simulium vittatum. PLoS One, 2015. 10:e118904. 10.1371/journal.pone.0118904.
21. Richards, F., Jr., N. Rizzo, C.E. Diaz Espinoza, Z.M. Monroy, C.G. Crovella Valdez, R.M. de Cabrera, O. de Leon, G. Zea-Flores, M. Sauerbrey, A.L. Morales, D. Rios, T.R. Unnasch, H.K. Hassan, R. Klein, M. Eberhard, E. Cupp, and A. Dominguez, One Hundred Years After Its Discovery in Guatemala by Rodolfo Robles, Onchocerca volvulus Transmission Has Been Eliminated from the Central Endemic Zone. Am J Trop Med Hyg., 2015. 93:1295-304. 10.4269/ajtmh.15-0364. .
22. Rodriguez-Perez, M.A., N.A. Fernandez-Santos, M.E. Orozco-Algarra, J.A. Rodriguez-Atanacio, A. Dominguez-Vazquez, K.B. Rodriguez-Morales, O. Real-Najarro, F.G. Prado-Velasco, E.W. Cupp, F.O. Richards, Jr., H.K. Hassan, J.F. Gonzalez-Roldan, P.A. Kuri-Morales, and T.R. Unnasch, Elimination of Onchocerciasis from Mexico. PLoS Neglected Tropical Diseases, 2015. 9:e0003922. 10.1371/journal.pntd.0003922. 4498594
23. Stockwell, T., L. Heberlein-Larson, Y. Tan, R. Halpin, N. Fedorova, D. Katzel, S. Smole, T. Unnasch, L. Kramer, and S. Das, First complete genomic sequence of two Keystone Viruses from Florida, USA. Genome Announcements, 2015. 3:1-2.
24. Young, R.M., N.D. Burkett-Cadena, T.W. McGaha, M.A. Rodriguez-Perez, L.D. Toé, M.A. Adeleke, M. Sanfo, T. Soungalo, C.R. Katholi, R. Noblet, H. Fadamiro, J.L. Torres-Estrada, M.C. Salinas-Carmona, B. Baker, T.R. Unnasch, and E.W. Cupp, Identification of Human Semiochemicals Attractive to the Major Vectors of Onchocerciasis. PLoS Neglected Tropical Diseases, 2015. 9:e3450. 10.1371/journal.pntd0003450.
25. Bingham, A., N. Burkett-Cadena, H.K. Hassan, C.J.W. McClure, and T.R. Unnasch, Field investigations of winter transmission of Eastern Equine Encephalitis virus in Florida. American Journal of Tropical Medicine and Hygiene, 2014. 91:685-693.
26. Burkett-Cadena, N., A. Bingham, C. Porterfield, and T.R. Unnasch, Innate preference or opportunism: Mosquitoes feeding on birds of prey at the Southeastern Raptor Center. Journal of Vector Ecology, 2014. 39:21-31.
27. Burkett-Cadena, N. and T.R. Unnasch, Conspiring invasive species? The case of Tiger Mosquitoes and Apple Snails. Wingbeats, 2014. Spring:4-9.
28. Burkett-Cadena, N.D., A.M. Bingham, and T.R. Unnasch, Sex-based avian host use by arbovirus vectors. Royal Society Open Science, 2014. 1:140262.
29. Jacob, B., R.J. Novak , L. Toe, M.S. Sanfo, S. Caliskhan, A. Pare, M. Noma, L. Yameogo, and T. Unnasch, Unbiasing a Stochastic Endmember Interpolator Using ENVI Object-Based Classifiers and Boolean Statistics for Forecasting Canopied Simulium
19
damnosum s.L. Larval Habitats in Burkina Faso. Geophysics and Remote Sensing, 2014. 2:109. 10.4172/21690049.1000109.
30. Jacob, B.G., R.J. Novak, L. Toe, M.S. Sanfo, R. Tibgueria, A. Pare, M. Noma, D. Griffith, and T.R. Unnasch, Denoising a box-jenkins autoregressive-integrated moving average model employing automated bandwidth selection procedures and pre-whitened Euclidean-based quadratic surrogates in PROC ARIMA for optimizing asymptotic expansions and simulations of onchocerciasis endemic transmission zones in Burkina Faso. Journal of Public Health and Epidemiology, 2014. 6:347-389.
31. Katabarwa, M., T. Lakwo, P. Habomugisha, S. Agunyo, E. Byamukama, D. Oguttu, R. Ndyomugyenyi, E. Tukesiga, G.O. Ochieng, F. Abwaimo, A. Onapa, D.W. Lwamafa, F. Walsh, T.R. Unnasch, and F.O. Richards, Transmission of Onchocerca volvulus by Simulium neavei in Mount Elgon focus of Eastern Uganda has been interrupted. American Journal of Tropical Medicine and Hygiene, 2014. 90:1159-66. 10.4269/ajtmh.13-0501. 4047747
32. Lovato, R., A. Guevara, R. Guderian, R. Proano, T. Unnasch, H. Criollo, H.K. Hassan, and C.D. MacKenzie, Interruption of Infection Transmission in the Onchocerciasis Focus of Ecuador Leading to the Cessation of Ivermectin Distribution. PLoS Neglected Tropical Diseases, 2014. 8: e2821.
33. Lustigman, S., E. Melnikow, S.B. Anand, A. Contreras, V. Nandi, J. Liu, A. Bell, T.R. Unnasch, M.B. Rogers, and E. Ghedin, Potential involvement of Brugia malayi cysteine proteases in the maintenance of the endosymbiotic relationship with Wolbachia. International Journal for Parasitology: Drugs and Drug Resistance, 2014. 4:267-277.
34. Oguttu, D., E. Byamukama, C.R. Katholi, P. Habomugisha, C. Nahabwe, M. Ngabirano, H.K. Hassan, T. Lakwo, M. Katabarwa, F.O. Richards, and T.R. Unnasch, Serosurveillance to Monitor Onchocerciasis Elimination: The Ugandan Experience. American Journal of Tropical Medicine and Hygiene, 2014. 90:339-345. 24343885
35. Rodríguez-Pérez, M.A., M.A. Adeleke, I.C. Rodríguez-Luna, E.W. Cupp, and T.R. Unnasch, Evaluation of a community-based trapping program to collect Simulium ochraceum sensu lato for verification of onchocerciasis elimination. PLoS Neglected Tropical Diseases, 2014. 8:e3249.
36. Toé, L.D., L. Koala, N.D. Burkett-Cadena, B.M. Traoré, M. Sanfo, S.R. Kambiré, E.W. Cupp, S. Traoré, L. Yameogo, D. Boakye, M.A. Rodríguez-Pérez, and T.R. Unnasch, Optimization of the Esperanza Window Trap for the collection of the African onchocerciasis vector Simulium damnosum sensu lato. Acta Tropica, 2014. 137:39-43.
37. Vander Kelen, P., J.A. Downs, T. Unnasch, and L. Stark, A risk index model for predicting eastern equine encephalitis virus transmission to horses in Florida. Applied Geography, 2014. 48:79-86.
38. Estep, L.K., C.J. McClure, P. Vander Kelen, N.D. Burkett-Cadena, S. Sickerman, J. Hernandez, J. Jinright, B. Hunt, J. Lusk, V. Hoover, K. Armstrong, L.M. Stark, G.E. Hill, and T.R. Unnasch, Risk of exposure to eastern equine encephalomyelitis virus increases with the density of northern cardinals. PLoS One, 2013. 8:e57879. 10.1371/journal.pone.0057879. PMC3585233
39. Golden, A., C. Steel, L. Yokobe, E. Jackson, R. Barney, J. Kubofcik, R. Peck, T.R. Unnasch, T.B. Nutman, T. de Los Santos, and G.J. Domingo, Extended Result Reading Window in Lateral Flow Tests Detecting Exposure to Onchocerca volvulus: A New Technology to Improve Epidemiological Surveillance Tools. PLoS One, 2013. 8:e69231.
