AGROBIOLOGICALAGROBIOLOGICAL

RESEARCHRESEARCH

AND CONSULTINGAND CONSULTING

FUNGAL DISEASES CAN RESULT FUNGAL DISEASES CAN RESULT

IN CONSIDERABLE DAMAGE TO IN CONSIDERABLE DAMAGE TO

AGRI-CULTURAL AND AGRI-CULTURAL AND

HORTICULTURAL CULTIVARS.HORTICULTURAL CULTIVARS.

UNDER CERTAIN CIRCUMSTANCES UNDER CERTAIN CIRCUMSTANCES

AND WITH AN ACCORDING AND WITH AN ACCORDING

EPIDEMIC A LOSS IN YIELD OF 80 % EPIDEMIC A LOSS IN YIELD OF 80 %

IS POSSIBLE.IS POSSIBLE.

HOW CAN THE HARVEST BE HOW CAN THE HARVEST BE

PROTECTED ?PROTECTED ?

MECHANICAL CULTIVATIONMECHANICAL CULTIVATION

USE OF RESISTANT CULTIVARSUSE OF RESISTANT CULTIVARS

USE OF EFFECTIVE PESTICIDESUSE OF EFFECTIVE PESTICIDES

FUNGAL DISEASES ADAPT FUNGAL DISEASES ADAPT

TOWARDS PESTICIDES AND TOWARDS PESTICIDES AND

RESISTANCE GENES JUST LIKE RESISTANCE GENES JUST LIKE

HUMAN PATHOGENS CAN ADAPT HUMAN PATHOGENS CAN ADAPT

TOWARDS ANTIBIOTICS.TOWARDS ANTIBIOTICS.

THIS SUBJECT AREA IS OUR THIS SUBJECT AREA IS OUR

EXPERTISE:EXPERTISE:

DISEASE DISEASE

PATHOGENSPATHOGENS

ACTIVE ACTIVE

INGREDIENTINGREDIENT

CULTIVARCULTIVAR

HAS THE PATHOGEN

ADAPTED OR BECOME

RESISTANT TO THE

FUNGICIDE ?

IS THERE AN ADAPTION

TOWARDS THE RESISTANCE

OF A CULTIVAR ?WHAT

STRATEGIES CAN

BE APPLIED IN CASE

OF AN ADAPTION ?

ARE NEW

BREEDING LINES

AVAILABLE THAT SHOW

BETTER RESISTANCE ?

EVERY YEAR WE SAMPLE EVERY YEAR WE SAMPLE

PATHOGEN SPORES ACROSS PATHOGEN SPORES ACROSS

EUROPE TO ANSWER THESE EUROPE TO ANSWER THESE

QUESTIONS.QUESTIONS.

ST. MARY ON SEA,

ENGLAND

ALSACE, FRANCE

SCOTTISH BORDER

MARCHFELD, AUSTRIA

LOCH NESS, SCOTLAND

RHINELAND, GERMANY

CAP BLANC NEZ, FRANCE

EASTCOAST, SCOTLAND

OF COURSE NOT ONLY IN PRETTY PLACES!OF COURSE NOT ONLY IN PRETTY PLACES!

THE PATHOGEN STRAINS ARE THE PATHOGEN STRAINS ARE

THEN ISOLATED IN THE THEN ISOLATED IN THE

LABORATORY AND STORED.LABORATORY AND STORED.

WE APPLY DIFFERENT METHODS WE APPLY DIFFERENT METHODS

TO ANALYSE THE PATHOGEN TO ANALYSE THE PATHOGEN

STRAINS:STRAINS:

ON LIVINGPLANT MATERIAL

in vivo

PATHOGEN PLANT

ACTIVE COMPOUND

PATHOGEN PLANT

UNDER ARTIFICIALCONDITIONS

in vitro

PATHOGENCOMPOUND

MOLECULAR-BIOLOGICAL

METHODS(EpiGene)

undigested sensitive/ItaIresistant/ItaI

FINALLY, WE EVALUATE THE FINALLY, WE EVALUATE THE

OBTAINED DATA.OBTAINED DATA.

0

20

40

60

80

100

Contr. 0,03 0,1 0,3 1,0 3,0 10,0 30,0

CONCENTRATIONS (mg/l)

% G

RO

WT

H C

OM

PA

RE

D T

O U

NT

RE

AT

ED

CO

NT

RO

L

PROBIT-ANALYSIS

EC50

QUALITATIVE RESISTANCE

Sel

ectio

nS

election

sensitive individuals resistant individuals

1 2 3 4 5

Minutes

50% WM resistant50% WM resistant

10% WM resistant10% WM resistant

100% WM sensitive100% WM sensitive

5% WM resistant5% WM resistant

DHPLC-PCR(EpiGene)

TAQMAN PCR(EpiGene)

WE PROVIDE OUR PARTNERS WITH WE PROVIDE OUR PARTNERS WITH

OUR KNOWLEDGE AND OUR KNOWLEDGE AND

INFORMATION:INFORMATION:

PLANT BREEDINGPLANT BREEDING

PLANT PLANT

BREEDINGBREEDING

CHEMICAL CHEMICAL

INDUSTRYINDUSTRY

CHEMICALCHEMICAL

INDUSTRYINDUSTRY

PLANT PLANT

BREEDINGBREEDING

ADVISORY SERVICESADVISORY SERVICES

ININ

SCHLESWIG-HOLSTEINSCHLESWIG-HOLSTEIN

LOWER SAXONYLOWER SAXONY

MECKLENBURG-VORPOMMERNMECKLENBURG-VORPOMMERN

HESSEHESSE

THURINGIATHURINGIA

SAXONYSAXONY

RHINELAND-PALATINATERHINELAND-PALATINATE

BADEN-WÜRTTEMBERGBADEN-WÜRTTEMBERG

BAVARIABAVARIA

CHEMICALCHEMICAL

INDUSTRYINDUSTRY

PLANT PLANT

BREEDINGBREEDING

ADVISORYADVISORY

SERVICESSERVICES

ININ

SCHLESWIG-HOLSTEINSCHLESWIG-HOLSTEIN

LOWER SAXONYLOWER SAXONY

MECKLENBURG-VORPOMMERNMECKLENBURG-VORPOMMERN

HESSEHESSE

THURINGIATHURINGIA

SAXONYSAXONY

RHINELAND-PALATINATERHINELAND-PALATINATE

BADEN-WÜRTTEMBERGBADEN-WÜRTTEMBERG

BAVARIABAVARIA

SO, IF YOU EVER SEE ...SO, IF YOU EVER SEE ...

... ANYWHERE IN ... ANYWHERE IN

EUROPE ...EUROPE ...

... THIS VEHICLE:... THIS VEHICLE:

DON‘T BE SURPRISED !?!DON‘T BE SURPRISED !?!

JUST GIVE US A HIGH FIVE !JUST GIVE US A HIGH FIVE !

´CAUSE YOU‘VE JUST MET´CAUSE YOU‘VE JUST MET

THE SPOREHUNTERS !THE SPOREHUNTERS !

IN THE NAME OF A MODERN, EFFICIENTIN THE NAME OF A MODERN, EFFICIENT

AND SUSTAINABLE AGRICULTUREAND SUSTAINABLE AGRICULTURE

EpiLogicEpiLogiccreated by B. Jasercreated by B. Jaser

www.epilogic.dewww.epilogic.de