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1 Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques Leontino Taveira Head of Technical Affairs and Regional Development (Latin America and the Caribbean) UPOV 32 st ISTA CONGRESS

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Page 1: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

1

Hyderabad India June 29 2019

UPOV Guidance on Molecular Techniques

Leontino Taveira Head of Technical Affairs and Regional Development

(Latin America and the Caribbean) UPOV

32st ISTA CONGRESS

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

3

Criteria to be satisfied bull NOVELTY bull DISTINCTNESS bull UNIFORMITY bull STABILITY bull VARIETY DENOMINATION bull FORMALITIES bull PAYMENT OF FEES

ldquoDUSrdquo

CONDITIONS FOR GRANTING A BREEDERrsquoS RIGHT

NO OTHER CONDITIONS

4

IMPORTANCE OF HARMONIZED APPROACH WITHIN UPOV

rArr To facilitate cooperation in DUS testing eg purchase of DUS reports

rArr To establish internationally recognized variety descriptions (effective protection)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

6

Technical Working Party

on Automation

and Computer Programs

(TWC)

COUNCIL

ADMINISTRATIVE AND LEGAL COMMITTEE (CAJ) TECHNICAL COMMITTEE (TC)

CONSULTATIVE COMMITTEE

Technical Working Party

for Vegetables

(TWV)

Technical Working Party

for Ornamental Plants and

Forest Trees

(TWO)

Technical Working Party

for Fruit Crops

(TWF)

Technical Working Party

for Agricultural

Crops

(TWA)

Office of the Union

Working Group on Biochemical and

Molecular Techniques and DNA-Profiling in Particular (BMT)

Document reference Title

TGP15 Guidance on the Use of Biochemical and Molecular Markers in the Examination of Distinctness Uniformity and Stability (DUS)

UPOVINF181 Possible Use of Molecular Markers in the Examination of Distinctness Uniformity and Stability (2011)

Document reference Title

UPOVINF171 Guidelines for DNA Profiling Molecular Marker Selection and Database Construction (ldquoBMT Guidelinesrdquo) (2010)

STATUS OF UPOV DOCUMENTS CONCERNING MOLECULAR TECHNIQUES

Calibrated molecular distances in the management of variety collections

8

02468

10121416182022242628303234363840

0 005 01 015 02 025 03 035 04 045

GA

IumlA D

ista

nces

(Tra

ditio

nal c

hara

cter

istic

di

stan

ces)

Rogers Distances (Molecular marker distances)

GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998

Type 3 Type 1

Type 4Type 2Express - Lady

Duetol - TapidorEurol - Zorro

Ceres - Cheyenne

Express - Zorro

Zorro - Apex

Goeland - Lady

Apex - GoelandApex - Lady

Eurol - Idol

Bristol - Idol

Andol - IdolBristol - Eurol Apex - Express

Bristol - Prestol Andol - Eurol Express - Goeland

Distinctness plus (Traditional characteristics)

Distinctness plus (Molecular markers)

Type 1 Yes Yes Type 2 No No Type 3 Yes No Type 4 No Yes

Graph96

Express-Falcon
Falcon-Lady
Bristol-Falcon
Doublol-Cheyenne
Akamar-Apex
Apex-Goeland
Envol-Idol
Goeland-Lady
Apex-Lady
Bristol-Idol
Eurol-Prestol
Andol-Envol
Bristol-Envol
Idol-Goeland
Apex-Bristol
Apex-Idol
Bristol--Lady
Bristol-Goeland
34 9 varieacuteteacutes preacutesentes
193
759
14 8 varieacuteteacutes preacutesentes
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19951996
23
14
3
14
11
10
20
16
9
24
11
18
21
19
12
22
6
13
6
27
10
32
24
14
20
19
16
23
14
19
19
22
21
14
22
19
13
11
16
21
18
19
11
19
17
19
22
26
25
17
19
21
13
24
14
14
14
9
20
9
11
22
14
21
16
5
10
8
10
16
14
6
16
29
20
14
16
16
23
19
18
3
19
14
4
18
11
16
14
12
19
12
8
13
11
6
16
0
5
1
26
8
19
20
10
20
20
9
14
19
9
4
20
13
15
19
11
16
11
2
11
7
5
16
4
1
5
23
19
18
13
16
16
17
10
11
22
20
18
20
18
13
10
13
23
17
22
24
24
20
25
18
20
18
14
13
23
18
20
18
11
20
10
21
9
11
13
18
10
19
19
17
8
16
14
13
14
20
11
12
14
28
16
24
26
14
19
17
23
20
14
11
16
15
13
10
20
14
3
10
10
17
20
15
14
15
15
19
20
11
19
19
17
14
14
22
16
22
22
14
19
10
19
20
10
20
16
20
20
19
18
25
14
19
12
16
16
17
10
26
8
14
13
11
11
16
12
11
17
8
10
16
19
13
13
18
22
21
12
12
10
15
23
16
26
16
22
14
11
26
18
16
5
8
2
22
16
10
20
11
14
13
13
8
9
15
6
1
11
24
18
15
12
17
17
18
13
9
19
14
12
11
13
19
14
10
18
19
11
24
16
14
21
12
8
14
19
12
26
13
22
21
17
11
24
13
21
19
16
23
17
3
20
18
13
14
21
25
24
12
19
15
22
16
16
23
17
19
15
17
25
21
21
10
13
11
24
14
17
20
24
21
18
13
8
8
18
11
14
12
26
23
3
20
14
20
20
16
19
18
8
11
7
24
13
20
19
16
25
22
8
8
4
15
11
9
8
31
19
13
14
10
24
23
10
12
19
10
6
5
20
14
15
18
14
24
21
9
8
4
16
6
8
5
29
12
10
14
7
26
23
7
11
12
12
14
15
15
16
16
16
16
16
16
16
16
18
20
21
21
21
22
22
22
23
24
25
25
27
6
19
14
0
4
18
11
15
14
12
19
12
6
11
11
6
16
5
1
26
11
18
18
13
17
20
9
13
17
6
5
1
20
12
15
19
8
15
10
1
14
9
8
16
5
8
23
20
20
13
19
16
17
12
6
19
16
1
5
18
14
19
12
14
22
15
11
12
8
5
16
1
8
26
9
16
20
9
20
20
6
27
22
29
26
23
14
28
20
16
19
16
23
24
26
31
29
27
26
23
26
25
26
19
33
19
12
27
10
26
20
8
19
13
16
11
16
12
16
16
18
23
19
12
23
11
20
9
25
24
22
13
16
25
14
32
25
14
19
18
23
24
19
17
26
23
26
15
3
13
10
22
18
20
16
26
24
14
19
18
24
20
24
17
16
20
13
18
26
19
10
13
17
16
12
20
14
14
22
18
13
20
19
22
14
28
9
21
13
14
19
16
10
16
20
14
17
26
22
19
22
17
14
10
7
16
13
19
9
33
13
19
28
25
22
15
20
21
23
20
16
18
19
14
8
21
15
14
17
20
24
26
24
17
16
20
19
16
18
9
25
13
20
19
13
19
20
17
11
17
14
14
17
17
11
18
20
23
23
21
20
17
20
12
25
24
21
22
13
25
16
24
18
9
10
20
23
22
13
11
25
26
13
16
10
7
21
9
12
6
27
14
20
13
15
20
25

Graph97

Apex-Goeland
Eurol-Prestol
Eurol-Andol
Apex-Envol
Apex-Express
Envol-Lady
Akamar-Doublol
Contact-Navajo
Ceres-Goeland
Ceres-Lady
Apex-Ceres
Columbus-Tapidor
Goeland-Lady
Apex-Lady
212
16 8 varieacuteteacutes preacutesentes
16 10 varieacuteteacutes preacutesentes
756
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19961997
18
13
13
18
9
17
24
9
6
4
8
13
15
13
9
10
8
13
10
20
8
15
16
20
6
12
13
18
13
19
14
19
19
15
24
24
20
17
22
17
18
22
17
20
17
17
12
18
18
21
11
20
7
24
13
13
15
18
21
20
18
22
18
18
19
9
3
12
18
15
18
13
9
15
18
9
19
18
18
13
19
13
19
15
19
15
4
12
15
12
16
16
5
14
2
8
8
3
17
6
25
10
16
23
10
15
15
8
18
14
18
19
19
17
15
17
13
22
13
21
16
18
19
21
10
9
11
15
17
16
15
26
14
10
19
9
19
21
15
19
25
26
15
13
9
9
22
23
16
11
16
15
19
17
23
14
21
17
18
11
17
15
17
19
20
4
17
25
10
21
11
15
26
12
10
12
10
11
5
16
5
24
18
17
24
20
18
15
21
24
15
18
12
15
26
10
18
20
22
23
21
13
15
20
13
10
16
12
15
20
13
18
11
17
12
18
9
24
22
15
17
15
21
18
14
12
15
23
22
15
22
17
9
20
20
16
11
18
12
24
5
19
16
6
24
18
12
13
13
11
20
14
7
8
13
14
18
6
11
10
8
8
14
3
16
15
21
12
13
20
4
20
18
16
22
9
15
22
12
7
10
19
18
20
9
10
16
17
15
14
10
18
16
23
10
15
22
8
17
19
16
13
9
26
23
15
8
10
17
23
19
12
22
19
9
14
13
9
23
12
25
15
13
18
13
22
9
5
21
22
12
21
23
13
19
17
11
10
12
11
8
10
1
20
12
16
24
21
19
19
21
15
17
3
14
16
23
10
13
22
14
18
23
11
16
13
12
14
10
8
17
11
2
19
20
20
17
26
13
18
12
2
18
16
12
15
15
18
20
19
10
16
15
15
8
18
11
29
9
18
22
8
18
19
6
9
22
18
8
19
11
10
20
22
6
9
12
12
13
15
13
11
11
7
22
7
16
22
18
19
15
22
10
17
15
8
21
16
11
13
17
11
10
22
11
12
15
13
8
17
11
23
11
13
19
20
16
19
22
8
20
18
3
10
15
5
10
9
10
16
19
8
14
8
11
8
15
9
23
11
15
21
19
12
12
10
13
17
13
17
9
19
16
16
20
8
17
9
10
10
18
11
17
15
8
10
8
11
10
24
19
15
23
10
17
9
6
11
17
5
12
20
8
15
14
1
8
11
7
11
9
8
17
13
17
20
21
15
10
16
20
12
15
25
15
23
24
15
16
14
14
13
20
17
29
22
23
23
10
17
15
17
14
23
19
13
30
8
18
18
10
17
14
18
20
11
3
10
9
12
11
9
7
11
11
8
13
15
6
10
15
15
20
19
15
18
9
16
16
21
17
13
18
16
18
23
16
2
18
16
13
15
11
17
17
6
13
20
18
20
25
16
21
19
23
15
17
24
18
12
15
16
12
24
19
22
22
19
21
10
20
14
10
13
26
9
24
21
20
11
18
10
26
18
20
11
24
21
23
25
21
20
8
18
20
19
24
21
23
15
20
26
26
15
14
6
20
18
15
14
11
18
17
5
12
10
15
19
20
18
19
16
12
19
15
19
15
18
9
26
16
14
12
7
13
15
10
17
15
12
19
13
15
13
19
17
19
15
19
12
15
10
13
20
20
24
15
16
21
13
24
19
8
19
15
21
18
16
20
22
18
21
26
6
22
22
10
23
16
30
19
25
21
14
14
21

Graph98

Type 3
Type 1
Type 4
Type 2
Express - Lady
Duetol - Tapidor
Eurol - Zorro
Ceres - Cheyenne
Express - Zorro
Zorro - Apex
Goeland - Lady
Apex - Goeland
Apex - Lady
Eurol - Idol
Bristol - Idol
Andol - Idol
Bristol - Eurol
Apex - Express
Bristol - Prestol
Andol - Eurol
Express - Goeland
Rogers Distances (Molecular marker distances)
GAIumlA Distances(Traditional characteristic distances)
GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998
24
10
12
28
7
12
16
24
14
19
19
18
18
17
11
20
12
19
17
23
6
28
27
17
17
14
17
24
19
15
15
30
11
13
29
30
20
18
20
15
12
24
25
18
17
15
20
29
22
19
13
20
14
17
10
19
8
8
25
9
10
21
24
26
15
12
5
15
9
20
9
1
11
24
15
17
15
9
24
17
11
12
15
8
10
23
7
6
22
21
24
9
11
8
2
10
24
1
7
1
20
15
19
19
8
12
18
5
28
15
8
10
32
14
10
15
32
27
11
9
2
8
16
29
10
1
7
22
29
5
7
10
12
25
8
7
30
25
23
32
25
28
31
17
18
21
30
27
27
20
34
23
26
25
24
8
29
37
30
17
24
25
12
11
9
7
14
25
4
21
22
18
8
18
11
12
11
19
10
14
10
20
18
24
17
10
18
10
13
16
13
10
6
10
28
4
17
32
19
6
10
7
7
16
25
9
6
6
20
21
16
19
7
14
15
9
24
29
21
22
15
31
21
17
28
19
7
20
21
21
20
30
25
21
23
26
34
22
26
24
11
23
19
14
30
24
21
32
17
22
32
28
16
23
29
24
24
23
32
22
24
23
14
14
30
29
30
16
14
18
19
20
26
24
27
18
18
19
19
16
15
21
22
27
31
30
29
26
28
16
21
24
27
27
10
8
20
19
18
15
9
11
21
8
6
7
23
15
7
9
10
16
24
11
10
8
14
25
16
17
9
3
14
9
18
20
12
11
9
30
18
10
20
29
21
7
7
11
23
23
16
11
12
18
22
10
17
11
13
21
7
18
15
5
8
2
27
11
7
21
24
22
9
7
12
16
22
9
1
7
17
22
8
11
9
11
16
4
17
12
15
2
8
27
12
7
21
24
27
10
11
12
12
27
5
10
1
18
21
13
17
15
8
22
4
11
24
9
10
16
20
11
16
20
23
31
16
23
16
12
16
7
13
9
29
12
25
22
11
21
24
15
20
25
20
24
29
34
19
25
30
32
30
24
23
22
27
16
19
22
22
33
26
35
32
8
32
18
26
12
18
9
1
10
23
10
9
25
22
29
11
16
9
5
7
19
8
0
25
14
21
25
10
14
20
8
19
17
1
7
1
26
14
6
21
24
26
10
11
1
10
13
22
8
8
20
22
12
14
12
16
20
7
17
15
11
1
7
25
10
6
23
23
28
8
12
7
1
9
22
0
8
22
20
19
19
9
11
21
6
23
20
24
20
22
24
20
20
26
14
16
14
18
17
18
29
33
25
20
22
25
22
21
25
13
16
17
6
29
15
15
29
8
18
21
34
14
21
25
22
22
21
12
26
14
22
20
25
26
30
22
23
19
19
28
22
17
19
5
29
24
16
22
30
24
16
10
8
13
25
35
21
12
19
22
26
7
23
14
27
13
27
19
15
19
7
37
17
19
26
29
27
17
17
11
17
22
32
25
14
19
21
30
7
20
16
23
12
17
13
9
8
10
30
10
7
24
30
27
9
11
9
15
11
8
10
12
9
25
22
23
20
22
13
13
17
20
24
12
12
17
18
14
11
16
10
3
13
11
8
21
32
14
16
11
13
23
14
16
22
20
9
14
14
17
18
25
24
10
15
23
14
8
14
21
16
22
24
18
20
20
21
16
19
27
23
13
20
17
17
17
11
5
8
25
13
9
19
18
20
9
7
4
4
15
26
8
7
6
17
19
13
12
13
9
17

Graph99

169
677
61 20 varieacuteteacutes preacutesentes
93 19 varieacuteteacutes preacutesentes
Goeland-Lady
Goeland-Apex
Envol-Idol
Apex-Lady
Ceres-Falcon
Bristol-Eurol
Apex-Envol
Andol-Eurol
Eurol-Idol
Bristol-Prestol
Doublol-Duetol
Capitol-Vivol
Eurol-Samourai
Bristol-Samourai
Akamar-Duetol
Contact-Envol
Duetol-Navajo
Apex-Contact
Prestol-Samouraiet Goeland-Idol
Andol-Zorro
Falcon-Navajo
Andol-Falcon
Falcon-Goeland
Lady-Navajo
Duetol-Lady
Express-Zorro
Contact-Goeland
Duetol-Lady
Distances de ROGERS
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19981999
1
1
1
1
0
0
11
11
1
1
2
2
13
13
5
5
8
8
8
8
6
6
5
5
3
7
3
7
8
10
8
10
8
8
5
5
15
15
15
15
6
6
6
6
5
5
13
13
1
1
14
14
18
18
3
3
2
2
3
3
15
15
12
12
13
13
8
8
12
18
12
18
17
17
20
20
12
12
24
2
24
2
5
20
2
20
5
11
2
11
13
13
1
1
20
20
3
16
3
16
15
15
2
8
8
2
8
8
11
11
12
12
15
15
2
4
4
2
8
13
8
13
17
19
19
17
13
13
11
14
11
14
13
13
12
17
8
12
7
20
20
7
17
8
8
8
11
11
9
9
9
9
12
18
12
18
8
8
15
15
15
15
10
10
5
5
8
8
20
6
6
20
13
13
2
15
15
2
6
6
10
10
16
16
14
6
14
6
15
21
15
21
7
7
21
21
20
7
7
20
15
15
7
7
14
6
6
9
2
2
14
9
10
10
16
16
15
15
13
6
13
6
11
11
19
8
10
10
22
8
22
19
5
5
4
5
4
5
5
7
5
15
5
12
15
12
7
5
0
12
6
0
6
12
22
11
14
14
13
22
11
13
10
6
20
6
20
10
4
15
1
4
1
8
11
15
8
11
18
18
13
15
13
15
13
13
5
17
5
9
3
3
15
12
15
5
12
5
17
9
12
12
14
14
8
10
13
10
13
8
23
6
12
9
6
9
12
23
14
12
10
12
14
10
14
14
16
12
13
12
10
13
10
16
12
15
13
12
9
10
9
5
15
13
2
5
2
13
10
13
4
9
17
9
4
17
10
12
9
10
12
9
16
8
8
12
8
16
12
8
13
13
6
3
6
3
11
12
3
20
12
20
14
11
3
14
18
7
7
18
19
19
13
19
19
11
13
11
3
18
2
20
28
3
20
2
28
12
18
12
5
13
8
12
13
5
8
8
12
8
13
9
13
12
9
16
12
18
18
16
10
10
16
15
16
15
14
11
14
11
10
16
8
16
10
8
10
2
8
12
2
6
11
23
10
6
19
8
11
23
12
19
6
6
10
7
8
1
9
21
9
21
7
1
10
8
18
14
18
14
17
17
8
8
9
5
14
3
9
21
5
16
13
14
16
13
21
3
10
5
5
10
13
13
4
15
4
15
5
8
8
8
5
8
5
5
17
15
17
15
10
8
7
8
10
9
20
20
7
9
15
12
10
15
18
12
18
10
15
23
15
23
12
12
19
19
14
14
14
11
11
8
14
8
17
6
17
6
13
13
10
2
2
5
18
10
5
18
10
7
10
13
15
13
15
15
13
15
13
7
2
2
8
8
14
7
10
14
7
10
11
14
11
18
14
18
13
13
16
16
15
16
16
15
9
2
11
2
9
12
12
11
12
15
12
15
8
11
12
14
14
8
12
11
11
24
11
24
13
13
13
15
16
16
22
13
15
16
22
13
15
15
16
14
13
12
14
12
10
10
15
10
6
10
15
6
9
9
13
13
10
11
11
10
14
14
10
10
17
8
7
8
8
17
8
7
8
8
12
2
2
12
17
17
12
12
18
18
18
18
5
8
5
16
16
8
14
14
14
8
22
22
13
13
14
14
9
14
9
8
13
13
11
13
11
13
9
9
12
12
9
9
13
13
10
10
13
13
6
6
11
11
17
17
9
9
15
15
9
9
6
6
12
12
24
24
8
8
12
19
12
19
9
9
12
12

Model 1 Characteristic-specific molecular markers

Example 1 gene specific marker for herbicide tolerance introduced by genetic modification On the basis that [hellip] - there is verification of the reliability of the link between the marker and the characteristic - different markers for the same characteristic are different methods for examining the same characteristic [hellip]

Fusarium race 0 ex 1 Example Characteristic-specific molecular marker with incomplete information on state of expression

Model 1 Characteristic-specific molecular markers

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

ex Maize parent lines

Model 2 Combining phenotypic and molecular distances in the management of variety collections

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

2

02

Model 2 Combining phenotypic and molecular distances in the management of variety collections (continued)

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

13

New Model 3 Genetic selection of similar varieties for the first growing cycle

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
0038
0038
0042
0042
0045
0045
0063
0063
0067
0067
0068
0068
0075
0075
0106
0106
011
011
0114
0114
0115
0115
0116
0116
0123
0123
0123
0123
0131
0131
0131
0131
0132
0132
0145
0145
0149
0149
0149
0149
017
017
0176
0176
018
018
0185
0185
0186
0186
0194
0194
0198
0198
0199
0199
02
02
0201
0201
0202
0202
0203
0203
0204
0204
0207
0207
021
021
021
021
0213
0213
0214
0214
0217
0217
0217
0217
0217
0217
0219
0219
0219
0219
0219
0219
0219
0219
022
022
0224
0224
0225
0225
0225
0225
0225
0225
0226
0226
0227
0227
0227
0227
0227
0227
0228
0228
0228
0228
0228
0228
023
023
023
023
023
023
023
023
0231
0231
0231
0231
0232
0232
0232
0232
0232
0232
0233
0233
0235
0235
0235
0235
0235
0235
0235
0235
0235
0235
0236
0236
0236
0236
0238
0238
0238
0238
0239
0239
0239
0239
0241
0241
0242
0242
0244
0244
0245
0245
0246
0246
0247
0247
0249
0249
0249
0249
025
025
0251
0251
0251
0251
0252
0252
0252
0252
0252
0252
0253
0253
0253
0253
0254
0254
0254
0254
0255
0255
0259
0259
0259
0259
0259
0259
026
026
026
026
0262
0262
0262
0262
0262
0262
0262
0262
0264
0264
0265
0265
0266
0266
0267
0267
0267
0267
0268
0268
0269
0269
0269
0269
0269
0269
0269
0269
027
027
027
027
027
027
0272
0272
0272
0272
0272
0272
0272
0272
0272
0272
0273
0273
0273
0273
0273
0273
0275
0275
0275
0275
0275
0275
0275
0275
0276
0276
0276
0276
0276
0276
0278
0278
0278
0278
0278
0278
0278
0278
0278
0278
0279
0279
0279
0279
0279
0279
028
028
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0282
0282
0282
0282
0283
0283
0283
0283
0283
0283
0284
0284
0284
0284
0284
0284
0284
0284
0285
0285
0285
0285
0285
0285
0285
0285
0286
0286
0286
0286
0286
0286
0286
0286
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0288
0288
0288
0288
0288
0288
0289
0289
0289
0289
0289
0289
029
029
029
029
029
029
029
029
0291
0291
0292
0292
0292
0292
0293
0293
0293
0293
0293
0293
0293
0293
0293
0293
0294
0294
0294
0294
0294
0294
0295
0295
0295
0295
0295
0295
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0298
0298
0298
0298
0298
0298
0298
0298
0298
0298
0299
0299
0299
0299
0299
0299
0299
0299
0299
0299
03
03
0302
0302
0302
0302
0303
0303
0303
0303
0304
0304
0304
0304
0304
0304
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0307
0307
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
031
031
031
031
031
031
0311
0311
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0313
0313
0313
0313
0314
0314
0315
0315
0315
0315
0316
0316
0316
0316
0316
0316
0318
0318
0318
0318
0318
0318
0319
0319
0319
0319
0319
0319
0319
0319
0319
0319
032
032
032
032
032
032
032
032
0321
0321
0321
0321
0322
0322
0322
0322
0323
0323
0324
0324
0324
0324
0324
0324
0325
0325
0325
0325
0326
0326
0327
0327
0327
0327
0327
0327
0327
0327
0329
0329
0329
0329
0329
0329
0329
0329
0329
0329
0329
0329
033
033
0331
0331
0332
0332
0332
0332
0332
0332
0333
0333
0333
0333
0333
0333
0334
0334
0335
0335
0335
0335
0335
0335
0336
0336
0336
0336
0336
0336
0336
0336
0338
0338
0338
0338
0339
0339
0339
0339
0339
0339
0339
0339
034
034
034
034
0341
0341
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0345
0345
0345
0345
0345
0345
0345
0345
0346
0346
0346
0346
0348
0348
0348
0348
0349
0349
035
035
0351
0351
0351
0351
0351
0351
0351
0351
0352
0352
0352
0352
0352
0352
0354
0354
0355
0355
0356
0356
0357
0357
0358
0358
0358
0358
0358
0358
0359
0359
0361
0361
0361
0361
0361
0361
0361
0361
0361
0361
0361
0361
0364
0364
0364
0364
0364
0364
0367
0367
0368
0368
037
037
0372
0372
0373
0373
0376
0376
0377
0377
038
038
038
038
0383
0383
0386
0386
0389
0389
0389
0389
0395
0395
0398
0398
0404
0404
0405
0405
0405
0405
0412
0412
0422
0422
023
0283
0259
0286
0289
0255
0227
0262
0227
0264
0273
027
0304
0311
0273
0245
027
0289
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0342
0342
0244
0063
0251
0339
0395
0199
0361
0232
0338
037
0318
0282
0235
0233
02
0359
0281
0342
0285
029
0287
0306
021
0219
0284
029
0306
0236
0194
0232
0294
0368
0199
0342
0235
0338
0303
023
0267
038
0203
0346
0364
0278
0176
0214
0281
0312
029
0345
0329
0272
0265
0207
0204
0367
0228
0318
0272
0361
0342
0358
0345
0287
0283
0325
0266
0293
0259
0361
031
0361
0356
0339
0326
0339
0239
0276
0342
0304
032
0285
0225
031
0354
0389
0232
0235
0358
0342
0373
0296
0287
0386
0329
0329
018
0335
0361
0286
0339
0307
032
0334
0372
0405
0335
0345
0321
0364
0335
0316
0332
0338
0338
0345
0342
031
0295
0252
0352
0298
0336
0342
0269
0235
0279
0235
0284
0287
0329
0319
0312
0287
021
0304
0352
0324
0321
0336
037
0303
0287
0373
031
Page 2: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

3

Criteria to be satisfied bull NOVELTY bull DISTINCTNESS bull UNIFORMITY bull STABILITY bull VARIETY DENOMINATION bull FORMALITIES bull PAYMENT OF FEES

ldquoDUSrdquo

CONDITIONS FOR GRANTING A BREEDERrsquoS RIGHT

NO OTHER CONDITIONS

4

IMPORTANCE OF HARMONIZED APPROACH WITHIN UPOV

rArr To facilitate cooperation in DUS testing eg purchase of DUS reports

rArr To establish internationally recognized variety descriptions (effective protection)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

6

Technical Working Party

on Automation

and Computer Programs

(TWC)

COUNCIL

ADMINISTRATIVE AND LEGAL COMMITTEE (CAJ) TECHNICAL COMMITTEE (TC)

CONSULTATIVE COMMITTEE

Technical Working Party

for Vegetables

(TWV)

Technical Working Party

for Ornamental Plants and

Forest Trees

(TWO)

Technical Working Party

for Fruit Crops

(TWF)

Technical Working Party

for Agricultural

Crops

(TWA)

Office of the Union

Working Group on Biochemical and

Molecular Techniques and DNA-Profiling in Particular (BMT)

Document reference Title

TGP15 Guidance on the Use of Biochemical and Molecular Markers in the Examination of Distinctness Uniformity and Stability (DUS)

UPOVINF181 Possible Use of Molecular Markers in the Examination of Distinctness Uniformity and Stability (2011)

Document reference Title

UPOVINF171 Guidelines for DNA Profiling Molecular Marker Selection and Database Construction (ldquoBMT Guidelinesrdquo) (2010)

STATUS OF UPOV DOCUMENTS CONCERNING MOLECULAR TECHNIQUES

Calibrated molecular distances in the management of variety collections

8

02468

10121416182022242628303234363840

0 005 01 015 02 025 03 035 04 045

GA

IumlA D

ista

nces

(Tra

ditio

nal c

hara

cter

istic

di

stan

ces)

Rogers Distances (Molecular marker distances)

GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998

Type 3 Type 1

Type 4Type 2Express - Lady

Duetol - TapidorEurol - Zorro

Ceres - Cheyenne

Express - Zorro

Zorro - Apex

Goeland - Lady

Apex - GoelandApex - Lady

Eurol - Idol

Bristol - Idol

Andol - IdolBristol - Eurol Apex - Express

Bristol - Prestol Andol - Eurol Express - Goeland

Distinctness plus (Traditional characteristics)

Distinctness plus (Molecular markers)

Type 1 Yes Yes Type 2 No No Type 3 Yes No Type 4 No Yes

Graph96

Express-Falcon
Falcon-Lady
Bristol-Falcon
Doublol-Cheyenne
Akamar-Apex
Apex-Goeland
Envol-Idol
Goeland-Lady
Apex-Lady
Bristol-Idol
Eurol-Prestol
Andol-Envol
Bristol-Envol
Idol-Goeland
Apex-Bristol
Apex-Idol
Bristol--Lady
Bristol-Goeland
34 9 varieacuteteacutes preacutesentes
193
759
14 8 varieacuteteacutes preacutesentes
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19951996
23
14
3
14
11
10
20
16
9
24
11
18
21
19
12
22
6
13
6
27
10
32
24
14
20
19
16
23
14
19
19
22
21
14
22
19
13
11
16
21
18
19
11
19
17
19
22
26
25
17
19
21
13
24
14
14
14
9
20
9
11
22
14
21
16
5
10
8
10
16
14
6
16
29
20
14
16
16
23
19
18
3
19
14
4
18
11
16
14
12
19
12
8
13
11
6
16
0
5
1
26
8
19
20
10
20
20
9
14
19
9
4
20
13
15
19
11
16
11
2
11
7
5
16
4
1
5
23
19
18
13
16
16
17
10
11
22
20
18
20
18
13
10
13
23
17
22
24
24
20
25
18
20
18
14
13
23
18
20
18
11
20
10
21
9
11
13
18
10
19
19
17
8
16
14
13
14
20
11
12
14
28
16
24
26
14
19
17
23
20
14
11
16
15
13
10
20
14
3
10
10
17
20
15
14
15
15
19
20
11
19
19
17
14
14
22
16
22
22
14
19
10
19
20
10
20
16
20
20
19
18
25
14
19
12
16
16
17
10
26
8
14
13
11
11
16
12
11
17
8
10
16
19
13
13
18
22
21
12
12
10
15
23
16
26
16
22
14
11
26
18
16
5
8
2
22
16
10
20
11
14
13
13
8
9
15
6
1
11
24
18
15
12
17
17
18
13
9
19
14
12
11
13
19
14
10
18
19
11
24
16
14
21
12
8
14
19
12
26
13
22
21
17
11
24
13
21
19
16
23
17
3
20
18
13
14
21
25
24
12
19
15
22
16
16
23
17
19
15
17
25
21
21
10
13
11
24
14
17
20
24
21
18
13
8
8
18
11
14
12
26
23
3
20
14
20
20
16
19
18
8
11
7
24
13
20
19
16
25
22
8
8
4
15
11
9
8
31
19
13
14
10
24
23
10
12
19
10
6
5
20
14
15
18
14
24
21
9
8
4
16
6
8
5
29
12
10
14
7
26
23
7
11
12
12
14
15
15
16
16
16
16
16
16
16
16
18
20
21
21
21
22
22
22
23
24
25
25
27
6
19
14
0
4
18
11
15
14
12
19
12
6
11
11
6
16
5
1
26
11
18
18
13
17
20
9
13
17
6
5
1
20
12
15
19
8
15
10
1
14
9
8
16
5
8
23
20
20
13
19
16
17
12
6
19
16
1
5
18
14
19
12
14
22
15
11
12
8
5
16
1
8
26
9
16
20
9
20
20
6
27
22
29
26
23
14
28
20
16
19
16
23
24
26
31
29
27
26
23
26
25
26
19
33
19
12
27
10
26
20
8
19
13
16
11
16
12
16
16
18
23
19
12
23
11
20
9
25
24
22
13
16
25
14
32
25
14
19
18
23
24
19
17
26
23
26
15
3
13
10
22
18
20
16
26
24
14
19
18
24
20
24
17
16
20
13
18
26
19
10
13
17
16
12
20
14
14
22
18
13
20
19
22
14
28
9
21
13
14
19
16
10
16
20
14
17
26
22
19
22
17
14
10
7
16
13
19
9
33
13
19
28
25
22
15
20
21
23
20
16
18
19
14
8
21
15
14
17
20
24
26
24
17
16
20
19
16
18
9
25
13
20
19
13
19
20
17
11
17
14
14
17
17
11
18
20
23
23
21
20
17
20
12
25
24
21
22
13
25
16
24
18
9
10
20
23
22
13
11
25
26
13
16
10
7
21
9
12
6
27
14
20
13
15
20
25

Graph97

Apex-Goeland
Eurol-Prestol
Eurol-Andol
Apex-Envol
Apex-Express
Envol-Lady
Akamar-Doublol
Contact-Navajo
Ceres-Goeland
Ceres-Lady
Apex-Ceres
Columbus-Tapidor
Goeland-Lady
Apex-Lady
212
16 8 varieacuteteacutes preacutesentes
16 10 varieacuteteacutes preacutesentes
756
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19961997
18
13
13
18
9
17
24
9
6
4
8
13
15
13
9
10
8
13
10
20
8
15
16
20
6
12
13
18
13
19
14
19
19
15
24
24
20
17
22
17
18
22
17
20
17
17
12
18
18
21
11
20
7
24
13
13
15
18
21
20
18
22
18
18
19
9
3
12
18
15
18
13
9
15
18
9
19
18
18
13
19
13
19
15
19
15
4
12
15
12
16
16
5
14
2
8
8
3
17
6
25
10
16
23
10
15
15
8
18
14
18
19
19
17
15
17
13
22
13
21
16
18
19
21
10
9
11
15
17
16
15
26
14
10
19
9
19
21
15
19
25
26
15
13
9
9
22
23
16
11
16
15
19
17
23
14
21
17
18
11
17
15
17
19
20
4
17
25
10
21
11
15
26
12
10
12
10
11
5
16
5
24
18
17
24
20
18
15
21
24
15
18
12
15
26
10
18
20
22
23
21
13
15
20
13
10
16
12
15
20
13
18
11
17
12
18
9
24
22
15
17
15
21
18
14
12
15
23
22
15
22
17
9
20
20
16
11
18
12
24
5
19
16
6
24
18
12
13
13
11
20
14
7
8
13
14
18
6
11
10
8
8
14
3
16
15
21
12
13
20
4
20
18
16
22
9
15
22
12
7
10
19
18
20
9
10
16
17
15
14
10
18
16
23
10
15
22
8
17
19
16
13
9
26
23
15
8
10
17
23
19
12
22
19
9
14
13
9
23
12
25
15
13
18
13
22
9
5
21
22
12
21
23
13
19
17
11
10
12
11
8
10
1
20
12
16
24
21
19
19
21
15
17
3
14
16
23
10
13
22
14
18
23
11
16
13
12
14
10
8
17
11
2
19
20
20
17
26
13
18
12
2
18
16
12
15
15
18
20
19
10
16
15
15
8
18
11
29
9
18
22
8
18
19
6
9
22
18
8
19
11
10
20
22
6
9
12
12
13
15
13
11
11
7
22
7
16
22
18
19
15
22
10
17
15
8
21
16
11
13
17
11
10
22
11
12
15
13
8
17
11
23
11
13
19
20
16
19
22
8
20
18
3
10
15
5
10
9
10
16
19
8
14
8
11
8
15
9
23
11
15
21
19
12
12
10
13
17
13
17
9
19
16
16
20
8
17
9
10
10
18
11
17
15
8
10
8
11
10
24
19
15
23
10
17
9
6
11
17
5
12
20
8
15
14
1
8
11
7
11
9
8
17
13
17
20
21
15
10
16
20
12
15
25
15
23
24
15
16
14
14
13
20
17
29
22
23
23
10
17
15
17
14
23
19
13
30
8
18
18
10
17
14
18
20
11
3
10
9
12
11
9
7
11
11
8
13
15
6
10
15
15
20
19
15
18
9
16
16
21
17
13
18
16
18
23
16
2
18
16
13
15
11
17
17
6
13
20
18
20
25
16
21
19
23
15
17
24
18
12
15
16
12
24
19
22
22
19
21
10
20
14
10
13
26
9
24
21
20
11
18
10
26
18
20
11
24
21
23
25
21
20
8
18
20
19
24
21
23
15
20
26
26
15
14
6
20
18
15
14
11
18
17
5
12
10
15
19
20
18
19
16
12
19
15
19
15
18
9
26
16
14
12
7
13
15
10
17
15
12
19
13
15
13
19
17
19
15
19
12
15
10
13
20
20
24
15
16
21
13
24
19
8
19
15
21
18
16
20
22
18
21
26
6
22
22
10
23
16
30
19
25
21
14
14
21