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10.1371/journal.pone.0069231. 3720650 40. Higazi, T.B. and T.R. Unnasch, Biolistic transformation of Brugia malayi. Methods in
Molecular Biology, 2013. 940:103-15. 10.1007/978-1-62703-110-3_9. 41. Higazi, T.B., I.M.A. Zarroug, H.A. Mohamed, W.A. ElMubark, T.C.M. Deran, N. Aziz,
M. Katabarwa, H.K. Hassan, T.R. Unnasch, C.D. Mackenzie, F. Richards, and K. Hashim, Interruption of Onchocerciasis Transmission in the Abu Hamed Focus, Sudan. American Journal of Tropical Medicine and Hygiene, 2013. 89:51-57. PMC3748488
42. Jacob, B.G., R.J. Novak , L. Toe, M. Sanfo, D.A. Griffith, T.L. Lakwo, P. Habomugisha, M.N. Katabarwa, and T.R. Unnasch, Validation of a remote sensing model to identify Simulium damnosum s.l. breeding sites in sub-Saharan Africa. PLoS Neglected Tropical Diseases, 2013. 7:e2342. PMC3723572
43. Jacob , B.G., R.J. Novak , L. Toe, M. Sanfo, L. Yameogo, S. Caliskan, R. Tingueria, A. Pare, and T.R. Unnasch, Definability of combinatorial functions and their linear recurrence relationships within a polylogarithmic triangularizable matrix employing surjective bilipschitz functions and other isomorphisms of metric spaces for forecasting seasonal endemic onchocerciasis transmission zones. Journal of Pure and Applied Statistics, 2013. 2:401-460.
44. Jacob , B.G., R.J. Novak , L. Toe, M.S. Sanfo, S. Caliskan, R. Tingueria, A. Pare, L. Yameogo, D. Griffith, and T.R. Unnasch, Finite-Difference Derivatives of a First-Order Integral Approximation Quantized with a Default Dual Quasi-Newton Optimizer and a Pseudo-Lipschitzian Property for Predictive Mapping Spatially Inhomogeneous Simulium damnosum s.l. Explanatory Covariates. Journal of Statistics: Advances in Theory and Applications, 2013. 10:1-136.
45. Katabarwa, M.N., T. Lakwo, P. Habomugisha, S. Agunyo, E. Byamukama, D. Oguttu, E. Tukesiga, D. Unoba, P. Dramuke, A. Onapa, E.M. Tukahebwa, D. Lwamafa, F. Walsh, and T.R. Unnasch, Transmission of Onchocerca volvulus Continues in Nyagak-Bondo Focus of Northwestern Uganda after 18 Years of a Single Dose of Annual Treatment with Ivermectin. American Journal of Tropical Medicine and Hygiene, 2013. 89:293-300. 10.4269/ajtmh.13-0037. 3741251
46. Lakwo, T.L., R. Garms, T. Rubaale, M. Katabarwa, F. Walsh, P. Habomugisha, D. Oguttu, T. Unnasch, H. Namanya, E. Tukesiga, J. Katamanywa, J. Bamuhiiga, E. Byamukama, S. Agunyo, and F. Richards, The disappearance of onchocerciasis from the Itwara focus, Western Uganda after elimination of the vector Simulium neavei and 19 years of annual ivermectin treatments. Acta Tropica, 2013. 126:218-221. 10.1016/j.actatropica.2013.02.016.
47. Melnikow, E., S. Xu, J. Liu, A.J. Bell, E. Ghedin, T.R. Unnasch, and S. Lustigman, A potential role for the interaction of Wolbachia surface proteins with the Brugia malayi glycolytic enzymes and cytoskeleton in maintenance of endosymbiosis. PLoS Neglected Tropical Diseases, 2013. 7:e2151. PMC3617236
48. Rodríguez-Pérez, M., A. Domínguez-Vázquez, T.R. Unnasch, H.K. Hassan, J.I. Arredondo-Jiménez, M.E. Orozco-Algarra, K.B. Rodríguez-Morales, I.C. Rodríguez-Luna, and F.G. Prado-Velasco, Interruption of transmission of Onchocerca volvulus in the Southern Chiapas focus, México. PLoS Neglected Tropical Diseases, 2013. 7:e2133. PMC3617236
49. Rodríguez-Pérez, M.A., M.A. Adeleke, N.D. Burkett-Cadena, J.A. Garza-Hernández, F. Reyes-Villanueva, E.W. Cupp, L. Toé, M.C. Salinas-Carmona, A.D. Rodríguez-Ramírez,
21
C.R. Katholi, and T.R. Unnasch, Development of a novel trap for the collection of black flies of the Simulium ochraceum complex. PLoS One, 2013. 8:e76814.
50. Barrera, R., A. Bingham, H.K. Hassan, M. Amador, A.J. Mackay, and T.R. Unnasch, Vertebrate Hosts of Aedes aegypti and Aedes mediovittatus (Diptera: Culicidae) in Rural Puerto Rico. Journal of Medical Entomology, 2012. 49:917-921.
51. Bingham, A., S.P. Graham, N. Burkett-Cadena, G.S. White, and T.R. Unnasch, Detection of Eastern Equine Encephalomyelitis virus RNA in North American snakes. American Journal of Tropical Medicine and Hygiene, 2012. 87:1140-1144.
52. Burkett-Cadena, N., H.K. Hassan, M.D. Eubanks, E.W. Cupp, and T.R. Unnasch, Winter severity predicts the timing of host shifts in the mosquito Culex erraticus. Biology Letters, 2012. 10.1098/rsbl.2012.0075. PMID: 22399787
53. Estep, L.K., C.J. McClure, N.D. Burkett-Cadena, H.K. Hassan, T.R. Unnasch, and G.E. Hill, Developing models for the forage ratios of Culiseta melanura and Culex erraticus based on characteristics of avian hosts. Journal of Medical Entomology, 2012. 49:378-387. PMID: 22493858
54. Gopal, H., H.K. Hassan, M.A. Rodríguez-Pérez, L.D. Toé, S. Lustigman, and T.R. Unnasch, Oligonucleotide based magnetic bead capture of Onchocerca volvulus DNA for PCR Pool Screening of Vector Black Flies. PLoS Neglected Tropical Diseases, 2012. 6:e1712.
55. Graham, S.P., T. Chapman, H.K. Hassan, G. White, C. Guyer, and T.R. Unnasch, Serosurveillance of eastern equine encephalitis virus in amphibians and reptiles from Alabama, U.S.A. American Journal of Tropical Medicine and Hygiene, 2012. 86:540-544. PMID: 22403333
56. Jacob, B.G., R.J. Novak, L. Toe, M.S. Sanfo, A. Afriyie, M.A. Ibrahim, D.A. Griffith, E. Mutabakaru, and T.R. Unnasch, Quasi-likelihood techniques in a logistic regression equation for identifying Simulium damnosum s.l. larval habitats intra-cluster covariates in Togo. Geospatial Information Science, 2012. 15:1-17.
57. Katabarwa, M., F. Walsh, P. Habomugisha, T. Lakwo, S. Agunyo, D. Oguttu, T.R. Unnasch, D. Unoba, E. Byamukama, E. Tukesiga, R. Ndyomugyenyi, and F.O. Richards, Transmission of onchocerciasis in Wadelai focus of Northwestern Uganda has been interrupted and the disease eliminated. J Parasitol Res, 2012. 2012:748540.
58. Liu, C., T. Enright, G. Tzertzinis, and T.R. Unnasch, Identification of genes containing ecdysone response elements in the genome of Brugia malayi. Molecular and Biochemical Parasitology, 2012. 186:38-43.
59. Vander Kelen, P.T., J.A. Downs, N. Burkett-Cadena, C.L. Ottendorfer, K.L. Hill, S. Sickerman, J. Hernandez, J. Jinright, B. Hunt, J. Lusk, V. Hoover, K. Armstrong, R.S. Unnasch, L.M. Stark, and T.R. Unnasch, Habitat associations of Eastern Equine Encephalitis Transmission in Walton County Florida. Journal of Medical Entomology, 2012. 49:746-756. PMID: 22679885
60. Vander Kelen, P.T., J.A. Downs, L.M. Stark, R.W. Loraamm, J.H. Anderson, and T.R. Unnasch, Spatial epidemiology of eastern equine encephalitis in Florida. International Journal of Health Geographics, 2012. 11:47. 10.1186/1476-072x-11-47. PMC3517371
61. Vaughan, J.A., J.O. Mehus, C.A. Brewer, D.K. Kvasager, S. Bauer, J.L. Vaughan, H.K. Hassan, T.R. Unnasch, and J.A. Bell, Theoretical potential of passerine filariasis to enhnce the enzootic transmission of West Nile Virus. Journal of Medical Entomology, 2012. 49:1430-1441.
