Pasteurella

• Disease

• Treatment

• Prevention

Disease

• Bacterial--gram negative

• Pasteurella, (Mannheimmia) haemolytica

– 75% of isolations

• Pasteurella Multocida

– 25% of isolations

Disease--Virulence Factors

• Leukotoxin

• Capsular Polysaccharide--CPS

• Fimbriae

• Iron Regulated Outer Membrane Protein--IROMP

• Surface Antigen

Diagnostics

• Serum Neutralization

• Agglutination

• ELISA

Challenge Models

• Transthoracic

• Endobronchial

• Intratracheal

References

• Data--P-Values

• Data--General

• Personal Communications

Reliant HM

• Efficacy

• Safety

Efficacy--Background

• Dairy Bull Calves• Ontario, CANADA• 150-350#• 10 weeks upon arrival• Acclimated 20-43 days• Conditioned: Preship Micotil (Pm) or

NuFlor (Ph2). PreVac: NuFlor and Chlortetracycline

Study

• 2 ml dose SC on day 0

• Challenged 21 days post vaccination

Challenge

• P. haemolytica

• 25ml of 1.76 X 10-10 CFU/ml, 4.4 X 10-11 CFU/dose, intratracheal

Challenge

• P. multocida

• 120 ml of 5.2 X 10-9 CFU/ml, 6.2 X 10-11 CFU/dose, intratracheal

Result--% pnemonic tissue (P. haemolytica)

• Test vx. 15.74% (+/-2.83)

• Placebos: 32.85% (+/-3.81)

• P < 0.0001

Clinical signs

• Febrile controls vs. vx. P <0.0001• Resp. rate inc. controls vs. vx. P <0.0001• Injection site reactions vx. Vs. controls P<

0.0001– D 7 nodules in 31.8%– D 14 nodules in 13.5%– D 21 palpation nodules in 4.6%– D 60 palpation nodules in 0%

Leukotoxin neutralizing antibody titers--P.heamolytica• Vaccinates

– D 0 : 2.76 vx. Day

– D21 : 5.35 @ challenge

• Controls

– D 0 : 3.22

– D 21 : 4.61

Conclusion

• Significant correlation between Abs. Vs. pneumonia P< 0.0220

Agglutinating antibody titers--P. haemolytica

• Vaccinates

– D 0: 5.28

– D 21: 6.52

• Controls

– D 0: 5.04

– D 21: 5.50

Conclusion

• Significant difference between groups p<0.0012

• Significant correlation between Abs vs. pneumonia P< 0.0004

Results--P. multocida % pneumonic tissue

• Vacc.: 30.52 % (+/- 1.96)

• Placebos: 43.70% (+/-2.10)

• Statistically significant P<0.0001

Clinical signs

• Febrile

– Controls vs. vx. P< 0.0315

• Resp., rate

– Controls vs. vx. P<0.0021

• Injection site

– Vx. Vs. controls P<0.0001