immunoistochimica - home - people.unica.it...(b) indirect immunc:ytochemical technique using...
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IMMUNOISTOCHIMICA
COESISTENZA
TRACCIANTI
•• Peptidi:Peptidi: sono presenti in piccole sono presenti in piccole quantitquantitàà
•• Canali ionici:Canali ionici: espressi in basse espressi in basse concentrazioni, presenza di concentrazioni, presenza di subtipisubtipi
•• Recettori:Recettori: espressi in basse espressi in basse concentrazioni, presenza di concentrazioni, presenza di subtipisubtipi
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IMMUNOISTOCHIMICA
FISSAZIONE TESSUTO
TAGLIO (CRIOSTATO, VIBRATOMO)
INCUBAZIONE CON ANTICORPO PRIMARIO
INCUBAZIONE CON ANTICORPO SECONDARIO
VISUALIZZAZIONE DELLA REAZIONE
QUANTIZZAZIONE
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Equipment required to perfuse small rodents: a) two glassflasks; two sets ofplastic tubing with a flow regulator; b) a plastic “Y” connector; a cannula and a haemostatic clamp with a drilled hole.
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F
F
Diretto
Indirettofluorescina
IndirettoPAP
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TECNICA DEL PAP
goat
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Colorazione con PAP
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Astroglia attivata
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ISTOCHIMICA
TRACCIANTI ANTEROGRADI
TRACCIANTI RETROGRADI
IMMUNOISTOCHIMICA
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ENKEFALINASI RECETTORI mi RECETTORI delta
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