audrey le gouellec laboratoire timc/the rex - umr 5525 · audrey le gouellec laboratoire timc/the...

Audrey Le GouellecLaboratoire TIMC/The Rex - UMR 5525

Wh i b i f i h ?In vivo antigen delivery inside host cell

Why using a bacteria as vector for immunotherapy?g y

Potent immunostimulant (the « Danger theory »)

Knowledge in microbiology : bacterial physiology and engineeringKnowledge in microbiology : bacterial physiology and engineering

Forbes et al. 2010

A. Le Gouellec et al. 2012 Human vaccin and immunother.

« Killed but metabolically active »(KBMA) P. aeruginosa

Amotosalen

0µM 2µM 5µM 10µM 15µM 20µMEGTAEGTA

3h post infection LDH release

Systemic response in mouseCytokine assay xMAP Technology (Luminex)

DisseminationSPECT/CT experiment

J 14 J 7 J0

Prime Boost Challenge Tumoral

J-14 J-7 J0

Challenge Tumoral Vaccination

J0 J+1 J+24

ThanksThanksProf Benoit Polack

TheRExProf. Benoit Polack

TheRExExperimental recombinant therapy

TIMC-CNRS UMR 5525

Bertrand ToussaintXavier ChauchetCharlotte Genestet INSERM, U877

Jean-Luc LenormandCharlotte GenestetLionel BerthoinYan WangHichem Chaker

Mitra AhmadiAlexis BroisatCatherine GhezziHichem Chaker

David Laurin

ANRCEA/irTSV/BBSIJulien Verove ANR

INSERM, U823Caroline Aspord

Julien VeroveFrançois CretinIna AttreeCHU G bl

pJoel Plumas CHU Grenoble

IBP/UM BEPCandice Trocme