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62. Bailey, M., C. Chauhan, C. Liu, and T.R. Unnasch, The Role of Polymorphisms in the Spliced Leader Addition Domain in Determining Promoter Activity in Brugia malayi. Molecular and Biochemical Parasitology, 2011. 176:37-41. PMID: 21111761.
63. Burkett-Cadena, N., G.S. White, M.D. Eubanks, and T.R. Unnasch, Winter Biology of Wetland Mosquitoes as a Focus of Eastern Equine Encephalomyelitis Virus Transmission in Alabama, USA. Journal of Medical Entomology, 2011. 48:967-973. PMID: 21936314
64. Burkett-Cadena, N.D., C.J. McClure, R.A. Ligon, S.P. Graham, C.G. Guyer, G.E. Hill, S.S. Ditchkoff, M.D. Eubanks, H.K. Hassan, and T.R. Unnasch, Host reproductive phenology drives seasonal patterns of host use in mosquitoes. PLoS One, 2011. 7:e17681. PMID: 21408172
65. Estep, L.K., C.J.W. McClure, N. Burkett-Cadena, Hassan, H.K., T.L. Hicks, T.R. Unnasch, and H. G.E, A multi-year study of mosquito feeding patterns on avian hosts in a southeastern focus of eastern equine encephalitis virus. American Journal of Tropical Medicine and Hygiene, 2011. 84:718-726.
66. Gray, K.M., N.D. Burkett-Cadena, M.D. Eubanks, and T.R. Unnasch, Crepuscular flight activity of Culex erraticus (Diptera: Culicidae). Journal of Medical Entomology, 2011. 48:167-72.
67. Higazi, T., I. Zarroug, M. Hanan, M. Wigdan, D. Tong, N. Aziz, A., M. Katabarwa, H.K. Hassan, T.R. Unnasch, C.D. Mackenzie, and F. Richards, PCR pool screening used to compare the prevalence of infective black flies in two different Northern Sudan onchocerciasis foci American Journal of Tropical Medicine and Hygiene, 2011. 84:753-756.
68. Liu, C., P. Vander Kelen, E. Ghedin, S. Lustigman, and T.R. Unnasch, Analysis of Transcriptional Regulation of Tetracycline Responsive Genes in Brugia malayi. Molecular and Biochemical Parasitology, 2011. 180:106-111. PMID: 21944995.
69. Melnikow, E., S. Xu, J. Liu, L. Li, Y. Oskov, E. Ghedin, T.R. Unnasch, and S. Lustigman, Interaction of a Wolbachia WSP-like protein with a nuclear-encoded protein of Brugia malayi. International Journal for Parasitology, 2011. 41:1053-1061. PMID: 21782817.
70. Oliveira, A., C.R. Katholi, N.D. Burkett-Cadena, H.K. Hassan, S. Kristensen, and T.R. Unnasch, Temporal Analysis of Feeding Patterns of Culex erraticus in Central Alabama. Vector Borne and Zoonotic Diseases, 2011. 11:413-421.
71. White, G., C. Ottendorfer, S. Graham, and T.R. Unnasch, Competency of Reptiles and Amphibians for Eastern Equine Encephalitis Virus. American Journal of Tropical Medicine and Hygiene, 2011. 85:421-425. PMID: 21896798
72. White, G.S., B.E. Pickett, E.J. Lefkowitz, C.L. Ottendorfer, L.M. Stark, and T.R. Unnasch, Phylogenetic Analysis of Eastern Equine Encephalitis Virus Isolates from Florida. American Journal of Tropical Medicine and Hygiene, 2011. 84:709-717.
73. Xu, S., C. Liu, G. Tzertzinis, E. Ghedin, C.C. Evans, R. Kaplan, and T.R. Unnasch, In vivo transfection of developmentally competent Brugia malayi infective larvae. International Journal for Parasitology, 2011. 41:355-362. PMID: 21118694.
74. Burkett-Cadena, N.D., R.A. Ligon, M. Liu, H.K. Hassan, G.E. Hill, M.D. Eubanks, and T.R. Unnasch, Vector-host interactions in avian nests: do mosquitoes prefer nestlings over adults? American Journal of Tropical Medicine and Hygiene, 2010. 83:395-399.
75. Estep, L., N.D. Burkett-Cadena, G.E. Hill, R.S. Unnasch, and T.R. Unnasch, Estimation of dispersal distances of Culex erraticus in a focus of Eastern equine encephalitis virus in
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the southeastern United States. Journal of Medical Entomology, 2010. 47:977-986. 76. Hill, G.E., L. Sieferman, M. Liu, H. Hassan, and T.R. Unnasch, The Effects of West Nile
Virus on the Reproductive Success and Overwinter Survival of Eastern Bluebirds in Alabama. Vector Borne and Zoonotic Diseases, 2010. 10:159-163. doi:10.1089/vbz.2008.0211 PMID: 19589058.
77. Jacob, B.G., N.D. Burkett-Cadena, J. Luvall, S. Parack, C.J.W. McClure, E. L., G.E. Hill, E.W. Cupp, R.J. Novak , and T.R. Unnasch, Developing GIS-Based Eastern Equine Encephalitis Vector-host Models in Tuskegee, Alabama International Journal of Health Geographics, 2010. 9:12.
78. Liu, C., C. Chauhan, and T.R. Unnasch, The role of local secondary structure in the function of the trans splicing motif of Brugia malayi. Molecular and Biochemical Parasitology, 2010. 169:115-119. PMID: 19852985.
79. Liu, C., A. Oliveira, C. Chauhan, E. Ghedin, and T.R. Unnasch, Functional Analysis of Putative Operons in Brugia malayi. International Journal for Parasitology, 2010. 40:63-71. PMID: 19631652.
80. Rodriguez-Perez, M.A., T.R. Unnasch, A. Dominguez-Vasquez, A.L. Morales-Castro, F. Richards, G.P. Pena-Flores, E. Orozco-Algarra, and G. Prado-Velasco, Lack of active Onchocerca volvulus transmission in the Northern Chiapas focus of Mexico. American Journal of Tropical Medicine and Hygiene, 2010. 83:15-20.
81. Rodriguez-Perez, M.A., T.R. Unnasch, A. Dominguez-Vazquez, A.L. Morales-Castro, G.P. Pena-Flores, M.E. Algarra-Orozco, J.I. Arredondo-Jimenez, F. Richards, M.A. Vasquez-Rodriguez, and V.G. Rendon, Interruption of Transmission of Onchocerca volvulus in the Oaxaca Focus, Mexico. American Journal of Tropical Medicine and Hygiene, 2010. 83:21-27.
82. Tzertzinis, G., A.L. Egana, S.R. Palli, M. Robinson-Rechavi, C.R. Gissendanner, C. Liu, T.R. Unnasch, and C.V. Maina, Molecular evidence for a functional ecdysone signaling system in Brugia malayi. PLoS Neglected Tropical Diseases, 2010. 4:e625. PMID: 20231890.
83. Ghedin, E., T. Hailemariam, J. DePasse, X. Zhang, Y. Oksov, T.R. Unnasch, and S. Lustigman, Brugia malayi gene expression in response to targeted elimination of Wolbachia endosymbiont. PLoS Neglected Tropical Diseases, 2009. 3:e525. PMID: 19806204.
84. Graham, S.P., H.K. Hassan, N.D. Burkett-Cadena, C. Guyer, and T.R. Unnasch, Nestedness of Ectoparasite-Vertebrate Host Networks. PLoS One, 2009. 4:e7873.
85. Ligon, R.A., N.D. Burkett-Cadena, M. Liu, G.E. Hill, H. Hassan, and T.R. Unnasch, Assessing Mosquito Feeding Patterns on Nestling and Brooding Adult Birds Using Microsatellite Markers. American Journal of Tropical Medicine and Hygiene, 2009. 81:534-537.
