Welcome to 2nd Kick-off

Meeting Project Resistvir

7-8 July 2005Ankara / TURKEY

Ankara University Ankara University The Faculty of The Faculty of

AgricultureAgriculture

Ankara University Ankara University The Faculty of The Faculty of Agriculture Agriculture

Department of Plant Department of Plant ProtectionProtection

Virology Virology DepartmentDepartment

1 Prof. Dr. 1 Prof. Dr. 1 Dr. Res. Asist.1 Dr. Res. Asist.

1 Ph.D. Res. Asist.1 Ph.D. Res. Asist.1 Ph.D. Student1 Ph.D. Student1 M.Sc. Student1 M.Sc. Student

Our Important Our Important Research AreasResearch Areas•Vegetable Crop VirusesVegetable Crop Viruses

Cucumber Mosaic Cucumovirus (CMV)Zucchini Yellow Mosaic Potyvirus (ZYMV)

Sugarbeet VirusSugarbeet Virus Beet Necrotic Yellow Vein

Benyvirus (BNYVV)(Rhizomania)

•Wheat VirusesWheat VirusesBarley Stripe Mosaic Virus (BSMV) Barley Stripe Mosaic Virus (BSMV) Wheat Soil-borne Mosaic Virus (WSbMV) Wheat Soil-borne Mosaic Virus (WSbMV) (BSMV)(BSMV)

RNA 5 in Turkish isolates. M Marker ( DNA/Eco RI+Hind III); lane 1 isolate no. M-2; lane 2 isolate no. I-2; lane 3 isolate no. Ad-2; lane 4 isolate no. A-5; lane 5 isolate no. A-30; lane 6 isolate no. A-32; lane 7 isolate no. Kr-1; lane 8 isolate no. Kr-2; lane 9 isolate no. Kr-3; lane 10 isolate no. K-4; lane 11 isolate no. K-5; lane 12 isolate no. K-8; lane 13 isolate no. K-11; lane 14 isolate no. Ky-6; lane 15 isolate no. Ky-14; lane 16 isolate no. Ky-15; lane 17 isolate no. Ky-22; lane 18 isolate no. A-12; lane 19 isolate no. Al-2.

21226 bp

947 bp

M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

1000 bp

PDV

PNRSVPNRSV

PDV

Fruit VirusesFruit VirusesStone Fruit Stone Fruit

VirusesViruses

PPV PPV PPV PPV PPV PPV PPV PPV

PPVPPV

Reverse transcription polymerase chain reaction (RT-PCR) analysis of some Turkish Prunus necrotic ringspot virus (PNRSV) isolates. M, lambda DNA/EcoRI + HindIII Marker 3 (MBI Fermentas). PCR product 616 bp

RT-PCR results of PDV infected samples using a) #PD1 ve #PD2 primers b) #PD3 and #PD4 primers (3 Macar8; 5 Hatay50; 7 Pozantı2; 10 Pozantı4; 11 Ömerli7; 12 Ömerli8; 14 Yalova48; 15 Maraş; 16 negatif RNA;17 PDV plasmid; 18 ddH2O control)

a

b

Pome Fruit Pome Fruit VirusesViruses

(New started)

(ApMV)(ApMV)

((ACLSVACLSV))

(ASGV)(ASGV)

(ASPV)(ASPV)

Grapevine leaf roll associated virus

Grapevine fanleaf virus

Grapevine VirusesGrapevine Viruses

Molecular Biology Laboratory

Serology Laboratory

2 Project Newly Submitted

1 Ministry of Agriculture and Rural Affairs2 The Scientific & Technical Research Council of Turkey (TUBITAK)3 Research Fund of Ankara University1 DPT4 Ankara University Biotechnology Research Center

Totally 11 Project Completed

Resistvir ; This is The First Resistvir ; This is The First EU project of our laboratory, EU project of our laboratory, Formerly, our mycologists Formerly, our mycologists were included in an EU were included in an EU project on Chickpea blight, project on Chickpea blight, Ascochyta rabiei at EU5 th Ascochyta rabiei at EU5 th Frame work, it has been Frame work, it has been concluded at 2004concluded at 2004