tutorial webinar #6 welcome to the sixth in a series of tutorial webinars designed to help you get...
TRANSCRIPT
Tutorial Webinar #6
Welcome to the sixth in a series of tutorial webinars designed to help you get the most out of Skyline proteomics software.
We’ll begin shortly …
Webinar Audio: Make sure you have audio during the webinar.Click on the telephone icon in the JoinMe toolbar - circled here
You then have the choice to either: Connect by phone (US and International numbers) Connect by internet – a short, temporary software download is required.
Submit Skyline Questions: Use this form to submit Skyline questions to the team – we will try to answer as many as possible:
The QA link will also be available in the chat window – to open your chat window click the bubble icon – circled here
Effective Data Processing and Interrogation with Skyline
Tutorial Webinar #6
With Brendan MacLean (Principal Developer)
Agenda Welcome from the Skyline team! Effective Data Processing and Interrogation Introduction with Brendan MacLean
Brief overview of refinement by peptide/protein differentiation
Tutorial with Brendan MacLean Effective grouped study data processing and QC Sneak peak: Group Comparison features in Skyline 3.1
Audience Q&A – submit questions to Google Form:http://tinyurl.com/QA4Skyline
Supporting a Broader Hypothesis
Quantitative Assay
Unrefined
Methods
Spectral Libraries
RT Predicti
on
Previous Measureme
nts
Targeted Method
Refinement
Targeted Method Refinement
Build Method
Run Method
Evaluate Results
Refine:1. Abundance2. Measurability3. Differentiat
ion4. Degradation5. Digestion6. Scheduling7. Optimization
etc.
Identifying Plasma Proteins with Altered Levelsin a Dahl Salt Sensitive Rat Model
7 Rats on a High Salt Diet
7 Rats on a Low Salt Diet
109 proteins reported in literature as related to heart disease
Phases of Refinement: Detection
DetectionProteins: 109
Peptides: 2,165Transitions: 12,194
DifferentiationProteins: 49
Peptides: 135Transitions: 780
Investigation
151 unscheduled methods
1 scheduled method(x 42 injection)
1 scheduled method
Verification: Skyline refinement and method development
Validation