86. Liu, C., C. Chauhan, C.R. Katholi, and T.R. Unnasch, The splice leader addition domain represents an essential conserved motif for heterologous gene expression in B. malayi. Molecular and Biochemical Parasitology, 2009. 166:15-21. PMID: 19428668.
87. O'Conner, L., J.B. Gingrich, T. Unnasch, and H.K. Hassan, Gonotrophic age structure of mosquitoes in the Culex pipiens complex (Diptera: Culicidae) and possible influences on host meal selection. Journal of Parasitology and Vector Biology, 2009. 13:25-30.
88. Ottendorfer, C.L., J.H. Ambrose, G.S. White, T.R. Unnasch, and L.M. Stark, Isolation of genotype V St. Louis encephalitis virus in Florida. Emerging Infectious Diseases, 2009.
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15:604-6. PMID: 19331744. 89. Ulloa, A., H.H. Ferguson, J.D. Mendez-Sanchez, R. Danis-Lozano, M. Casas-Martinez,
J.G. Bond, J.C. Garcia-Zebadua, A. Orozco-Bonilla, J.A. Juarez-Ordaz, J.A. Farfan-Ale, J.E. Garcia-Rejon, E.P. Rosado-Paredes, E. Edwards, N. Komar, H.K. Hassan, T.R. Unnasch, and M.A. Rodriguez-Perez, West Nile Virus Activity in Mosquitoes and Domestic Animals in Chiapas, Mexico. Vector Borne and Zoonotic Diseases, 2009. 9:555-60.
90. Burkett-Cadena, N.D., M.D. Eubanks, and T.R. Unnasch, Preference of female mosquitoes for natural and artificial resting sites. Journal of the American Mosquito Control Association, 2008. 24:228-235.
91. Burkett-Cadena, N.D., S.P. Graham, H.K. Hassan, C. Guyer, M.D. Eubanks, C.R. Katholi, and T.R. Unnasch, Blood feeding patterns of potential arbovirus vectors of the genus Culex targeting ectothermic hosts. American Journal of Tropical Medicine and Hygiene, 2008. 79:809-15.
92. de Oliveira, A.D., C.R. Katholi, and T.R. Unnasch, Characterization of the promoter of the Brugia malayi 12kDa small subunit ribosomal protein (RPS12) gene. International Journal for Parasitology, 2008. 38:1111-1119. PMID: 18364245.
93. Gu, W., T.R. Unnasch, C.R. Katholi, R. Lampman, and R.J. Novak, Fundamental issues in mosquito surveillance for arboviral transmission. Transactions of The Royal Society of Tropical Medicine and Hygiene, 2008. 102:817-22.
94. Rodriguez-Perez, M.A., C. Lizarazo-Ortega, H.K. Hassan, A. Dominguez-Vasquez, J. Mendez-Galvan, P. Lugo-Moreno, M. Sauerbrey, F. Richards, Jr., and T.R. Unnasch, Evidence for suppression of Onchocerca volvulus transmission in the Oaxaca focus in Mexico. American Journal of Tropical Medicine and Hygiene, 2008. 78:147-52.
95. Rodriguez-Perez, M.A., M.A. Lutzow-Steiner, A. Segura-Cabrera, C. Lizarazo-Ortega, A. Dominguez-Vazquez, M. Sauerbrey, F. Richards, Jr., T.R. Unnasch, H.K. Hassan, and R. Hernandez-Hernandez, Rapid suppression of Onchocerca volvulus transmission in two communities of the Southern Chiapas focus, Mexico, achieved by quarterly treatments with Mectizan. American Journal of Tropical Medicine and Hygiene, 2008. 79:239-44.
96. Cupp, E.W., H.K. Hassan, X. Yue, W.K. Oldland, B.M. Lilley, and T.R. Unnasch, West Nile Virus Infection in Mosquitoes in the Mid-South USA, 2002-2005. Journal of Medical Entomology, 2007. 44:117-125.
97. de Oliveira, A., T.R. Unnasch, K. Crothers, S. Eiser, P. Zucchic, J. Moir, C.B. Beard, G.G. Lawrence, and L. Huang, Performance of a Molecular Viability Assay for the Diagnosis of Pneumocystis Pneumonia in HIV-Infected Patients. Diagnostic Microbiology and Infectious Disease, 2007. 57:169-176.
98. Dennett, J.A., A. Bala, T. Wuithiranyagool, Y. Randle, C.B. Sargent, H. Guzman, M. Siirin, H.K. Hassan, M. Reyna-Nava, T.R. Unnasch, R.B. Tesh, R.E. Parsons, and R. Bueno, Jr., Associations between two mosquito populations and West Nile virus in Harris County, Texas, 2003-06. Journal of the American Mosquito Control Association, 2007. 23:264-75.
99. Ghedin, E., S. Wang, D. Spiro, E. Caler, Q. Zhao, J. Crabtree, J.E. Allen, A.L. Delcher, D.B. Guiliano, D. Miranda-Saavedra, S.V. Angiuoli, T. Creasy, P. Amedeo, B. Haas, N.M. El-Sayed, J.R. Wortman, T. Feldblyum, L. Tallon, M. Schatz, M. Shumway, H. Koo, S.L. Salzberg, S. Schobel, M. Pertea, M. Pop, O. White, G.J. Barton, C.K. Carlow, M.J. Crawford, J. Daub, M.W. Dimmic, C.F. Estes, J.M. Foster, M. Ganatra, W.F.
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Gregory, N.M. Johnson, J. Jin, R. Komuniecki, I. Korf, S. Kumar, S. Laney, B.W. Li, W. Li, T.H. Lindblom, S. Lustigman, D. Ma, C.V. Maina, D.M. Martin, J.P. McCarter, L. McReynolds, M. Mitreva, T.B. Nutman, J. Parkinson, J.M. Peregrin-Alvarez, C. Poole, Q. Ren, L. Saunders, A.E. Sluder, K. Smith, M. Stanke, T.R. Unnasch, J. Ware, A.D. Wei, G. Weil, D.J. Williams, Y. Zhang, S.A. Williams, C. Fraser-Liggett, B. Slatko, M.L. Blaxter, and A.L. Scott, Draft genome of the filarial nematode parasite Brugia malayi. Science, 2007. 317:1756-60. PMID: 17885136.
100. Lindblade, K.A., B. Arana, G. Zea-Flores, N. Rizzo, C.H. Porter, A. Dominguez, N. Cruz-Ortiz, T.R. Unnasch, G.A. Punkosdy, J. Richards, M. Sauerbrey, J. Castro, E. Catu, O. Oliva, and F.O. Richards, Jr., Elimination of Onchocerca volvulus transmission in the Santa Rosa focus of Guatemala. American Journal of Tropical Medicine and Hygiene, 2007. 77:334-41.
101. Liu, C., A. de Oliveira, T.B. Higazi, E. Ghedin, J. DePasse, and T.R. Unnasch, Sequences necessary for trans-splicing in transiently transfected Brugia malayi. Molecular and Biochemical Parasitology, 2007. 156:62-73. PMID: 17727976.
102. Patrican, L.A., L.E. Hackett, J.E. Briggs, J.W. McGowan, T.R. Unnasch, and J.H. Lee, Host-feeding patterns of Culex mosquitoes in relation to trap habitat. Emerging Infectious Diseases, 2007. 13:1921-3. PMID: 18258048.
103. Savage, H.M., D. Aggarwal, C.S. Apperson, C.R. Katholi, E. Gordon, H.K. Hassan, M. Anderson, D. Charnetzky, L. McMillen, E.A. Unnasch, and T.R. Unnasch, Host choice and West Nile virus Infection rates in blood fed mosquitoes, including members of the Culex pipiens complex, from Memphis and Shelby County, Tennessee 2002-2003. Vector Borne and Zoonotic Diseases, 2007. 7:365-386.
104. Eng, J.K., W.J. Blackhall, M.Y. Osei-Atweneboana, C. Bourguinat, D. Galazzo, R.N. Beech, T.R. Unnasch, K. Awadzi, G.W. Lubega, and R.K. Prichard, Ivermectin selection on beta-tubulin: evidence in Onchocerca volvulus and Haemonchus contortus. Molecular and Biochemical Parasitology, 2006. 150:229-35.
