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Transcription *RNA polymerase (from E. coli ) 2 ’ Core Sigma factor Holoenzyme

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Page 1: Transcription *RNA polymerase (from E. coli ) 2 ’ Core Sigma factor Holoenzyme

Transcription*RNA polymerase (from E. coli )

2 ’

CoreSigma factor

Holoenzyme

Page 2: Transcription *RNA polymerase (from E. coli ) 2 ’ Core Sigma factor Holoenzyme

Transcription

Functions of a prokaryotic promoter:

1. ON/OFF Switch

2. Speed Switch

3. Alignment

Page 3: Transcription *RNA polymerase (from E. coli ) 2 ’ Core Sigma factor Holoenzyme

Transcription Initiation

Page 4: Transcription *RNA polymerase (from E. coli ) 2 ’ Core Sigma factor Holoenzyme

Transcriptional Elongation:

Page 5: Transcription *RNA polymerase (from E. coli ) 2 ’ Core Sigma factor Holoenzyme

Termination of TranscriptionInverted Repeats and Hairpins

• The repeat at right is symmetrical around its center shown with a dot

• A transcript of this sequence is self-complementary– Bases can pair up

to form a hairpin as seen in the lower panel

Page 6: Transcription *RNA polymerase (from E. coli ) 2 ’ Core Sigma factor Holoenzyme

Termination of Transcription

*Rho-independent termination

Page 7: Transcription *RNA polymerase (from E. coli ) 2 ’ Core Sigma factor Holoenzyme

factor

Termination of Transcription*Rho-dependent termination

Page 8: Transcription *RNA polymerase (from E. coli ) 2 ’ Core Sigma factor Holoenzyme

RNA Polymerase binding

*Constitutive or basal level transcription

Page 9: Transcription *RNA polymerase (from E. coli ) 2 ’ Core Sigma factor Holoenzyme

Allosteric activation of RNA polymerase

Page 10: Transcription *RNA polymerase (from E. coli ) 2 ’ Core Sigma factor Holoenzyme

Inhibition of transcription

DNA-protein interactions

Page 11: Transcription *RNA polymerase (from E. coli ) 2 ’ Core Sigma factor Holoenzyme

Negative Control of the lac Operon

Page 12: Transcription *RNA polymerase (from E. coli ) 2 ’ Core Sigma factor Holoenzyme

Proposed CAP-cAMP Activation of lac Transcription


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