Upload File

p53- and drug-induced apoptotic responses mediated by bh3-only proteins puma and noxa p53- and...

  • Home
  • Documents
  • P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins
13
P53- and Drug-Induced P53- and Drug-Induced Apoptotic Responses Apoptotic Responses Mediated by BH3-Only Mediated by BH3-Only Proteins Puma and Noxa Proteins Puma and Noxa Villunger, A. et al. Science, 2003 Flávia Gama Gomes Leite

Upload: internet

Post on 17-Apr-2015

106 views

Category:

Documents


0 download

Report

Tags:

TRANSCRIPT

Page 1: P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins

P53- and Drug-Induced P53- and Drug-Induced Apoptotic Responses Mediated Apoptotic Responses Mediated by BH3-Only Proteins Puma by BH3-Only Proteins Puma

and Noxaand Noxa

Villunger, A. et al. Science, 2003

Flávia Gama Gomes Leite

Page 2: P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins

p53 – ciclo celular e p53 – ciclo celular e apoptoseapoptose

p53

p21

Parada do ciclo celular

Mais de 16 genes alvos

Apoptose ?

Page 3: P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins

Possíveis Genes AlvosPossíveis Genes Alvos

Puma (p53-upregulated modulator of apoptosis)

Noxa (Noxius stress)

Domínio BH3

Page 4: P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins

Construção de NoxaConstrução de Noxa-/--/-

Page 5: P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins

NoxaNoxa-/--/-

Northern blot

Agarose - controle

Southern blot

PCR

Southern blot

Page 6: P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins

NoxaNoxa-/--/-

Estímulo dependente de p53

Page 7: P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins

NoxaNoxa-/--/-

Estímulos independentes de p53

Estímulos dependentes de p53

Page 8: P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins

Construção de PumaConstrução de Puma-/--/-

Page 9: P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins

PumaPuma-/--/-Southern blot

PCR

Southern blot - sonda exon 2

RT- PCR

Northen blot

Page 10: P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins

PumaPuma-/--/-

genotóxico

Page 11: P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins

Independente de p53

Page 12: P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins

PumaPuma-/--/-

Page 13: P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins Puma and Noxa P53- and Drug-Induced Apoptotic Responses Mediated by BH3-Only Proteins

Puma-/-


Novocastra Lyophilized Rabbit Polyclonal Antibody p53 ... · NCL-p53-CM1 Page 2 Novocastra TM Lyophilized Rabbit Polyclonal Antibody p53 Protein (CM1) Product Code: NCL-p53-CM1 Intended

BH3-only protein genes in mice and rats mediated ... · the expression of the BH3-only protein and other apoptotic/cell death–related genes in all of the 5 types of cells/cell lines

CDK Inhibitors Upregulate BH3-Only Proteins to Sensitize ... fileTherapeutics, Targets, and Chemical Biology CDK Inhibitors Upregulate BH3-Only Proteins to Sensitize Human Myeloma

Dissecting the apoptotic pathway of p53 - ddd.uab.cat · PDF fileDissecting the apoptotic pathway of p53 Gasol Garcia, Ana Universitat Autònoma de Barcelona, Bellaterra Abstract P53

Orphan Nuclear Receptor NR4A1 binds a novel protein ...and the suppression of anti -apoptotic members of the family. For example, the BH3 domains of B im , B id and P uma trigger conformational

Deregulation of p53/survivin apoptotic markers correlated ... · PTEN expression in pterygium neoplastic cells Kallirroi Konstantopoulou1*, Evangelos Tsiambas2,3*, Evangelia Baliou3,

Involvement of Apoptotic Protease Cascade for Tissue ... · Involvement of Apoptotic Protease Cascade for Tissue Destruction in Sjögren’s Syndrome Y. Hayashi et al.: Apoptotic

TCTP contains a BH3-like domain, which instead of

BALDER BH3BH3 HELMET AND HARD HAT ASSEMBLY / BH3 HH - version of BH3 helmet with Safety cap concept / If you have purchased the BH3 welding helmet in combination with hard hat, the

Prosurvival AMBRA1 turns into a proapoptotic BH3-like

Prohibitin attenuates colitis-associated tumorigenesis in ... · Prohibitin attenuates colitis-associated tumorigenesis in mice by modulating p53 and STAT3 apoptotic responses Arwa

BIRO1, a Cell-Permeable BH3 Peptide, Promotes ... · BH3 peptide–based approach uses the prodeath BH3 min-imal death domains to re-establish mitochondrial sensitivity in tumoral