105. Katholi, C.R. and T.R. Unnasch, Important experimental parameters for determining infection rates in arthropod vectors using pool screening approaches. American Journal of Tropical Medicine and Hygiene, 2006. 74:779-785.
106. Richards, S.L., L. Ponnusammy, T.R. Unnasch, H.K. Hassan, and C.A. Apperson, Host-feeding Patterns of Aedes albopictus (Skuse) (Diptera: Culicidae) in relation to the availability of human and domestic animals in suburban landscapes of central North Carolina with notes on blood meal hosts of sympatric mosquito species. Journal of Medical Entomology, 2006. 43:543-551.
107. Rodríguez-Pérez, M.A., C.R. Katholi, H.K. Hassan, and T.R. Unnasch, A large-scale entomologic assessment of Onchocerca volvulus transmission by pool-screen PCR in Mexico. American Journal of Tropical Medicine and Hygiene, 2006. 74:1026-1033.
108. Rodríguez-Pérez, M.A., C.A. Núñez-González, C. Lizarazo-Ortega, A. Sánchez-Varela, M.C. Wooten, and T.R. Unnasch, Analysis of genetic variation in the ribosomal DNA internal transcribed spacer and the NADH dehydrogenase subunit 4 mitochondrial genes of the onchocerciasis vector Simulium ochraceum sensu lato. Journal of Medical Entomology, 2006. 43:701-706.
109. Unnasch, R.S., T. Sprenger, C.R. Katholi, E.W. Cupp, G.E. Hill, and T.R. Unnasch, A Dynamic Transmission Model of Eastern Equine Encephalomyelitis Virus. Ecological Modelling, 2006. 192:425-440.
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110. Higazi, T.B., A. DeOliveira, C.R. Katholi, L. Shu, J. Barchue, M. Lisanby, and T.R. Unnasch, Identification of elements essential for transcription in Brugia malayi promoters. Journal of Molecular Biology, 2005. 353:1-13. PMID: 16154590.
111. Higazi, T.B., A. Filiano, C. Katholi, K.Y. Dadzie, J.H. Remme, and T.R. Unnasch, Wolbachia endosymbiont levels in severe and mild strains of Onchocerca volvulus. Molecular and Biochemical Parasitology, 2005. 141:109-112. PMID: 15811532.
112. Ramirez-Ramirez, A., G. Sanchez-Tejeda, J. Mendez-Galvan, T.R. Unnasch, and A. Monroy-Ostria, Molecular studies of Onchocerca volvulus isolates from Mexico. Infect Genet Evol, 2005. 22:22.
113. Adjami, A.G., T. L, Y. Bissan, S. Bugri, L. Yaméogo, M. Kone, C.R. Katholi, and T.R. Unnasch, The Current Status of Onchocerciasis in the Forest/Savanna Transition Zone of Côte d’ Ivoire. Parasitology, 2004. 128:407-414.
114. Apperson, C.S., H.K. Hassan, B.A. Harrison, S.E. Aspen, H.M. Savage, A. Farajollahi, W. Crans, T.J. Daniels, R.C. Falco, M. Benedict, M. Anderson, L. McMillen, and T.R. Unnasch, Host feeding patterns of probable vector mosquitoes of West Nile Virus in the eastern United States. Vector Borne and Zoonotic Diseases, 2004. 4:71-82.
115. Cupp, E.W., K.J. Tennessen, W.K. Oldland, H.K. Hassan, G.E. Hill, C.R. Katholi, and T.R. Unnasch, Mosquito and arbovirus activity during 1997-2002 in a wetland in northeastern Mississippi. Journal of Medical Entomology, 2004. 41:495-501.
116. Cupp, E.W., D. Zhang, X. Yue, M.S. Cupp, C. Guyer, T. Korves, and T.R. Unnasch, Identification of reptilian and amphibian bloodmeals from mosquitoes in an Eastern Equine Encephalomyelitis virus focus in central Alabama. American Journal of Tropical Medicine and Hygiene, 2004. 71:272-276.
117. Higazi, T.B., A.D. Klion, M. Boussinesq, and T.R. Unnasch, Genetic Heterogeneity in Loa loa parasites from Southern Cameroon: A Preliminary Study. Filaria J, 2004. 3:4. PMID: 15225348.
118. Higazi, T.B., L. Shu, and T.R. Unnasch, Development and transfection of short term primary cell cultures from Brugia malayi. Molecular and Biochemical Parasitology, 2004. 137:345-348. PMID: 15383305.
119. Higazi, T.B. and T.R. Unnasch, Intron encoded sequences necessary for trans splicing in transiently transfected Brugia malayi. Molecular and Biochemical Parasitology, 2004. 137:181-184. PMID: 15279965.
120. Rodríguez-Pérez, M.A., B.G. Lilley, A. Domínguez-Vázquez, R. Segura-Arenas, C. Lizarazo-Ortega, A. Mendoza-Herrera, F. Reyes-Villanueva, and T.R. Unnasch, Polymerase Chain Reaction monitoring of transmission of Onchocerca volvulus in two endemic states in Mexico. American Journal of Tropical Medicine and Hygiene, 2004. 70:38-45.
121. Cupp, E.W., K. Klingler, H.K. Hassan, L.M. Viguers, and T.R. Unnasch, Transmission of eastern equine encephalomyelitis virus in central Alabama. American Journal of Tropical Medicine and Hygiene, 2003. 68:495-500. 2575747
122. Davis, C.T., D.W.C. Beasley, H. Guzman, P. Raj, M. D’Anton, R.J. Novak, T.R. Unnasch, R.B. Tesh, and A.D.T. Barrett, Genetic variation among temporally and geographically distinct West Nile virus isolates collected in the United States during 2001 and 2002. Emerging Infectious Diseases, 2003. 9:1423-1429.
123. Guevara, A.G., J.C. Vieira, B.G. Lilley, A. López, N. Vieira, J. Rumbea, R. Collins, and T.R. Unnasch, Entomolological Evaluation by Pool Screen Polymerase Chain Reaction
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of Onchocerca volvulus Transmission in Ecuador Following Mass Mectizan Distribution. American Journal of Tropical Medicine and Hygiene, 2003. 68:222-227.
124. Hassan, H.K., E.W. Cupp, G.E. Hill, C.R. Katholi, K. Klingler, and T.R. Unnasch, Avian host preference by vectors of Eastern Equine Encephalomyelitis virus. American Journal of Tropical Medicine and Hygiene, 2003. 69:641-647.
125. Higazi, T.B., E. Pearlman, D.R. Whikehart, and T.R. Unnasch, Angiogenic activity of an Onchocerca volvulus Ancylostoma secreted protein homologue. Molecular and Biochemical Parasitology, 2003. 129:61-68. PMID: 12798507.
126. Huang, L., K. Crothers, A. DeOliveira, S. Eiser, P. Zucchi, C.B. Beard, and T.R. Unnasch, Application of an mRNA-based molecular viability assay to oropharyngeal washes for the diagnosis of Pneumocystis pneumonia in HIV-infected patients. A pilot study. J Eukaryot Microbiol., 2003. 50:618-20.
127. Shu, L., C. Katholi, T. Higazi, and T.R. Unnasch, Analysis of the Brugia malayi HSP70 promoter using a homologous transient transfection system. Molecular and Biochemical Parasitology, 2003. 128:67-75. PMID: 12706798.
128. Yung, S.C., T.R. Unnasch, and N. Lang-Unnasch, Cis and trans factors involved in apicoplast targeting of Toxoplasma gondii. Journal of Parasitology, 2003. 89:767-776. PMID: 14533689.
129. Apperson, C.S., B.A. Harrison, T.R. Unnasch, H.K. Hassan, W.S. Irby, H.M. Savage, S.E. Aspen, D.W. Watson, L.M. Rueda, B.R. Engber, and R.S. Nasci, Host-feeding habits of Culex and other mosquitoes (Diptera: Culicidae) in the borough of Queens in New York City, with characters and techniques for identification of Culex mosquitoes. Journal of Medical Entomology, 2002. 39:777-785.
130. Higazi, T.B., A. Merriweather, L. Shu, R. Davis, and T.R. Unnasch, Brugia malayi: Transient transfection by microinjection and particle bombardment. Experimental Parasitology, 2002. 100:95-102. PMID: 12054699.