Graph98

Type 3
Type 1
Type 4
Type 2
Express - Lady
Duetol - Tapidor
Eurol - Zorro
Ceres - Cheyenne
Express - Zorro
Zorro - Apex
Goeland - Lady
Apex - Goeland
Apex - Lady
Eurol - Idol
Bristol - Idol
Andol - Idol
Bristol - Eurol
Apex - Express
Bristol - Prestol
Andol - Eurol
Express - Goeland
Rogers Distances (Molecular marker distances)
GAIumlA Distances(Traditional characteristic distances)
GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998
24
10
12
28
7
12
16
24
14
19
19
18
18
17
11
20
12
19
17
23
6
28
27
17
17
14
17
24
19
15
15
30
11
13
29
30
20
18
20
15
12
24
25
18
17
15
20
29
22
19
13
20
14
17
10
19
8
8
25
9
10
21
24
26
15
12
5
15
9
20
9
1
11
24
15
17
15
9
24
17
11
12
15
8
10
23
7
6
22
21
24
9
11
8
2
10
24
1
7
1
20
15
19
19
8
12
18
5
28
15
8
10
32
14
10
15
32
27
11
9
2
8
16
29
10
1
7
22
29
5
7
10
12
25
8
7
30
25
23
32
25
28
31
17
18
21
30
27
27
20
34
23
26
25
24
8
29
37
30
17
24
25
12
11
9
7
14
25
4
21
22
18
8
18
11
12
11
19
10
14
10
20
18
24
17
10
18
10
13
16
13
10
6
10
28
4
17
32
19
6
10
7
7
16
25
9
6
6
20
21
16
19
7
14
15
9
24
29
21
22
15
31
21
17
28
19
7
20
21
21
20
30
25
21
23
26
34
22
26
24
11
23
19
14
30
24
21
32
17
22
32
28
16
23
29
24
24
23
32
22
24
23
14
14
30
29
30
16
14
18
19
20
26
24
27
18
18
19
19
16
15
21
22
27
31
30
29
26
28
16
21
24
27
27
10
8
20
19
18
15
9
11
21
8
6
7
23
15
7
9
10
16
24
11
10
8
14
25
16
17
9
3
14
9
18
20
12
11
9
30
18
10
20
29
21
7
7
11
23
23
16
11
12
18
22
10
17
11
13
21
7
18
15
5
8
2
27
11
7
21
24
22
9
7
12
16
22
9
1
7
17
22
8
11
9
11
16
4
17
12
15
2
8
27
12
7
21
24
27
10
11
12
12
27
5
10
1
18
21
13
17
15
8
22
4
11
24
9
10
16
20
11
16
20
23
31
16
23
16
12
16
7
13
9
29
12
25
22
11
21
24
15
20
25
20
24
29
34
19
25
30
32
30
24
23
22
27
16
19
22
22
33
26
35
32
8
32
18
26
12
18
9
1
10
23
10
9
25
22
29
11
16
9
5
7
19
8
0
25
14
21
25
10
14
20
8
19
17
1
7
1
26
14
6
21
24
26
10
11
1
10
13
22
8
8
20
22
12
14
12
16
20
7
17
15
11
1
7
25
10
6
23
23
28
8
12
7
1
9
22
0
8
22
20
19
19
9
11
21
6
23
20
24
20
22
24
20
20
26
14
16
14
18
17
18
29
33
25
20
22
25
22
21
25
13
16
17
6
29
15
15
29
8
18
21
34
14
21
25
22
22
21
12
26
14
22
20
25
26
30
22
23
19
19
28
22
17
19
5
29
24
16
22
30
24
16
10
8
13
25
35
21
12
19
22
26
7
23
14
27
13
27
19
15
19
7
37
17
19
26
29
27
17
17
11
17
22
32
25
14
19
21
30
7
20
16
23
12
17
13
9
8
10
30
10
7
24
30
27
9
11
9
15
11
8
10
12
9
25
22
23
20
22
13
13
17
20
24
12
12
17
18
14
11
16
10
3
13
11
8
21
32
14
16
11
13
23
14
16
22
20
9
14
14
17
18
25
24
10
15
23
14
8
14
21
16
22
24
18
20
20
21
16
19
27
23
13
20
17
17
17
11
5
8
25
13
9
19
18
20
9
7
4
4
15
26
8
7
6
17
19
13
12
13
9
17

Graph99

169
677
61 20 varieacuteteacutes preacutesentes
93 19 varieacuteteacutes preacutesentes
Goeland-Lady
Goeland-Apex
Envol-Idol
Apex-Lady
Ceres-Falcon
Bristol-Eurol
Apex-Envol
Andol-Eurol
Eurol-Idol
Bristol-Prestol
Doublol-Duetol
Capitol-Vivol
Eurol-Samourai
Bristol-Samourai
Akamar-Duetol
Contact-Envol
Duetol-Navajo
Apex-Contact
Prestol-Samouraiet Goeland-Idol
Andol-Zorro
Falcon-Navajo
Andol-Falcon
Falcon-Goeland
Lady-Navajo
Duetol-Lady
Express-Zorro
Contact-Goeland
Duetol-Lady
Distances de ROGERS
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19981999
1
1
1
1
0
0
11
11
1
1
2
2
13
13
5
5
8
8
8
8
6
6
5
5
3
7
3
7
8
10
8
10
8
8
5
5
15
15
15
15
6
6
6
6
5
5
13
13
1
1
14
14
18
18
3
3
2
2
3
3
15
15
12
12
13
13
8
8
12
18
12
18
17
17
20
20
12
12
24
2
24
2
5
20
2
20
5
11
2
11
13
13
1
1
20
20
3
16
3
16
15
15
2
8
8
2
8
8
11
11
12
12
15
15
2
4
4
2
8
13
8
13
17
19
19
17
13
13
11
14
11
14
13
13
12
17
8
12
7
20
20
7
17
8
8
8
11
11
9
9
9
9
12
18
12
18
8
8
15
15
15
15
10
10
5
5
8
8
20
6
6
20
13
13
2
15
15
2
6
6
10
10
16
16
14
6
14
6
15
21
15
21
7
7
21
21
20
7
7
20
15
15
7
7
14
6
6
9
2
2
14
9
10
10
16
16
15
15
13
6
13
6
11
11
19
8
10
10
22
8
22
19
5
5
4
5
4
5
5
7
5
15
5
12
15
12
7
5
0
12
6
0
6
12
22
11
14
14
13
22
11
13
10
6
20
6
20
10
4
15
1
4
1
8
11
15
8
11
18
18
13
15
13
15
13
13
5
17
5
9
3
3
15
12
15
5
12
5
17
9
12
12
14
14
8
10
13
10
13
8
23
6
12
9
6
9
12
23
14
12
10
12
14
10
14
14
16
12
13
12
10
13
10
16
12
15
13
12
9
10
9
5
15
13
2
5
2
13
10
13
4
9
17
9
4
17
10
12
9
10
12
9
16
8
8
12
8
16
12
8
13
13
6
3
6
3
11
12
3
20
12
20
14
11
3
14
18
7
7
18
19
19
13
19
19
11
13
11
3
18
2
20
28
3
20
2
28
12
18
12
5
13
8
12
13
5
8
8
12
8
13
9
13
12
9
16
12
18
18
16
10
10
16
15
16
15
14
11
14
11
10
16
8
16
10
8
10
2
8
12
2
6
11
23
10
6
19
8
11
23
12
19
6
6
10
7
8
1
9
21
9
21
7
1
10
8
18
14
18
14
17
17
8
8
9
5
14
3
9
21
5
16
13
14
16
13
21
3
10
5
5
10
13
13
4
15
4
15
5
8
8
8
5
8
5
5
17
15
17
15
10
8
7
8
10
9
20
20
7
9
15
12
10
15
18
12
18
10
15
23
15
23
12
12
19
19
14
14
14
11
11
8
14
8
17
6
17
6
13
13
10
2
2
5
18
10
5
18
10
7
10
13
15
13
15
15
13
15
13
7
2
2
8
8
14
7
10
14
7
10
11
14
11
18
14
18
13
13
16
16
15
16
16
15
9
2
11
2
9
12
12
11
12
15
12
15
8
11
12
14
14
8
12
11
11
24
11
24
13
13
13
15
16
16
22
13
15
16
22
13
15
15
16
14
13
12
14
12
10
10
15
10
6
10
15
6
9
9
13
13
10
11
11
10
14
14
10
10
17
8
7
8
8
17
8
7
8
8
12
2
2
12
17
17
12
12
18
18
18
18
5
8
5
16
16
8
14
14
14
8
22
22
13
13
14
14
9
14
9
8
13
13
11
13
11
13
9
9
12
12
9
9
13
13
10
10
13
13
6
6
11
11
17
17
9
9
15
15
9
9
6
6
12
12
24
24
8
8
12
19
12
19
9
9
12
12

Model 1 Characteristic-specific molecular markers

Example 1 gene specific marker for herbicide tolerance introduced by genetic modification On the basis that [hellip] - there is verification of the reliability of the link between the marker and the characteristic - different markers for the same characteristic are different methods for examining the same characteristic [hellip]

Fusarium race 0 ex 1 Example Characteristic-specific molecular marker with incomplete information on state of expression

Model 1 Characteristic-specific molecular markers

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

ex Maize parent lines

Model 2 Combining phenotypic and molecular distances in the management of variety collections

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

2

02

Model 2 Combining phenotypic and molecular distances in the management of variety collections (continued)

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

13

New Model 3 Genetic selection of similar varieties for the first growing cycle

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
0038
0038
0042
0042
0045
0045
0063
0063
0067
0067
0068
0068
0075
0075
0106
0106
011
011
0114
0114
0115
0115
0116
0116
0123
0123
0123
0123
0131
0131
0131
0131
0132
0132
0145
0145
0149
0149
0149
0149
017
017
0176
0176
018
018
0185
0185
0186
0186
0194
0194
0198
0198
0199
0199
02
02
0201
0201
0202
0202
0203
0203
0204
0204
0207
0207
021
021
021
021
0213
0213
0214
0214
0217
0217
0217
0217
0217
0217
0219
0219
0219
0219
0219
0219
0219
0219
022
022
0224
0224
0225
0225
0225
0225
0225
0225
0226
0226
0227
0227
0227
0227
0227
0227
0228
0228
0228
0228
0228
0228
023
023
023
023
023
023
023
023
0231
0231
0231
0231
0232
0232
0232
0232
0232
0232
0233
0233
0235
0235
0235
0235
0235
0235
0235
0235
0235
0235
0236
0236
0236
0236
0238
0238
0238
0238
0239
0239
0239
0239
0241
0241
0242
0242
0244
0244
0245
0245
0246
0246
0247
0247
0249
0249
0249
0249
025
025
0251
0251
0251
0251
0252
0252
0252
0252
0252
0252
0253
0253
0253
0253
0254
0254
0254
0254
0255
0255
0259
0259
0259
0259
0259
0259
026
026
026
026
0262
0262
0262
0262
0262
0262
0262
0262
0264
0264
0265
0265
0266
0266
0267
0267
0267
0267
0268
0268
0269
0269
0269
0269
0269
0269
0269
0269
027
027
027
027
027
027
0272
0272
0272
0272
0272
0272
0272
0272
0272
0272
0273
0273
0273
0273
0273
0273
0275
0275
0275
0275
0275
0275
0275
0275
0276
0276
0276
0276
0276
0276
0278
0278
0278
0278
0278
0278
0278
0278
0278
0278
0279
0279
0279
0279
0279
0279
028
028
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0282
0282
0282
0282
0283
0283
0283
0283
0283
0283
0284
0284
0284
0284
0284
0284
0284
0284
0285
0285
0285
0285
0285
0285
0285
0285
0286
0286
0286
0286
0286
0286
0286
0286
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0288
0288
0288
0288
0288
0288
0289
0289
0289
0289
0289
0289
029
029
029
029
029
029
029
029
0291
0291
0292
0292
0292
0292
0293
0293
0293
0293
0293
0293
0293
0293
0293
0293
0294
0294
0294
0294
0294
0294
0295
0295
0295
0295
0295
0295
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0298
0298
0298
0298
0298
0298
0298
0298
0298
0298
0299
0299
0299
0299
0299
0299
0299
0299
0299
0299
03
03
0302
0302
0302
0302
0303
0303
0303
0303
0304
0304
0304
0304
0304
0304
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0307
0307
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
031
031
031
031
031
031
0311
0311
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0313
0313
0313
0313
0314
0314
0315
0315
0315
0315
0316
0316
0316
0316
0316
0316
0318
0318
0318
0318
0318
0318
0319
0319
0319
0319
0319
0319
0319
0319
0319
0319
032
032
032
032
032
032
032
032
0321
0321
0321
0321
0322
0322
0322
0322
0323
0323
0324
0324
0324
0324
0324
0324
0325
0325
0325
0325
0326
0326
0327
0327
0327
0327
0327
0327
0327
0327
0329
0329
0329
0329
0329
0329
0329
0329
0329
0329
0329
0329
033
033
0331
0331
0332
0332
0332
0332
0332
0332
0333
0333
0333
0333
0333
0333
0334
0334
0335
0335
0335
0335
0335
0335
0336
0336
0336
0336
0336
0336
0336
0336
0338
0338
0338
0338
0339
0339
0339
0339
0339
0339
0339
0339
034
034
034
034
0341
0341
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0345
0345
0345
0345
0345
0345
0345
0345
0346
0346
0346
0346
0348
0348
0348
0348
0349
0349
035
035
0351
0351
0351
0351
0351
0351
0351
0351
0352
0352
0352
0352
0352
0352
0354
0354
0355
0355
0356
0356
0357
0357
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023
0276
0253
0296
0219
0045
0225
0299
0327
0251
0232
0228
031
0335
0324
0295
0252
0333
0327
0306
0275
0275
0302
0254
0302
0185
0231
0288
0322
0398
0269
0296
0319
0315
0299
0361
0339
0294
0318
0354
0316
0321
0292
0342
0404
0298
0383
0351
0327
033
0331
0306
0312
0309
0377
0412
0389
0358
0299
0313
038
0327
0361
0395
0368
0272
0389
0332
0336
0312
0312
0132
0242
0116
0364
0357
0376
034
0342
0323
032
0075
0115
0282
0342
0342
0244
0063
0251
0339
0395
0199
0361
0232
0338
037
0318
0282
0235
0233
02
0359
0281
0342
0285
029
0287
0306
021
0219
0284
029
0306
0236
0194
0232
0294
0368
0199
0342
0235
0338
0303
023
0267
038
0203
0346
0364
0278
0176
0214
0281
0312
029
0345
0329
0272
0265
0207
0204
0367
0228
0318
0272
0361
0342
0358
0345
0287
0283
0325
0266
0293
0259
0361
031
0361
0356
0339
0326
0339
0239
0276
0342
0304
032
0285
0225
031
0354
0389
0232
0235
0358
0342
0373
0296
0287
0386
0329
0329
018
0335
0361
0286
0339
0307
032
0334
0372
0405
0335
0345
0321
0364
0335
0316
0332
0338
0338
0345
0342
031
0295
0252
0352
0298
0336
0342
0269
0235
0279
0235
0284
0287
0329
0319
0312
0287
021
0304
0352
0324
0321
0336
037
0303
0287
0373
031
Page 3: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

3

Criteria to be satisfied bull NOVELTY bull DISTINCTNESS bull UNIFORMITY bull STABILITY bull VARIETY DENOMINATION bull FORMALITIES bull PAYMENT OF FEES

ldquoDUSrdquo

CONDITIONS FOR GRANTING A BREEDERrsquoS RIGHT

NO OTHER CONDITIONS

4

IMPORTANCE OF HARMONIZED APPROACH WITHIN UPOV

rArr To facilitate cooperation in DUS testing eg purchase of DUS reports

rArr To establish internationally recognized variety descriptions (effective protection)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

6

Technical Working Party

on Automation

and Computer Programs

(TWC)

COUNCIL

ADMINISTRATIVE AND LEGAL COMMITTEE (CAJ) TECHNICAL COMMITTEE (TC)

CONSULTATIVE COMMITTEE

Technical Working Party

for Vegetables

(TWV)

Technical Working Party

for Ornamental Plants and

Forest Trees

(TWO)

Technical Working Party

for Fruit Crops

(TWF)

Technical Working Party

for Agricultural

Crops

(TWA)

Office of the Union

Working Group on Biochemical and

Molecular Techniques and DNA-Profiling in Particular (BMT)

Document reference Title

TGP15 Guidance on the Use of Biochemical and Molecular Markers in the Examination of Distinctness Uniformity and Stability (DUS)

UPOVINF181 Possible Use of Molecular Markers in the Examination of Distinctness Uniformity and Stability (2011)

Document reference Title

UPOVINF171 Guidelines for DNA Profiling Molecular Marker Selection and Database Construction (ldquoBMT Guidelinesrdquo) (2010)

STATUS OF UPOV DOCUMENTS CONCERNING MOLECULAR TECHNIQUES

Calibrated molecular distances in the management of variety collections

8

02468

10121416182022242628303234363840

0 005 01 015 02 025 03 035 04 045

GA

IumlA D

ista

nces

(Tra

ditio

nal c

hara

cter

istic

di

stan

ces)

Rogers Distances (Molecular marker distances)

GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998

Type 3 Type 1

Type 4Type 2Express - Lady

Duetol - TapidorEurol - Zorro

Ceres - Cheyenne

Express - Zorro

Zorro - Apex

Goeland - Lady

Apex - GoelandApex - Lady

Eurol - Idol

Bristol - Idol

Andol - IdolBristol - Eurol Apex - Express

Bristol - Prestol Andol - Eurol Express - Goeland

Distinctness plus (Traditional characteristics)

Distinctness plus (Molecular markers)

Type 1 Yes Yes Type 2 No No Type 3 Yes No Type 4 No Yes

Graph96

Express-Falcon
Falcon-Lady
Bristol-Falcon
Doublol-Cheyenne
Akamar-Apex
Apex-Goeland
Envol-Idol
Goeland-Lady
Apex-Lady
Bristol-Idol
Eurol-Prestol
Andol-Envol
Bristol-Envol
Idol-Goeland
Apex-Bristol
Apex-Idol
Bristol--Lady
Bristol-Goeland
34 9 varieacuteteacutes preacutesentes
193
759
14 8 varieacuteteacutes preacutesentes
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19951996
23
14
3
14
11
10
20
16
9
24
11
18
21
19
12
22
6
13
6
27
10
32
24
14
20
19
16
23
14
19
19
22
21
14
22
19
13
11
16
21
18
19
11
19
17
19
22
26
25
17
19
21
13
24
14
14
14
9
20
9
11
22
14
21
16
5
10
8
10
16
14
6
16
29
20
14
16
16
23
19
18
3
19
14
4
18
11
16
14
12
19
12
8
13
11
6
16
0
5
1
26
8
19
20
10
20
20
9
14
19
9
4
20
13
15
19
11
16
11
2
11
7
5
16
4
1
5
23
19
18
13
16
16
17
10
11
22
20
18
20
18
13
10
13
23
17
22
24
24
20
25
18
20
18
14
13
23
18
20
18
11
20
10
21
9
11
13
18
10
19
19
17
8
16
14
13
14
20
11
12
14
28
16
24
26
14
19
17
23
20
14
11
16
15
13
10
20
14
3
10
10
17
20
15
14
15
15
19
20
11
19
19
17
14
14
22
16
22
22
14
19
10
19
20
10
20
16
20
20
19
18
25
14
19
12
16
16
17
10
26
8
14
13
11
11
16
12
11
17
8
10
16
19
13
13
18
22
21
12
12
10
15
23
16
26
16
22
14
11
26
18
16
5
8
2
22
16
10
20
11
14
13
13
8
9
15
6
1
11
24
18
15
12
17
17
18
13
9
19
14
12
11
13
19
14
10
18
19
11
24
16
14
21
12
8
14
19
12
26
13
22
21
17
11
24
13
21
19
16
23
17
3
20
18
13
14
21
25
24
12
19
15
22
16
16
23
17
19
15
17
25
21
21
10
13
11
24
14
17
20
24
21
18
13
8
8
18
11
14
12
26
23
3
20
14
20
20
16
19
18
8
11
7
24
13
20
19
16
25
22
8
8
4
15
11
9
8
31
19
13
14
10
24
23
10
12
19
10
6
5
20
14
15
18
14
24
21
9
8
4
16
6
8
5
29
12
10
14
7
26
23
7
11
12
12
14
15
15
16
16
16
16
16
16
16
16
18
20
21
21
21
22
22
22
23
24
25
25
27
6
19
14
0
4
18
11
15
14
12
19
12
6
11
11
6
16
5
1
26
11
18
18
13
17
20
9
13
17
6
5
1
20
12
15
19
8
15
10
1
14
9
8
16
5
8
23
20
20
13
19
16
17
12
6
19
16
1
5
18
14
19
12
14
22
15
11
12
8
5
16
1
8
26
9
16
20
9
20
20
6
27
22
29
26
23
14
28
20
16
19
16
23
24
26
31
29
27
26
23
26
25
26
19
33
19
12
27
10
26
20
8
19
13
16
11
16
12
16
16
18
23
19
12
23
11
20
9
25
24
22
13
16
25
14
32
25
14
19
18
23
24
19
17
26
23
26
15
3
13
10
22
18
20
16
26
24
14
19
18
24
20
24
17
16
20
13
18
26
19
10
13
17
16
12
20
14
14
22
18
13
20
19
22
14
28
9
21
13
14
19
16
10
16
20
14
17
26
22
19
22
17
14
10
7
16
13
19
9
33
13
19
28
25
22
15
20
21
23
20
16
18
19
14
8
21
15
14
17
20
24
26
24
17
16
20
19
16
18
9
25
13
20
19
13
19
20
17
11
17
14
14
17
17
11
18
20
23
23
21
20
17
20
12
25
24
21
22
13
25
16
24
18
9
10
20
23
22
13
11
25
26
13
16
10
7
21
9
12
6
27
14
20
13
15
20
25

Graph97

Apex-Goeland
Eurol-Prestol
Eurol-Andol
Apex-Envol
Apex-Express
Envol-Lady
Akamar-Doublol
Contact-Navajo
Ceres-Goeland
Ceres-Lady
Apex-Ceres
Columbus-Tapidor
Goeland-Lady
Apex-Lady
212
16 8 varieacuteteacutes preacutesentes
16 10 varieacuteteacutes preacutesentes
756
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19961997
18
13
13
18
9
17
24
9
6
4
8
13
15
13
9
10
8
13
10
20
8
15
16
20
6
12
13
18
13
19
14
19
19
15
24
24
20
17
22
17
18
22
17
20
17
17
12
18
18
21
11
20
7
24
13
13
15
18
21
20
18
22
18
18
19
9
3
12
18
15
18
13
9
15
18
9
19
18
18
13
19
13
19
15
19
15
4
12
15
12
16
16
5
14
2
8
8
3
17
6
25
10
16
23
10
15
15
8
18
14
18
19
19
17
15
17
13
22
13
21
16
18
19
21
10
9
11
15
17
16
15
26
14
10
19
9
19
21
15
19
25
26
15
13
9
9
22
23
16
11
16
15
19
17
23
14
21
17
18
11
17
15
17
19
20
4
17
25
10
21
11
15
26
12
10
12
10
11
5
16
5
24
18
17
24
20
18
15
21
24
15
18
12
15
26
10
18
20
22
23
21
13
15
20
13
10
16
12
15
20
13
18
11
17
12
18
9
24
22
15
17
15
21
18
14
12
15
23
22
15
22
17
9
20
20
16
11
18
12
24
5
19
16
6
24
18
12
13
13
11
20
14
7
8
13
14
18
6
11
10
8
8
14
3
16
15
21
12
13
20
4
20
18
16
22
9
15
22
12
7
10
19
18
20
9
10
16
17
15
14
10
18
16
23
10
15
22
8
17
19
16
13
9
26
23
15
8
10
17
23
19
12
22
19
9
14
13
9
23
12
25
15
13
18
13
22
9
5
21
22
12
21
23
13
19
17
11
10
12
11
8
10
1
20
12
16
24
21
19
19
21
15
17
3
14
16
23
10
13
22
14
18
23
11
16
13
12
14
10
8
17
11
2
19
20
20
17
26
13
18
12
2
18
16
12
15
15
18
20
19
10
16
15
15
8
18
11
29
9
18
22
8
18
19
6
9
22
18
8
19
11
10
20
22
6
9
12
12
13
15
13
11
11
7
22
7
16
22
18
19
15
22
10
17
15
8
21
16
11
13
17
11
10
22
11
12
15
13
8
17
11
23
11
13
19
20
16
19
22
8
20
18
3
10
15
5
10
9
10
16
19
8
14
8
11
8
15
9
23
11
15
21
19
12
12
10
13
17
13
17
9
19
16
16
20
8
17
9
10
10
18
11
17
15
8
10
8
11
10
24
19
15
23
10
17
9
6
11
17
5
12
20
8
15
14
1
8
11
7
11
9
8
17
13
17
20
21
15
10
16
20
12
15
25
15
23
24
15
16
14
14
13
20
17
29
22
23
23
10
17
15
17
14
23
19
13
30
8
18
18
10
17
14
18
20
11
3
10
9
12
11
9
7
11
11
8
13
15
6
10
15
15
20
19
15
18
9
16
16
21
17
13
18
16
18
23
16
2
18
16
13
15
11
17
17
6
13
20
18
20
25
16
21
19
23
15
17
24
18
12
15
16
12
24
19
22
22
19
21
10
20
14
10
13
26
9
24
21
20
11
18
10
26
18
20
11
24
21
23
25
21
20
8
18
20
19
24
21
23
15
20
26
26
15
14
6
20
18
15
14
11
18
17
5
12
10
15
19
20
18
19
16
12
19
15
19
15
18
9
26
16
14
12
7
13
15
10
17
15
12
19
13
15
13
19
17
19
15
19
12
15
10
13
20
20
24
15
16
21
13
24
19
8
19
15
21
18
16
20
22
18
21
26
6
22
22
10
23
16
30
19
25
21
14
14
21

Graph98

Type 3
Type 1
Type 4
Type 2
Express - Lady
Duetol - Tapidor
Eurol - Zorro
Ceres - Cheyenne
Express - Zorro
Zorro - Apex
Goeland - Lady
Apex - Goeland
Apex - Lady
Eurol - Idol
Bristol - Idol
Andol - Idol
Bristol - Eurol
Apex - Express
Bristol - Prestol
Andol - Eurol
Express - Goeland
Rogers Distances (Molecular marker distances)
GAIumlA Distances(Traditional characteristic distances)
GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998
24
10
12
28
7
12
16
24
14
19
19
18
18
17
11
20
12
19
17
23
6
28
27
17
17
14
17
24
19
15
15
30
11
13
29
30
20
18
20
15
12
24
25
18
17
15
20
29
22
19
13
20
14
17
10
19
8
8
25
9
10
21
24
26
15
12
5
15
9
20
9
1
11
24
15
17
15
9
24
17
11
12
15
8
10
23
7
6
22
21
24
9
11
8
2
10
24
1
7
1
20
15
19
19
8
12
18
5
28
15
8
10
32
14
10
15
32
27
11
9
2
8
16
29
10
1
7
22
29
5
7
10
12
25
8
7
30
25
23
32
25
28
31
17
18
21
30
27
27
20
34
23
26
25
24
8
29
37
30
17
24
25
12
11
9
7
14
25
4
21
22
18
8
18
11
12
11
19
10
14
10
20
18
24
17
10
18
10
13
16
13
10
6
10
28
4
17
32
19
6
10
7
7
16
25
9
6
6
20
21
16
19
7
14
15
9
24
29
21
22
15
31
21
17
28
19
7
20
21
21
20
30
25
21
23
26
34
22
26
24
11
23
19
14
30
24
21
32
17
22
32
28
16
23
29
24
24
23
32
22
24
23
14
14
30
29
30
16
14
18
19
20
26
24
27
18
18
19
19
16
15
21
22
27
31
30
29
26
28
16
21
24
27
27
10
8
20
19
18
15
9
11
21
8
6
7
23
15
7
9
10
16
24
11
10
8
14
25
16
17
9
3
14
9
18
20
12
11
9
30
18
10
20
29
21
7
7
11
23
23
16
11
12
18
22
10
17
11
13
21
7
18
15
5
8
2
27
11
7
21
24
22
9
7
12
16
22
9
1
7
17
22
8
11
9
11
16
4
17
12
15
2
8
27
12
7
21
24
27
10
11
12
12
27
5
10
1
18
21
13
17
15
8
22
4
11
24
9
10
16
20
11
16
20
23
31
16
23
16
12
16
7
13
9
29
12
25
22
11
21
24
15
20
25
20
24
29
34
19
25
30
32
30
24
23
22
27
16
19
22
22
33
26
35
32
8
32
18
26
12
18
9
1
10
23
10
9
25
22
29
11
16
9
5
7
19
8
0
25
14
21
25
10
14
20
8
19
17
1
7
1
26
14
6
21
24
26
10
11
1
10
13
22
8
8
20
22
12
14
12
16
20
7
17
15
11
1
7
25
10
6
23
23
28
8
12
7
1
9
22
0
8
22
20
19
19
9
11
21
6
23
20
24
20
22
24
20
20
26
14
16
14
18
17
18
29
33
25
20
22
25
22
21
25
13
16
17
6
29
15
15
29
8
18
21
34
14
21
25
22
22
21
12
26
14
22
20
25
26
30
22
23
19
19
28
22
17
19
5
29
24
16
22
30
24
16
10
8
13
25
35
21
12
19
22
26
7
23
14
27
13
27
19
15
19
7
37
17
19
26
29
27
17
17
11
17
22
32
25
14
19
21
30
7
20
16
23
12
17
13
9
8
10
30
10
7
24
30
27
9
11
9
15
11
8
10
12
9
25
22
23
20
22
13
13
17
20
24
12
12
17
18
14
11
16
10
3
13
11
8
21
32
14
16
11
13
23
14
16
22
20
9
14
14
17
18
25
24
10
15
23
14
8
14
21
16
22
24
18
20
20
21
16
19
27
23
13
20
17
17
17
11
5
8
25
13
9
19
18
20
9
7
4
4
15
26
8
7
6
17
19
13
12
13
9
17

Graph99

169
677
61 20 varieacuteteacutes preacutesentes
93 19 varieacuteteacutes preacutesentes
Goeland-Lady
Goeland-Apex
Envol-Idol
Apex-Lady
Ceres-Falcon
Bristol-Eurol
Apex-Envol
Andol-Eurol
Eurol-Idol
Bristol-Prestol
Doublol-Duetol
Capitol-Vivol
Eurol-Samourai
Bristol-Samourai
Akamar-Duetol
Contact-Envol
Duetol-Navajo
Apex-Contact
Prestol-Samouraiet Goeland-Idol
Andol-Zorro
Falcon-Navajo
Andol-Falcon
Falcon-Goeland
Lady-Navajo
Duetol-Lady
Express-Zorro
Contact-Goeland
Duetol-Lady
Distances de ROGERS
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19981999
1
1
1
1
0
0
11
11
1
1
2
2
13
13
5
5
8
8
8
8
6
6
5
5
3
7
3
7
8
10
8
10
8
8
5
5
15
15
15
15
6
6
6
6
5
5
13
13
1
1
14
14
18
18
3
3
2
2
3
3
15
15
12
12
13
13
8
8
12
18
12
18
17
17
20
20
12
12
24
2
24
2
5
20
2
20
5
11
2
11
13
13
1
1
20
20
3
16
3
16
15
15
2
8
8
2
8
8
11
11
12
12
15
15
2
4
4
2
8
13
8
13
17
19
19
17
13
13
11
14
11
14
13
13
12
17
8
12
7
20
20
7
17
8
8
8
11
11
9
9
9
9
12
18
12
18
8
8
15
15
15
15
10
10
5
5
8
8
20
6
6
20
13
13
2
15
15
2
6
6
10
10
16
16
14
6
14
6
15
21
15
21
7
7
21
21
20
7
7
20
15
15
7
7
14
6
6
9
2
2
14
9
10
10
16
16
15
15
13
6
13
6
11
11
19
8
10
10
22
8
22
19
5
5
4
5
4
5
5
7
5
15
5
12
15
12
7
5
0
12
6
0
6
12
22
11
14
14
13
22
11
13
10
6
20
6
20
10
4
15
1
4
1
8
11
15
8
11
18
18
13
15
13
15
13
13
5
17
5
9
3
3
15
12
15
5
12
5
17
9
12
12
14
14
8
10
13
10
13
8
23
6
12
9
6
9
12
23
14
12
10
12
14
10
14
14
16
12
13
12
10
13
10
16
12
15
13
12
9
10
9
5
15
13
2
5
2
13
10
13
4
9
17
9
4
17
10
12
9
10
12
9
16
8
8
12
8
16
12
8
13
13
6
3
6
3
11
12
3
20
12
20
14
11
3
14
18
7
7
18
19
19
13
19
19
11
13
11
3
18
2
20
28
3
20
2
28
12
18
12
5
13
8
12
13
5
8
8
12
8
13
9
13
12
9
16
12
18
18
16
10
10
16
15
16
15
14
11
14
11
10
16
8
16
10
8
10
2
8
12
2
6
11
23
10
6
19
8
11
23
12
19
6
6
10
7
8
1
9
21
9
21
7
1
10
8
18
14
18
14
17
17
8
8
9
5
14
3
9
21
5
16
13
14
16
13
21
3
10
5
5
10
13
13
4
15
4
15
5
8
8
8
5
8
5
5
17
15
17
15
10
8
7
8
10
9
20
20
7
9
15
12
10
15
18
12
18
10
15
23
15
23
12
12
19
19
14
14
14
11
11
8
14
8
17
6
17
6
13
13
10
2
2
5
18
10
5
18
10
7
10
13
15
13
15
15
13
15
13
7
2
2
8
8
14
7
10
14
7
10
11
14
11
18
14
18
13
13
16
16
15
16
16
15
9
2
11
2
9
12
12
11
12
15
12
15
8
11
12
14
14
8
12
11
11
24
11
24
13
13
13
15
16
16
22
13
15
16
22
13
15
15
16
14
13
12
14
12
10
10
15
10
6
10
15
6
9
9
13
13
10
11
11
10
14
14
10
10
17
8
7
8
8
17
8
7
8
8
12
2
2
12
17
17
12
12
18
18
18
18
5
8
5
16
16
8
14
14
14
8
22
22
13
13
14
14
9
14
9
8
13
13
11
13
11
13
9
9
12
12
9
9
13
13
10
10
13
13
6
6
11
11
17
17
9
9
15
15
9
9
6
6
12
12
24
24
8
8
12
19
12
19
9
9
12
12