Refinement
Refinement
Scheduling Multi-Replicate Assays 49
Proteins
135Peptides
780Transitions
6Unscheduled Injections
Prior Knowledge and Consistency Based on empirical measurement Powerful enough to be used cross-lab / cross
experiment More powerful run-to-run
Relative ion abundance Spectral and chromatogram libraries
Retention time iRT
Optimal collision energy Ion mobility
Collisional cross-section
Haptoglobin L
ibra
ry
D_
102
_RE
P1
D_
102
_RE
P2
D_
102
_RE
P3
D_
103
_RE
P1
D_
103
_RE
P2
D_
103
_RE
P3
D_
108
_RE
P1
D_
108
_RE
P2
D_
108
_RE
P3
D_
138
_RE
P1
D_
138
_RE
P2
D_
138
_RE
P3
D_
154
_RE
P1
D_
154
_RE
P2
D_
154
_RE
P3
D_
172
_RE
P1
D_
172
_RE
P2
D_
172
_RE
P3
D_
196
_RE
P1
D_
196
_RE
P2
D_
196
_RE
P3
H_
146
_RE
P1
H_
146
_RE
P2
H_
146
_RE
P3
H_
147
_RE
P1
H_
147
_RE
P2
H_
147
_RE
P3
H_
148
_RE
P1
H_
148
_RE
P2
H_
148
_RE
P3
H_
159
_RE
P1
H_
159
_RE
P2
H_
159
_RE
P3
H_
160
_RE
P1
H_
160
_RE
P2
H_
160
_RE
P3
H_
161
_RE
P1
H_
161
_RE
P2
H_
161
_RE
P3
H_
162
_RE
P1
H_
162
_RE
P2
H_
162
_RE
P3
Replicate
0
20
40
60
80
100
Pe
ak
Are
a P
erc
en
tag
e
y13 - 1426.6696+ y9 - 1024.5310+ y7 - 854.4254+ y6 - 691.3621+y4 - 477.2304+ y3 - 363.1874+
Lib
rary
D_
102
_RE
P1
D_
102
_RE
P2
D_
102
_RE
P3
D_
103
_RE
P1
D_
103
_RE
P2
D_
103
_RE
P3
D_
108
_RE
P1
D_
108
_RE
P2
D_
108
_RE
P3
D_
138
_RE
P1
D_
138
_RE
P2
D_
138
_RE
P3
D_
154
_RE
P1
D_
154
_RE
P2
D_
154
_RE
P3
D_
172
_RE
P1
D_
172
_RE
P2
D_
172
_RE
P3
D_
196
_RE
P1
D_
196
_RE
P2
D_
196
_RE
P3
H_
146
_RE
P1
H_
146
_RE
P2
H_
146
_RE
P3
H_
147
_RE
P1
H_
147
_RE
P2
H_
147
_RE
P3
H_
148
_RE
P1
H_
148
_RE
P2
H_
148
_RE
P3
H_
159
_RE
P1
H_
159
_RE
P2
H_
159
_RE
P3
H_
160
_RE
P1
H_
160
_RE
P2
H_
160
_RE
P3
H_
161
_RE
P1
H_
161
_RE
P2
H_
161
_RE
P3
H_
162
_RE
P1
H_
162
_RE
P2
H_
162
_RE
P3
Replicate
0
20
40
60
80
100
Pe
ak
Are
a P
erc
en
tag
e
y13 - 1426.6696+ y9 - 1024.5310+ y7 - 854.4254+ y6 - 691.3621+y4 - 477.2304+ y3 - 363.1874+
D_
102
_RE
P1
D_
102
_RE
P2
D_
102
_RE
P3
D_
103
_RE
P1
D_
103
_RE
P2
D_
103
_RE
P3
D_
108
_RE
P1
D_
108
_RE
P2
D_
108
_RE
P3
D_
138
_RE
P1
D_
138
_RE
P2
D_
138
_RE
P3
D_
154
_RE
P1
D_
154
_RE
P2
D_
154
_RE
P3
D_
172
_RE
P1
D_
172
_RE
P2