131. Lee, J.H., H. Hassan, G. Hill, E.W. Cupp, T.B. Higazi, C.J. Mitchell, M.S. Godsey, and T.R. Unnasch, Identification of mosquito avian-derived blood meals by polymerase chain reaction-heteroduplex analysis. American Journal of Tropical Medicine and Hygiene, 2002. 66:599-604. PMID: 12201598.
132. Lee, J.H., K. Tennessen, B.H. Lilley, and T.R. Unnasch, Simultaneous Detection of Three Mosquito-borne Encephalitis Viruses (Eastern Equine, LaCrosse and St. Louis) Using a Single Tube Multiplex Reverse Transcription-PCR Assay. Journal of the American Control Association, 2002. 18:26-31. PMID: 11998926.
133. Maher, N., H.K. Dillon, A. Awooda, J.H. Lee, S.H. Vermund, and T.R. Unnasch, A Comparison of two Surface Sample Collection Devices for Use in Polymerase Chain Reaction Based Detection of Pneumocystis carinii in House Dust. Applied Occupational and Environmental Hygiene, 2002. 17:416-423. PMID: 12049431.
134. Oyibo, W.A., F.A. Fagbenro-Beyioku, A. Merriweather, and T.R. Unnasch, Molecular characterization of Onchocerca volvulus in a middlebelt state of Nigeria. African Journal of Science and Technology, 2002. 3:22-24.
135. Higazi, T.B., C.R. Katholi, B.M. Mahmoud, O.Z. Baraka, M.M. Mukhtar, Y. Al Qubati, and T.R. Unnasch, Onchocerca volvulus: Genetic Diversity of Parasite Isolates from Sudan. Experimental Parasitology, 2001. 97:24-34. PMID: 11207111.
136. Maher, N., H.K. Dillon, S.H. Vermund, and T.R. Unnasch, Magnetic bead capture eliminates PCR inhibitors in samples collected from the airborne environment, permitting
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detection of Pneumocystis carinii DNA. Applied and Environmental Microbiology, 2001. 67:449-452. PMID: 11133478.
137. Maher, N.H., S.H. Vermund, D.A. Welsh, H.K. Dillon, A. Awooda, and T.R. Unnasch, Development and Characterization of a Molecular Viability Assay for Pneumocystis carinii f. sp. hominis. Journal of Infectious Diseases, 2001. 183:1825-1827. PMID: 11372040.
138. Merriweather, A., V. Guenzler, M. Brenner, and T.R. Unnasch, Characterization and expression of enzymatically active recombinant filarial prolyl 4-hydroxylase. Molecular and Biochemical Parasitology, 2001. 116:185-97.
139. Yung, S., T.R. Unnasch, and N. Lang-Unnasch, Analysis of apicoplast targeting and transit peptide processing in Toxoplasma gondii by deletional and insertional mutagenesis. Molecular and Biochemical Parasitology, 2001. 118:11-21. PMID: 11704269.
140. Higazi, T.B., D.A. Boakye, M.D. Wilson, B.M. Mahmoud, O.Z. Baraka, M.M. Mukhtar, and T.R. Unnasch, Cytotaxonomic and Molecular Analysis of (Simulium (Edwardsellum) damnosum Theobald sensu lato from Abu Hamed, Sudan. Journal of Medical Entomology, 2000. 37:547-553. PMID: 10916295.
141. Maher, N., S. Vermund, M. Lasbury, C.H. Lee, M. Bartlett, and T.R. Unnasch, Development and evaluation of a molecular viability assay for Pneumocystis carinii. Journal of Clinical Microbiology, 2000. 38:1947-1952. PMID: 10790126.
142. Simon, C., J. Tang, S. Dalwadi, G. Staley, J. Deniega, and T.R. Unnasch, Allochronic speciation suggested by restricted gene flow between 13-year cicadas and "17-year cicadas with 13-year life cycles". Evolution, 2000. 54:1326-1336.
143. Tawe, W., E. Pearlman, T.R. Unnasch, and S. Lustigman, Angiogenic activity of Onchocerca volvulus recombinant proteins similar to vespid venom antigen 5. Molecular and Biochemical Parasitology, 2000. 109:91-99.
144. Toe, L., A.G. Adjami, B.A. Boatin, C. Back, E.S. Alley, N. Dembele, P.G. Brika, E. Pearlman, and T.R. Unnasch, Topical application of diethylcarbamazine to detect onchocerciasis recrudescence in west Africa. Transactions of The Royal Society of Tropical Medicine and Hygiene, 2000. 94:519-25.
145. Boakye, D., J.M. Tang, P. Truc, A. Merriweather, and T.R. Unnasch, Identification of blood meals in hematophagous diptera by polymerase chain reaction amplification and heteroduplex analysis. Medical and Veterinary Entomology, 1999. 13:282-287.
146. Graham, S.P., Y. Wu, K. Henkle-Duehrsen, S. Lustigman, T.R. Unnasch, G. Braun, S.A. Willimas, J. McCarthy, A.J. Trees, and A.E. Bianco, Patterns of Onchocerca volvulus recombinant antigen recognition in a bovine model of onchocerciasis. Parasitology, 1999. 119:603-612.
147. Keddie, E.M., T. Higazi, D. Boakye, A. Merriweather, M.C. Wooten, and T.R. Unnasch, Onchocerca volvulus: Limited heterogeneity in the nuclear and mitochondrial genomes. Experimental Parasitology, 1999. 93:198-206. PMID: 10600445.
148. Ogunrinade, A., D. Boakye, A. Merriweather, and T.R. Unnasch, Distribution of the blinding and non-blinding strains of Onchocerca volvulus in Nigeria. Journal of Infectious Diseases, 1999. 179:1577-1579.
149. Pearlman, E., L. Toe, B.A. Boatin, A.A. Gilles, A.W. Higgins, and T.R. Unnasch, Eotaxin expression in Onchocerca volvulus-induced dermatitis after topical application of diethylcarbamazine. Journal of Infectious Diseases, 1999. 180:1394-1397.
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150. Rodríguez-Pérez, M.A., R. Danis-Lozano, M.H. Rodríguez, T.R. Unnasch, and J.E. Bradley, Detection of Onchocerca volvulus infection in Simulium ochraceum sensu lato: Comparison of a PCR assay and fly dissection in a Mexican hypoendemic community. Parasitology, 1999. 119:613-619.
151. Unnasch, T.R., J. Bradley, J. Beauchamp, R. Tuan, and M.W. Kennedy, Characterization of a putative nuclear receptor from Onchocerca volvulus. Molecular and Biochemical Parasitology, 1999. 104:259-269. PMID: 10593180.
152. Yameogo, L., L. Toe, J.M. Hougard, B.A. Boatin, and T.R. Unnasch, Pool screen polymerase chain reaction for estimating the prevalence of Onchocerca volvulus infection in Simulium damnosum sensu lato: results of a field trial in an area subject to successful vector control. American Journal of Tropical Medicine and Hygiene, 1999. 60:124-8.
153. Keddie, E.M., T. Higazi, and T.R. Unnasch, The mitochondrial genome of Onchocerca volvulus: Sequence, structure and phylogenetic analysis. Molecular and Biochemical Parasitology, 1998. 95:111-127. PMID: 9763293.
154. Tang, J., J.K. Moulton, K. Pruess, E.W. Cupp, and T.R. Unnasch, Genetic variation in North American black flies in the subgenus Psilopelmia (Diptera:Simuliidae). Canadian Journal of Zoology, 1998. 76:205-211.
155. Toe, L., B.A. Boatin, A. Adjami, C. Back, A. Merriweather, and T.R. Unnasch, Detection Of Onchocerca volvulus Infection By O-150 Polymerase Chain Reaction Analysis Of Skin Scratches. Journal of Infectious Diseases, 1998. 178:282-285.
156. Pearlman, E., E. Diaconu, F.E. Hazlett, A. Merriweather, and T.R. Unnasch, Identification of an epitope of a recombinant Onchocerca volvulus protein that induces corneal pathology. Molecular and Biochemical Parasitology, 1997. 89:123-135.
157. Tang, J. and T.R. Unnasch, Heteroduplex analysis in medical entomology: A rapid and sensitive sequence-based tool for population and phylogenetic studies. Parasitology Today, 1997. 13:271-274.