Model 1 Characteristic-specific molecular markers

Example 1 gene specific marker for herbicide tolerance introduced by genetic modification On the basis that [hellip] - there is verification of the reliability of the link between the marker and the characteristic - different markers for the same characteristic are different methods for examining the same characteristic [hellip]

Fusarium race 0 ex 1 Example Characteristic-specific molecular marker with incomplete information on state of expression

Model 1 Characteristic-specific molecular markers

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

ex Maize parent lines

Model 2 Combining phenotypic and molecular distances in the management of variety collections

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

2

02

Model 2 Combining phenotypic and molecular distances in the management of variety collections (continued)

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

13

New Model 3 Genetic selection of similar varieties for the first growing cycle

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
0038
0038
0042
0042
0045
0045
0063
0063
0067
0067
0068
0068
0075
0075
0106
0106
011
011
0114
0114
0115
0115
0116
0116
0123
0123
0123
0123
0131
0131
0131
0131
0132
0132
0145
0145
0149
0149
0149
0149
017
017
0176
0176
018
018
0185
0185
0186
0186
0194
0194
0198
0198
0199
0199
02
02
0201
0201
0202
0202
0203
0203
0204
0204
0207
0207
021
021
021
021
0213
0213
0214
0214
0217
0217
0217
0217
0217
0217
0219
0219
0219
0219
0219
0219
0219
0219
022
022
0224
0224
0225
0225
0225
0225
0225
0225
0226
0226
0227
0227
0227
0227
0227
0227
0228
0228
0228
0228
0228
0228
023
023
023
023
023
023
023
023
0231
0231
0231
0231
0232
0232
0232
0232
0232
0232
0233
0233
0235
0235
0235
0235
0235
0235
0235
0235
0235
0235
0236
0236
0236
0236
0238
0238
0238
0238
0239
0239
0239
0239
0241
0241
0242
0242
0244
0244
0245
0245
0246
0246
0247
0247
0249
0249
0249
0249
025
025
0251
0251
0251
0251
0252
0252
0252
0252
0252
0252
0253
0253
0253
0253
0254
0254
0254
0254
0255
0255
0259
0259
0259
0259
0259
0259
026
026
026
026
0262
0262
0262
0262
0262
0262
0262
0262
0264
0264
0265
0265
0266
0266
0267
0267
0267
0267
0268
0268
0269
0269
0269
0269
0269
0269
0269
0269
027
027
027
027
027
027
0272
0272
0272
0272
0272
0272
0272
0272
0272
0272
0273
0273
0273
0273
0273
0273
0275
0275
0275
0275
0275
0275
0275
0275
0276
0276
0276
0276
0276
0276
0278
0278
0278
0278
0278
0278
0278
0278
0278
0278
0279
0279
0279
0279
0279
0279
028
028
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0282
0282
0282
0282
0283
0283
0283
0283
0283
0283
0284
0284
0284
0284
0284
0284
0284
0284
0285
0285
0285
0285
0285
0285
0285
0285
0286
0286
0286
0286
0286
0286
0286
0286
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0288
0288
0288
0288
0288
0288
0289
0289
0289
0289
0289
0289
029
029
029
029
029
029
029
029
0291
0291
0292
0292
0292
0292
0293
0293
0293
0293
0293
0293
0293
0293
0293
0293
0294
0294
0294
0294
0294
0294
0295
0295
0295
0295
0295
0295
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0298
0298
0298
0298
0298
0298
0298
0298
0298
0298
0299
0299
0299
0299
0299
0299
0299
0299
0299
0299
03
03
0302
0302
0302
0302
0303
0303
0303
0303
0304
0304
0304
0304
0304
0304
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0307
0307
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
031
031
031
031
031
031
0311
0311
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0313
0313
0313
0313
0314
0314
0315
0315
0315
0315
0316
0316
0316
0316
0316
0316
0318
0318
0318
0318
0318
0318
0319
0319
0319
0319
0319
0319
0319
0319
0319
0319
032
032
032
032
032
032
032
032
0321
0321
0321
0321
0322
0322
0322
0322
0323
0323
0324
0324
0324
0324
0324
0324
0325
0325
0325
0325
0326
0326
0327
0327
0327
0327
0327
0327
0327
0327
0329
0329
0329
0329
0329
0329
0329
0329
0329
0329
0329
0329
033
033
0331
0331
0332
0332
0332
0332
0332
0332
0333
0333
0333
0333
0333
0333
0334
0334
0335
0335
0335
0335
0335
0335
0336
0336
0336
0336
0336
0336
0336
0336
0338
0338
0338
0338
0339
0339
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0299
0342
0306
0207
032
0345
021
027
028
0272
0038
0227
0358
0281
0236
0202
0246
0275
0288
0217
0249
023
0262
0198
0201
0045
0319
0313
0244
0236
0204
0285
0321
0304
0289
0289
0123
0219
0114
0348
0306
0346
0294
0296
029
0306
0042
0106
0281
0287
0296
0201
0225
0315
038
0063
0194
0367
0225
0364
0352
0292
0298
0287
0067
0247
0351
0309
0269
0232
0281
0299
0315
023
0276
0253
0296
0219
0045
0225
0299
0327
0251
0232
0228
031
0335
0324
0295
0252
0333
0327
0306
0275
0275
0302
0254
0302
0185
0231
0288
0322
0398
0269
0296
0319
0315
0299
0361
0339
0294
0318
0354
0316
0321
0292
0342
0404
0298
0383
0351
0327
033
0331
0306
0312
0309
0377
0412
0389
0358
0299
0313
038
0327
0361
0395
0368
0272
0389
0332
0336
0312
0312
0132
0242
0116
0364
0357
0376
034
0342
0323
032
0075
0115
0282
0342
0342
0244
0063
0251
0339
0395
0199
0361
0232
0338
037
0318
0282
0235
0233
02
0359
0281
0342
0285
029
0287
0306
021
0219
0284
029
0306
0236
0194
0232
0294
0368
0199
0342
0235
0338
0303
023
0267
038
0203
0346
0364
0278
0176
0214
0281
0312
029
0345
0329
0272
0265
0207
0204
0367
0228
0318
0272
0361
0342
0358
0345
0287
0283
0325
0266
0293
0259
0361
031
0361
0356
0339
0326
0339
0239
0276
0342
0304
032
0285
0225
031
0354
0389
0232
0235
0358
0342
0373
0296
0287
0386
0329
0329
018
0335
0361
0286
0339
0307
032
0334
0372
0405
0335
0345
0321
0364
0335
0316
0332
0338
0338
0345
0342
031
0295
0252
0352
0298
0336
0342
0269
0235
0279
0235
0284
0287
0329
0319
0312
0287
021
0304
0352
0324
0321
0336
037
0303
0287
0373
031
Page 4: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

4

IMPORTANCE OF HARMONIZED APPROACH WITHIN UPOV

rArr To facilitate cooperation in DUS testing eg purchase of DUS reports

rArr To establish internationally recognized variety descriptions (effective protection)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

6

Technical Working Party

on Automation

and Computer Programs

(TWC)

COUNCIL

ADMINISTRATIVE AND LEGAL COMMITTEE (CAJ) TECHNICAL COMMITTEE (TC)

CONSULTATIVE COMMITTEE

Technical Working Party

for Vegetables

(TWV)

Technical Working Party

for Ornamental Plants and

Forest Trees

(TWO)

Technical Working Party

for Fruit Crops

(TWF)

Technical Working Party

for Agricultural

Crops

(TWA)

Office of the Union

Working Group on Biochemical and

Molecular Techniques and DNA-Profiling in Particular (BMT)

Document reference Title

TGP15 Guidance on the Use of Biochemical and Molecular Markers in the Examination of Distinctness Uniformity and Stability (DUS)

UPOVINF181 Possible Use of Molecular Markers in the Examination of Distinctness Uniformity and Stability (2011)

Document reference Title

UPOVINF171 Guidelines for DNA Profiling Molecular Marker Selection and Database Construction (ldquoBMT Guidelinesrdquo) (2010)

STATUS OF UPOV DOCUMENTS CONCERNING MOLECULAR TECHNIQUES

Calibrated molecular distances in the management of variety collections

8

02468

10121416182022242628303234363840

0 005 01 015 02 025 03 035 04 045

GA

IumlA D

ista

nces

(Tra

ditio

nal c

hara

cter

istic

di

stan

ces)

Rogers Distances (Molecular marker distances)

GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998

Type 3 Type 1

Type 4Type 2Express - Lady

Duetol - TapidorEurol - Zorro

Ceres - Cheyenne

Express - Zorro

Zorro - Apex

Goeland - Lady

Apex - GoelandApex - Lady

Eurol - Idol

Bristol - Idol

Andol - IdolBristol - Eurol Apex - Express

Bristol - Prestol Andol - Eurol Express - Goeland

Distinctness plus (Traditional characteristics)

Distinctness plus (Molecular markers)

Type 1 Yes Yes Type 2 No No Type 3 Yes No Type 4 No Yes

Graph96

Express-Falcon
Falcon-Lady
Bristol-Falcon
Doublol-Cheyenne
Akamar-Apex
Apex-Goeland
Envol-Idol
Goeland-Lady
Apex-Lady
Bristol-Idol
Eurol-Prestol
Andol-Envol
Bristol-Envol
Idol-Goeland
Apex-Bristol
Apex-Idol
Bristol--Lady
Bristol-Goeland
34 9 varieacuteteacutes preacutesentes
193
759
14 8 varieacuteteacutes preacutesentes
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19951996
23
14
3
14
11
10
20
16
9
24
11
18
21
19
12
22
6
13
6
27
10
32
24
14
20
19
16
23
14
19
19
22
21
14
22
19
13
11
16
21
18
19
11
19
17
19
22
26
25
17
19
21
13
24
14
14
14
9
20
9
11
22
14
21
16
5
10
8
10
16
14
6
16
29
20
14
16
16
23
19
18
3
19
14
4
18
11
16
14
12
19
12
8
13
11
6
16
0
5
1
26
8
19
20
10
20
20
9
14
19
9
4
20
13
15
19
11
16
11
2
11
7
5
16
4
1
5
23
19
18
13
16
16
17
10
11
22
20
18
20
18
13
10
13
23
17
22
24
24
20
25
18
20
18
14
13
23
18
20
18
11
20
10
21
9
11
13
18
10
19
19
17
8
16
14
13
14
20
11
12
14
28
16
24
26
14
19
17
23
20
14
11
16
15
13
10
20
14
3
10
10
17
20
15
14
15
15
19
20
11
19
19
17
14
14
22
16
22
22
14
19
10
19
20
10
20
16
20
20
19
18
25
14
19
12
16
16
17
10
26
8
14
13
11
11
16
12
11
17
8
10
16
19
13
13
18
22
21
12
12
10
15
23
16
26
16
22
14
11
26
18
16
5
8
2
22
16
10
20
11
14
13
13
8
9
15
6
1
11
24
18
15
12
17
17
18
13
9
19
14
12
11
13
19
14
10
18
19
11
24
16
14
21
12
8
14
19
12
26
13
22
21
17
11
24
13
21
19
16
23
17
3
20
18
13
14
21
25
24
12
19
15
22
16
16
23
17
19
15
17
25
21
21
10
13
11
24
14
17
20
24
21
18
13
8
8
18
11
14
12
26
23
3
20
14
20
20
16
19
18
8
11
7
24
13
20
19
16
25
22
8
8
4
15
11
9
8
31
19
13
14
10
24
23
10
12
19
10
6
5
20
14
15
18
14
24
21
9
8
4
16
6
8
5
29
12
10
14
7
26
23
7
11
12
12
14
15
15
16
16
16
16
16
16
16
16
18
20
21
21
21
22
22
22
23
24
25
25
27
6
19
14
0
4
18
11
15
14
12
19
12
6
11
11
6
16
5
1
26
11
18
18
13
17
20
9
13
17
6
5
1
20
12
15
19
8
15
10
1
14
9
8
16
5
8
23
20
20
13
19
16
17
12
6
19
16
1
5
18
14
19
12
14
22
15
11
12
8
5
16
1
8
26
9
16
20
9
20
20
6
27
22
29
26
23
14
28
20
16
19
16
23
24
26
31
29
27
26
23
26
25
26
19
33
19
12
27
10
26
20
8
19
13
16
11
16
12
16
16
18
23
19
12
23
11
20
9
25
24
22
13
16
25
14
32
25
14
19
18
23
24
19
17
26
23
26
15
3
13
10
22
18
20
16
26
24
14
19
18
24
20
24
17
16
20
13
18
26
19
10
13
17
16
12
20
14
14
22
18
13
20
19
22
14
28
9
21
13
14
19
16
10
16
20
14
17
26
22
19
22
17
14
10
7
16
13
19
9
33
13
19
28
25
22
15
20
21
23
20
16
18
19
14
8
21
15
14
17
20
24
26
24
17
16
20
19
16
18
9
25
13
20
19
13
19
20
17
11
17
14
14
17
17
11
18
20
23
23
21
20
17
20
12
25
24
21
22
13
25
16
24
18
9
10
20
23
22
13
11
25
26
13
16
10
7
21
9
12
6
27
14
20
13
15
20
25

Graph97

Apex-Goeland
Eurol-Prestol
Eurol-Andol
Apex-Envol
Apex-Express
Envol-Lady
Akamar-Doublol
Contact-Navajo
Ceres-Goeland
Ceres-Lady
Apex-Ceres
Columbus-Tapidor
Goeland-Lady
Apex-Lady
212
16 8 varieacuteteacutes preacutesentes
16 10 varieacuteteacutes preacutesentes
756
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19961997
18
13
13
18
9
17
24
9
6
4
8
13
15
13
9
10
8
13
10
20
8
15
16
20
6
12
13
18
13
19
14
19
19
15
24
24
20
17
22
17
18
22
17
20
17
17
12
18
18
21
11
20
7
24
13
13
15
18
21
20
18
22
18
18
19
9
3
12
18
15
18
13
9
15
18
9
19
18
18
13
19
13
19
15
19
15
4
12
15
12
16
16
5
14
2
8
8
3
17
6
25
10
16
23
10
15
15
8
18
14
18
19
19
17
15
17
13
22
13
21
16
18
19
21
10
9
11
15
17
16
15
26
14
10
19
9
19
21
15
19
25
26
15
13
9
9
22
23
16
11
16
15
19
17
23
14
21
17
18
11
17
15
17
19
20
4
17
25
10
21
11
15
26
12
10
12
10
11
5
16
5
24
18
17
24
20
18
15
21
24
15
18
12
15
26
10
18
20
22
23
21
13
15
20
13
10
16
12
15
20
13
18
11
17
12
18
9
24
22
15
17
15
21
18
14
12
15
23
22
15
22
17
9
20
20
16
11
18
12
24
5
19
16
6
24
18
12
13
13
11
20
14
7
8
13
14
18
6
11
10
8
8
14
3
16
15
21
12
13
20
4
20
18
16
22
9
15
22
12
7
10
19
18
20
9
10
16
17
15
14
10
18
16
23
10
15
22
8
17
19
16
13
9
26
23
15
8
10
17
23
19
12
22
19
9
14
13
9
23
12
25
15
13
18
13
22
9
5
21
22
12
21
23
13
19
17
11
10
12
11
8
10
1
20
12
16
24
21
19
19
21
15
17
3
14
16
23
10
13
22
14
18
23
11
16
13
12
14
10
8
17
11
2
19
20
20
17
26
13
18
12
2
18
16
12
15
15
18
20
19
10
16
15
15
8
18
11
29
9
18
22
8
18
19
6
9
22
18
8
19
11
10
20
22
6
9
12
12
13
15
13
11
11
7
22
7
16
22
18
19
15
22
10
17
15
8
21
16
11
13
17
11
10
22
11
12
15
13
8
17
11
23
11
13
19
20
16
19
22
8
20
18
3
10
15
5
10
9
10
16
19
8
14
8
11
8
15
9
23
11
15
21
19
12
12
10
13
17
13
17
9
19
16
16
20
8
17
9
10
10
18
11
17
15
8
10
8
11
10
24
19
15
23
10
17
9
6
11
17
5
12
20
8
15
14
1
8
11
7
11
9
8
17
13
17
20
21
15
10
16
20
12
15
25
15
23
24
15
16
14
14
13
20
17
29
22
23
23
10
17
15
17
14
23
19
13
30
8
18
18
10
17
14
18
20
11
3
10
9
12
11
9
7
11
11
8
13
15
6
10
15
15
20
19
15
18
9
16
16
21
17
13
18
16
18
23
16
2
18
16
13
15
11
17
17
6
13
20
18
20
25
16
21
19
23
15
17
24
18
12
15
16
12
24
19
22
22
19
21
10
20
14
10
13
26
9
24
21
20
11
18
10
26
18
20
11
24
21
23
25
21
20
8
18
20
19
24
21
23
15
20
26
26
15
14
6
20
18
15
14
11
18
17
5
12
10
15
19
20
18
19
16
12
19
15
19
15
18
9
26
16
14
12
7
13
15
10
17
15
12
19
13
15
13
19
17
19
15
19
12
15
10
13
20
20
24
15
16
21
13
24
19
8
19
15
21
18
16
20
22
18
21
26
6
22
22
10
23
16
30
19
25
21
14
14
21

Graph98

Type 3
Type 1
Type 4
Type 2
Express - Lady
Duetol - Tapidor
Eurol - Zorro
Ceres - Cheyenne
Express - Zorro
Zorro - Apex
Goeland - Lady
Apex - Goeland
Apex - Lady
Eurol - Idol
Bristol - Idol
Andol - Idol
Bristol - Eurol
Apex - Express
Bristol - Prestol
Andol - Eurol
Express - Goeland
Rogers Distances (Molecular marker distances)
GAIumlA Distances(Traditional characteristic distances)
GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998
24
10
12
28
7
12
16
24
14
19
19
18
18
17
11
20
12
19
17
23
6
28
27
17
17
14
17
24
19
15
15
30
11
13
29
30
20
18
20
15
12
24
25
18
17
15
20
29
22
19
13
20
14
17
10
19
8
8
25
9
10
21
24
26
15
12
5
15
9
20
9
1
11
24
15
17
15
9
24
17
11
12
15
8
10
23
7
6
22
21
24
9
11
8
2
10
24
1
7
1
20
15
19
19
8
12
18
5
28
15
8
10
32
14
10
15
32
27
11
9
2
8
16
29
10
1
7
22
29
5
7
10
12
25
8
7
30
25
23
32
25
28
31
17
18
21
30
27
27
20
34
23
26
25
24
8
29
37
30
17
24
25
12
11
9
7
14
25
4
21
22
18
8
18
11
12
11
19
10
14
10
20
18
24
17
10
18
10
13
16
13
10
6
10
28
4
17
32
19
6
10
7
7
16
25
9
6
6
20
21
16
19
7
14
15
9
24
29
21
22
15
31
21
17
28
19
7
20
21
21
20
30
25
21
23
26
34
22
26
24
11
23
19
14
30
24
21
32
17
22
32
28
16
23
29
24
24
23
32
22
24
23
14
14
30
29
30
16
14
18
19
20
26
24
27
18
18
19
19
16
15
21
22
27
31
30
29
26
28
16
21
24
27
27
10
8
20
19
18
15
9
11
21
8
6
7
23
15
7
9
10
16
24
11
10
8
14
25
16
17
9
3
14
9
18
20
12
11
9
30
18
10
20
29
21
7
7
11
23
23
16
11
12
18
22
10
17
11
13
21
7
18
15
5
8
2
27
11
7
21
24
22
9
7
12
16
22
9
1
7
17
22
8
11
9
11
16
4
17
12
15
2
8
27
12
7
21
24
27
10
11
12
12
27
5
10
1
18
21
13
17
15
8
22
4
11
24
9
10
16
20
11
16
20
23
31
16
23
16
12
16
7
13
9
29
12
25
22
11
21
24
15
20
25
20
24
29
34
19
25
30
32
30
24
23
22
27
16
19
22
22
33
26
35
32
8
32
18
26
12
18
9
1
10
23
10
9
25
22
29
11
16
9
5
7
19
8
0
25
14
21
25
10
14
20
8
19
17
1
7
1
26
14
6
21
24
26
10
11
1
10
13
22
8
8
20
22
12
14
12
16
20
7
17
15
11
1
7
25
10
6
23
23
28
8
12
7
1
9
22
0
8
22
20
19
19
9
11
21
6
23
20
24
20
22
24
20
20
26
14
16
14
18
17
18
29
33
25
20
22
25
22
21
25
13
16
17
6
29
15
15
29
8
18
21
34
14
21
25
22
22
21
12
26
14
22
20
25
26
30
22
23
19
19
28
22
17
19
5
29
24
16
22
30
24
16
10
8
13
25
35
21
12
19
22
26
7
23
14
27
13
27
19
15
19
7
37
17
19
26
29
27
17
17
11
17
22
32
25
14
19
21
30
7
20
16
23
12
17
13
9
8
10
30
10
7
24
30
27
9
11
9
15
11
8
10
12
9
25
22
23
20
22
13
13
17
20
24
12
12
17
18
14
11
16
10
3
13
11
8
21
32
14
16
11
13
23
14
16
22
20
9
14
14
17
18
25
24
10
15
23
14
8
14
21
16
22
24
18
20
20
21
16
19
27
23
13
20
17
17
17
11
5
8
25
13
9
19
18
20
9
7
4
4
15
26
8
7
6
17
19
13
12
13
9
17

Graph99

169
677
61 20 varieacuteteacutes preacutesentes
93 19 varieacuteteacutes preacutesentes
Goeland-Lady
Goeland-Apex
Envol-Idol
Apex-Lady
Ceres-Falcon
Bristol-Eurol
Apex-Envol
Andol-Eurol
Eurol-Idol
Bristol-Prestol
Doublol-Duetol
Capitol-Vivol
Eurol-Samourai
Bristol-Samourai
Akamar-Duetol
Contact-Envol
Duetol-Navajo
Apex-Contact
Prestol-Samouraiet Goeland-Idol
Andol-Zorro
Falcon-Navajo
Andol-Falcon
Falcon-Goeland
Lady-Navajo
Duetol-Lady
Express-Zorro
Contact-Goeland
Duetol-Lady
Distances de ROGERS
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19981999
1
1
1
1
0
0
11
11
1
1
2
2
13
13
5
5
8
8
8
8
6
6
5
5
3
7
3
7
8
10
8
10
8
8
5
5
15
15
15
15
6
6
6
6
5
5
13
13
1
1
14
14
18
18
3
3
2
2
3
3
15
15
12
12
13
13
8
8
12
18
12
18
17
17
20
20
12
12
24
2
24
2
5
20
2
20
5
11
2
11
13
13
1
1
20
20
3
16
3
16
15
15
2
8
8
2
8
8
11
11
12
12
15
15
2
4
4
2
8
13
8
13
17
19
19
17
13
13
11
14
11
14
13
13
12
17
8
12
7
20
20
7
17
8
8
8
11
11
9
9
9
9
12
18
12
18
8
8
15
15
15
15
10
10
5
5
8
8
20
6
6
20
13
13
2
15
15
2
6
6
10
10
16
16
14
6
14
6
15
21
15
21
7
7
21
21
20
7
7
20
15
15
7
7
14
6
6
9
2
2
14
9
10
10
16
16
15
15
13
6
13
6
11
11
19
8
10
10
22
8
22
19
5
5
4
5
4
5
5
7
5
15
5
12
15
12
7
5
0
12
6
0
6
12
22
11
14
14
13
22
11
13
10
6
20
6
20
10
4
15
1
4
1
8
11
15
8
11
18
18
13
15
13
15
13
13
5
17
5
9
3
3
15
12
15
5
12
5
17
9
12
12
14
14
8
10
13
10
13
8
23
6
12
9
6
9
12
23
14
12
10
12
14
10
14
14
16
12
13
12
10
13
10
16
12
15
13
12
9
10
9
5
15
13
2
5
2
13
10
13
4
9
17
9
4
17
10
12
9
10
12
9
16
8
8
12
8
16
12
8
13
13
6
3
6
3
11
12
3
20
12
20
14
11
3
14
18
7
7
18
19
19
13
19
19
11
13
11
3
18
2
20
28
3
20
2
28
12
18
12
5
13
8
12
13
5
8
8
12
8
13
9
13
12
9
16
12
18
18
16
10
10
16
15
16
15
14
11
14
11
10
16
8
16
10
8
10
2
8
12
2
6
11
23
10
6
19
8
11
23
12
19
6
6
10
7
8
1
9
21
9
21
7
1
10
8
18
14
18
14
17
17
8
8
9
5
14
3
9
21
5
16
13
14
16
13
21
3
10
5
5
10
13
13
4
15
4
15
5
8
8
8
5
8
5
5
17
15
17
15
10
8
7
8
10
9
20
20
7
9
15
12
10
15
18
12
18
10
15
23
15
23
12
12
19
19
14
14
14
11
11
8
14
8
17
6
17
6
13
13
10
2
2
5
18
10
5
18
10
7
10
13
15
13
15
15
13
15
13
7
2
2
8
8
14
7
10
14
7
10
11
14
11
18
14
18
13
13
16
16
15
16
16
15
9
2
11
2
9
12
12
11
12
15
12
15
8
11
12
14
14
8
12
11
11
24
11
24
13
13
13
15
16
16
22
13
15
16
22
13
15
15
16
14
13
12
14
12
10
10
15
10
6
10
15
6
9
9
13
13
10
11
11
10
14
14
10
10
17
8
7
8
8
17
8
7
8
8
12
2
2
12
17
17
12
12
18
18
18
18
5
8
5
16
16
8
14
14
14
8
22
22
13
13
14
14
9
14
9
8
13
13
11
13
11
13
9
9
12
12
9
9
13
13
10
10
13
13
6
6
11
11
17
17
9
9
15
15
9
9
6
6
12
12
24
24
8
8
12
19
12
19
9
9
12
12

Model 1 Characteristic-specific molecular markers

Example 1 gene specific marker for herbicide tolerance introduced by genetic modification On the basis that [hellip] - there is verification of the reliability of the link between the marker and the characteristic - different markers for the same characteristic are different methods for examining the same characteristic [hellip]

Fusarium race 0 ex 1 Example Characteristic-specific molecular marker with incomplete information on state of expression

Model 1 Characteristic-specific molecular markers

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

ex Maize parent lines

Model 2 Combining phenotypic and molecular distances in the management of variety collections

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

2

02

Model 2 Combining phenotypic and molecular distances in the management of variety collections (continued)

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

13

New Model 3 Genetic selection of similar varieties for the first growing cycle

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
0038
0038
0042
0042
0045
0045
0063
0063
0067
0067
0068
0068
0075
0075
0106
0106
011
011
0114
0114
0115
0115
0116
0116
0123
0123
0123
0123
0131
0131
0131
0131
0132
0132
0145
0145
0149
0149
0149
0149
017
017
0176
0176
018
018
0185
0185
0186
0186
0194
0194
0198
0198
0199
0199
02
02
0201
0201
0202
0202
0203
0203
0204
0204
0207
0207
021
021
021
021
0213
0213
0214
0214
0217
0217
0217
0217
0217
0217
0219
0219
0219
0219
0219
0219
0219
0219
022
022
0224
0224
0225
0225
0225
0225
0225
0225
0226
0226
0227
0227
0227
0227
0227
0227
0228
0228
0228
0228
0228
0228
023
023
023
023
023
023
023
023
0231
0231
0231
0231
0232
0232
0232
0232
0232
0232
0233
0233
0235
0235
0235
0235
0235
0235
0235
0235
0235
0235
0236
0236
0236
0236
0238
0238
0238
0238
0239
0239
0239
0239
0241
0241
0242
0242
0244
0244
0245
0245
0246
0246
0247
0247
0249
0249
0249
0249
025
025
0251
0251
0251
0251
0252
0252
0252
0252
0252
0252
0253
0253
0253
0253
0254
0254
0254
0254
0255
0255
0259
0259
0259
0259
0259
0259
026
026
026
026
0262
0262
0262
0262
0262
0262
0262
0262
0264
0264
0265
0265
0266
0266
0267
0267
0267
0267
0268
0268
0269
0269
0269
0269
0269
0269
0269
0269
027
027
027
027
027
027
0272
0272
0272
0272
0272
0272
0272
0272
0272
0272
0273
0273
0273
0273
0273
0273
0275
0275
0275
0275
0275
0275
0275
0275
0276
0276
0276
0276
0276
0276
0278
0278
0278
0278
0278
0278
0278
0278
0278
0278
0279
0279
0279
0279
0279
0279
028
028
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0282
0282
0282
0282
0283
0283
0283
0283
0283
0283
0284
0284
0284
0284
0284
0284
0284
0284
0285
0285
0285
0285
0285
0285
0285
0285
0286
0286
0286
0286
0286
0286
0286
0286
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0288
0288
0288
0288
0288
0288
0289
0289
0289
0289
0289
0289
029
029
029
029
029
029
029
029
0291
0291
0292
0292
0292
0292
0293
0293
0293
0293
0293
0293
0293
0293
0293
0293
0294
0294
0294
0294
0294
0294
0295
0295
0295
0295
0295
0295
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0298
0298
0298
0298
0298
0298
0298
0298
0298
0298
0299
0299
0299
0299
0299
0299
0299
0299
0299
0299
03
03
0302
0302
0302
0302
0303
0303
0303
0303
0304
0304
0304
0304
0304
0304
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0307
0307
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
031
031
031
031
031
031
0311
0311
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0313
0313
0313
0313
0314
0314
0315
0315
0315
0315
0316
0316
0316
0316
0316
0316
0318
0318
0318
0318
0318
0318
0319
0319
0319
0319
0319
0319
0319
0319
0319
0319
032
032
032
032
032
032
032
032
0321
0321
0321
0321
0322
0322
0322
0322
0323
0323
0324
0324
0324
0324
0324
0324
0325
0325
0325
0325
0326
0326
0327
0327
0327
0327
0327
0327
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0282
0284
0272
0342
0405
0312
0273
0149
0336
027
0278
0345
0068
0299
0285
0312
025
0284
0294
0309
0327
0231
0262
0287
0296
0269
0358
0342
029
0265
0304
0335
0287
0245
0239
034
0213
0312
0351
0225
0235
0217
0253
0259
0293
0281
0309
0293
0231
0198
0296
0219
0296
0299
0342
0306
0207
032
0345
021
027
028
0272
0038
0227
0358
0281
0236
0202
0246
0275
0288
0217
0249
023
0262
0198
0201
0045
0319
0313
0244
0236
0204
0285
0321
0304
0289
0289
0123
0219
0114
0348
0306
0346
0294
0296
029
0306
0042
0106
0281
0287
0296
0201
0225
0315
038
0063
0194
0367
0225
0364
0352
0292
0298
0287
0067
0247
0351
0309
0269
0232
0281
0299
0315
023
0276
0253
0296
0219
0045
0225
0299
0327
0251
0232
0228
031
0335
0324
0295
0252
0333
0327
0306
0275
0275
0302
0254
0302
0185
0231
0288
0322
0398
0269
0296
0319
0315
0299
0361
0339
0294
0318
0354
0316
0321
0292
0342
0404
0298
0383
0351
0327
033
0331
0306
0312
0309
0377
0412
0389
0358
0299
0313
038
0327
0361
0395
0368
0272
0389
0332
0336
0312
0312
0132
0242
0116
0364
0357
0376
034
0342
0323
032
0075
0115
0282
0342
0342
0244
0063
0251
0339
0395
0199
0361
0232
0338
037
0318
0282
0235
0233
02
0359
0281
0342
0285
029
0287
0306
021
0219
0284
029
0306
0236
0194
0232
0294
0368
0199
0342
0235
0338
0303
023
0267
038
0203
0346
0364
0278
0176
0214
0281
0312
029
0345
0329
0272
0265
0207
0204
0367
0228
0318
0272
0361
0342
0358
0345
0287
0283
0325
0266
0293
0259
0361
031
0361
0356
0339
0326
0339
0239
0276
0342
0304
032
0285
0225
031
0354
0389
0232
0235
0358
0342
0373
0296
0287
0386
0329
0329
018
0335
0361
0286
0339
0307
032
0334
0372
0405
0335
0345
0321
0364
0335
0316
0332
0338
0338
0345
0342
031
0295
0252
0352
0298
0336
0342
0269
0235
0279
0235
0284
0287
0329
0319
0312
0287
021
0304
0352
0324
0321
0336
037
0303
0287
0373
031
Page 5: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

6

Technical Working Party

on Automation

and Computer Programs

(TWC)

COUNCIL

ADMINISTRATIVE AND LEGAL COMMITTEE (CAJ) TECHNICAL COMMITTEE (TC)

CONSULTATIVE COMMITTEE

Technical Working Party

for Vegetables

(TWV)

Technical Working Party

for Ornamental Plants and

Forest Trees

(TWO)

Technical Working Party

for Fruit Crops

(TWF)

Technical Working Party

for Agricultural

Crops

(TWA)

Office of the Union

Working Group on Biochemical and

Molecular Techniques and DNA-Profiling in Particular (BMT)

Document reference Title

TGP15 Guidance on the Use of Biochemical and Molecular Markers in the Examination of Distinctness Uniformity and Stability (DUS)

UPOVINF181 Possible Use of Molecular Markers in the Examination of Distinctness Uniformity and Stability (2011)

Document reference Title

UPOVINF171 Guidelines for DNA Profiling Molecular Marker Selection and Database Construction (ldquoBMT Guidelinesrdquo) (2010)

STATUS OF UPOV DOCUMENTS CONCERNING MOLECULAR TECHNIQUES

Calibrated molecular distances in the management of variety collections

8

02468

10121416182022242628303234363840

0 005 01 015 02 025 03 035 04 045

GA

IumlA D

ista

nces

(Tra

ditio

nal c

hara

cter

istic

di

stan

ces)

Rogers Distances (Molecular marker distances)

GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998

Type 3 Type 1

Type 4Type 2Express - Lady

Duetol - TapidorEurol - Zorro

Ceres - Cheyenne

Express - Zorro

Zorro - Apex

Goeland - Lady

Apex - GoelandApex - Lady

Eurol - Idol

Bristol - Idol

Andol - IdolBristol - Eurol Apex - Express

Bristol - Prestol Andol - Eurol Express - Goeland

Distinctness plus (Traditional characteristics)

Distinctness plus (Molecular markers)