D_
172
_RE
P3
D_
196
_RE
P1
D_
196
_RE
P2
D_
196
_RE
P3
H_
146
_RE
P1
H_
146
_RE
P2
H_
146
_RE
P3
H_
147
_RE
P1
H_
147
_RE
P2
H_
147
_RE
P3
H_
148
_RE
P1
H_
148
_RE
P2
H_
148
_RE
P3
H_
159
_RE
P1
H_
159
_RE
P2
H_
159
_RE
P3
H_
160
_RE
P1
H_
160
_RE
P2
H_
160
_RE
P3
H_
161
_RE
P1
H_
161
_RE
P2
H_
161
_RE
P3
H_
162
_RE
P1
H_
162
_RE
P2
H_
162
_RE
P3
Replicate
16.5
17.0
17.5
18.0
Re
ten
tio
n T
ime
y13 - 1426.6696+ y9 - 1024.5310+ y7 - 854.4254+ y6 - 691.3621+y4 - 477.2304+ y3 - 363.1874+
D_
102
_RE
P1
D_
102
_RE
P2
D_
102
_RE
P3
D_
103
_RE
P1
D_
103
_RE
P2
D_
103
_RE
P3
D_
108
_RE
P1
D_
108
_RE
P2
D_
108
_RE
P3
D_
138
_RE
P1
D_
138
_RE
P2
D_
138
_RE
P3
D_
154
_RE
P1
D_
154
_RE
P2
D_
154
_RE
P3
D_
172
_RE
P1
D_
172
_RE
P2
D_
172
_RE
P3
D_
196
_RE
P1
D_
196
_RE
P2
D_
196
_RE
P3
H_
146
_RE
P1
H_
146
_RE
P2
H_
146
_RE
P3
H_
147
_RE
P1
H_
147
_RE
P2
H_
147
_RE
P3
H_
148
_RE
P1
H_
148
_RE
P2
H_
148
_RE
P3
H_
159
_RE
P1
H_
159
_RE
P2
H_
159
_RE
P3
H_
160
_RE
P1
H_
160
_RE
P2
H_
160
_RE
P3
H_
161
_RE
P1
H_
161
_RE
P2
H_
161
_RE
P3
H_
162
_RE
P1
H_
162
_RE
P2
H_
162
_RE
P3
Replicate
16.5
17.0
17.5
18.0
Re
ten
tio
n T
ime
y13 - 1426.6696+ y9 - 1024.5310+ y7 - 854.4254+ y6 - 691.3621+y4 - 477.2304+ y3 - 363.1874+
LQTEGDGIYTLNSEK
Diseased Healthy
Haptoglobin L
ibra
ry
D_
102
_RE
P1
D_
102
_RE
P2
D_
102
_RE
P3
D_
103
_RE
P1
D_
103
_RE
P2
D_
103
_RE
P3
D_
108
_RE
P1
D_
108
_RE
P2
D_
108
_RE
P3
D_
138
_RE
P1
D_
138
_RE
P2
D_
138
_RE
P3
D_
154
_RE
P1
D_
154
_RE
P2
D_
154
_RE
P3
D_
172
_RE
P1
D_
172
_RE
P2
D_
172
_RE
P3
D_
196
_RE
P1
D_
196
_RE
P2
D_
196
_RE
P3
H_
146
_RE
P1
H_
146
_RE
P2
H_
146
_RE
P3
H_
147
_RE
P1
H_
147
_RE
P2
H_
147
_RE
P3
H_
148
_RE
P1
H_
148
_RE
P2
H_
148
_RE
P3
H_
159
_RE
P1
H_
159
_RE
P2
H_
159
_RE
P3
H_
160
_RE
P1
H_
160
_RE
P2
H_
160
_RE
P3
H_
161
_RE
P1
H_
161
_RE
P2
H_
161
_RE
P3
H_
162
_RE
P1
H_
162
_RE
P2
H_
162
_RE
P3
Replicate
0
2
4
6
8
Pe
ak
Are
a (
10
^6
)
y13 - 1426.6696+ y9 - 1024.5310+ y7 - 854.4254+ y6 - 691.3621+ y4 - 477.2304+y3 - 363.1874+
Lib
rary
D_
102
_RE
P1
D_
102
_RE
P2
D_
102
_RE
P3
D_
103
_RE
P1
D_
103
_RE
P2
D_
103
_RE
P3
D_
108
_RE
P1
D_
108
_RE
P2
D_
108
_RE
P3
D_
138
_RE
P1
D_
