toyopearl gigacap deae product information
DESCRIPTION
TOYOPEARL GigaCap DEAE is a weak anion exchanger that offers a binding capacity of over 150 g/L for bovine serum albumin, which exceeds the DBC of all other commercial available DEAE anion exchange media. Besides other applications this is of special interest for the purification of plasma proteins.TRANSCRIPT
TOYOPEARL GigaCap® DEAE-650M
A new member of the renowned TOYOPEARL GigaCap Series
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TOYOPEARL GigaCap ® Series
Full line-up of high capacity ion-exchange media
Anion-exchanger
•TOYOPEARL GigaCap Q-650M
•TOYOPEARL GigaCap DEAE-650M
Cation-exchanger
•TOYOPEARL GigaCap S-650M
•TOYOPEARL GigaCap CM-650M
Mixed-mode resin (CIEC type)
•TOYOPEARL MX-Trp-650M
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TOYOPEARL GigaCap DEAE-650M
Key features:
• High dynamic binding capacity
• DEAE-type weak anion-exchanger (AEX)
Increase productivity through transfer of traditional DEAE-type
AEX to TOYOPEARL GigaCap DEAE-650M
• Example: Factor VIII, insulin,
• TOYOPEARL GigaCap DEAE-650M has slightly stronger retention than
traditional TOYOPEARL DEAE-650M
Benefit of traditional TOYOPEARL DEAE-650S/M/C
• Sharper peaks at low salt concentration
• Larger effective pore size support separation of huge biomolecules
• Three different particle sizes (S, M & C grades) available
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Dynamic binding capacities were determined at 10% breakthrough
Column size: 6 mm ID x 4 cm
Loading buffer: 0.05 mol/L Tris-HCl buffer (pH 8.5)
Elution buffer: 0.05 mol/L Tris-HCl buffer (pH 8.5) + 0.5 mol/L NaCl
Linear velocity: 212 cm/h
Detection: UV @ 280 nm
Sample: BSA (1 g/L)
SBC Conditions:
Adsorption buffer 0.05 mol/L Tris-HCl buffer (pH8.5)
Protein concentration 10 g/L, Total protein; BSA 210 g/L-gel
TOYOPEARL GigaCap DEAE-650M BSA DBC exceeds all commercial DEAE media
Comparison of DEAE-type Anion-Exchangers
Particle size Ion-exchange Recovery (%)(um) capacity (eq/L) Static Dynamic (DBC)
TOYOPEARL GigaCap DEAE-650M 50-100 0.23 179 165 100TOYOPEARL GigaCap Q-650M 50-100 0.17 191 172 97TOYOPEARL DEAE-650M 40-90 0.11 30 25 97Capto DEAE 90 (median) 0.29-0.35 143 140 96Fractogel EMD DEAE(M) 40-90 - 52 - -
ProductBinding capacity (g/L)
Crosslinked Agarose DEAE
Tentacle type DEAE
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Dynamic binding capacities were determined at 10 % breakthrough
Column: GigaCap DEAE-650M 6 mm ID x 4 cm
Loading buffer: 0.05 mol/L Tris-HCl buffer (pH 8.5) , Flow rate: 212, 407, 623, 739 cm/hr
Elution buffer: 0.05 mol/L Tris-HCl buffer (pH8.5) + 0.5 mol/L sodium chloride
Detection: UV @ 280 nm
Sample: BSA (1 g/L)
TOYOPEARL GigaCap DEAE-650M has high binding capacity even at high flow rate
Dynamic Binding Capacity @ Various Flow
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CIP conditions
Alkaline washing solution: 0.5 mol/L NaOH
Buffer washing solution: 0.05 mol/L Tris-HCl (pH8.5) + 0.5 mol/L NaCl, Washing flow rate : 0.5 mL/min.
Alkaline washing column volume: 27 CV / cycle, washing time : 1 hr
Buffer washing column volume: 10 CV / cycle
DBC conditions
Column size : 6 mm ID X 4 cm
Binding buffer : 0.05 mol/L Tris-HCl (pH 8.5)
Sample : BSA (1 g/L), Loading flow rate : 1 mL/min. Detection : UV @ 280 nm
TOYOPEARL GigaCap DEAE-650M shows high chemical stability
GigaCap DEAE CIP Study with 0.5 M NaOH
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Column size: 7.5 mm ID x 7.5 cm
Buffer A: 0.05 mol/L Tris-HCl (pH 8.5); Buffer B: 0.05 mol/L Tris-HCl + 1.0 mol/L NaCl (pH 8.5)
Gradient: 120-min linear gradient from 0 to 100% B
Flow rate: 1.0 mL/min, Detection: UV @ 280nm
Sample:1. transferrin (2.9 g/L), 2. ovalbumin (6.5 g/L), 3. trypsin inhibitor (10.0 g/L)
Injection volume: 100 µL
Selectivity of High Capacity DEAE-type AEX (1)
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1
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Agarose based DEAE
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Column size: 7.5 mm ID x 7.5 cm
Buffer A: 0.05 mol/L Tris-HCl (pH 8.5); Buffer B: 0.05 mol/L Tris-HCl + 1.0 mol/L NaCl (pH 8.5)
Gradient: 120-min linear gradient from 0% to 100% B
Flow rate: 1.0 mL/min, Detection: UV@280nm
Sample:1. transferrin (2.9 g/L), 2. ovalbumin (6.5 g/L), 3. trypsin inhibitor (10.0 g/L)
Injection volume: 100 µL
Selectivity of High Capacity DEAE-type AEX (2)
TOYOPEARL DEAE-650M shows sharpest peaks at low salt concentration
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1
3
Agarose based DEAE
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Mechanical Stability of GigaCap DEAE-650M
Column size: 22 mm ID x 20 cm
Eluent : 0.1 mol/L NaCl
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0.05
0.10
0.15
0.20
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Colu
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Linear Velocity (cm/hr)
Brand A (agarose, DEAE)
TOYOPEARL GigaCapDEAE-650M
Crosslinked Agarose DEAE
TOYOPEARL Anion-Exchanger Map
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Selection of TOYOPEARL AEX Resins
Initial recommendation →
Resin screening →
Large volume sample →
Salt tolerant →
Low salt feedstock →
- Sharper peaks →
Antibody FT mode →
Factor VIII purification →
Acidic polysaccharide →
Oligonucleotides →
Antibiotics, small MW API →
Strong alkaline CIP (1 M NaOH) →
GigaCap series
ToyoScreen IEC-A, TS RoboColumns
GigaCap series
Q-600C AR
GigaCap series, SuperQ-650
DEAE-650,
GigaCap Q-650M/Q-600C AR
DEAE-650, GigaCap DEAE-650M
GigaCap Q-650M
TSKgel SuperQ-5PW(20)
GigaCap Q-650M, SuperQ-650
Q-600C AR
Ordering Information
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Product P/N Volume
TOYOPEARL GigaCap DEAE-650M 22865 100 mL
TOYOPEARL GigaCap DEAE-650M 22866 250 mL
TOYOPEARL GigaCap DEAE-650M 22867 1000 mL
ToyoScreen GigaCap DEAE-650M 22872 1 mL x 6
ToyoScreen GigaCap DEAE-650M 22873 5 mL x 6