Research in a nutshell

Metalloporphyrins and other complexes as ionophores for

hydrophilic anions

• Synthesis of novel compounds (mainly Zr- and Al-derivatives)

• Studies on the selectivity and other paramaters of developed ISEs

F-SCN-

I-

Cl-

Ace-

NO2-BF4

-

I3-

CN-

Sal-

Cu2+ Zn2+

Mn3+ Fe3+ Rh3+

Mn3+ In3+ Tl3+

Mn3+

Mn3+

Mn3+MoO3+Cr3+ Lu3+ Sn4+

Rh3+Co3+

Ga3+ Zr4+ Al3+ Sc3+

Zr4+

Metals Appliedour group

Metalloporphyrins as ionophores

N

N

N

N

R1R1

R2

R1

R1

R2

R1 R1

R2

R1

R1

R2

Mn+

R1 - R2 - H

R1 - H R2 -

R1 - H R2 -

R1 - H R2 -

Cl

Cl

R1 - H R2 - NH

O

Application of developed ISEs in FIA systems

• Determination of acetylcholine

• Determination of voltaile fatty acids

• Determination of pesticides

Sample (Enzyme substrate, inhbitor, activator)

Enzymatic flow-throughreactor

Detector (acetateselective electrode)

Gly buffer(pH 3,0)

Phosphate buffer (pH 7,4)

Sample (Enzyme substrate, inhbitor, activator)

Enzymatic flow-throughreactor

Detector (acetateselective electrode)

Gly buffer(pH 3,0)

Phosphate buffer (pH 7,4)

Silicon-based transducers

Working electrode3-electrode transducer

•vacuum deposited gold electrodes

•smooth electrode surface facilitate DNA monolayer stability

I

II

IIII – bare gold electrode

II – ssDNA

III - dsDNA

DNA receptor layers

• New redox markers for DNA sensors

• Silicon-based transducers for DNA sensors

• DNA receptor layers for determination of heavy metal cations

• Aptasensors

s s s s

Metalacarboranes as DNA redox labels

M = Fe3+, Cr3+, Co3+

M

DNA for heavy metals determination

eTeT

methylene blue – redox marker

UO22+ - LDL 30 nmol/L

Hg2+ - LDL 5 nmol/L

Pb2+ - under investigation

Metalloporphyrins for labeling of proteins

• Various transduction techniques

-voltammetry

-spectrophotometry

-spectrofluorimetry

Properties of metalloporphyrins

Influence of various proteins (antibodies) on the obtained signals

Quantum Dots as labels • Modification - hydrophilization and

functionalization of surface• Stability • Determination (influence of chosen species

(proteins) on obtained optical and electrochemical signals, elimination od undesired effects)

Other interests…

Interactions between DNA, aptamers, peptides, proteins and...

• SPR

• QM

Modification of gold surface (chemisorption, covalent bonding, adsorption)