138
_RE
P2
D_
138
_RE
P3
D_
154
_RE
P1
D_
154
_RE
P2
D_
154
_RE
P3
D_
172
_RE
P1
D_
172
_RE
P2
D_
172
_RE
P3
D_
196
_RE
P1
D_
196
_RE
P2
D_
196
_RE
P3
H_
146
_RE
P1
H_
146
_RE
P2
H_
146
_RE
P3
H_
147
_RE
P1
H_
147
_RE
P2
H_
147
_RE
P3
H_
148
_RE
P1
H_
148
_RE
P2
H_
148
_RE
P3
H_
159
_RE
P1
H_
159
_RE
P2
H_
159
_RE
P3
H_
160
_RE
P1
H_
160
_RE
P2
H_
160
_RE
P3
H_
161
_RE
P1
H_
161
_RE
P2
H_
161
_RE
P3
H_
162
_RE
P1
H_
162
_RE
P2
H_
162
_RE
P3
Replicate
0
2
4
6
8
Pe
ak
Are
a (
10
^6
)
y13 - 1426.6696+ y9 - 1024.5310+ y7 - 854.4254+ y6 - 691.3621+ y4 - 477.2304+y3 - 363.1874+
LQTEGDGIYTLNSEK L
ibra
ry
D_
102
_RE
P1
D_
102
_RE
P2
D_
102
_RE
P3
D_
103
_RE
P1
D_
103
_RE
P2
D_
103
_RE
P3
D_
108
_RE
P1
D_
108
_RE
P2
D_
108
_RE
P3
D_
138
_RE
P1
D_
138
_RE
P2
D_
138
_RE
P3
D_
154
_RE
P1
D_
154
_RE
P2
D_
154
_RE
P3
D_
172
_RE
P1
D_
172
_RE
P2
D_
172
_RE
P3
D_
196
_RE
P1
D_
196
_RE
P2
D_
196
_RE
P3
H_
146
_RE
P1
H_
146
_RE
P2
H_
146
_RE
P3
H_
147
_RE
P1
H_
147
_RE
P2
H_
147
_RE
P3
H_
148
_RE
P1
H_
148
_RE
P2
H_
148
_RE
P3
H_
159
_RE
P1
H_
159
_RE
P2
H_
159
_RE
P3
H_
160
_RE
P1
H_
160
_RE
P2
H_
160
_RE
P3
H_
161
_RE
P1
H_
161
_RE
P2
H_
161
_RE
P3
H_
162
_RE
P1
H_
162
_RE
P2
H_
162
_RE
P3
Replicate
0.0
0.5
1.0
1.5
2.0
Pe
ak
Are
a (
10
^6
)
y7 - 757.4818+ y6 - 658.4134+ y5 - 543.3865+ y4 - 430.3024+
Lib
rary
D_
102
_RE
P1
D_
102
_RE
P2
D_
102
_RE
P3
D_
103
_RE
P1
D_
103
_RE
P2
D_
103
_RE
P3
D_
108
_RE
P1
D_
108
_RE
P2
D_
108
_RE
P3
D_
138
_RE
P1
D_
138
_RE
P2
D_
138
_RE
P3
D_
154
_RE
P1
D_
154
_RE
P2
D_
154
_RE
P3
D_
172
_RE
P1
D_
172
_RE
P2
D_
172
_RE
P3
D_
196
_RE
P1
D_
196
_RE
P2
D_
196
_RE
P3
H_
146
_RE
P1
H_
146
_RE
P2
H_
146
_RE
P3
H_
147
_RE
P1
H_
147
_RE
P2
H_
147
_RE
P3
H_
148
_RE
P1
H_
148
_RE
P2
H_
148
_RE
P3
H_
159
_RE
P1
H_
159
_RE
P2
H_
159
_RE
P3
H_
160
_RE
P1
H_
160
_RE
P2
H_
160
_RE
P3
H_
161
_RE
P1
H_
161
_RE
P2
H_
161
_RE
P3
H_
162
_RE
P1
H_
162
_RE
P2
H_
162
_RE
P3
Replicate
0.0
0.5
1.0
1.5
2.0
Pe
ak
Are
a (
10
^6
)
y7 - 757.4818+ y6 - 658.4134+ y5 - 543.3865+ y4 - 430.3024+
SVVDIGLIK
Diseased Healthy
Mitochondrial 39S ribosomal protein L9D
_10
2_R
EP
1
D_
102
_RE
P2
D_
102
_RE
P3
D_
103
_RE
P1
D_
103
_RE