Type 1 Yes Yes Type 2 No No Type 3 Yes No Type 4 No Yes

Graph96

Express-Falcon
Falcon-Lady
Bristol-Falcon
Doublol-Cheyenne
Akamar-Apex
Apex-Goeland
Envol-Idol
Goeland-Lady
Apex-Lady
Bristol-Idol
Eurol-Prestol
Andol-Envol
Bristol-Envol
Idol-Goeland
Apex-Bristol
Apex-Idol
Bristol--Lady
Bristol-Goeland
34 9 varieacuteteacutes preacutesentes
193
759
14 8 varieacuteteacutes preacutesentes
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19951996
23
14
3
14
11
10
20
16
9
24
11
18
21
19
12
22
6
13
6
27
10
32
24
14
20
19
16
23
14
19
19
22
21
14
22
19
13
11
16
21
18
19
11
19
17
19
22
26
25
17
19
21
13
24
14
14
14
9
20
9
11
22
14
21
16
5
10
8
10
16
14
6
16
29
20
14
16
16
23
19
18
3
19
14
4
18
11
16
14
12
19
12
8
13
11
6
16
0
5
1
26
8
19
20
10
20
20
9
14
19
9
4
20
13
15
19
11
16
11
2
11
7
5
16
4
1
5
23
19
18
13
16
16
17
10
11
22
20
18
20
18
13
10
13
23
17
22
24
24
20
25
18
20
18
14
13
23
18
20
18
11
20
10
21
9
11
13
18
10
19
19
17
8
16
14
13
14
20
11
12
14
28
16
24
26
14
19
17
23
20
14
11
16
15
13
10
20
14
3
10
10
17
20
15
14
15
15
19
20
11
19
19
17
14
14
22
16
22
22
14
19
10
19
20
10
20
16
20
20
19
18
25
14
19
12
16
16
17
10
26
8
14
13
11
11
16
12
11
17
8
10
16
19
13
13
18
22
21
12
12
10
15
23
16
26
16
22
14
11
26
18
16
5
8
2
22
16
10
20
11
14
13
13
8
9
15
6
1
11
24
18
15
12
17
17
18
13
9
19
14
12
11
13
19
14
10
18
19
11
24
16
14
21
12
8
14
19
12
26
13
22
21
17
11
24
13
21
19
16
23
17
3
20
18
13
14
21
25
24
12
19
15
22
16
16
23
17
19
15
17
25
21
21
10
13
11
24
14
17
20
24
21
18
13
8
8
18
11
14
12
26
23
3
20
14
20
20
16
19
18
8
11
7
24
13
20
19
16
25
22
8
8
4
15
11
9
8
31
19
13
14
10
24
23
10
12
19
10
6
5
20
14
15
18
14
24
21
9
8
4
16
6
8
5
29
12
10
14
7
26
23
7
11
12
12
14
15
15
16
16
16
16
16
16
16
16
18
20
21
21
21
22
22
22
23
24
25
25
27
6
19
14
0
4
18
11
15
14
12
19
12
6
11
11
6
16
5
1
26
11
18
18
13
17
20
9
13
17
6
5
1
20
12
15
19
8
15
10
1
14
9
8
16
5
8
23
20
20
13
19
16
17
12
6
19
16
1
5
18
14
19
12
14
22
15
11
12
8
5
16
1
8
26
9
16
20
9
20
20
6
27
22
29
26
23
14
28
20
16
19
16
23
24
26
31
29
27
26
23
26
25
26
19
33
19
12
27
10
26
20
8
19
13
16
11
16
12
16
16
18
23
19
12
23
11
20
9
25
24
22
13
16
25
14
32
25
14
19
18
23
24
19
17
26
23
26
15
3
13
10
22
18
20
16
26
24
14
19
18
24
20
24
17
16
20
13
18
26
19
10
13
17
16
12
20
14
14
22
18
13
20
19
22
14
28
9
21
13
14
19
16
10
16
20
14
17
26
22
19
22
17
14
10
7
16
13
19
9
33
13
19
28
25
22
15
20
21
23
20
16
18
19
14
8
21
15
14
17
20
24
26
24
17
16
20
19
16
18
9
25
13
20
19
13
19
20
17
11
17
14
14
17
17
11
18
20
23
23
21
20
17
20
12
25
24
21
22
13
25
16
24
18
9
10
20
23
22
13
11
25
26
13
16
10
7
21
9
12
6
27
14
20
13
15
20
25

Graph97

Apex-Goeland
Eurol-Prestol
Eurol-Andol
Apex-Envol
Apex-Express
Envol-Lady
Akamar-Doublol
Contact-Navajo
Ceres-Goeland
Ceres-Lady
Apex-Ceres
Columbus-Tapidor
Goeland-Lady
Apex-Lady
212
16 8 varieacuteteacutes preacutesentes
16 10 varieacuteteacutes preacutesentes
756
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19961997
18
13
13
18
9
17
24
9
6
4
8
13
15
13
9
10
8
13
10
20
8
15
16
20
6
12
13
18
13
19
14
19
19
15
24
24
20
17
22
17
18
22
17
20
17
17
12
18
18
21
11
20
7
24
13
13
15
18
21
20
18
22
18
18
19
9
3
12
18
15
18
13
9
15
18
9
19
18
18
13
19
13
19
15
19
15
4
12
15
12
16
16
5
14
2
8
8
3
17
6
25
10
16
23
10
15
15
8
18
14
18
19
19
17
15
17
13
22
13
21
16
18
19
21
10
9
11
15
17
16
15
26
14
10
19
9
19
21
15
19
25
26
15
13
9
9
22
23
16
11
16
15
19
17
23
14
21
17
18
11
17
15
17
19
20
4
17
25
10
21
11
15
26
12
10
12
10
11
5
16
5
24
18
17
24
20
18
15
21
24
15
18
12
15
26
10
18
20
22
23
21
13
15
20
13
10
16
12
15
20
13
18
11
17
12
18
9
24
22
15
17
15
21
18
14
12
15
23
22
15
22
17
9
20
20
16
11
18
12
24
5
19
16
6
24
18
12
13
13
11
20
14
7
8
13
14
18
6
11
10
8
8
14
3
16
15
21
12
13
20
4
20
18
16
22
9
15
22
12
7
10
19
18
20
9
10
16
17
15
14
10
18
16
23
10
15
22
8
17
19
16
13
9
26
23
15
8
10
17
23
19
12
22
19
9
14
13
9
23
12
25
15
13
18
13
22
9
5
21
22
12
21
23
13
19
17
11
10
12
11
8
10
1
20
12
16
24
21
19
19
21
15
17
3
14
16
23
10
13
22
14
18
23
11
16
13
12
14
10
8
17
11
2
19
20
20
17
26
13
18
12
2
18
16
12
15
15
18
20
19
10
16
15
15
8
18
11
29
9
18
22
8
18
19
6
9
22
18
8
19
11
10
20
22
6
9
12
12
13
15
13
11
11
7
22
7
16
22
18
19
15
22
10
17
15
8
21
16
11
13
17
11
10
22
11
12
15
13
8
17
11
23
11
13
19
20
16
19
22
8
20
18
3
10
15
5
10
9
10
16
19
8
14
8
11
8
15
9
23
11
15
21
19
12
12
10
13
17
13
17
9
19
16
16
20
8
17
9
10
10
18
11
17
15
8
10
8
11
10
24
19
15
23
10
17
9
6
11
17
5
12
20
8
15
14
1
8
11
7
11
9
8
17
13
17
20
21
15
10
16
20
12
15
25
15
23
24
15
16
14
14
13
20
17
29
22
23
23
10
17
15
17
14
23
19
13
30
8
18
18
10
17
14
18
20
11
3
10
9
12
11
9
7
11
11
8
13
15
6
10
15
15
20
19
15
18
9
16
16
21
17
13
18
16
18
23
16
2
18
16
13
15
11
17
17
6
13
20
18
20
25
16
21
19
23
15
17
24
18
12
15
16
12
24
19
22
22
19
21
10
20
14
10
13
26
9
24
21
20
11
18
10
26
18
20
11
24
21
23
25
21
20
8
18
20
19
24
21
23
15
20
26
26
15
14
6
20
18
15
14
11
18
17
5
12
10
15
19
20
18
19
16
12
19
15
19
15
18
9
26
16
14
12
7
13
15
10
17
15
12
19
13
15
13
19
17
19
15
19
12
15
10
13
20
20
24
15
16
21
13
24
19
8
19
15
21
18
16
20
22
18
21
26
6
22
22
10
23
16
30
19
25
21
14
14
21

Graph98

Type 3
Type 1
Type 4
Type 2
Express - Lady
Duetol - Tapidor
Eurol - Zorro
Ceres - Cheyenne
Express - Zorro
Zorro - Apex
Goeland - Lady
Apex - Goeland
Apex - Lady
Eurol - Idol
Bristol - Idol
Andol - Idol
Bristol - Eurol
Apex - Express
Bristol - Prestol
Andol - Eurol
Express - Goeland
Rogers Distances (Molecular marker distances)
GAIumlA Distances(Traditional characteristic distances)
GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998
24
10
12
28
7
12
16
24
14
19
19
18
18
17
11
20
12
19
17
23
6
28
27
17
17
14
17
24
19
15
15
30
11
13
29
30
20
18
20
15
12
24
25
18
17
15
20
29
22
19
13
20
14
17
10
19
8
8
25
9
10
21
24
26
15
12
5
15
9
20
9
1
11
24
15
17
15
9
24
17
11
12
15
8
10
23
7
6
22
21
24
9
11
8
2
10
24
1
7
1
20
15
19
19
8
12
18
5
28
15
8
10
32
14
10
15
32
27
11
9
2
8
16
29
10
1
7
22
29
5
7
10
12
25
8
7
30
25
23
32
25
28
31
17
18
21
30
27
27
20
34
23
26
25
24
8
29
37
30
17
24
25
12
11
9
7
14
25
4
21
22
18
8
18
11
12
11
19
10
14
10
20
18
24
17
10
18
10
13
16
13
10
6
10
28
4
17
32
19
6
10
7
7
16
25
9
6
6
20
21
16
19
7
14
15
9
24
29
21
22
15
31
21
17
28
19
7
20
21
21
20
30
25
21
23
26
34
22
26
24
11
23
19
14
30
24
21
32
17
22
32
28
16
23
29
24
24
23
32
22
24
23
14
14
30
29
30
16
14
18
19
20
26
24
27
18
18
19
19
16
15
21
22
27
31
30
29
26
28
16
21
24
27
27
10
8
20
19
18
15
9
11
21
8
6
7
23
15
7
9
10
16
24
11
10
8
14
25
16
17
9
3
14
9
18
20
12
11
9
30
18
10
20
29
21
7
7
11
23
23
16
11
12
18
22
10
17
11
13
21
7
18
15
5
8
2
27
11
7
21
24
22
9
7
12
16
22
9
1
7
17
22
8
11
9
11
16
4
17
12
15
2
8
27
12
7
21
24
27
10
11
12
12
27
5
10
1
18
21
13
17
15
8
22
4
11
24
9
10
16
20
11
16
20
23
31
16
23
16
12
16
7
13
9
29
12
25
22
11
21
24
15
20
25
20
24
29
34
19
25
30
32
30
24
23
22
27
16
19
22
22
33
26
35
32
8
32
18
26
12
18
9
1
10
23
10
9
25
22
29
11
16
9
5
7
19
8
0
25
14
21
25
10
14
20
8
19
17
1
7
1
26
14
6
21
24
26
10
11
1
10
13
22
8
8
20
22
12
14
12
16
20
7
17
15
11
1
7
25
10
6
23
23
28
8
12
7
1
9
22
0
8
22
20
19
19
9
11
21
6
23
20
24
20
22
24
20
20
26
14
16
14
18
17
18
29
33
25
20
22
25
22
21
25
13
16
17
6
29
15
15
29
8
18
21
34
14
21
25
22
22
21
12
26
14
22
20
25
26
30
22
23
19
19
28
22
17
19
5
29
24
16
22
30
24
16
10
8
13
25
35
21
12
19
22
26
7
23
14
27
13
27
19
15
19
7
37
17
19
26
29
27
17
17
11
17
22
32
25
14
19
21
30
7
20
16
23
12
17
13
9
8
10
30
10
7
24
30
27
9
11
9
15
11
8
10
12
9
25
22
23
20
22
13
13
17
20
24
12
12
17
18
14
11
16
10
3
13
11
8
21
32
14
16
11
13
23
14
16
22
20
9
14
14
17
18
25
24
10
15
23
14
8
14
21
16
22
24
18
20
20
21
16
19
27
23
13
20
17
17
17
11
5
8
25
13
9
19
18
20
9
7
4
4
15
26
8
7
6
17
19
13
12
13
9
17

Graph99

169
677
61 20 varieacuteteacutes preacutesentes
93 19 varieacuteteacutes preacutesentes
Goeland-Lady
Goeland-Apex
Envol-Idol
Apex-Lady
Ceres-Falcon
Bristol-Eurol
Apex-Envol
Andol-Eurol
Eurol-Idol
Bristol-Prestol
Doublol-Duetol
Capitol-Vivol
Eurol-Samourai
Bristol-Samourai
Akamar-Duetol
Contact-Envol
Duetol-Navajo
Apex-Contact
Prestol-Samouraiet Goeland-Idol
Andol-Zorro
Falcon-Navajo
Andol-Falcon
Falcon-Goeland
Lady-Navajo
Duetol-Lady
Express-Zorro
Contact-Goeland
Duetol-Lady
Distances de ROGERS
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19981999
1
1
1
1
0
0
11
11
1
1
2
2
13
13
5
5
8
8
8
8
6
6
5
5
3
7
3
7
8
10
8
10
8
8
5
5
15
15
15
15
6
6
6
6
5
5
13
13
1
1
14
14
18
18
3
3
2
2
3
3
15
15
12
12
13
13
8
8
12
18
12
18
17
17
20
20
12
12
24
2
24
2
5
20
2
20
5
11
2
11
13
13
1
1
20
20
3
16
3
16
15
15
2
8
8
2
8
8
11
11
12
12
15
15
2
4
4
2
8
13
8
13
17
19
19
17
13
13
11
14
11
14
13
13
12
17
8
12
7
20
20
7
17
8
8
8
11
11
9
9
9
9
12
18
12
18
8
8
15
15
15
15
10
10
5
5
8
8
20
6
6
20
13
13
2
15
15
2
6
6
10
10
16
16
14
6
14
6
15
21
15
21
7
7
21
21
20
7
7
20
15
15
7
7
14
6
6
9
2
2
14
9
10
10
16
16
15
15
13
6
13
6
11
11
19
8
10
10
22
8
22
19
5
5
4
5
4
5
5
7
5
15
5
12
15
12
7
5
0
12
6
0
6
12
22
11
14
14
13
22
11
13
10
6
20
6
20
10
4
15
1
4
1
8
11
15
8
11
18
18
13
15
13
15
13
13
5
17
5
9
3
3
15
12
15
5
12
5
17
9
12
12
14
14
8
10
13
10
13
8
23
6
12
9
6
9
12
23
14
12
10
12
14
10
14
14
16
12
13
12
10
13
10
16
12
15
13
12
9
10
9
5
15
13
2
5
2
13
10
13
4
9
17
9
4
17
10
12
9
10
12
9
16
8
8
12
8
16
12
8
13
13
6
3
6
3
11
12
3
20
12
20
14
11
3
14
18
7
7
18
19
19
13
19
19
11
13
11
3
18
2
20
28
3
20
2
28
12
18
12
5
13
8
12
13
5
8
8
12
8
13
9
13
12
9
16
12
18
18
16
10
10
16
15
16
15
14
11
14
11
10
16
8
16
10
8
10
2
8
12
2
6
11
23
10
6
19
8
11
23
12
19
6
6
10
7
8
1
9
21
9
21
7
1
10
8
18
14
18
14
17
17
8
8
9
5
14
3
9
21
5
16
13
14
16
13
21
3
10
5
5
10
13
13
4
15
4
15
5
8
8
8
5
8
5
5
17
15
17
15
10
8
7
8
10
9
20
20
7
9
15
12
10
15
18
12
18
10
15
23
15
23
12
12
19
19
14
14
14
11
11
8
14
8
17
6
17
6
13
13
10
2
2
5
18
10
5
18
10
7
10
13
15
13
15
15
13
15
13
7
2
2
8
8
14
7
10
14
7
10
11
14
11
18
14
18
13
13
16
16
15
16
16
15
9
2
11
2
9
12
12
11
12
15
12
15
8
11
12
14
14
8
12
11
11
24
11
24
13
13
13
15
16
16
22
13
15
16
22
13
15
15
16
14
13
12
14
12
10
10
15
10
6
10
15
6
9
9
13
13
10
11
11
10
14
14
10
10
17
8
7
8
8
17
8
7
8
8
12
2
2
12
17
17
12
12
18
18
18
18
5
8
5
16
16
8
14
14
14
8
22
22
13
13
14
14
9
14
9
8
13
13
11
13
11
13
9
9
12
12
9
9
13
13
10
10
13
13
6
6
11
11
17
17
9
9
15
15
9
9
6
6
12
12
24
24
8
8
12
19
12
19
9
9
12
12

Model 1 Characteristic-specific molecular markers

Example 1 gene specific marker for herbicide tolerance introduced by genetic modification On the basis that [hellip] - there is verification of the reliability of the link between the marker and the characteristic - different markers for the same characteristic are different methods for examining the same characteristic [hellip]

Fusarium race 0 ex 1 Example Characteristic-specific molecular marker with incomplete information on state of expression

Model 1 Characteristic-specific molecular markers

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

ex Maize parent lines

Model 2 Combining phenotypic and molecular distances in the management of variety collections

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

2

02

Model 2 Combining phenotypic and molecular distances in the management of variety collections (continued)

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

13

New Model 3 Genetic selection of similar varieties for the first growing cycle

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
0038
0038
0042
0042
0045
0045
0063
0063
0067
0067
0068
0068
0075
0075
0106
0106
011
011
0114
0114
0115
0115
0116
0116
0123
0123
0123
0123
0131
0131
0131
0131
0132
0132
0145
0145
0149
0149
0149
0149
017
017
0176
0176
018
018
0185
0185
0186
0186
0194
0194
0198
0198
0199
0199
02
02
0201
0201
0202
0202
0203
0203
0204
0204
0207
0207
021
021
021
021
0213
0213
0214
0214
0217
0217
0217
0217
0217
0217
0219
0219
0219
0219
0219
0219
0219
0219
022
022
0224
0224
0225
0225
0225
0225
0225
0225
0226
0226
0227
0227
0227
0227
0227
0227
0228
0228
0228
0228
0228
0228
023
023
023
023
023
023
023
023
0231
0231
0231
0231
0232
0232
0232
0232
0232
0232
0233
0233
0235
0235
0235
0235
0235
0235
0235
0235
0235
0235
0236
0236
0236
0236
0238
0238
0238
0238
0239
0239
0239
0239
0241
0241
0242
0242
0244
0244
0245
0245
0246
0246
0247
0247
0249
0249
0249
0249
025
025
0251
0251
0251
0251
0252
0252
0252
0252
0252
0252
0253
0253
0253
0253
0254
0254
0254
0254
0255
0255
0259
0259
0259
0259
0259
0259
026
026
026
026
0262
0262
0262
0262
0262
0262
0262
0262
0264
0264
0265
0265
0266
0266
0267
0267
0267
0267
0268
0268
0269
0269
0269
0269
0269
0269
0269
0269
027
027
027
027
027
027
0272
0272
0272
0272
0272
0272
0272
0272
0272
0272
0273
0273
0273
0273
0273
0273
0275
0275
0275
0275
0275
0275
0275
0275
0276
0276
0276
0276
0276
0276
0278
0278
0278
0278
0278
0278
0278
0278
0278
0278
0279
0279
0279
0279
0279
0279
028
028
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0282
0282
0282
0282
0283
0283
0283
0283
0283
0283
0284
0284
0284
0284
0284
0284
0284
0284
0285
0285
0285
0285
0285
0285
0285
0285
0286
0286
0286
0286
0286
0286
0286
0286
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0288
0288
0288
0288
0288
0288
0289
0289
0289
0289
0289
0289
029
029
029
029
029
029
029
029
0291
0291
0292
0292
0292
0292
0293
0293
0293
0293
0293
0293
0293
0293
0293
0293
0294
0294
0294
0294
0294
0294
0295
0295
0295
0295
0295
0295
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0298
0298
0298
0298
0298
0298
0298
0298
0298
0298
0299
0299
0299
0299
0299
0299
0299
0299
0299
0299
03
03
0302
0302
0302
0302
0303
0303
0303
0303
0304
0304
0304
0304
0304
0304
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0307
0307
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
031
031
031
031
031
031
0311
0311
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0313
0313
0313
0313
0314
0314
0315
0315
0315
0315
0316
0316
0316
0316
0316
0316
0318
0318
0318
0318
0318
0318
0319
0319
0319
0319
0319
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0286
0322
0316
011
0252
0309
0309
0249
0106
0276
0322
0412
0115
0219
0329
0276
0372
0319
0311
0329
0293
0241
0278
0422
0333
0287
0341
0324
0361
0352
0288
0252
0327
0293
023
0281
0253
0398
0389
0282
0284
0272
0342
0405
0312
0273
0149
0336
027
0278
0345
0068
0299
0285
0312
025
0284
0294
0309
0327
0231
0262
0287
0296
0269
0358
0342
029
0265
0304
0335
0287
0245
0239
034
0213
0312
0351
0225
0235
0217
0253
0259
0293
0281
0309
0293
0231
0198
0296
0219
0296
0299
0342
0306
0207
032
0345
021
027
028
0272
0038
0227
0358
0281
0236
0202
0246
0275
0288
0217
0249
023
0262
0198
0201
0045
0319
0313
0244
0236
0204
0285
0321
0304
0289
0289
0123
0219
0114
0348
0306
0346
0294
0296
029
0306
0042
0106
0281
0287
0296
0201
0225
0315
038
0063
0194
0367
0225
0364
0352
0292
0298
0287
0067
0247
0351
0309
0269
0232
0281
0299
0315
023
0276
0253
0296
0219
0045
0225
0299
0327
0251
0232
0228
031
0335
0324
0295
0252
0333
0327
0306
0275
0275
0302
0254
0302
0185
0231
0288
0322
0398
0269
0296
0319
0315
0299
0361
0339
0294
0318
0354
0316
0321
0292
0342
0404
0298
0383
0351
0327
033
0331
0306
0312
0309
0377
0412
0389
0358
0299
0313
038
0327
0361
0395
0368
0272
0389
0332
0336
0312
0312
0132
0242
0116
0364
0357
0376
034
0342
0323
032
0075
0115
0282
0342
0342
0244
0063
0251
0339
0395
0199
0361
0232
0338
037
0318
0282
0235
0233
02
0359
0281
0342
0285
029
0287
0306
021
0219
0284
029
0306
0236
0194
0232
0294
0368
0199
0342
0235
0338
0303
023
0267
038
0203
0346
0364
0278
0176
0214
0281
0312
029
0345
0329
0272
0265
0207
0204
0367
0228
0318
0272
0361
0342
0358
0345
0287
0283
0325
0266
0293
0259
0361
031
0361
0356
0339
0326
0339
0239
0276
0342
0304
032
0285
0225
031
0354
0389
0232
0235
0358
0342
0373
0296
0287
0386
0329
0329
018
0335
0361
0286
0339
0307
032
0334
0372
0405
0335
0345
0321
0364
0335
0316
0332
0338
0338
0345
0342
031
0295
0252
0352
0298
0336
0342
0269
0235
0279
0235
0284
0287
0329
0319
0312
0287
021
0304
0352
0324
0321
0336
037
0303
0287
0373
031
Page 6: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

6

Technical Working Party

on Automation

and Computer Programs

(TWC)

COUNCIL

ADMINISTRATIVE AND LEGAL COMMITTEE (CAJ) TECHNICAL COMMITTEE (TC)

CONSULTATIVE COMMITTEE

Technical Working Party

for Vegetables

(TWV)

Technical Working Party

for Ornamental Plants and

Forest Trees

(TWO)

Technical Working Party

for Fruit Crops

(TWF)

Technical Working Party

for Agricultural

Crops

(TWA)

Office of the Union

Working Group on Biochemical and

Molecular Techniques and DNA-Profiling in Particular (BMT)

Document reference Title

TGP15 Guidance on the Use of Biochemical and Molecular Markers in the Examination of Distinctness Uniformity and Stability (DUS)

UPOVINF181 Possible Use of Molecular Markers in the Examination of Distinctness Uniformity and Stability (2011)

Document reference Title

UPOVINF171 Guidelines for DNA Profiling Molecular Marker Selection and Database Construction (ldquoBMT Guidelinesrdquo) (2010)

STATUS OF UPOV DOCUMENTS CONCERNING MOLECULAR TECHNIQUES

Calibrated molecular distances in the management of variety collections

8

02468

10121416182022242628303234363840

0 005 01 015 02 025 03 035 04 045

GA

IumlA D

ista

nces

(Tra

ditio

nal c

hara

cter

istic

di

stan

ces)

Rogers Distances (Molecular marker distances)

GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998

Type 3 Type 1

Type 4Type 2Express - Lady

Duetol - TapidorEurol - Zorro

Ceres - Cheyenne

Express - Zorro

Zorro - Apex

Goeland - Lady

Apex - GoelandApex - Lady

Eurol - Idol

Bristol - Idol

Andol - IdolBristol - Eurol Apex - Express

Bristol - Prestol Andol - Eurol Express - Goeland

Distinctness plus (Traditional characteristics)

Distinctness plus (Molecular markers)

Type 1 Yes Yes Type 2 No No Type 3 Yes No Type 4 No Yes

Graph96

Express-Falcon
Falcon-Lady
Bristol-Falcon
Doublol-Cheyenne
Akamar-Apex
Apex-Goeland
Envol-Idol
Goeland-Lady
Apex-Lady
Bristol-Idol
Eurol-Prestol
Andol-Envol
Bristol-Envol
Idol-Goeland
Apex-Bristol
Apex-Idol
Bristol--Lady
Bristol-Goeland
34 9 varieacuteteacutes preacutesentes
193
759
14 8 varieacuteteacutes preacutesentes
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19951996
23
14
3
14
11
10
20
16
9
24
11
18
21
19
12
22
6
13
6
27
10
32
24
14
20
19
16
23
14
19
19
22
21
14
22
19
13
11
16
21
18
19
11
19
17
19
22
26
25
17
19
21
13
24
14
14
14
9
20
9
11
22
14
21
16
5
10
8
10
16
14
6
16
29
20
14
16
16
23
19
18
3
19
14
4
18
11
16
14
12
19
12
8
13
11
6
16
0
5
1
26
8
19
20
10
20
20
9
14
19
9
4
20
13
15
19
11
16
11
2
11
7
5
16
4
1
5
23
19
18
13
16
16
17
10
11
22
20
18
20
18
13
10
13
23
17
22
24
24
20
25
18
20
18
14
13
23
18
20
18
11
20
10
21
9
11
13
18
10
19
19
17
8
16
14
13
14
20
11
12
14
28
16
24
26
14
19
17
23
20
14
11
16
15
13
10
20
14
3
10
10
17
20
15
14
15
15
19
20
11
19
19
17
14
14
22
16
22
22
14
19
10
19
20
10
20
16
20
20
19
18
25
14
19
12
16
16
17
10
26
8
14
13
11
11
16
12
11
17
8
10
16
19
13
13
18
22
21
12
12
10
15
23
16
26
16
22
14
11
26
18
16
5
8
2
22
16
10
20
11
14
13
13
8
9
15
6
1
11
24
18
15
12
17
17
18
13
9
19
14
12
11
13
19
14
10
18
19
11
24
16
14
21
12
8
14
19
12
26
13
22
21
17
11
24
13
21
19
16
23
17
3
20
18
13
14
21
25
24
12
19
15
22
16
16
23
17
19
15
17
25
21
21
10
13
11
24
14
17
20
24
21
18
13
8
8
18
11
14
12
26
23
3
20
14
20
20
16
19
18
8
11
7
24
13
20
19
16
25
22
8
8
4
15
11
9
8
31
19
13
14
10
24
23
10
12
19
10
6
5
20
14
15
18
14
24
21
9
8
4
16
6
8
5
29
12
10
14
7
26
23
7
11
12
12
14
15
15
16
16
16
16
16
16
16
16
18
20
21
21
21
22
22
22
23
24
25
25
27
6
19
14
0
4
18
11
15
14
12
19
12
6
11
11
6
16
5
1
26
11
18
18
13
17
20
9
13
17
6
5
1
20
12
15
19
8
15
10
1
14
9
8
16
5
8
23
20
20
13
19
16
17
12
6
19
16
1
5
18
14
19
12
14
22
15
11
12
8
5
16
1
8
26
9
16
20
9
20
20
6
27
22
29
26
23
14
28
20
16
19
16
23
24
26
31
29
27
26
23
26
25
26
19
33
19
12
27
10
26
20
8
19
13
16
11
16
12
16
16
18
23
19
12
23
11
20
9
25
24
22
13
16
25
14
32
25
14
19
18
23
24
19
17
26
23
26
15
3
13
10
22
18
20
16
26
24
14
19
18
24
20
24
17
16
20
13
18
26
19
10
13
17
16
12
20
14
14
22
18
13
20
19
22
14
28
9
21
13
14
19
16
10
16
20
14
17
26
22
19
22
17
14
10
7
16
13
19
9
33
13
19
28
25
22
15
20
21
23
20
16
18
19
14
8
21
15
14
17
20
24
26
24
17
16
20
19
16
18
9
25
13
20
19
13
19
20
17
11
17
14
14
17
17
11
18
20
23
23
21
20
17
20
12
25
24
21
22
13
25
16
24
18
9
10
20
23
22
13
11
25
26
13
16
10
7
21
9
12
6
27
14
20
13
15
20
25

Graph97

Apex-Goeland
Eurol-Prestol
Eurol-Andol
Apex-Envol
Apex-Express
Envol-Lady
Akamar-Doublol
Contact-Navajo
Ceres-Goeland
Ceres-Lady
Apex-Ceres
Columbus-Tapidor
Goeland-Lady
Apex-Lady
212
16 8 varieacuteteacutes preacutesentes
16 10 varieacuteteacutes preacutesentes
756
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19961997
18
13
13
18
9
17
24
9
6
4
8
13
15
13
9
10
8
13
10
20
8
15
16
20
6
12
13
18
13
19
14
19
19
15
24
24
20
17
22
17
18
22
17
20
17
17
12
18
18
21
11
20
7
24
13
13
15
18
21
20
18
22
18
18
19
9
3
12
18
15
18
13
9
15
18
9
19
18
18
13
19
13
19
15
19
15
4
12
15
12
16
16
5
14
2
8
8
3
17
6
25
10
16
23
10
15
15
8
18
14
18
19
19
17
15
17
13
22
13
21
16
18
19
21
10
9
11
15
17
16
15
26
14
10
19
9
19
21
15
19
25
26
15
13
9
9
22
23
16
11
16
15
19
17
23
14
21
17
18
11
17
15
17
19
20
4
17
25
10
21
11
15
26
12
10
12
10
11
5
16
5
24
18
17
24
20
18
15
21
24
15
18
12
15
26
10
18
20
22
23
21
13
15
20
13
10
16
12
15
20
13
18
11
17
12
18
9
24
22
15
17
15
21
18
14
12
15
23
22
15
22
17
9
20
20
16
11
18
12
24
5
19
16
6
24
18
12
13
13
11
20
14
7
8
13
14
18
6
11
10
8
8
14
3
16
15
21
12
13
20
4
20
18
16
22
9
15
22
12
7
10
19
18
20
9
10
16
17
15
14
10
18
16
23
10
15
22
8
17
19
16
13
9
26
23
15
8
10
17
23
19
12
22
19
9
14
13
9
23
12
25
15
13
18
13
22
9
5
21
22
12
21
23
13
19
17
11
10
12
11
8
10
1
20
12
16
24
21
19
19
21
15
17
3
14
16
23
10
13
22
14
18
23
11
16
13
12
14
10
8
17
11
2
19
20
20
17
26
13
18
12
2
18
16
12
15
15
18
20
19
10
16
15
15
8
18
11
29
9
18
22
8
18
19
6
9
22
18
8
19
11
10
20
22
6
9
12
12
13
15
13
11
11
7
22
7
16
22
18
19
15
22
10
17
15
8
21
16
11
13
17
11
10
22
11
12
15
13
8
17
11
23
11
13
19
20
16
19
22
8
20
18
3
10
15
5
10
9
10
16
19
8
14
8
11
8
15
9
23
11
15
21
19
12
12
10
13
17
13
17
9
19
16
16
20
8
17
9
10
10
18
11
17
15
8
10
8
11
10
24
19
15
23
10
17
9
6
11
17
5
12
20
8
15
14
1
8
11
7
11
9
8
17
13
17
20
21
15
10
16
20
12
15
25
15
23
24
15
16
14
14
13
20
17
29
22
23
23
10
17
15
17
14
23
19
13
30
8
18
18
10
17
14
18
20
11
3
10
9
12
11
9
7
11
11
8
13
15
6
10
15
15
20
19
15
18
9
16
16
21
17
13
18
16
18
23
16
2
18
16
13
15
11
17
17
6
13
20
18
20
25
16
21
19
23
15
17
24
18
12
15
16
12
24
19
22
22
19
21
10
20
14
10
13
26
9
24
21
20
11
18
10
26
18
20
11
24
21
23
25
21
20
8
18
20
19
24
21
23
15
20
26
26
15
14
6
20
18
15
14
11
18
17
5
12
10
15
19
20
18
19
16
12
19
15
19
15
18
9
26
16
14
12
7
13
15
10
17
15
12
19
13
15
13
19
17
19
15
19
12
15
10
13
20
20
24
15
16
21
13
24
19
8
19
15
21
18
16
20
22
18
21
26
6
22
22
10
23
16
30
19
25
21
14
14
21