158. Toe, L., C. Back, A.G. Adjami, J.M. Tang, and T.R. Unnasch, Onchocerca volvulus: Comparison of field collection methods for the preservation of parasite and vector samples for PCR analysis. Bulletin of the World Health Organization, 1997. 75:443-447.
159. Toe, L., J. Tang, C. Back, C.R. Katholi, and T.R. Unnasch, Vector-Parasite transmission complexes for onchocerciasis in West Africa. The Lancet, 1997. 349:163-166.
160. Chakravarti, B., B.M. Greene, S.K. Dey, J.S. Parker, T.R. Unnasch, T.A. Herring, A. Gbakima, M.L. Eberhard, and D.N. Chakravarti, Onchocerca volvulus: Expression and Purification of Recombinant Antigen RAL-2 - Studies on Immunogenicity and Pathogenicity. Biochemical Archives, 1996. 12:55-69.
161. Katholi, C.R., A. Merriweather, and T.R. Unnasch, An Analysis of Variance Type Test for Comparing Clusters of DNA Sequences Based on Randomization Test Methodologies. Journal of Applied Statistics, 1996. 24:371-381.
162. Merriweather, A. and T.R. Unnasch, Onchocerca volvulus: Development of a species specific polymerase chain reaction assay. Experimental Parasitology, 1996. 83:164-166.
163. Tang, J., K. Pruess, E.W. Cupp, and T.R. Unnasch, Molecular phylogeny and typing of blackflies (Diptera:Simuliidae) that serve as vectors of human or bovine onchocerciasis. Medical and Veterinary Entomology, 1996. 10:228-234.
164. Tang, J., L. Toe, C. Back, and T.R. Unnasch, Intra-specific Heterogeneity of the rDNA Internal Transcribed Spacer in the Simulium damnosum (Diptera:Simuliidae) Complex. Molecular Biology and Evolution, 1996. 13:244-252.
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165. Tang, J., K. Pruess, and T.R. Unnasch, Genotyping North American black flies by means of mitochondrial ribosomal RNA sequences. Canadian Journal of Zoology, 1996
. 74:39-46. 166. Yates, R.A., R.S. Tuan, K.J. Shepley, and T.R. Unnasch, Characterization of genes
encoding members of the nuclear hormone receptor superfamily from Onchocerca volvulus. Molecular and Biochemical Parasitology, 1995. 70:19-31. PMID: 7637701.
167. Katholi, C.R., L. Toe, A. Merriweather, and T.R. Unnasch, Determining the prevalence of Onchocerca volvulus infection in vector populations by polymerase chain reaction screening of pools of black flies. Journal of Infectious Diseases, 1995. 172:1414-7.
168. Tang, J. and T.R. Unnasch, Discriminating PCR artifacts using DHDA (directed heteroduplex analysis). BioTechniques, 1995. 19:902-905.
169. Tang, J., L. Toe, C. Back, and T.R. Unnasch, Mitochondrial alleles of Simulium damnosum sensu lato infected with Onchocerca volvulus. International Journal for Parasitology, 1995. 25:1251-4.
170. Zimmerman, P.A., M. Shapiro, J. Tang, T.B. Nutman, and T.R. Unnasch, Optimizing probe selection in directed heteroduplex analysis using HDProbe 1.1. BioTechniques, 1995. 19:972-977.
171. Tang, J., L. Toe, C. Back, P.A. Zimmerman, K. Pruess, and T.R. Unnasch, The Simulium damnosum species complex: phylogenetic analysis and molecular identification based upon mitochondrially encoded gene sequences. Insect Molecular Biology, 1995. 4:79-88.
172. Raghavan, N., D.O. Freedman, P.C. Fitzgerald, T.R. Unnasch, E.A. Ottesen, and T.B. Nutman, Cloning and characterization of a potentially protective chitinase-like recombinant antigen from Wuchereria bancrofti. Infection and Immunity, 1994. 62:1901-1908.
173. Toe, L., A. Merriweather, and T.R. Unnasch, DNA probe based classification of Simulium damnosum s.l. borne and human derived filarial parasites in the Onchocerciasis Control Programme area. American Journal of Tropical Medicine and Hygiene, 1994. 51:676-683.
174. LeBlanc, C., C.R. Katholi, T.R. Unnasch, and S.I. Hruska. DNA Sequence Analysis using Hierarchical ART-based Classification Networks. in Proceedings of the 2nd International Conference on Intelligent Systems in Molecular Biology. 1994. Stanford University.
175. Rokeach, L.A., P.A. Zimmerman, and T.R. Unnasch, Epitopes of the Onchocerca volvulus RAL-1 antigen, a member of the calreticulin family of proteins, recognized by sera from patients with onchocerciasis. Infection and Immunity, 1994. 62:3696-3704.
176. Pearlman, E., J.W. Kazura, D.S. Bardenstein, T.R. Unnasch, F. Perler, E. Diaconu, F.E. Hazlett, and J.H. Lass, Induction of Stromal Keratitis by Recombinant Onchocerca volvulus proteins Ov33, RAL2 and RAL6, in Advances in Ocular Immunology, R.B. Nussenblatt, et al., Editors. 1994, Elsevier: Amsterdam. p. 289-292.
177. Zhou, Y., E. Dziak, T.R. Unnasch, and M. Opas, Major retinal cell components recognized by onchocerciasis sera are associated with the cell surface and nucleoli. Investigative Ophthamology and Vision Science, 1994. 35:1089-1099.
178. Wilson, W.R., R.S. Tuan, K.J. Shepley, D.O. Freedman, B.M. Greene, K. Awadzi, and T.R. Unnasch, The Onchocerca volvulus homologue of the multifunctional polypeptide protein disulfide isomerase. Molecular and Biochemical Parasitology, 1994. 68:103-117.
179. Zimmerman, P.A., C.R. Katholi, M.C. Wooten, N. Lang-Unnasch, and T.R. Unnasch,
31
Recent evolutionary history of American Onchocerca volvulus, based on analysis of a tandemly repeated DNA sequence family. Molecular Biology and Evolution, 1994. 11:384-392.
180. Freedman, D.O., T.R. Unnasch, A. Merriweather, and K. Awadzi, Truly infection free persons are rare in areas hyperendemic for African onchocerciasis. Journal of Infectious Diseases, 1994. 170:1054-1055.
181. Zimmerman, P.A., L. Toe, and T.R. Unnasch, Design of Onchocerca DNA probes based upon analysis of a repeated sequence family. Molecular and Biochemical Parasitology, 1993. 58:259-269.
182. Bradley, J.E., R.S. Tuan, K.J. Shepley, T.I.M. Tree, R.M. Maizels, R. Helm, W.F. Gregory, and T.R. Unnasch, Onchocerca volvulus: Characterization of an immunodominant antigen present in adult and larval parasites. Experimental Parasitology, 1993. 77:414-424.
183. Kron, M.A., K. Erttmann, B.M. Greene, and T. Unnasch, Characterization of a possible tRNA synthetase gene from Onchocerca volvulus. Molecular and Biochemical Parasitology, 1992. 52:289-92.
184. Zimmerman, P.A., K.Y. Dadzie, G. DeSole, J. Remme, E.S. Alley, and T.R. Unnasch, Onchocerca volvulus DNA probe classification correlates with epidemiological patterns of blindness. Journal of Infectious Diseases, 1992. 165:964-968.
185. Unnasch, T.R., C.R. Katholi, and L.M. Coate, Onchocerca volvulus: Frequency of codon usage. Experimental Parasitology, 1992. 75:457-459.
186. Rokeach, L.A., J.A. Haselby, J.F. Meilof, R.J.T. Smeenk, T.R. Unnasch, B.M. Greene, and S.O. Hoch, Characterization of the Autoantigen Calreticulin. Journal of Immunology, 1991. 147:3031-3039.
187. Meredith, S.E.O., G. Lando, A.A. Gbakima, P.A. Zimmerman, and T.R. Unnasch, Onchocerca volvulus: Application of the Polymerase Chain Reaction to Identification and Strain Differentiation of the Parasite. Experimental Parasitology, 1991. 73:335-344. 1915748
188. Zimmerman, P.A., M.L. Spell, J. Rawls, and T.R. Unnasch, Transformation of DNA sequence data into geometric symbols. Biotechniques, 1991. 11:50-52.