P2
D_
103
_RE
P3
D_
108
_RE
P1
D_
108
_RE
P2
D_
108
_RE
P3
D_
138
_RE
P1
D_
138
_RE
P2
D_
138
_RE
P3
D_
154
_RE
P1
D_
154
_RE
P2
D_
154
_RE
P3
D_
172
_RE
P1
D_
172
_RE
P2
D_
172
_RE
P3
D_
196
_RE
P1
D_
196
_RE
P2
D_
196
_RE
P3
H_
146
_RE
P1
H_
146
_RE
P2
H_
146
_RE
P3
H_
147
_RE
P1
H_
147
_RE
P2
H_
147
_RE
P3
H_
148
_RE
P1
H_
148
_RE
P2
H_
148
_RE
P3
H_
159
_RE
P1
H_
159
_RE
P2
H_
159
_RE
P3
H_
160
_RE
P1
H_
160
_RE
P2
H_
160
_RE
P3
H_
161
_RE
P1
H_
161
_RE
P2
H_
161
_RE
P3
H_
162
_RE
P1
H_
162
_RE
P2
H_
162
_RE
P3
Replicate
0
20
40
60
80
100
Pe
ak
Are
a P
erc
en
tag
e
y8 - 930.4944+ y7 - 744.4151+ y6 - 673.3780+ y5 - 616.3566+ y4 - 545.3194+y3 - 474.2823+
D_
102
_RE
P1
D_
102
_RE
P2
D_
102
_RE
P3
D_
103
_RE
P1
D_
103
_RE
P2
D_
103
_RE
P3
D_
108
_RE
P1
D_
108
_RE
P2
D_
108
_RE
P3
D_
138
_RE
P1
D_
138
_RE
P2
D_
138
_RE
P3
D_
154
_RE
P1
D_
154
_RE
P2
D_
154
_RE
P3
D_
172
_RE
P1
D_
172
_RE
P2
D_
172
_RE
P3
D_
196
_RE
P1
D_
196
_RE
P2
D_
196
_RE
P3
H_
146
_RE
P1
H_
146
_RE
P2
H_
146
_RE
P3
H_
147
_RE
P1
H_
147
_RE
P2
H_
147
_RE
P3
H_
148
_RE
P1
H_
148
_RE
P2
H_
148
_RE
P3
H_
159
_RE
P1
H_
159
_RE
P2
H_
159
_RE
P3
H_
160
_RE
P1
H_
160
_RE
P2
H_
160
_RE
P3
H_
161
_RE
P1
H_
161
_RE
P2
H_
161
_RE
P3
H_
162
_RE
P1
H_
162
_RE
P2
H_
162
_RE
P3
Replicate
0
20
40
60
80
100
Pe
ak
Are
a P
erc
en
tag
e
y8 - 930.4944+ y7 - 744.4151+ y6 - 673.3780+ y5 - 616.3566+ y4 - 545.3194+y3 - 474.2823+
D_
102
_RE
P1
D_
102
_RE
P2
D_
102
_RE
P3
D_
103
_RE
P1
D_
103
_RE
P2
D_
103
_RE
P3
D_
108
_RE
P1
D_
108
_RE
P2
D_
108
_RE
P3
D_
138
_RE
P1
D_
138
_RE
P2
D_
138
_RE
P3
D_
154
_RE
P1
D_
154
_RE
P2
D_
154
_RE
P3
D_
172
_RE
P1
D_
172
_RE
P2
D_
172
_RE
P3
D_
196
_RE
P1
D_
196
_RE
P2
D_
196
_RE
P3
H_
146
_RE
P1
H_
146
_RE
P2
H_
146
_RE
P3
H_
147
_RE
P1
H_
147
_RE
P2
H_
147
_RE
P3
H_
148
_RE
P1
H_
148
_RE
P2
H_
148
_RE
P3
H_
159
_RE
P1
H_
159
_RE
P2
H_
159
_RE
P3
H_
160
_RE
P1
H_
160
_RE
P2
H_
160
_RE
P3
H_
161
_RE
P1
H_
161
_RE
P2
H_
161
_RE
P3
H_
162
_RE
P1
H_
162
_RE
P2
H_
162
_RE
P3
Replicate
19.5
20.0
20.5
21.0
21.5
22.0
22.5
Re
ten
tio
n T
ime
y8 - 930.4944+ y7 - 744.4151+ y6 - 673.3780+ y5 - 616.3566+ y4 - 545.3194+y3 - 474.2823+
D_
102
_RE
