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Dr Estrella Luna-Diez @ELunaDiez lunas-research.com Priming Trees For Self-Defence BIFoR Annual Conference 30 th January, 2019 Food and Environment Research Agency, via Wikimedia Commons

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Page 1: via Wikimedia Commons Priming Trees For Self …...attack ty MAINTENANCE Next generation Pseudomonas syringae Epigenetic mechanisms Luna et al & Slaughter et al (2012), Plant Physiology,

Dr Estrella Luna-Diez@ELunaDiez

lunas-research.com

Priming Trees For Self-DefenceBIFoR Annual Conference

30th January, 2019

Food and Environment Research Agency, via Wikimedia Commons

Page 2: via Wikimedia Commons Priming Trees For Self …...attack ty MAINTENANCE Next generation Pseudomonas syringae Epigenetic mechanisms Luna et al & Slaughter et al (2012), Plant Physiology,

Career in Plant Pathology

Plant Pathology

Timeline

2006 2008

BEng and MEng in Agronomy

2012

PhD in Biological Sciences

2015

PDRA

2016

Research Fellow

2017

Future Leader Fellow

Lectureship

Phytophthorainfestans

Botrytis cinerea

Panama disease

Fusarium oxysporum

Filamentous pathogens

Late blight

Grey mould

Hymenoscyphus fraxineus

Ash Dieback

Phytophthora ramorum

Sudden Oak Death

Between 10-23% of global agricultural loss

can be attributed to fungi and oomycetes Powdery mildew

Erysiphe alphitoides

Food and Environment Research Agency, via Wikimedia Commons

Joseph O'Brien, USDA Forest Service via Wikimedia Commons

Liberato et al 2006, via Wikimedia Commons

Page 3: via Wikimedia Commons Priming Trees For Self …...attack ty MAINTENANCE Next generation Pseudomonas syringae Epigenetic mechanisms Luna et al & Slaughter et al (2012), Plant Physiology,

The Plant Immune System: A Powerful Control Strategy

Plants defend themselves extraordinarily

Constitutive barriers

Antimicrobial compounds

Cell wall defence

Hormones

ROS

SA

JA

Innate immunityPriming of defence

Page 4: via Wikimedia Commons Priming Trees For Self …...attack ty MAINTENANCE Next generation Pseudomonas syringae Epigenetic mechanisms Luna et al & Slaughter et al (2012), Plant Physiology,

Priming of defence

Plants defend themselves extraordinarily

de

fen

ce

in

ten

sit

y

time

priming

primed

un-primed

attack

PRIMING: “An increased sensitivity to certain stimuli due to prior experience”

Page 5: via Wikimedia Commons Priming Trees For Self …...attack ty MAINTENANCE Next generation Pseudomonas syringae Epigenetic mechanisms Luna et al & Slaughter et al (2012), Plant Physiology,

Stimuli of priming

Plants defend themselves extraordinarily

de

fen

ce

in

ten

sit

y

time

priming

primed

un-primed

attack

PRIMING: “An increased sensitivity to certain stimuli due to prior experience”

Chemicals

Root microbes

Pathogens

volatiles

Page 6: via Wikimedia Commons Priming Trees For Self …...attack ty MAINTENANCE Next generation Pseudomonas syringae Epigenetic mechanisms Luna et al & Slaughter et al (2012), Plant Physiology,

Botrytis cinerea

How durable is defence priming?

time

Primed

priming

un-primed

attackd

efe

nc

e i

nte

ns

ity

MAINTENANCE

Weeks after treatment

5 weeks 6 weeks

Luna et al (2014) Frontiers in Plant Science

Bruce et al (2017) Crop Protection

Hyaloperonosporaarabidopsidis

Luna et al (2016) Plant Disease

Botrytis cinerea

12 weeks

Wilkinson et al (2018) Plant Pathology

Luna et al (2014), Nature Chemical Biology

Page 7: via Wikimedia Commons Priming Trees For Self …...attack ty MAINTENANCE Next generation Pseudomonas syringae Epigenetic mechanisms Luna et al & Slaughter et al (2012), Plant Physiology,

T0

How durable is defence priming?

time

Primed

priming

un-primed

attackd

efe

nc

e i

nte

ns

ity

MAINTENANCE

Next generation

Pseudomonassyringae

Epigenetic mechanisms

Luna et al & Slaughter et al (2012), Plant Physiology, Luna and Ton (2012) Plant Signalling and Behaviour, Stassen et al (2018) Scientific Reports

Page 8: via Wikimedia Commons Priming Trees For Self …...attack ty MAINTENANCE Next generation Pseudomonas syringae Epigenetic mechanisms Luna et al & Slaughter et al (2012), Plant Physiology,

Priming in forest trees

Can trees be primed?

What are the mechanisms?

Is the resistance maintained for long periods of time?

Epigenetics?

Transgenerational resistance?

Is there genetic variability?

Neighbouring effects?

How does CO2 impact priming?

Hormones,cell-wall defence?

New ones?

Tolerance?

Page 9: via Wikimedia Commons Priming Trees For Self …...attack ty MAINTENANCE Next generation Pseudomonas syringae Epigenetic mechanisms Luna et al & Slaughter et al (2012), Plant Physiology,

“Thinking Higher”

International symposium in Forest Pathology:

Towards biosecurity of forest trees

Confirmedspeakers:

- Prof Dawn Arnold- Prof Sarah Gurr

- Prof Sandra Denman- Prof Luis Sampedro - Dr Alejandro Solla- Dr Victoria Pastor

11th July, 2019Edgbaston Campus

University of Birmingham

Page 10: via Wikimedia Commons Priming Trees For Self …...attack ty MAINTENANCE Next generation Pseudomonas syringae Epigenetic mechanisms Luna et al & Slaughter et al (2012), Plant Physiology,

Dr Estrella Luna-Diez@ELunaDiez

lunas-research.com

Priming Trees For Self-DefenceBIFoR Annual Conference

30th January, 2019

Food and Environment Research Agency, via Wikimedia Commons


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