Graph98

Type 3
Type 1
Type 4
Type 2
Express - Lady
Duetol - Tapidor
Eurol - Zorro
Ceres - Cheyenne
Express - Zorro
Zorro - Apex
Goeland - Lady
Apex - Goeland
Apex - Lady
Eurol - Idol
Bristol - Idol
Andol - Idol
Bristol - Eurol
Apex - Express
Bristol - Prestol
Andol - Eurol
Express - Goeland
Rogers Distances (Molecular marker distances)
GAIumlA Distances(Traditional characteristic distances)
GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998
24
10
12
28
7
12
16
24
14
19
19
18
18
17
11
20
12
19
17
23
6
28
27
17
17
14
17
24
19
15
15
30
11
13
29
30
20
18
20
15
12
24
25
18
17
15
20
29
22
19
13
20
14
17
10
19
8
8
25
9
10
21
24
26
15
12
5
15
9
20
9
1
11
24
15
17
15
9
24
17
11
12
15
8
10
23
7
6
22
21
24
9
11
8
2
10
24
1
7
1
20
15
19
19
8
12
18
5
28
15
8
10
32
14
10
15
32
27
11
9
2
8
16
29
10
1
7
22
29
5
7
10
12
25
8
7
30
25
23
32
25
28
31
17
18
21
30
27
27
20
34
23
26
25
24
8
29
37
30
17
24
25
12
11
9
7
14
25
4
21
22
18
8
18
11
12
11
19
10
14
10
20
18
24
17
10
18
10
13
16
13
10
6
10
28
4
17
32
19
6
10
7
7
16
25
9
6
6
20
21
16
19
7
14
15
9
24
29
21
22
15
31
21
17
28
19
7
20
21
21
20
30
25
21
23
26
34
22
26
24
11
23
19
14
30
24
21
32
17
22
32
28
16
23
29
24
24
23
32
22
24
23
14
14
30
29
30
16
14
18
19
20
26
24
27
18
18
19
19
16
15
21
22
27
31
30
29
26
28
16
21
24
27
27
10
8
20
19
18
15
9
11
21
8
6
7
23
15
7
9
10
16
24
11
10
8
14
25
16
17
9
3
14
9
18
20
12
11
9
30
18
10
20
29
21
7
7
11
23
23
16
11
12
18
22
10
17
11
13
21
7
18
15
5
8
2
27
11
7
21
24
22
9
7
12
16
22
9
1
7
17
22
8
11
9
11
16
4
17
12
15
2
8
27
12
7
21
24
27
10
11
12
12
27
5
10
1
18
21
13
17
15
8
22
4
11
24
9
10
16
20
11
16
20
23
31
16
23
16
12
16
7
13
9
29
12
25
22
11
21
24
15
20
25
20
24
29
34
19
25
30
32
30
24
23
22
27
16
19
22
22
33
26
35
32
8
32
18
26
12
18
9
1
10
23
10
9
25
22
29
11
16
9
5
7
19
8
0
25
14
21
25
10
14
20
8
19
17
1
7
1
26
14
6
21
24
26
10
11
1
10
13
22
8
8
20
22
12
14
12
16
20
7
17
15
11
1
7
25
10
6
23
23
28
8
12
7
1
9
22
0
8
22
20
19
19
9
11
21
6
23
20
24
20
22
24
20
20
26
14
16
14
18
17
18
29
33
25
20
22
25
22
21
25
13
16
17
6
29
15
15
29
8
18
21
34
14
21
25
22
22
21
12
26
14
22
20
25
26
30
22
23
19
19
28
22
17
19
5
29
24
16
22
30
24
16
10
8
13
25
35
21
12
19
22
26
7
23
14
27
13
27
19
15
19
7
37
17
19
26
29
27
17
17
11
17
22
32
25
14
19
21
30
7
20
16
23
12
17
13
9
8
10
30
10
7
24
30
27
9
11
9
15
11
8
10
12
9
25
22
23
20
22
13
13
17
20
24
12
12
17
18
14
11
16
10
3
13
11
8
21
32
14
16
11
13
23
14
16
22
20
9
14
14
17
18
25
24
10
15
23
14
8
14
21
16
22
24
18
20
20
21
16
19
27
23
13
20
17
17
17
11
5
8
25
13
9
19
18
20
9
7
4
4
15
26
8
7
6
17
19
13
12
13
9
17

Graph99

169
677
61 20 varieacuteteacutes preacutesentes
93 19 varieacuteteacutes preacutesentes
Goeland-Lady
Goeland-Apex
Envol-Idol
Apex-Lady
Ceres-Falcon
Bristol-Eurol
Apex-Envol
Andol-Eurol
Eurol-Idol
Bristol-Prestol
Doublol-Duetol
Capitol-Vivol
Eurol-Samourai
Bristol-Samourai
Akamar-Duetol
Contact-Envol
Duetol-Navajo
Apex-Contact
Prestol-Samouraiet Goeland-Idol
Andol-Zorro
Falcon-Navajo
Andol-Falcon
Falcon-Goeland
Lady-Navajo
Duetol-Lady
Express-Zorro
Contact-Goeland
Duetol-Lady
Distances de ROGERS
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19981999
1
1
1
1
0
0
11
11
1
1
2
2
13
13
5
5
8
8
8
8
6
6
5
5
3
7
3
7
8
10
8
10
8
8
5
5
15
15
15
15
6
6
6
6
5
5
13
13
1
1
14
14
18
18
3
3
2
2
3
3
15
15
12
12
13
13
8
8
12
18
12
18
17
17
20
20
12
12
24
2
24
2
5
20
2
20
5
11
2
11
13
13
1
1
20
20
3
16
3
16
15
15
2
8
8
2
8
8
11
11
12
12
15
15
2
4
4
2
8
13
8
13
17
19
19
17
13
13
11
14
11
14
13
13
12
17
8
12
7
20
20
7
17
8
8
8
11
11
9
9
9
9
12
18
12
18
8
8
15
15
15
15
10
10
5
5
8
8
20
6
6
20
13
13
2
15
15
2
6
6
10
10
16
16
14
6
14
6
15
21
15
21
7
7
21
21
20
7
7
20
15
15
7
7
14
6
6
9
2
2
14
9
10
10
16
16
15
15
13
6
13
6
11
11
19
8
10
10
22
8
22
19
5
5
4
5
4
5
5
7
5
15
5
12
15
12
7
5
0
12
6
0
6
12
22
11
14
14
13
22
11
13
10
6
20
6
20
10
4
15
1
4
1
8
11
15
8
11
18
18
13
15
13
15
13
13
5
17
5
9
3
3
15
12
15
5
12
5
17
9
12
12
14
14
8
10
13
10
13
8
23
6
12
9
6
9
12
23
14
12
10
12
14
10
14
14
16
12
13
12
10
13
10
16
12
15
13
12
9
10
9
5
15
13
2
5
2
13
10
13
4
9
17
9
4
17
10
12
9
10
12
9
16
8
8
12
8
16
12
8
13
13
6
3
6
3
11
12
3
20
12
20
14
11
3
14
18
7
7
18
19
19
13
19
19
11
13
11
3
18
2
20
28
3
20
2
28
12
18
12
5
13
8
12
13
5
8
8
12
8
13
9
13
12
9
16
12
18
18
16
10
10
16
15
16
15
14
11
14
11
10
16
8
16
10
8
10
2
8
12
2
6
11
23
10
6
19
8
11
23
12
19
6
6
10
7
8
1
9
21
9
21
7
1
10
8
18
14
18
14
17
17
8
8
9
5
14
3
9
21
5
16
13
14
16
13
21
3
10
5
5
10
13
13
4
15
4
15
5
8
8
8
5
8
5
5
17
15
17
15
10
8
7
8
10
9
20
20
7
9
15
12
10
15
18
12
18
10
15
23
15
23
12
12
19
19
14
14
14
11
11
8
14
8
17
6
17
6
13
13
10
2
2
5
18
10
5
18
10
7
10
13
15
13
15
15
13
15
13
7
2
2
8
8
14
7
10
14
7
10
11
14
11
18
14
18
13
13
16
16
15
16
16
15
9
2
11
2
9
12
12
11
12
15
12
15
8
11
12
14
14
8
12
11
11
24
11
24
13
13
13
15
16
16
22
13
15
16
22
13
15
15
16
14
13
12
14
12
10
10
15
10
6
10
15
6
9
9
13
13
10
11
11
10
14
14
10
10
17
8
7
8
8
17
8
7
8
8
12
2
2
12
17
17
12
12
18
18
18
18
5
8
5
16
16
8
14
14
14
8
22
22
13
13
14
14
9
14
9
8
13
13
11
13
11
13
9
9
12
12
9
9
13
13
10
10
13
13
6
6
11
11
17
17
9
9
15
15
9
9
6
6
12
12
24
24
8
8
12
19
12
19
9
9
12
12

Model 1 Characteristic-specific molecular markers

Example 1 gene specific marker for herbicide tolerance introduced by genetic modification On the basis that [hellip] - there is verification of the reliability of the link between the marker and the characteristic - different markers for the same characteristic are different methods for examining the same characteristic [hellip]

Fusarium race 0 ex 1 Example Characteristic-specific molecular marker with incomplete information on state of expression

Model 1 Characteristic-specific molecular markers

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

ex Maize parent lines

Model 2 Combining phenotypic and molecular distances in the management of variety collections

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

2

02

Model 2 Combining phenotypic and molecular distances in the management of variety collections (continued)

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

13

New Model 3 Genetic selection of similar varieties for the first growing cycle

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
0038
0038
0042
0042
0045
0045
0063
0063
0067
0067
0068
0068
0075
0075
0106
0106
011
011
0114
0114
0115
0115
0116
0116
0123
0123
0123
0123
0131
0131
0131
0131
0132
0132
0145
0145
0149
0149
0149
0149
017
017
0176
0176
018
018
0185
0185
0186
0186
0194
0194
0198
0198
0199
0199
02
02
0201
0201
0202
0202
0203
0203
0204
0204
0207
0207
021
021
021
021
0213
0213
0214
0214
0217
0217
0217
0217
0217
0217
0219
0219
0219
0219
0219
0219
0219
0219
022
022
0224
0224
0225
0225
0225
0225
0225
0225
0226
0226
0227
0227
0227
0227
0227
0227
0228
0228
0228
0228
0228
0228
023
023
023
023
023
023
023
023
0231
0231
0231
0231
0232
0232
0232
0232
0232
0232
0233
0233
0235
0235
0235
0235
0235
0235
0235
0235
0235
0235
0236
0236
0236
0236
0238
0238
0238
0238
0239
0239
0239
0239
0241
0241
0242
0242
0244
0244
0245
0245
0246
0246
0247
0247
0249
0249
0249
0249
025
025
0251
0251
0251
0251
0252
0252
0252
0252
0252
0252
0253
0253
0253
0253
0254
0254
0254
0254
0255
0255
0259
0259
0259
0259
0259
0259
026
026
026
026
0262
0262
0262
0262
0262
0262
0262
0262
0264
0264
0265
0265
0266
0266
0267
0267
0267
0267
0268
0268
0269
0269
0269
0269
0269
0269
0269
0269
027
027
027
027
027
027
0272
0272
0272
0272
0272
0272
0272
0272
0272
0272
0273
0273
0273
0273
0273
0273
0275
0275
0275
0275
0275
0275
0275
0275
0276
0276
0276
0276
0276
0276
0278
0278
0278
0278
0278
0278
0278
0278
0278
0278
0279
0279
0279
0279
0279
0279
028
028
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0282
0282
0282
0282
0283
0283
0283
0283
0283
0283
0284
0284
0284
0284
0284
0284
0284
0284
0285
0285
0285
0285
0285
0285
0285
0285
0286
0286
0286
0286
0286
0286
0286
0286
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0288
0288
0288
0288
0288
0288
0289
0289
0289
0289
0289
0289
029
029
029
029
029
029
029
029
0291
0291
0292
0292
0292
0292
0293
0293
0293
0293
0293
0293
0293
0293
0293
0293
0294
0294
0294
0294
0294
0294
0295
0295
0295
0295
0295
0295
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0298
0298
0298
0298
0298
0298
0298
0298
0298
0298
0299
0299
0299
0299
0299
0299
0299
0299
0299
0299
03
03
0302
0302
0302
0302
0303
0303
0303
0303
0304
0304
0304
0304
0304
0304
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0307
0307
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
031
031
031
031
031
031
0311
0311
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
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0238
0272
0254
0228
0145
0268
0322
0361
025
0259
0275
029
0299
0185
0312
0323
0287
0312
0326
0307
0284
0304
0284
0123
0262
0186
0405
0319
0329
032
0286
0322
0316
011
0252
0309
0309
0249
0106
0276
0322
0412
0115
0219
0329
0276
0372
0319
0311
0329
0293
0241
0278
0422
0333
0287
0341
0324
0361
0352
0288
0252
0327
0293
023
0281
0253
0398
0389
0282
0284
0272
0342
0405
0312
0273
0149
0336
027
0278
0345
0068
0299
0285
0312
025
0284
0294
0309
0327
0231
0262
0287
0296
0269
0358
0342
029
0265
0304
0335
0287
0245
0239
034
0213
0312
0351
0225
0235
0217
0253
0259
0293
0281
0309
0293
0231
0198
0296
0219
0296
0299
0342
0306
0207
032
0345
021
027
028
0272
0038
0227
0358
0281
0236
0202
0246
0275
0288
0217
0249
023
0262
0198
0201
0045
0319
0313
0244
0236
0204
0285
0321
0304
0289
0289
0123
0219
0114
0348
0306
0346
0294
0296
029
0306
0042
0106
0281
0287
0296
0201
0225
0315
038
0063
0194
0367
0225
0364
0352
0292
0298
0287
0067
0247
0351
0309
0269
0232
0281
0299
0315
023
0276
0253
0296
0219
0045
0225
0299
0327
0251
0232
0228
031
0335
0324
0295
0252
0333
0327
0306
0275
0275
0302
0254
0302
0185
0231
0288
0322
0398
0269
0296
0319
0315
0299
0361
0339
0294
0318
0354
0316
0321
0292
0342
0404
0298
0383
0351
0327
033
0331
0306
0312
0309
0377
0412
0389
0358
0299
0313
038
0327
0361
0395
0368
0272
0389
0332
0336
0312
0312
0132
0242
0116
0364
0357
0376
034
0342
0323
032
0075
0115
0282
0342
0342
0244
0063
0251
0339
0395
0199
0361
0232
0338
037
0318
0282
0235
0233
02
0359
0281
0342
0285
029
0287
0306
021
0219
0284
029
0306
0236
0194
0232
0294
0368
0199
0342
0235
0338
0303
023
0267
038
0203
0346
0364
0278
0176
0214
0281
0312
029
0345
0329
0272
0265
0207
0204
0367
0228
0318
0272
0361
0342
0358
0345
0287
0283
0325
0266
0293
0259
0361
031
0361
0356
0339
0326
0339
0239
0276
0342
0304
032
0285
0225
031
0354
0389
0232
0235
0358
0342
0373
0296
0287
0386
0329
0329
018
0335
0361
0286
0339
0307
032
0334
0372
0405
0335
0345
0321
0364
0335
0316
0332
0338
0338
0345
0342
031
0295
0252
0352
0298
0336
0342
0269
0235
0279
0235
0284
0287
0329
0319
0312
0287
021
0304
0352
0324
0321
0336
037
0303
0287
0373
031
Page 7: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

Document reference Title

TGP15 Guidance on the Use of Biochemical and Molecular Markers in the Examination of Distinctness Uniformity and Stability (DUS)

UPOVINF181 Possible Use of Molecular Markers in the Examination of Distinctness Uniformity and Stability (2011)

Document reference Title

UPOVINF171 Guidelines for DNA Profiling Molecular Marker Selection and Database Construction (ldquoBMT Guidelinesrdquo) (2010)

STATUS OF UPOV DOCUMENTS CONCERNING MOLECULAR TECHNIQUES

Calibrated molecular distances in the management of variety collections

8

02468

10121416182022242628303234363840

0 005 01 015 02 025 03 035 04 045

GA

IumlA D

ista

nces

(Tra

ditio

nal c

hara

cter

istic

di

stan

ces)

Rogers Distances (Molecular marker distances)

GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998

Type 3 Type 1

Type 4Type 2Express - Lady

Duetol - TapidorEurol - Zorro

Ceres - Cheyenne

Express - Zorro

Zorro - Apex

Goeland - Lady

Apex - GoelandApex - Lady

Eurol - Idol

Bristol - Idol

Andol - IdolBristol - Eurol Apex - Express

Bristol - Prestol Andol - Eurol Express - Goeland

Distinctness plus (Traditional characteristics)

Distinctness plus (Molecular markers)

Type 1 Yes Yes Type 2 No No Type 3 Yes No Type 4 No Yes

Graph96

Express-Falcon
Falcon-Lady
Bristol-Falcon
Doublol-Cheyenne
Akamar-Apex
Apex-Goeland
Envol-Idol
Goeland-Lady
Apex-Lady
Bristol-Idol
Eurol-Prestol
Andol-Envol
Bristol-Envol
Idol-Goeland
Apex-Bristol
Apex-Idol
Bristol--Lady
Bristol-Goeland
34 9 varieacuteteacutes preacutesentes
193
759
14 8 varieacuteteacutes preacutesentes
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19951996
23
14
3
14
11
10
20
16
9
24
11
18
21
19
12
22
6
13
6
27
10
32
24
14
20
19
16
23
14
19
19
22
21
14
22
19
13
11
16
21
18
19
11
19
17
19
22
26
25
17
19
21
13
24
14
14
14
9
20
9
11
22
14
21
16
5
10
8
10
16
14
6
16
29
20
14
16
16
23
19
18
3
19
14
4
18
11
16
14
12
19
12
8
13
11
6
16
0
5
1
26
8
19
20
10
20
20
9
14
19
9
4
20
13
15
19
11
16
11
2
11
7
5
16
4
1
5
23
19
18
13
16
16
17
10
11
22
20
18
20
18
13
10
13
23
17
22
24
24
20
25
18
20
18
14
13
23
18
20
18
11
20
10
21
9
11
13
18
10
19
19
17
8
16
14
13
14
20
11
12
14
28
16
24
26
14
19
17
23
20
14
11
16
15
13
10
20
14
3
10
10
17
20
15
14
15
15
19
20
11
19
19
17
14
14
22
16
22
22
14
19
10
19
20
10
20
16
20
20
19
18
25
14
19
12
16
16
17
10
26
8
14
13
11
11
16
12
11
17
8
10
16
19
13
13
18
22
21
12
12
10
15
23
16
26
16
22
14
11
26
18
16
5
8
2
22
16
10
20
11
14
13
13
8
9
15
6
1
11
24
18
15
12
17
17
18
13
9
19
14
12
11
13
19
14
10
18
19
11
24
16
14
21
12
8
14
19
12
26
13
22
21
17
11
24
13
21
19
16
23
17
3
20
18
13
14
21
25
24
12
19
15
22
16
16
23
17
19
15
17
25
21
21
10
13
11
24
14
17
20
24
21
18
13
8
8
18
11
14
12
26
23
3
20
14
20
20
16
19
18
8
11
7
24
13
20
19
16
25
22
8
8
4
15
11
9
8
31
19
13
14
10
24
23
10
12
19
10
6
5
20
14
15
18
14
24
21
9
8
4
16
6
8
5
29
12
10
14
7
26
23
7
11
12
12
14
15
15
16
16
16
16
16
16
16
16
18
20
21
21
21
22
22
22
23
24
25
25
27
6
19
14
0
4
18
11
15
14
12
19
12
6
11
11
6
16
5
1
26
11
18
18
13
17
20
9
13
17
6
5
1
20
12
15
19
8
15
10
1
14
9
8
16
5
8
23
20
20
13
19
16
17
12
6
19
16
1
5
18
14
19
12
14
22
15
11
12
8
5
16
1
8
26
9
16
20
9
20
20
6
27
22
29
26
23
14
28
20
16
19
16
23
24
26
31
29
27
26
23
26
25
26
19
33
19
12
27
10
26
20
8
19
13
16
11
16
12
16
16
18
23
19
12
23
11
20
9
25
24
22
13
16
25
14
32
25
14
19
18
23
24
19
17
26
23
26
15
3
13
10
22
18
20
16
26
24
14
19
18
24
20
24
17
16
20
13
18
26
19
10
13
17
16
12
20
14
14
22
18
13
20
19
22
14
28
9
21
13
14
19
16
10
16
20
14
17
26
22
19
22
17
14
10
7
16
13
19
9
33
13
19
28
25
22
15
20
21
23
20
16
18
19
14
8
21
15
14
17
20
24
26
24
17
16
20
19
16
18
9
25
13
20
19
13
19
20
17
11
17
14
14
17
17
11
18
20
23
23
21
20
17
20
12
25
24
21
22
13
25
16
24
18
9
10
20
23
22
13
11
25
26
13
16
10
7
21
9
12
6
27
14
20
13
15
20
25

Graph97

Apex-Goeland
Eurol-Prestol
Eurol-Andol
Apex-Envol
Apex-Express
Envol-Lady
Akamar-Doublol
Contact-Navajo
Ceres-Goeland
Ceres-Lady
Apex-Ceres
Columbus-Tapidor
Goeland-Lady
Apex-Lady
212
16 8 varieacuteteacutes preacutesentes
16 10 varieacuteteacutes preacutesentes
756
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19961997
18
13
13
18
9
17
24
9
6
4
8
13
15
13
9
10
8
13
10
20
8
15
16
20
6
12
13
18
13
19
14
19
19
15
24
24
20
17
22
17
18
22
17
20
17
17
12
18
18
21
11
20
7
24
13
13
15
18
21
20
18
22
18
18
19
9
3
12
18
15
18
13
9
15
18
9
19
18
18
13
19
13
19
15
19
15
4
12
15
12
16
16
5
14
2
8
8
3
17
6
25
10
16
23
10
15
15
8
18
14
18
19
19
17
15
17
13
22
13
21
16
18
19
21
10
9
11
15
17
16
15
26
14
10
19
9
19
21
15
19
25
26
15
13
9
9
22
23
16
11
16
15
19
17
23
14
21
17
18
11
17
15
17
19
20
4
17
25
10
21
11
15
26
12
10
12
10
11
5
16
5
24
18
17
24
20
18
15
21
24
15
18
12
15
26
10
18
20
22
23
21
13
15
20
13
10
16
12
15
20
13
18
11
17
12
18
9
24
22
15
17
15
21
18
14
12
15
23
22
15
22
17
9
20
20
16
11
18
12
24
5
19
16
6
24
18
12
13
13
11
20
14
7
8
13
14
18
6
11
10
8
8
14
3
16
15
21
12
13
20
4
20
18
16
22
9
15
22
12
7
10
19
18
20
9
10
16
17
15
14
10
18
16
23
10
15
22
8
17
19
16
13
9
26
23
15
8
10
17
23
19
12
22
19
9
14
13
9
23
12
25
15
13
18
13
22
9
5
21
22
12
21
23
13
19
17
11
10
12
11
8
10
1
20
12
16
24
21
19
19
21
15
17
3
14
16
23
10
13
22
14
18
23
11
16
13
12
14
10
8
17
11
2
19
20
20
17
26
13
18
12
2
18
16
12
15
15
18
20
19
10
16
15
15
8
18
11
29
9
18
22
8
18
19
6
9
22
18
8
19
11
10
20
22
6
9
12
12
13
15
13
11
11
7
22
7
16
22
18
19
15
22
10
17
15
8
21
16
11
13
17
11
10
22
11
12
15
13
8
17
11
23
11
13
19
20
16
19
22
8
20
18
3
10
15
5
10
9
10
16
19
8
14
8
11
8
15
9
23
11
15
21
19
12
12
10
13
17
13
17
9
19
16
16
20
8
17
9
10
10
18
11
17
15
8
10
8
11
10
24
19
15
23
10
17
9
6
11
17
5
12
20
8
15
14
1
8
11
7
11
9
8
17
13
17
20
21
15
10
16
20
12
15
25
15
23
24
15
16
14
14
13
20
17
29
22
23
23
10
17
15
17
14
23
19
13
30
8
18
18
10
17
14
18
20
11
3
10
9
12
11
9
7
11
11
8
13
15
6
10
15
15
20
19
15
18
9
16
16
21
17
13
18
16
18
23
16
2
18
16
13
15
11
17
17
6
13
20
18
20
25
16
21
19
23
15
17
24
18
12
15
16
12
24
19
22
22
19
21
10
20
14
10
13
26
9
24
21
20
11
18
10
26
18
20
11
24
21
23
25
21
20
8
18
20
19
24
21
23
15
20
26
26
15
14
6
20
18
15
14
11
18
17
5
12
10
15
19
20
18
19
16
12
19
15
19
15
18
9
26
16
14
12
7
13
15
10
17
15
12
19
13
15
13
19
17
19
15
19
12
15
10
13
20
20
24
15
16
21
13
24
19
8
19
15
21
18
16
20
22
18
21
26
6
22
22
10
23
16
30
19
25
21
14
14
21

Graph98

Type 3
Type 1
Type 4
Type 2
Express - Lady
Duetol - Tapidor
Eurol - Zorro
Ceres - Cheyenne
Express - Zorro
Zorro - Apex
Goeland - Lady
Apex - Goeland
Apex - Lady
Eurol - Idol
Bristol - Idol
Andol - Idol
Bristol - Eurol
Apex - Express
Bristol - Prestol
Andol - Eurol
Express - Goeland
Rogers Distances (Molecular marker distances)
GAIumlA Distances(Traditional characteristic distances)
GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998
24
10
12
28
7
12
16
24
14
19
19
18
18
17
11
20
12
19
17
23
6
28
27
17
17
14
17
24
19
15
15
30
11
13
29
30
20
18
20
15
12
24
25
18
17
15
20
29
22
19
13
20
14
17
10
19
8
8
25
9
10
21
24
26
15
12
5
15
9
20
9
1
11
24
15
17
15
9
24
17
11
12
15
8
10
23
7
6
22
21
24
9
11
8
2
10
24
1
7
1
20
15
19
19
8
12
18
5
28
15
8
10
32
14
10
15
32
27
11
9
2
8
16
29
10
1
7
22
29
5
7
10
12
25
8
7
30
25
23
32
25
28
31
17
18
21
30
27
27
20
34
23
26
25
24
8
29
37
30
17
24
25
12
11
9
7
14
25
4
21
22
18
8
18
11
12
11
19
10
14
10
20
18
24
17
10
18
10
13
16
13
10
6
10
28
4
17
32
19
6
10
7
7
16
25
9
6
6
20
21
16
19
7
14
15
9
24
29
21
22
15
31
21
17
28
19
7
20
21
21
20
30
25
21
23
26
34
22
26
24
11
23
19
14
30
24
21
32
17
22
32
28
16
23
29
24
24
23
32
22
24
23
14
14
30
29
30
16
14
18
19
20
26
24
27
18
18
19
19
16
15
21
22
27
31
30
29
26
28
16
21
24
27
27
10
8
20
19
18
15
9
11
21
8
6
7
23
15
7
9
10
16
24
11
10
8
14
25
16
17
9
3
14
9
18
20
12
11
9
30
18
10
20
29
21
7
7
11
23
23
16
11
12
18
22
10
17
11
13
21
7
18
15
5
8
2
27
11
7
21
24
22
9
7
12
16
22
9
1
7
17
22
8
11
9
11
16
4
17
12
15
2
8
27
12
7
21
24
27
10
11
12
12
27
5
10
1
18
21
13
17
15
8
22
4
11
24
9
10
16
20
11
16
20
23
31
16
23
16
12
16
7
13
9
29
12
25
22
11
21
24
15
20
25
20
24
29
34
19
25
30
32
30
24
23
22
27
16
19
22
22
33
26
35
32
8
32
18
26
12
18
9
1
10
23
10
9
25
22
29
11
16
9
5
7
19
8
0
25
14
21
25
10
14
20
8
19
17
1
7
1
26
14
6
21
24
26
10
11
1
10
13
22
8
8
20
22
12
14
12
16
20
7
17
15
11
1
7
25
10
6
23
23
28
8
12
7
1
9
22
0
8
22
20
19
19
9
11
21
6
23
20
24
20
22
24
20
20
26
14
16
14
18
17
18
29
33
25
20
22
25
22
21
25
13
16
17
6
29
15
15
29
8
18
21
34
14
21
25
22
22
21
12
26
14
22
20
25
26
30
22
23
19
19
28
22
17
19
5
29
24
16
22
30
24
16
10
8
13
25
35
21
12
19
22
26
7
23
14
27
13
27
19
15
19
7
37
17
19
26
29
27
17
17
11
17
22
32
25
14
19
21
30
7
20
16
23
12
17
13
9
8
10
30
10
7
24
30
27
9
11
9
15
11
8
10
12
9
25
22
23
20
22
13
13
17
20
24
12
12
17
18
14
11
16
10
3
13
11
8
21
32
14
16
11
13
23
14
16
22
20
9
14
14
17
18
25
24
10
15
23
14
8
14
21
16
22
24
18
20
20
21
16
19
27
23
13
20
17
17
17
11
5
8
25
13
9
19
18
20
9
7
4
4
15
26
8
7
6
17
19
13
12
13
9
17

Graph99

169
677
61 20 varieacuteteacutes preacutesentes
93 19 varieacuteteacutes preacutesentes
Goeland-Lady
Goeland-Apex
Envol-Idol
Apex-Lady
Ceres-Falcon
Bristol-Eurol
Apex-Envol
Andol-Eurol
Eurol-Idol
Bristol-Prestol
Doublol-Duetol
Capitol-Vivol
Eurol-Samourai
Bristol-Samourai
Akamar-Duetol
Contact-Envol
Duetol-Navajo
Apex-Contact
Prestol-Samouraiet Goeland-Idol
Andol-Zorro
Falcon-Navajo
Andol-Falcon
Falcon-Goeland
Lady-Navajo
Duetol-Lady
Express-Zorro
Contact-Goeland
Duetol-Lady
Distances de ROGERS
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19981999
1
1
1
1
0
0
11
11
1
1
2
2
13
13
5
5
8
8
8
8
6
6
5
5
3
7
3
7
8
10
8
10
8
8
5
5
15
15
15
15
6
6
6
6
5
5
13
13
1
1
14
14
18
18
3
3
2
2
3
3
15
15
12
12
13
13
8
8
12
18
12
18
17
17
20
20
12
12
24
2
24
2
5
20
2
20
5
11
2
11
13
13
1
1
20
20
3
16
3
16
15
15
2
8
8
2
8
8
11
11
12
12
15
15
2
4
4
2
8
13
8
13
17
19
19
17
13
13
11
14
11
14
13
13
12
17
8
12
7
20
20
7
17
8
8
8
11
11
9
9
9
9
12
18
12
18
8
8
15
15
15
15
10
10
5
5
8
8
20
6
6
20
13
13
2
15
15
2
6
6
10
10
16
16
14
6
14
6
15
21
15
21
7
7
21
21
20
7
7
20
15
15
7
7
14
6
6
9
2
2
14
9
10
10
16
16
15
15
13
6
13
6
11
11
19
8
10
10
22
8
22
19
5
5
4
5
4
5
5
7
5
15
5
12
15
12
7
5
0
12
6
0
6
12
22
11
14
14
13
22
11
13
10
6
20
6
20
10
4
15
1
4
1
8
11
15
8
11
18
18
13
15
13
15
13
13
5
17
5
9
3
3
15
12
15
5
12
5
17
9
12
12
14
14
8
10
13
10
13
8
23
6
12
9
6
9
12
23
14
12
10
12
14
10
14
14
16
12
13
12
10
13
10
16
12
15
13
12
9
10
9
5
15
13
2
5
2
13
10
13
4
9
17
9
4
17
10
12
9
10
12
9
16
8
8
12
8
16
12
8
13
13
6
3
6
3
11
12
3
20
12
20
14
11
3
14
18
7
7
18
19
19
13
19
19
11
13
11
3
18
2
20
28
3
20
2
28
12
18
12
5
13
8
12
13
5
8
8
12
8
13
9
13
12
9
16
12
18
18
16
10
10
16
15
16
15
14
11
14
11
10
16
8
16
10
8
10
2
8
12
2
6
11
23
10
6
19
8
11
23
12
19
6
6
10
7
8
1
9
21
9
21
7
1
10
8
18
14
18
14
17
17
8
8
9
5
14
3
9
21
5
16
13
14
16
13
21
3
10
5
5
10
13
13
4
15
4
15
5
8
8
8
5
8
5
5
17
15
17
15
10
8
7
8
10
9
20
20
7
9
15
12
10
15
18
12
18
10
15
23
15
23
12
12
19
19
14
14
14
11
11
8
14
8
17
6
17
6
13
13
10
2
2
5
18
10
5
18
10
7
10
13
15
13
15
15
13
15
13
7
2
2
8
8
14
7
10
14
7
10
11
14
11
18
14
18
13
13
16
16
15
16
16
15
9
2
11
2
9
12
12
11
12
15
12
15
8
11
12
14
14
8
12
11
11
24
11
24
13
13
13
15
16
16
22
13
15
16
22
13
15
15
16
14
13
12
14
12
10
10
15
10
6
10
15
6
9
9
13
13
10
11
11
10
14
14
10
10
17
8
7
8
8
17
8
7
8
8
12
2
2
12
17
17
12
12
18
18
18
18
5
8
5
16
16
8
14
14
14
8
22
22
13
13
14
14
9
14
9
8
13
13
11
13
11
13
9
9
12
12
9
9
13
13
10
10
13
13
6
6
11
11
17
17
9
9
15
15
9
9
6
6
12
12
24
24
8
8
12
19
12
19
9
9
12
12

Model 1 Characteristic-specific molecular markers

Example 1 gene specific marker for herbicide tolerance introduced by genetic modification On the basis that [hellip] - there is verification of the reliability of the link between the marker and the characteristic - different markers for the same characteristic are different methods for examining the same characteristic [hellip]

Fusarium race 0 ex 1 Example Characteristic-specific molecular marker with incomplete information on state of expression

Model 1 Characteristic-specific molecular markers

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

ex Maize parent lines

Model 2 Combining phenotypic and molecular distances in the management of variety collections

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

2

02

Model 2 Combining phenotypic and molecular distances in the management of variety collections (continued)

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

13

New Model 3 Genetic selection of similar varieties for the first growing cycle

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
0038
0038
0042
0042
0045
0045
0063
0063
0067
0067
0068
0068
0075
0075
0106
0106
011
011
0114
0114
0115
0115
0116
0116
0123
0123
0123
0123
0131
0131
0131
0131
0132
0132
0145
0145
0149
0149
0149
0149
017
017
0176
0176
018
018
0185
0185
0186
0186
0194
0194
0198
0198
0199
0199
02
02
0201
0201
0202
0202
0203
0203
0204
0204
0207
0207
021
021
021
021
0213
0213
0214
0214
0217
0217
0217
0217
0217
0217
0219
0219
0219
0219
0219
0219
0219
0219
022
022
0224
0224
0225
0225
0225
0225
0225
0225
0226
0226
0227
0227
0227
0227
0227
0227
0228
0228
0228
0228
0228
0228
023
023
023
023
023
023
023
023
0231
0231
0231
0231
0232
0232
0232
0232
0232
0232
0233
0233
0235
0235
0235
0235
0235
0235
0235
0235
0235
0235
0236
0236
0236
0236
0238
0238
0238
0238
0239
0239
0239
0239
0241
0241
0242
0242
0244
0244
0245
0245
0246
0246
0247
0247
0249
0249
0249
0249
025
025
0251
0251
0251
0251
0252
0252
0252
0252
0252
0252
0253
0253
0253
0253
0254
0254
0254
0254
0255
0255
0259
0259
0259
0259
0259
0259
026
026
026
026
0262
0262
0262
0262
0262
0262
0262
0262
0264
0264
0265
0265
0266
0266
0267
0267
0267
0267
0268
0268
0269
0269
0269
0269
0269
0269
0269
0269
027
027
027
027
027
027
0272
0272
0272
0272
0272
0272
0272
0272
0272
0272
0273
0273
0273
0273
0273
0273
0275
0275
0275
0275
0275
0275
0275
0275
0276
0276
0276
0276
0276
0276
0278
0278
0278
0278
0278
0278
0278
0278
0278
0278
0279
0279
0279
0279
0279
0279
028
028
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0282
0282
0282
0282
0283
0283
0283
0283
0283
0283
0284
0284
0284
0284
0284
0284
0284
0284
0285
0285
0285
0285
0285
0285
0285
0285
0286
0286
0286
0286
0286
0286
0286
0286
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0288
0288
0288
0288
0288
0288
0289
0289
0289
0289
0289
0289
029
029
029
029
029
029
029
029
0291
0291
0292
0292
0292
0292
0293
0293
0293
0293
0293
0293
0293
0293
0293
0293
0294
0294
0294
0294
0294
0294
0295
0295
0295
0295
0295
0295
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0298
0298
0298
0298
0298
0298
0298
0298
0298
0298
0299
0299
0299
0299
0299
0299
0299
0299
0299
0299
03
03
0302
0302
0302
0302
0303
0303
0303
0303
0304
0304
0304
0304
0304
0304
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0307
0307
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
031
031
031
031
031
031
0311
0311
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0313
0313
0313
0313
0314
0314
0315
0315
0315
0315
0316
0316
0316
0316
0316
0316
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0307
0284
0304
0284
0123
0262
0186
0405
0319
0329
032
0286
0322
0316
011
0252
0309
0309
0249
0106
0276
0322
0412
0115
0219
0329
0276
0372
0319
0311
0329
0293
0241
0278
0422
0333
0287
0341
0324
0361
0352
0288
0252
0327
0293
023
0281
0253
0398
0389
0282
0284
0272
0342
0405
0312
0273
0149
0336
027
0278
0345
0068
0299
0285
0312
025
0284
0294
0309
0327
0231
0262
0287
0296
0269
0358
0342
029
0265
0304
0335
0287
0245
0239
034
0213
0312
0351
0225
0235
0217
0253
0259
0293
0281
0309
0293
0231
0198
0296
0219
0296
0299
0342
0306
0207
032
0345
021
027
028
0272
0038
0227
0358
0281
0236
0202
0246
0275
0288
0217
0249
023
0262
0198
0201
0045
0319
0313
0244
0236
0204
0285
0321
0304
0289
0289
0123
0219
0114
0348
0306
0346
0294
0296
029
0306
0042
0106
0281
0287
0296
0201
0225
0315
038
0063
0194
0367
0225
0364
0352
0292
0298
0287
0067
0247
0351
0309
0269
0232
0281
0299
0315
023
0276
0253
0296
0219
0045
0225
0299
0327
0251
0232
0228
031
0335
0324
0295
0252
0333
0327
0306
0275
0275
0302
0254
0302
0185
0231
0288
0322
0398
0269
0296
0319
0315
0299
0361
0339
0294
0318
0354
0316
0321
0292
0342
0404
0298
0383
0351
0327
033
0331
0306
0312
0309
0377
0412
0389
0358
0299
0313
038
0327
0361
0395
0368
0272
0389
0332
0336
0312
0312
0132
0242
0116
0364
0357
0376
034
0342
0323
032
0075
0115
0282
0342
0342
0244
0063
0251
0339
0395
0199
0361
0232
0338
037
0318
0282
0235
0233
02
0359
0281
0342
0285
029
0287
0306
021
0219
0284
029
0306
0236
0194
0232
0294
0368
0199
0342
0235
0338
0303
023
0267
038
0203
0346
0364
0278
0176
0214
0281
0312
029
0345
0329
0272
0265
0207
0204
0367
0228
0318
0272
0361
0342
0358
0345
0287
0283
0325
0266
0293
0259
0361
031
0361
0356
0339
0326
0339
0239
0276
0342
0304
032
0285
0225
031
0354
0389
0232
0235
0358
0342
0373
0296
0287
0386
0329
0329
018
0335
0361
0286
0339
0307
032
0334
0372
0405
0335
0345
0321
0364
0335
0316
0332
0338
0338
0345
0342
031
0295
0252
0352
0298
0336
0342
0269
0235
0279
0235
0284
0287
0329
0319
0312
0287
021
0304
0352
0324
0321
0336
037
0303
0287
0373
031
Page 8: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

