EXPERIMENT 4Kinetics of Enzyme Reaction

Today’s Conference

• Introduction• Objectives • Methodology• Actual Results and Interpretations• Comparison with Theoretic Concepts• Conclusion

INTRODUCTIONEXPERIMENT 4: Kinetics of Enzyme Reaction

Enzyme Kinetics• quantitative measurement of the rates of enzyme-

catalyzed reactions• systematic study of factors that affect these rates

K1

K-1

K2

E + S ES E + P

Factors Affecting Kinetics

Activators and Inhibitors

Substrate Concentration

Michaelis-Menten Kinetics

• one of the simplest and most widely-known models of enzyme kinetics

• shows the relationship between initial reaction velocity Vi and substrate concentration [S]

Leonor Michaelis Maud Menten

Lineweaver-Burke Plot

• graphical linearization of the Michaelis-Menten Kinetics to be able to determine Km and Vmax

• double reciprocal plot

Hans Lineweaver Dean Burke

Enzyme Kinetics

Michaelis-Menten Lineweaver-Burke