Quickstart: Biplanar fluoroscopy/videoradiography equipment 3/3/161. Turn on breakers 1 &2 (up is on) and power strips 1 & 2 (Figure 1).2. Switch on timing box (Figure 1, up is on, green light illuminates).3. Turn on power to both panels in control booth (Figure 2).4. Turn filter F1 on for both panels (Figure 2, green light illuminates).5. Open ProCapture software; use “configure” button (top right) to set number of cameras & fps.6. Check that beam type is in Radiographic mode and reduce technique to 40kV and 10mA.7. Turn knobs on the collimation panel (Figure 3, bottom center) to desired magnification.8. Push & hold the “close iris” button on both A & B collimator panels for 20 seconds (Figure 3).9. Press and hold the foot pedal to produce x-rays; images will show up on computer monitor.10. While pressing pedal, open both iris collimators until only the iris leaf edges can be seen.11. Set beam mode to “warm up” (Figure 2) on both panels.12. Hold the “prep” button (“manual engage”, Figure 2) until it blinks.13. While still holding prep, press the x-ray button (manual engage, figure 2) for 3 seconds.14. Repeat prep/xray warm up until Heat Units reach 5 or more.15. Set beam mode (Figure 2) to strobe for 1-100fps, or continuous for 125+ fps.16. Switch beam type (Figure 2) to “radiographic” and set kV and mA as desired for data collection.17. Place perforated metal grids onto both Image Intensifiers. 18. In ProCapture software, under “configure” and “event save”, set save destination.19. In ProCapture click “Idle”, then “Live”, then press foot pedal to view x-ray images of grids. 20. If image is too dark or bright, increase or decrease shutter speed, NOT x-ray energy.21. While pressing foot pedal, click “Save single frame”, and then release foot pedal.22. Remove grids and place cube in field of view.23. While pressing foot pedal, click “Save single frame”, and then release foot pedal.24. Move cube into a different orientation in the field of view.25. While pressing foot pedal, click “Save single frame”, and then release foot pedal.26. Move cube to yet another orientation in the field of view.27. While pressing foot pedal, click “Save single frame”, and then release foot pedal.28. Move cube to a fourth orientation in field of view – trust me, your precision will thank me later.29. While pressing foot pedal, click “Save single frame”, and then release foot pedal.30. Position subject into field of view WITHOUT TOUCHING the emitters or intensifiers.31. If the emitters or intensifiers get touched, re-do grid images.32. If re-doing grids, also take cube images. Yes, all of them; there are no shortcuts in good science. 33. Once experimental set up is ready and subject is in view, click “record” in ProCapture.34. Gather your data. Red text denotes the buffer duration used/remaining; watch it carefully. 35. Between trials, “event count” should be even. If it is odd, hit space bar for manual trigger.36. Hit “stop/idle” to end recording, and then “save all events” to save between sequential triggers.37. Once all events are saved, take additional cube images followed by a grid image.38. Post-data cube images will be useful when you unwittingly bumped the machines. Klutz!39. Turn off panels first (Figure 2), then shut off timing box, power strips, and breakers (Figure 1).40. Copy data from the ProCapture computer to midway or an external hard drive.41. Delete data from PorCapture data within 24 hours, or it will be deleted for you. 42. Seriously, we will delete data from the ProCapture drive often, and we will not ask you first.

Figure 1

Figure 2

Figure 3