Calibrated molecular distances in the management of variety collections

8

02468

10121416182022242628303234363840

0 005 01 015 02 025 03 035 04 045

GA

IumlA D

ista

nces

(Tra

ditio

nal c

hara

cter

istic

di

stan

ces)

Rogers Distances (Molecular marker distances)

GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998

Type 3 Type 1

Type 4Type 2Express - Lady

Duetol - TapidorEurol - Zorro

Ceres - Cheyenne

Express - Zorro

Zorro - Apex

Goeland - Lady

Apex - GoelandApex - Lady

Eurol - Idol

Bristol - Idol

Andol - IdolBristol - Eurol Apex - Express

Bristol - Prestol Andol - Eurol Express - Goeland

Distinctness plus (Traditional characteristics)

Distinctness plus (Molecular markers)

Type 1 Yes Yes Type 2 No No Type 3 Yes No Type 4 No Yes

Graph96

Express-Falcon
Falcon-Lady
Bristol-Falcon
Doublol-Cheyenne
Akamar-Apex
Apex-Goeland
Envol-Idol
Goeland-Lady
Apex-Lady
Bristol-Idol
Eurol-Prestol
Andol-Envol
Bristol-Envol
Idol-Goeland
Apex-Bristol
Apex-Idol
Bristol--Lady
Bristol-Goeland
34 9 varieacuteteacutes preacutesentes
193
759
14 8 varieacuteteacutes preacutesentes
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19951996
23
14
3
14
11
10
20
16
9
24
11
18
21
19
12
22
6
13
6
27
10
32
24
14
20
19
16
23
14
19
19
22
21
14
22
19
13
11
16
21
18
19
11
19
17
19
22
26
25
17
19
21
13
24
14
14
14
9
20
9
11
22
14
21
16
5
10
8
10
16
14
6
16
29
20
14
16
16
23
19
18
3
19
14
4
18
11
16
14
12
19
12
8
13
11
6
16
0
5
1
26
8
19
20
10
20
20
9
14
19
9
4
20
13
15
19
11
16
11
2
11
7
5
16
4
1
5
23
19
18
13
16
16
17
10
11
22
20
18
20
18
13
10
13
23
17
22
24
24
20
25
18
20
18
14
13
23
18
20
18
11
20
10
21
9
11
13
18
10
19
19
17
8
16
14
13
14
20
11
12
14
28
16
24
26
14
19
17
23
20
14
11
16
15
13
10
20
14
3
10
10
17
20
15
14
15
15
19
20
11
19
19
17
14
14
22
16
22
22
14
19
10
19
20
10
20
16
20
20
19
18
25
14
19
12
16
16
17
10
26
8
14
13
11
11
16
12
11
17
8
10
16
19
13
13
18
22
21
12
12
10
15
23
16
26
16
22
14
11
26
18
16
5
8
2
22
16
10
20
11
14
13
13
8
9
15
6
1
11
24
18
15
12
17
17
18
13
9
19
14
12
11
13
19
14
10
18
19
11
24
16
14
21
12
8
14
19
12
26
13
22
21
17
11
24
13
21
19
16
23
17
3
20
18
13
14
21
25
24
12
19
15
22
16
16
23
17
19
15
17
25
21
21
10
13
11
24
14
17
20
24
21
18
13
8
8
18
11
14
12
26
23
3
20
14
20
20
16
19
18
8
11
7
24
13
20
19
16
25
22
8
8
4
15
11
9
8
31
19
13
14
10
24
23
10
12
19
10
6
5
20
14
15
18
14
24
21
9
8
4
16
6
8
5
29
12
10
14
7
26
23
7
11
12
12
14
15
15
16
16
16
16
16
16
16
16
18
20
21
21
21
22
22
22
23
24
25
25
27
6
19
14
0
4
18
11
15
14
12
19
12
6
11
11
6
16
5
1
26
11
18
18
13
17
20
9
13
17
6
5
1
20
12
15
19
8
15
10
1
14
9
8
16
5
8
23
20
20
13
19
16
17
12
6
19
16
1
5
18
14
19
12
14
22
15
11
12
8
5
16
1
8
26
9
16
20
9
20
20
6
27
22
29
26
23
14
28
20
16
19
16
23
24
26
31
29
27
26
23
26
25
26
19
33
19
12
27
10
26
20
8
19
13
16
11
16
12
16
16
18
23
19
12
23
11
20
9
25
24
22
13
16
25
14
32
25
14
19
18
23
24
19
17
26
23
26
15
3
13
10
22
18
20
16
26
24
14
19
18
24
20
24
17
16
20
13
18
26
19
10
13
17
16
12
20
14
14
22
18
13
20
19
22
14
28
9
21
13
14
19
16
10
16
20
14
17
26
22
19
22
17
14
10
7
16
13
19
9
33
13
19
28
25
22
15
20
21
23
20
16
18
19
14
8
21
15
14
17
20
24
26
24
17
16
20
19
16
18
9
25
13
20
19
13
19
20
17
11
17
14
14
17
17
11
18
20
23
23
21
20
17
20
12
25
24
21
22
13
25
16
24
18
9
10
20
23
22
13
11
25
26
13
16
10
7
21
9
12
6
27
14
20
13
15
20
25

Graph97

Apex-Goeland
Eurol-Prestol
Eurol-Andol
Apex-Envol
Apex-Express
Envol-Lady
Akamar-Doublol
Contact-Navajo
Ceres-Goeland
Ceres-Lady
Apex-Ceres
Columbus-Tapidor
Goeland-Lady
Apex-Lady
212
16 8 varieacuteteacutes preacutesentes
16 10 varieacuteteacutes preacutesentes
756
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19961997
18
13
13
18
9
17
24
9
6
4
8
13
15
13
9
10
8
13
10
20
8
15
16
20
6
12
13
18
13
19
14
19
19
15
24
24
20
17
22
17
18
22
17
20
17
17
12
18
18
21
11
20
7
24
13
13
15
18
21
20
18
22
18
18
19
9
3
12
18
15
18
13
9
15
18
9
19
18
18
13
19
13
19
15
19
15
4
12
15
12
16
16
5
14
2
8
8
3
17
6
25
10
16
23
10
15
15
8
18
14
18
19
19
17
15
17
13
22
13
21
16
18
19
21
10
9
11
15
17
16
15
26
14
10
19
9
19
21
15
19
25
26
15
13
9
9
22
23
16
11
16
15
19
17
23
14
21
17
18
11
17
15
17
19
20
4
17
25
10
21
11
15
26
12
10
12
10
11
5
16
5
24
18
17
24
20
18
15
21
24
15
18
12
15
26
10
18
20
22
23
21
13
15
20
13
10
16
12
15
20
13
18
11
17
12
18
9
24
22
15
17
15
21
18
14
12
15
23
22
15
22
17
9
20
20
16
11
18
12
24
5
19
16
6
24
18
12
13
13
11
20
14
7
8
13
14
18
6
11
10
8
8
14
3
16
15
21
12
13
20
4
20
18
16
22
9
15
22
12
7
10
19
18
20
9
10
16
17
15
14
10
18
16
23
10
15
22
8
17
19
16
13
9
26
23
15
8
10
17
23
19
12
22
19
9
14
13
9
23
12
25
15
13
18
13
22
9
5
21
22
12
21
23
13
19
17
11
10
12
11
8
10
1
20
12
16
24
21
19
19
21
15
17
3
14
16
23
10
13
22
14
18
23
11
16
13
12
14
10
8
17
11
2
19
20
20
17
26
13
18
12
2
18
16
12
15
15
18
20
19
10
16
15
15
8
18
11
29
9
18
22
8
18
19
6
9
22
18
8
19
11
10
20
22
6
9
12
12
13
15
13
11
11
7
22
7
16
22
18
19
15
22
10
17
15
8
21
16
11
13
17
11
10
22
11
12
15
13
8
17
11
23
11
13
19
20
16
19
22
8
20
18
3
10
15
5
10
9
10
16
19
8
14
8
11
8
15
9
23
11
15
21
19
12
12
10
13
17
13
17
9
19
16
16
20
8
17
9
10
10
18
11
17
15
8
10
8
11
10
24
19
15
23
10
17
9
6
11
17
5
12
20
8
15
14
1
8
11
7
11
9
8
17
13
17
20
21
15
10
16
20
12
15
25
15
23
24
15
16
14
14
13
20
17
29
22
23
23
10
17
15
17
14
23
19
13
30
8
18
18
10
17
14
18
20
11
3
10
9
12
11
9
7
11
11
8
13
15
6
10
15
15
20
19
15
18
9
16
16
21
17
13
18
16
18
23
16
2
18
16
13
15
11
17
17
6
13
20
18
20
25
16
21
19
23
15
17
24
18
12
15
16
12
24
19
22
22
19
21
10
20
14
10
13
26
9
24
21
20
11
18
10
26
18
20
11
24
21
23
25
21
20
8
18
20
19
24
21
23
15
20
26
26
15
14
6
20
18
15
14
11
18
17
5
12
10
15
19
20
18
19
16
12
19
15
19
15
18
9
26
16
14
12
7
13
15
10
17
15
12
19
13
15
13
19
17
19
15
19
12
15
10
13
20
20
24
15
16
21
13
24
19
8
19
15
21
18
16
20
22
18
21
26
6
22
22
10
23
16
30
19
25
21
14
14
21

Graph98

Type 3
Type 1
Type 4
Type 2
Express - Lady
Duetol - Tapidor
Eurol - Zorro
Ceres - Cheyenne
Express - Zorro
Zorro - Apex
Goeland - Lady
Apex - Goeland
Apex - Lady
Eurol - Idol
Bristol - Idol
Andol - Idol
Bristol - Eurol
Apex - Express
Bristol - Prestol
Andol - Eurol
Express - Goeland
Rogers Distances (Molecular marker distances)
GAIumlA Distances(Traditional characteristic distances)
GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998
24
10
12
28
7
12
16
24
14
19
19
18
18
17
11
20
12
19
17
23
6
28
27
17
17
14
17
24
19
15
15
30
11
13
29
30
20
18
20
15
12
24
25
18
17
15
20
29
22
19
13
20
14
17
10
19
8
8
25
9
10
21
24
26
15
12
5
15
9
20
9
1
11
24
15
17
15
9
24
17
11
12
15
8
10
23
7
6
22
21
24
9
11
8
2
10
24
1
7
1
20
15
19
19
8
12
18
5
28
15
8
10
32
14
10
15
32
27
11
9
2
8
16
29
10
1
7
22
29
5
7
10
12
25
8
7
30
25
23
32
25
28
31
17
18
21
30
27
27
20
34
23
26
25
24
8
29
37
30
17
24
25
12
11
9
7
14
25
4
21
22
18
8
18
11
12
11
19
10
14
10
20
18
24
17
10
18
10
13
16
13
10
6
10
28
4
17
32
19
6
10
7
7
16
25
9
6
6
20
21
16
19
7
14
15
9
24
29
21
22
15
31
21
17
28
19
7
20
21
21
20
30
25
21
23
26
34
22
26
24
11
23
19
14
30
24
21
32
17
22
32
28
16
23
29
24
24
23
32
22
24
23
14
14
30
29
30
16
14
18
19
20
26
24
27
18
18
19
19
16
15
21
22
27
31
30
29
26
28
16
21
24
27
27
10
8
20
19
18
15
9
11
21
8
6
7
23
15
7
9
10
16
24
11
10
8
14
25
16
17
9
3
14
9
18
20
12
11
9
30
18
10
20
29
21
7
7
11
23
23
16
11
12
18
22
10
17
11
13
21
7
18
15
5
8
2
27
11
7
21
24
22
9
7
12
16
22
9
1
7
17
22
8
11
9
11
16
4
17
12
15
2
8
27
12
7
21
24
27
10
11
12
12
27
5
10
1
18
21
13
17
15
8
22
4
11
24
9
10
16
20
11
16
20
23
31
16
23
16
12
16
7
13
9
29
12
25
22
11
21
24
15
20
25
20
24
29
34
19
25
30
32
30
24
23
22
27
16
19
22
22
33
26
35
32
8
32
18
26
12
18
9
1
10
23
10
9
25
22
29
11
16
9
5
7
19
8
0
25
14
21
25
10
14
20
8
19
17
1
7
1
26
14
6
21
24
26
10
11
1
10
13
22
8
8
20
22
12
14
12
16
20
7
17
15
11
1
7
25
10
6
23
23
28
8
12
7
1
9
22
0
8
22
20
19
19
9
11
21
6
23
20
24
20
22
24
20
20
26
14
16
14
18
17
18
29
33
25
20
22
25
22
21
25
13
16
17
6
29
15
15
29
8
18
21
34
14
21
25
22
22
21
12
26
14
22
20
25
26
30
22
23
19
19
28
22
17
19
5
29
24
16
22
30
24
16
10
8
13
25
35
21
12
19
22
26
7
23
14
27
13
27
19
15
19
7
37
17
19
26
29
27
17
17
11
17
22
32
25
14
19
21
30
7
20
16
23
12
17
13
9
8
10
30
10
7
24
30
27
9
11
9
15
11
8
10
12
9
25
22
23
20
22
13
13
17
20
24
12
12
17
18
14
11
16
10
3
13
11
8
21
32
14
16
11
13
23
14
16
22
20
9
14
14
17
18
25
24
10
15
23
14
8
14
21
16
22
24
18
20
20
21
16
19
27
23
13
20
17
17
17
11
5
8
25
13
9
19
18
20
9
7
4
4
15
26
8
7
6
17
19
13
12
13
9
17

Graph99

169
677
61 20 varieacuteteacutes preacutesentes
93 19 varieacuteteacutes preacutesentes
Goeland-Lady
Goeland-Apex
Envol-Idol
Apex-Lady
Ceres-Falcon
Bristol-Eurol
Apex-Envol
Andol-Eurol
Eurol-Idol
Bristol-Prestol
Doublol-Duetol
Capitol-Vivol
Eurol-Samourai
Bristol-Samourai
Akamar-Duetol
Contact-Envol
Duetol-Navajo
Apex-Contact
Prestol-Samouraiet Goeland-Idol
Andol-Zorro
Falcon-Navajo
Andol-Falcon
Falcon-Goeland
Lady-Navajo
Duetol-Lady
Express-Zorro
Contact-Goeland
Duetol-Lady
Distances de ROGERS
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19981999
1
1
1
1
0
0
11
11
1
1
2
2
13
13
5
5
8
8
8
8
6
6
5
5
3
7
3
7
8
10
8
10
8
8
5
5
15
15
15
15
6
6
6
6
5
5
13
13
1
1
14
14
18
18
3
3
2
2
3
3
15
15
12
12
13
13
8
8
12
18
12
18
17
17
20
20
12
12
24
2
24
2
5
20
2
20
5
11
2
11
13
13
1
1
20
20
3
16
3
16
15
15
2
8
8
2
8
8
11
11
12
12
15
15
2
4
4
2
8
13
8
13
17
19
19
17
13
13
11
14
11
14
13
13
12
17
8
12
7
20
20
7
17
8
8
8
11
11
9
9
9
9
12
18
12
18
8
8
15
15
15
15
10
10
5
5
8
8
20
6
6
20
13
13
2
15
15
2
6
6
10
10
16
16
14
6
14
6
15
21
15
21
7
7
21
21
20
7
7
20
15
15
7
7
14
6
6
9
2
2
14
9
10
10
16
16
15
15
13
6
13
6
11
11
19
8
10
10
22
8
22
19
5
5
4
5
4
5
5
7
5
15
5
12
15
12
7
5
0
12
6
0
6
12
22
11
14
14
13
22
11
13
10
6
20
6
20
10
4
15
1
4
1
8
11
15
8
11
18
18
13
15
13
15
13
13
5
17
5
9
3
3
15
12
15
5
12
5
17
9
12
12
14
14
8
10
13
10
13
8
23
6
12
9
6
9
12
23
14
12
10
12
14
10
14
14
16
12
13
12
10
13
10
16
12
15
13
12
9
10
9
5
15
13
2
5
2
13
10
13
4
9
17
9
4
17
10
12
9
10
12
9
16
8
8
12
8
16
12
8
13
13
6
3
6
3
11
12
3
20
12
20
14
11
3
14
18
7
7
18
19
19
13
19
19
11
13
11
3
18
2
20
28
3
20
2
28
12
18
12
5
13
8
12
13
5
8
8
12
8
13
9
13
12
9
16
12
18
18
16
10
10
16
15
16
15
14
11
14
11
10
16
8
16
10
8
10
2
8
12
2
6
11
23
10
6
19
8
11
23
12
19
6
6
10
7
8
1
9
21
9
21
7
1
10
8
18
14
18
14
17
17
8
8
9
5
14
3
9
21
5
16
13
14
16
13
21
3
10
5
5
10
13
13
4
15
4
15
5
8
8
8
5
8
5
5
17
15
17
15
10
8
7
8
10
9
20
20
7
9
15
12
10
15
18
12
18
10
15
23
15
23
12
12
19
19
14
14
14
11
11
8
14
8
17
6
17
6
13
13
10
2
2
5
18
10
5
18
10
7
10
13
15
13
15
15
13
15
13
7
2
2
8
8
14
7
10
14
7
10
11
14
11
18
14
18
13
13
16
16
15
16
16
15
9
2
11
2
9
12
12
11
12
15
12
15
8
11
12
14
14
8
12
11
11
24
11
24
13
13
13
15
16
16
22
13
15
16
22
13
15
15
16
14
13
12
14
12
10
10
15
10
6
10
15
6
9
9
13
13
10
11
11
10
14
14
10
10
17
8
7
8
8
17
8
7
8
8
12
2
2
12
17
17
12
12
18
18
18
18
5
8
5
16
16
8
14
14
14
8
22
22
13
13
14
14
9
14
9
8
13
13
11
13
11
13
9
9
12
12
9
9
13
13
10
10
13
13
6
6
11
11
17
17
9
9
15
15
9
9
6
6
12
12
24
24
8
8
12
19
12
19
9
9
12
12

Model 1 Characteristic-specific molecular markers

Example 1 gene specific marker for herbicide tolerance introduced by genetic modification On the basis that [hellip] - there is verification of the reliability of the link between the marker and the characteristic - different markers for the same characteristic are different methods for examining the same characteristic [hellip]

Fusarium race 0 ex 1 Example Characteristic-specific molecular marker with incomplete information on state of expression

Model 1 Characteristic-specific molecular markers

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

ex Maize parent lines

Model 2 Combining phenotypic and molecular distances in the management of variety collections

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

2

02

Model 2 Combining phenotypic and molecular distances in the management of variety collections (continued)

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

13

New Model 3 Genetic selection of similar varieties for the first growing cycle

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
0038
0038
0042
0042
0045
0045
0063
0063
0067
0067
0068
0068
0075
0075
0106
0106
011
011
0114
0114
0115
0115
0116
0116
0123
0123
0123
0123
0131
0131
0131
0131
0132
0132
0145
0145
0149
0149
0149
0149
017
017
0176
0176
018
018
0185
0185
0186
0186
0194
0194
0198
0198
0199
0199
02
02
0201
0201
0202
0202
0203
0203
0204
0204
0207
0207
021
021
021
021
0213
0213
0214
0214
0217
0217
0217
0217
0217
0217
0219
0219
0219
0219
0219
0219
0219
0219
022
022
0224
0224
0225
0225
0225
0225
0225
0225
0226
0226
0227
0227
0227
0227
0227
0227
0228
0228
0228
0228
0228
0228
023
023
023
023
023
023
023
023
0231
0231
0231
0231
0232
0232
0232
0232
0232
0232
0233
0233
0235
0235
0235
0235
0235
0235
0235
0235
0235
0235
0236
0236
0236
0236
0238
0238
0238
0238
0239
0239
0239
0239
0241
0241
0242
0242
0244
0244
0245
0245
0246
0246
0247
0247
0249
0249
0249
0249
025
025
0251
0251
0251
0251
0252
0252
0252
0252
0252
0252
0253
0253
0253
0253
0254
0254
0254
0254
0255
0255
0259
0259
0259
0259
0259
0259
026
026
026
026
0262
0262
0262
0262
0262
0262
0262
0262
0264
0264
0265
0265
0266
0266
0267
0267
0267
0267
0268
0268
0269
0269
0269
0269
0269
0269
0269
0269
027
027
027
027
027
027
0272
0272
0272
0272
0272
0272
0272
0272
0272
0272
0273
0273
0273
0273
0273
0273
0275
0275
0275
0275
0275
0275
0275
0275
0276
0276
0276
0276
0276
0276
0278
0278
0278
0278
0278
0278
0278
0278
0278
0278
0279
0279
0279
0279
0279
0279
028
028
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0282
0282
0282
0282
0283
0283
0283
0283
0283
0283
0284
0284
0284
0284
0284
0284
0284
0284
0285
0285
0285
0285
0285
0285
0285
0285
0286
0286
0286
0286
0286
0286
0286
0286
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0288
0288
0288
0288
0288
0288
0289
0289
0289
0289
0289
0289
029
029
029
029
029
029
029
029
0291
0291
0292
0292
0292
0292
0293
0293
0293
0293
0293
0293
0293
0293
0293
0293
0294
0294
0294
0294
0294
0294
0295
0295
0295
0295
0295
0295
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0298
0298
0298
0298
0298
0298
0298
0298
0298
0298
0299
0299
0299
0299
0299
0299
0299
0299
0299
0299
03
03
0302
0302
0302
0302
0303
0303
0303
0303
0304
0304
0304
0304
0304
0304
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0307
0307
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
031
031
031
031
031
031
0311
0311
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0313
0313
0313
0313
0314
0314
0315
0315
0315
0315
0316
0316
0316
0316
0316
0316
0318
0318
0318
0318
0318
0318
0319
0319
0319
0319
0319
0319
0319
0319
0319
0319
032
032
032
032
032
032
032
032
0321
0321
0321
0321
0322
0322
0322
0322
0323
0323
0324
0324
0324
0324
0324
0324
0325
0325
0325
0325
0326
0326
0327
0327
0327
0327
0327
0327
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0282
0284
0272
0342
0405
0312
0273
0149
0336
027
0278
0345
0068
0299
0285
0312
025
0284
0294
0309
0327
0231
0262
0287
0296
0269
0358
0342
029
0265
0304
0335
0287
0245
0239
034
0213
0312
0351
0225
0235
0217
0253
0259
0293
0281
0309
0293
0231
0198
0296
0219
0296
0299
0342
0306
0207
032
0345
021
027
028
0272
0038
0227
0358
0281
0236
0202
0246
0275
0288
0217
0249
023
0262
0198
0201
0045
0319
0313
0244
0236
0204
0285
0321
0304
0289
0289
0123
0219
0114
0348
0306
0346
0294
0296
029
0306
0042
0106
0281
0287
0296
0201
0225
0315
038
0063
0194
0367
0225
0364
0352
0292
0298
0287
0067
0247
0351
0309
0269
0232
0281
0299
0315
023
0276
0253
0296
0219
0045
0225
0299
0327
0251
0232
0228
031
0335
0324
0295
0252
0333
0327
0306
0275
0275
0302
0254
0302
0185
0231
0288
0322
0398
0269
0296
0319
0315
0299
0361
0339
0294
0318
0354
0316
0321
0292
0342
0404
0298
0383
0351
0327
033
0331
0306
0312
0309
0377
0412
0389
0358
0299
0313
038
0327
0361
0395
0368
0272
0389
0332
0336
0312
0312
0132
0242
0116
0364
0357
0376
034
0342
0323
032
0075
0115
0282
0342
0342
0244
0063
0251
0339
0395
0199
0361
0232
0338
037
0318
0282
0235
0233
02
0359
0281
0342
0285
029
0287
0306
021
0219
0284
029
0306
0236
0194
0232
0294
0368
0199
0342
0235
0338
0303
023
0267
038
0203
0346
0364
0278
0176
0214
0281
0312
029
0345
0329
0272
0265
0207
0204
0367
0228
0318
0272
0361
0342
0358
0345
0287
0283
0325
0266
0293
0259
0361
031
0361
0356
0339
0326
0339
0239
0276
0342
0304
032
0285
0225
031
0354
0389
0232
0235
0358
0342
0373
0296
0287
0386
0329
0329
018
0335
0361
0286
0339
0307
032
0334
0372
0405
0335
0345
0321
0364
0335
0316
0332
0338
0338
0345
0342
031
0295
0252
0352
0298
0336
0342
0269
0235
0279
0235
0284
0287
0329
0319
0312
0287
021
0304
0352
0324
0321
0336
037
0303
0287
0373
031
Page 9: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

Graph96

Express-Falcon
Falcon-Lady
Bristol-Falcon
Doublol-Cheyenne
Akamar-Apex
Apex-Goeland
Envol-Idol
Goeland-Lady
Apex-Lady
Bristol-Idol
Eurol-Prestol
Andol-Envol
Bristol-Envol
Idol-Goeland
Apex-Bristol
Apex-Idol
Bristol--Lady
Bristol-Goeland
34 9 varieacuteteacutes preacutesentes
193
759
14 8 varieacuteteacutes preacutesentes
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19951996
23
14
3
14
11
10
20
16
9
24
11
18
21
19
12
22
6
13
6
27
10
32
24
14
20
19
16
23
14
19
19
22
21
14
22
19
13
11
16
21
18
19
11
19
17
19
22
26
25
17
19
21
13
24
14
14
14
9
20
9
11
22
14
21
16
5
10
8
10
16
14
6
16
29
20
14
16
16
23
19
18
3
19
14
4
18
11
16
14
12
19
12
8
13
11
6
16
0
5
1
26
8
19
20
10
20
20
9
14
19
9
4
20
13
15
19
11
16
11
2
11
7
5
16
4
1
5
23
19
18
13
16
16
17
10
11
22
20
18
20
18
13
10
13
23
17
22
24
24
20
25
18
20
18
14
13
23
18
20
18
11
20
10
21
9
11
13
18
10
19
19
17
8
16
14
13
14
20
11
12
14
28
16
24
26
14
19
17
23
20
14
11
16
15
13
10
20
14
3
10
10
17
20
15
14
15
15
19
20
11
19
19
17
14
14
22
16
22
22
14
19
10
19
20
10
20
16
20
20
19
18
25
14
19
12
16
16
17
10
26
8
14
13
11
11
16
12
11
17
8
10
16
19
13
13
18
22
21
12
12
10
15
23
16
26
16
22
14
11
26
18
16
5
8
2
22
16
10
20
11
14
13
13
8
9
15
6
1
11
24
18
15
12
17
17
18
13
9
19
14
12
11
13
19
14
10
18
19
11
24
16
14
21
12
8
14
19
12
26
13
22
21
17
11
24
13
21
19
16
23
17
3
20
18
13
14
21
25
24
12
19
15
22
16
16
23
17
19
15
17
25
21
21
10
13
11
24
14
17
20
24
21
18
13
8
8
18
11
14
12
26
23
3
20
14
20
20
16
19
18
8
11
7
24
13
20
19
16
25
22
8
8
4
15
11
9
8
31
19
13
14
10
24
23
10
12
19
10
6
5
20
14
15
18
14
24
21
9
8
4
16
6
8
5
29
12
10
14
7
26
23
7
11
12
12
14
15
15
16
16
16
16
16
16
16
16
18
20
21
21
21
22
22
22
23
24
25
25
27
6
19
14
0
4
18
11
15
14
12
19
12
6
11
11
6
16
5
1
26
11
18
18
13
17
20
9
13
17
6
5
1
20
12
15
19
8
15
10
1
14
9
8
16
5
8
23
20
20
13
19
16
17
12
6
19
16
1
5
18
14
19
12
14
22
15
11
12
8
5
16
1
8
26
9
16
20
9
20
20
6
27
22
29
26
23
14
28
20
16
19
16
23
24
26
31
29
27
26
23
26
25
26
19
33
19
12
27
10
26
20
8
19
13
16
11
16
12
16
16
18
23
19
12
23
11
20
9
25
24
22
13
16
25
14
32
25
14
19
18
23
24
19
17
26
23
26
15
3
13
10
22
18
20
16
26
24
14
19
18
24
20
24
17
16
20
13
18
26
19
10
13
17
16
12
20
14
14
22
18
13
20
19
22
14
28
9
21
13
14
19
16
10
16
20
14
17
26
22
19
22
17
14
10
7
16
13
19
9
33
13
19
28
25
22
15
20
21
23
20
16
18
19
14
8
21
15
14
17
20
24
26
24
17
16
20
19
16
18
9
25
13
20
19
13
19
20
17
11
17
14
14
17
17
11
18
20
23
23
21
20
17
20
12
25
24
21
22
13
25
16
24
18
9
10
20
23
22
13
11
25
26
13
16
10
7
21
9
12
6
27
14
20
13
15
20
25

Graph97

Apex-Goeland
Eurol-Prestol
Eurol-Andol
Apex-Envol
Apex-Express
Envol-Lady
Akamar-Doublol
Contact-Navajo
Ceres-Goeland
Ceres-Lady
Apex-Ceres
Columbus-Tapidor
Goeland-Lady
Apex-Lady
212
16 8 varieacuteteacutes preacutesentes
16 10 varieacuteteacutes preacutesentes
756
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19961997
18
13
13
18
9
17
24
9
6
4
8
13
15
13
9
10
8
13
10
20
8
15
16
20
6
12
13
18
13
19
14
19
19
15
24
24
20
17
22
17
18
22
17
20
17
17
12
18
18
21
11
20
7
24
13
13
15
18
21
20
18
22
18
18
19
9
3
12
18
15
18
13
9
15
18
9
19
18
18
13
19
13
19
15
19
15
4
12
15
12
16
16
5
14
2
8
8
3
17
6
25
10
16
23
10
15
15
8
18
14
18
19
19
17
15
17
13
22
13
21
16
18
19
21
10
9
11
15
17
16
15
26
14
10
19
9
19
21
15
19
25
26
15
13
9
9
22
23
16
11
16
15
19
17
23
14
21
17
18
11
17
15
17
19
20
4
17
25
10
21
11
15
26
12
10
12
10
11
5
16
5
24
18
17
24
20
18
15
21
24
15
18
12
15
26
10
18
20
22
23
21
13
15
20
13
10
16
12
15
20
13
18
11
17
12
18
9
24
22
15
17
15
21
18
14
12
15
23
22
15
22
17
9
20
20
16
11
18
12
24
5
19
16
6
24
18
12
13
13
11
20
14
7
8
13
14
18
6
11
10
8
8
14
3
16
15
21
12
13
20
4
20
18
16
22
9
15
22
12
7
10
19
18
20
9
10
16
17
15
14
10
18
16
23
10
15
22
8
17
19
16
13
9
26
23
15
8
10
17
23
19
12
22
19
9
14
13
9
23
12
25
15
13
18
13
22
9
5
21
22
12
21
23
13
19
17
11
10
12
11
8
10
1
20
12
16
24
21
19
19
21
15
17
3
14
16
23
10
13
22
14
18
23
11
16
13
12
14
10
8
17
11
2
19
20
20
17
26
13
18
12
2
18
16
12
15
15
18
20
19
10
16
15
15
8
18
11
29
9
18
22
8
18
19
6
9
22
18
8
19
11
10
20
22
6
9
12
12
13
15
13
11
11
7
22
7
16
22
18
19
15
22
10
17
15
8
21
16
11
13
17
11
10
22
11
12
15
13
8
17
11
23
11
13
19
20
16
19
22
8
20
18
3
10
15
5
10
9
10
16
19
8
14
8
11
8
15
9
23
11
15
21
19
12
12
10
13
17
13
17
9
19
16
16
20
8
17
9
10
10
18
11
17
15
8
10
8
11
10
24
19
15
23
10
17
9
6
11
17
5
12
20
8
15
14
1
8
11
7
11
9
8
17
13
17
20
21
15
10
16
20
12
15
25
15
23
24
15
16
14
14
13
20
17
29
22
23
23
10
17
15
17
14
23
19
13
30
8
18
18
10
17
14
18
20
11
3
10
9
12
11
9
7
11
11
8
13
15
6
10
15
15
20
19
15
18
9
16
16
21
17
13
18
16
18
23
16
2
18
16
13
15
11
17
17
6
13
20
18
20
25
16
21
19
23
15
17
24
18
12
15
16
12
24
19
22
22
19
21
10
20
14
10
13
26
9
24
21
20
11
18
10
26
18
20
11
24
21
23
25
21
20
8
18
20
19
24
21
23
15
20
26
26
15
14
6
20
18
15
14
11
18
17
5
12
10
15
19
20
18
19
16
12
19
15
19
15
18
9
26
16
14
12
7
13
15
10
17
15
12
19
13
15
13
19
17
19
15
19
12
15
10
13
20
20
24
15
16
21
13
24
19
8
19
15
21
18
16
20
22
18
21
26
6
22
22
10
23
16
30
19
25
21
14
14
21