Installation Manual for 3M ScaleGardmultimedia.3m.com/mws/media/1215164O/3m-scalegard... · to pair with the BH3-NB, BH3-NB-NPT or the BH3-NB-BSP heads. Cartridge must be placed on

DNA-PK suppresses a p53 independent apoptotic response to ...authors.fhcrc.org/90/1/GurleyKempManuscript_102908.pdf · DNA-PK suppresses a p53 independent apoptotic response to DNA

Rab35FunctionsinAxonElongationAreRegulatedby P53

C-Terminal p53 Palindromic Tetrapeptide Restores Full ...ym190/4R paper.pdfInduction of Apoptosis by a p53 Peptide 1 C-Terminal p53 Palindromic Tetrapeptide Restores Full Apoptotic

Parts Breakdown Switch cover *Receiver Antenna pipe …...BS3 BD7 BC4 BM4 BH6 BH3 D1 BC4 BD7 BD6 B5 BC5 BD5 BH2 C6 BP1 F8 BA7 BB1 BC1 C10 C1 BM3 BA3 BB1 BC3 BM2 BA2 BH3 BB4 BD7 BH3

Chrysin abrogates cisplatin-induced oxidative …...Chrysin abrogates cisplatin-induced oxidative stress, p53 expression, goblet cell disintegration and apoptotic responses in the

BH3 Helix-derived Biophotonic Nanoswitches Regulate ...4.2 Retention of molecular components of the apoptotic pathway in a permeabilised cell system 7 4.3 Imaging overall mitochondrial

Structure of the BH3 Domains from the p53-Inducible … · Structure of the BH3 Domains from the p53-Inducible BH3-Only Proteins Noxa and Puma in Complex ... Multiple BH domain-bearing

Service Division - Neuron Bio · propidium iodide staining and flow cytometry . Apoptotic DNA fragmentation is measured in an apoptotic cell model. • Analysis of anti-apoptotic

HOXC6IsDeregulatedinHumanHeadandNeckSquamous ... · 35678 journalofbiologicalchemistry volume287•number42•october12,2012 from the BH3 domains of pro-apoptotic proteins promotes

APOPTOSIS SIGNALING PATHWAY - Bio Cusa · Apoptosis Pro-apoptotic genes DNA Damge Pro-apoptotic genes c-jun AP1 p53 Fas Fas-L Bim HRK p53 ATM Bak Bax PIDD Bid Apaf-1 p53AIPI Fas TRAILR2

Identification of Apoptotic Proteins

Resistance to Chemotherapy in Breast Cancer · 2017-10-10 · Resistance to Chemotherapy in Breast Cancer: Potential role of p21B, p27 and the p53 apoptotic pathway Ranjan Chrisanthar

CDK Inhibitors Upregulate BH3-Only Proteins to Sensitize Human ... - Cancer … · Therapeutics, Targets, and Chemical Biology CDK Inhibitors Upregulate BH3-Only Proteins to Sensitize

The p53 target Wig-1 regulates p53 mRNA stability through ... › content › pnas › 106 › 37 › 15756.full.pdf · The p53 target Wig-1 regulates p53 mRNA stability through an

The BCL2 Family: Key Mediators of the Apoptotic Response to … · 2015. 4. 19. · drial or intrinsic apoptotic response. Recent advances in our understanding of apoptotic signaling

Apoptotic mechanisms in the neonatal brain following ... · Apoptotic mechanisms in the neonatal ... epilepsi och utvecklingsstörning ... Apoptotic mechanisms in the neonatal brain

Aberrant Death Pathways in Melanoma · scription factor for several pro-apoptotic proteins. p53 induced apoptosis can be activated via intrinsic and the extrinsic cellular pathways

Mutual Regulation of Bcl-2 Proteins Independent of the BH3 ... · Mutual Regulation of Bcl-2 Proteins Independent of the BH3 Domain as Shown by the BH3-Lacking Protein Bcl-x AK Michael

Ankaferd hemostat: from molecules to medicine · activates the p53 apoptotic pathway and causes apoptosis [11]. Protein–protein interaction networks are important interactions in

Open Access The p53-dependent apoptotic pathway of breast … · 2017-04-11 · [16,17]. In the case of di-aziridinyl-substituted quinone, this highly cytotoxic bifunctional alkylating

P53 Tumor Supressor Protein Lab p53 - PowerPoint on p53...Click on The p53 Gene and Cancer and then play. Eight slides explain the structure and function of p53. Click on Using p53

A Novel Bioavailable BH3 Mimetic Efficiently Inhibits Colon … · the BH3 mimetic mediates its antitumor effects and facilitates its clinical development. Materials and Methods