P1
D_
102
_RE
P2
D_
102
_RE
P3
D_
103
_RE
P1
D_
103
_RE
P2
D_
103
_RE
P3
D_
108
_RE
P1
D_
108
_RE
P2
D_
108
_RE
P3
D_
138
_RE
P1
D_
138
_RE
P2
D_
138
_RE
P3
D_
154
_RE
P1
D_
154
_RE
P2
D_
154
_RE
P3
D_
172
_RE
P1
D_
172
_RE
P2
D_
172
_RE
P3
D_
196
_RE
P1
D_
196
_RE
P2
D_
196
_RE
P3
H_
146
_RE
P1
H_
146
_RE
P2
H_
146
_RE
P3
H_
147
_RE
P1
H_
147
_RE
P2
H_
147
_RE
P3
H_
148
_RE
P1
H_
148
_RE
P2
H_
148
_RE
P3
H_
159
_RE
P1
H_
159
_RE
P2
H_
159
_RE
P3
H_
160
_RE
P1
H_
160
_RE
P2
H_
160
_RE
P3
H_
161
_RE
P1
H_
161
_RE
P2
H_
161
_RE
P3
H_
162
_RE
P1
H_
162
_RE
P2
H_
162
_RE
P3
Replicate
19.5
20.0
20.5
21.0
21.5
22.0
22.5
Re
ten
tio
n T
ime
y8 - 930.4944+ y7 - 744.4151+ y6 - 673.3780+ y5 - 616.3566+ y4 - 545.3194+y3 - 474.2823+
CSSLLWAGAAWLR
Mitochondrial 39S ribosomal protein L9D
_10
2_R
EP
1
D_
102
_RE
P2
D_
102
_RE
P3
D_
103
_RE
P1
D_
103
_RE
P2
D_
103
_RE
P3
D_
108
_RE
P1
D_
108
_RE
P2
D_
108
_RE
P3
D_
138
_RE
P1
D_
138
_RE
P2
D_
138
_RE
P3
D_
154
_RE
P1
D_
154
_RE
P2
D_
154
_RE
P3
D_
172
_RE
P1
D_
172
_RE
P2
D_
172
_RE
P3
D_
196
_RE
P1
D_
196
_RE
P2
D_
196
_RE
P3
H_
146
_RE
P1
H_
146
_RE
P2
H_
146
_RE
P3
H_
147
_RE
P1
H_
147
_RE
P2
H_
147
_RE
P3
H_
148
_RE
P1
H_
148
_RE
P2
H_
148
_RE
P3
H_
159
_RE
P1
H_
159
_RE
P2
H_
159
_RE
P3
H_
160
_RE
P1
H_
160
_RE
P2
H_
160
_RE
P3
H_
161
_RE
P1
H_
161
_RE
P2
H_
161
_RE
P3
H_
162
_RE
P1
H_
162
_RE
P2
H_
162
_RE
P3
Replicate
020406080
100120140
Pe
ak
Are
a (
10
^3
)
y8 - 930.4944+ y7 - 744.4151+ y6 - 673.3780+ y5 - 616.3566+ y4 - 545.3194+y3 - 474.2823+
D_
102
_RE
P1
D_
102
_RE
P2
D_
102
_RE
P3
D_
103
_RE
P1
D_
103
_RE
P2
D_
103
_RE
P3
D_
108
_RE
P1
D_
108
_RE
P2
D_
108
_RE
P3
D_
138
_RE
P1
D_
138
_RE
P2
D_
138
_RE
P3
D_
154
_RE
P1
D_
154
_RE
P2
D_
154
_RE
P3
D_
172
_RE
P1
D_
172
_RE
P2
D_
172
_RE
P3
D_
196
_RE
P1
D_
196
_RE
P2
D_
196
_RE
P3
H_
146
_RE
P1
H_
146
_RE
P2
H_
146
_RE
P3
H_
147
_RE
P1
H_
147
_RE
P2
H_
147
_RE
P3
H_
148
_RE
P1
H_
148
_RE
P2
H_
148
_RE
P3
H_
159
_RE
P1
H_
159
_RE
P2
H_
159
_RE
P3
H_
160
_RE
P1
H_
160
_RE
P2
H_
160
_RE
P3
H_
161
_RE
P1
H_
161
_RE
P2
H_
161
_RE
P3
H_
162
_RE
P1
H_
162
_RE
P2
H_
162
_RE
P3
Replicate
020406080
100120140
Pe
ak
Are
a (
10