Graph98

Type 3
Type 1
Type 4
Type 2
Express - Lady
Duetol - Tapidor
Eurol - Zorro
Ceres - Cheyenne
Express - Zorro
Zorro - Apex
Goeland - Lady
Apex - Goeland
Apex - Lady
Eurol - Idol
Bristol - Idol
Andol - Idol
Bristol - Eurol
Apex - Express
Bristol - Prestol
Andol - Eurol
Express - Goeland
Rogers Distances (Molecular marker distances)
GAIumlA Distances(Traditional characteristic distances)
GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998
24
10
12
28
7
12
16
24
14
19
19
18
18
17
11
20
12
19
17
23
6
28
27
17
17
14
17
24
19
15
15
30
11
13
29
30
20
18
20
15
12
24
25
18
17
15
20
29
22
19
13
20
14
17
10
19
8
8
25
9
10
21
24
26
15
12
5
15
9
20
9
1
11
24
15
17
15
9
24
17
11
12
15
8
10
23
7
6
22
21
24
9
11
8
2
10
24
1
7
1
20
15
19
19
8
12
18
5
28
15
8
10
32
14
10
15
32
27
11
9
2
8
16
29
10
1
7
22
29
5
7
10
12
25
8
7
30
25
23
32
25
28
31
17
18
21
30
27
27
20
34
23
26
25
24
8
29
37
30
17
24
25
12
11
9
7
14
25
4
21
22
18
8
18
11
12
11
19
10
14
10
20
18
24
17
10
18
10
13
16
13
10
6
10
28
4
17
32
19
6
10
7
7
16
25
9
6
6
20
21
16
19
7
14
15
9
24
29
21
22
15
31
21
17
28
19
7
20
21
21
20
30
25
21
23
26
34
22
26
24
11
23
19
14
30
24
21
32
17
22
32
28
16
23
29
24
24
23
32
22
24
23
14
14
30
29
30
16
14
18
19
20
26
24
27
18
18
19
19
16
15
21
22
27
31
30
29
26
28
16
21
24
27
27
10
8
20
19
18
15
9
11
21
8
6
7
23
15
7
9
10
16
24
11
10
8
14
25
16
17
9
3
14
9
18
20
12
11
9
30
18
10
20
29
21
7
7
11
23
23
16
11
12
18
22
10
17
11
13
21
7
18
15
5
8
2
27
11
7
21
24
22
9
7
12
16
22
9
1
7
17
22
8
11
9
11
16
4
17
12
15
2
8
27
12
7
21
24
27
10
11
12
12
27
5
10
1
18
21
13
17
15
8
22
4
11
24
9
10
16
20
11
16
20
23
31
16
23
16
12
16
7
13
9
29
12
25
22
11
21
24
15
20
25
20
24
29
34
19
25
30
32
30
24
23
22
27
16
19
22
22
33
26
35
32
8
32
18
26
12
18
9
1
10
23
10
9
25
22
29
11
16
9
5
7
19
8
0
25
14
21
25
10
14
20
8
19
17
1
7
1
26
14
6
21
24
26
10
11
1
10
13
22
8
8
20
22
12
14
12
16
20
7
17
15
11
1
7
25
10
6
23
23
28
8
12
7
1
9
22
0
8
22
20
19
19
9
11
21
6
23
20
24
20
22
24
20
20
26
14
16
14
18
17
18
29
33
25
20
22
25
22
21
25
13
16
17
6
29
15
15
29
8
18
21
34
14
21
25
22
22
21
12
26
14
22
20
25
26
30
22
23
19
19
28
22
17
19
5
29
24
16
22
30
24
16
10
8
13
25
35
21
12
19
22
26
7
23
14
27
13
27
19
15
19
7
37
17
19
26
29
27
17
17
11
17
22
32
25
14
19
21
30
7
20
16
23
12
17
13
9
8
10
30
10
7
24
30
27
9
11
9
15
11
8
10
12
9
25
22
23
20
22
13
13
17
20
24
12
12
17
18
14
11
16
10
3
13
11
8
21
32
14
16
11
13
23
14
16
22
20
9
14
14
17
18
25
24
10
15
23
14
8
14
21
16
22
24
18
20
20
21
16
19
27
23
13
20
17
17
17
11
5
8
25
13
9
19
18
20
9
7
4
4
15
26
8
7
6
17
19
13
12
13
9
17

Graph99

169
677
61 20 varieacuteteacutes preacutesentes
93 19 varieacuteteacutes preacutesentes
Goeland-Lady
Goeland-Apex
Envol-Idol
Apex-Lady
Ceres-Falcon
Bristol-Eurol
Apex-Envol
Andol-Eurol
Eurol-Idol
Bristol-Prestol
Doublol-Duetol
Capitol-Vivol
Eurol-Samourai
Bristol-Samourai
Akamar-Duetol
Contact-Envol
Duetol-Navajo
Apex-Contact
Prestol-Samouraiet Goeland-Idol
Andol-Zorro
Falcon-Navajo
Andol-Falcon
Falcon-Goeland
Lady-Navajo
Duetol-Lady
Express-Zorro
Contact-Goeland
Duetol-Lady
Distances de ROGERS
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19981999
1
1
1
1
0
0
11
11
1
1
2
2
13
13
5
5
8
8
8
8
6
6
5
5
3
7
3
7
8
10
8
10
8
8
5
5
15
15
15
15
6
6
6
6
5
5
13
13
1
1
14
14
18
18
3
3
2
2
3
3
15
15
12
12
13
13
8
8
12
18
12
18
17
17
20
20
12
12
24
2
24
2
5
20
2
20
5
11
2
11
13
13
1
1
20
20
3
16
3
16
15
15
2
8
8
2
8
8
11
11
12
12
15
15
2
4
4
2
8
13
8
13
17
19
19
17
13
13
11
14
11
14
13
13
12
17
8
12
7
20
20
7
17
8
8
8
11
11
9
9
9
9
12
18
12
18
8
8
15
15
15
15
10
10
5
5
8
8
20
6
6
20
13
13
2
15
15
2
6
6
10
10
16
16
14
6
14
6
15
21
15
21
7
7
21
21
20
7
7
20
15
15
7
7
14
6
6
9
2
2
14
9
10
10
16
16
15
15
13
6
13
6
11
11
19
8
10
10
22
8
22
19
5
5
4
5
4
5
5
7
5
15
5
12
15
12
7
5
0
12
6
0
6
12
22
11
14
14
13
22
11
13
10
6
20
6
20
10
4
15
1
4
1
8
11
15
8
11
18
18
13
15
13
15
13
13
5
17
5
9
3
3
15
12
15
5
12
5
17
9
12
12
14
14
8
10
13
10
13
8
23
6
12
9
6
9
12
23
14
12
10
12
14
10
14
14
16
12
13
12
10
13
10
16
12
15
13
12
9
10
9
5
15
13
2
5
2
13
10
13
4
9
17
9
4
17
10
12
9
10
12
9
16
8
8
12
8
16
12
8
13
13
6
3
6
3
11
12
3
20
12
20
14
11
3
14
18
7
7
18
19
19
13
19
19
11
13
11
3
18
2
20
28
3
20
2
28
12
18
12
5
13
8
12
13
5
8
8
12
8
13
9
13
12
9
16
12
18
18
16
10
10
16
15
16
15
14
11
14
11
10
16
8
16
10
8
10
2
8
12
2
6
11
23
10
6
19
8
11
23
12
19
6
6
10
7
8
1
9
21
9
21
7
1
10
8
18
14
18
14
17
17
8
8
9
5
14
3
9
21
5
16
13
14
16
13
21
3
10
5
5
10
13
13
4
15
4
15
5
8
8
8
5
8
5
5
17
15
17
15
10
8
7
8
10
9
20
20
7
9
15
12
10
15
18
12
18
10
15
23
15
23
12
12
19
19
14
14
14
11
11
8
14
8
17
6
17
6
13
13
10
2
2
5
18
10
5
18
10
7
10
13
15
13
15
15
13
15
13
7
2
2
8
8
14
7
10
14
7
10
11
14
11
18
14
18
13
13
16
16
15
16
16
15
9
2
11
2
9
12
12
11
12
15
12
15
8
11
12
14
14
8
12
11
11
24
11
24
13
13
13
15
16
16
22
13
15
16
22
13
15
15
16
14
13
12
14
12
10
10
15
10
6
10
15
6
9
9
13
13
10
11
11
10
14
14
10
10
17
8
7
8
8
17
8
7
8
8
12
2
2
12
17
17
12
12
18
18
18
18
5
8
5
16
16
8
14
14
14
8
22
22
13
13
14
14
9
14
9
8
13
13
11
13
11
13
9
9
12
12
9
9
13
13
10
10
13
13
6
6
11
11
17
17
9
9
15
15
9
9
6
6
12
12
24
24
8
8
12
19
12
19
9
9
12
12

Model 1 Characteristic-specific molecular markers

Example 1 gene specific marker for herbicide tolerance introduced by genetic modification On the basis that [hellip] - there is verification of the reliability of the link between the marker and the characteristic - different markers for the same characteristic are different methods for examining the same characteristic [hellip]

Fusarium race 0 ex 1 Example Characteristic-specific molecular marker with incomplete information on state of expression

Model 1 Characteristic-specific molecular markers

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

ex Maize parent lines

Model 2 Combining phenotypic and molecular distances in the management of variety collections

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

2

02

Model 2 Combining phenotypic and molecular distances in the management of variety collections (continued)

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

13

New Model 3 Genetic selection of similar varieties for the first growing cycle

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
0038
0038
0042
0042
0045
0045
0063
0063
0067
0067
0068
0068
0075
0075
0106
0106
011
011
0114
0114
0115
0115
0116
0116
0123
0123
0123
0123
0131
0131
0131
0131
0132
0132
0145
0145
0149
0149
0149
0149
017
017
0176
0176
018
018
0185
0185
0186
0186
0194
0194
0198
0198
0199
0199
02
02
0201
0201
0202
0202
0203
0203
0204
0204
0207
0207
021
021
021
021
0213
0213
0214
0214
0217
0217
0217
0217
0217
0217
0219
0219
0219
0219
0219
0219
0219
0219
022
022
0224
0224
0225
0225
0225
0225
0225
0225
0226
0226
0227
0227
0227
0227
0227
0227
0228
0228
0228
0228
0228
0228
023
023
023
023
023
023
023
023
0231
0231
0231
0231
0232
0232
0232
0232
0232
0232
0233
0233
0235
0235
0235
0235
0235
0235
0235
0235
0235
0235
0236
0236
0236
0236
0238
0238
0238
0238
0239
0239
0239
0239
0241
0241
0242
0242
0244
0244
0245
0245
0246
0246
0247
0247
0249
0249
0249
0249
025
025
0251
0251
0251
0251
0252
0252
0252
0252
0252
0252
0253
0253
0253
0253
0254
0254
0254
0254
0255
0255
0259
0259
0259
0259
0259
0259
026
026
026
026
0262
0262
0262
0262
0262
0262
0262
0262
0264
0264
0265
0265
0266
0266
0267
0267
0267
0267
0268
0268
0269
0269
0269
0269
0269
0269
0269
0269
027
027
027
027
027
027
0272
0272
0272
0272
0272
0272
0272
0272
0272
0272
0273
0273
0273
0273
0273
0273
0275
0275
0275
0275
0275
0275
0275
0275
0276
0276
0276
0276
0276
0276
0278
0278
0278
0278
0278
0278
0278
0278
0278
0278
0279
0279
0279
0279
0279
0279
028
028
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0282
0282
0282
0282
0283
0283
0283
0283
0283
0283
0284
0284
0284
0284
0284
0284
0284
0284
0285
0285
0285
0285
0285
0285
0285
0285
0286
0286
0286
0286
0286
0286
0286
0286
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0288
0288
0288
0288
0288
0288
0289
0289
0289
0289
0289
0289
029
029
029
029
029
029
029
029
0291
0291
0292
0292
0292
0292
0293
0293
0293
0293
0293
0293
0293
0293
0293
0293
0294
0294
0294
0294
0294
0294
0295
0295
0295
0295
0295
0295
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0298
0298
0298
0298
0298
0298
0298
0298
0298
0298
0299
0299
0299
0299
0299
0299
0299
0299
0299
0299
03
03
0302
0302
0302
0302
0303
0303
0303
0303
0304
0304
0304
0304
0304
0304
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0307
0307
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
031
031
031
031
031
031
0311
0311
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0313
0313
0313
0313
0314
0314
0315
0315
0315
0315
0316
0316
0316
0316
0316
0316
0318
0318
0318
0318
0318
0318
0319
0319
0319
0319
0319
0319
0319
0319
0319
0319
032
032
032
032
032
032
032
032
0321
0321
0321
0321
0322
0322
0322
0322
0323
0323
0324
0324
0324
0324
0324
0324
0325
0325
0325
0325
0326
0326
0327
0327
0327
0327
0327
0327
0327
0327
0329
0329
0329
0329
0329
0329
0329
0329
0329
0329
0329
0329
033
033
0331
0331
0332
0332
0332
0332
0332
0332
0333
0333
0333
0333
0333
0333
0334
0334
0335
0335
0335
0335
0335
0335
0336
0336
0336
0336
0336
0336
0336
0336
0338
0338
0338
0338
0339
0339
0339
0339
0339
0339
0339
0339
034
034
034
034
0341
0341
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0345
0345
0345
0345
0345
0345
0345
0345
0346
0346
0346
0346
0348
0348
0348
0348
0349
0349
035
035
0351
0351
0351
0351
0351
0351
0351
0351
0352
0352
0352
0352
0352
0352
0354
0354
0355
0355
0356
0356
0357
0357
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023
0276
0253
0296
0219
0045
0225
0299
0327
0251
0232
0228
031
0335
0324
0295
0252
0333
0327
0306
0275
0275
0302
0254
0302
0185
0231
0288
0322
0398
0269
0296
0319
0315
0299
0361
0339
0294
0318
0354
0316
0321
0292
0342
0404
0298
0383
0351
0327
033
0331
0306
0312
0309
0377
0412
0389
0358
0299
0313
038
0327
0361
0395
0368
0272
0389
0332
0336
0312
0312
0132
0242
0116
0364
0357
0376
034
0342
0323
032
0075
0115
0282
0342
0342
0244
0063
0251
0339
0395
0199
0361
0232
0338
037
0318
0282
0235
0233
02
0359
0281
0342
0285
029
0287
0306
021
0219
0284
029
0306
0236
0194
0232
0294
0368
0199
0342
0235
0338
0303
023
0267
038
0203
0346
0364
0278
0176
0214
0281
0312
029
0345
0329
0272
0265
0207
0204
0367
0228
0318
0272
0361
0342
0358
0345
0287
0283
0325
0266
0293
0259
0361
031
0361
0356
0339
0326
0339
0239
0276
0342
0304
032
0285
0225
031
0354
0389
0232
0235
0358
0342
0373
0296
0287
0386
0329
0329
018
0335
0361
0286
0339
0307
032
0334
0372
0405
0335
0345
0321
0364
0335
0316
0332
0338
0338
0345
0342
031
0295
0252
0352
0298
0336
0342
0269
0235
0279
0235
0284
0287
0329
0319
0312
0287
021
0304
0352
0324
0321
0336
037
0303
0287
0373
031
Page 10: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

Graph97

Apex-Goeland
Eurol-Prestol
Eurol-Andol
Apex-Envol
Apex-Express
Envol-Lady
Akamar-Doublol
Contact-Navajo
Ceres-Goeland
Ceres-Lady
Apex-Ceres
Columbus-Tapidor
Goeland-Lady
Apex-Lady
212
16 8 varieacuteteacutes preacutesentes
16 10 varieacuteteacutes preacutesentes
756
Distances de Rogers
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19961997
18
13
13
18
9
17
24
9
6
4
8
13
15
13
9
10
8
13
10
20
8
15
16
20
6
12
13
18
13
19
14
19
19
15
24
24
20
17
22
17
18
22
17
20
17
17
12
18
18
21
11
20
7
24
13
13
15
18
21
20
18
22
18
18
19
9
3
12
18
15
18
13
9
15
18
9
19
18
18
13
19
13
19
15
19
15
4
12
15
12
16
16
5
14
2
8
8
3
17
6
25
10
16
23
10
15
15
8
18
14
18
19
19
17
15
17
13
22
13
21
16
18
19
21
10
9
11
15
17
16
15
26
14
10
19
9
19
21
15
19
25
26
15
13
9
9
22
23
16
11
16
15
19
17
23
14
21
17
18
11
17
15
17
19
20
4
17
25
10
21
11
15
26
12
10
12
10
11
5
16
5
24
18
17
24
20
18
15
21
24
15
18
12
15
26
10
18
20
22
23
21
13
15
20
13
10
16
12
15
20
13
18
11
17
12
18
9
24
22
15
17
15
21
18
14
12
15
23
22
15
22
17
9
20
20
16
11
18
12
24
5
19
16
6
24
18
12
13
13
11
20
14
7
8
13
14
18
6
11
10
8
8
14
3
16
15
21
12
13
20
4
20
18
16
22
9
15
22
12
7
10
19
18
20
9
10
16
17
15
14
10
18
16
23
10
15
22
8
17
19
16
13
9
26
23
15
8
10
17
23
19
12
22
19
9
14
13
9
23
12
25
15
13
18
13
22
9
5
21
22
12
21
23
13
19
17
11
10
12
11
8
10
1
20
12
16
24
21
19
19
21
15
17
3
14
16
23
10
13
22
14
18
23
11
16
13
12
14
10
8
17
11
2
19
20
20
17
26
13
18
12
2
18
16
12
15
15
18
20
19
10
16
15
15
8
18
11
29
9
18
22
8
18
19
6
9
22
18
8
19
11
10
20
22
6
9
12
12
13
15
13
11
11
7
22
7
16
22
18
19
15
22
10
17
15
8
21
16
11
13
17
11
10
22
11
12
15
13
8
17
11
23
11
13
19
20
16
19
22
8
20
18
3
10
15
5
10
9
10
16
19
8
14
8
11
8
15
9
23
11
15
21
19
12
12
10
13
17
13
17
9
19
16
16
20
8
17
9
10
10
18
11
17
15
8
10
8
11
10
24
19
15
23
10
17
9
6
11
17
5
12
20
8
15
14
1
8
11
7
11
9
8
17
13
17
20
21
15
10
16
20
12
15
25
15
23
24
15
16
14
14
13
20
17
29
22
23
23
10
17
15
17
14
23
19
13
30
8
18
18
10
17
14
18
20
11
3
10
9
12
11
9
7
11
11
8
13
15
6
10
15
15
20
19
15
18
9
16
16
21
17
13
18
16
18
23
16
2
18
16
13
15
11
17
17
6
13
20
18
20
25
16
21
19
23
15
17
24
18
12
15
16
12
24
19
22
22
19
21
10
20
14
10
13
26
9
24
21
20
11
18
10
26
18
20
11
24
21
23
25
21
20
8
18
20
19
24
21
23
15
20
26
26
15
14
6
20
18
15
14
11
18
17
5
12
10
15
19
20
18
19
16
12
19
15
19
15
18
9
26
16
14
12
7
13
15
10
17
15
12
19
13
15
13
19
17
19
15
19
12
15
10
13
20
20
24
15
16
21
13
24
19
8
19
15
21
18
16
20
22
18
21
26
6
22
22
10
23
16
30
19
25
21
14
14
21

Graph98

Type 3
Type 1
Type 4
Type 2
Express - Lady
Duetol - Tapidor
Eurol - Zorro
Ceres - Cheyenne
Express - Zorro
Zorro - Apex
Goeland - Lady
Apex - Goeland
Apex - Lady
Eurol - Idol
Bristol - Idol
Andol - Idol
Bristol - Eurol
Apex - Express
Bristol - Prestol
Andol - Eurol
Express - Goeland
Rogers Distances (Molecular marker distances)
GAIumlA Distances(Traditional characteristic distances)
GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998
24
10
12
28
7
12
16
24
14
19
19
18
18
17
11
20
12
19
17
23
6
28
27
17
17
14
17
24
19
15
15
30
11
13
29
30
20
18
20
15
12
24
25
18
17
15
20
29
22
19
13
20
14
17
10
19
8
8
25
9
10
21
24
26
15
12
5
15
9
20
9
1
11
24
15
17
15
9
24
17
11
12
15
8
10
23
7
6
22
21
24
9
11
8
2
10
24
1
7
1
20
15
19
19
8
12
18
5
28
15
8
10
32
14
10
15
32
27
11
9
2
8
16
29
10
1
7
22
29
5
7
10
12
25
8
7
30
25
23
32
25
28
31
17
18
21
30
27
27
20
34
23
26
25
24
8
29
37
30
17
24
25
12
11
9
7
14
25
4
21
22
18
8
18
11
12
11
19
10
14
10
20
18
24
17
10
18
10
13
16
13
10
6
10
28
4
17
32
19
6
10
7
7
16
25
9
6
6
20
21
16
19
7
14
15
9
24
29
21
22
15
31
21
17
28
19
7
20
21
21
20
30
25
21
23
26
34
22
26
24
11
23
19
14
30
24
21
32
17
22
32
28
16
23
29
24
24
23
32
22
24
23
14
14
30
29
30
16
14
18
19
20
26
24
27
18
18
19
19
16
15
21
22
27
31
30
29
26
28
16
21
24
27
27
10
8
20
19
18
15
9
11
21
8
6
7
23
15
7
9
10
16
24
11
10
8
14
25
16
17
9
3
14
9
18
20
12
11
9
30
18
10
20
29
21
7
7
11
23
23
16
11
12
18
22
10
17
11
13
21
7
18
15
5
8
2
27
11
7
21
24
22
9
7
12
16
22
9
1
7
17
22
8
11
9
11
16
4
17
12
15
2
8
27
12
7
21
24
27
10
11
12
12
27
5
10
1
18
21
13
17
15
8
22
4
11
24
9
10
16
20
11
16
20
23
31
16
23
16
12
16
7
13
9
29
12
25
22
11
21
24
15
20
25
20
24
29
34
19
25
30
32
30
24
23
22
27
16
19
22
22
33
26
35
32
8
32
18
26
12
18
9
1
10
23
10
9
25
22
29
11
16
9
5
7
19
8
0
25
14
21
25
10
14
20
8
19
17
1
7
1
26
14
6
21
24
26
10
11
1
10
13
22
8
8
20
22
12
14
12
16
20
7
17
15
11
1
7
25
10
6
23
23
28
8
12
7
1
9
22
0
8
22
20
19
19
9
11
21
6
23
20
24
20
22
24
20
20
26
14
16
14
18
17
18
29
33
25
20
22
25
22
21
25
13
16
17
6
29
15
15
29
8
18
21
34
14
21
25
22
22
21
12
26
14
22
20
25
26
30
22
23
19
19
28
22
17
19
5
29
24
16
22
30
24
16
10
8
13
25
35
21
12
19
22
26
7
23
14
27
13
27
19
15
19
7
37
17
19
26
29
27
17
17
11
17
22
32
25
14
19
21
30
7
20
16
23
12
17
13
9
8
10
30
10
7
24
30
27
9
11
9
15
11
8
10
12
9
25
22
23
20
22
13
13
17
20
24
12
12
17
18
14
11
16
10
3
13
11
8
21
32
14
16
11
13
23
14
16
22
20
9
14
14
17
18
25
24
10
15
23
14
8
14
21
16
22
24
18
20
20
21
16
19
27
23
13
20
17
17
17
11
5
8
25
13
9
19
18
20
9
7
4
4
15
26
8
7
6
17
19
13
12
13
9
17

Graph99

169
677
61 20 varieacuteteacutes preacutesentes
93 19 varieacuteteacutes preacutesentes
Goeland-Lady
Goeland-Apex
Envol-Idol
Apex-Lady
Ceres-Falcon
Bristol-Eurol
Apex-Envol
Andol-Eurol
Eurol-Idol
Bristol-Prestol
Doublol-Duetol
Capitol-Vivol
Eurol-Samourai
Bristol-Samourai
Akamar-Duetol
Contact-Envol
Duetol-Navajo
Apex-Contact
Prestol-Samouraiet Goeland-Idol
Andol-Zorro
Falcon-Navajo
Andol-Falcon
Falcon-Goeland
Lady-Navajo
Duetol-Lady
Express-Zorro
Contact-Goeland
Duetol-Lady
Distances de ROGERS
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19981999
1
1
1
1
0
0
11
11
1
1
2
2
13
13
5
5
8
8
8
8
6
6
5
5
3
7
3
7
8
10
8
10
8
8
5
5
15
15
15
15
6
6
6
6
5
5
13
13
1
1
14
14
18
18
3
3
2
2
3
3
15
15
12
12
13
13
8
8
12
18
12
18
17
17
20
20
12
12
24
2
24
2
5
20
2
20
5
11
2
11
13
13
1
1
20
20
3
16
3
16
15
15
2
8
8
2
8
8
11
11
12
12
15
15
2
4
4
2
8
13
8
13
17
19
19
17
13
13
11
14
11
14
13
13
12
17
8
12
7
20
20
7
17
8
8
8
11
11
9
9
9
9
12
18
12
18
8
8
15
15
15
15
10
10
5
5
8
8
20
6
6
20
13
13
2
15
15
2
6
6
10
10
16
16
14
6
14
6
15
21
15
21
7
7
21
21
20
7
7
20
15
15
7
7
14
6
6
9
2
2
14
9
10
10
16
16
15
15
13
6
13
6
11
11
19
8
10
10
22
8
22
19
5
5
4
5
4
5
5
7
5
15
5
12
15
12
7
5
0
12
6
0
6
12
22
11
14
14
13
22
11
13
10
6
20
6
20
10
4
15
1
4
1
8
11
15
8
11
18
18
13
15
13
15
13
13
5
17
5
9
3
3
15
12
15
5
12
5
17
9
12
12
14
14
8
10
13
10
13
8
23
6
12
9
6
9
12
23
14
12
10
12
14
10
14
14
16
12
13
12
10
13
10
16
12
15
13
12
9
10
9
5
15
13
2
5
2
13
10
13
4
9
17
9
4
17
10
12
9
10
12
9
16
8
8
12
8
16
12
8
13
13
6
3
6
3
11
12
3
20
12
20
14
11
3
14
18
7
7
18
19
19
13
19
19
11
13
11
3
18
2
20
28
3
20
2
28
12
18
12
5
13
8
12
13
5
8
8
12
8
13
9
13
12
9
16
12
18
18
16
10
10
16
15
16
15
14
11
14
11
10
16
8
16
10
8
10
2
8
12
2
6
11
23
10
6
19
8
11
23
12
19
6
6
10
7
8
1
9
21
9
21
7
1
10
8
18
14
18
14
17
17
8
8
9
5
14
3
9
21
5
16
13
14
16
13
21
3
10
5
5
10
13
13
4
15
4
15
5
8
8
8
5
8
5
5
17
15
17
15
10
8
7
8
10
9
20
20
7
9
15
12
10
15
18
12
18
10
15
23
15
23
12
12
19
19
14
14
14
11
11
8
14
8
17
6
17
6
13
13
10
2
2
5
18
10
5
18
10
7
10
13
15
13
15
15
13
15
13
7
2
2
8
8
14
7
10
14
7
10
11
14
11
18
14
18
13
13
16
16
15
16
16
15
9
2
11
2
9
12
12
11
12
15
12
15
8
11
12
14
14
8
12
11
11
24
11
24
13
13
13
15
16
16
22
13
15
16
22
13
15
15
16
14
13
12
14
12
10
10
15
10
6
10
15
6
9
9
13
13
10
11
11
10
14
14
10
10
17
8
7
8
8
17
8
7
8
8
12
2
2
12
17
17
12
12
18
18
18
18
5
8
5
16
16
8
14
14
14
8
22
22
13
13
14
14
9
14
9
8
13
13
11
13
11
13
9
9
12
12
9
9
13
13
10
10
13
13
6
6
11
11
17
17
9
9
15
15
9
9
6
6
12
12
24
24
8
8
12
19
12
19
9
9
12
12

Model 1 Characteristic-specific molecular markers

Example 1 gene specific marker for herbicide tolerance introduced by genetic modification On the basis that [hellip] - there is verification of the reliability of the link between the marker and the characteristic - different markers for the same characteristic are different methods for examining the same characteristic [hellip]

Fusarium race 0 ex 1 Example Characteristic-specific molecular marker with incomplete information on state of expression

Model 1 Characteristic-specific molecular markers

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

ex Maize parent lines

Model 2 Combining phenotypic and molecular distances in the management of variety collections

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

2

02

Model 2 Combining phenotypic and molecular distances in the management of variety collections (continued)

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

13

New Model 3 Genetic selection of similar varieties for the first growing cycle

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
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0038
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0298
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0299
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03
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031
031
031
031
031
0311
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0319
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032
032
032
032
032
032
032
0321
0321
0321
0321
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0322
0322
0323
0323
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0324
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0324
0324
0324
0325
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0325
0326
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0327
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033
033
0331
0331
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0345
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035
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027
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027
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023
0283
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023
0314
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0149
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022
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0149
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028
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0287
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0314
026
017
0348
0349
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035
0272
0296
0293
0131
0123
0293
0336
034
0272
0123
0287
0333
0404
0132
0235
038
0266
0386
0352
0259
0291
026
0219
0351
0283
0238
0226
0251
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0298
0224
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027
0213
0038
0219
0067
0327
0298
0242
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0293
0329
0298
0286
0298
017
0219
0355
0309
0336
0303
0324
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0131
0186
0278
0278
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0114
0247
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0383
0116
02
0346
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0332
0313
0285
0316
0275
0319
0332
0405
0422
0345
0351
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018
0342
0255
0149
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0319
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0042
023
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0075
021
0345
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011
0252
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0115
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023
0281
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0389
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0312
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0149
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027
0278
0345
0068
0299
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025
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0213
0312
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0345
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028
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0038
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023
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0201
0045
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0219
0114
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029
0306
0042
0106
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0201
0225
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038
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0194
0367
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0045
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0235
0358
0342
0373
0296
0287
0386
0329
0329
018
0335
0361
0286
0339
0307
032
0334
0372
0405
0335
0345
0321
0364
0335
0316
0332
0338
0338
0345
0342
031
0295
0252
0352
0298
0336
0342
0269
0235
0279
0235
0284
0287
0329
0319
0312
0287
021
0304
0352
0324
0321
0336
037
0303
0287
0373
031
023
0283
0259
0286
0289
0255
0227
0262
0227
0264
0273
027
0304
0311
0273
0245
027
0289
0292
0295
0292
0312
0318
023
0283
0296
0295
023
0314
0291
0298
0325
0149
0295
0249
0276
022
0267
0273
0284
0329
0149
0239
028
0289
0298
0252
0342
0312
0282
0267
0325
0287
0252
0283
0314
026
017
0348
0349
0333
035
0272
0296
0293
0131
0123
0293
0336
034
0272
0123
0287
0333
0404
0132
0235
038
0266
0386
0352
0259
0291
026
0219
0351
0283
0238
0226
0251
0286
0298
0224
0262
0241
027
0213
0038
0219
0067
0327
0298
0242
0233
0203
0293
0329
0298
0286
0298
017
0219
0355
0309
0336
0303
0324
0306
0309
0131
0186
0278
0278
0312
0227
0114
0247
0306
0383
0116
02
0346
0259
0329
0336
0289
0325
0348
0351
0355
0332
0313
0285
0316
0275
0319
0332
0405
0422
0345
0351
0358
0348
0351
0275
0351
0364
0359
0364
0361
018
0342
0255
0149
0349
0283
0309
0332
0318
0279
0299
0238
0278
03
0319
0333
0068
0225
0281
0306
0309
0275
0327
0357
0281
0278
031
0335
0269
0227
0295
0333
0238
0336
0313
0318
0217
0228
0272
0306
0319
0329
0287
0299
0235
0236
0346
0269
0302
033
0376
0342
0176
0361
0361
0235
0262
0249
035
0226
0303
0285
0279
0217
026
0254
0269
0279
032
0341
0285
0217
0202
0294
0232
0254
0331
034
0285
0214
0356
0286
0279
0227
0276
0272
0251
0324
0316
0299
0228
026
0228
0262
0278
0286
0324
0312
0253
0246
0296
0281
0302
0306
0342
029
0281
0339
0339
0235
0264
022
0296
0286
0306
0275
0238
0272
0254
0228
0145
0268
0322
0361
025
0259
0275
029
0299
0185
0312
0323
0287
0312
0326
0307
0284
0273
0267
0293
0298
0309
0319
0278
0306
0269
0262
0145
0281
0316
0352
0284
0293
0288
0306
0315
0231
0309
032
0306
029
0339
032
0287
027
0273
0131
0224
0131
0332
03
0319
0279
0278
0268
0281
011
0288
0294
0281
0217
0042
023
0288
0377
0075
021
0345
0239
0334
0329
0304
0284
0123
0262
0186
0405
0319
0329
032
0286
0322
0316
011
0252
0309
0309
0249
0106
0276
0322
0412
0115
0219
0329
0276
0372
0319
0311
0329
0293
0241
0278
0422
0333
0287
0341
0324
0361
0352
0288
0252
0327
0293
023
0281
0253
0398
0389
0282
0284
0272
0342
0405
0312
0273
0149
0336
027
0278
0345
0068
0299
0285
0312
025
0284
0294
0309
0327
0231
0262
0287
0296
0269
0358
0342
029
0265
0304
0335
0287
0245
0239
034
0213
0312
0351
0225
0235
0217
0253
0259
0293
0281
0309
0293
0231
0198
0296
0219
0296
0299
0342
0306
0207
032
0345
021
027
028
0272
0038
0227
0358
0281
0236
0202
0246
0275
0288
0217
0249
023
0262
0198
0201
0045
0319
0313
0244
0236
0204
0285
0321
0304
0289
0289
0123
0219
0114
0348
0306
0346
0294
0296
029
0306
0042
0106
0281
0287
0296
0201
0225
0315
038
0063
0194
0367
0225
0364
0352
0292
0298
0287
0067
0247
0351
0309
0269
0232
0281
0299
0315
023
0276
0253
0296
0219
0045
0225
0299
0327
0251
0232
0228
031
0335
0324
0295
0252
0333
0327
0306
0275
0275
0302
0254
0302
0185
0231
0288
0322
0398
0269
0296
0319
0315
0299
0361
0339
0294
0318
0354
0316
0321
0292
0342
0404
0298
0383
0351
0327
033
0331
0306
0312
0309
0377
0412
0389
0358
0299
0313
038
0327
0361
0395
0368
0272
0389
0332
0336
0312
0312
0132
0242
0116
0364
0357
0376
034
0342
0323
032
0075
0115
0282
0342
0342
0244
0063
0251
0339
0395
0199
0361
0232
0338
037
0318
0282
0235
0233
02
0359
0281
0342
0285
029
0287
0306
021
0219
0284
029
0306
0236
0194
0232
0294
0368
0199
0342
0235
0338
0303
023
0267
038
0203
0346
0364
0278
0176
0214
0281
0312
029
0345
0329
0272
0265
0207
0204
0367
0228
0318
0272
0361
0342
0358
0345
0287
0283
0325
0266
0293
0259
0361
031
0361
0356
0339
0326
0339
0239
0276
0342
0304
032
0285
0225
031
0354
0389
0232
0235
0358
0342
0373
0296
0287
0386
0329
0329
018
0335
0361
0286
0339
0307
032
0334
0372
0405
0335
0345
0321
0364
0335
0316
0332
0338
0338
0345
0342
031
0295
0252
0352
0298
0336
0342
0269
0235
0279
0235
0284
0287
0329
0319
0312
0287
021
0304
0352
0324
0321
0336
037
0303
0287
0373
031
Page 11: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

