iufro division 5 conference 5.07.b energy from biomass / chemicals from wood chi-lin wu and...
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IUFRO Division 5 Conference
5.07.B Energy from biomass /
Chemicals from wood
Chi-Lin Wu and Shang-Tzen Chang
National Taiwan University
Isolation and Identification of Antifungal Constituents from the Heartwood Extracts of Taiwan Hinoki (Chamaecyparis obtusa var. formosana)
C.-L. Wu & S.-T. Chang
IUFRO Division 5 Conference5.07.B Energy from biomass / Chemicals from wood
School of Forestry and Resource Conservation, National Taiwan UniversityEmail: [email protected]
National Taiwan University
Aim of work and methods Taiwan hinoki (Chamaecyparis obtusa var. formosana) is
an endemic species in Taiwan and its timbers are well
recognized for their natural decay resistance.
Isolation and identification of antifungal constituents from
the heartwood extracts of Taiwan hinoki were carried out
in this study.
Bioassay-guide fractionationAgar dilution assay
Column chromatography
HPLC
MS, 1H-NMR, 13C-NMR, and 2D-NMR analyses
(100 μg/mL) 0 20 40 60 80 100
Menthane-1,4-diol
Menthane-1,8-diol
α-Cadinol
δ-Cadinol
T-Muurolol
Hinokiol
Hinokione
Sandaracopimaric acid
β-Sitosterol
Stigmasterol
Antifungal index (%)
L. betulina
L. sulphureus
Results
Ethanolic Extract
Taiwan Hinoki Heartwood
n-Hex fr. EtOAc fr. EtOH fr.
CFH 1 CFH 6 CFH 12
Extracted by EtOH
Liquid-liquid partition
Column chromatography : Silica gel, n-Hex / EtOAc / MeOH
HPLC separation : Si-60 column, n-Hex/EtOAc
～ ～
H
H
OH
OH
H
H
OHH
H
OH
COOH COOH
O
OHOH
OH
OH
OH
OH
OH
0
25
50
75
100
Crude Hex EA EtOH
An
tifu
nga
l in
dex
(%
)
L. betulina
L. sulphureus
(500 μg/mL)
T-Muurolol α-Cadinol
H
H
OH
OH
H
H
OHH
H
OH
Control α-Cadinol
Heartwood extract exhibited an excellent antifungal activity against brown-rot fungus L. sulphureus and white-rot fungus L. betulina.
According to MS, 1H-NMR and 13C-NMR analyses, 16 compounds were identified, namely α-cadinol, -cadinol, T-muurolol, -costol, hinokiol, hinokione, sandaracopimaric acid… etc.
α-Cadinol and T-muurolol efficiently inhibited the growth of wood-rot fungi.
Conclusions
National Taiwan University