strawberry dna extraction. dna: basics deoxyribonucleic acid genetic material ◦ chain of molecules...
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Strawberry DNA Strawberry DNA ExtractionExtraction
DNA: BasicsDNA: BasicsDeoxyribonucleic acid Genetic material
◦ Chain of molecules linked together◦ DNA contains instructions for traits
Wrapped tightly around “histones”
Forms chromosomesHoused in nucleus
DNA: BasicsDNA: Basics
DNA: StructureDNA: StructureDouble-helix (twisted ladder)Sugar and phosphate backboneNitrogen basesSubunit: “nucleotide”
DNA: ReplicationDNA: ReplicationSpecial pairing between nitrogen
basesNew strand is copy of old strand
DNA Extraction: Step 1DNA Extraction: Step 1Break the cells open (using force)
DNA Extraction: Step 2DNA Extraction: Step 2Dissolve the cell membrane and
nuclear envelope (using detergent/soap)
Current Protocols in Protein SciencePublished by John Wiley & Sons, Inc.
DNA Extraction: Step 3DNA Extraction: Step 3Remove proteins that are bound
to DNA (using salt to dissolve them)
DNA Extraction: Step 4DNA Extraction: Step 4Precipitate DNA (with ethanol)
DNA Extraction: Why?DNA Extraction: Why?Species identification
VS.
DNA Extraction: Flow DNA Extraction: Flow chartchart
Bag already has extraction solution.
Secure cheesecloth to beaker.
Do NOT disturb tube!
Collect DNA.
Gently pour 20 mL ethanol into tube.
Transfer liquid to test tube.
Filter liquid through cheesecloth into beaker.
Mix by rocking bag slowly.
Place bag on ice for 1 minute.
Crush fruit for 5 minutes.
Squeeze out air and close bag.
Add fruit to zip-lock bag.
DNA Extraction: Flow DNA Extraction: Flow chartchart
Bag already has _______________ solution.
Secure ________________ to beaker.
Do NOT disturb tube!
Collect _______!
Gently pour ____ mL ethanol into tube.
Transfer liquid to ______ ______.
Filter liquid through cheesecloth into beaker.
Mix by rocking bag slowly.
Place bag on _______ for 1 minute.
Crush fruit for ____ minutes.
Squeeze out air and close bag.
Add fruit to zip-lock bag.