Graph98

Type 3
Type 1
Type 4
Type 2
Express - Lady
Duetol - Tapidor
Eurol - Zorro
Ceres - Cheyenne
Express - Zorro
Zorro - Apex
Goeland - Lady
Apex - Goeland
Apex - Lady
Eurol - Idol
Bristol - Idol
Andol - Idol
Bristol - Eurol
Apex - Express
Bristol - Prestol
Andol - Eurol
Express - Goeland
Rogers Distances (Molecular marker distances)
GAIumlA Distances(Traditional characteristic distances)
GAIumlA Distances = f(Rogers Distances) for 28 varieties in the reference collectionTests 19971998
24
10
12
28
7
12
16
24
14
19
19
18
18
17
11
20
12
19
17
23
6
28
27
17
17
14
17
24
19
15
15
30
11
13
29
30
20
18
20
15
12
24
25
18
17
15
20
29
22
19
13
20
14
17
10
19
8
8
25
9
10
21
24
26
15
12
5
15
9
20
9
1
11
24
15
17
15
9
24
17
11
12
15
8
10
23
7
6
22
21
24
9
11
8
2
10
24
1
7
1
20
15
19
19
8
12
18
5
28
15
8
10
32
14
10
15
32
27
11
9
2
8
16
29
10
1
7
22
29
5
7
10
12
25
8
7
30
25
23
32
25
28
31
17
18
21
30
27
27
20
34
23
26
25
24
8
29
37
30
17
24
25
12
11
9
7
14
25
4
21
22
18
8
18
11
12
11
19
10
14
10
20
18
24
17
10
18
10
13
16
13
10
6
10
28
4
17
32
19
6
10
7
7
16
25
9
6
6
20
21
16
19
7
14
15
9
24
29
21
22
15
31
21
17
28
19
7
20
21
21
20
30
25
21
23
26
34
22
26
24
11
23
19
14
30
24
21
32
17
22
32
28
16
23
29
24
24
23
32
22
24
23
14
14
30
29
30
16
14
18
19
20
26
24
27
18
18
19
19
16
15
21
22
27
31
30
29
26
28
16
21
24
27
27
10
8
20
19
18
15
9
11
21
8
6
7
23
15
7
9
10
16
24
11
10
8
14
25
16
17
9
3
14
9
18
20
12
11
9
30
18
10
20
29
21
7
7
11
23
23
16
11
12
18
22
10
17
11
13
21
7
18
15
5
8
2
27
11
7
21
24
22
9
7
12
16
22
9
1
7
17
22
8
11
9
11
16
4
17
12
15
2
8
27
12
7
21
24
27
10
11
12
12
27
5
10
1
18
21
13
17
15
8
22
4
11
24
9
10
16
20
11
16
20
23
31
16
23
16
12
16
7
13
9
29
12
25
22
11
21
24
15
20
25
20
24
29
34
19
25
30
32
30
24
23
22
27
16
19
22
22
33
26
35
32
8
32
18
26
12
18
9
1
10
23
10
9
25
22
29
11
16
9
5
7
19
8
0
25
14
21
25
10
14
20
8
19
17
1
7
1
26
14
6
21
24
26
10
11
1
10
13
22
8
8
20
22
12
14
12
16
20
7
17
15
11
1
7
25
10
6
23
23
28
8
12
7
1
9
22
0
8
22
20
19
19
9
11
21
6
23
20
24
20
22
24
20
20
26
14
16
14
18
17
18
29
33
25
20
22
25
22
21
25
13
16
17
6
29
15
15
29
8
18
21
34
14
21
25
22
22
21
12
26
14
22
20
25
26
30
22
23
19
19
28
22
17
19
5
29
24
16
22
30
24
16
10
8
13
25
35
21
12
19
22
26
7
23
14
27
13
27
19
15
19
7
37
17
19
26
29
27
17
17
11
17
22
32
25
14
19
21
30
7
20
16
23
12
17
13
9
8
10
30
10
7
24
30
27
9
11
9
15
11
8
10
12
9
25
22
23
20
22
13
13
17
20
24
12
12
17
18
14
11
16
10
3
13
11
8
21
32
14
16
11
13
23
14
16
22
20
9
14
14
17
18
25
24
10
15
23
14
8
14
21
16
22
24
18
20
20
21
16
19
27
23
13
20
17
17
17
11
5
8
25
13
9
19
18
20
9
7
4
4
15
26
8
7
6
17
19
13
12
13
9
17

Graph99

169
677
61 20 varieacuteteacutes preacutesentes
93 19 varieacuteteacutes preacutesentes
Goeland-Lady
Goeland-Apex
Envol-Idol
Apex-Lady
Ceres-Falcon
Bristol-Eurol
Apex-Envol
Andol-Eurol
Eurol-Idol
Bristol-Prestol
Doublol-Duetol
Capitol-Vivol
Eurol-Samourai
Bristol-Samourai
Akamar-Duetol
Contact-Envol
Duetol-Navajo
Apex-Contact
Prestol-Samouraiet Goeland-Idol
Andol-Zorro
Falcon-Navajo
Andol-Falcon
Falcon-Goeland
Lady-Navajo
Duetol-Lady
Express-Zorro
Contact-Goeland
Duetol-Lady
Distances de ROGERS
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19981999
1
1
1
1
0
0
11
11
1
1
2
2
13
13
5
5
8
8
8
8
6
6
5
5
3
7
3
7
8
10
8
10
8
8
5
5
15
15
15
15
6
6
6
6
5
5
13
13
1
1
14
14
18
18
3
3
2
2
3
3
15
15
12
12
13
13
8
8
12
18
12
18
17
17
20
20
12
12
24
2
24
2
5
20
2
20
5
11
2
11
13
13
1
1
20
20
3
16
3
16
15
15
2
8
8
2
8
8
11
11
12
12
15
15
2
4
4
2
8
13
8
13
17
19
19
17
13
13
11
14
11
14
13
13
12
17
8
12
7
20
20
7
17
8
8
8
11
11
9
9
9
9
12
18
12
18
8
8
15
15
15
15
10
10
5
5
8
8
20
6
6
20
13
13
2
15
15
2
6
6
10
10
16
16
14
6
14
6
15
21
15
21
7
7
21
21
20
7
7
20
15
15
7
7
14
6
6
9
2
2
14
9
10
10
16
16
15
15
13
6
13
6
11
11
19
8
10
10
22
8
22
19
5
5
4
5
4
5
5
7
5
15
5
12
15
12
7
5
0
12
6
0
6
12
22
11
14
14
13
22
11
13
10
6
20
6
20
10
4
15
1
4
1
8
11
15
8
11
18
18
13
15
13
15
13
13
5
17
5
9
3
3
15
12
15
5
12
5
17
9
12
12
14
14
8
10
13
10
13
8
23
6
12
9
6
9
12
23
14
12
10
12
14
10
14
14
16
12
13
12
10
13
10
16
12
15
13
12
9
10
9
5
15
13
2
5
2
13
10
13
4
9
17
9
4
17
10
12
9
10
12
9
16
8
8
12
8
16
12
8
13
13
6
3
6
3
11
12
3
20
12
20
14
11
3
14
18
7
7
18
19
19
13
19
19
11
13
11
3
18
2
20
28
3
20
2
28
12
18
12
5
13
8
12
13
5
8
8
12
8
13
9
13
12
9
16
12
18
18
16
10
10
16
15
16
15
14
11
14
11
10
16
8
16
10
8
10
2
8
12
2
6
11
23
10
6
19
8
11
23
12
19
6
6
10
7
8
1
9
21
9
21
7
1
10
8
18
14
18
14
17
17
8
8
9
5
14
3
9
21
5
16
13
14
16
13
21
3
10
5
5
10
13
13
4
15
4
15
5
8
8
8
5
8
5
5
17
15
17
15
10
8
7
8
10
9
20
20
7
9
15
12
10
15
18
12
18
10
15
23
15
23
12
12
19
19
14
14
14
11
11
8
14
8
17
6
17
6
13
13
10
2
2
5
18
10
5
18
10
7
10
13
15
13
15
15
13
15
13
7
2
2
8
8
14
7
10
14
7
10
11
14
11
18
14
18
13
13
16
16
15
16
16
15
9
2
11
2
9
12
12
11
12
15
12
15
8
11
12
14
14
8
12
11
11
24
11
24
13
13
13
15
16
16
22
13
15
16
22
13
15
15
16
14
13
12
14
12
10
10
15
10
6
10
15
6
9
9
13
13
10
11
11
10
14
14
10
10
17
8
7
8
8
17
8
7
8
8
12
2
2
12
17
17
12
12
18
18
18
18
5
8
5
16
16
8
14
14
14
8
22
22
13
13
14
14
9
14
9
8
13
13
11
13
11
13
9
9
12
12
9
9
13
13
10
10
13
13
6
6
11
11
17
17
9
9
15
15
9
9
6
6
12
12
24
24
8
8
12
19
12
19
9
9
12
12

Model 1 Characteristic-specific molecular markers

Example 1 gene specific marker for herbicide tolerance introduced by genetic modification On the basis that [hellip] - there is verification of the reliability of the link between the marker and the characteristic - different markers for the same characteristic are different methods for examining the same characteristic [hellip]

Fusarium race 0 ex 1 Example Characteristic-specific molecular marker with incomplete information on state of expression

Model 1 Characteristic-specific molecular markers

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

ex Maize parent lines

Model 2 Combining phenotypic and molecular distances in the management of variety collections

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

2

02

Model 2 Combining phenotypic and molecular distances in the management of variety collections (continued)

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

13

New Model 3 Genetic selection of similar varieties for the first growing cycle

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
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0038
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0298
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03
0302
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031
031
031
0311
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0319
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032
032
032
032
032
032
032
0321
0321
0321
0321
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0322
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0323
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0324
0324
0324
0325
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0325
0326
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0327
0327
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033
033
0331
0331
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0345
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035
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023
0283
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023
0314
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0149
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022
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0149
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028
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0314
026
017
0348
0349
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035
0272
0296
0293
0131
0123
0293
0336
034
0272
0123
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0333
0404
0132
0235
038
0266
0386
0352
0259
0291
026
0219
0351
0283
0238
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0224
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027
0213
0038
0219
0067
0327
0298
0242
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0298
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0298
017
0219
0355
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0336
0303
0324
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0131
0186
0278
0278
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0114
0247
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0383
0116
02
0346
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0313
0285
0316
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0319
0332
0405
0422
0345
0351
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018
0342
0255
0149
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0319
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023
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0075
021
0345
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011
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0115
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023
0281
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0149
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0278
0345
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0213
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0345
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029
0306
0042
0106
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0201
0225
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038
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0045
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0352
0324
0321
0336
037
0303
0287
0373
031
Page 12: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

Graph99

169
677
61 20 varieacuteteacutes preacutesentes
93 19 varieacuteteacutes preacutesentes
Goeland-Lady
Goeland-Apex
Envol-Idol
Apex-Lady
Ceres-Falcon
Bristol-Eurol
Apex-Envol
Andol-Eurol
Eurol-Idol
Bristol-Prestol
Doublol-Duetol
Capitol-Vivol
Eurol-Samourai
Bristol-Samourai
Akamar-Duetol
Contact-Envol
Duetol-Navajo
Apex-Contact
Prestol-Samouraiet Goeland-Idol
Andol-Zorro
Falcon-Navajo
Andol-Falcon
Falcon-Goeland
Lady-Navajo
Duetol-Lady
Express-Zorro
Contact-Goeland
Duetol-Lady
Distances de ROGERS
Distances GAIumlA
Distances GAIumlA = f(Distances de Rogers) pour 28 varieacuteteacutes de la collection de reacutefeacuterence Essais 19981999
1
1
1
1
0
0
11
11
1
1
2
2
13
13
5
5
8
8
8
8
6
6
5
5
3
7
3
7
8
10
8
10
8
8
5
5
15
15
15
15
6
6
6
6
5
5
13
13
1
1
14
14
18
18
3
3
2
2
3
3
15
15
12
12
13
13
8
8
12
18
12
18
17
17
20
20
12
12
24
2
24
2
5
20
2
20
5
11
2
11
13
13
1
1
20
20
3
16
3
16
15
15
2
8
8
2
8
8
11
11
12
12
15
15
2
4
4
2
8
13
8
13
17
19
19
17
13
13
11
14
11
14
13
13
12
17
8
12
7
20
20
7
17
8
8
8
11
11
9
9
9
9
12
18
12
18
8
8
15
15
15
15
10
10
5
5
8
8
20
6
6
20
13
13
2
15
15
2
6
6
10
10
16
16
14
6
14
6
15
21
15
21
7
7
21
21
20
7
7
20
15
15
7
7
14
6
6
9
2
2
14
9
10
10
16
16
15
15
13
6
13
6
11
11
19
8
10
10
22
8
22
19
5
5
4
5
4
5
5
7
5
15
5
12
15
12
7
5
0
12
6
0
6
12
22
11
14
14
13
22
11
13
10
6
20
6
20
10
4
15
1
4
1
8
11
15
8
11
18
18
13
15
13
15
13
13
5
17
5
9
3
3
15
12
15
5
12
5
17
9
12
12
14
14
8
10
13
10
13
8
23
6
12
9
6
9
12
23
14
12
10
12
14
10
14
14
16
12
13
12
10
13
10
16
12
15
13
12
9
10
9
5
15
13
2
5
2
13
10
13
4
9
17
9
4
17
10
12
9
10
12
9
16
8
8
12
8
16
12
8
13
13
6
3
6
3
11
12
3
20
12
20
14
11
3
14
18
7
7
18
19
19
13
19
19
11
13
11
3
18
2
20
28
3
20
2
28
12
18
12
5
13
8
12
13
5
8
8
12
8
13
9
13
12
9
16
12
18
18
16
10
10
16
15
16
15
14
11
14
11
10
16
8
16
10
8
10
2
8
12
2
6
11
23
10
6
19
8
11
23
12
19
6
6
10
7
8
1
9
21
9
21
7
1
10
8
18
14
18
14
17
17
8
8
9
5
14
3
9
21
5
16
13
14
16
13
21
3
10
5
5
10
13
13
4
15
4
15
5
8
8
8
5
8
5
5
17
15
17
15
10
8
7
8
10
9
20
20
7
9
15
12
10
15
18
12
18
10
15
23
15
23
12
12
19
19
14
14
14
11
11
8
14
8
17
6
17
6
13
13
10
2
2
5
18
10
5
18
10
7
10
13
15
13
15
15
13
15
13
7
2
2
8
8
14
7
10
14
7
10
11
14
11
18
14
18
13
13
16
16
15
16
16
15
9
2
11
2
9
12
12
11
12
15
12
15
8
11
12
14
14
8
12
11
11
24
11
24
13
13
13
15
16
16
22
13
15
16
22
13
15
15
16
14
13
12
14
12
10
10
15
10
6
10
15
6
9
9
13
13
10
11
11
10
14
14
10
10
17
8
7
8
8
17
8
7
8
8
12
2
2
12
17
17
12
12
18
18
18
18
5
8
5
16
16
8
14
14
14
8
22
22
13
13
14
14
9
14
9
8
13
13
11
13
11
13
9
9
12
12
9
9
13
13
10
10
13
13
6
6
11
11
17
17
9
9
15
15
9
9
6
6
12
12
24
24
8
8
12
19
12
19
9
9
12
12

Model 1 Characteristic-specific molecular markers

Example 1 gene specific marker for herbicide tolerance introduced by genetic modification On the basis that [hellip] - there is verification of the reliability of the link between the marker and the characteristic - different markers for the same characteristic are different methods for examining the same characteristic [hellip]

Fusarium race 0 ex 1 Example Characteristic-specific molecular marker with incomplete information on state of expression

Model 1 Characteristic-specific molecular markers

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

ex Maize parent lines

Model 2 Combining phenotypic and molecular distances in the management of variety collections

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

2

02

Model 2 Combining phenotypic and molecular distances in the management of variety collections (continued)

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

13

New Model 3 Genetic selection of similar varieties for the first growing cycle

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
0038
0038
0042
0042
0045
0045
0063
0063
0067
0067
0068
0068
0075
0075
0106
0106
011
011
0114
0114
0115
0115
0116
0116
0123
0123
0123
0123
0131
0131
0131
0131
0132
0132
0145
0145
0149
0149
0149
0149
017
017
0176
0176
018
018
0185
0185
0186
0186
0194
0194
0198
0198
0199
0199
02
02
0201
0201
0202
0202
0203
0203
0204
0204
0207
0207
021
021
021
021
0213
0213
0214
0214
0217
0217
0217
0217
0217
0217
0219
0219
0219
0219
0219
0219
0219
0219
022
022
0224
0224
0225
0225
0225
0225
0225
0225
0226
0226
0227
0227
0227
0227
0227
0227
0228
0228
0228
0228
0228
0228
023
023
023
023
023
023
023
023
0231
0231
0231
0231
0232
0232
0232
0232
0232
0232
0233
0233
0235
0235
0235
0235
0235
0235
0235
0235
0235
0235
0236
0236
0236
0236
0238
0238
0238
0238
0239
0239
0239
0239
0241
0241
0242
0242
0244
0244
0245
0245
0246
0246
0247
0247
0249
0249
0249
0249
025
025
0251
0251
0251
0251
0252
0252
0252
0252
0252
0252
0253
0253
0253
0253
0254
0254
0254
0254
0255
0255
0259
0259
0259
0259
0259
0259
026
026
026
026
0262
0262
0262
0262
0262
0262
0262
0262
0264
0264
0265
0265
0266
0266
0267
0267
0267
0267
0268
0268
0269
0269
0269
0269
0269
0269
0269
0269
027
027
027
027
027
027
0272
0272
0272
0272
0272
0272
0272
0272
0272
0272
0273
0273
0273
0273
0273
0273
0275
0275
0275
0275
0275
0275
0275
0275
0276
0276
0276
0276
0276
0276
0278
0278
0278
0278
0278
0278
0278
0278
0278
0278
0279
0279
0279
0279
0279
0279
028
028
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0281
0282
0282
0282
0282
0283
0283
0283
0283
0283
0283
0284
0284
0284
0284
0284
0284
0284
0284
0285
0285
0285
0285
0285
0285
0285
0285
0286
0286
0286
0286
0286
0286
0286
0286
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0287
0288
0288
0288
0288
0288
0288
0289
0289
0289
0289
0289
0289
029
029
029
029
029
029
029
029
0291
0291
0292
0292
0292
0292
0293
0293
0293
0293
0293
0293
0293
0293
0293
0293
0294
0294
0294
0294
0294
0294
0295
0295
0295
0295
0295
0295
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0296
0298
0298
0298
0298
0298
0298
0298
0298
0298
0298
0299
0299
0299
0299
0299
0299
0299
0299
0299
0299
03
03
0302
0302
0302
0302
0303
0303
0303
0303
0304
0304
0304
0304
0304
0304
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0306
0307
0307
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
0309
031
031
031
031
031
031
0311
0311
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0312
0313
0313
0313
0313
0314
0314
0315
0315
0315
0315
0316
0316
0316
0316
0316
0316
0318
0318
0318
0318
0318
0318
0319
0319
0319
0319
0319
0319
0319
0319
0319
0319
032
032
032
032
032
032
032
032
0321
0321
0321
0321
0322
0322
0322
0322
0323
0323
0324
0324
0324
0324
0324
0324
0325
0325
0325
0325
0326
0326
0327
0327
0327
0327
0327
0327
0327
0327
0329
0329
0329
0329
0329
0329
0329
0329
0329
0329
0329
0329
033
033
0331
0331
0332
0332
0332
0332
0332
0332
0333
0333
0333
0333
0333
0333
0334
0334
0335
0335
0335
0335
0335
0335
0336
0336
0336
0336
0336
0336
0336
0336
0338
0338
0338
0338
0339
0339
0339
0339
0339
0339
0339
0339
034
034
034
034
0341
0341
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0342
0345
0345
0345
0345
0345
0345
0345
0345
0346
0346
0346
0346
0348
0348
0348
0348
0349
0349
035
035
0351
0351
0351
0351
0351
0351
0351
0351
0352
0352
0352
0352
0352
0352
0354
0354
0355
0355
0356
0356
0357
0357
0358
0358
0358
0358
0358
0358
0359
0359
0361
0361
0361
0361
0361
0361
0361
0361
0361
0361
0361
0361
0364
0364
0364
0364
0364
0364
0367
0367
0368
0368
037
037
0372
0372
0373
0373
0376
0376
0377
0377
038
038
038
038
0383
0383
0386
0386
0389
0389
0389
0389
0395
0395
0398
0398
0404
0404
0405
0405
0405
0405
0412
0412
0422
0422
Page 13: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

Model 1 Characteristic-specific molecular markers

Example 1 gene specific marker for herbicide tolerance introduced by genetic modification On the basis that [hellip] - there is verification of the reliability of the link between the marker and the characteristic - different markers for the same characteristic are different methods for examining the same characteristic [hellip]

Fusarium race 0 ex 1 Example Characteristic-specific molecular marker with incomplete information on state of expression

Model 1 Characteristic-specific molecular markers

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

ex Maize parent lines

Model 2 Combining phenotypic and molecular distances in the management of variety collections

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

2

02

Model 2 Combining phenotypic and molecular distances in the management of variety collections (continued)

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

13

New Model 3 Genetic selection of similar varieties for the first growing cycle

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 14: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

Fusarium race 0 ex 1 Example Characteristic-specific molecular marker with incomplete information on state of expression

Model 1 Characteristic-specific molecular markers

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

ex Maize parent lines

Model 2 Combining phenotypic and molecular distances in the management of variety collections

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

2

02

Model 2 Combining phenotypic and molecular distances in the management of variety collections (continued)

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

13

New Model 3 Genetic selection of similar varieties for the first growing cycle

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 15: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

ex Maize parent lines

Model 2 Combining phenotypic and molecular distances in the management of variety collections

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

2

02

Model 2 Combining phenotypic and molecular distances in the management of variety collections (continued)

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

13

New Model 3 Genetic selection of similar varieties for the first growing cycle

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 16: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

04030201

GAIA

Rogers

1

2

3

4

5

6

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological

data

Threshold for molecular distances

2

02

Model 2 Combining phenotypic and molecular distances in the management of variety collections (continued)

21

PROPOSED USE OF MOLECULAR AND MORPHOLOGICAL DATA

04

03

02

01

GAIA

Rogers

1

2

3

4

5

6

To put in the field

To put in the field

laquo Distinct plus raquo varieties

laquo Distinct plus raquo varieties

Threshold for morphological data

Threshold for molecular distances

21

Commenter en ligne

Drsquoabord la ligne du haut

Puis la ligne du bas

Puis la ligne du milieu

13

New Model 3 Genetic selection of similar varieties for the first growing cycle

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 17: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

13

New Model 3 Genetic selection of similar varieties for the first growing cycle

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 18: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

New Model 3 Genetic selection of similar varieties for the first growing cycle (continued)

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 19: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

bull For a variety to be protected it needs to be clearly distinguishable from all existing varieties on the basis of characteristics that are physically expressed eg plant height time of flowering fruit color disease resistance etc

bull The DNA-profile is not the basis for obtaining the protection of a variety although this information may be used as supporting information

bull A more detailed explanation is provided in the FAQ Does UPOV allow molecular techniques (DNA profiles) in the examination of Distinctness Uniformity and Stability (ldquoDUSrdquo)

15

Is it possible to obtain protection of a variety on the basis of its DNA-profile

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 20: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination

bull It is important to note that in some cases varieties may have a different DNA profile but be phenotypically identical whilst in other cases varieties which have a large phenotypic difference may have the same DNA profile for a particular set of molecular markers (eg some mutations)

bull In relation to the use of molecular markers that are not related to phenotypic differences the concern is that it might be possible to use a limitless number of markers to find differences between varieties at the genetic level that are not reflected in phenotypic characteristics

On the above basis UPOV has agreed the following uses in relation to DUS examination

16

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 21: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

(a)Molecular markers can be used as a method of examining DUS characteristics that satisfy the criteria for characteristics set out in the General Introduction if there is a reliable link between the marker and the characteristic

(b)A combination of phenotypic differences and molecular distances can be used to improve the selection of varieties to be compared in the growing trial if the molecular distances are sufficiently related to phenotypic differences and the method does not create an increased risk of not selecting a variety in the variety collection which should be compared to candidate varieties in the DUS growing trial

17

Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination (Contrsquod)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 22: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 23: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

WORKSHOP 1 (UPOV BMT14 - Seoul Republic of Korea November 12 2014)

Agreed 1 would be useful to develop a joint document explaining the principal features (eg

DUS variety identification variety purity etc ) of the systems of OECD UPOV and ISTA

OECDUPOVISTA JOINT WORKSHOP ON MOLECULAR TECHNIQUES

WORKSHOP 2

(OECD Seed Schemes- Paris June 8 2016) OECD Annual Meeting approved 1 develop a joint document explaining the principal features (eg DUS variety

identification variety purity etc ) of the systems of OECD UPOV AOSA and ISTA

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 24: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

WORKSHOP 1

Agreed 2 to propose an inventory by UPOV OECD and ISTA of the use of molecular marker

techniques by crop with a view to developing a document containing that information in a similar format to UPOV document UPOVINF16 ldquoExchangeable Softwarerdquo

WORKSHOP 2

OECD Annual Meeting approved 2 to carry out a joint inventory by UPOV OECD AOSA and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information The OECD will contribute to the document by sharing the ongoing list of molecular techniques used by NDAs and continuously collected by the Secretariat

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 25: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

WORKSHOP 1

Agreed 3 to propose to invite UPOV OECD and ISTA to develop lists of possible joint

initiatives in relation to molecular techniques It was noted that in the case of UPOV the list could be drafted by the BMT at its fifteenth session subject to approval by the Technical Committee bull The UPOV TC at its fifty-second session agreed that the BMT should include the

development of a list of terminology (definitions) used by OECD UPOV and ISTA in the list of joint initiatives

bull The UPOV BMT at its fifteenth session agreed that possible future collaboration between UPOV OECD and ISTA might include the harmonization of terms and methodologies used for different crops and the possible development of standards after agreement by these organizations

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 26: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

WORKSHOP 2

OECD Annual Meeting approved 3 To develop a list of terms and their definitions as used by OECD UPOV AOSA and ISTA

and to make an attempt to harmonise these

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 27: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

WORKSHOP 1

Agreed 4 would be useful for mutual understanding to repeat the joint workshop at relevant

meetings of the OECD and ISTA

WORKSHOP 2

OECD Annual Meeting approved 4 to consider organising another similar workshop [in OECD] in three years time

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 28: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

for consideration 1 to develop a joint document explaining the principal features of the systems

of OECD UPOV and ISTA (eg DUS variety identification variety purity etc ) 2 To develop an inventory by UPOV OECD and ISTA of the use of molecular

marker techniques by crop with a view to developing a document containing that information

3 to develop lists of possible joint initiatives in relation to molecular techniques (eg the development of a list of terminology (definitions) used by OECD UPOV and ISTA)

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 29: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

Preview

bull Introduction ndash purpose of use of BMTs in UPOV bull Principles for BMTs use in UPOV (ldquomodelsrdquo) bull Cooperation with international organizations bull Next steps

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 30: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

Next Steps

bull The TC agreed that UPOV and OECD should make progress on the matters previously agreed

bull The TC agreed to invite ISTA to join the initiatives when in a position to do so

bull UPOV Survey inventory on the use of molecular marker techniques by crop under preparation

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
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  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
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Page 31: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

The BMT is a group open to DUS experts biochemical and molecular specialists and plant breeders whose role is to bull Review general developments in biochemical and molecular techniques bull Maintain an awareness of relevant applications of biochemical and molecular techniques in

plant breeding bull Consider the possible application of biochemical and molecular techniques in DUS testing

and report its considerations to the TC bull [hellip] bull Provide a forum for discussion on the use of biochemical and molecular techniques in the

consideration of essential derivation and variety identification

29

Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
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  • Fusarium race 0 ex 1
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  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30
Page 32: UPOV Guidance on Molecular Techniques - seedtest.org · Hyderabad, India, June 29, 2019 UPOV Guidance on Molecular Techniques . Leontino Taveira Head of Technical Affairs and Regional

Next meeting of the UPOV BMT

October 16 to October 18 2019 (Hangzhou China)

All invited

  • Hyderabad India June 29 2019
  • Preview
  • Slide Number 3
  • Importance of Harmonized Approach within UPOV
  • Preview
  • Slide Number 6
  • Slide Number 7
  • Calibrated molecular distances in the management of variety collections
  • Slide Number 9
  • Fusarium race 0 ex 1
  • Slide Number 11
  • Slide Number 12
  • Slide Number 13
  • Slide Number 14
  • Slide Number 15
  • Question Does UPOV allow molecular techniques (DNA profiles) in the DUS examination
  • Slide Number 17
  • Preview
  • Slide Number 19
  • Slide Number 20
  • Slide Number 21
  • Slide Number 22
  • Slide Number 23
  • Slide Number 26
  • Preview
  • Next Steps
  • Working Group on Biochemical and Molecular Techniques and DNA-Profiling in Particular
  • Slide Number 30

FINAL REPORT A POTENTIAL UPOV OPTION 2 APPROACH FOR …cpvo.europa.eu/sites/default/files/documents/tech... · of varieties to be included in a growing test can be reduced. UPOV TG/1/3

Overview of the UPOV Convention - Food and Agriculture ... · 4 7 UPOV MISSION STATEMENT “To provide and promote an effective system of plant variety protection (PVP), […]”

The UPOV Convention, Farmers Rights and human rights study_en_short.pdf · developing national plant variety protection (PVP) laws, often based on the UPOV system. •NGOs and farmer

UPOV mission for enhancing the effectiveness of the PVP …eapvp.org/files/report/docs/01. UPOV_Jun Koide.pdf•INF/12: “Explanatory Notes on Variety Denominations” •UPOV Code

Session 6 0 Upov Lavignolle

Developments concerning UPOV PRISMA Focus on Latin …...UPOV PRISMA (e.g. proof of payment) • Train PVP Officers (use of the Tool) • Communicate/ publicize UPOV PRISMA with your

Nuno Taveira ENERCON - · PDF fileNuno Taveira ENERCON GmbH Los códigos de red en sistemas aislados, consecuencia para los fabricantes Grid codes in isolated systems, consequences

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Comments of UPOV January 23, 2009 · Comments of UPOV January 23, 2009 ... January 2009. 2 CONTENT 1. Introduction 2. Overview and factual description of the relevant ABS provisions

FINAL REPORT A POTENTIAL UPOV OPTION 2 ......of varieties to be included in a growing test can be reduced. UPOV TG/1/3 (2002) allows that ‘a systematic individual comparison may

User Guide for Agents · User Guide for Agents UPOV PRISMA Version 2.4 . Page 2 of 81 Contents 1 About this guide _____ 3 2 Acronyms _____ 3 ... Agents _____ 79 . UPOV PRISMA User

  · Web viewUPOV/WG-DEN/5/2. ANNEX VII. UPOV/WG-DEN/5/2. ANNEX I. UPOV/WG-DEN/5/2. ANNEX V. UPOV/WG-DEN/5/2. Annex I, page 38. UPOV/WG-DEN/5/2. ANNEX VIII. UPOV/WG-DEN/5/2. Annex

INESC-ID/INOV Joao P. Taveira - datatracker.ietf.org · Expires: January 5, 2015 Mario S. Nunes INESC-ID/INOV Joao P. Taveira IST/INOV Lingli Deng China Mobile July 4, 2014 PPSP Tracker

Introduction to UPOV/ Role of PVP in Technology Transfer ...eapvp.org/files/report/docs/vietnam/1_Introduction to UPOV.pdfExplanatory notes UPOV/EXN/ENF/ 1 . UPOV Convention requires

The UPOV sui generis system for the protection of plant ...isopb.mpob.gov.my/pdfFile/2nd/PAPER 17 Gasselin UPOV.pdf · 27-28/11/2006 ISOPB : UPOV System and Oil Palm H. Feyt & T

UPOV morphological versus molecular markers for maize ... · PDF fileINTRODUCTION Maize (Zea mays L.) ... maize breeding. The objectives of this study were (i) to compare similarities

The UPOV Convention, Farmers Rights and human rights · lations between the UPOV Conven-tion, Farmers Z Rights and human rights. •Many countries are on the way to developing national

TGP/7/1 - International Union for the Protection of New ... · Web view[Readers are advised to consult the UPOV Code, which can be found on the UPOV Website ( ), for the latest information.]

The UPOV Convention: an International Standard · 11.10.2011  · 1 Brussels, October 11, 2011 The UPOV Convention: an International Standard Peter Button (Vice Secretary-General,

The relationship between the IR, the WTO, UPOV … · The relationship between the IR, the WTO, UPOV Convention and WIPO Jorge Cabrera Medaglia. Structure of the study 1. Factual

Seminar on Awareness Raising on the UPOV System of Plant

  · Web view2014-09-10 · DRAFT (REVISION) Note: a further review of document UPOV/INF/12 might be necessary in relation to the development of an effective UPOV similarity search

UPOV and CPVOcpvo.europa.eu/.../documents/News/UPOV_presentation... · UPOV should consider the development of an electronic version of the UPOV model application form and technical

Rolf Jördens, Vice Secretary-General, UPOV Geneva, May 29, 2007

Developments in UPOVof OECD, UPOV and ISTA (e.g. DUS, variety identification, variety purity, etc. ) 2. To develop an inventory by UPOV, OECD and ISTA of the use of molecular marker

Working Group on Biochemical and Molecular Techniques BMT ... fileThe BMT agreed to propose that paragraph 3.1.4(reproduced below) from document UPOV/INF/18/1 be introduced in document

REPORT ON DEVELOPMENTS IN UPOV · Annex, page 4 The boundaries shown on this map do not imply the expression of any opinion whatsoever on the part of UPOV concerning the legal status

Management of lymphangioleiomyomatosisf1000researchdata.s3.amazonaws.com/f1000reports/files/9008/6/11… · Management of lymphangioleiomyomatosis Angelo M. Taveira-DaSilva* and Joel

Sistem PVT di UPOV member dan Non UPOV Member countriespvtpp.setjen.pertanian.go.id/cms/wp-content/uploads/2018/01/Sistem... · Sistem PVT di UPOV member dan Non UPOV Member countries

Plant Breeders’ Rights and theafsta.org/reload/wp-content/uploads/2018/03/session-4-Peter-UPOV-rev.pdfWFO welcomes the 1991 UPOV Convention as: It is an inter-governmental platform

UPOV morphological versus molecular markers for …In maize (Zea mays L.) breeding programs, it is very important to define a genetic distance of inbred lines and to establish criteria

PLANT VARIETY PROTECTION - International Union for the ... · Plant Variety Protection is a UPOV publication that reports on national and international events in ... (SNICS) UPOV

Working Group on Biochemical and Molecular …upov.int/edocs/mdocs/upov/en/bmt_16/bmt_16_5_add.pdfAntibiotic resstance profiÞs Fatty acid and q profiles Hybridization blots Bioch

08398 - DETECTION OF CORROSION OF POST …sagues/Documents/Nace08 398 Taveira PT Corr P14… · DETECTION OF CORROSION OF POST-TENSIONED STRANDS IN GROUTED ASSEMBLIES L. V. Taveira,