^3
)
y8 - 930.4944+ y7 - 744.4151+ y6 - 673.3780+ y5 - 616.3566+ y4 - 545.3194+y3 - 474.2823+
D_
102
_RE
P1
D_
102
_RE
P2
D_
102
_RE
P3
D_
103
_RE
P1
D_
103
_RE
P2
D_
103
_RE
P3
D_
108
_RE
P1
D_
108
_RE
P2
D_
108
_RE
P3
D_
138
_RE
P1
D_
138
_RE
P2
D_
138
_RE
P3
D_
154
_RE
P1
D_
154
_RE
P2
D_
154
_RE
P3
D_
172
_RE
P1
D_
172
_RE
P2
D_
172
_RE
P3
D_
196
_RE
P1
D_
196
_RE
P2
D_
196
_RE
P3
H_
146
_RE
P1
H_
146
_RE
P2
H_
146
_RE
P3
H_
147
_RE
P1
H_
147
_RE
P2
H_
147
_RE
P3
H_
148
_RE
P1
H_
148
_RE
P2
H_
148
_RE
P3
H_
159
_RE
P1
H_
159
_RE
P2
H_
159
_RE
P3
H_
160
_RE
P1
H_
160
_RE
P2
H_
160
_RE
P3
H_
161
_RE
P1
H_
161
_RE
P2
H_
161
_RE
P3
H_
162
_RE
P1
H_
162
_RE
P2
H_
162
_RE
P3
Replicate
0
200
400
600
800
Pe
ak
Are
a (
10
^3
)
y9 - 961.5942+ y8 - 862.5257+ y6 - 664.3889+ y5 - 593.3518+ y4 - 496.2990+y3 - 359.2401+
D_
102
_RE
P1
D_
102
_RE
P2
D_
102
_RE
P3
D_
103
_RE
P1
D_
103
_RE
P2
D_
103
_RE
P3
D_
108
_RE
P1
D_
108
_RE
P2
D_
108
_RE
P3
D_
138
_RE
P1
D_
138
_RE
P2
D_
138
_RE
P3
D_
154
_RE
P1
D_
154
_RE
P2
D_
154
_RE
P3
D_
172
_RE
P1
D_
172
_RE
P2
D_
172
_RE
P3
D_
196
_RE
P1
D_
196
_RE
P2
D_
196
_RE
P3
H_
146
_RE
P1
H_
146
_RE
P2
H_
146
_RE
P3
H_
147
_RE
P1
H_
147
_RE
P2
H_
147
_RE
P3
H_
148
_RE
P1
H_
148
_RE
P2
H_
148
_RE
P3
H_
159
_RE
P1
H_
159
_RE
P2
H_
159
_RE
P3
H_
160
_RE
P1
H_
160
_RE
P2
H_
160
_RE
P3
H_
161
_RE
P1
H_
161
_RE
P2
H_
161
_RE
P3
H_
162
_RE
P1
H_
162
_RE
P2
H_
162
_RE
P3
Replicate
0
200
400
600
800
Pe
ak
Are
a (
10
^3
)
y9 - 961.5942+ y8 - 862.5257+ y6 - 664.3889+ y5 - 593.3518+ y4 - 496.2990+y3 - 359.2401+
SVVDIGLIK
CSSLLWAGAAWLR
CSSLLWAGAAWLRH_159_REP1
20.0 20.5 21.0 21.5
Retention Time
0
1000
2000
3000
4000
5000
Inte
ns
ity
20.7
21.6
20.4 20.6 20.8 21.0 21.2 21.4
Retention Time
0
20
40
60
80
100
120
140
Inte
ns
ity
21.0
21.4
D_138_REP3
18 19 20 21 22 23 24 25
Retention Time
0
100
200
300
400
500
600
700
800
900
Inte
ns
ity
21.0
22.5
23.119.4
22.8
20.0
19.6
D_1
02_R
EP
1
D_1
03_R
EP
1
D_1
08_R
EP
1
D_1
38_R
EP
1
D_1
54_R
EP
1
D_1
72_R
EP
1
D_1
96_R
EP
1
H_1
46_R
EP
1
H_1
47_R
EP
1
H_1
48_R
EP
1
H_1
59_R
EP
1
H_1
60_R
EP
1
H_1
61_R
EP
1
H_1
62_R
EP
1
D_1
02_R
EP
2
D_1
03_R
EP
2
D_1
08_R
EP
2
