9 native-page法による絶滅危惧植物分類の試み
TRANSCRIPT
2
3
DNA
95 Sigma-Aldorich pH 10
99
4B29cm 8cm1235g710 31cm2770g10617cm 464g
4B1345cmA 12720g7 A2241cm6540g 101237cm3420g
1013cm 10B
9363 A57cmB 79cm 3.57.0g
464 1223cm2350g
536.5cm7 57.5cm
72.5g
5 4
2 2.31278
10
3
4a 100 46 111745
5 0.40.5m
6
7
10mA 3020mA
37
2 GOT
LAP SKDH ADH LDH MDH
G6PDH6
FDH GDH
4
Rm
1GOT GOT . AC
A 2002
B 2003
101
4 mol/l)M 4 s t a c k i n g buffer(50ml) A 1M HC1 48ml
Trizmabase 36.6g TEMED 0.23ml
Trizmabase 5.98g 100ml C 29.2g
BIS 0.8g 100ml F
Trizmabase 6.0g 28.8g
1000ml pH8.3 G 0.14g 100ml G' 10 g 9ml
1000ml) reservoirbuffer pH4.3 0.2M PHospHate bufferpH 7 .5 )
0.1M Tris-HCI pH8.0 0.2M Tris-HCI pH8.0 0.1M Tris-HCI pH7.0 0.2M L-L-Asparticacid 2M KOH 4.0 L-Ieucyl-B-napHthylamide 1M DL- 2M NaOH 1.0Magnesium chloride 6
0.1BPB) 0.2M Tris-0.2M Maleate,pH3.8 NAD
NADP
5 20
1530μl
10mA 3020mA
37
2 GOT
LAP SKDH ADH LDH MDH
G6PDH6
FDH GDH
4
Rm
1GOT GOT . AC
A 2002
B 2003
101
4 mol/l)M 4 s t a c k i n g buffer(50ml) A 1M HC1 48ml
Trizmabase 36.6g TEMED 0.23ml
Trizmabase 5.98g 100ml C 29.2g
BIS 0.8g 100ml
G' 10 g 9ml
1000ml) reservoirbuffer
pH4.3 0.2M PHospHate bufferpH 7 .5 )
0.1M Tris-HCI pH8.0 0.2M Tris-HCI pH8.0 0.1M Tris-HCI pH7.0 0.2M L-L-Asparticacid 2M KOH 4.0 L-Ieucyl-B-napHthylamide 1M DL- 2M NaOH 1.0Magnesium chloride 6
0.1BPB) 0.2M Tris-0.2M Maleate,pH3.8 NAD
NADP
5 20
1530μl
7GPDHFDHLDH GPDHFDHLDH
8 NativePAGE
5
1
2MDH GDH
3
103
e100GOT GOT GOTGOT
2ADH ADH ADH
E ADH1
fastF
3MDH MDH
4SKDH SKDHF
4F
5GDH GDH 2 GDH1
6LAP
7GPDHFDHLDH GPDHFDHLDH
8 NativePAGE
5
1
2MDH GDH
3
103
2ADH ADH
ADH
E ADH1
fastF
3MDH MDH
4SKDH SKDHF
4F
5GDH GDH 2 GDH1
6LAP
3
DNA
95 Sigma-Aldorich pH 10
99
4B29cm 8cm1235g710 31cm2770g10617cm 464g
4B1345cmA 12720g7 A2241cm6540g 101237cm3420g
1013cm 10B
9363 A57cmB 79cm 3.57.0g
464 1223cm2350g
536.5cm7 57.5cm
72.5g
5 4
2 2.31278
10
3
4a 100 46 111745
5 0.40.5m
6
7
10mA 3020mA
37
2 GOT
LAP SKDH ADH LDH MDH
G6PDH6
FDH GDH
4
Rm
1GOT GOT . AC
A 2002
B 2003
101
4 mol/l)M 4 s t a c k i n g buffer(50ml) A 1M HC1 48ml
Trizmabase 36.6g TEMED 0.23ml
Trizmabase 5.98g 100ml C 29.2g
BIS 0.8g 100ml F
Trizmabase 6.0g 28.8g
1000ml pH8.3 G 0.14g 100ml G' 10 g 9ml
1000ml) reservoirbuffer pH4.3 0.2M PHospHate bufferpH 7 .5 )
0.1M Tris-HCI pH8.0 0.2M Tris-HCI pH8.0 0.1M Tris-HCI pH7.0 0.2M L-L-Asparticacid 2M KOH 4.0 L-Ieucyl-B-napHthylamide 1M DL- 2M NaOH 1.0Magnesium chloride 6
0.1BPB) 0.2M Tris-0.2M Maleate,pH3.8 NAD
NADP
5 20
1530μl
10mA 3020mA
37
2 GOT
LAP SKDH ADH LDH MDH
G6PDH6
FDH GDH
4
Rm
1GOT GOT . AC
A 2002
B 2003
101
4 mol/l)M 4 s t a c k i n g buffer(50ml) A 1M HC1 48ml
Trizmabase 36.6g TEMED 0.23ml
Trizmabase 5.98g 100ml C 29.2g
BIS 0.8g 100ml
G' 10 g 9ml
1000ml) reservoirbuffer
pH4.3 0.2M PHospHate bufferpH 7 .5 )
0.1M Tris-HCI pH8.0 0.2M Tris-HCI pH8.0 0.1M Tris-HCI pH7.0 0.2M L-L-Asparticacid 2M KOH 4.0 L-Ieucyl-B-napHthylamide 1M DL- 2M NaOH 1.0Magnesium chloride 6
0.1BPB) 0.2M Tris-0.2M Maleate,pH3.8 NAD
NADP
5 20
1530μl
7GPDHFDHLDH GPDHFDHLDH
8 NativePAGE
5
1
2MDH GDH
3
103
e100GOT GOT GOTGOT
2ADH ADH ADH
E ADH1
fastF
3MDH MDH
4SKDH SKDHF
4F
5GDH GDH 2 GDH1
6LAP
7GPDHFDHLDH GPDHFDHLDH
8 NativePAGE
5
1
2MDH GDH
3
103
2ADH ADH
ADH
E ADH1
fastF
3MDH MDH
4SKDH SKDHF
4F
5GDH GDH 2 GDH1
6LAP