189. Erttmann, K.D., S.E.O. Meredith, B.M. Greene, and T.R. Unnasch, Isolation and characterization of form specific DNA sequences of Onchocerca volvulus. Acta Leidensia, 1990. 59:253-260.
190. Joseph, J.T., S.M. Aldritt, T.R. Unnasch, O. Puijalon, and D.F. Wirth, Characterization of a conserved extra chromosomal element isolated from the avian malaria parasite Plasmodium gallinaceum. Molecular and Cellular Biochemistry, 1989. 9:3621-3629.
191. Meredith, S.E.O., T.R. Unnasch, M. Karam, W.F. Piessens, and D.F. Wirth, Cloning and characterization of an Onchocerca volvulus specific DNA sequence. Molecular and Biochemical Parasitology, 1989. 36:1-10.
192. Gallin, M.Y., M. Tan, M.A. Kron, D. Rechnitzer, B.M. Greene, H.S. Newland, A.T. White, H.R. Taylor, and T.R. Unnasch, Onchocerca volvulus recombinant antigen: Physical characterization and clinical correlates with serum reactivity. Journal of Infectious Diseases, 1989. 160:521-529.
193. Waters, A.P., T.R. Unnasch, D.F. Wirth, and T.F. McCutchan, Sequence of a small ribosomal RNA gene from Plasmodium lophurae. Nucleic Acids Research, 1989. 17:1763.
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194. Unnasch, T.R., M.Y. Gallin, P.T. Soboslay, K.D. Erttmann, and B.M. Greene, Isolation and characterization of expression cDNA clones encoding antigens of Onchocerca volvulus infective larvae. Journal of Clinical Investigation, 1988. 82:262-269.
195. Erttmann, K.D., T.R. Unnasch, B.M. Greene, E.J. Albiez, J. Boateng, A.M. Denke, J.J. Ferraroni, M. Karam, H. Schulz-Key, and P.N. Williams, A DNA sequence specific for forest form Onchocerca volvulus. Nature, 1987. 327:415-417. PMID: 3035378
196. Shah, J., L. Lamontaigne, T.R. Unnasch, D.F. Wirth, and W.F. Piessens, Characterization of a ribosomal DNA clone from Brugia malayi. Molecular and Biochemical Parasitology, 1986. 19:67-75.
197. Unnasch, T.R., M. McLafferty, and D.F. Wirth, The primary structure of the rRNA insertions of Plasmodium lophurae. Molecular and Biochemical Parasitology, 1985. 16:149-161.
198. Unnasch, T.R. and D.F. Wirth, The avian malaria Plasmodium lophurae has a small number of heterogeneous ribosomal RNA genes. Nucleic Acids Research, 1983. 11:8443-8459.
199. Unnasch, T.R. and D.F. Wirth, The cloned rRNA genes of Plasmodium lophurae: A novel rDNA structure. Nucleic Acids Research, 1983. 11:8461-8472.
200. Unnasch, T.R. and G.M. Brown, Purification and properties of dihydropterin oxidase from Drosophila melanogaster. Journal of Biological Chemistry, 1982. 257:14211-14216.
Book Chapters, Reviews and Editorials: 201. Evans, D., T.R. Unnasch, and F.O. Richards, Co-implementation of onchocerciasis and
lymphatic filariasis elimination in Africa: The time is now. The Lancet, 2015. 385:2151-2152.
202. Rodrıguez-Perez, M.A., E.E. Lara-Ramırez, O. Real-Najarro, and T.R. Unnasch, A Roadmap Followed: The Path Towards the Elimination of Onchocerciasis in Latin America, in Neglected Tropical Diseases - Latin America and the Caribbean, C. Franco-Paredes and J.I. Santos-Preciado, Editors. 2015, Springer-Verlag: Vienna. p. 156-173.
203. Gargano, L.M., J. Engel, G.C. Gray, K. Howell, J. T.F., W.K. Milhous, J.G. Morris, D.G. Mead, C. Rubin, T.R. Unnasch, C.W. Woods, and J.M. Hughes, Arbovirus diseases, southeastern United States. Emerging Infectious Diseases, 2013. 19.
204. Lok, J.B. and T.R. Unnasch, Transgenesis in animal parasitic nematodes: Strongyloides spp. and Brugia spp., in WormBook, T.C.e.R. Community, Editor. 2013.
205. Cupp, E.W., C.D. Mackenzie, and T.R. Unnasch, The importance of ivermectin to human onchocerciasis: past, present and future. Research and Reports in Tropical Medicine, 2011. 2:81-92.
206. Rodríguez-Pérez, M.A., U. T., and R.-N. O., Assessment and monitoring of onchocerciasis in Latin America. Advances in Parasitology, 2011. 77:177-197.
207. Klempner, M.S., T.R. Unnasch, and L.T. Hu, Taking a bite out of vector-transmitted infectious diseases. New England Journal of Medicine, 2007. 356:2567-9.
208. Unnasch, R.S., E.W. Cupp, and T.R. Unnasch, Host selection and its role in transmission of arboviral encephalitides, in Disease Ecology: Community Structure and Pathogen Dynamics, S.K. Collinge and C. Ray, Editors. 2005, Oxford University Press: Oxford, UK. p. 73-89.
209. Unnasch, T.R., Onchocerca volvulus, in Encyclopedia of Diagnostic Genomics and
33
Proteonomics, J.a.P.M. Fuchs, Editor. 2005, Marcel Dekker, Inc.: New York, NY. p. 943-946.
210. Beard, C.B., P. Roux, G. Nevez, P.M. Hauser, J.A. Kovacs, T.R. Unnasch, and B. Lundgren, Strain Typing Methods and Molecular Epidemiology of Pneumocystis Pneumonia. Emerging Infectious Diseases, 2004. 10:1729-1735.
211. Unnasch, T.R., River Blindness. The Lancet, 2002. 360:182-183. 212. Williams, S.A., S.J. Laney, M. Lizotte-Waniewski, L.A. Bierwert, and T.R. Unnasch,
The river blindness genome project. Trends in Parasitology, 2002. 18:86-90. 213. Unnasch, T.R. and S.A. Williams, The genomes of Onchocerca volvulus. International
Journal for Parasitology, 2000. 30:543-552. 214. Bradley, J.E. and T.R. Unnasch, Molecular approaches to the diagnosis of onchocerciasis,
in Advances in Parasitology, J.R. Baker, R. Muller, and D. Rollinson, Editors. 1996, Academic Press: New York. p. 57-106.
215. Unnasch, T.R., The 0-150 PCR: A New Breed of Test. Mectizan Program Notes, 1996. 15:2-3.
216. Unnasch, T.R. and S.E.O. Meredith, The use of degenerate primers in conjunction with strain and species oligonucleotides to classify Onchocerca volvulus, in Species Diagnostic Protocols: PCR and other nucleic acid methods, J.P. Clapp, Editor. 1996, Humana Press: Totowa, NJ. p. 293-303.
217. Unnasch, T.R. and P.A. Zimmerman, Systematics: Questions and Approaches for the Molecular Parasitologist, in Molecular Approaches to Parasitology, J.C. Boothroyd and R. Komuniecki, Editors. 1995, Wiley-Liss: New York. p. 5-20.
218. Unnasch, T.R., The Filarial Genome Project. Parasitology Today, 1994. 10:415-416. 219. Unnasch, T.R., DNA probes to identify Onchocerca volvulus. Parasitology Today, 1987.
3:377-378. 220. Greene, B.M. and T.R. Unnasch, Molecular biological approaches to research in
onchocerciasis. Journal of Infectious Diseases, 1986. 154:1024-1026. 221. Brown, G.M., G.G. Krivi, C.L. Fan, and T.R. Unnasch, The biosynthesis of pteridines in
Drosophila melanogaster, in The Chemistry and Biology of Pteridines, R. Kisliuk and G.M. Brown, Editors. 1979. p. 81-86.
Thesis: 222. Unnasch, T.R., Purification and Properties of dihydropterin oxidase from Drosophila
melanogaster. 1981, Massachusetts Institute of Technology.