D_1
38_R
EP
2
D_1
54_R
EP
2
D_1
72_R
EP
2
D_1
96_R
EP
2
H_1
46_R
EP
2
H_1
47_R
EP
2
H_1
48_R
EP
2
H_1
59_R
EP
2
H_1
60_R
EP
2
H_1
61_R
EP
2
H_1
62_R
EP
2
D_1
02_R
EP
3
D_1
03_R
EP
3
D_1
08_R
EP
3
D_1
38_R
EP
3
D_1
54_R
EP
3
D_1
72_R
EP
3
D_1
96_R
EP
3
H_1
46_R
EP
3
H_1
47_R
EP
3
H_1
48_R
EP
3
H_1
59_R
EP
3
H_1
60_R
EP
3
H_1
61_R
EP
3
H_1
62_R
EP
3
Replicate
19.5
20.0
20.5
21.0
21.522.0
22.5
Re
ten
tio
n T
ime
y8 - 930.4944+ y7 - 744.4151+ y6 - 673.3780+ y5 - 616.3566+ y4 - 545.3194+y3 - 474.2823+
D_1
02_R
EP
1
D_1
03_R
EP
1
D_1
08_R
EP
1
D_1
38_R
EP
1
D_1
54_R
EP
1
D_1
72_R
EP
1
D_1
96_R
EP
1
H_1
46_R
EP
1
H_1
47_R
EP
1
H_1
48_R
EP
1
H_1
59_R
EP
1
H_1
60_R
EP
1
H_1
61_R
EP
1
H_1
62_R
EP
1
D_1
02_R
EP
2
D_1
03_R
EP
2
D_1
08_R
EP
2
D_1
38_R
EP
2
D_1
54_R
EP
2
D_1
72_R
EP
2
D_1
96_R
EP
2
H_1
46_R
EP
2
H_1
47_R
EP
2
H_1
48_R
EP
2
H_1
59_R
EP
2
H_1
60_R
EP
2
H_1
61_R
EP
2
H_1
62_R
EP
2
D_1
02_R
EP
3
D_1
03_R
EP
3
D_1
08_R
EP
3
D_1
38_R
EP
3
D_1
54_R
EP
3
D_1
72_R
EP
3
D_1
96_R
EP
3
H_1
46_R
EP
3
H_1
47_R
EP
3
H_1
48_R
EP
3
H_1
59_R
EP
3
H_1
60_R
EP
3
H_1
61_R
EP
3
H_1
62_R
EP
3
Replicate
19.5
20.0
20.5
21.0
21.522.0
22.5
Re
ten
tio
n T
ime
y8 - 930.4944+ y7 - 744.4151+ y6 - 673.3780+ y5 - 616.3566+ y4 - 545.3194+y3 - 474.2823+
Truncated and Missing PeaksTGTNLMDFLSR
Tutorial Effective Data Processing and Interrogation
with Skyline
Learn More NEW! Grouped Studies Tutorial
Webinar #7: iRT or Isotope Labeled Standards Tuesday, May 12
Workshop and ASMS Skyline User Group Meeting at ASMS
May 31 at Old Post Office, St. Louis, MO Workshop in Rio de Janiero, August 31-
September 2 Workshop in Puerto Vallarta, November Weeklong Course at PRBB, Barcelona,
November 15-20
Questions? Ask any questions you have on this month’s
topic, Effective Data Processing, at the following form:
http://tinyurl.com/QA4Skyline
Take the post-webinar survey
http://tinyurl.com/Survey4Webinar
Tutorial Webinar #6
This ends this Skyline Tutorial Webinar.
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A recording of today’s meeting will be available shortly at the Skyline website.
We look forward to seeing you at a future Skyline Tutorial Webinar.